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NATIONAL BUREAU OF PLANT GENETIC RESOURCES New Delhi – 110 012

Proceedings of the First Institute Research Council (IRC) Meeting of 2012

(14-17May, 2012)

At the outset, Dr. KK Gangopadhyay, Member Secretary, IRC welcomed Dr. JS Sandhu ADG (Seed), Dr. KC Bansal, Director, NBPGR and Chairman, IRC and all members of IRC in the meeting and introduced the newly joined scientists (Annexure-I). Dr Sandhu in his remarks appreciated the efforts of NBPGR in evaluation and characterization of large number of germplasm accessions of wheat and chickpea and suggested to undertake the work keeping in view the available manpower, expertise and resources so as to have the quality data. He informed that about 15% of the budget allocation may be kept for renovation and maintenance of infrastructure. He suggested having the achievable action points from IRC meeting. The Chairman, IRC, in his welcome address, informed the house that NBPGR had been identified for two platforms viz., Agro-biodiversity and Genomics in XII Plan and appreciated the efforts of the Bureau’s scientists in undertaking the evaluation of wheat germplasm (~22,000 accessions) at three locations (Wellington, Issapur and Hisar) and chickpea (~19,000 acc.) at Rahuri. He also informed about the MoU signed between DRDO and ICAR regarding conservation of germplasm under permafrost conditions. He emphasized the need to revise and reduce the number of projects keeping in view the CAS score card, formulation of inter-divisional/ multidisciplinary project with separate service and research component. Recommendations that emerged during presentations and subsequent discussions are presented at the end including general recommendations to be followed by all concerned. Presentations by Head of Divisions/Units/Regional Stations followed by the respective PIs of the project were made as follows.

DIVISION OF PLANT EXPLORATION AND COLLECTION: Programme: 1. Exploration for collection of

germplasm of agri-horticultural crops, maintenance of herbarium and biosystematic and ethno-botanical studies

D C Bhandari PGR/PGC-BUR-DEL-01.00

Projects 1.1 Exploration for collection of

genetic resources of agricultural crops and their wild relatives

D C Bhandari, KC Bhatt, Rakesh Srivastava, Anjula Pandey, DP Semwal and NS Panwar

PGR/PGC-BUR-DEL-01.01

1. 2 Exploration for collection of genetic resources of horticultural

Rakesh Srivastava, ER Nayar, SK Malik, and Rakesh Singh


crops and their wild relatives. 1. 3 Exploration for collection of

medicinal and aromatic plant diversity from different phyto-geographical regions.

KC Bhatt, RC Misra, DP Semwal, CS Raghav (upto 15.11.2011), Rakesh Singh and NS Panwar


1.4

National Herbarium of Cultivated Plants (NHCP), establishment , maintenance , build-up and taxonomic studies on Indian crop plants

E Roshini Nayar, Anjula Pandey, K Pradheep and Rita Gupta


1.5 Genetic resources and systematic study of Alliaceae in India: Allium

Anjula Pandey, K Pradheep, K S Negi and Rita Gupta


1.6 Genetic resources and systematic studies of Cucurbitaceae in India: Trichosanthes

K Pradheep, K C Bhatt, DR Pani and Rakesh Singh


Progress/Achievements:

• A total of 46 explorations including 18 under NEH special drive and 4 under NICRA project were carried out in 21 states in collaborative mode with ICAR crop-based institutes (IIHR, IIVR, CRRI, CICR, VPKAS, CITH, DMR, DRMR, DOGR, DRR, ICAR Goa and ICAR NEH)/ SAUs/ NGO and 34 KVKs in NEH region.

• A total of 3278 accessions of different agri-horticultural crops comprising 2779 accessions of cultivated and 499 of wild species (15.2%) including wild relatives of crop plants and other economic/potential species were collected. The crop-group wise details are: cereals & pseudo-cereals (817), millets & minor millets (238), pulses (571), oilseeds (125), vegetables (1035), fruits (184), M & AP (226) and others (82).

• A total of 289 herbarium specimens, 34 seed samples and 35 economic products were added to the National Herbarium of Cultivated Plants (NHCP). Identification services were given to 167 indenters.

• Systematic studies were continued in Allium (33 samples of 12 taxa) and Trichosanthes

(70 samples of 6 taxa).

• Gap analysis work for mapping of collected diversity was completed in Brassica spp. (6) and vegetables (over 100) and initiated in maize. Facilities were build-up with work station/ computers, GIS software and other accessories to facilitate the mapping and diversity analysis of collected germplasm

GERMPLASM EXCHANGE UNIT:

Programme 6.1 Exchange of Plant Genetic

Resources with foreign countries (import/ export) and national supplies of the Plant Genetic Resource and related information to the scientists /users in the country.

Arjun Lal

PGR/GEX–BUR -DEL-01.00

Projects

6.1.1 Import, export and inland supply of PGR in Cereals, Pseudo cereals, Oilseeds, Fibers, Medicinal & Aromatic Plants, Millets, Sugar yielding crops and exchange of related information to the scientists and users in the country .

Vandana Tyagi, Pratibha Brahmi, Anitha Pedapati (on study leave), S P Singh, Surender Singh

PGR/GEX–BUR-DEL-01.01

6.1.2 Import, export and inland supply of PGR in Vegetables, Forages, Under Utilized Plants, Ornamentals, Spices and Condiments, Plantation crops and exchange of related information to the scientists and users in the country.

S K Yadav, Nidhi M Verma, Anitha Pedapati (on study leave), S P Singh, Surender Singh

PGR/ GEX– BUR-DEL-01.03

6.1.3 Import, export and inland supply of PGR in Fruits, Grain Legumes, Tubers, Narcotics and Beverages and exchange of related information to the scientists and users in the country.

Nidhi M Verma, S K Yadav, S P Singh, Surender Singh


6.1.4

Documentation and dissemination of information on germplasm both imported and collected in the form of Plant Germplasm Reporter and preparation of Crop Inventories.

Pratibha Brahmi, Vandana Tyagi, Nidhi M Verma, S K Yadav, Anitha Pedapati (on study leave), S.P. Singh, Surender Singh


6.1.5

Survey and assembly of literature on Plant Genetic Resources and its documentation for procurement of elite/trait specific germplasm.

Arjun Lal, Vandana Tyagi, Nidhi M Verma, S K Yadav , Anita Pedapati (on study leave), S P Singh, Surender Singh


Progress/ Achievements:

A total of 36,239 samples comprising 35,572 accessions were imported as germplasm. A total of 1,00,736 samples of 7,044 accessions were imported as trial materials. A total of 1672 accessions of different crops were exported. A total of 769 Import Permits were issued

PLANT QUARANTINE DIVISION: Programme

5. 1 Quarantine Processing of Germplasm Under Exchange and Supportive Research

P.C. Agarwal PGR/PQR-BUR-DEL-01.00

Projects

5.1.1. Quarantine processing of Germplasm for Joint inspection

P.C. Agarwal, Usha Dev, Kavita Gupta, D.B. Parakh, Moolchand Singh, Charan Singh, Dinesh Chand, Ashok Kumar Maurya

PGR/PQR-BUR-DEL-01.01

5.1.2. Post-entry Quarantine Processing of Imported Germplasm

P.C. Agarwal,, Arjun Lal, Beche Lal (upto 31.07.2011), D.B. Parakh, V. Celia Chalam, Charan Singh and Ashok Kumar Maurya


5.1.3. Quarantine Processing of Imported Transgenic Germplasm and Supportive Research

Manju Lata Kapur, Z Khan, Baleshwar Singh, Shashi Bhalla, Gurender Jit Randhawa, V. Celia Chalam and Ashok Kumaa Maurya


5.1.4. Detection and Identification of Insect and Mite Pests in Quarantine and Supportive Research

Kavita Gupta, Beche Lal (upto 31.07.2011), Manju Lata Kapur, Shashi Bhalla and Charan Singh


5.1.5. Detection and Identification of Nematode pests in quarantine and Supportive Research

Arjun Lal and Z Khan PGR/PQR-BUR-DEL-01.05

5.1.6. Detection and Identification of Fungi and Bacteria in quarantine and Supportive Research

Usha Dev, P.C. Agarwal, Baleshwar Singh, J Akhtar, A Kandan and Dinesh Chand


5.1.7. Detection and Identification of Viruses in Quarantine and Supportive Research

V. Celia Chalam, D.B. Parakh, and Ashok Kumar Maurya


Resource generation was Rs 65,54,250/- during the period.


• A total of 1,23,088 samples (1,20,628 imported and 2,460 meant for export) were processed for quarantine clearance. In case of imported samples, 3,224 were found infested/ infected with various pests (insects/ mites- 444, nematodes- 1,940, fungi/ bacteria- 744, viruses-27, weeds- 69). Among various pests intercepted, 2 insects, one nematode and 6 weeds are not yet reported from India. Of the 3,525 infested/ infected/ contaminated samples, 3,191 samples were salvaged using various physico-chemical treatments. 19,774 samples of various crops were given prophylactic treatments viz., fumigation (4,010), hot water (15,267), pesticidal dip/spray (497), and 10% tri-sodium orthophosphate (1,102). A total of 2,460

5.1.8. Quarantine Treatments for Disinfestation of Germplasm Under Exchange Against Insect and Mite Pests and Supportive Research

Shashi Bhalla, Beche Lal (upto 31.07.2011), Manju Lata Kapur, Kavita Gupta, T V Prasad and Charan Singh


5.1.9. Quarantine Treatments for Disinfestation of Germplasm Under Exchange Against Nematodes pests and Supportive Research

Z Khan, and Arjun Lal PGR/PQR-BUR-DEL-01.09

5.1.10. Quarantine Treatments for Disinfection of Germplasm Under Exchange Against Pathogenic Fungi and Bacteria and Supportive Research

Baleshwar Singh, P.C. Agarwal, Usha Dev, J Akhtar, A Kandan and Dinesh Chand


5.1.11. Seed Health Testing for Conservation of Indigenous Germplasm Free From Pests

Beche Lal (upto 31.07.2011), Arjun Lal, Usha Dev, Manju Lata Kapur, Baleshwar Singh, Shashi Bhalla, Veena Gupta, Sushil Pandey, J Akhtar, A Kandan, Smita Jain, Charan Singh, Ashok Kumar Maurya and Dinesh Chand


5.1.12. Detection of Viruses in in vitro Cultures of Germplasm Meant for Conservation

D.B. Parakh, V. Celia Chalam, Sandhya Gupta and Ashok Kumar Maurya


5.1.13. Identification of intercepted weeds and supportive research in quarantine

Mool Chand Singh, Anjula Pandey and Dinesh Chand


samples were processed for export, 181 infested/ infected samples were salvaged and fifteen Phytosanitary Certificates were issued.

• Fifty six samples of exotic germplasm of different legume crops imported from different countries/ sources were grown in post-entry quarantine (PEQ) greenhouses for virus-indexing. Post entry quarantine inspection at indentor’s site of 214 legume samples (released on undertaking) was undertaken. Seventy one samples comprising 63 accessions were tested by variants of ELISA using antisera to 26 viruses supplemented with electron microscopy. Eight viruses were intercepted. Harvest from virus-free plants was released to the indenters. Also 4,673 samples of wheat, barley and triticale comprising trials, germplasm and registered material were processed in PEQN.

• Nine hundred and fifty four samples of imported transgenic planting material were processed for quarantine clearance. Intercepted pests included fungi-Drechslera oryzae, D. sorokiniana and Fusarium verticilloides in paddy; fungus -F. verticilloides, and viruses (which are not yet reported from India)- Barley stripe mosaic virus, Maize chlorotic mottle virus and Wheat streak mosaic virus in maize. Maize and rice samples were grown in Containment facility to observe the expression of seed-transmitted pests. Absence of terminator gene was ensured and all samples were salvaged prior to release. Thirteen post-entry quarantine inspections were undertaken.

• A sum of 12,547 samples of various crops was processed for seed health testing for pest-free conservation. A total of 3,211 samples were found infested/ infected / contaminated (insects =1,602, fungi/ bacteria= 1,609 and weeds = 17samples). Four hundred eighteen samples were rejected.

• Teaching/ HRD: Taught three courses of PG School, IARI (PGR-502,-509 and PP-520). Three (Ph.D.=2 and one M.Sc. =1) thesis were submitted. Guiding one M.Sc. student. Guided seven students for 2-6 months project work. Training Course Organized=1

• Publications: Research Papers=19, Edited Book=1, Training Manuals=2, Chapters in Books/ Proceedings=10, Chapters in Training Manuals=30, Technical Bulletins=3, Technical/ Popular Articles=7, Presentations in Conferences/ Seminars/ Symposia/ Workshops=20, Partial ribosomal RNA gene sequences of fungi submitted to NCBI GenBank=3, CDs=3.

GERMPLASM EVALUATION DIVISION:

Programme

2.1 Characterization, Evaluation, Maintenance, Regeneration and Documentation of Germplasm Resources of Various Crops.

M. Dutta PGR/GEV-BUR-DEL-01.00

Projects

2.1.1. Characterization, Evaluation, Maintenance, Regeneration and Documentation of Germplasm Resources of Wheat, Barley and Triticale

B S Phogat, T.P. Singh, M C Singh, Sundeep Kumar, Sandeep Kumar, Y.S. Rathi

PGR/GEV-BUR-DEL-01.01

2.1.2. Characterization, evaluation, maintenance, regeneration and documentation of germplasm resources of maize

Ashok Kumar, Jyoti Kumari, Sandeep Kumar, Harender Singh and R K Sharma


2.1.3. Characterization, evaluation, maintenance, regeneration and documentation of germplasm resources of pulse crops

N.K. Gautam, Sangita Yadav, Mohar Singh, A. Roy, Z. Khan, T.V. Prasad, OP Dahiya and Babu Ram


2.1.4. Characterization, evaluation, maintenance, regeneration and documentation of Germplasm Resources of oil seeds

Ranbir Singh, M. K. Bag, Sangita Yadav, Rashmi Yadav and Poonam Suneja


2.1.5. Characterization, evaluation, maintenance, regeneration and documentation of germplasm resources of rabi vegetable crops

Pragya, K.K. Gangopadhyay, A. Roy, Z. Khan, T.V. Prasad, M. Arivalagan, M. K. Bag and B. L. Meena


2.1.6. Characterization, evaluation, maintenance, regeneration and documentation of germplasm resources of kharif vegetable crops

K. K. Gangopadhyay, Pragya, S.K Yadav, A. Roy, Z. Khan, T.V. Prasad, M. K. Bag, M. Arivalagan and B.L. Meena


2.1.7. Characterization, evaluation, maintenance, regeneration and documentation of germplasm resources of medicinal plants

Ashok Kumar, S.K. Pareek (upto 31.07.2011), Archana Raina, Arivalagan, Harender Singh and Ombir Singh


2.1.8. Biochemical evaluation of germplasm resources of various field crops

Sangita Yadav, Rakesh Bhardwaj, Sandeep Kumar, Ranbir Singh, N.K. Gautam, Zakir Hussain and Poonam Suneja


2.1.9. Characterization, evaluation, maintenance, regeneration and documentation of germplasm resources of under-utilized crops

B.S. Phogat, Rashmi Yadav, Hanuman Lal and Ranbir Singh


2.1.10. Studies on Statistical Techniques for Efficient Management of Plant Genetic Resources

Hanuman Lal PGR/GEV-BUR-DEL-01.10

2.1.11. Genetic Resources Information Programme

Hanuman Lal, Madhu Bala and Rajiv Gambhir


2.1.12. Characterization, Evaluation, Maintenance, Regeneration and Documentation of Germplasm Resources of Aromatic Plant

Archana Raina, S.K. Pareek (upto 31.07.2011), Ashok Kumar and Ombir Singh


2.1.13. Development of Core set in Brinjal (Solanum melongena L.)

K. K. Gangopadhyay, S. K. Yadav, Pragya, and I.S. Bisht


2.1.14. Prebreeding and genetic enhancement in lentil and chickpea genetic resources

Mohar Singh, T.P.Singh, Jyoti Kumari, M. Dutta , I. S. Bisht, and Mukesh Rana


2.1.15. Evaluation for abiotic stress (terminal heat tolerance) in wheat germplasm

T.P. Singh, Mohar Singh, Rashmi Yadav, Rakesh Bhardwaj, and Jyoti Kumari


2.1.16. Standardization of agro-techniques of elite medicinal plants (Giloe and Shatavar)

Moolchand Singh, B.S. Phogat, S.K. Pareek (upto 31.07.2011), Archana Raina, M. Arivalagan and B. S. Panwar



• A total of 5888 accessions of various agri-horticultural crops comprising cereals (1504), millets (235), pulses (2123), oilseeds (112), vegetables (1286), underutilized crops (493), and medicinal and aromatic plants (135) were characterized and evaluated.

• A total of 1660 accessions of various agri-horticultural crops were regenerated and multiplied.

• 4503 accessions of wheat, barley and triticale were evaluated in Post Entry Quarantine Nursery (PEQN).

• A total of 2376 accessions of various crops namely, pearl millet (223), rapeseed-mustard (713), black gram (344), chickpea (90), lentil (300), okra (200), tomato (582), brinjal

(200) were evaluated for important biotic stresses (insects/pests and diseases). Under abiotic stress, 240 accessions of wheat were evaluated for terminal heat tolerance.

• In bio-chemical evaluation, a total of 1112 accessions of different crops were analyzed for oil content, fatty acid profile, protein, essential amino acids, phenols, sugars, anti-nutritional factors, antioxidants and minerals etc.

• Under phyto-chemical evaluation 465 samples of different medicinal and aromatic plants were analyzed for their active compounds.

• Sixty wild accessions of Lens species and fifty wild accessions of four Cicer species were characterized and evaluated for various agro- morphological traits.

• Seven inter-specific crosses have been attempted between cultivated and wild Lens species for widening the genetic base.

• Under multi-location evaluation (MLE), a total of 3814 accessions of various crops namely of wheat (1100), rice (900), maize (200), mustard (240), brinjal (200), okra (200), chickpea (320), pigeon pea (340), lentil (300) and Tinospora (14) were characterized and evaluated for agro-morphological, important biotic, abiotic and quality parameters.

• Four Germplasm Advisory Committee (GAC) Meetings on various crop groups were organized.

• Germplasm Field Days on wheat at IARI Regional Station, Wellington, on Maize & Okra at NBPGR New Area, New Delhi and on Chickpea at MPKV, Rahuri were organized in which scientists from ICAR institutes and SAUs participated.

• Wheat Germplasm Week was organized at four locations comprising NBPGR Experimental Farm, Issapur, NBPGR Post Entry Quarantine Nursery (PEQN), New Delhi, NBPGR New Area Farm (IARI), New Delhi and at CCS HAU, Hisar.

• A Model Training Course sponsored by Directorate of Extension, Ministry of Agriculture, Department of Agriculture & Cooperation, GoI, New Delhi on Role of Underutilized and Neglected Plant Genetic Resources in Health and Nutritional Security was organized.

• A total of 3,694 accessions of various agri-horticultural crops were supplied to 70 indenters for their utilization in crop improvement.

• A total of 37,046 accessions of various agri-horticultural crops are being maintained under medium term storage (MTS).

• Under documentation of information, two Crop Catalogues and two Reports on Germplasm Characterization and Evaluation were published.

GERMPLASM CONSERVATION DIVISION: Programme 3.1 Ex Situ Conservation of Plant

Genetic Resources of Agricultural and Horticultural Crops Using Conventional Methods

R.K.Tyagi PGR/GCN-BUR-DEL-01.00

Projects

3.1.1 Management of Information and National Germplasm Conservation Network

R.K.Tyagi, Sunil Archak , Anjali Kak, B. P. Dahiya, Rajvir Singh, Rita Rani, Smita Jain and Rajiv Gambhir

PGR/GCN-BUR-DEL-01.01

3.1.2 Conservation of Grain Legume Germplasm using Conventional Seed Storage Methods

Neeta Singh and Chitra Pandey


3.1.3 Conservation of Paddy Germplasm using Conventional Seed Storage Methods

Kalyani Srinivasan, R. K. Tyagi, Sherry Rachel Jacob, and A D Sharma


3.1.4 Conservation of Oilseed and Fruit Crops Germplasm using Conventional Seed Storage Methods

J. Radhamani and Neeta Singh,


3.1.5 Conservation of Germplasm of Cereals, other than Paddy and Agro-Forestry species using Conventional Seed Storage Methods

Sherry Rachel Jacob, Kalyani Srinivasan, J. Radhamani, and Manju Uprety (upto 30.06.2011)


3.1.6 Conservation of Germplasm of Pseudo-cereals, Medicinal and Aromatic, Narcotics and Species using Conventional Seed Storage Methods

Veena Gupta and Anjali Kak


3.1.7 Conservation of Millets Germplasm using Conventional Seed Storage Methods

Sushil Pandey and Chitra Pandey


3.1.8 Conservation of Fibre and Forage Anjali Kak and Veena PGR/GCN-BUR-DEL-


During the period of report a total of 5,244 germplasm accessions belonging to various crops were processed and transferred to National Genebank for long-term conservation raising the number of holdings to 3,90,092. These comprised cereals (2,461), millets and forages (524), pseudo-cereals (128), grain legumes (357), oilseeds (535), fibre crops (305), vegetables (425), medicinal, aromatic plants & narcotics (269) and spices & condiments (240).

A total of 151 released varieties belonging to 46 crops were received, raising the number of varieties conserved in long term storage to 3,995. These comprised cereals (40), millets and forages (24), grain legumes (29), oilseeds (21), fibre crops (8), vegetables (17) and others (13).

During this period 88 unique accessions belonging to 11 different crop groups were approved for the registration, raising the total registered germplasm to 1,030 belonging to 183 species.

A total of 8,413 representative accessions from seed lots of various crops which have been in long-term storage for 10 or more years, were monitored for their seed viability, seed quantity and seed health. These comprised cereals (4,885), millets and forages (968), pseudo-cereals (138), grain legumes (505), oilseeds (870), fibre crops (822), vegetables (125), and spices & condiments (100). The results indicate that the entire monitored crop species maintained the final viability closer to their initial viability confirming to gene-bank standards except few accessions of paddy, maize and oilseeds. By now 37,039 accessions of various crops have been monitored and developmental deterioration in seed-viability, seed health and quantity is noticed in cereals and oilseeds.

A total of 12,891 seed samples of exotic germplasm were stored as voucher samples in medium-term storage. This consisted of 7,981 samples of cereals, 2,645 of grain legumes,

Germplasm using Conventional Seed Storage Methods

Gupta 01.08

3.1.9 Conservation of Vegetable Germplasm using Conventional Seed Storage Methods

Chitra Pandey, Neeta Singh and Sushil Pandey


3.1.10 Investigating Seed Dormancy, Seed Storage Behaviour, Physiological and Biochemical Changes during Storage.

J. Radhamani, Kalyani Srinivasan, Neeta Singh, Veena Gupta, Chitra Pandey, Sushil Pandey, Anjali Kak and Sherry Rachel Jacob


118 of oilseeds, 861 of vegetables, 558 of fibre and forages, 8 of medicinal and aromatic plants and 487 of spices.

A total of 45,647 accessions were supplied to different partners for regeneration, multiplication, species identification and research use. In addition a set of around 21,000 wheat accessions were distributed to IARI-Wellington, HAU-Hissar, Issapur Farm-NBPGR each, for characterization and detailed evaluation.

Supportive research studies were undertaken on the acquisition, loss and re-establishment of desiccation tolerance in wheat, assessment of viability in conserved germplasm of Andrographis paniculata, mapping of seed-borne micro-flora of sorghum and pearl millet conserved in NGB from different states and identification of duplicates using DNA Markers in Brinjal were undertaken. Studies have also been initiated in collaboration with DRDO with 19 crops and 23 varieties to study the suitability of perma frost facility on conservation of germplasm.

TISSUE CULTURE AND CRYOPRESERVATION UNIT:

Programme 1

4.1 Ex Situ Conservation of Plant Genetic Resources of Vegetatively Propagated Crops Using In Vitro and Cryopreservation Techniques

R.K. Tyagi PGR/TCCU-BUR-DEL-01.00

Projects

4.1.1 In Vitro Conservation of Tuber Crops with Special Reference to Sweet Potato, Yams and Taro

Neelam Sharma, Zakir Hussain, and DK Nerwal

PGR/TCCU-BUR-DEL-01.01

4.1.2 In Vitro Conservation of Species, Plantation and New Industrial Crops

Anju Jain, R.K. Tyagi, and R.P. Yadav

PGR/ TCCU–BUR-DEL-01.02

4.1.3 In Vitro Conservation of Bulbous and Ornamental Crops

Ruchira Pandey and Neelam Sharma

PGR/ TCCU–BUR-DEL-01.03

4.1.4 In Vitro Conservation of Medicinal and Aromatic Plants with Special Reference to Rare and Endangered Species

Neelam Sharma and Ruchira Pandey

PGR/TCCU–BUR-DEL-01.04

4.1.5 In Vitro Conservation of Tropical Fruit Crops Anuradha Agarwal and R.K. Tyagi


4.1.6 In Vitro Conservation of Temperate and Minor Tropical Fruit Crops.

Sandhya Gupta and K. Pradheep


4.1.7 Studies on Genetic Stability of In Vitro Conserved and Cryo-preserved Germplasm

Zakir Hussain, R.K. Tyagi and DK Nerwal


Programme 2 4.2 Ex situ Conservation of Plant Genetic

Resources of Agricultural and Horticultural Crops Using Cryopreservation of Seeds, dormant buds and pollen

Rekha Chaudhury PGR/TCCU-BUR-DEL-02.00

Projects 4.2.1 Cryopreservation of Non-orthodox and

Orthodox Seed Species in Various Forms using Standard Protocols

Rekha Chaudhury and S.K. Malik


4.2.2 Investigating Desiccation and Freezing Tolerance in Non-orthodox Seed Species, Dormant Buds and Pollen for Cryopreservation.

S.K. Malik, Rekha Chaudhury and Z. Abraham



• A total of 140 accessions procured for in vitro establishment, and 40 new accessions of

apple, taro, yams and medicinal plants were successfully introduced in vitro. About 40,000 cultures of 2,082 accessions were maintained in the In Vitro Genebank by periodic subculturing.

• Cryobanking of in vitro germplasm was done in Musa germplasm for 16 new accessions, three accessions of Dioscorea (2 replicates each) and initiated in 3 accessions of garlic (one replicate each). Cryoprotocols refined in apricot using droplet vitrification, Allium hookerii using vitrification technique. Application of in vitro cryopreservation protocol, developed thus far, to 25 accessions of A. sativum procured from Bhowali.

• Genetic stability studies carried out in in vitro conserved and cryopreserved accessions of Allium spp., Colocasia esculenta and mulberry using SSR primers and in banana by morphological traits. No significant differences were observed in the regenerated plantlets and their respective controls.

• As follow up of recommendation of the Brainstorming on ‘Management of Horticultural Crop Genetic Resources’ held at NBPGR on April 21, 2009, 274 accessions of sweet potato, taro and yams were suitably multiplied and shifted to CTCRI, Trivandrum. A total of 85 accessions comprising turmeric (55) and ginger (30) multiplied for onward shifting to IISR, Calicut. Accessions of banana (34), Picrorhiza sp. (1) and Bacopa sp. (1) were supplied as in vitro cultures to various institutes for field evaluation and other research work.

• A total of 271 accessions assembled by 3 explorations and other collaborations mainly of wild spp., intermediate seed species of fruits, nuts, spices, and industrial crops, dormant buds and successful cryopreservation achieved in 238 accessions as seeds, embryonic axes and dormant buds totalling to 9,893 accessions in cryogenebank. Two-step freezing, Vitrification and Encapsulation-dehydration attempted in winter buds of Morus spp., Prunus amygdalus and Juglans regia. Genetic stability tested in cryostored mulberry buds.

• Desiccation and freezing sensitivity investigated in Manilkara hexandra, Glycosmis pentaphylla, several Citrus species viz., C. karna, C. pseudolimon, C. megaloxycarpa, C. pati-jora (citron), C. ichangensis and C. limonia. Retesting of cryostored accessions (20) of dormant buds and (more than 150) of seeds both of orthodox and non-orthodox was done.

• Sixth International training course under the Centre of Excellence programme of ICAR-Bioversity International on ‘In Vitro and Cryopreservation Techniques for Conservation of Plant Genetic Resources’ was organized by NBPGR, Bioversity International, South Asia Office, APCoAB New Delhi and Royal Botanic Gardens, Kew from 14th to 26th Nov, 2011 where 11 participants from 6 countries namely Iran, Ghana, Nigeria, Taiwan, Sri Lanka and South Africa and India participated.

NRC on DNA FINGERPRINTING:

Programme 1

8.1 Development of genomic tools for the enhanced utilization of PGRs

KV Bhat PGR/DFP-BUR-DEL-01.00

Projects

8.1.1 Development of genomic tools for discovery and validation of genes of economic importance for enhancing the use of plant genetic resources of pulses and oilseeds.

KV Bhat, MK Rana, Yasin Jeshima, Rajesh Kumar, J Radhamani, M Latha, Trichur; M. A. Nizar, Akola; Ranbir Singh and Mohar Singh, GED

PGR/DFP-BUR-DEL-01.01

8.1.2 Development of genomic tools for enhanced utilization of fiber and forage crops

MK Rana, Amit Kumar Singh, T. K. Mondal and J C Rana, Shimla


8.1.3 Development of genomic tools for enhanced utilization of cereal and millets

Lalit Arya, M. Verma, Chet Ram, M Yadav, Sundeep Kumar, D Saha, T K Mondal, Amit Kumar Singh, B S Phogat, Sangita Yadav, GED, N K Dwivedi, J C Rana, S. Pandrawada, Hyderabad and Firoze Hussain, IARI


8.1.4 Development of genomic tools for enhanced utilization of cucurbitaceous crops

M Verma, L Arya, Chet Ram, D Saha, Monendra Grover, and Rakesh Bharadwaj, GED


8.1.5 Development of genomic tools for enhanced utilization of medicinal and aromatic plants.

Rakesh Singh, Amit Kumar Singh, S. Marla, Sundeep Kumar, N S Pawar, A. Raina, and Ashok Kumar, GED


8.1.6 Development of genomic tools for enhanced utilization of under-utilized crops

S. Archak, A B Gaikwad, AK Trivedi, Bhowali; JC Rana, Shimla; and N K Dwevedi (upto 08.02.2012) and Om Vir Singh (wef 09.02.2012), Jodhpur;


8.1.7 Development of genomic tools for enhanced utilization of horticultural crops

AB Gaikwad, KS Negi, Bhowali; and Joseph John, Thrissur


Programme 2

8.2 Establishment and maintenance of National Genomic Resources Repository

Sunil Archak PGR/DFP-BUR-DEL-02.00

Projects

8.2.1 Establishment and maintenance of National Genomic Resources Repository

S Archak, KV Bhat GJ Randhawa, M Yadav, AB Gaikwad, MK Rana, Rakesh Singh, M Verma, Lalit Arya, Sundeep Kumar, T K Mandal, D Saha, Rajkumar and Rajesh Kumar, Amit Kumar Singh, R Parimalan, Chet Ram, Yasin Jeshima and R. Chaudhury, TCCU


8.2.2 Documentation and maintenance of data base for National Genomic Resources Repository

Soma S. Marla, R. C. Agrawal, Madhu Bala, Monendra Grover, and Dayashankar


Programme 3

8.3 Development of diagnostics for transgene detection and biosafety assessment in crop plants

GJ Randhawa PGR/DFP-BUR-DEL-03.00

Projects

8.3.1 Molecular diagnosis of GM crops GJ Randhawa, S. Archak and R Parimalan


8.3.2 Screening ex-situ germplasm collections for adventitious presence of transgenes

GJ Randhawa, S. Archak and R Parimalan


Programme 4

8.4 Exploitation of molecular genetic tools for species delineation and genetic erosion studies in agri-horticultural

MC Yadav PGR/DFP-BUR-DEL-04.00

crops 8.4.1 Development of DNA bar-codes

for identification of wild relatives and molecular phylogeny in important crops

MC Yadav, Rakesh Singh, S Rajkumar, K. Yasin Jeshima, and D. R. Pani, Cuttack


8.4.2 Monitoring temporal variation in genetic diversity for devising effective PGR management strategies in safflower

S. Rajkumar, MC Yadav, K. Yasin Jeshima, J. Radhamani and M. A. Nizar, Akola



• Eleven polymorphic functional markers (EST-SSRs) were used for genetic diversity analysis in 12 germplasm lines of finger millet of Indian and African origin for genetic variability studies. Genetic characterization of Morinda tomentosa accessions were analyzed using 21 ISSR primers. Seventy ISSR markers used for characterization of collected accessions of Luffa species. Molecular diversity among 24 released varieties and germplasm lines of wheat have been demonstrated with 32 identified SSR markers having high PIC value. A total of 391 cotton samples were fingerprinted using 25 genome-wide microsatellite loci. In 94 accessions of Linum microsatellite fingerprinting was carried out. Studies on genetic variability and characterization of germplasm of Jatropha curcas, 285 selected accessions were analysed using AFLP and SSR markers. In addition 18 RAPD and 24 SRAP primers were used to analyse 37 Pongamia accessions. In wheat, a 237 promising germplasm lines showed near immunity for leaf rust resistance in nine different locations across the climatic zones in India. A new program was initiated to develop mapping populations for heat and drought tolerance in wheat during 2010 summer at IARIRS, Wellington, Tamil Nadu and NBPGR, New Delhi.

• Novel promoter region targeted marker CBDP (CAAT box derived polymorphism) and Start Codon Targeted (SCoT) polymorphism markers were developed and demonstrated their utilization in cultivars of Corchorus capsularis and Corchorus olitorius. SRAP (Sequence-Related Amplified Polymorphic) marker was also standardized in jute. Genomic SSR markers have been developed in finger millet. Ten Resistance gene analogue polymorphism (RGAP) markers were demonstrated in finger millet to produce polymorphic profiles in 32 finger millet accessions. Sixty SSR markers have been demonstrated for transferability from cucumber to characterize 40 bottle gourd germplasm lines. Eight SSR enriched library has been developed each for Giloe and Andrographis using different combination of biotin labeled repeat sequences. Ninety eight microsatellite primers in jute were custom synthesized and screened for amplification in a representative panel of eight genotypes. Twenty nine new microsatellite loci have been identified through genomic library enrichment and sequencing for identification of microsatellite markers in bittergourd.

• Cloning of a novel cold tolerance gene COR14 from white clover (Trifolium repens) has been carried out. A carotenoid pathway gene, phytoene synthase 1, was amplified (750bp

amplicons) and sequenced from red pulp black seeded watermelon line DRB-669 using degenerate primers from conserved region of psy1 gene. Sixteen Resistance gene candidates (RGCs) has been isolated and characterized from ToLCND Virus tolerant sponge gourd genotype and submitted to GeneBank. A Carbonic anahydrase gene has been amplified and characterized from the young leaves of cowpea cv. PUSA Kamal. Allelic variations of the CA gene have been demonstrated in cowpea genotypes. In mothbean SSH library has been developed for moisture stress. Species relationship studies have been conducted in Luffa spp. using selected genomic DNA regions.

• Multiplex-PCR based diagnostic tools have been developed for GM-maize events. Event-

specific Real-time PCR protocols have also been developed in Bt Brinjal and Bt cabbage. A real time PCR-based diagnostics of GM event in imported transgenic planting materials has been conducted. DNA fingerprinting services has been provided to agencies and resources were generated through the service. A sponsored training programme on “Forest Biotechnology” for senior scientists of Indian Council of Forestry Research and Education, Dehradun was organized.

PGR POLICY UNIT:

Programme

7.1 PGR Management Policy and Back-up Research

Pratibha Brahmi PGR/PPU-BUR-DEL-01.01

Projects

7.1.1 PGR Management and Related Policy Issues

Pratibha Brahmi, and Vandana Tyagi

PGR/PPU-BUR-DEL-01.01

7.1.2 Policy Issues Related to Biosecurity P C Agrawal, Pratibha Brahmi, and Kavita Gupta

PGR/PPU-BUR-DEL-01.02

• Progress/ Achievements: • 68 applications of extant-notified and new varieties of ICAR- SAU system for the crops

notified by PPV& FR Authority, were scrutinized, documented and submitted to PPV &FRA.

• Two meetings of the Institute Technology Management Committee (ITMC) were organized and MoU for transfer of technology, “PCR based detection protocols for GM crops” to Microchan Sillikar, Mumbai.

• Inputs provided to Department of Agriculture and Cooperation, MoA for Plant Quarantine (Regulation for Import into India) Order 2003.

• Input to APAARI/ APCoAB, MoEF, MoCI, ICAR, NDMA, Ministry of Defence, MEA for various bio-safety and quarantine related issues.

AGRICULTURAL KNOWLEDGE MANAGEMENT UNIT: Programme

1.1 Genetic Resources Information Programme

Sunil Archak PGR/ARIS - BUR-DEL-01.00

Project

1.1.1 Molecular Data Analysis Software

Madhu Bala Priyadarshi

PGR/ ARIS - BUR-DEL-01.01

1.1.2 Bioinformatics Software Portal Madhu Bala Priyadarshi and Soma S. Marla

PGR/ ARIS - BUR-DEL-01.02


• Search engine for registered germplasm developed

• Search engine for multilocation evaluation developed

• Data entry as below made: Passport Information 5,807 Germplasm Exchange and Quarantine Information 40,420 Genebank Information 7,536 Germplasm Evaluation Information 206 crops 506 exp.

• LAN, internet, email services were maintained

NETWORK PROJECT on UNDER-UTILIZED CROPS: Project All India Coordinated Research Project on Underutilized Crops

D C Bhandari and Hanuman Lal


• A total of 161 experiments were allotted during 2011-12 which included germplasm

evaluation (59), breeding (55), agronomic (24) and quality aspects (23). These were allotted at twenty three locations in different agro-climatic zones of the country. Out of these, 140 trials were carried out.

• Promising genotypes based on three years data:VRB-3 in rice bean,HB-516 in faba bean and SKNK-0806 in Kalingda

• About 1038 accessions in different crops, some of them tested at more than one location, were evaluated at twenty locations during 2011-12

• Promising genotypes for Protein (IC038430:13.60%, PRA-3: 12.40%); Oil (IC038498:

10.30%, Annapurna: 8.40%); Phenols (IC038384: 0.051%, Annapurna: 0.058%) in grain amaranth, Protein (LRB-467: 20.8%, PRR-1: 19.7%); Tannin (LRB-461: 576 mg/100g, PRR-2: 608 mg/100g); Cooking time (LRB-472: 47 min., PRR-1: 52 min.); in rice bean, Crude protein (EC340266: 23.2%, Totru Local: 23.6%); Ca (EC340256: 369 mg/100g, Totru Local: 306 mg/100g);Iron (EC340257: 9.2 mg/100g, HPU-51: 8.0 mg/100g); Zn (EC340279: 4.1 mg/100g, Totru Local: 3.0 mg/100g) in adzuki bean, Crude protein (IC202268:13.6%, VL-7 & Sangla-B-126: 13.1%);Ca (EC218742: 99.8 mg/100g, PRB-1: 95.2 mg/100g;Iron (IC204088: 8.8 mg/100g, Sangla-B-5: 7.0 mg/100g);Zn (EC125397: 4.4 mg/100g, Sangla-B-118: 4.0 mg/100g) in buckwheat.

• Standardization of different recipes for value addition: Gulgulae of wheat supplemented value added products, Mithdu,Pinni,nugguets of rice bean based value added products and Mathri, Sevian of Adzuki bean based value added products.

• A total of sixteen agronomic experiments were formulated to be conducted at nine locations in 24 trials. These comprised of five studies on amaranth, one each on buckwheat, jatropha and kalingada, five on rice bean and three on underutilized crops in general during Rabi 2010-11 and Kharif 2011. Out of 24 trials allotted, results of 19 were received.

REGIONAL STATION, AKOLA:

Programme

1.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources in the Central Indian Plains

M. Abdul Nizar PGR/GEV-BUR-AKO-01.00

Projects

1.1.1 Augmentation, characterization, evaluation, maintenance, conservation, documentation and distribution of genetic resources of Pulses (Pigeon pea and Chick pea), Vegetables (Winged bean, Amaranth and Okra) and Under Utilized

M. A. Nizar and N. Dikshit

PGR/GEV-BUR-AKO-01.01

crops. 1.1.2 Augmentation, Characterization,

Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Oil Seeds (Sesame, Niger, Castor, Ground nut, Safflower, Soybean and Linseed) and Millets and Minor Millet.

N. Dikshit and M. A. Nizar

PGR/GEV-BUR-AKO-01.02


• Exploration & collection: Undertook two exploration and collection missions in the

Chattisgarh and adjoining Uttar Pradesh under NICRA and West Tripura and South Tripura districts in the state of Tripura under NEH programme during the reporting period. A total of 101 accessions of germplasm comprising Oruza nivara Sharma et Shastry (17), Oryza rufipogon Griff. (16) and Oryza sativa L.(68) were collected.

• Characterization and evaluation: 2,145 accessions were characterized, out of which a total of 1104 accessions comprising finger millet (273), niger (443), castor (288) and winged bean (100) were characterized during kharif 2011 and a total of 1,041 accessions comprising linseed germplasm (835), linseed trial (51), safflower trial (55) and grain amaranth (100) accessions were characterized during rabi 2011-2012.

• Regeneration and multiplication: Regenerated and multiplied 4,266 accessions of germplasm for LTS / MTS. These includes Sesame (1,662), pigeon pea (793), green gram (641) and Abelmoschus spp. (237) during kharif 2011 and 933 accessions comprising chick pea (808) and grass pea (125) during rabi 2011-2012.

• Germplasm Conservation: 330 accessions of germplasm comprising 307 accessions of sesame and 23 accessions of grain amaranth were multiplied and sent for conservation in the National Genebank.

• Germplasm supply: A total of 969 accessions of germplasm of various crops/species were supplied to 22 indenters from different user agencies for their research and improvement programmes within the country.

• Medium Term Storage: A total of 19,849 accessions of germplasm comprising millets and smillets (1,094), pulses (4,844), vegetables (2,120), oilseeds (10,002), wild relatives of crop plants (630), under-utilized crops (1,155) and others (4) are being maintained in the medium term storage module under controlled conditions at 7 0 C .

• Publication- Published four research papers and presented two research papers in the National and International Symposium during the reporting period.

REGIONAL STATION, BHOWALI:

Programme

4.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation and Documentation of Genetic Resources of the Northern Himalayas and Adjoining Plains.

K.S. Negi PGR/PGC-BHO-01.00

Projects

4.1.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of field crops with emphasis on etheno botanical aspects.

K.C. Muneem (upto 30.06.2011), A. K. Trivedi (wef 29.12.2011), K.S. Negi, S. K Verma and P.S. Mehta

PGR/PGC-BHO-01.01

4.1.2 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of temperate horticultural crops.

A.K. Trivedi (upto 28.12.2011), S. K. Verma (wef 29.12.2011), K.C. Muneem (upto 30.06.2011), K.S. Negi and S. Gupta

PGR/PGC-BHO-01.02

4.1.3 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of medicinal and aromatic, wild economically useful, rare and endangered species.

K.S. Negi, K.C. Muneem (upto 30.06.2011), S. K. Verma (wef 29.12.2011), A.K. Trivedi, and A. Raina

PGR/PGC-BHO-01.03


Germplasm augmentation: Three explorations (Two - crop-specific and one survey and identification) was undertaken and 58 (32 + 26) acc. viz. 17 accessions of wheat, 15 accessions of barley, 12 accessions of peach, 14 accessions of pear and 08 accessions of M & AP were recollected from districts of Uttarakashi, Tehri, Rudraprayag, Chamoli; Champawat and Pithoragarh of Uttarakhand .

Germplasm evaluation:A total 993 acc. were evaluated viz. Paddy Rainfed (207), Ricebean (56), Soybean (25), Barley (33), Coriander (152), Fenugreek (62), Lentil (44), Pea germplasm

(Pisum sativum) (50), Pea germplasm (Pisum arvense) (50) Wheat (41), Wheat disease screening nursery (131), Apple (23), Rubus (18), Apricot (14), Peach (08), Pear (12), Plum (06), Chilli (20 ), Allium spp. (wild only-41)

Germplasm Conservation and maintenance: Field Gene Bank (1592): Turmeric (50), Colocasia esculenta (200), Garlic (123), Fruits-338 acc, Ornamentals (109), Allium spp.(41), Arborescent spp. (190), M. & AP. (445), Bamboo (41), Forage grasses (54), Hordeum bulbosum (01).

Germplasm exchange:

• LTS (701) : Rabi (289), Kharif (412) • MTA (166): Wild horse gram (01), Allium spp. (28), Allium spp. Bulbs/rhizomes (05),

Abelmoschus crinitus (01), Strawberry (117), Cucumis sativus ssp. Hardwickii X C. sativus (10), Abelmoschus crinitus, A. callei, A. manihot and A. pungens (04).

New initiatives: Popularization of geranium, lavender and rosemary among local farming communities of Uttarakhand.

• Establishment of mother blocks for bud wood production of citrus and kiwi fruit.

Human Resource Development:

Training imparted (01), Workshops/group meetings/trainings organized during 2011-12 (03), Participation in seminars / symposium / conference / trainings / meetings etc: (19), Lecture delivered (02), Research papers (15), Published Technical bulletins / Technical articles / popular articles (08), Abstract (04).

REGIONAL STATION, CUTTACK:

Programme

3.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation documentation and distribution of genetic resources of Orissa and adjoining regions

D.R. Pani PGR/EXP-BUR-CUT-01.00

Projects

3.1.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of agricultural and horticultural crops in Orissa and adjoining regions

D.R. Pani, R.C. Misra

PGR/EXP- BUR-CUT-01.01

3.1.2 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of medicinal and aromatic plants, wild economically useful and rare and endangered plants of Orissa and adjoining regions

R.C. Misra D. R. Pani

PGR/EXP- BUR-CUT-01.02


Four exploration programmes were conducted and 208 acc comprising cotton (29), Crotalaria (10), M&AP (02), Chili (01), Sesame (01) and trait specific rice germplasm viz. drought tolerant (59), cold tolerant (43) and saline tolerant (63) were collected from Mizoram, Arunachal Pradesh, Sundarban (W.B) and western Odisha.

Ethno-botanical investigation was carried out in tribal rich Similipal forests and 53 species of wild tubers including 18 unreported species as source of supplementary/ famine food plants were identified and documented.

1927 acc comprising cultivated rice (1500), green gram (57), black gram(41), wild relatives of crops (33), M&AP (42) and wild Oryza species (254)were characterized for various morpho-agronomic traits and 1161 acc of cultivated rice were evaluated for BLB (1149) and submergence tolerance(12), out of which, 18 acc were identified as tolerant against BLB and three acc viz. Baliadadha (IC-568957), Champeisali (IC-568918) and Bhuta (IC-568854) were identified as submergence tolerance.

Phyto-chemical screening of fruits of five species such as Diplocyclos palmatus, Mukia maderaspatana, Solena amplexicaulis, Cucumis sativus var. hardwikii and Momordica dioica used as drug plants were validated for their ethno-medicinal properties documented from tribal informants of Odisha.

2686 acc comprising cultivated rice (2318), wild rice (170), sesame (68), Trichosanthes (33), tubers (38), Ocimum spp. (30), Andrographis paniculata (03), Mucuna pruriens (12) and other medicinal plants (14) were multiplied and 2247 acc comprising cultivated rice (2064), cotton (29), Crotalaria (10), M&AP (87), wild relatives (55), chilli (01) and sesame (01) were deposited for LTS.

274 acc comprising Wild Oryza spp (224), greengram (25), blackgram (25) were received and 374 acc comprising Mucuna (11), M&AP (39), niger (36), Trichosanthes (12), taro (63), cultivated rice (115), drought tolerant rice (59), cotton (29) and Crotalaria (10) were supplied for different research programmes.

A total of 1442 acc comprising cultivated rice (494), black gram (23), green gram (21), sesame (54), wild Oryza species (297), M&AP (458), other wild relatives of crops (27), tuber/aroid crops (38), economic plants of agro-forestry importance (24) and horticultural crops (06) are being maintained in field gene bank of the Base Centre.

22 nos. of herbarium specimens were supplied to NHCP, NBPGR, New Delhi. About 250 plant samples of rare/endangered, high valued medicinal plants, fibre crops, wild relatives and economically useful plants of tribal importance were preserved as herbarium specimens.

Publications: Research papers- (Published-04, Communicated-02), Reviewed-01, Abstract-01, Bulletins- 04.

REGIONAL STATION, HYDERABAD:

Programme 1

6.1 Quarantine Processing of Plant Germplasm Under Exchange and Supportive Research

K. S. Varaprasad (upto 31.04.2011) and S. K. Chakrabarty (wef 01.05.2011)

PGR/PQR-BUR-HYD-01.00

Projects

6.1.1 Detection, Identification and Control of Pests Associated With Import and Export of Seed/ Plant Material.

K. Anitha, K.S. Varaprasad (upto 31.04.2011), SK Chakrabarty, B Sarath Babu, N Somasekhar, N. Sivaraj, K Rameash and Babu Abraham


6.1.2 Developing a Database on Pests and Pathogens of Quarantine Significance

B. Sarath Babu, K.S. Varaprasad (upto 31.04.2011) and K Anitha


6.1.3 Quarantine Treatments for Germplasm under Exchange and Developing Detection Techniques and Treatment Schedules for Seed Borne Pathogens

S. K. Chakrabarty and K. Anitha


6.1.4 Post-entry Quarantine Processing of Imported Germplasm

K. S. Varaprasad (upto 31.04.2011), S.K Chakrabarty (wef 01.05.2012), B Sarath Babu, K Anitha, K Rameash and Babu Abraham


Programme 2

6.2 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation,

K. S. Varaprasad (upto 31.04.2011) and S. K. Chakrabarty (wef


Documentation and Distribution of Genetic Resources of South East Coastal Zone

01.05.2012)

Projects

6.2.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Agricultural Crops (Cereals, Millets, Pulses, Oilseeds etc.) and their Wild Relatives.

K Venkateswaran, S R Pandravada, N Sivaraj, N Sunil, and Babu Abraham


6.2.2 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Horticultural Crops (Vegetables, Fruits, Species, Medicinal and Aromatic Plants etc.) and their Wild Relatives.

S R Pandravada, K Venkateswaran, N Sivaraj, N Sunil, and Babu Abraham


6.2.3 Characterization and Evaluation of Wild edible Crops of Leguminosae

N Sivaraj, E R Nayar, S R Pandravada, K Venkateswaran, N Sunil and Babu Abraham


6.2.4 Studies on traits of resistance to thrips and mites in chilli

K. Rameash, B. Sarath Babu, Someswara Rao Pandravada



Programme 1: • Crop germplasm (44, 044 samples) consisting of imports (16, 347) and exports (7,

097 + 20,600) was examined for quarantine clearance. One hundred and twenty-six (96) Phytosanitary certificates were issued to the crop samples meant for export. Quarantine processing of exotic consignments of crop germplasm belonging to Private companies resulted in the resource generation of Rs 16, 66, 203/-.

• Important interceptions on various crops were as follows: Paddy: Aphelenchoides besseyi, Alternaria padwickii and Nigrospora oryzae from Indonesia; Drechslera oryzae from Columbia, A. padwickii, Tillitia barclyana from China; Sunflower: Alternaria helianthi, Botrytis cinerea, Periconia bessoides and Rhizoctonia solani from Argentina, R. solani from Belgium and France; Castor: Alternaria ricini and Fusarium equisetii

from USA; Eucalyptus sp.: Pestalotia sp on from South Africa; Soybean: Peronospora manshurica, intercepted from USA and Canada; R. solani from Canada; Chilli: R. solani from Taiwan; Tomato: Alternaria solani and Pseudomonas syringae pv tomato on from Taiwan; Fingermillet: Alternaria porri and Myrothecium sp. on from Uganda; Maize: Fusarium oxysporum from Indonesia and USA; R. solani from Mexico and Thailand: Drechslera maydis, and R. solani from Kenya, South Africa and Thailand, D. maydis and R. solani from USA

• Fifteen imported samples consisting of soybean samples from USA (3) and Canada (10), and tomato (2) from Taiwan were not found suitable for release due to the presence of downy mildew pathogen, Peronospora manshurica and Pseudomonas syringae pv tomato, respectively, hence rejected. PEQ inspections were conducted on crop germplasm (2352 samples) (Private companies- 1134 and ICRISAT- 1162). Of the total samples inspected, 56 transgenic events of rice, and corn, imported by the Private industry were included.

• Drechslera oryzae, D. carbonum on paddy from Philippines; shoot borer damage and leaf blight due to Drechslera species on maize, zonate leaf spot (Gloeocercospora sorghi) on sorghum from France, Alternaria spp., Bipolaris sacchari, Curvularia lunata and Periconia byssoides on sorghum from South Africa; Alternaria helianthi, A. zinniae, Periconia bessoides, and Cercospora sp on sunflower hybrid from Australia were recorded. Incidence of Tobacco mosaic virus, blank shank and aphid infestation noticed on tobacco from USA.

• During the reporting period, import germplasm (14, 043samples) was subjected to quarantine mandatory treatments before release.

• Pigeonpea seeds infected with Phoma medicaginis were tested to study the seedborne nature of the pathogen using standard blotter test. Infection was observed even in the surface sterilized seed indicating the internal seedborne nature of the pathogen.

• Benlate+thiram (1:1) treatment was effective in reducing seedborne infection and increasing the germination percentage. Among the treatments, thiram 3 g a.i/kg was least effective as it could record high infection of 60.0%

• During the year under report information on the eight medicinal plants viz., Trigonellafoenum-graecum, Terminaliatomentosa, Crateavareligiosa, Gingiberofficinale, Petroselinumcrispum, Trifolium pretense, Zingiber mioga. Lepidium meyenii has been compiled

• Publication: Three Programme 2:

• Seven explorations were undertaken one each for the collection of cold tolerant rices from Tamil Nadu and Kerala (NICRA Project); small millets, buckwheat and French bean from Arunachal Pradesh (special mission in NEH region) Onion (2 surveys), Palmyrah palm, Zehneria angulata and Spine gourd and other agri-horticultural diversity from tribal tracts of Visakhapatnam, Andhra Pradesh. A total of 540 accessions were

collected in these seven surveys. In addition, a total of 28 accessions of wild legume germplasm (Orissa-4; Andhra Pradesh-24) were augmented.

• A total of 1, 972 accs. of germplasm of horticultural crops comprising of sorghum, small

millets linseed, horsegram, green gram brinjal, chilli, cowpea , dolichos bean, field bean, french bean, tomato, yard long bean and wild legumes were characterized / evaluated / multiplied. Field screening of 186 chilli germplasm was carried out in ABD against thrips and mites. Bio-chemical analysis was carried out to quantify total phenols in the selected accessions of chillies. Promising accessions were identified for different economically important traits. A Pulses germplasm field day was organized at NBPGR,RS,Hyderabad on 25.8.2011 wherein six participants from various research stations of Acharya N.G.Ranga Agricultural University participated and made trait based selections.

• A total of 316 accessions comprising of small millets (barnyard millet, kodo millet, finger millet, proso millet, Italian millet) buckwheat, greengram, chilli, tomato and wild legume germplasm were sent to NGB for long-term conservation. Also facilitated the long-term storage conservation in the NGB of 12,712 voucher samples of the material received from ICRISAT and processed for the Svalbard Global Seed Vault, Norway

• Accomplished Germplasm Registration of a Dolichos bean accession (INGR-11031/ IC-383192) field resistant to Anthracnose and Aphids.

REGIONAL STATION, JODHPUR:

Programme

5.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources in Arid and Semi-Arid Regions

N.K. Dwivedi (upto 08.02.2012) and Om Vir Singh (wef 09.02.2012)

PGR/GEV-BUR-JOD-01.00

Projects

5.1.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Cereals, Pearl Millet, Minor Millets and Horticultural Crops.

A K Singh, N. K. Dwivedi (upto 08.02.2012) and Om Vir Singh (wef 09.02.2012)


5.1.2 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Legumes and Oilseeds.

A K Singh, N. K. Dwivedi (upto 08.02.2012) and Om Vir Singh (wef 09.02.2012)


5.1.3 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Fodder, Forage, Fuel, Medicinal and aromatic and other Economic Plants.

N.K. Dwivedi (upto 08.02.2012), Om Vir Singh (wef 09.02.2012) and A K Singh



• Augmented germplasm by adding 156 accessions of various crops species. • The 2645 accessions of pearl millets and arid legumes evaluated and identified high

yielding genotypes of various crops. • Seed of the 1650 accessions of arid legumes and oil seed crops multiplied. • The 33,856 accessions of different crop species were maintained in MTS. • The 750 accessions of horticultural and economic plants are being maintained in the field. • A total of 458 accessions of various crop species were supplied to the indenters. • Germplasm field day was organized on Oct., 12, 2011.

BASE CENTRE, RANCHI:

Programme

7.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation, documentation and distribution of genetic resources in Bihar, Jharkhand and adjoining areas

J.B. Tomar

PGR/PGC-BUR-RAN-01.00

Projects

7.1.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of agriculture crops, their wild relatives and economic

J.B. Tomar, Santosh Kumar and A.K.Gupta


species including medicinal plants

7.1.2 Augmentation, characterization, evaluation, maintenance, regeneration, conservation, documentation of genetic resources of horticultural crops and perennial medicine

J.B. Tomar, Santosh Kumar and A.K.Gupta



• 113 samples of different plant species were collected from three explorations in the areas of Dumka, Jamtara, Pakur and Deoghar districts of Jharkhand. The overall collections made during three expedition were-s Abelmoscus crinitus Jungali bhindi (2), Abelmoscus sp./Jungali bhindi (3), Abrus precatorius/Ratti (1), Acasia katachu/Kattha (1), Acorus calamus/ Ghodabach (6), Aloe vera Ghritkumari (1), Alpinia galangal/Mahavir bach (1), Amorphophallus sp./Wild oal (1), Andrographis paniculata/ Kalmegh (11), Aristolochia indica/ Isharmool (1), Asparagus racemosus/ Shatawar (15), Bacopa monierii/ Brahmi saag (1), Bambusa sp./Bans (1), Barleria sp./Toot ghas (1), Boswelia serreta/Salai (3), Buetteneria herbacea /Kamraj (1) ,Chlorophytum arundinaceum/ Safed musali (5), Clerodendron serratum /Ghato(1), Costus speciosus/Kevakand (1), Curcuma amada/ Vayadi (1),Curcuma angustifolia/Tikhur (1), Hedychium spicatum Kapoor kachari (1), Ipoemia sp./Pelwa saag (1), Lassia hetrophyla/ Kantasaru(1), Mallotus phillipinensis/ Kamala(1), Ocimum gratissimum/ Bhim Tulsi (1), Ocimum gratissmum/ Bantulsi(2), Ocimum sp./Tulsi(1), Pandanus sp/.Kebda(1), Pentapetes phoenisia /Dopharia (2), Psoralea corylifolia Babchi (1), Rauvolfia serpantina/ Sarpgandha (3), Semecarpus anacardium/ Bhelwa (1), Smilex sp./Ramdatun(sitia) (1), Soymida fabrifuga Rakt rohan (2),Sterculia sp./(1), Symplocos racemosa /Lodh (3) , Tinospera cordifoium Ghuruch (9),Trichosanthes sp. /Bankundari (1), Typhonium sp./Ban oal (1), Typhonium trilobatum/Nirvish (1) Urginia indica/Banpyaj (4),Vitex pedencularis/ Charai godba(1), Zingiber cassumunar/ Jungali adarak (1), Zingiber zerumbet/ Jungali adarak (1), Aromatic plant (1), Chidia kanda (1), Bhimraj Tulsi(1), Chidia kanda1Bhui chaw (1) Ban padum (1), Streblus asper /Sohra (1), Bhimraj(1), Mardraj (1), Raini (1), Tueipinach(1), Bhimraj (1)

• 362 accessions of Kulthi germplasm were sown on 12.07.2011 for evaluation in augmented design. Two checks Madhu and Birsa Kulthi-1 (BK1) were used in the experiment. The observation on primary branches per plants, days to 50% flowering, pods/plant, pod length (cm), plant height (cm), no. of seeds/pod, yield/plant (g), 100 seed weight (g), growth habit, growth pattern, leaf color, leaf surface, pod shape, pod surface and seed color were recorded

• A total of 39 accessions of Mucuna germplasm were sown on 05.07.2011 for evaluation in RBD with two replications. The observations were recorded on plant height, leaflet shape, odd leaflet length, odd leaflet width, days to flower initiation, inflorescence length, no. of inflorescence per plant, flower per inflorescence, flower color, no. of pods per cluster, no. of pod cluster per plant, pod pubescence intensity, pod pubescence color, pod shape, pod length, pod width.

• 11 accessions of Cajanus cajan were sown on 04.07.2011 in two replications. The number of rows per accession was kept 5 with a spacing of 50×10 cm. Row length was kept 7 m and spacing between accessions was kept at 1 m. Two checks one national (NDA-1) and other local (Bahar).

• Germplasm maintenance in field gene bank- Jackfruit (154), Tamarind (51), Jamun (46), Bael (53), Barhal (14), Aonla (19) Mangifera (19) Musa sp. (34) Lawsonia indica (25) Moringa oleifera (14) Medicinal & Aromatic Plants (240 species)

• The material sent in cryobank for long term conservation are as Costus speciosus (8),

Guizotia abyssinica (5), Atylosia scarabaeoides (1), Sida acutifolia(1), Spilanthes paniculata(1), Abelmoschus moschata(1), Cardiospermum helicacabum(1), Celestrus paniculatus(1)

REGIONAL STATION, SHILLONG:

Programme

9.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation, documentation and distribution of plant genetic resources in north-eastern India

S.K. Verma (upto 12.12.2011) and A. K. Mishra (wef 13.12.2011)

PGR/PGC-BUR-SHL-01.00

Projects

9.1.1 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of agricultural crops (paddy-low land/upland, maize, and mustard) and their wild relatives

A.K. Misra, S.K. Verma (upto 24.12.2011), Ranbir Singh, and Somnath Roy


9.1.2 Augmentation, characterization, evaluation, maintenance, regeneration, conservation and documentation of genetic resources of horticultural crops (chilli, ginger, turmeric, yams, taros, Citrus, banana and passion fruit) and their wild relatives

S. K. Verma (upto 24.12.2011), A. K. Misra (wef 25.12.2011), Ranbir Singh, and Somnath Roy


9.1.3 Augmentation, characterization, evaluation, maintenance,

Ranbir Singh, S. K. Verma (upto 24.12.2011),

PGR/PGC-BUR-SHL-

regeneration, conservation and documentation of genetic resources of under-utilized (UU) and and their wild relatives

A. K. Misra, and Somnath Roy

01.03


• Three explorations were conducted in Manipur, Mizoram and Sikkim. The station was also collaborated in two other explorations to Arunachal Pradesh under NEH exploration plan.

• A total of 41 aromatic rice (chakhao rice) germplasm were collected from Manipur. Collection of multi-crop germplasm comprising vegetables (14), rhizomatous crops (6), fruits (4), cereals (4) and 10 other crops were made from Mamit, Aizawl and Champhai districts of Mizoram. From the exploration trip in the East, West and South districts of Sikkim maize (46), rice (17), buckwheat (22) and Vigna spp. (37) were collected.

• A total of 987 accessions of different agri-horticultural crops comprising maize (151), upland paddy (273), lowland paddy (229), rice bean (155), Coix (54), Perilla (40) and buckwheat (85) were characterized for agro-morphological traits. Regeneration of chilli (95) were also undertaken.

• A unique maize line (MCM-11/01) with multiple cobs (3-5) was selected from trial plot of an indigenous collection (IC524594).

• Lowland paddy germplasm were evaluated for leaf blast incidence and considerable variation was observed for percent disease incidence (4.4-77.8%).

• In field gene bank, 610 accessions of various horticultural crops and M&APs were maintained.

A total of 434 germplasm of lowland paddy (157), upland paddy (125), maize (95), Perilla (34) and Coix (25) were sent to NGB for long term conservation.

REGIONAL STATION, SHIMLA:

Programme

10.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources in North-Western Indian Himalayan Region

V.D. Verma (upto 04.07.2011) and J. C. Rana (wef 05.07.2011)

PGR/GEV-BUR-SHM-01.00

Projects

10.1.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Pseudocereals and Pulses and other Lesser Known Hill Crops.

J.C. Rana and V.D. Verma (upto 26.02.2012)


10.1.2 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources of Temperate Fruits, Vegetables, and Medicinal & Aromatic Plants.

V.D. Verma (upto 26.02.2012), J.C. Rana (wef 27.02.2012), and Sandhya Gupta



• Three explorations were conducted and diversity collected represent maize (51), kidney bean (27), rice (14), wheat (17), buckwheat (25), barley (11), mustard (15), amaranth (32) and others (26).

• Significant collections include landraces of red rice, traditional varieties of maize, kidney bean and grain amaranth.

• A total of 2013 germplasm accessions were grown for characterization, evaluation and multiplication. Genetic variability for seed and pod colour, shape and size was recorded in pea germplasm and also for other traits in different crops.

• Germplasm accessions viz. EC598556, EC598558, EC5985711, EC598573, EC598731 showed superiority for multiple traits like for pod length, pods/plant, seeds/pod, seed weight. In rice bean IC26973, IC18465 and IC18183 were found promising for number of pods and seed yield/plants.

• A total of 64 accessions of field pea were screened against 4 isolates viz. Rangway, Trilokinath, Stingri, Kangra of powdery mildews (Erysiphe pisi) prevalent in Western Himalaya under laboratory conditions. Accessions IC208366, IC208378, IC218988, IC267142, IC278261, IC381866, and EC598814 showed resistance against all the four isolates.

• Among fruits, 202 accessions of apple (41), pear (24), plum (41), apricot (22), peach (33), and walnut (41) were characterized and evaluated.

• Wide range of variability was recorded for traits like fruit colour, shape and size. In

peach EC038736, EC312408, EC552643, EC552644 and EC387511were found superior for multiple traits while ‘Silver King’ of nectarine was found promising for fruit size and colour.

• A total of 330 accessions were supplied for Long Term Storage while 10377 accessions of seed crops and 1185 of perennial crops were conserved in the MTS and FGB, respectively.

• Germplasm comprising 2215 seed samples of agricultural crops and 815 rooted plants and 967 bud sticks of fruit crops were supplied to researchers across the country.

• Eight research papers in per reviewed journals, one book chapter and one book was published.

• One brainstorming session and two PGR biodiversity exhibitions were organized.

REGIONAL STATION, SRINAGAR:

Project

8.1 Augmentation, Characterization, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Genetic Resources from J&K

Om Vir Singh (upto 07.02.2012) and S M Sultan (wef 08.02.2012)

PGR/PGC-BUR-SRI-01.00


1. Germplasm Characterization and Evaluation: A total of 240 accessions of wheat,114 accessions of barley and 124 accessions of mustard were evaluated during 2010-2011.Some promising genotypes in each of these crops were identified.

A total of 335 accessions of wheat, 280 accessions of barley and 108 accessions of mustard were sown during rabi 2011 and are currently being characterized and evaluated for their morphological traits under rainfed conditions of Himalayas.

2. Germplasm Exploration and Collection: 16 accessions of chillies were collected from different

areas of Kashmir valley falling in the Districts of Srinagar,Budgam and Pulwama in collaboration with CITH and deposited at the HQ.44 accessions of walnut,50 accessions of almond and 12 accessions of garlic were also collected from different villages of Kashmir valley and deposited at the HQ along with the passport data.

3. Maintenance of Germplasm:35 accessions comprising of Dioscorea deltoidea , strawberry, Allium cepa var.proliferatum (pran), mint and Iris spp. are maintained in the field. Recently planted five accessions each of wild and endangered fruit trees of quince,ambri and maharaji apple,cherry,bird cherry,bitter almonds,wild apricots,Celtis,peaches and plums are also maintained.

4. Publications:

Om Vir and Sheikh M. Sultan (2011). Identification of genotypes of wheat (Triticum aestivum L.) for specific adaptation using qualitative and quantitative genotype x environment interaction and regression analysis in the rainfed condition of Himalayas. Indian J. of Agricultural Research ( Accepted vide No.AR 3731).

Om Vir and Sheikh, M. Sultan ( 2011). Estimation of genetic parameters and characters associations in Indian Mustard (Brassica juncea). Indian J. of Agricultural Research (Accepted vide No. AR 3823).

REGIONAL STATION, THRISSUR:

Programme

11.1 Augmentation, Characterisation, Evaluation, Maintenance, Regeneration, Conservation, Documentation and Distribution of Plant Genetic Resources in Southern India including Goa and Andaman & Nicobar Islands (includes 5 Projects)

Z. Abraham (upto 12.02.2012) and N. K. Dwivedi (wef 13.02.2012)

PGR/GEV-BUR-THR-01.00

Projects

11.1.1 Augmentation, characterisation, evaluation, maintenance, regeneration, conservation, documentation and distribution of genetic resources of field crops and their wild relatives

M. Latha, Z Abraham, N. K. Dwivedi (wef 13.02.2012), K.I. Asha and S. Mani

PGR/GEV- BUR-THR-01.01

11.1.2 Augmentation, characterisation, evaluation, maintenance, regeneration, conservation, documentation and distribution of genetic resources of tuber and fruit crops and their wild relatives

K.I. Asha, K. Joseph John, R Asokan Nair and S Mani

PGR/GEV-BUR-THR-01.02

11.1.3 Augmentation, characterisation, evaluation, maintenance,

Z Abraham, N. K. Dwivedi (wef 13.02.2012),

PGR/GEV- BUR-THR-

regeneration, conservation, documentation and distribution of genetic resources of spices, their wild relatives and medicinal & aromatic plants

K. Joseph John, M. Latha, and S. Mani

01.03

11.1.4 Augmentation, characterisation, evaluation, maintenance, regeneration, conservation, documentation and distribution of genetic resources of tropical vegetables and their wild relatives

K Joseph John, M. Latha and R. Asokan Nair


11.1.5 Use of in vitro technology for mass propagation and conservation of clonally/ vegetatively propagated crops and their wild relatives

Z. Abraham (upto 12.02.2012), N. K. Dwivedi (wef 13.02.2012), K.I. Asha and A. Indira Devi



• Four exploration and collection missions were conducted for the collection of vegetables from Goa, cucurbits from Mizoram and Tripura and trait specific deep water rice from Assam. In all 372 accessions comprising of aboli (3), Alpinia sp.(2), ash gourd (23), Barleria longiflora (1), betel leaf (1), bird's eye chilli (1), bitter gourd (13), black gram (1), bottle gourd (10), chillies (4), Chinese lard fruit (1), Chinese spinach (11), cocks comb (1), cocoyam (1), cucumber (30), taro (1), drumstick (5), elephant foot yam (1), greater yam (4), horse gram (1), jasmine (3), Kedrostis wild (1), kokum (1), lesser yam (1), marigold (2), melon (4), okra (3), pointed gourd (1), potato yam (1), pumpkin (28), rice –aromatic (2), rice-deep-water (59), rice bean (2), ridge gourd (9), satkutia (1), small bitter gourd (4), smooth gourd (4), snake gourd (10), snap melon (4), spleen amaranth (2), sponge gourd (11), sweet gourd (3), sweet potato (4), sweet-flag (1), teasel gourd (71), water melon (2), wild bitter gourd (5), wild cucumber (7), wild Trichosanthes (8) and yard long bean (3) were collected.

• During rabi 2010-11, 563 accessions (lowland rice:170; horsegram:335; small bittergourd:3; Solanum insanum:05; Cucurbita species:06; cushaw: 05; and pumpkin:22; kacholam:17) and during kharif 2011-12, 180 accessions (lowland rice:85; upland rice landraces:34; Malabar tamarind:36 and kokum:25) were characterized/ evaluated.

• 10419 germplasm accessions are maintained in field genebank (2498) and in MTS facility (7921)

• 110 multiplied accessions comprising 60 accessions of forage crops (Alloteropsis cimicina-2, Apluda mutica-1, Bothriochloa pertusa – 1, Cenchrus biflorus-3, Cenchrus setigerus-9, Chloris barbata-1, Chloris Guyana-8, Chrysopogon fulvus-1, Dichanthium annulatum-12, Eragrostis tenella-1, Eragrostis tremula-1, Eriochloa procera-1, Heteropogon contortus-21), 3 of Momordica cochinchinensis, 15 of pumpkin,1 of

Cushaw 1 of Vigna umbellata, 15 of V. dalzelliana, 6 each of V. radiata var. sublobata and V. trinervia var. bourneae and 62 of collected samples comprising of deep water rice (59), aromatic rice (2) and chilli (01), and 1 of tomato received from KAU were deposited for LTS at NGB, NBPGR, New Delhi.

• 305 accessions comprising of forage species (248), rice (42), Oryza species (8), amaranth (1), pearl millet (4), Panicum species (2) were received NBPGR, New Delhi and 5 accessions (Aloe vera-2, Caralluma edulis-1, Moringa concanensis-1, M. oleifera-1) were received from NBPGR RS, Jodhpur.

• 78 accessions comprising of Dioscorea alata (35),D. esculenta (7) and wild Dioscorea (36) and 18 of poly-embryonic mango were transferred to CTCRI, Thiruvananthapuram and CISH, Lucknow respectively for maintenance in field genebank as NAGS and utilisation.

• 163 accessions of various crops/ species were supplied to 9 user agencies under MTA. While 100 accessions of horsegram were sent to 2 different centres of NNRP on Arid Legumes for multi-location evaluation trial. Thirty-two accessions in 9 species of Abelmoschus were supplied to NBPGR, New Delhi. Fifty-seven accessions comprising Alpinia calcarata (16), A. galanga (12) and Malabar tamarind (29) were sent to GED, NBPGR, New Delhi for chemical evaluation.

• Tissue culture work was continued in brahmi (Bacopa monnieri), cassava (Manihot esculenta), ginger (Zingiber officinale), lesser galangal (Alpinia calcarata), and wild species of Dioscorea and Curcuma. In total 55 accessions in these 5 crops and 10 wild related species were maintained.

• Two Tribal Agro-Bio-diversity Fairs were conducted. First at Attapady, Palakkad district on 29/03/2012 concentrating on Muthuva, Irula and Kurumba tribes and second at Kannavam forest, Kannur district on 31/03/2012 concentrating on Agro-biodiversity of Kurichaya tribe.

• 222 students along with their faculty from various state Agricultural Universities and other traditional universities were enlightened with PGR activities of the station.

• Dr. NK Dwivedi, Principal Scientist has joined the station as Officer in charge on 13/02/2012.

• Revenue generated: Rs.43,367/-

• New Projects: Two new projects namely ‘Geo-informatics for assessment of diversity distribution of agri-horticultural crops’ (PI: DP Semwal; Co-PI: DC Bhandari, KC Bhatt; Associate: Rakesh Singh) and ‘Molecular based detection, identification and characterization of fungi and bacteria infecting plant genetic resources in quarantine’ (PI: A Kandan; Co-PI: J Akhtar, Anirban Roy; Associate: Dinesh Chand) were presented in the meeting. It has been decided that the above mentioned projects may be included in the revised institute projects likely to be operational from April 2013.

• Dr. Celia Chalam has been nominated as the next IRC Member Secretary.

New Recommendations During the scheduled presentations and subsequent discussions, the following recommendations were emerged. These have been grouped under General recommendations and specific recommendations for respective Division/ Units/ Regional Stations. Action on these recommendations should be taken and reported by concerned HoDs/ Officer- in- Charge, Unit/Regional Stations, unless some specific action is required from individual Scientists as indicated.

General Recommendations 01/12 As NBPGR has been identified for two platforms, viz., Agro-biodiversity and Genomics

in XII Plan, activities such as regeneration, maintenance, multiplication and supply of material, etc. to be integrated with the activities of the Platform on Agro-biodiversity.

(Action: All Members) 02/12 Since, there are less number of crop scientists in Exploration Division, it was decided

that crop experts from other Divisions be included in the Exploration programme, whenever required.

(Action: All Members) 03/12 All the germplasm to be supplied from genebank should be given mandatory pesticidal

treatment by PQD before supplying to the indenter for multiplication/ regeneration, etc. (Action: All Members)

04/12 For registration of germplasm with biochemical parameters, the tested sample should be either from two different seasons/years from single location or harvest from two locations at one season in a given year.

(Action: All Members) 05/12 Scientists from different Divisions/Units/ Regional Stations were encouraged to bring

out quality publications in journal of international repute. (Action: All Members)

06/12 Since NBPGR has initiated research programme in frontier areas, the HRD requirement of the Bureau’s scientists in each Division/Unit/Regional Station should be prepared for effective utilization of the allotted fund under HRD in XII Plan.

(Action: All Members) 07/12 To fill up the vacant posts of scientists/ technical staff, proposal need to be sent on

priority as ICAR has agreed to fill up the vacant positions. (Action: All Members)

08/12 The following scientists will give seminar on the following topic: (Action: All Concerned)

Dr DB Parakh to deliver a seminar on ‘Role of Cryopreservation in eliminating viruses’. Dr Rameash Senior Scientist, NBPGR Regional Station Hyderabad to deliver a seminar on ‘Nanotechnology’ at Head Quarter. Dr. KV Bhat to deliver a seminar on ‘DNA fingerprinting’. Dr N.K. Gautam to deliver a seminar on ‘Best procedure to be followed for germplasm evaluation keeping in view the international standards being followed at ICRISAT or ICARDA’. Dr Hanuman Lal to deliver a seminar on ‘Advanced statistical techniques used for characterization and evaluation of germplasm’.

09/12 Subject matter experts will be called from time to time to deliver lectures on emerging issues related to PGR management.

(Action: OIC, PME Cell) Plant Exploration and Germplasm Collection Division 10/12 Gap analysis (crop-wise and region-wise) of landraces, on the basis of NGB database,

should be done before planning future explorations. To accomplish the task, Exploration Division may seek information on crop-wise NGB holdings from Conservation Division.

(Action: Head, Expl. Div.) 11/12 Information on wild relatives and landraces of major cultivated crops to be compiled and

updated. (Action: Head, Expl. Div.)

12/12 Ethno-botanical and passport information on Medicinal & Aromatic Plants (M&AP) after verification may be made available along with photographs for AICRP Workshops/ Group Meetings.

(Action: Head, Expl. Div.) 13/12 A team of scientists including outside expert to be constituted for taxonomical

identification/ confirmation of species in case of ambiguity. In this regard, requirement of tools and capacity building etc. to be provided.

(Action: Head, Expl. Div.) 14/12 The collection of Allium species germplasm from Arunachal Pradesh and Sikkim to be

taken up on priority. (Action: Head, Expl. Div.)

Germplasm Exchange Unit 15/12 There is poor response on feedback on supplied germplasm to the indenters and as a

result their potential for utilization in crop improvement programs remain obscured. It has also been observed that the indenters are reluctant to send seeds of exotic accessions for conservation in NGB after regeneration/multiplication. Keeping these in view, a Committee may be constituted under the Chairmanship of Dr. Arjun Lal to frame stringent guidelines to address the above issues.

(Action: OIC, GEx.) Plant Quarantine Division 16/12 Possible collaboration with National Bureau of Agriculturally Important Insects (NBAII)

and National Bureau of Agriculturally Important Microbes (NBAIM) should be explored for identification of insect pests and pathogens, wherever required.

(Action: Head, PQD) 17/12 The information available with other Gene Banks regarding pest-free conservation (PFC)

of germplasm using different techniques should be compiled for our use, if any from the available literature.

(Action: Head, PQD) AKMU 18/12 The data entered in the database may be checked and corrections, if any may be

communicated for making necessary changes. (Action: OIC, AKMU

19/12 The discrepancies between accession numbers vs. crop name mentioned in Evaluation and Conservation databases need to be resolved for harmonization.

(Action: OIC, AKMU)

PGR Policy 20/12 The contribution of NBPGR in PPVFRA registered varieties may be compiled to know

the extent of germplasm utilization. (Action: OIC, PGRPU

Germplasm Evaluation Division 21/12 Since ICAR has directed to use the SAS (Statistical Analysis Software) for statistical

analysis, it was suggested that Dr. Hanuman Lal to use this software for analysis of PGR data and provide training to the scientist.

(Action: Head, GED) 22/12 Catalogues on ‘Minimal Descriptors of Millets’ should be published so as to facilitate

inclusion of information on millets in Evaluation Database. (Action: Head, GED)

23/12 Few (8-10) hardbound copies of characterization and evaluation report on Kharif and Rabi crops to be prepared for keeping records as well as for reference.

(Action: Head, GED) 24/12 The research projects entitled ‘Standardization of agro-techniques of elite medicinal

plants (Giloe and Shatavar)’ and ‘Development of Core set in Brinjal (Solanum melongena L.)’ are proposed for closure.

(Action: Head, GED) Germplasm Conservation Division 25/12 GCD will provide crop-wise information on NGB holdings to Exploration Division to

facilitate gap analysis and plan further explorations accordingly. (Action: Head, GCD)

26/12 IC numbers to the released varieties should be given only after conducting the seed health testing/germination test to avoid any discrepancy arising out of rejection of material.

(Action: Head, GCD) 27/12 Since large number of chickpea germplasm (~19,000) will be received after evaluation,

priority should be given to process it for long-term conservation to avoid the deterioration due to high humidity. The seed health testing of the same would be carried out in phase manner subsequently.

(Action: Head, GCD) 28/12 Priority should be given for regeneration of voucher samples kept at MTS.

(Action: Head, GCD) 29/12 Dr Sherry Rachel Jacob, Scientist, will be Co-PI in the research project entitled

‘Conservation of Grain Legume Germplasm using Conventional Seed Storage Methods’. (Action: Head, GCD)

30/12 The research projects entitled ‘Investigating Seed Dormancy, Seed Storage Behaviour, Physiological and Biochemical Changes during Storage’ is proposed for closure.

(Action: Head, GCD) TCCU 31/12 Status of in vitro germplasm sent to CTCRI may be assessed and accordingly, necessary

action on shifting of cultures to CTCRI may be taken. (Action: OIC, TCCU)

32/12 Since, the in vitro culture of Bacopa monneri germplasm sent from TCCU has been successfully established at Regional Station Thrissur; the maintenance of the same at HQ to be discontinued.

(Action: OIC, TCCU) 33/12 Dr. Anju Jain, Senior Scientist, will be Co-PI in the project entitled ‘Studies on Genetic

Stability of In Vitro Conserved and Cryo-preserved Germplasm’. (Action: OIC, TCCU)

NRC DNAFP 34/12 Efforts may be made to get the DNAFP and GM detection laboratories upgraded with the

state of the art technology. (Action: OIC, NRCDNAFP)

Regional Stations NBPGR RS, Akola: Nil NBPGR RS, Bhowali: Nil NBPGR RS Cuttack: Nil NBPGR RS Hyderabad 35/12 Data on number of accessions (Indian origin) exported to Seed Vault to be reported.

(Action: OIC, Reg. Stn. Hyderabad.) 36/12 The fees being charged for seed health testing of samples of private companies to be

revised with the approval of competent authority. (Action: OIC, Reg. Stn. Hyderabad.)

37/12 Efforts should be made to get the complete core collection of mandated crops of ICRISAT.

(Action: OIC, Reg. Stn. Hyderabad.) 38/12 The research projects entitled ‘Developing a Database on Pests and Pathogens of

Quarantine Significance’ and ‘Quarantine Treatments for Germplasm under Exchange and Developing Detection Techniques and Treatment Schedules for Seed Borne Pathogens’ are proposed for closure.

(Action: OIC, Reg. Stn. Hyderabad.) NBPGR RS Jodhpur: Nil NBPGR RS Ranchi: Nil NBPGR RS Shillong: Nil NBPGR RS Shimla: Nil NBPGR RS Srinagar: Nil NBPGR RS Thrissur: Nil

In the concluding remarks, the Chairman expressed overall satisfaction on the progress of the research work and emphasized for better coordination for smooth functioning of the Platforms.

The meeting ended with a vote of thanks to the Chair.

Sd/- Dr K K Gangopadhyay (Approved By Dr K. C. Bansal, Chairman, IRC)

Annexure-I

Table: List of new scientists joined in NBPGR during 2011-12

Sl. No. Name Discipline Date of Joining

1. Dr A Suma Economic Botany 23.12.2011 2. Ms Sheel Plant Biotechnology 24.12.2011 3. Dr Monika Singh Plant Biotechnology 02.05.2012

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