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  • www.mn-net.com

    www.mn-net.comMACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str. 68 52355 Dren GermanyFrance:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: sales-fr@mn-net.com

    Switzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: sales-ch@mn-net.com

    Germanyand international:Tel.: +49 24 21 969-0Fax: +49 24 21 969-199E-mail: info@mn-net.com

    MACHEREY-NAGEL

    EN ISO 9001: 2008CERTIFIED

    USA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: sales-us@mn-net.com

    Plas

    mid

    Pur

    ifica

    tion

    Gui

    de Plasmid purification productsfrom MACHEREY-NAGELMN guide to plasmid purification Find the optimal solutionNucleoBond

    NucleoSpin

    Superior yieldsOutstanding puritiesTime-saving procedures for reliable downstream applications

  • www.mn-net.com2

    NucleoSpinSilica-membrane technology .......................................NucleoSpin kits see page 6

    DNAcontaminants

    Sample lysis, release of DNA from cells, tissue

    DNA is bound to the silica membrane under high-salt conditions Interaction between DNA (hydrate shell is reversibly removed by chaotropic salt) and silica membrane

    Contaminants are washed away under high-salt and / or ethanolic conditions to keep the DNA bound to themembrane

    DNA is eluted in low-salt buffer or water, DNA is ready to use for downstream applications

    Principle of binding: Removing of hydrate shell Hydrogen bonds / Salt bridges

    Principle of elution: Reconstitution of hydrate shell

    Features / Results SequencingandPCR-gradeplasmidDNA Noalcoholprecipitationnecessary Fastandeasyprocedure

    MN technologies for plasmid purificationNucleoBond Anion-exchange technology ........................................NucleoBond kits see page 3

    DNAcontaminants

    DNA is bound to the anion-exchanger matrix under low-pH conditions Interaction between positively charged anion-exchanger group and negatively charged DNA backbone

    Stringent washing with increasing salt concentration to remove contaminants

    DNA is eluted with high-pH buffer

    Desalting / Concentration: Alcohol precipitation of eluted DNA DNA is collected by centrifugation or by using the NucleoBondFinalizer

    Si spacer NH

    CH3

    OHO

    CH2

    O

    P

    O

    O

    O

    anion-exchangergroup MAE

    DNA backbone

    binding

    Principle of binding: Ionic bond

    2

    3

    O

    CH

    O

    P

    O

    O

    O

    elution of DNA

    anion-exchangergroup MAE

    DNA backbone

    pH shift

    Si spacer N

    CH

    OH

    Principle of elution: pH shift

    Features / Results Ultra-pure,transfection-gradeplasmidDNA Thenewgenerationofanionexchangers Xtrafast,Xtrahighyield,Xtraconvenient

  • www.mn-net.com 3

    JJNucleoBond Xtra Midi NucleoBond Xtra Maxi

    Superior plasmid Midi and Maxi kits transfection-gradeXX Highest speed 30 min (Midi) / 35 min (Maxi) OptimalcolumndesignandinsertedfiltersavoidinconvenientsyringesXX Highest yield LyseControl for visualization of completed alkaline lysis Typically250gforMidiand1000gforMaxiXX High purity transfection-grade plasmid DNA

    Established anion-exchange technologyXX NucleoBond Xtra Plus kits for super high-speed version

    NucleoBondFinalizerforomittingtime-consumingcentrifugationafterDNAprecipitation

    Product at a glanceSample material Midi

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