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THE INFI/J-£~CE OF CATECHOLAMINE ON NEUROPEPTIDE Y-LIKE IMMUNOREACTIVE NEURONS IN RAT CI~,EHRAL

CORTEX AND BASAL GANGLIA

MASATO MIYAKE I , HITOSHI OKAMURA 2, TOMOKO SUGANO 3, FUMIO KAWAKAMI I , ~JI FUKUI I AND YASUHIKO

IBATA 2

Department of Psychiatry I , Anatomy 2 and Pediatrics 3, Kyoto Prefectual University of

Medicine, Kamigyo-ku, Kyoto 602.

Neuropeptide Y(NPY) exists in very high concentrations in the central nervous system. This

peptidehasbeen reported to co-exist with noradrenaline or adrenaline in neurons in the brain

stem. For elucidating the interaction of NPY and catecholamine, we investigated the influence

of catecholamine on NPY-like immunoreactive fibers in the rat brain by using peroxidase anti-

peroxidase immunocytochemistry.

Male Wistar rats were used in this study. Chronic haloperidol treatment (25mg/kg/day,

6days) markedly increased in the number of NPY-like immunoreactive fibers and terminals in the

cerebral cortex, especially in the cingulate, insular and piriform cortex. NPY-like immuno-

reactive mossy fiber endings in CA3 intensively increased their immunoreactivity. On the

contrary, immunopositive fibers and terminals decreased in the caudate putamen and the nucleus

accumbens. Treatment with sulpiride (330mg/kg/day, 6days), a dopamine receptor blocker, led to

similar results to that of haloperidol. On the other hand, in the cerebral cortex

methamphetamine (20mg/kg/day, 6days) treatment decreased immunoreactive fibers, and propranorol

(10mg/kg/day, 6days) treatment made no remarkable change. These findings suggest that the NPY-

containing neuronal system in the cerebral cortex and the basal ganglia is under the strong

influence of the dopaminergic system.

ONTOGENY OF [3H]-NEUROTENSIN BINDING SITES IN THE RAT CEREBRAL CORTEX:

AUTORADIOGRAPHIC STUDY

HIROSHI KIYAMA I, SHINOBU INAGAKI 2, SHOZO KITO 2 and MASAYA TOHYAMA 3

IDept.Neuroanatomy,Inst.Higher Nervous Act. , 3Dept.Second Anatomy, Osaka

Univ. Med.Sch.4-3-57 Nakanoshima Kitaku, Osaka(530). 2Dept In ternal Ned.

Hiroshima Univ. Med. Sch.

T h e o n t o g e n y o f t h e n e u r o t e n s i n - b i n d i n g s i t e s i n t h e r a t c a u d a l c o r t e x

s u c h a s t h e r e t r o s p l e n i a l c o r t e x , t h e v i s u a l a r e a c o n t a i n i n g a r e a 1 7 a n d 1 8 ,

t h e c a u d a l s o m a t o s e n s o r y a r e a a n d t h e t e m p o r a l a r e a w a s e x a m i n e d b y

a u t o r a d i o g r a p h y . D u r i n g t h e e a r l y p o s t n a t a l p e r i o d ( f r o m b i r t h t o d a y 1 0 ) , a

v e r y h i g h d e n s i t y [ 3 H ] - n e u r o t e n s i n b i n d i n g s i t e w a s o b s e r v e d i n t h e c a u d a l c o r -

t i c a l a r e a , b u t t h e n u m b e r o f b i n d i n g s i t e s d e c r e a s e d m a r k e d l y w i t h a g e . On t h e

o t h e r h a n d , t h e v e n t r a l t e g m e n t a l a r e a a n d s u b s t a n t i a n i g r a p a r s c o m p a c t a h a d a

l o w d e n s i t y o f b i n d i n g s i t e a t b i r t h , b u t t h e d e n s i t y i n c r e a s e d u n t i l a b o u t d a y

15 a n d r e m a i n e d e v e n i n t h e a d u l t r a t . T h e s e f i n d i n g s w e r e c o n f i r m e d b y t h e

b i o c h e m i c a l b i n d i n g a n a l y s i s .