natamycin a oelofse

8/3/2019 Natamycin a Oelofse

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NATAMYCIN

- whats the story -

Adriaan Oelofse

SASEV/SAWWV Na-Oes Betragting 7 Mei 2010

WINE CELLAR SERVICES (PTY) LTD.


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TCA Cork Taint

Brett

Burnt Rubber Aroma

Smoke Taint

TBA Taint

NATAMYCIN

Latest Buzzword

STORY: What do you know about Natamycin?

Where can I test it?

Apparently in grape concentrate coming from Argentina?


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ORIGIN: (DSM Fact sheet)

Antimicrobial substance (antimycotic)

bacterium Streptomyces natalensis

- soil micro-organism isolated in Natal

naturally occurring and prevents growth of yeasts and moulds

Bacteria are not affected by natamycin

no colour, odour or taste

white to yellow crystalline powder

What is Natamycin?


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APPLICATIONS:

Food: FDA approved in 1994

Widely used as preservative

- cheeses (whole, shredded, soft cheeses),

- Other dairy products (yogurt)

- sausages

Some countries - juices, wine

Medical: antifungal cream, in eyedrops, lozenges

very effective in extremely small quantities

prevents growth of most food spoilage moulds and yeasts

Natamycin is applied in liquid sprays and dips or as powder

Where is it used?


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USE

As antifungal agent (grape concentrate and wine) To prevent re-fermentation in the bottle (semi-sweet wine)

To stop alcoholic fermentation

Microbial stabilization after bottling Control Brettanomycesyeast spoilage

LEGISLATION

LIQUOR PRODUCTS ACT, ACT 60 OF 1989

Strictly prohibited to export bottled wine containing Natamycin to the EU

USA,Argentina, Chile, Canada, Australia and New Zealand

use of Natamycin in certain countries is legal South Africa

IMPACT (potential)

40 -50% of SA wine production exported to EU

Brand damage & SA Wine Industry damage on international level

During winemaking ?


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Wine problem?

Joint FAO/WHO Expert Committee

recommended quantities of Natamycin

pose absolutely no threat to food safety or human health

not toxic

Recognition:

EU has E number E235 (food additive)

US - natamycin

Where is the problem?

not part of approved list of additives by OIV

Can now test for it!

Impact (Actual) Millions R spent on analysis

South African wine under spotlight & Rejection costs

Alternatives can be costly


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In bottle

Natamycin

PimaricinNatamax

Delvocid

Actistab

Sources of Natamycin

microbes

winemakingpractices

additives

vineyard

corks

grape concentrate

microbes

winemakingpractices

additives

vineyard

corks

grape concentrate
http://www.flickr.com/photos/minebilder/86433438/


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Wineries implement strict QC protocols Alternatives

Export Certificates (since 15 Jan 2010) NDA issue - export certificate and VI1 document Natamycin test compulsory for export

Request from suppliers Declarations (Products free of Natamycin)

COAs (Certificate of Analysis) Analysis of Natamycin

CAF Central Analytical Facility Stellenbosch University NDA Department of Agriculture, Division liquor products PAREXEL Bioanalytical lab, Mosselbay

Rejected wines (Natamycin > 5 ug/L) Anything positive retested by another lab & instrument, then rejected Sold locally & other markets Treatments investigated

Lab request (CAF) Proper paperwork

Developments


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Contamination sources PRIMARY GRAPE CONCENTRATE, AS DIRECT ADDITIVE SECONDARY wine tanks, during bottling & equipment, paper towels,

carton boxes, un-used corks, water Clean: Water, Oak chips, most latest corks

Lab analysis More than 5612, 187 positive, 4373 dry wines, Unsure of screwcap numbers, most natural corked and synthetic

International wines tested 12 German wines, all negative Need more

WINETECH Project funded (Dept Chemistry & CAF)

Focus: Improved testing method & Breakdown products

Illegal in EU, Korea, Japan, Chile, USA, AUS, NZ, Canada,Argentina, Georgia

Important info


10/13

ITS ABOUT MICROBIAL STABILITY!

Potassium sorbate

DMDC Velcorin

Sterile filtration/ Cross flow

Centrifugation Pasteurization

PEF (Pulsed electric fields)

Alternatives


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GENERAL

Potassium sorbate is the potassium salt of sorbic acid. Food preservative (E number 202).

effective in a variety of applications: food, wine and personal care.

IN WINE

Selective anti-fungal, anti-microbial properties inhibits yeast very effectively not effective against many bacteria

Not added during MLF Active LAB metabolise and form Geranium-like smell (irreversible)

MUST use with SO2 to control acetic acid and malolactic bacteria Very stable in wine.

Legal limit: not exceed 200 mg/L

Potassium Sorbate


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IN WINE

Dimethyl dicarbonate (DMDC)

approved by FDA, WHO, and EU (Food preservative E number 242)

broad spectrum of effectiveness against spoilage microorganisms

allowed in South Africa, Australia, New Zealand, EU, USA, others

Bottling process technology

to prevent re-fermentation in wines effective against undesirable yeast like Brettanomyces

to decrease the amount of SO2 used

Breaks down completely into methanol and CO2 in hours

Dosages 75 -200 mg/L depending on sugar concentration

Added to wines >5g/l RS

To help stabilize unfiltered premium wines:

No influence on the sensory properties of the wine

Velcorin DMDC


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THANK YOU

Central Analytical Facility (US) Tel: 021 808 5825

Dept Agriculture: Div Liquor Products Tel: 021 809 0224

THALES WINE CELLAR SERVICES Tel: 021 887 1235

Email: [email protected]

Cellar hygiene & recommendations

Audits & problem identification

Risk prevention service

Oenological products

Barrel hygiene

Wine treatment

www.thaleswcs.co.za

natamycin a oelofse

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