![Page 1: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/1.jpg)
Purosynth™Resins for Solid Phase Peptide Synthesis
![Page 2: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/2.jpg)
Since 1981, Purolite® has grown into the world’s premier resin-based separation, purification and extraction technology manufacturer and innovation leader, with manufacturing facilities, advanced research laboratories and over 1400 people employed world-wide.
![Page 3: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/3.jpg)
Your solutions company
Purolite® Life Sciences focuses on any applications involving interactions with people, bringing innovative thinking and a distinguished history of resin technology expertise to the global Life Sciences marketplace.
APIs, enzyme carriers, immobilized enzymes, and agarose or synthetic chromatography resins for purification and separation, to support research and development and production-scale applications in pharmaceuticals, protein purification, food processing, bioprocessing and fine chemical markets.
“We provide solutions for our customers’ most critical questions.”
![Page 4: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/4.jpg)
QualityPurolite® maintains a global Quality Management System (QMS) which supports BSI requirements of ISO 9001. Compliance is monitored and maintained through a quality assurance and regulatory team, who conduct internal audits to ensure operations meet the guidelines and protocols for equipment and procedures. Our teams are given continuous training on quality processes to ensure batch-to-batch consistency, and the highest product quality.
Secure Supply through Manufacturing ExcellenceEnsuring reliable availability of our resins is vital to customers, and of paramount importance to Purolite® Life Sciences. As a leading supplier of resin to the world’s most regulated industries, we recognize that our resins are critical purification products. As such, a real-world security-of-supply system is in place to support your process requirements for business continuity.
Supply risk is managed end-to-end, with a global network of qualified suppliers. Long-term supply agreements with periodic audits ensure consistency and ‘fit for purpose’ performance.
Purolite® has manufacturing facilities at 4 strategic locations in the USA, Asia and Europe, including the recent addition of one of the world’s largest agarose resin manufacturing facilities located in Llantrisant, South Wales, UK.
Our agarose manufacturing facility includes state-of-the-art Siemens® automation systems and can securely supply 30% of the current annual global demand for agarose chromatography resins to the biopharmaceutical sector.
Our Romanian facility is FDA-inspected, with a total of 4 state-of-the-art clean rooms. These offer separate facilities for ligand/enzyme immobilization, removal of fines, solvent or purified water washing, screening, vacuum drying and packaging. LifetechTM and Chromalite® products are manufactured in a clean room dedicated solely to Life Sciences products.
![Page 5: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/5.jpg)
100% focused on resin technology.
Global manufacturing at facilities in the UK, Romania, China and USA.
De-risked long-term supplythrough dual-sourcing.
25+ years of regulatory experiencefrom FDA inspected cGMP facility.
Over 35+ years of experience in solving advanced R&D and purification challenges.
![Page 6: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/6.jpg)
PuroSynth™
PuroSynth™ supports for Solid Phase Peptide Synthesis (SPPS) are made of polystyrene crosslinked with 1% DVB. They are characterized by uniform particle size distribution, high swelling and superior peptide synthesis
performance
![Page 7: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/7.jpg)
![Page 8: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/8.jpg)
Safety data
Purolite® Life Sciences maintains Material Safety DataSheets (MSDS for the U.S. and ERSDS for Europe) onall of its bulk resins. These data sheets containrelevant information that you may need to protectyour customers and employees against any knownhealth or safety hazards associated with our products.
Purolite® Life Sciences supplies copies of our Material Safety Data Sheets with all bulk resins. These describe precautions to be taken in the storage and handling of our products and in the maintenance of the health and safety of persons exposed to our products, the public and the environment with respect to our products.
![Page 9: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/9.jpg)
![Page 10: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/10.jpg)
Your trusted partners for resin technology solutions.
16
![Page 11: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/11.jpg)
17
Purolite® continues to lead the future of chromatography with the expertly designed range of PuroSynth™ solid phase peptide synthesis resins.
![Page 12: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/12.jpg)
Polystyrene is the most common core resin used in solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic solvents, and of course cost and availability.
PuroSynth™ supports for Solid Phase Peptide Synthesis (SPPS) are made of polystyrene containing 1% divinylbenzene (DVB) as a crosslinking agent. This polystyrene resin is insoluble in all common solvents, it swells well in most organic solvents and is supplied as uniform spherical beads.
Background
![Page 13: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/13.jpg)
Features of Purosynth™ Resins
Complete range of products for Fmoc1 and
Boc2 chemistry
Purosynth™
Made in the EU in an ISO 9001:2008 and ISO
18001:2007certified facility
Controlled residual organics for higher purity
Narrow particle size distribution
(manufactured by jetting)
Large scale batches (500+ Kg per batch)
10
![Page 14: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/14.jpg)
Product Line
As shown in both Figure 1 and Table 1, PuroSynth™ resins for Solid Phase Peptide Synthesis are offered with 5 different functionalities:
CH2Cl
CH2NH2
CH2O CH2OH
CH
NH2
CH3
·HCl
Cl
Cl
4-Alkoxybenzyl Alcohol ResinPuroSynthTM Wang
Aminomethyl ResinPuroSynthTM AM
4-Methylbenzhydrylamine Hydrochloride Salt ResinPuroSynthTM MBHA
Chloromethyl ResinPuroSynthTM CM
2-Chlorotrityl Chloride ResinPuroSynthTM CTC
PuroSynthTM
As shown in both Figure 1 and Table 1, PuroSynth™ resins for Solid Phase Peptide Synthesis are offered with 5 different functionalities:
• PuroSynth™ CM• PuroSynth™ Wang• PuroSynth™ CTC• PuroSynth™ MBHA• PuroSynth™ AM
Figure 1. Chemical structures of PuroSynth™ product range
06 PuroSynth™ - Product Line
PuroSynthTM - Product Line
Figure 1: Chemical structures of Purosynth™ product range
![Page 15: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/15.jpg)
Product Functional Group Particle Size Micron (USA Mesh) a
Loading (mmol/g) Description
PuroSynth™ CM Chloromethyl Resin 75-150 (100 - 200)
0.4 - 0.80.8 - 1.21.2 - 1.6
PuroSynth™ (Merrifield resin) is the standard support for the acid peptide synthesis by Boc strategy.
PuroSynth™ Wang4-AlkoxybenzylAlcohol Resin(Wang linker)
75-150 (100 - 200)
0.4 - 0.80.8 - 1.2
PuroSynth™ Wang (4-Alkoxybenzyl alcohol resin) is the standard support for acid peptide synthesis by Fmoc strategy.
PuroSynth™ CTC 2-Chlorotrityl Chloride Resin (2-CTC Resin)
75-150 (100 - 200)
0.4 - 0.80.8 - 1.2
PuroSynth™ CTC (2-chlorotrityl chloride resin) is a resin which is particularly useful for the preparation of protected peptides by Fmoc strategy.
PuroSynth™ MBHA 4-Methylbenzh-drylamine Hydrochloride Salt Resin (MBHA Resin)
75-150 (100 - 200)
0.4 - 0.80.8 - 1.2
PuroSynth™ MBHA (4-methylbenzhydrylamine resin) is used in the synthesis of amide peptides by Boc strategy
PuroSynth™ AM Aminomethyl Resin 75-150 (100 - 200)
0.4 - 0.80.8 - 1.2
PuroSynth™ AM (aminomethyl resin) is the core resin to which various linkers could be attached through a stable amide bond.
PuroSynth™ Rink Amide AM
4-(2',4'-Dimethoxyphenyl-Fmoc-aminomethyl)-phenoxy resin
75-150(100-200)
0.4 - 0.80.8 - 1.2
PuroSynth™ Rink Amide AM/S resin is optimal for Fmoc Solid Phase Synthesis of Peptides. It is formed by coupling the rink amide linker to aminomethylpolystyrene and is ideal for use in the synthesis of long and difficult amide peptides
a S grade : 75 -150 micron (100-200 mesh); F grade : 40 - 75 micron (200-400 mesh) available on request.
Table 1. Key Features of PuroSynth™ product range
![Page 16: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/16.jpg)
Descriptions CharacteristicsResin Polystyrene
Cross-linking 1% Divinylbenzene (DVB)
Supplied as Dry
Loss on drying (%) ≤2
Chemical resistance Stable to all organic solvents and chemicals
Temperature resistance Up to 140°C
Swelling (ml/g) Up to 9ml/g
Patricle size range (micron) 75 - 150 (S grade) 40 - 75 (F grade)
Particle size range (mesh) 100 - 200 (S grade) 200 - 400 (F grade)
Uniformity coefficient (UC)1 <1.3
Typical loading (mmol/g) 0.4 - 0.8, 0.8 -1.2, 1.2 - 1.6
Optimal storage conditions2 2 - 20°C
Packaging Heat-seal, PP bottleAluminium bags in kegs
Typical industrial batch 100+ Kg per batch
Typical packaging options 25g, 100g, 1Kg, 5Kg and 20Kg
Expiry date 1 year
1The uniformity coefficient is defined as: UC = d60/ d10. This coefficient measures the "width" of the distribution, and is reflected in the width of the
Gaussian bell curve. If all beads had the same size, it would be equal to 1.0.2 Optimal storage condition for PuroSynth CM/S is 2-8 °C
Table 2: General feature of PuroSynth™ product range
![Page 17: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/17.jpg)
"Polystyrene is the most common core resin used in solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic solvents, cost and availability."
18
![Page 18: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/18.jpg)
![Page 19: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/19.jpg)
PuroSynth™ has a complete range of products for Fmoc1 and Boc2 chemistry. Controlled residual organics in the resins lead to higher purity of final peptide
![Page 20: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/20.jpg)
Swelling Properties of PuroSynth™ Resins
Even though the cross-linked polystyrene of PuroSynth™ resin is insoluble in organic solvents, it swells in aprotic solvents such as dimethylformamide (DMF), dichloromethane (DCM), acetonitrile (ACN) and the green solvents 2-methyltetrahydrofuran (MeTHF) andγ-valerolactone (GVL) as shown in Figure 2.
The advantage of having a 1% DVB cross-linked resin is that it results in a significant increase in swelling, compared to a higher cross-linked resin as a polystyrene with 2% DVB.
Resin swelling is a key factor in solid phase peptide synthesis, since reaction kinetics are diffusion controlled. Consequently, a resin that swells more will have a higher diffusion rate of reagents into the core of the matrix, resulting in shorter reaction times and more complete chemical conversions.
The good solvent properties of PuroSynth™ resins in green solvents, such as MeTHF and biomass derived GVL open up the possibility to use these resins on green chemistry processes for green solid phase peptide synthesis.
![Page 21: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/21.jpg)
Figure 2: The swelling properties of PuroSynth™
0
1
2
3
4
5
6
DMF DCM ACN MeTHF GVL
Sw
ellin
g (m
l/g)
PuroSynth Competitor resin 1 Competitor resin 2
Even though the cross-linked polystyrene of PuroSynth™ resin
is insoluble in organic solvents, it swells in aprotic solvents
such as dimethylformamide (DMF), dichloromethane (DCM),
acetonitrile (ACN) and the green solvents
2-methyltetrahydrofuran (MeTHF) and γ-valerolactone (GVL) as
shown in Figure 2.
The advantage of having a 1% DVB cross-linked resin is that
it results in a significant increase in swelling, compared to a
higher cross-linked resin as a polystyrene with 2% DVB.
Resin swelling is a key factor in solid phase peptide synthesis,
since reaction kinetics are diffusion controlled. Consequently,
a resin that swells more will have a higher diffusion rate of
reagents into the core of the matrix, resulting in shorter
reaction times and more complete chemical conversions.
The good solvent properties of PuroSynth™ resins in green
solvents, such as MeTHF and biomass derived GVL open up the
possibility to use these resins on green chemistry processes
for green solid phase peptide synthesis.
Figure 2. The swelling properties of PuroSynth™
09Swelling Properties of PuroSynth™ Resins
Swelling Properties of PuroSynthTM Resins
17
![Page 22: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/22.jpg)
0606
One of the key features of PuroSynth™ products is narrow particle size distribution. ‘Jetting’ - the Purolite® patented manufacturing process, allows the production of uniform particle size, with low uniformity coefficient and high productivity in a continuous mode. This enables a more economic andenvironmentally friendly (reduced waste) process, than classic batch emulsion polymerisation.
The narrow particle size distribution obtained by Jetting is unique to PuroSynthTM resins for SPPS.In SPPS, the uniformity of particle size offers great advantages in terms of uniform kinetics and swelling during peptide synthesis, and so ensures better purity of the final peptide.
Jetting technology allows for the production of large batches (500+ kg) in a very short time
Particle Size Distribution
![Page 23: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/23.jpg)
PuroSynth™ products are manufactured usingmembrane or‘can’ jetting (Figure 3), which uses a very small membrane, through which the polymer is passed. This device is capable of producing uniform particle size resins from ~15 to 250 micron.
PuroSynth™ products have unique performance characteristics. These include a narrow particle size distribution (UC <1.3), a high yield and economical process.
The Jetting Process
![Page 24: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/24.jpg)
Patented, Innovative Manufacturing
Monomer Phase
Aqueous Phase
Pump
Pump
Shaker
oscillations
Organic Phase in membrane can
Continuous Phase
Droplets
Drops formed
Jet
Monomer Feed
Membrane
vibration
Collection Vessel
Polymerization
Heat Exchanger / Condenser
Figure 3: Jetting technology through a membrane for the manufacture of Purosynth™ products
![Page 25: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/25.jpg)
The result of this jetting process is a narrow gaussiandistribution of uniform spherical beads. Figure 4 shows an example of a particle size distribution of PuroSynth™ products. Figure 5 shows an electron microscopy picture for PuroSynth™ products.
Figure 4: Particle size distribution for a batch of PuroSynth™ CTC/S with UC 1.2
![Page 26: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/26.jpg)
Figure 5: Electron microscopy images for Purosynth™ Wang/S
![Page 27: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/27.jpg)
Standard SPPS conditions used are Fmoc/butyl chemistry and DIC-OxymaPure for coupling; 20% piperidine/DMF for Fmoc removal in DMF as a solvent.
Results show that PuroSynth™ resins are efficient in the synthesis of peptides ranging from 5-mer (Figure 6) up to 28-mer (Figure 10). In all cases PuroSynth™ functionalized resins provide excellent purity (measured by HPLC) and recovery of final peptide (determined by weight increase) also, when compared to commercially available products.
Applications
PuroSynth™ resins have been tested by Prof. Fernando Albericio in the University of KwaZulu-Natal, School of Chemistry, Durban (South Africa) and in the University of Barcelona, Department of Organic Chemistry, Barcelona (Spain).
Performances have been evaluated in terms of the purity of the final product and recovery. Synthesis of model peptides has been carried out in both automatic assisted heating microwave systems and in manual mode.
![Page 28: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/28.jpg)
Synthesis of a Pentapeptide (5 amino acids)
12 PuroSynth™ - Applications
PuroSynth™ resins have been tested by Prof. Fernando
Albericio in the University of KwaZulu-Natal, School of
Chemistry, Durban (South Africa) and in the University of
Barcelona, Department of Organic Chemistry, Barcelona
(Spain).
Performances have been evaluated in terms of the purity of the
final product and recovery. Synthesis of model peptides has
been carried out in both automatic assisted heating microwave
systems and in manual mode.
Standard SPPS conditions used are Fmoc/tButyl chemistry
and DIC-OxymaPure for coupling; 20% piperidine/DMF for
Fmoc removal in DMF as a solvent.
Results show that PuroSynth™ resins are efficient in the
synthesis of peptides ranging from 5-mer (Figure 6) up to
28-mer (Figure 10). In all cases PuroSynth™ functionalized
resins provide excellent purity (measured by HPLC) and
recovery of final peptide (determined by weight increase) also,
when compared to commercially available products.
Synthesis of an octapeptide (8 amino acids)
Synthesis of a pentapeptide (5 amino acids)
NH2
O
HO
NH
H
O
HN
H
O
NH O
HN
OH
O
Chemical Formula: C28H37N5O7Exact Mass: 555,27
Molecular Weight: 555,62
H-Tyr-Gly-Gly-Phe-Leu-OH
H-Gly-Pro-Glu-Thr-Ala-Phe-Leu-Lys-OH
NH2O
N
O
HN
O
OHO
NH O
OHHN
O
NH O
HN
O
NH O
OH
Chemical Formula: C40H63N9O12Exact Mass: 861,46
Molecular Weight: 862,00
NH2
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
Figure 6. Synthesis of 5-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM CTC/S
The above acid octapeptide was synthetised with similar purity and
recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S
resins. The amide version of this octapeptide was successfully
synthetised using the PuroSynth™ AM/S containing the Rink linker resin.
0.80
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
AU
0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00
Minutes
PuroSynthTM CTC/S
Figure 7. Synthesis of 8-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM - Applications
Figure 6: Synthesis of 5-mer peptide on PuroSynth™ CTC/S
12 PuroSynth™ - Applications
PuroSynth™ resins have been tested by Prof. Fernando
Albericio in the University of KwaZulu-Natal, School of
Chemistry, Durban (South Africa) and in the University of
Barcelona, Department of Organic Chemistry, Barcelona
(Spain).
Performances have been evaluated in terms of the purity of the
final product and recovery. Synthesis of model peptides has
been carried out in both automatic assisted heating microwave
systems and in manual mode.
Standard SPPS conditions used are Fmoc/tButyl chemistry
and DIC-OxymaPure for coupling; 20% piperidine/DMF for
Fmoc removal in DMF as a solvent.
Results show that PuroSynth™ resins are efficient in the
synthesis of peptides ranging from 5-mer (Figure 6) up to
28-mer (Figure 10). In all cases PuroSynth™ functionalized
resins provide excellent purity (measured by HPLC) and
recovery of final peptide (determined by weight increase) also,
when compared to commercially available products.
Synthesis of an octapeptide (8 amino acids)
Synthesis of a pentapeptide (5 amino acids)
NH2
O
HO
NH
H
O
HN
H
O
NH O
HN
OH
O
Chemical Formula: C28H37N5O7Exact Mass: 555,27
Molecular Weight: 555,62
H-Tyr-Gly-Gly-Phe-Leu-OH
H-Gly-Pro-Glu-Thr-Ala-Phe-Leu-Lys-OH
NH2O
N
O
HN
O
OHO
NH O
OHHN
O
NH O
HN
O
NH O
OH
Chemical Formula: C40H63N9O12Exact Mass: 861,46
Molecular Weight: 862,00
NH2
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
Figure 6. Synthesis of 5-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM CTC/S
The above acid octapeptide was synthetised with similar purity and
recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S
resins. The amide version of this octapeptide was successfully
synthetised using the PuroSynth™ AM/S containing the Rink linker resin.
0.80
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
AU
0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00
Minutes
PuroSynthTM CTC/S
Figure 7. Synthesis of 8-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM - Applications
![Page 29: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/29.jpg)
Synthesis of a Octapeptide (8 amino acids)
12 PuroSynth™ - Applications
PuroSynth™ resins have been tested by Prof. Fernando
Albericio in the University of KwaZulu-Natal, School of
Chemistry, Durban (South Africa) and in the University of
Barcelona, Department of Organic Chemistry, Barcelona
(Spain).
Performances have been evaluated in terms of the purity of the
final product and recovery. Synthesis of model peptides has
been carried out in both automatic assisted heating microwave
systems and in manual mode.
Standard SPPS conditions used are Fmoc/tButyl chemistry
and DIC-OxymaPure for coupling; 20% piperidine/DMF for
Fmoc removal in DMF as a solvent.
Results show that PuroSynth™ resins are efficient in the
synthesis of peptides ranging from 5-mer (Figure 6) up to
28-mer (Figure 10). In all cases PuroSynth™ functionalized
resins provide excellent purity (measured by HPLC) and
recovery of final peptide (determined by weight increase) also,
when compared to commercially available products.
Synthesis of an octapeptide (8 amino acids)
Synthesis of a pentapeptide (5 amino acids)
NH2
O
HO
NH
H
O
HN
H
O
NH O
HN
OH
O
Chemical Formula: C28H37N5O7Exact Mass: 555,27
Molecular Weight: 555,62
H-Tyr-Gly-Gly-Phe-Leu-OH
H-Gly-Pro-Glu-Thr-Ala-Phe-Leu-Lys-OH
NH2O
N
O
HN
O
OHO
NH O
OHHN
O
NH O
HN
O
NH O
OH
Chemical Formula: C40H63N9O12Exact Mass: 861,46
Molecular Weight: 862,00
NH2
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
Figure 6. Synthesis of 5-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM CTC/S
The above acid octapeptide was synthetised with similar purity and
recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S
resins. The amide version of this octapeptide was successfully
synthetised using the PuroSynth™ AM/S containing the Rink linker resin.
0.80
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
AU
0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00
Minutes
PuroSynthTM CTC/S
Figure 7. Synthesis of 8-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM - Applications
Figure 7. Synthesis of 8-mer peptide on PuroSynth™ CTC/S.
12 PuroSynth™ - Applications
PuroSynth™ resins have been tested by Prof. Fernando
Albericio in the University of KwaZulu-Natal, School of
Chemistry, Durban (South Africa) and in the University of
Barcelona, Department of Organic Chemistry, Barcelona
(Spain).
Performances have been evaluated in terms of the purity of the
final product and recovery. Synthesis of model peptides has
been carried out in both automatic assisted heating microwave
systems and in manual mode.
Standard SPPS conditions used are Fmoc/tButyl chemistry
and DIC-OxymaPure for coupling; 20% piperidine/DMF for
Fmoc removal in DMF as a solvent.
Results show that PuroSynth™ resins are efficient in the
synthesis of peptides ranging from 5-mer (Figure 6) up to
28-mer (Figure 10). In all cases PuroSynth™ functionalized
resins provide excellent purity (measured by HPLC) and
recovery of final peptide (determined by weight increase) also,
when compared to commercially available products.
Synthesis of an octapeptide (8 amino acids)
Synthesis of a pentapeptide (5 amino acids)
NH2
O
HO
NH
H
O
HN
H
O
NH O
HN
OH
O
Chemical Formula: C28H37N5O7Exact Mass: 555,27
Molecular Weight: 555,62
H-Tyr-Gly-Gly-Phe-Leu-OH
H-Gly-Pro-Glu-Thr-Ala-Phe-Leu-Lys-OH
NH2O
N
O
HN
O
OHO
NH O
OHHN
O
NH O
HN
O
NH O
OH
Chemical Formula: C40H63N9O12Exact Mass: 861,46
Molecular Weight: 862,00
NH2
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
Figure 6. Synthesis of 5-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM CTC/S
The above acid octapeptide was synthetised with similar purity and
recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S
resins. The amide version of this octapeptide was successfully
synthetised using the PuroSynth™ AM/S containing the Rink linker resin.
0.80
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
AU
0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00
Minutes
PuroSynthTM CTC/S
Figure 7. Synthesis of 8-mer peptide on PuroSynth™ CTC/S.
PuroSynthTM - Applications
![Page 30: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/30.jpg)
The previous acid octapeptide was synthetised with similar purity and recovery using the PuroSynth™ CTC/S and the PuroSynth™ Wang/S resins.
The amide version of this octapeptide wassuccessfully synthetised using the PuroSynth™ AM/S containing the Rink linker resin.
![Page 31: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/31.jpg)
Synthesis of ACP(65-74) peptide (10 amino acids)
Synthesis of ACP(65-74) peptide (10 amino acids)
H2N CHCCH
O
CH3CH3
HN CHC
CH2
O
CH2CNH2
O
HN CHC
CH3
O HN CHC
CH3
O HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
COH
O
HN CHC
CH2
O
OH
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
CNH2
O
HN CHC
HOH
O
Chemical Formula: C47H74N12O16Exact Mass: 1062,53
Molecular Weight: 1063,16
ACP(65-74) is the current model peptide
used to set up synthetic strategies.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
700
800
min0 2 4 6 8 10 12 14
Norm.
-50
0
50
100
150
200
H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH
PuroSynthTM AM/Sfunctionalized with Rink linker
Competitor Resin functionalized with Rink linker
Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.
Synthesis of Jung-Redemann decapeptide (10 amino acids)
H2N CHCCH2
O
HN
HN CHC
CH2
O HN CHC
CH
O
OHCH3
HN CHC
CH
O
OHCH3
HN CHC
CH2
O
CHCH3CH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
OH
HN CHC
CH
O
OHCH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
OHO
CH2SCH3
Chemical Formula: C58H89N11O15SExact Mass: 1211,63
Molecular Weight: 1212,46
Jung-Redemann decapeptide (JR) is the
C-terminal part of a 26-mer peptide,
considered a difficult sequence, more
difficult to manufacture than ACP.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH
PuroSynthTM AM/S Competitor AM Resin
Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin
13PuroSynth™ - Applications
ACP(65-74) is the current model peptide used to set up synthetic strategies.
![Page 32: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/32.jpg)
Synthesis of ACP(65-74) peptide (10 amino acids)
H2N CHCCH
O
CH3CH3
HN CHC
CH2
O
CH2CNH2
O
HN CHC
CH3
O HN CHC
CH3
O HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
COH
O
HN CHC
CH2
O
OH
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
CNH2
O
HN CHC
HOH
O
Chemical Formula: C47H74N12O16Exact Mass: 1062,53
Molecular Weight: 1063,16
ACP(65-74) is the current model peptide
used to set up synthetic strategies.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
700
800
min0 2 4 6 8 10 12 14
Norm.
-50
0
50
100
150
200
H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH
PuroSynthTM AM/Sfunctionalized with Rink linker
Competitor Resin functionalized with Rink linker
Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.
Synthesis of Jung-Redemann decapeptide (10 amino acids)
H2N CHCCH2
O
HN
HN CHC
CH2
O HN CHC
CH
O
OHCH3
HN CHC
CH
O
OHCH3
HN CHC
CH2
O
CHCH3CH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
OH
HN CHC
CH
O
OHCH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
OHO
CH2SCH3
Chemical Formula: C58H89N11O15SExact Mass: 1211,63
Molecular Weight: 1212,46
Jung-Redemann decapeptide (JR) is the
C-terminal part of a 26-mer peptide,
considered a difficult sequence, more
difficult to manufacture than ACP.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH
PuroSynthTM AM/S Competitor AM Resin
Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin
13PuroSynth™ - Applications
Synthesis of ACP(65-74) peptide (10 amino acids)
H2N CHCCH
O
CH3CH3
HN CHC
CH2
O
CH2CNH2
O
HN CHC
CH3
O HN CHC
CH3
O HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
COH
O
HN CHC
CH2
O
OH
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
CNH2
O
HN CHC
HOH
O
Chemical Formula: C47H74N12O16Exact Mass: 1062,53
Molecular Weight: 1063,16
ACP(65-74) is the current model peptide
used to set up synthetic strategies.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
700
800
min0 2 4 6 8 10 12 14
Norm.
-50
0
50
100
150
200
H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH
PuroSynthTM AM/Sfunctionalized with Rink linker
Competitor Resin functionalized with Rink linker
Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.
Synthesis of Jung-Redemann decapeptide (10 amino acids)
H2N CHCCH2
O
HN
HN CHC
CH2
O HN CHC
CH
O
OHCH3
HN CHC
CH
O
OHCH3
HN CHC
CH2
O
CHCH3CH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
OH
HN CHC
CH
O
OHCH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
OHO
CH2SCH3
Chemical Formula: C58H89N11O15SExact Mass: 1211,63
Molecular Weight: 1212,46
Jung-Redemann decapeptide (JR) is the
C-terminal part of a 26-mer peptide,
considered a difficult sequence, more
difficult to manufacture than ACP.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH
PuroSynthTM AM/S Competitor AM Resin
Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin
13PuroSynth™ - Applications
Figure 8: The synthesis of 10-mer peptide on Purosynth™ AM/S & Comparison with compettor AM resin
![Page 33: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/33.jpg)
Synthesis of Jung-Redemann decapeptide (10 amino acids)
Synthesis of ACP(65-74) peptide (10 amino acids)
H2N CHCCH
O
CH3CH3
HN CHC
CH2
O
CH2CNH2
O
HN CHC
CH3
O HN CHC
CH3
O HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
COH
O
HN CHC
CH2
O
OH
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
CNH2
O
HN CHC
HOH
O
Chemical Formula: C47H74N12O16Exact Mass: 1062,53
Molecular Weight: 1063,16
ACP(65-74) is the current model peptide
used to set up synthetic strategies.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
700
800
min0 2 4 6 8 10 12 14
Norm.
-50
0
50
100
150
200
H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH
PuroSynthTM AM/Sfunctionalized with Rink linker
Competitor Resin functionalized with Rink linker
Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.
Synthesis of Jung-Redemann decapeptide (10 amino acids)
H2N CHCCH2
O
HN
HN CHC
CH2
O HN CHC
CH
O
OHCH3
HN CHC
CH
O
OHCH3
HN CHC
CH2
O
CHCH3CH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
OH
HN CHC
CH
O
OHCH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
OHO
CH2SCH3
Chemical Formula: C58H89N11O15SExact Mass: 1211,63
Molecular Weight: 1212,46
Jung-Redemann decapeptide (JR) is the
C-terminal part of a 26-mer peptide,
considered a difficult sequence, more
difficult to manufacture than ACP.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH
PuroSynthTM AM/S Competitor AM Resin
Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin
13PuroSynth™ - Applications
Jung-Redemann decapeptide (JR) is theC-terminal part of a 26-mer peptide, considered a difficult sequence, more difficult to manufacture than ACP.
![Page 34: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/34.jpg)
Synthesis of ACP(65-74) peptide (10 amino acids)
H2N CHCCH
O
CH3CH3
HN CHC
CH2
O
CH2CNH2
O
HN CHC
CH3
O HN CHC
CH3
O HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
COH
O
HN CHC
CH2
O
OH
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
CNH2
O
HN CHC
HOH
O
Chemical Formula: C47H74N12O16Exact Mass: 1062,53
Molecular Weight: 1063,16
ACP(65-74) is the current model peptide
used to set up synthetic strategies.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
700
800
min0 2 4 6 8 10 12 14
Norm.
-50
0
50
100
150
200
H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH
PuroSynthTM AM/Sfunctionalized with Rink linker
Competitor Resin functionalized with Rink linker
Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.
Synthesis of Jung-Redemann decapeptide (10 amino acids)
H2N CHCCH2
O
HN
HN CHC
CH2
O HN CHC
CH
O
OHCH3
HN CHC
CH
O
OHCH3
HN CHC
CH2
O
CHCH3CH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
OH
HN CHC
CH
O
OHCH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
OHO
CH2SCH3
Chemical Formula: C58H89N11O15SExact Mass: 1211,63
Molecular Weight: 1212,46
Jung-Redemann decapeptide (JR) is the
C-terminal part of a 26-mer peptide,
considered a difficult sequence, more
difficult to manufacture than ACP.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH
PuroSynthTM AM/S Competitor AM Resin
Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin
13PuroSynth™ - Applications
Synthesis of ACP(65-74) peptide (10 amino acids)
H2N CHCCH
O
CH3CH3
HN CHC
CH2
O
CH2CNH2
O
HN CHC
CH3
O HN CHC
CH3
O HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
COH
O
HN CHC
CH2
O
OH
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
CNH2
O
HN CHC
HOH
O
Chemical Formula: C47H74N12O16Exact Mass: 1062,53
Molecular Weight: 1063,16
ACP(65-74) is the current model peptide
used to set up synthetic strategies.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
700
800
min0 2 4 6 8 10 12 14
Norm.
-50
0
50
100
150
200
H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-OH
PuroSynthTM AM/Sfunctionalized with Rink linker
Competitor Resin functionalized with Rink linker
Figure 8a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 8b. Comparison with competitor AM resin.
Synthesis of Jung-Redemann decapeptide (10 amino acids)
H2N CHCCH2
O
HN
HN CHC
CH2
O HN CHC
CH
O
OHCH3
HN CHC
CH
O
OHCH3
HN CHC
CH2
O
CHCH3CH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
O
OH
HN CHC
CH
O
OHCH3
HN CHC
CH
O
CH3CH2CH3
HN CHC
CH2
OHO
CH2SCH3
Chemical Formula: C58H89N11O15SExact Mass: 1211,63
Molecular Weight: 1212,46
Jung-Redemann decapeptide (JR) is the
C-terminal part of a 26-mer peptide,
considered a difficult sequence, more
difficult to manufacture than ACP.
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
min0 2 4 6 8 10 12 14
Norm.
0
100
200
300
400
500
600
H-Trp-Phe-Thr-Thr-Leu-Ile-Ser-Thr-Ile-Met-OH
PuroSynthTM AM/S Competitor AM Resin
Figure 9a. Synthesis of 10-mer peptide on PuroSynth™ AM/S Figure 9b. Comparison with competitor AM resin
13PuroSynth™ - Applications
Figure 8: The synthesis of 10-mer peptide on Purosynth™ AM/S & Comparison with compettor AM resin
![Page 35: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/35.jpg)
Synthesis of Thymosin peptide (28 amino acids)
0.80
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
AU
0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00
Minutes
14 PuroSynth™ - Applications and Regulatory
Synthesis of Thymosin peptide (28 amino acids)
N
OO N
O
OON
ON
ON
ON
O
OON
O
ON
O
ON
O
ON
O
O O
NO
NO
ON
O
ON
O
N
NO
OON
ON
O
N
NO
O O
NO
N
NO
N
NO
OO
NO
NO
NO
O O
NO
OO
NO
NO
OO
N NO
NO
Chemical Formula: C127H214N34O53Exact Mass: 3063,51
Molecular Weight: 3065,25
Thymosin is a commercial product on the market.
H-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-NH2
PuroSynthTM AM/S
Figure 10. Synthesis of 28-mer peptide on PuroSynth™ AM/S.
PuroSynthTM - Regulatory
All PuroSynth™ products are made in Europe under an ISO 9001:2008 and ISO 18001:2007 certified facility.
0.80
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
AU
0.00 11.0010.009.008.006.005.004.00 7.003.002.00 12.001.00
Minutes
14 PuroSynth™ - Applications and Regulatory
Synthesis of Thymosin peptide (28 amino acids)
N
OO N
O
OON
ON
ON
ON
O
OON
O
ON
O
ON
O
ON
O
O O
NO
NO
ON
O
ON
O
N
NO
OON
ON
O
N
NO
O O
NO
N
NO
N
NO
OO
NO
NO
NO
O O
NO
OO
NO
NO
OO
N NO
NO
Chemical Formula: C127H214N34O53Exact Mass: 3063,51
Molecular Weight: 3065,25
Thymosin is a commercial product on the market.
H-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-NH2
PuroSynthTM AM/S
Figure 10. Synthesis of 28-mer peptide on PuroSynth™ AM/S.
PuroSynthTM - Regulatory
All PuroSynth™ products are made in Europe under an ISO 9001:2008 and ISO 18001:2007 certified facility.
Figure 10: Synthesis of 28-mer peptide on Purosynth™ AM/S
![Page 36: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/36.jpg)
PuroSynth™ Regulatory
All PuroSynth™ products are made in Europe under an ISO 9001: 2015 and ISO 18001:2007 certified facility.
![Page 37: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/37.jpg)
References
O. Al Musaimi, Y. E. Jad, A. Kumar, J. M. Collins, A. Basso, B. G. de la Torre, F. Albericio, Investigating green ethers for the precipitation of peptides after global deprotection in solid-phase peptide synthesis, Curr. Opin. Green Sustain. Chem. 11 (2018) 99-103, 10.1016/j.cogsc.2018.06.017.
O. Al Musaimi, Y. E. Jad, A. Kumar, A. El-Faham, J. M. Collins, A. Basso, B. G. d. l. Torre, F. Albericio, Greening the Solid-Phase Peptide Synthesis Process. 2-MeTHF for the Incorporation of the First Amino Acid and Precipitation of Peptides after Global Deprotection, Org. Process Res. Dev. (2018), 10.1021/acs.oprd.8b00335.
O. Al Musaimi, S. Serban, Y. E. Jad, Z. Ma, A. Kumar, C. Ji, B. G. d. l. Torre, Nathan R. East, A. Basso, F. Albericio, New Manufacturing Process to produce Highly Uniform Resins with Excellent Performances in solid phase peptide synthesis, Chimica Oggi/Chemistry Today 37(2), 2019, 8-10.
O. Al Musaimi, S. Serban, Y. E. Jad, Z. Ma, A. Kumar, C. Ji, B. G. d. l. Torre, A. Basso, F. Albericio, Jetting manu-facturing of resins for solid-phase peptide synthesis, Chimica Oggi/Chemistry Today 37(1), 2019, 20-23, https://www.teknoscienze.com/tks_article/jetting-manufacturing-of-resins-for-solid-phase-peptide-synthesis/
Al Musaimi, O.; El-Faham, A.; Almarhoon, Z.; Basso, A.; de la Torre, B.G.; Albericio, F. Bypassing Osmotic Shock Dilemma in a Polystyrene Resin Using the Green Solvent Cyclopentyl methyl Ether (CPME): A Morphological Perspective. Polymers 2019, 11, 874.
Al Musaimi, O.; El-Faham, A.; Basso, A.; de la Torre, B.G.; Albericio, F. γ-valerolactone (gvl): An eco-friendly an-choring solvent for solid-phase peptide synthesis. Tetrahedron Letters 2019. 10.1016/j.tetlet.2019.151058
![Page 38: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/38.jpg)
Placing your order
![Page 39: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/39.jpg)
Purosynth™ Wang/S Ordering Information
BULK RESIN LOADING (mmol/g)
Purosynth™ Wang/S
Purosynth™ Wang/S
Purosynth™ Wang/F
Purosynth™ Wang/F
25g
100g
1kg
5kg
20kg
-163
-164
-144
-343
-700
LSPS02401
LSPS02421
LSPS02400
LSPS02420
0.4 - 0.8
0.8 - 1.2
0.4 - 0.8
0.8 - 1.4
PACK SIZE CODE SUFFIX
ORDER NUMBER
![Page 40: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/40.jpg)
Purosynth™ CTC/S Ordering Information
BULK RESIN LOADING (mmol/g)
Purosynth™ CTC/S
Purosynth™ CTC/S
Purosynth™ CTC/F
Purosynth™ CTC/F
25g
100g
1kg
5kg
20kg
-163
-164
-144
-343
-700
LSPS03401
LSPS03421
LSPS03400
LSPS03420
0.4 - 0.8
0.8 - 1.2
0.4 - 0.8
0.8 - 1.2
PACK SIZE CODE SUFFIX
ORDER NUMBER
![Page 41: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/41.jpg)
Purosynth™ AM/S Ordering Information
BULK RESIN LOADING (mmol/g)
Purosynth™ AM/S
Purosynth™ AM/S
Purosynth™ AM/F
Purosynth™ AM/F
25g
100g
1kg
5kg
20kg
-163
-164
-144
-343
-700
LSPS04401
LSPS04421
LSPS04400
LSPS04420
0.4 - 0.8
0.8 - 1.2
0.4 - 1.8
0.8 - 1.2
PACK SIZE CODE SUFFIX
ORDER NUMBER
![Page 42: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/42.jpg)
Purosynth™ MHBA/S Ordering Information
BULK RESIN LOADING (mmol/g)
Purosynth™ MHBA/S
Purosynth™ MHBA/S
Purosynth™ MHBA/F
Purosynth™ MHBA/F
25g
100g
1kg
5kg
20kg
-163
-164
-144
-343
-700
LSPS05401
LSPS05421
LSPS05400
LSPS05420
0.4 - 0.8
0.8 - 1.2
0.4 - 0.8
0.8 - 1.2
PACK SIZE CODE SUFFIX
ORDER NUMBER
![Page 43: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/43.jpg)
Purosynth™ CM/S Ordering Information
BULK RESIN LOADING (mmol/g)
Purosynth™ CM/S
Purosynth™ CM/S
Purosynth™ CM/S
Purosynth™ CM/F
Purosynth™ CM/F
Purosynth™ CM/F
25g
100g
1kg
5kg
20kg
-163
-164
-144
-343
-700
LSPS01401
LSPS01421
LSPS01441
LSPS01400
LSPS01420
LSPS01440
0.4 - 0.8
0.8 - 1.2
1.2 - 1.6
0.4 - 0.8
0.8 - 1.2
1.2 - 1.6
PACK SIZE CODE SUFFIX
ORDER NUMBER
![Page 44: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/44.jpg)
Purosynth™ Rink Amide AM/S Ordering Information
BULK RESIN LOADING (mmol/g)
Purosynth™ Rink Amide AM/S
Purosynth™ Rink Amide AM/S
Purosynth™ Rink Amide AM/F
Purosynth™ Rink Amide AM/F
25g
100g
1kg
5kg
20kg
-163
-164
-144
-343
-700
LSPS06401
LSPS06421
LSPS06400
LSPS06420
0.4 - 0.8
0.8 - 1.2
0.4 - 0.8
0.8 - 1.2
PACK SIZE CODE SUFFIX
ORDER NUMBER
![Page 45: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/45.jpg)
Contact information
Americas150 Monument RoadBala Cynwyd, PA 19004
T +1 800.343.1500T +1 610.668.9090F -+1 484.384.2751
EuropeLlantrisant Business ParkLlantrisantWales, UKCF72 8LF
T +44 1443 229334F +44 1443 227073
Asia PacificRoom 707, C Section Huanglong Century PlazaNo.3 Hangda RoadHangzhou, Zhejiang, China 310007
T +86 571 876 31382F +86 571 876 31385
AlgeriaAustraliaBahrainBrazilCanadaChinaCzech RepublicFranceGermanyIndia
IndonesiaIsraelItalyJapanJordanKazakhstanKoreaMalaysiaMexicoMorocco
New ZealandPolandRomaniaRussiaSingaporeSlovak Republic South AfricaSpainTaiwanTunisia
TurkeyUKUkraineUSAUzbekistan
![Page 46: Purosynth™ Resins for Solid Phase Peptide Synthesis6c02cac8-fd4a-41e6... · solid phase peptide chemistry, due to its chemical stability, compatibility with the majority of organic](https://reader030.vdocuments.mx/reader030/viewer/2022041000/5ea0bda9d26ddd75e43557ae/html5/thumbnails/46.jpg)