RAPID SALMONELLA TESTING

Solus Salmonella ELISAAssay and Automated Workflow Package

2

EFFICIENT SALMONELLA TESTING – RESULTS AVAILABLE IN 39 HOURS

Rapid, accurate analysis for food and environmental samples

MULTIPLE MATRICES CAN BE TESTED USING A SINGLE ASSAY

Efficiency Made Simple

In a fast-paced food testing lab like yours, it’s crucial to have easy-to-use, cost-effective, efficient methods for detecting Salmonella in food and environmental samples.

Our Solus Salmonella ELISA kit is versatile and can be used manually or in conjunction with automation. It’s robust, reliable and certified by AOAC Performance Tested Method program and NF VALIDATION by AFNOR Certification to ISO 16140.

Our test system is perfect for labs looking to achieve higher sample throughput, improved laboratory workflow, reduced technician time, and increased capacity for testing.

Rapid Results

• Results available in 39 hours

• Two hours to presumptive negative/positive results post selective enrichment

Cost Effectiveness

• Increased laboratory efficiency

• Reduced demand on resources

• Reduced waste

Efficiency

• High efficiency when automated

• Significantly reduced technician time

Versatile

• Available in 96- or 480-well formats

Reliable

• Independent validation demonstrates high levels of sensitivity and specificity

Solus Salmonella ELISA Benefits

Solus Salmonella ELISA

3

HIGH THROUGHPUT – INCREASING EFFICIENCY

Test Methodology

STEP 1Add sample to BPW and mix

Incubation: 37°C, 16-20 hrs.

STEP 2Transfer 0.1 ml culture to RVS

Incubation: 41.5°C, 21-27 hrs.

STEP 3Transfer 1 ml culture

to boiling tube

Boil: 85-100°C, 15-20 min.

STEP 4Pipette 100 µl sample

into wells (plus controls)

Incubation:37°C, 30 min.

STEP 5Wash plate and add 100 µl of conjugate

Incubation: 37°C, 30 min.

STEP 6Wash plate and add 100 µl of substrate

Incubation: 20-25°C, 30 min.

Solus Salmonella ELISA Workflow

Solus Salmonella ELISA automated process is carried out on a Dynex DS2 instrument. A single DS2 processes two 96-well plates simultaneously, generating results for up to 558 samples in an 8-hour shift.

Once the instrument is loaded, the technician can walk away for up to 2 hours during each run, providing time to carry out other essential laboratory tasks. Dynex DS2

1

2

3

4

5

6

7

8

9

10

11

12

A B C D E F G H

Solus Automated Process

1

2

3

4

5

6

7

8

9

10

11

12

A B C D E F G H

1

2

3

4

5

6

7

8

9

10

11

12

A B C D E F G H

0,1 mlSample

1

2

3

4

5

6

7

8

9

10

11

12

A B C D E F G H

THE SYSTEM IS EASY TO USE AND TO IMPLEMENT

STEP 7Add 100 µl of stop solution

Read plate: 450 nm

Ordering information

Product Code Product Description Pack Size

SAL-0096S Solus Salmonella ELISA 96 wells

SAL-0480S Solus Salmonella ELISA 480 wells

RVS001 Solus RVS Powder 500 g

MED017-10 kg Buffered Peptone Water 10 kg

DS2000 DS2 Automation 1

PERFORMANCE TESTED

RESEARCH INSTITUTEAOAC

LICENSE NUMBER 051601

Validated to ISO 16140 and approved for use by AFNOR. Certificate number SOL 37/01-06/13.

Certified by the AOAC Performance Tested Method (PTM) program certificate number 051601 for the detection of Salmonella.

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

+

B

_

Negative Control Blank Well Positive (After Substrate Addition)Positive Control Sample wells Positive (After Stop Solution Addition)

Sample

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright ©2020, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 74747 PKI

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

For sales and support contacts, please visit www.solusscientific.com

Workflow Comparison: Solus Salmonella vs ISO 6579

Timescale Day 0 Day 1 Day 2 Day 3

ISO 6579

Solus Salmonella 25 g food in 225 ml BPW

0.1 ml BPW in to 10 ml RVS

Take 1-2 ml heat kill, and test

Read plates 0.1 ml BPW in to 10 ml mKTTn

Streak on to XLD + BGA

25 g food in 225 ml BPW

Incubate 37 °C 16-20 hrs.

Incubate 41.5 °C 21-27 hrs.

Incubate 37 °C 21-27 hrs.

0.1 ml BPW in to 10 ml RVS

Incubate 37 °C 21-27 hrs.

Incubate 37 °C 16-20 hrs.

Incubate 41.5 °C 21-27 hrs.

+ve result confirm

Read plates

+ve result confirm

Streak on to XLD + BGA

Incubate 37 °C 21-27 hrs.

Report -ve

+ve result confirm

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

+

B

_

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

+

B

_

Solu

s Au

tom

ated Pro

cess

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

+B _

Solu

s Au

tom

ated Pro

cess

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 6 7 8 9 10 11 12

+B _

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solu

s Au

tom

ated Pro

cess

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solu

s Au

tom

ated Pro

cess

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solus Auto

mated Proce

ss

1 2

3

4

5 6

7

8

9 10 1

1 12

100 ml

Solus Automated Process

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solu

s Au

tom

ated Pro

cess

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Solu

s Au

tom

ated Pro

cess

1 2 3 4 5 6 7 8 9 10 11 12

100 ml

Report -ve

Report -ve