sciencegoodding, cooperating with the oregon experiment station, corvallis, a mummiedcone of the...

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VOL. 79, No. 2060 days was 29 grams for each rat. The growth curve for those on the non-sterile dry plants was closely parallel. The rats fed on the fresh Lemna put on weight faster and gained an average of 37 grams, suggesting that part of the vitamin was destroyed in the drying process. The equal rate of growth made by the two groups receiving sterile and non-sterile plants not only shows that vitamin A can be formed in plants which have been free from all influence of microorganisms for hundreds of generations, but also is an indication that there is little or no difference in quantity pro- duced. Further, while the non-sterile Lemna were grown by Mr. E. E. Frahm for the most part in sun- shine, the sterile plants were produced altogether under artificial light-electric light bulbs in this case furnishing the illumination.8 From these results it appears that if conditions are favorable to the growth of green plants, the absence of microorganisms does not affect the forma- tion of vitamin A and also that variation in light within limits has little effect. Virtanen and v. Hausen believe that the form or availability of nitro- gen may be one of the controlling factors. NORMAN ASHWELL CLARK B. H. THOMAS THE DEPARTMENT OF CHEMISTRY IOWA STATE COLLEGE FUSARIUM BACTRIDIOIDES SP. NOV., ASSO- CIATED WITH CRONARTIUM IN March, 1932, Arthur Hinckley at the Desert Botanical Laboratory, Tucson, Arizona, sent to L. N. Goodding, cooperating with the Oregon Experiment Station, Corvallis, a mummied cone of the Chihuahua pine (Pinus leiophylla Sehlechtendahl and Chamisso) from the Chiricahua Mountains in Arizona. The cone had been attacked by the cone blister rust (Cronartium conigenum Hedgeock and Hunt). Mr. Goodding found this rust to be thoroughly parasitized by a species of Fusarium. The spores of this Fu- sarium were viable, and cultures were easily obtained from spore dilutions on potato agar. Through the courtesy of S. M. Zeller, Oregon Experiment Station, cultures of Goodding's Fusarium were transmitted to the undersigned, who has found the organism to be a new species in the Section Discolor. The diagnostic description is presented as follows: Fusarium bactridioides Wollenweber, nova species, (Sectio Discolor, subs. Trichotheeioides): Conidia minora aerio mycelio ex albo incarnato instrata, numerosa, ovoidea v. ellipsoidea, continua 7.3x3.7 plerumque 5.9-9 x 3.4-3.9 (5-11 x 3-5), rarius 1-( (2-) ) sept. 13 x 4.7 pler. 11-14x 4.2-5.1 (7-21 x 3-6.5), majora 8 Clark, Iowa State Jour. Sci., 7: 13-16, 1932. in sporodochiis v. pionnote roseo-aurantiaca nec non in- strata, pulveracea, inearnata, stromate plus minusve atro-violacea saepe discolorata, alia cylindrica v. fusifor- mia utrinque obtusa, recta v. curvula, alia fusoideo- subfalcata utrinque ellipsoideo-conica, apice interdum leniter constricta, basi apiculata, raro subpedicellata, 3-5, rarius 6-, rarissime 7-11 septata; 3-sept. 32 x 5.6, pl. 24-26 x 5.2-6 (17-47 x 4.5-7); 5-sept. 41 x 6 pl. 31-50 x 5.5-6.7 (25-60 x 4.8-8); 7-sept. 46 x 6.2 pl. 40-50 x 5.5- 6 .7 (30-70 x 5-8). Chlamydosporae interealares sparsae. Hab. ad conum Pini leiophyllae Schleehtendahl & Chamisso, Arizona, socio Cronartio conigeno et ad cor- tices Pini monticolae socio Cronartio ribicola, et Pini contortae socio Cronartio harknessii et Cronartio filamen- toso, Oregon. Obs. Fungus a Fusario trichothecioide recedit macro- conidiis crassioribus, interdum numero majori septorum idoneis, colore atro-violaceo stromatis, etc. Fusarium bactridioides occurs naturally on diseased cone tissues produced by Cronartium conigenum. L. N. Goodding, however, has demonstrated its abiliity to attack other pine rusts by successful inoculations on Cronartium ribicola, C. harknessii and C. filamen- tosum. The following note by Mr. Goodding is of interest in connection with the behavior of this Fusarium: Inoculations with F. bactridioides were made on blister rust cankers on western white pine (Pinus monticola) in the region of Rhododendron, Clackamas County, Oregon, in July, 1932, and in four localities in Idaho and two in Oregon in July, 1933. Several reports have been re- ceived from Crystal Creek, Idaho, of numerous actively sporulating Fusarium infections on blister rust cankers which undoubtedly resulted from the inoculations made there. At Eagle Creek, Oregon, where blister rust attack is very abundant, fully 80 per cent. of the trees sprayed with Fusarium spores in July, 1933, showed all the blis- ter rust cankers parasitized by the Fusarium by late October. In the case of July, 1933, inoculations on trees of Pinus contorta infected with Cronartium harknessii at Summit Meadows, Clackamas County, and with C. fila- mentosum near Tilly Jane Creek, Hood River County, Oregon, the Fusarium was found actively developing on both rusts by the latter part of October. All blister rust cankers inoculated in 1932 were dead by July, 1933. There is no doubt about the effectiveness of F. bac- tridioides in destroying Cronartium ribicola once it at- tacks the cankers. H. W. WOLLENWEBER BERLIN-DAHLEM BOOKS RECEIVED COKER, WILLIAm C. and HENRY R. ToTrN. Trees of the Southeastern States. Pp. 399. Illustrated. Univer- sity of North Carolina Press. $2.00. MACBRIDE, THOMAS H. and G. W. MARTiN. The Myxo- mnycetes. Pp. 338. 21 plates. Macmillan. $6.00. NOWLAN, FaRIalCK S. Analytic Geonsetry. Second edi- tion. Pp. xii + 352. Illustrated. McGraw-Hill. $2.25. 572 SCIENCE

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Page 1: SCIENCEGoodding, cooperating with the Oregon Experiment Station, Corvallis, a mummiedcone of the Chihuahua pine (Pinus leiophylla Sehlechtendahl and Chamisso) from the Chiricahua Mountains

VOL. 79, No. 2060

days was 29 grams for each rat. The growth curvefor those on the non-sterile dry plants was closelyparallel. The rats fed on the fresh Lemna put onweight faster and gained an average of 37 grams,suggesting that part of the vitamin was destroyed inthe drying process.The equal rate of growth made by the two groups

receiving sterile and non-sterile plants not only showsthat vitamin A can be formed in plants which havebeen free from all influence of microorganisms forhundreds of generations, but also is an indicationthat there is little or no difference in quantity pro-duced. Further, while the non-sterile Lemna weregrown by Mr. E. E. Frahm for the most part in sun-shine, the sterile plants were produced altogetherunder artificial light-electric light bulbs in this casefurnishing the illumination.8From these results it appears that if conditions

are favorable to the growth of green plants, theabsence of microorganisms does not affect the forma-tion of vitamin A and also that variation in lightwithin limits has little effect. Virtanen and v.Hausen believe that the form or availability of nitro-gen may be one of the controlling factors.

NORMAN ASHWELL CLARKB. H. THOMAS

THE DEPARTMENT OF CHEMISTRYIOWA STATE COLLEGE

FUSARIUM BACTRIDIOIDES SP. NOV., ASSO-CIATED WITH CRONARTIUM

IN March, 1932, Arthur Hinckley at the DesertBotanical Laboratory, Tucson, Arizona, sent to L. N.Goodding, cooperating with the Oregon ExperimentStation, Corvallis, a mummied cone of the Chihuahuapine (Pinus leiophylla Sehlechtendahl and Chamisso)from the Chiricahua Mountains in Arizona. Thecone had been attacked by the cone blister rust(Cronartium conigenum Hedgeock and Hunt). Mr.Goodding found this rust to be thoroughly parasitizedby a species of Fusarium. The spores of this Fu-sarium were viable, and cultures were easily obtainedfrom spore dilutions on potato agar. Through thecourtesy of S. M. Zeller, Oregon Experiment Station,cultures of Goodding's Fusarium were transmitted tothe undersigned, who has found the organism to be anew species in the Section Discolor. The diagnosticdescription is presented as follows:

Fusarium bactridioides Wollenweber, nova species,(Sectio Discolor, subs. Trichotheeioides): Conidiaminora aerio mycelio ex albo incarnato instrata,numerosa, ovoidea v. ellipsoidea, continua 7.3x3.7plerumque 5.9-9 x 3.4-3.9 (5-11 x 3-5), rarius 1-( (2-) )sept. 13 x 4.7 pler. 11-14x 4.2-5.1 (7-21 x 3-6.5), majora

8 Clark, Iowa State Jour. Sci., 7: 13-16, 1932.

in sporodochiis v. pionnote roseo-aurantiaca nec non in-strata, pulveracea, inearnata, stromate plus minusveatro-violacea saepe discolorata, alia cylindrica v. fusifor-mia utrinque obtusa, recta v. curvula, alia fusoideo-subfalcata utrinque ellipsoideo-conica, apice interdumleniter constricta, basi apiculata, raro subpedicellata,3-5, rarius 6-, rarissime 7-11 septata; 3-sept. 32 x 5.6, pl.24-26 x 5.2-6 (17-47 x 4.5-7); 5-sept. 41 x 6 pl. 31-50x 5.5-6.7 (25-60 x 4.8-8); 7-sept. 46 x 6.2 pl. 40-50 x 5.5-6.7 (30-70 x 5-8). Chlamydosporae interealares sparsae.Hab. ad conum Pini leiophyllae Schleehtendahl &

Chamisso, Arizona, socio Cronartio conigeno et ad cor-tices Pini monticolae socio Cronartio ribicola, et Pinicontortae socio Cronartio harknessii et Cronartio filamen-toso, Oregon.

Obs. Fungus a Fusario trichothecioide recedit macro-conidiis crassioribus, interdum numero majori septorumidoneis, colore atro-violaceo stromatis, etc.

Fusarium bactridioides occurs naturally on diseasedcone tissues produced by Cronartium conigenum. L.N. Goodding, however, has demonstrated its abiliity toattack other pine rusts by successful inoculations onCronartium ribicola, C. harknessii and C. filamen-tosum.The following note by Mr. Goodding is of interest

in connection with the behavior of this Fusarium:

Inoculations with F. bactridioides were made on blisterrust cankers on western white pine (Pinus monticola) inthe region of Rhododendron, Clackamas County, Oregon,in July, 1932, and in four localities in Idaho and two inOregon in July, 1933. Several reports have been re-ceived from Crystal Creek, Idaho, of numerous activelysporulating Fusarium infections on blister rust cankerswhich undoubtedly resulted from the inoculations madethere. At Eagle Creek, Oregon, where blister rust attackis very abundant, fully 80 per cent. of the trees sprayedwith Fusarium spores in July, 1933, showed all the blis-ter rust cankers parasitized by the Fusarium by lateOctober. In the case of July, 1933, inoculations on treesof Pinus contorta infected with Cronartium harknessii atSummit Meadows, Clackamas County, and with C. fila-mentosum near Tilly Jane Creek, Hood River County,Oregon, the Fusarium was found actively developing onboth rusts by the latter part of October. All blister rustcankers inoculated in 1932 were dead by July, 1933.There is no doubt about the effectiveness of F. bac-tridioides in destroying Cronartium ribicola once it at-tacks the cankers.

H. W. WOLLENWEBERBERLIN-DAHLEM

BOOKS RECEIVEDCOKER, WILLIAm C. and HENRY R. ToTrN. Trees of the

Southeastern States. Pp. 399. Illustrated. Univer-sity of North Carolina Press. $2.00.

MACBRIDE, THOMAS H. and G. W. MARTiN. The Myxo-mnycetes. Pp. 338. 21 plates. Macmillan. $6.00.

NOWLAN, FaRIalCK S. Analytic Geonsetry. Second edi-tion. Pp. xii + 352. Illustrated. McGraw-Hill. $2.25.

572 SCIENCE

Page 2: SCIENCEGoodding, cooperating with the Oregon Experiment Station, Corvallis, a mummiedcone of the Chihuahua pine (Pinus leiophylla Sehlechtendahl and Chamisso) from the Chiricahua Mountains

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Page 3: SCIENCEGoodding, cooperating with the Oregon Experiment Station, Corvallis, a mummiedcone of the Chihuahua pine (Pinus leiophylla Sehlechtendahl and Chamisso) from the Chiricahua Mountains

10 SCIENCE-ADVERTISEMENTS VoL. 79, No. 2060

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10 SCIENCE-ADVERTISEMENTS VOL. 79, NO. 2060