postharvest diseases of avocados- from orchard to market...
TRANSCRIPT
The New Zealand Institute for Plant & Food Research Limited
Postharvest diseases of avocados-
from orchard to market
A reminder
Dr Kerry Everett
Mt Albert Research Centre
Auckland, New Zealand
The New Zealand Institute for Plant & Food Research Limited
Two postharvest diseases
Ø Stem-end rots Ø Body rots
The New Zealand Institute for Plant & Food Research Limited
The New Zealand Institute for Plant & Food Research Limited
The New Zealand Institute for Plant & Food Research Limited
What causes rots?
0
2
4
6
8
10
12
14
16
18
20
C.a. C.g. B.p. B.d. P.
Mea
n n
o.
isola
tions/1
00 f
ruit
Fungus
body rots
stem-end rots
Colletotrichum acutatum
Colletotrichum gloeosporioides
Botryosphaeria parva
Botryosphaeria dothidea
Phomopsis sp.
The New Zealand Institute for Plant & Food Research Limited
Are rots a problem?
• USA
• Three strikes and you are out (late ’90’s)
• Rots
• Measles = rots in green fruit
• Australia
• 50% of consumers do not repeat purchase because of poor quality
due to rots (1990’s)*
• Large backlog of NZ fruit in the market place leading to extended
storage and fruit age problems (1997)
• Three issues
• rots
• fruit age
• measles
*Ledger, S., Campbell, T., Banks, A., Atkinson,I, Kernot, I. and Fullelove, G. 1993
Internal quality of avocados in retail shops.
Queensland Department of Primary Industries Internal Report (Avoman). 23pp.
The New Zealand Institute for Plant & Food Research Limited
What did we do to fix it?
Research
Avocado Rot Survey
• Three year survey of orchards to identify cultural and grower
practices that affected rots
• Followed it up with replicated trials
Determined:
• the storage limits of fruit
• the criteria for infection
• the relationship between lenticel damage and measles
Logistics
• Reviewed the research that had already been conducted in NZ
• Tightened up timeframes in the logistics pathway
• Cooperation amongst exporters to rationalise flow of avocados
to the market
The New Zealand Institute for Plant & Food Research Limited
Avocado Rot Survey
• Sampled 8 orchards in January each year for 3 years
• Sampled an additional 13 orchards for 2 of the 3 years
• Assessed 100 fruit from each orchard for rots
• Collected orchard parameters including spray diaries and weather
• Analysed the data
Everett KR, Boyd LM, Pak HA, Cutting JGM 2007.
Calcium, fungicide sprays and canopy density influence
postharvest rots of avocado. Australasian Plant Pathology
36(1): 22-31.
The New Zealand Institute for Plant & Food Research Limited
Fungicide sprays reduce rots
0 2 4 6 8 10 12
0
5
10
15
20
25
30
35
40
45
50R
2=83%
P=0.002
Y = 44.0 - 2.4 X
Mean
no
. o
f fr
uit
wit
h s
tem
-en
d r
ots
(%
)
Mean no. of fungicide applications
0 2 4 6 8 10 12
0
5
10
15
20
25
30
R2=74%
P=0.04
Y = 16.0 + 3.4 X - 0.4 X2
Mean
no
. o
f fr
uit
wit
h b
od
y r
ots
(%
)
Mean no. of fungicide applications
The New Zealand Institute for Plant & Food Research Limited
Nutrition affects body rots
0.045 0.050 0.055 0.060 0.065 0.070 0.075 0.080 0.085 0.090 0.095 0.100
0
5
10
15
20
25
30
35
40
45
50
0
5
10
15
20
25
30
35
40
45
50
2000
no.
of
fruit w
ith b
ody r
ots
(%
)
Ca+Mg
K
Y= 396.5 exp(-X/0.02) - 0.51
R2 = 56%
2001
The New Zealand Institute for Plant & Food Research Limited
Rainfall inversely related to body rots
0 20 40 60 80 100 120
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
P=0.03
R2=57.4%
Y=0.25-0.002X
Bo
dy
ro
ts (
me
an
se
ve
rity
)
mm rainfall 20 days before picking
The New Zealand Institute for Plant & Food Research Limited
Lo
g10 (n
o. o
f fr
uit
wit
h
bo
dy
ro
ts )
C
a+M
g
K
Rai
nfa
ll (
mm
)
Ca+Mg
K
Rainfall (mm) Temperature (°C)
0.050 0.055 0.060 0.065 0.070 0.075 0.080
0.8
1.0
1.2
1.4
1.6
1.8
P=0.02
R2=70.4%
Y=2.5-21.6X
60 80 100 120 140 160 180 200
0.8
1.0
1.2
1.4
1.6
1.8
P=0.03
R2=63.3%
Y=1.78-0.006X
16.2 16.4 16.6 16.8 17.0 17.2 17.4
0.050
0.055
0.060
0.065
0.070
0.075
0.080
P=0.024
R2=67.2%
Y=0.399-0.03X
60 80 100 120 140 160 180 200
0.050
0.055
0.060
0.065
0.070
0.075
0.080
P=0.03
R2=66.2%
Y=0.04+0.0002X
16.2 16.4 16.6 16.8 17.0 17.2 17.4
0.8
1.0
1.2
1.4
1.6
1.8 P=0.01
R2=75.6%
Y=14.1-0.77X
16.2 16.4 16.6 16.8 17.0 17.2 17.4
60
80
100
120
140
160
180
200
Rainfall inversely related to body rots
30 days BH 40 days BH
The New Zealand Institute for Plant & Food Research Limited
Canopy index influences body rots dead branches + (canopy density x leaf height inside canopy)/tree spacing
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45 P=0.02
R2=23.3%
Y=0.03+0.12X
mean s
everi
ty (
body r
ots
)
canopy index
The New Zealand Institute for Plant & Food Research Limited
3 way analysis of variance (body rots)
Fitted : logten.ca.mg.k. + no..of.months.sprayed + canopy.index
body.r
ots
0.05 0.10 0.15 0.20 0.25 0.30
0.1
0.2
0.3
0.4
R2=67.4%
P=0.000125
The New Zealand Institute for Plant & Food Research Limited
Replicated trials
1. Nutrition, canopy management and irrigation
2. Fungicide spray trial
3. Fruit age
The New Zealand Institute for Plant & Food Research Limited
Nutrition, canopy management and root
health (root health = irrigation + Phytophthora control)
R + R + R - R - R + R + R - R -
x x x x x x x x
xN+ x xN+ x xN+ x xN+ x
xN- x xN- x xN- x xN- x
x x x x x x x x
x xN- x xN- x xN- x xN-
x xN+ x xN+ x xN+ x xN+
x x x x x x x x
Key
symbol explanation
x Tree
Assessed trees
C-
R Root health
C Canopy management
N Nutrition
Two orchards- organic and conventional
C- = no canopy management
•Dead branches removed from the canopy of all other trees,
•the lower branches were trimmed to a height of 1 m.
The New Zealand Institute for Plant & Food Research Limited
The New Zealand Institute for Plant & Food Research Limited
Nutrition, canopy management and
irrigation
R + R + R - R - R + R + R - R -
x x x x x x x x
xN+ x xN+ x xN+ x xN+ x
xN- x xN- x xN- x xN- x
x x x x x x x x
x xN- x xN- x xN- x xN-
x xN+ x xN+ x xN+ x xN+
x x x x x x x x
Key
symbol explanation
x Tree
Assessed trees
C-
R Root health
C Canopy management
N Nutrition
Two orchards- organic (5-6 yo) and conventional (15-20 yo)
C- = no canopy management
•Dead branches removed from the canopy of all other trees,
•the lower branches were trimmed to a height of 1 m.
R+ = Root health
• irrigated
• treated with phosphorous acid or mulch (organic orchard)
N+ = Nutrition
•Calcium was applied to the ground under trees.
The New Zealand Institute for Plant & Food Research Limited
Results
Factors Body rots Stem-end rots Total rots
Tree n.s. n.s. n.s.
Box n.s. n.s. n.s.
N 0.004 n.s. 0.002
R <0.0001 <0.0001 <0.0001
C n.s. n.s. n.s.
Factors Body rots Stem-end rots Total rots
Tree n.s. n.s. n.s.
Box 0.05 n.s. n.s.
N 0.05 n.s. n.s.
R n.s. n.s. n.s.
C n.s. n.s. 0.03
Conventional orchard (trees 15-20 years old)
Organic orchard (trees 5-6 years old)
The New Zealand Institute for Plant & Food Research Limited
Fungicide spray trial
Aims:
• More efficaceous fungicides
• Regional differences?
Procedures
• In vitro screening
• Replicated spray trials
The New Zealand Institute for Plant & Food Research Limited
In vitro testing
Fungicide
C. acutatum
C. gloeosporioides
B. parva
B. dothidea
Phomopsis sp.
boscalid <0.1 <0.1 18.2 27.7 74.8
boscalid/
pyraclostrobin
7.0 0.2 2.3 0.2 0.2
KocideÒ 2000DS 0.4 8.1 0.6 15.6 1.5
KocideÒ 3000 7.3 140.9 0.3 4.8 0.1
ChampÔ DP 1.0 57.6 0.1 11.4 2.7
copper
hydroxosulphate
0.1 2.3 4.0 98.2 1.5
dithianon <0.1 <0.1 3.1 18.5 <0.1
fluazinam 0.1 1.9 0.1 0.1 0.1
EC 50 spore germination
Everett KR, Timudo-Torrevilla OE 2007. In vitro fungicide testing for
control of avocado fruit rots. New Zealand Plant Protection 60: 99-103
http://www.nzpps.org/.
Everett KR, Owen SG, Cutting JGM 2005. Testing efficacy of fungicides
against postharvest pathogens of avocado (Persea americana cv. Hass).
NZ Plant Protection 58: 89-95. http://www.nzpps.org/
The New Zealand Institute for Plant & Food Research Limited
Fungicide
C. acutatum
C. gloeosporioides
B. parva
B. dothidea
Phomopsis sp.
boscalid 1921.0 >* 837.7 2154.4 >
boscalid/
pyraclostrobin
0.2 0.1 0.2 0.0 0.8
KocideÒ 2000DS 2540.7 647.3 304.7 153.2 105.8
KocideÒ 3000 867.2 297.1 228.1 118.1 101.8
ChampÔ DP 346.7 257.2 1321.7 197.0 193.3
copper hydroxosulphate 910.5 402.1 1645.3 749.9 543.0
dithianon 44.2 201.4 989.6 540.3 17.2
fluazinam 7.8 1.7 6.6 4.3 0.5
*EC50 value is greater than the highest concentration used
In vitro testing
EC 50
mycelial growth
Everett KR, Timudo-Torrevilla OE 2007. In vitro fungicide testing for
control of avocado fruit rots. New Zealand Plant Protection 60: 99-103.
http://www.nzpps.org/
Everett KR, Owen SG, Cutting JGM 2005. Testing efficacy of fungicides
against postharvest pathogens of avocado (Persea americana cv. Hass).
NZ Plant Protection 58: 89-95. http://www.nzpps.org/
The New Zealand Institute for Plant & Food Research Limited
Field trials
fluazanim
dithia
non
boscalid
/pyra
clo
str
obin
boscalid
Bio
sta
rt
Cham
p D
P
Kocid
e 3
000
untr
eate
d --
0
10
20
30
40
50
60
70
***
*
Body rots
me
an
seve
rity
(0
-10
0 s
cale
)
Treatment
fluazanim
dithia
non
boscalid
/pyra
clo
str
obin
boscalid
Bio
sta
rt
Cham
p D
P
Kocid
e 3
000
untr
eate
d --
0
2
4
6
8
10
Stem-end rots
me
an
seve
rity
(0
-10
0 s
cale
)
Treatment
Everett, K.R., Timudo-Torrevilla, O.E., Hill, G.N. and Dawson, T.E. 2009.
Field testing alternatives to copper for controlling avocado fruit rots. NZ
Plant Protection 61: 65-69. http://www.nzpps.org/
The New Zealand Institute for Plant & Food Research Limited
ANOVA Factors SER BR
Treatment <0.0001 <0.0001
Replicate n.s. n.s.
Region <0.0001 <0.0001
Orchard <0.0001 <0.0001
Fungicide product1 Active ingredient Chemical group
Rate applied Product/100 L
PristineÒ pyraclostrobin/ boscalid
strobilurin/ carboxamide
60 g
KocideÒ Opti copper hydroxide copper 90 g
ShirlanÒ fluazinam pyridinamine 100 mL
4 orchards per region
4 treatments
100 fruit per treatment
8 applications (March to October)
Harvested November
The New Zealand Institute for Plant & Food Research Limited
Regional Field Trials
Pyraclostrobin/boscalid Fluazinam Copper hydroxide Untreated
0
10
20
30
40
50
60
c b b a
Ro
t In
cid
en
ce
(%
)
Treatment
SER
BR
a b b b
Everett, K.R., Pushparajah, I.P.S., Taylor, J.T., Astill, M.S., King, D.B.
and Ah Chee, A. 2010. Fungicide spray trial in three avocado growing
regions in New Zealand. NZ Avocado Growers' Association Annual
Research Report Vol. 8: 99-110. available on-line at
www.avocadosource.com
The New Zealand Institute for Plant & Food Research Limited
Storage limits of fruit
• Fruit inoculated with Colletotrichum acutatum
• Then placed in coolstore (5.5oC)
• Week 0 fruit was held at 20oC and assessed for SER after 1,3, 4, 6 and 7 days
• After 2 weeks fruit was removed from the coolstore, then every week for 7 weeks
• The fruit was assessed for SER and firmness on 5 occasions as for day 0:
usually 1,3, 4, 6 and 7 days after removal from the coolstore
0 2 3 4 5 6 7 Weeks in coostorage (5.5oC) 5.5oC
20oC
= 20 inoculated fruit &
20 uninoculated fruit
The New Zealand Institute for Plant & Food Research Limited
Storage limits of fruit
0 7 14 21 28 35 42 49
0
10
20
30
40
50
60
70
80
90
100
Ripeness of 5 by hand inoculated
control
ste
m e
nd
ro
t in
cid
en
ce
(%
)
time in coolstorage (days)
0 7 14 21 28 35 42 49
0
10
20
30
40
50
60
70
80
90
100
inoculated
control
ste
m e
nd
ro
t i
nc
ide
nc
e (
%)
time in coolstorage (days)
Fruit assessed when ripe
Everett, K.R. & Pak, H.A. 2002
Patterns of stem-end rot development
in coolstorage.
NZ Avocado Growers’ Association
Annual Research Report 2: 68-74
Dixon, J., Pak, H.A., Mandemaker, A.J.,
Smith, D.B., Elmsly, T.A. & Cutting, J.G.M. 2003
Fruit age management: the key to successful long
distance export of New Zealand avocados.
NZ Avocado Growers’ Association
Annual Research Report 3: 60-65
Fruit assessed when hard
The New Zealand Institute for Plant & Food Research Limited
Infection criteria
10 12 14 16 18 20 22 24 26 28 30
0
10
20
30
40
50
60
70
80
90
100
C. gloeosporioides 24 hours
Y = 6.75 x (T-20)0.958
x (28.25-T)0.441
T optimum=25.6oC
R2=94.3%
P=0.006
% s
po
re g
erm
ina
tio
n
Temperature (oC)
10 12 14 16 18 20 22 24 26 28 30
0
10
20
30
40
50
60
70
80
90
100
C. acutatum 24hr
Y=3.76 x (T-Tmin
)1.04
x (Tmax
-T)0.73
T optimum=23.3oC
R2=86%
P=0.003
% s
po
re g
erm
ina
tio
n
Temperature (oC)
10 12 14 16 18 20 22 24 26 28 30
0
10
20
30
40
50
60
70
80
90
100
B. parva 24hr
% s
po
re g
erm
ina
tio
n
Temperature (oC)
10 12 14 16 18 20 22 24 26 28 30
0
10
20
30
40
50
60
70
80
90
100
Y = 60
1+e-1(x-17)
R2=46.2%
P=0.06
Phomopsis 24hr
% s
po
re g
erm
ina
tio
n
Temperature (oC)
Everett, K.R. & Pak, H.A. 2002
Infection criteria for pathogens
causing body rots in avocados
NZ Avocado Growers’ Association
Annual Research Report 2: 54-58
The New Zealand Institute for Plant & Food Research Limited
Jan
uary
Feb
ruary
Marc
h
Ap
ril
May
Ju
ne
Ju
ly
Au
gu
st
Sep
tem
ber
Octo
ber
No
vem
ber
Decem
ber
Jan
uary
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Phomopsis sp.
C. acutatum
C. gloeosporioides
Kaitaia
Whangarei
TePuke
Tem
pera
ture
(oC
)
10 12 14 16 18 20 22 24 26 28 30
0
10
20
30
40
50
60
70
80
90
100
B. parva 24hr
% s
po
re g
erm
ina
tio
n
Temperature (oC)
Everett & Pak 2002
NZ Avocado Growers’ Association
Annual Research Report 2: 68-74. www.avocadosource.com
The New Zealand Institute for Plant & Food Research Limited
Literature review - Postharvest fungicides
Treatment No. of trials % efficacy
Flusilazole 1 68.8
Permanganate (acidified) 1 58
Prochloraz 10 55.3
Benomyl 7 52.7
Hypochlorite 5 36.6
Benzalkonium chloride 1 18.8
Thiabendazole 2 17.8
10% ethanol 2 13
Phosphorous acid 2 4.8
Carbendazim 2 2.5
Kresoxim-methyl 2 8
Boscalid/pyraclostrobin 1 0
Everett, K.R. 2002 Avocado fruit rots: A review of industry funded
research. NZ Avocado Growers’ Association Annual Research Report 2:
8-16. www.avocadosource.com
The New Zealand Institute for Plant & Food Research Limited
Literature review - Postharvest fungicides
Prochloraz treatment- apply as soon after harvest as possible
Hours after harvest fruit treated
Not treated 0 2 4 12 24
% rots 73 28 27 35 34 40
y = 3.1x + 23.5 R² = 0.8371
0
10
20
30
40
50
0 2 4 12 24
% r
ots
Hours after harvest fruit treated
Everett, K.R. 2002 Avocado fruit rots: A review of industry funded research.
NZ Avocado Growers’ Association Annual Research Report 2: 8-16 www.avocadosource.com
The New Zealand Institute for Plant & Food Research Limited
Finally- to reduce postharvest issues
Apply fungicides
Irrigate the trees and control Phytophthora
Apply calcium
Minimise handling damage
Apply fungicides close to harvest
Get fruit to the marketplace as quickly as possible
Rationalise the flow of fruit to the marketplace so there are no gluts leading
to fruit age problems
The New Zealand Institute for Plant & Food Research Limited
The New Zealand Institute for Plant & Food Research Limited
Thanks to Ian Hallett, Henry Pak, Shamini Pushparajah,
Olimpia Timudo, Jonathan Rees-George
The New Zealand Institute for Plant & Food Research Limited
Treatment No. of trials % efficacy
Methyl jasmonate 1
0
76% ethanol 1 0
Neem oil 1 0
Serenade 1 0
Sporekill 1 0
Antioxidants
(Xedaphen-20 and
BHT + citric acid)
1 0
CO2 shock 1 0