Molecular Surface Engineering

Short Introduction to PolyAn-Technology and Business Fields

- Molecular Surface Engineering (MSE):

systematic modification of a variety of

surface materials on molecular level

- Membranes for Pervaporation & Gasseparation

- Reactive surfaces for screening, diagnostics &

synthesis (e.g. biochips, microplates)

- Antifouling & biocompatible coatings for medical

devices & biomedical applications

Overview

CompanyCompany Business ModelBusiness Model

Customers & MarketsCustomers & Markets Technology & ProductsTechnology & Products

– A Technology & Service Provider Specialized in

Surface Modification and Functionalization for

Life Sciences & Industrial Applications

– R & D Services

– Industrial Cooperation

– Production and Manufacturing

– Licensing

– Chemical & Petrochemical Industry

– Pharmaceutics & Biotechnology

– Medical Devices & Laptools

– Food & Beverage Industry

– Sector: Nanotechnology

– GmbH, VC-Capital

– Foundation: 1996

– Employees :15

– Location: Berlin

– Revenue: 0,5 Mio € / a

R&D Network and Know-how

- Plastics and casting producer

- Pharmaceutical companies

- Refineries

- Toolbuilding companies

- Biotech-Companies

Patent portfolio

5 patents granted, 25 patents pending

Advisory BoardAdvisory Board AcademiaAcademia CooperationsCooperations

- Prof. Dr. Kleemann (Former

Board Member Asta-Medica)

- Prof. Dr. Wandrey (Director

Institute of Biotechnology,

Forschungszentrum Jülich)

- Prof. Dr. Schomäcker (Institute of

Technical Chemistry, Technische

Universität Berlin)

- Dr. Schellhaas (Director Sanimed

GmbH)

- Humboldt-Univ. Berlin

- Techn. Univ. Berlin

- Fraunhofer Institut

- GKSS Geesthacht

- IPF Dresden

- BAM Berlin

Application of Functionalized Materials - Examples

PolyAn‘s Molecular Surface Engineering - MSE Technology

Surface materials

> All kinds of polymers (e.g. polypropylene)

> Biodegradable polymers (e.g. polylactate)

> Metal and glass in combination with

polymers

Advantages

> Able to be applied to virtually any material

> High selectivity and stability of 3-dimensional functional

matrix

> Improved antifouling and biocompatible properties

> Switchable surface properties

> Combining the device properties with the properties of

the functional matrix

MSE on molecular level:

Functional matrixFunctional matrix Morphology of functional matrixMorphology of functional matrix

SubstrateSubstrate Morphology of substrateMorphology of substrate

MSE

„Tool Box“

Functional Groups:

-COOH -SO3

-SH / -SR

-OH / -OR -NH2

-NR3

-CHO

Immobilised molecules:

- Peptides - Saccharides

- Proteins - Lipides

- DNA / RNA - API

Artificial polymers:

- PP, PE, PES, PVDF, PS, PMMA,…

Biodegradable polymers:

- Polylactate - Cellulose

Inorganic materials:

- Glass - Metal oxides

- monolayers

- dense short chains (“brushes“)

- chains of variable length (tentaculars)

- dendritic structures

- molecular imprints (imprinting)

- molecular networks (hydrogels)

- dense, crosslinked films / layers

- flat surfaces

- porous materials (membranes)

- fibres

- particles

-

+

Functional matrixFunctional matrix Morphology of functional matrixMorphology of functional matrix

SubstrateSubstrate Morphology of substrateMorphology of substrate

MSE

„Tool Box“

Functional Groups:

-COOH -SO3

-SH / -SR

-OH / -OR -NH2

-NR3

-CHO

Immobilised molecules:

- Peptides - Saccharides

- Proteins - Lipides

- DNA / RNA - API

Artificial polymers:

- PP, PE, PES, PVDF, PS, PMMA,…

Biodegradable polymers:

- Polylactate - Cellulose

Inorganic materials:

- Glass - Metal oxides

- monolayers

- dense short chains (“brushes“)

- chains of variable length (tentaculars)

- dendritic structures

- molecular imprints (imprinting)

- molecular networks (hydrogels)

- dense, crosslinked films / layers

- flat surfaces

- porous materials (membranes)

- fibres

- particles

-

+

Molecular Surface Engineering – MSE „Tool Box“

Goal: PolyAn aims to generate innovative products for different application areas on basis of the

proprietary Molecular Surface Engineering technology in close cooperation with partners.

Market Segments

Principles of Used Technologies

Product Portfolio

Commercially available:

• Polymer chips: novel substrates for oligonucleotide synthesis

• Polymer-based biochips and microplates with unique reactive surfaces

for diagnostics and HTS

• Pervaporation membranes for different separation problems

• Membranes and particles, e. g. adsorbers, for bio-purification

• Functionalization services, „tailored” surfaces

Commercially available:

• Polymer chips: novel substrates for oligonucleotide synthesis

• Polymer-based biochips and microplates with unique reactive surfaces

for diagnostics and HTS

• Pervaporation membranes for different separation problems

• Membranes and particles, e. g. adsorbers, for bio-purification

• Functionalization services, „tailored” surfaces

Functionalized Labtools for in vitro-Diagnostics, High-throughput Screening and Cell Culture Example of the application of PolyAn‘s MSE technology

Innovation

> First high-throughput, polymer-based biochips

and microplates with unique reactive surfaces

for diverse applications (DNA, oligonucleotide,

protein, drug)

Advantages

> 3-dimensional, nanoporous polymer surface

with different functional groups

> Improved signal to noise ratio

> Excellent spot morphology

> High specific binding

Results

> Commercialization of biochips in 2004 and

manufacturing under ISO 9001:2000

Outlook

> Further enhancing product performance

by combination of reactive surfaces with

other coating features, e.g. immobilization

of a variety of biomolecules

> Other functionalities or functional groups

are available on request

Fluorescence

0

20

40

60

80

100

120

140

160

180

Quarz PMMA PolyAn

[re

l. f

luo

resc

en

ce]

Cy5 Cy3 FITC

Microstructures

spatially addressable

> No significant auto-fluorescence

caused by functionalization

> Adjustable surface properties

e.g. Hydrophilicity contact angle)

Innovation > Polymer-based biochips with unique reactive surfaces

Advantages > Generation of novel surface functionalities (e.g. poly-functional)

> Excellent signal-to-noise-ratio by combination of reactive surface and ultra-low

unspecific binding

Functionalized BiochipsSelected Results

HTA 96HTA 12

>High-throughput

biochips

Results

0

10

20

30

40

50

60

70

80

90

PMMA PolyAn MSE-slide

(HTA)

Microarray hydrophilic

Co

nta

ct

an

gle

(w

ate

r) [

°]

Pervaporation Membranes Example of the application of PolyAn‘s MSE technology

composite membranes by “pore-filling”

In situ formation and covalent

fixation of a thin, defect-free

polymeric functional film in the

separation layer of an

asymmetric UF membrane

Results

> High mechanical and chemical stability

of the composite membrane

> Pilot plant test successful

(aromatics/aliphatics separation):

excellent long-term stability under

industrial conditions

> Effective separation: high fluxes and

selectivities

Innovation

> High performance membranes for

special applications commercially

available (e. g. organo-selective)

Pervaporation Membranes Example of the application of PolyAn‘s MSE technology

> Improved membrane-efficiency (high flux

and selectivity, no swelling) through

ultra-thin polymeric functional layer

> Adjustable permeability / selectivity ratio

for specific needs by varying the

functional layer

> Tailored selectivity to specific

applications by designing the active

separation layer on the molecular level

(e.g. Alcohols, Aromatics, Other

Organics)

> Up-scale from industrial pilot plant to

industrial plant

Advantages of PolyAn pervaporation membrane

Outlook

> Continuous and automatic process (no

especially skilled personal necessary)

> Generates low recycling streams

> Easy and fast installation of the plant

technology by pre-mounted equipment

(e. g. modules)

> Operates directly with the liquid (or vapour)

components

> Direct separation of azeotropic mixtures

> No further components are introduced into

the process (e. g. entrainers)

> Very low energy costs - only the

evaporation heat for the removal of the

minor component necessary

Advantages of pervaporation processes

Industrial pilot plantaromatics/aliphatics

separation

Innovation

> Development of a new type of gasseparation and

vapour permeation membranes

> Knowledge-transfer from pervaporation to

gasseparation

Results

> PolyAn’s experience with gas selective

membranes:

> O2

/ N2

> CO2

/ N2

> CH4

/ N2

> Benzene / aromatics separation from organic

vapour

> Chlorinated compounds from hydrocarbons

Gasseparation, Vapour Permeation Example of the application of PolyAn‘s MSE technology

Outlook

> Solve further separation problems by

customization of membranes

> Up-scaling from lab-scale to pilot-plant

Advantages

> Tailor-made membranes

> Very good selectivities

> Long-term stability

Innovation

> New type of organophilic nanofiltration membranes (organic

solvent NF membranes)

> Knowledge-transfer from pervaporation / pore-filling concept to

nanofiltration

Results

> Customized membranes

> Solvent stable

> R&D-projects

Advantages

> Tailor-made membranes

> Very good selectivities

> Long-term stability

Nanofiltration MembranesExample of the application of PolyAn‘s MSE technology

Nanofiltration – lab-scale

Functionalized Materials for Bioseparation

Principles

1. Antifouling functionalization of membranes and particles

2. Selective binding

mild binding of biomolecules

- covalent coupling

- non-covalent coupling

→ intrinsic biological activity

is maintained

Principles

1. Antifouling functionalization of membranes and particles

2. Selective binding

mild binding of biomolecules

- covalent coupling

- non-covalent coupling

→ intrinsic biological activity

is maintained

Products

Reactive membranes and particlesfor covalent and non-covalentcoupling of biomolecules

• immobilization of:

- enzymes,

- antibodies,

- antigenes etc.

Ion exchange membranes

• capture capacity is comparable to

benchmarks and adjustable

• combination with ultra-low unspecific

background or other surface

properties

Separation membranes via hydrophobic interaction

• fine tuning of hydrophilicity /

hydrophobicity

• maximum of interaction between targets

or impurities

Products

Reactive membranes and particlesfor covalent and non-covalentcoupling of biomolecules

• immobilization of:

- enzymes,

- antibodies,

- antigenes etc.

Ion exchange membranes

• capture capacity is comparable to

benchmarks and adjustable

• combination with ultra-low unspecific

background or other surface

properties

Separation membranes via hydrophobic interaction

• fine tuning of hydrophilicity /

hydrophobicity

• maximum of interaction between targets

or impurities

Application

Diagnostics

• sample preparation

• detection

Down stream processing

• combination of process steps

• customized optimization of

membranes regarding target-

proteins or impurities

• time and cost reduction

Application

Diagnostics

• sample preparation

• detection

Down stream processing

• combination of process steps

• customized optimization of

membranes regarding target-

proteins or impurities

• time and cost reduction

Poly-An`s Roadmap

R&D-Service

Industry Cooperation

Production Licensing

-Basic R&D for

private and

public clients

and companies

-Activities in

public financed

development

projects and

networks

-Feasibility

studies for

companies

-Pilot plants and

applications

-Joint

development

activities

-Upscaling

-Small- and

middle sized

production for key

customers

-Customized

high-quality

production

-Value adding

production

- Licensing

agreements for

MSE technology in

manufacturing

processes

- Services related to

the MSE use at

production sites

- Long term

licensing

contracts

- Strategic

licensing

Contact

Jorge Lehr

Rudolf-Baschant-Str. 2

13086 Berlin

Tel.: +49 – (0)30 – 91 20 78 0

E-mail: mail@poly-an.de

www.poly-an.de

PolyAn GmbH