298 Persoonia–Volume32,2014

Pseudoneurospora canariensis

299Fungal Planet description sheets

© 2014 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures

Etymology.Canariensis = from Canary Islands, Spain; referring to the geographicaloriginofthefungus.

Mycelium composed of subhyaline to brown, branched, septate, smooth-walled,thin-tothick-walledhyphae,1.5–9µmwide.Ascomata non-ostiolate, superficial to immersed, scatteredtogrouped,atfirstyellowishbrownandtranslucent,becom-ing dark yellowish brown to blackatmaturity, 120–220µmdiam,coveredbymoniliformhyphae,upto17µmwide;setaescarce, sinuose, smooth- and thick-walled, septate, brown, up to150µmlong,3–8µmatthebase,apexrounded;peridium3–4-layered, 5–15µm thick, brown, of textura angularis to globulosa, composed of brown, thick-walled, flattened cells, measuring13–35×12–23µm;ascomatalinitialsarisingassidebranches from mycelium, involving neighbouring hyphae, coiled and contorted.Paraphyses absent.Asci 8-spored, clavate,short-stipitate,200–300×20–25µm,withoutapicalstructures,soonevanescent.Ascospores uniseriate, aseptate, ellipsoidal, (30–)33–36×(18–)22–25µm,atfirstwithahyaline,smooth-walled exosporium and a pigmented, echinulate endosporium, becomingdarkbrown toblackatmaturity,opaque,stronglyverrucose to tuberculate, with a slightly protruding apical germ pore,1.5–2.5µmdiam. Culture characteristics — Colonies on potato carrot agar at-tainingadiameterofmorethan80mmin14dat25°C,velvetytopowdery,brown(5E5–5F5)(Kornerup&Wanscher1984);reversedarkbrown(8F4),blackatthemargins.Coloniesonoatmealagarattaining52–61mmdiamin14dat25°C,velvetytogranular,brown(5E5–5F5);reverseofthesamecolourthanthesurface.Thefungusdoesnotgrowbelow5°Cnorabove40°C.Optimalascomatalproductionoccurredbetween15°Cand25°C.

Typus.spaiN,GranCanaria,PicodeOsorio, from forestsoil,10Aug.2009,coll.M. Calduch & A.M. Stchigel,isol.Y. Marín(holotypeCBSH-21353,culturesex-typeCBS135818=FMR12156;otherstrainCBS136362=FMR12323;LSUsequencesofCBS135818andCBS136362GenBankHG326871andHG326872,respectively;TubsequencesofCBS135818andCBS136362GenBankHG423208andHG423209,respectively;andtef-1sequencesofCBS135818andCBS136362GenBankHG326875andHG326876,respectively;MycoBankMB804881).

Notes—Hitherto,Pseudoneurospora has been a monotypic genus(Garcíaetal.2004).ThemolecularanalysescarriedoutbyNygrenetal.(2011),basedonsevengeneregionsincludingfragmentsoftheβ-tubulin(Tub),translationelongationfactor1-α(tef-1),proteinkinaseC(pkc),28SrDNA,mitogen-activatedprotein kinase-2 (mak-2), nonidentical kinase-1 (nik-1) andhypotheticalprotein-coding(NCU02332)genes,corroboratedPseudoneurospora as a different genus to Neurospora.Ourphylogenetictreeobtainedbysequencingof28SrDNA,Tuband tef-1 loci corroborated our fungus as representing a new species of Pseudoneurospora.Pseudoneurospora canariensis can be distinguished from Pseudoneurospora amorphoporcata, the type species of the genus, by the production of small, non-ostiolateascomata(pyriformandostiolateinP. amorphopor­cata),theabsenceofanapicalringintheasci(presentinthoseof P. amorphoporcata)andbythepresenceofasingleapicalgermporeon theascospores (oneateachend inP. amor­phoporcata).

FungalPlanet278–10June2014

Pseudoneurospora canariensis Y.Marín,Stchigel,DaniaGarcía,Guarro&Cano,sp. nov.

Colour illustrations.PicodeOsorio,GranCanaria,Spain.Ascomata,ascusandascospores.Scalebars(top)=50µm,(bottom)=10µm.

YasminaMarin-Felix,AlbertoM.Stchigel,DaniaGarcia,JosepGuarro&JoséF.Cano-Lira,MycologyUnit,MedicalSchoolandIISPV,UniversityRoviraiVirgili,Reus,Spain;

e-mail: yasmina.marin@urv.cat, albertomiguel.stchigel@urv.cat, dania.garcias@urv.cat, josep.guarro@urv.cat & jose.cano@urv.cat

Neurospora novoguineensis FMR 7269Neurospora retispora FMR 7510Neurospora santi-florii FGSC 8331

Neurospora dictyophora FMR 7511

Neurospora saitoi CBS 435.74T

Neurospora calospora FGSC 958Neurospora terricola CBS 298.63T

Neurospora sitophila FGSC 8770Neurospora tetrasperma FMR 5545

Neurospora crassa FGSC 8900Neurospora dodgei FGSC 1692T

Neurospora cerealis FGSC 959Neurospora hapsidophora CBS 408.82T

Neurospora reticulospora FGSC 6537Pseudoneurospora amorphoporcata CBS 626.80T

Pseudoneurospora canariensis CBS 135818T

Pseudoneurospora canariensis CBS 136362Sordaria fimicola FGSC 2918

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Neurospora novoguineensis FMR 7269Neurospora retispora FMR 7510Neurospora santi-florii FGSC 8331

Neurospora dictyophora FMR 7511

Neurospora saitoi CBS 435.74T

Neurospora calospora FGSC 958Neurospora terricola CBS 298.63T

Neurospora sitophila FGSC 8770Neurospora tetrasperma FMR 5545

Neurospora crassa FGSC 8900Neurospora dodgei FGSC 1692T

Neurospora cerealis FGSC 959Neurospora hapsidophora CBS 408.82T

Neurospora reticulospora FGSC 6537Pseudoneurospora amorphoporcata CBS 626.80T

Pseudoneurospora canariensis CBS 135818T

Pseudoneurospora canariensis CBS 136362Sordaria fimicola FGSC 2918

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98/1

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94/1

100/1

70/0.97

75/1

99/1

100/1

97/1

83/0.97

91/1

0,01

-/1

Maximum likelihood tree obtained from the combined DNA sequencedatafromthreelociofourisolatesandsequencesretrievedfromtheGenBank(Nygrenetal.2011)(TreeBASEID14380).Inthetree,branchlengthsareproportionaltodistance.Bootstrapsupportvalues≥70/Bayesianposteriorprobabilityscores≥0.95are indicatedon thenodes.Sordaria fimicola (FGSC2918)wasusedasoutgroup.Ex-typestrainsofthedif-ferent species are indicated with T.Strainsofthenewspeciesproposed in this study are indicated in bold.