purosynth™ resins for solid phase peptide synthesis6c02cac8-fd4a-41e6... · solid phase peptide...

Purosynth™Resins for Solid Phase Peptide Synthesis

Since 1981, Purolite® has grown into the world’s premier resin-based separation, purification and extraction technology manufacturer and innovation leader, with manufacturing facilities, advanced research laboratories and over 1400 people employed world-wide.

Your solutions company

Purolite® Life Sciences focuses on any applications involving interactions with people, bringing innovative thinking and a distinguished history of resin technology expertise to the global Life Sciences marketplace.

APIs, enzyme carriers, immobilized enzymes, and agarose or synthetic chromatography resins for purification and separation, to support research and development and production-scale applications in pharmaceuticals, protein purification, food processing, bioprocessing and fine chemical markets.

“We provide solutions for our customers’ most critical questions.”

QualityPurolite® maintains a global Quality Management System (QMS) which supports BSI requirements of ISO 9001. Compliance is monitored and maintained through a quality assurance and regulatory team, who conduct internal audits to ensure operations meet the guidelines and protocols for equipment and procedures. Our teams are given continuous training on quality processes to ensure batch-to-batch consistency, and the highest product quality.

Secure Supply through Manufacturing ExcellenceEnsuring reliable availability of our resins is vital to customers, and of paramount importance to Purolite® Life Sciences. As a leading supplier of resin to the world’s most regulated industries, we recognize that our resins are critical purification products. As such, a real-world security-of-supply system is in place to support your process requirements for business continuity.

Supply risk is managed end-to-end, with a global network of qualified suppliers. Long-term supply agreements with periodic audits ensure consistency and ‘fit for purpose’ performance.

Purolite® has manufacturing facilities at 4 strategic locations in the USA, Asia and Europe, including the recent addition of one of the world’s largest agarose resin manufacturing facilities located in Llantrisant, South Wales, UK.

Our agarose manufacturing facility includes state-of-the-art Siemens® automation systems and can securely supply 30% of the current annual global demand for agarose chromatography resins to the biopharmaceutical sector.

Our Romanian facility is FDA-inspected, with a total of 4 state-of-the-art clean rooms. These offer separate facilities for ligand/enzyme immobilization, removal of fines, solvent or purified water washing, screening, vacuum drying and packaging. LifetechTM and Chromalite® products are manufactured in a clean room dedicated solely to Life Sciences products.

100% focused on resin technology.

Global manufacturing at facilities in the UK, Romania, China and USA.

De-risked long-term supplythrough dual-sourcing.

25+ years of regulatory experiencefrom FDA inspected cGMP facility.

Over 35+ years of experience in solving advanced R&D and purification challenges.

PuroSynth™

PuroSynth™ supports for Solid Phase Peptide Synthesis (SPPS) are made of polystyrene crosslinked with 1% DVB. They are characterized by uniform particle size distribution, high swelling and superior peptide synthesis

performance

Safety data

Purolite® Life Sciences maintains Material Safety DataSheets (MSDS for the U.S. and ERSDS for Europe) onall of its bulk resins. These data sheets containrelevant information that you may need to protectyour customers and employees against any knownhealth or safety hazards associated with our products.

Purolite® Life Sciences supplies copies of our Material Safety Data Sheets with all bulk resins. These describe precautions to be taken in the storage and handling of our products and in the maintenance of the health and safety of persons exposed to our products, the public and the environment with respect to our products.

Your trusted partners for resin technology solutions.

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Purolite® continues to lead the future of chromatography with the expertly designed range of PuroSynth™ solid phase peptide synthesis resins.

Polystyrene is the most common core resin used in solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic solvents, and of course cost and availability.

PuroSynth™ supports for Solid Phase Peptide Synthesis (SPPS) are made of polystyrene containing 1% divinylbenzene (DVB) as a crosslinking agent. This polystyrene resin is insoluble in all common solvents, it swells well in most organic solvents and is supplied as uniform spherical beads.

Background

Features of Purosynth™ Resins

Complete range of products for Fmoc1 and

Boc2 chemistry

Purosynth™

Made in the EU in an ISO 9001:2008 and ISO

18001:2007certified facility

Controlled residual organics for higher purity

Narrow particle size distribution

(manufactured by jetting)

Large scale batches (500+ Kg per batch)

10

Product Line

As shown in both Figure 1 and Table 1, PuroSynth™ resins for Solid Phase Peptide Synthesis are offered with 5 different functionalities:

CH2Cl

CH2NH2

CH2O CH2OH

CH

NH2

CH3

·HCl

Cl

Cl

4-Alkoxybenzyl Alcohol ResinPuroSynthTM Wang

Aminomethyl ResinPuroSynthTM AM

4-Methylbenzhydrylamine Hydrochloride Salt ResinPuroSynthTM MBHA

Chloromethyl ResinPuroSynthTM CM

2-Chlorotrityl Chloride ResinPuroSynthTM CTC

PuroSynthTM

As shown in both Figure 1 and Table 1, PuroSynth™ resins for Solid Phase Peptide Synthesis are offered with 5 different functionalities:

• PuroSynth™ CM• PuroSynth™ Wang• PuroSynth™ CTC• PuroSynth™ MBHA• PuroSynth™ AM

Figure 1. Chemical structures of PuroSynth™ product range

06 PuroSynth™ - Product Line

PuroSynthTM - Product Line

Figure 1: Chemical structures of Purosynth™ product range

Product Functional Group Particle Size Micron (USA Mesh) a

Loading (mmol/g) Description

PuroSynth™ CM Chloromethyl Resin 75-150 (100 - 200)

0.4 - 0.80.8 - 1.21.2 - 1.6

PuroSynth™ (Merrifield resin) is the standard support for the acid peptide synthesis by Boc strategy.

PuroSynth™ Wang4-AlkoxybenzylAlcohol Resin(Wang linker)

75-150 (100 - 200)

0.4 - 0.80.8 - 1.2

PuroSynth™ Wang (4-Alkoxybenzyl alcohol resin) is the standard support for acid peptide synthesis by Fmoc strategy.

PuroSynth™ CTC 2-Chlorotrityl Chloride Resin (2-CTC Resin)

75-150 (100 - 200)

0.4 - 0.80.8 - 1.2

PuroSynth™ CTC (2-chlorotrityl chloride resin) is a resin which is particularly useful for the preparation of protected peptides by Fmoc strategy.

PuroSynth™ MBHA 4-Methylbenzh-drylamine Hydrochloride Salt Resin (MBHA Resin)

75-150 (100 - 200)

0.4 - 0.80.8 - 1.2

PuroSynth™ MBHA (4-methylbenzhydrylamine resin) is used in the synthesis of amide peptides by Boc strategy

PuroSynth™ AM Aminomethyl Resin 75-150 (100 - 200)

0.4 - 0.80.8 - 1.2

PuroSynth™ AM (aminomethyl resin) is the core resin to which various linkers could be attached through a stable amide bond.

PuroSynth™ Rink Amide AM

4-(2',4'-Dimethoxyphenyl-Fmoc-aminomethyl)-phenoxy resin

75-150(100-200)

0.4 - 0.80.8 - 1.2

PuroSynth™ Rink Amide AM/S resin is optimal for Fmoc Solid Phase Synthesis of Peptides. It is formed by coupling the rink amide linker to aminomethylpolystyrene and is ideal for use in the synthesis of long and difficult amide peptides

a S grade : 75 -150 micron (100-200 mesh); F grade : 40 - 75 micron (200-400 mesh) available on request.

Table 1. Key Features of PuroSynth™ product range

Descriptions CharacteristicsResin Polystyrene

Cross-linking 1% Divinylbenzene (DVB)

Supplied as Dry

Loss on drying (%) ≤2

Chemical resistance Stable to all organic solvents and chemicals

Temperature resistance Up to 140°C

Swelling (ml/g) Up to 9ml/g

Patricle size range (micron) 75 - 150 (S grade) 40 - 75 (F grade)

Particle size range (mesh) 100 - 200 (S grade) 200 - 400 (F grade)

Uniformity coefficient (UC)1 <1.3

Typical loading (mmol/g) 0.4 - 0.8, 0.8 -1.2, 1.2 - 1.6

Optimal storage conditions2 2 - 20°C

Packaging Heat-seal, PP bottleAluminium bags in kegs

Typical industrial batch 100+ Kg per batch

Typical packaging options 25g, 100g, 1Kg, 5Kg and 20Kg

Expiry date 1 year

1The uniformity coefficient is defined as: UC = d60/ d10. This coefficient measures the "width" of the distribution, and is reflected in the width of the

Gaussian bell curve. If all beads had the same size, it would be equal to 1.0.2 Optimal storage condition for PuroSynth CM/S is 2-8 °C

Table 2: General feature of PuroSynth™ product range

"Polystyrene is the most common core resin used in solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic solvents, cost and availability."

18

PuroSynth™ has a complete range of products for Fmoc1 and Boc2 chemistry. Controlled residual organics in the resins lead to higher purity of final peptide

Swelling Properties of PuroSynth™ Resins

Even though the cross-linked polystyrene of PuroSynth™ resin is insoluble in organic solvents, it swells in aprotic solvents such as dimethylformamide (DMF), dichloromethane (DCM), acetonitrile (ACN) and the green solvents 2-methyltetrahydrofuran (MeTHF) andγ-valerolactone (GVL) as shown in Figure 2.

The advantage of having a 1% DVB cross-linked resin is that it results in a significant increase in swelling, compared to a higher cross-linked resin as a polystyrene with 2% DVB.

Resin swelling is a key factor in solid phase peptide synthesis, since reaction kinetics are diffusion controlled. Consequently, a resin that swells more will have a higher diffusion rate of reagents into the core of the matrix, resulting in shorter reaction times and more complete chemical conversions.

The good solvent properties of PuroSynth™ resins in green solvents, such as MeTHF and biomass derived GVL open up the possibility to use these resins on green chemistry processes for green solid phase peptide synthesis.

Figure 2: The swelling properties of PuroSynth™

0

1

2

3

4

5

6

DMF DCM ACN MeTHF GVL

Sw

ellin

g (m

l/g)

PuroSynth Competitor resin 1 Competitor resin 2

Even though the cross-linked polystyrene of PuroSynth™ resin

is insoluble in organic solvents, it swells in aprotic solvents

such as dimethylformamide (DMF), dichloromethane (DCM),

acetonitrile (ACN) and the green solvents

2-methyltetrahydrofuran (MeTHF) and γ-valerolactone (GVL) as

shown in Figure 2.

The advantage of having a 1% DVB cross-linked resin is that

it results in a significant increase in swelling, compared to a

higher cross-linked resin as a polystyrene with 2% DVB.

Resin swelling is a key factor in solid phase peptide synthesis,

since reaction kinetics are diffusion controlled. Consequently,

a resin that swells more will have a higher diffusion rate of

reagents into the core of the matrix, resulting in shorter

reaction times and more complete chemical conversions.

The good solvent properties of PuroSynth™ resins in green

solvents, such as MeTHF and biomass derived GVL open up the

possibility to use these resins on green chemistry processes

for green solid phase peptide synthesis.

Figure 2. The swelling properties of PuroSynth™

09Swelling Properties of PuroSynth™ Resins

Swelling Properties of PuroSynthTM Resins

17

0606

One of the key features of PuroSynth™ products is narrow particle size distribution. ‘Jetting’ - the Purolite® patented manufacturing process, allows the production of uniform particle size, with low uniformity coefficient and high productivity in a continuous mode. This enables a more economic andenvironmentally friendly (reduced waste) process, than classic batch emulsion polymerisation.

The narrow particle size distribution obtained by Jetting is unique to PuroSynthTM resins for SPPS.In SPPS, the uniformity of particle size offers great advantages in terms of uniform kinetics and swelling during peptide synthesis, and so ensures better purity of the final peptide.

Jetting technology allows for the production of large batches (500+ kg) in a very short time

Particle Size Distribution

PuroSynth™ products are manufactured usingmembrane or‘can’ jetting (Figure 3), which uses a very small membrane, through which the polymer is passed. This device is capable of producing uniform particle size resins from ~15 to 250 micron.

PuroSynth™ products have unique performance characteristics. These include a narrow particle size distribution (UC <1.3), a high yield and economical process.

The Jetting Process

Patented, Innovative Manufacturing

Monomer Phase

Aqueous Phase

Pump

Pump

Shaker

oscillations

Organic Phase in membrane can

Continuous Phase

Droplets

Drops formed

Jet

Monomer Feed

Membrane

vibration

Collection Vessel

Polymerization

Heat Exchanger / Condenser

Figure 3: Jetting technology through a membrane for the manufacture of Purosynth™ products

The result of this jetting process is a narrow gaussiandistribution of uniform spherical beads. Figure 4 shows an example of a particle size distribution of PuroSynth™ products. Figure 5 shows an electron microscopy picture for PuroSynth™ products.

Figure 4: Particle size distribution for a batch of PuroSynth™ CTC/S with UC 1.2

Figure 5: Electron microscopy images for Purosynth™ Wang/S

Standard SPPS conditions used are Fmoc/butyl chemistry and DIC-OxymaPure for coupling; 20% piperidine/DMF for Fmoc removal in DMF as a solvent.

Results show that PuroSynth™ resins are efficient in the synthesis of peptides ranging from 5-mer (Figure 6) up to 28-mer (Figure 10). In all cases PuroSynth™ functionalized resins provide excellent purity (measured by HPLC) and recovery of final peptide (determined by weight increase) also, when compared to commercially available products.

Applications

PuroSynth™ resins have been tested by Prof. Fernando Albericio in the University of KwaZulu-Natal, School of Chemistry, Durban (South Africa) and in the University of Barcelona, Department of Organic Chemistry, Barcelona (Spain).

Performances have been evaluated in terms of the purity of the final product and recovery. Synthesis of model peptides has been carried out in both automatic assisted heating microwave systems and in manual mode.

Synthesis of a Pentapeptide (5 amino acids)

12 PuroSynth™ - Applications

PuroSynth™ resins have been tested by Prof. Fernando

Albericio in the University of KwaZulu-Natal, School of

Chemistry, Durban (South Africa) and in the University of

Barcelona, Department of Organic Chemistry, Barcelona

(Spain).

Performances have been evaluated in terms of the purity of the

final product and recovery. Synthesis of model peptides has

been carried out in both automatic assisted heating microwave

systems and in manual mode.

Standard SPPS conditions used are Fmoc/tButyl chemistry

and DIC-OxymaPure for coupling; 20% piperidine/DMF for

Fmoc removal in DMF as a solvent.

Results show that PuroSynth™ resins are efficient in the

synthesis of peptides ranging from 5-mer (Figure 6) up to

28-mer (Figure 10). In all cases PuroSynth™ functionalized

resins provide excellent purity (measured by HPLC) and

recovery of final peptide (determined by weight increase) also,

when compared to commercially available products.

Synthesis of an octapeptide (8 amino acids)

Synthesis of a pentapeptide (5 amino acids)

NH2

O

HO

NH

H

O

HN

H

O

NH O

HN

OH

O

Chemical Formula: C28H37N5O7Exact Mass: 555,27

Molecular Weight: 555,62

H-Tyr-Gly-Gly-Phe-Leu-OH

H-Gly-Pro-Glu-Thr-Ala-Phe-Leu-Lys-OH

NH2O

N

O

HN

O

OHO

NH O

OHHN

O

NH O

HN

O

NH O

OH



NH2

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

Figure 6. Synthesis of 5-mer peptide on PuroSynth™ CTC/S.

PuroSynthTM CTC/S

The above acid octapeptide was synthetised with similar purity and

recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S

resins. The amide version of this octapeptide was successfully

synthetised using the PuroSynth™ AM/S containing the Rink linker resin.

0.80

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

AU

0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00

Minutes

PuroSynthTM CTC/S


PuroSynthTM - Applications

Figure 6: Synthesis of 5-mer peptide on PuroSynth™ CTC/S






(Spain).
















NH2

O

HO

NH

H

O

HN

H

O

NH O

HN

OH

O





NH2O

N

O

HN

O

OHO

NH O

OHHN

O

NH O

HN

O

NH O

OH



NH2

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600


PuroSynthTM CTC/S





0.80

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

AU

0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00

Minutes

PuroSynthTM CTC/S



Synthesis of a Octapeptide (8 amino acids)






(Spain).
















NH2

O

HO

NH

H

O

HN

H

O

NH O

HN

OH

O





NH2O

N

O

HN

O

OHO

NH O

OHHN

O

NH O

HN

O

NH O

OH



NH2

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600


PuroSynthTM CTC/S





0.80

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

AU

0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00

Minutes

PuroSynthTM CTC/S









(Spain).
















NH2

O

HO

NH

H

O

HN

H

O

NH O

HN

OH

O





NH2O

N

O

HN

O

OHO

NH O

OHHN

O

NH O

HN

O

NH O

OH



NH2

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600


PuroSynthTM CTC/S





0.80

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

AU

0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00

Minutes

PuroSynthTM CTC/S



The previous acid octapeptide was synthetised with similar purity and recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S resins.

The amide version of this octapeptide wassuccessfully synthetised using the PuroSynth™ AM/S containing the Rink linker resin.

Synthesis of ACP(65-74) peptide (10 amino acids)


H2N CHCCH

O

CH3CH3

HN CHC

CH2

O

CH2CNH2

O

HN CHC

CH3

O HN CHC

CH3

O HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

COH

O

HN CHC

CH2

O

OH

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

CNH2

O

HN CHC

HOH

O



ACP(65-74) is the current model peptide

used to set up synthetic strategies.

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

700

800

min0 2 4 6 8 10 12 14

Norm.

-50

0

50

100

150

200

H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH

PuroSynthTM AM/Sfunctionalized with Rink linker

Competitor Resin functionalized with Rink linker

Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.

Synthesis of Jung-Redemann decapeptide (10 amino acids)

H2N CHCCH2

O

HN

HN CHC

CH2

O HN CHC

CH

O

OHCH3

HN CHC

CH

O

OHCH3

HN CHC

CH2

O

CHCH3CH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

OH

HN CHC

CH

O

OHCH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

OHO

CH2SCH3

Chemical Formula: C58H89N11O15SExact Mass: 1211,63


Jung-Redemann decapeptide (JR) is the

C-terminal part of a 26-mer peptide,

considered a difficult sequence, more

difficult to manufacture than ACP.

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH

PuroSynthTM AM/S Competitor AM Resin

Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin

13PuroSynth™ - Applications

ACP(65-74) is the current model peptide used to set up synthetic strategies.


H2N CHCCH

O

CH3CH3

HN CHC

CH2

O

CH2CNH2

O

HN CHC

CH3

O HN CHC

CH3

O HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

COH

O

HN CHC

CH2

O

OH

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

CNH2

O

HN CHC

HOH

O





min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

700

800

min0 2 4 6 8 10 12 14

Norm.

-50

0

50

100

150

200






H2N CHCCH2

O

HN

HN CHC

CH2

O HN CHC

CH

O

OHCH3

HN CHC

CH

O

OHCH3

HN CHC

CH2

O

CHCH3CH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

OH

HN CHC

CH

O

OHCH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

OHO

CH2SCH3







min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600






H2N CHCCH

O

CH3CH3

HN CHC

CH2

O

CH2CNH2

O

HN CHC

CH3

O HN CHC

CH3

O HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

COH

O

HN CHC

CH2

O

OH

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

CNH2

O

HN CHC

HOH

O





min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

700

800

min0 2 4 6 8 10 12 14

Norm.

-50

0

50

100

150

200






H2N CHCCH2

O

HN

HN CHC

CH2

O HN CHC

CH

O

OHCH3

HN CHC

CH

O

OHCH3

HN CHC

CH2

O

CHCH3CH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

OH

HN CHC

CH

O

OHCH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

OHO

CH2SCH3







min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600





Figure 8: The synthesis of 10-mer peptide on Purosynth™ AM/S & Comparison with compettor AM resin



H2N CHCCH

O

CH3CH3

HN CHC

CH2

O

CH2CNH2

O

HN CHC

CH3

O HN CHC

CH3

O HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

COH

O

HN CHC

CH2

O

OH

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

CNH2

O

HN CHC

HOH

O





min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

700

800

min0 2 4 6 8 10 12 14

Norm.

-50

0

50

100

150

200






H2N CHCCH2

O

HN

HN CHC

CH2

O HN CHC

CH

O

OHCH3

HN CHC

CH

O

OHCH3

HN CHC

CH2

O

CHCH3CH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

OH

HN CHC

CH

O

OHCH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

OHO

CH2SCH3







min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600





Jung-Redemann decapeptide (JR) is theC-terminal part of a 26-mer peptide, considered a difficult sequence, more difficult to manufacture than ACP.


H2N CHCCH

O

CH3CH3

HN CHC

CH2

O

CH2CNH2

O

HN CHC

CH3

O HN CHC

CH3

O HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

COH

O

HN CHC

CH2

O

OH

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

CNH2

O

HN CHC

HOH

O





min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

700

800

min0 2 4 6 8 10 12 14

Norm.

-50

0

50

100

150

200






H2N CHCCH2

O

HN

HN CHC

CH2

O HN CHC

CH

O

OHCH3

HN CHC

CH

O

OHCH3

HN CHC

CH2

O

CHCH3CH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

OH

HN CHC

CH

O

OHCH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

OHO

CH2SCH3







min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600






H2N CHCCH

O

CH3CH3

HN CHC

CH2

O

CH2CNH2

O

HN CHC

CH3

O HN CHC

CH3

O HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

COH

O

HN CHC

CH2

O

OH

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

CNH2

O

HN CHC

HOH

O





min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600

700

800

min0 2 4 6 8 10 12 14

Norm.

-50

0

50

100

150

200






H2N CHCCH2

O

HN

HN CHC

CH2

O HN CHC

CH

O

OHCH3

HN CHC

CH

O

OHCH3

HN CHC

CH2

O

CHCH3CH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

O

OH

HN CHC

CH

O

OHCH3

HN CHC

CH

O

CH3CH2CH3

HN CHC

CH2

OHO

CH2SCH3







min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

min0 2 4 6 8 10 12 14

Norm.

0

100

200

300

400

500

600





Figure 8: The synthesis of 10-mer peptide on Purosynth™ AM/S & Comparison with compettor AM resin

Synthesis of Thymosin peptide (28 amino acids)

0.80

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

AU

0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00

Minutes

14 PuroSynth™ - Applications and Regulatory


N

OO N

O

OON

ON

ON

ON

O

OON

O

ON

O

ON

O

ON

O

O O

NO

NO

ON

O

ON

O

N

NO

OON

ON

O

N

NO

O O

NO

N

NO

N

NO

OO

NO

NO

NO

O O

NO

OO

NO

NO

OO

N NO

NO



Thymosin is a commercial product on the market.

H-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-NH2

PuroSynthTM AM/S

Figure 10. Synthesis of 28-mer peptide on PuroSynth™ AM/S.

PuroSynthTM - Regulatory

All PuroSynth™ products are made in Europe under an ISO 9001:2008 and ISO 18001:2007 certified facility.

0.80

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

AU

0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00

Minutes

14 PuroSynth™ - Applications and Regulatory


N

OO N

O

OON

ON

ON

ON

O

OON

O

ON

O

ON

O

ON

O

O O

NO

NO

ON

O

ON

O

N

NO

OON

ON

O

N

NO

O O

NO

N

NO

N

NO

OO

NO

NO

NO

O O

NO

OO

NO

NO

OO

N NO

NO



Thymosin is a commercial product on the market.

H-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-NH2

PuroSynthTM AM/S

Figure 10. Synthesis of 28-mer peptide on PuroSynth™ AM/S.

PuroSynthTM - Regulatory

All PuroSynth™ products are made in Europe under an ISO 9001:2008 and ISO 18001:2007 certified facility.

Figure 10: Synthesis of 28-mer peptide on Purosynth™ AM/S

PuroSynth™ Regulatory

All PuroSynth™ products are made in Europe under an ISO 9001: 2015 and ISO 18001:2007 certified facility.

References

O. Al Musaimi, Y. E. Jad, A. Kumar, J. M. Collins, A. Basso, B. G. de la Torre, F. Albericio, Investigating green ethers for the precipitation of peptides after global deprotection in solid-phase peptide synthesis, Curr. Opin. Green Sustain. Chem. 11 (2018) 99-103, 10.1016/j.cogsc.2018.06.017.

O. Al Musaimi, Y. E. Jad, A. Kumar, A. El-Faham, J. M. Collins, A. Basso, B. G. d. l. Torre, F. Albericio, Greening the Solid-Phase Peptide Synthesis Process. 2-MeTHF for the Incorporation of the First Amino Acid and Precipitation of Peptides after Global Deprotection, Org. Process Res. Dev. (2018), 10.1021/acs.oprd.8b00335.

O. Al Musaimi, S. Serban, Y. E. Jad, Z. Ma, A. Kumar, C. Ji, B. G. d. l. Torre, Nathan R. East, A. Basso, F. Albericio, New Manufacturing Process to produce Highly Uniform Resins with Excellent Performances in solid phase peptide synthesis, Chimica Oggi/Chemistry Today 37(2), 2019, 8-10.

O. Al Musaimi, S. Serban, Y. E. Jad, Z. Ma, A. Kumar, C. Ji, B. G. d. l. Torre, A. Basso, F. Albericio, Jetting manu-facturing of resins for solid-phase peptide synthesis, Chimica Oggi/Chemistry Today 37(1), 2019, 20-23, https://www.teknoscienze.com/tks_article/jetting-manufacturing-of-resins-for-solid-phase-peptide-synthesis/

Al Musaimi, O.; El-Faham, A.; Almarhoon, Z.; Basso, A.; de la Torre, B.G.; Albericio, F. Bypassing Osmotic Shock Dilemma in a Polystyrene Resin Using the Green Solvent Cyclopentyl methyl Ether (CPME): A Morphological Perspective. Polymers 2019, 11, 874.

Al Musaimi, O.; El-Faham, A.; Basso, A.; de la Torre, B.G.; Albericio, F. γ-valerolactone (gvl): An eco-friendly an-choring solvent for solid-phase peptide synthesis. Tetrahedron Letters 2019. 10.1016/j.tetlet.2019.151058

Placing your order

Purosynth™ Wang/S Ordering Information

BULK RESIN LOADING (mmol/g)

Purosynth™ Wang/S

Purosynth™ Wang/S

Purosynth™ Wang/F

Purosynth™ Wang/F

25g

100g

1kg

5kg

20kg

-163

-164

-144

-343

-700

LSPS02401

LSPS02421

LSPS02400

LSPS02420

0.4 - 0.8

0.8 - 1.2

0.4 - 0.8

0.8 - 1.4

PACK SIZE CODE SUFFIX

ORDER NUMBER

Purosynth™ CTC/S Ordering Information


Purosynth™ CTC/S

Purosynth™ CTC/S

Purosynth™ CTC/F

Purosynth™ CTC/F

25g

100g

1kg

5kg

20kg

-163

-164

-144

-343

-700

LSPS03401

LSPS03421

LSPS03400

LSPS03420

0.4 - 0.8

0.8 - 1.2

0.4 - 0.8

0.8 - 1.2


ORDER NUMBER

Purosynth™ AM/S Ordering Information


Purosynth™ AM/S

Purosynth™ AM/S

Purosynth™ AM/F

Purosynth™ AM/F

25g

100g

1kg

5kg

20kg

-163

-164

-144

-343

-700

LSPS04401

LSPS04421

LSPS04400

LSPS04420

0.4 - 0.8

0.8 - 1.2

0.4 - 1.8

0.8 - 1.2


ORDER NUMBER

Purosynth™ MHBA/S Ordering Information


Purosynth™ MHBA/S

Purosynth™ MHBA/S

Purosynth™ MHBA/F

Purosynth™ MHBA/F

25g

100g

1kg

5kg

20kg

-163

-164

-144

-343

-700

LSPS05401

LSPS05421

LSPS05400

LSPS05420

0.4 - 0.8

0.8 - 1.2

0.4 - 0.8

0.8 - 1.2


ORDER NUMBER

Purosynth™ CM/S Ordering Information


Purosynth™ CM/S

Purosynth™ CM/S

Purosynth™ CM/S

Purosynth™ CM/F

Purosynth™ CM/F

Purosynth™ CM/F

25g

100g

1kg

5kg

20kg

-163

-164

-144

-343

-700

LSPS01401

LSPS01421

LSPS01441

LSPS01400

LSPS01420

LSPS01440

0.4 - 0.8

0.8 - 1.2

1.2 - 1.6

0.4 - 0.8

0.8 - 1.2

1.2 - 1.6


ORDER NUMBER

Purosynth™ Rink Amide AM/S Ordering Information


Purosynth™ Rink Amide AM/S

Purosynth™ Rink Amide AM/S

Purosynth™ Rink Amide AM/F

Purosynth™ Rink Amide AM/F

25g

100g

1kg

5kg

20kg

-163

-164

-144

-343

-700

LSPS06401

LSPS06421

LSPS06400

LSPS06420

0.4 - 0.8

0.8 - 1.2

0.4 - 0.8

0.8 - 1.2


ORDER NUMBER

Contact information

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