Wojciech Alojzy Świętosławski (1881-1968)

It was begining of long lasting chemistry history of Świętosławski Family.

He was nominated twice for the Nobel Prize for development a static method of cryometric measurement and a new method of testing coal.

T O D A Y

Worldwide recognized formulation and technology development specialists:

Advanced microencapsulation technology leaders.

Technologies being alternatives to current solutions ("fresh air" concepts).

NEXT GENERATION

IN INNOVATIVE CHEMISTRY

House dust allergytreatment

What are the allergens?

A foreign substance that can cause an allergic response in the body but is only

harmful to some people.

Outdoor air polution

Dust Mites’ allergens

Storage mites’ allergens Mold

Pets Cockroach allergens

Indoor asthma triggers

House Dust Mites

A typical mattress may have anywhere from 100,000 to

10 million mites inside.

House Dust Mites

Their favorite food is dander (human skin flakes), also, bedroom carpeting and

household upholstery support high mite populations.

Very small size (250 to 300 microns in length) and translucent bodies, are not visible

to the unaided eye.

An exposure to the mites in the first year of a infants life can trigger a lifelong allergy.

House Dust Mites

20 groups of allergens have been identified.

House Dust MitesMajor allergens: Der p 1, Der f 1Immune response in 75% characterized by IgE antiobodies is related to Der p1 alergen Fecal dropping (20 µm) contains 0,1 ng of Der p 1 (potent protease).

A dose-response relationship between exposure and development of sensitization has an approximate threshold 2 µg/g (10 mites/g floor dust and 100 mites/g of matress dust).

House Dust MitesEpidemiology

About 100 milion people worldwide are sensitive to dust mite allergens

20 ̶ 40 % in Europe.

Number of patients senstive to dust mites double every 10 years.

Samoliński B. i inni: „Epidemiologia nieżytów nosa, astmy i AZS na podstawie badań ECRHS i ISAAC w Polsce”, Alergia, 3, 2007. ECAP.

House Dust MitesEpidemiology

There is sufficient evidence of a causal relationship between dust mite allergen exposure and exacerbations of

asthma individuals specifically sensitized to dust mites. Continual exposure to dust mite allergens is also a

contributing cause of chronic bronchial hyperreactivity.

There is sufficient evidence of a causal relationship between dust mite allergen exposure and the development

of asthma in susceptible children.

Animals

dogs, cats

mices

rats birds

guinea pigs

4 – 10 % of the worldwide population is sensitive to allergens of animals!

About 52 % of patients with that type of hypersensitivity have no domestic animals.

Main allergens are proteins presents in dander, pelt, hair, and saliva, but not in the urine or feces.

DogsMajor allergens:Can f 1 and Can f 2.

Average concentration of Can f 1 allergens in households where dogs are present is 10 000 µg / g of dust.

Allergens are easily aerosolized and widely disseminated throughout the community.

In households without dogs Can f 1 allergen is present at the concentration of 10 µg / g of dust.

Cats (Felis domesticus)

Major allergen Fel d 1 .

95 % of patients allergic to cats make immunoglobulin E (IgE) to Fel d 1.

Minor concentration up to 2 µg / g of Fel d 1 leads to manifestation of allergy.

Average concentration of Fel d 1 allergen in house dust is 3000 µg / g dust.

Rats (Rattus norvegicus) Rat n 1

Mice (Mus musculus) Mus m 1, Mus m 2

Rat n 1 allergen has been measured in the dust of 33 % inner city apartments.

Mice allergens Mus m 1 and Mus m 2 have been measured in 95 % of inner city apartments.

Allergen Mus m 1 causes allergy symptoms in concentration over 1,6 µg / g of dust.18 % of children living in inner city areas are allergic to mice allergens.

Cockroaches

There are multiple allergens from cockroach that have been identified (Bla g I or Per a I, Bla g II, IV, and V) and cloned (Bla g II, IV, and V).

Only insect that has been repeatedly recognized as a common source of indoor allergens.

Comprehensive service of Asthma trigger management

Allergen treatment

Cockroach treatment

Rodent treatment

Fungi

In some cases 20% of the dust content are fungi, the concentration of at the same level all year.

Fungal allergens:

Proteins: Alt a 1, Alt a 2 , Asp f 1HSP: Cla h 1

Enolaze: Cla h 6, Alt a 5, Sac c 1, Can a 1

Why should reduce exposure to allergens?

A strict correlation between exposure to high levels of house dust allergens and allergies.

Need to undertakeactivitiesconsistent with secondaryprevention afterthe first symptomshave appeared in order to inhibit a development of allergy.

High costs of pharmacuticaltreatment and low quality of life in patients.

High incdence of allergies confirmed by epidemiological studies such as asthma, allergic rhinitis and atopic dermatitis.

Allergy preventative approach

There is sufficient evidence of a causal relationship between allergen exposure and exacerbation of asthma.

Allergy preventative approach

The high level of allergen exposure in the environment of this group of infants (4 ̶ 12 months) places them at an increased risk of early sensitization and development of asthma.

Any strategy to reduce asthma prevalence should address these high and avoidable levels.

ARIA RecommendationsAllergic Rhinitis and its Impact on Asthmahttp://www.jacionline.org/article/S0091-6749%2810%2901057-2/fulltext#sec4.1.4

Environmental recommendations

1. In infants and school children it is recommended to apply integrated programme of reduction of exposure to allergens.

2. Special attention should be paid to places children spend most of their time (bedrooms, kindergarten).

Further recommendations

Relatively low value on the burden and cost of using multiple preventive measures e.g.

• encasings to parental and child’s bed;

• washing bedding and soft toys at temperature exceeding 55°C (131°F), use of chemical treatment, smooth flooring without carpets, etc., and relatively high value on an uncertain small reduction of the risk of developing wheeze or asthma;

• for some children at lower risk of developing asthma and in certain circumstances an alternative choice will be equally reasonable.

World Allergy Organizationrecommendations

Create a more integrated and holistic approach to the diagnosis 1.

and management of allergic diseases.

Increase public awareness of allergic diseases and their 2.

prevention.

Provide greater education at the primary healthcare level and 3.

to non-allergy-oriented secondary care specialists.

4. Train medical students and other health care professionals to collaborate with specialists in providing integrated care for allergy patients.

5. Institute environmental control measures by the lowering of indoor and outdoor air pollution, tobacco smoking, and allergen and drug exposures.

6. Encourage a preventative approach to allergic diseases, emphasizing the importance of continued research into disease causation and management.

7. Develop national allergy action plans to promote the prevention of allergic diseases and immune tolerance, aiming to decrease the burden of allergic diseases.

Technology and mode of action

How long protectiondoes Allergoff®PRO

provide

This slide will be played for the next

6 months. As long as Allergoff®PRO protects you from

the house dust allergens!.

Mode of action

Immobilization

Before treatment

Immobilization

Immobilization

After treatment

Modification of the spatialstructure leads to

neutralisation of the allergicpotential of allergens.

Allergen neutralisation

Allergoff®PRO aggregates the allergens, thus facilitatingvacuum cleaning. The aggregated allergens cannot float in the air thus providing a reduction in the risk of their pulmonary aspiration.

Allergen agregation

Application

RECOMMENDED PERCENTAGE AND APPLICATION RATE FOR 100m2

250 ml of concentrate / 1,75 liters of water.

Apply in a rate of ca. 20 ml / 1 m2.

Application sites

Allergen agregation

30-days post-treatment

Where to apply

Beds and other sleeping places –treat quilts and pillows

Mattresses Carpets and carpeted floors

Upholstered furniture Curtains Plush toys

Applicaion tips

Spray the objects from both sides.

Remove cases from

mattresses, quilts and

pillows.

Surfaces that have been

sprayed can be used one hour

after application, when the product

has dried.

Ventilate the room.

Do not vacuum mattresses and

upholstered furniture after

application.

Start to vacuum carpets and

carpeted floors that have been

sprayed on 3 days after application.

Advantages of Allergoff®PRO

Long-term efficacy.

E

Easy removalof the allergens.

R

Reduces the risk of pulmonaryaspiration of allergens.

R

No stain, no odour formula.

F

Immobilization-basedAdvanced pest management

Saving lives…

Active adhesion to vector’s exoskeleton.

Spreading

Water 3D-ISN film

Permeable 3-Dimensional immobilizing (gluing) network structure

Immobilization

Spiracles leads to respiratory system

Mechanical immobilization of spiracles’ valve

Filtering apparatus

Trachea

ProVecta®

Immobilized(blocked) valveof spiracle

Mechanical immobilization of spiracles’ valve

Valve blocked in closed position

Valve blocked in open position

Spiracle Spiracle

Decreased diameter of trachea

Mechanical immobilizationof spiracles’ valve

Immobilization

Immobilization

Test location: PanamaDate: July 2016

Test formulation: ProVecta (Freeze!) Concentration: 1.0%Mosquito species: Culex quinguefasciatus in plastic net cagesULV generator (hand-operated)Application distance: 7 metres

Immobilization-based Advanced Vector control

ImmobilizedCulexmosquitoes

Knock-down after 15 minutes

100% mortality up to 36 hours

Immediate inhibition of feeding

Test location: PhilippinesDate: September 2016

Test formulation: ProVecta (Freeze!) Concentration: 1.0%Mosquito species: Culex quinguefasciatus in nylon mesh bagULV generator (hand-operated)Application distance: 10 metres

Immobilization-based Advanced Vector control

Test location: PhilippinesDate: September 2016

Immobilization-based Advanced Vector control

• Knock-down after 12 minutes

• Immediate inhibition of feeding

• 75% mortality after 3 hrs.

• 100% mortality up to 36 hours

New approach to control of hematophagous arachnids:

Red poultry mite (Dermanyssus

gallinae)

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Chewing lice (Mallophaga)

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Soft ticks(Argas reflexus,

A. polonicus)

Before application

After application

Efficacy ofDergall® against Dermanyssus gallinae

Hens are happy High reduction of ectoparasite populationEffect of the treatment – immobilized parasites

Post-treatment

Side-by-side comparison of NFM counts for each treatment, separated by count date.

Safety?

Studies Date Findings

Acute Toxicty studies 2013

The technology / formulation does not pose

any health risks to mammals.

Environmental Impact Studies

2013No negative impact on

environment.

Polinator Impact Studies 2013No negative findings -

Formulation does pose any risk to Honey Bees

Pathomorphological Studies

2015

Confirmed no pathological changes in the internal

structure of organs of the birds

Studies on Development of Chicken Embyros

2016No Negative effects on

Chicken embryodevelopment (HET-CAM)

Safety assessment of selected disinfectants and pest control agents used in poultry houses

Dergall : 3D-IPNS technology;Siltac: polymeric surfactants;Preparation A: mixture composed of glutaraldehyde, didecyldimethylammonium chloride, quaternaryammonium compounds, benzyl-C12-C16-alkyldimethyl and chloride;Preparation B: containing stabilized peracetic acid with hydrogen peroxide.

Concentrations: 0.0, 0.3%, 0.6% 1%; 3%; 6%; 25%; 50% and 100%

0

5

10

15

100% 50% 25% 6% 3% 1% 0.6% 0.3%

toxic

ity

[ L

uep

ke’s

sc

ale

]

Concentration

Dergall Siltac Preparat A (aldehyd glutarowy) Preparat B (kwas nadoctowy+nadtlenek wodoru)

No

n to

xic

weak

mo

de

rate

stro

ng

DergallSiltac

Dergall

Chick embryos (Ross 308) – 8th day of incubation;n = 10 embryos / preparation / concentration

Safety assessment of Dergall on the embryo development in sprayed eggs

IncubationTemperature 37.8°C, RH 50%

Candling of hatching eggsAfter 7, 14 and 19 days of incubation (E7, E14, E19); unfertilizedor dead eggs were rejected;

WeightingBefore incubation and on the 19th day of incubation (E19);

HatchThe hatched chick were weighted and grade of healing of thenavel was scored from 0 (completely healed) to 5 (inflammationof the navel)

70%

80%

90%

100%

110%

hatc

habi

lity

[%]

no treated concentration 0% concentration 0.3%

concentration 0.6% concentration 3% concentration 6%

a

ababab

bb

The hatchability of eggs sprayed aqueous solutions of Dergall at the concentration of 0, 0.3%, 0.6%, 3%, 6%

ab – values marked various letter differ significantlys

Application of Dergall at the concentration of 3%and lower does not cause any negative reaction toanimals, and humans or decrease the hatchabilityof eggs.

Antimicrobial activitybased on physical mode

of action

Inhibitory effect of 3D-IPNS against „wild” strain of Stapylococcus aureus

Electrostatic interactionwith microbial cell

Inhibitory effect of 3D-IPNS against „wild” strain of Escherichia coli

Electrostatic interactionwith microbial cell

Inhibitory effect of 3D-IPNS against „wild” strain of Pseudomona aeruginosa

Electrostatic interactionwith microbial cell

Inhibitory effect of 3D-IPNS against „wild” strain of Streptococcus pyogenes

Electrostatic interactionwith microbial cell

Noninsecticidal

Nopoison

Noeffects to long term exposure

Nochronicexposure

Nothermal- orphotolability

NoResistancy

Does not promote creation of resistantinsect populations throughcontinous exposure

Safe for

Operators

Harmless to

mammals

Nonegative

environmental impact

Helps to

reduce the risk of vector-transmitteddiseases

Thank You for Your Attention!

Thank you for your attention!