mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase:...
TRANSCRIPT
Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase:
implications for load-bearing matrix growth and stability
by Amit P. Bhole, Brendan P. Flynn, Melody Liles, Nima Saeidi, Charles A. Dimarzio, and Jeffrey W. Ruberti
Philosophical Transactions AVolume 367(1902):3339-3362
September 13, 2009
©2009 by The Royal Society
(a) Schematic of the experimental setup used to carry out degradation of strained collagen under DIC microscopy.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
Contrast normalization and background noise removal.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
Time-series DIC and edge-detected ROI during both gelation and degradation of collagen.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
Representative plot showing quantification of edge loss against digestion time ΔT in control sample (no strain), where Istart is 95 per cent of (maximum edge detection intensity), Ifinish is 10
per cent of and ΔT is the time (s) between.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
DIC and edge-detected ROIs. (a) Intial strain on a heavily manipulated network of collagen fibrils.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
DIC and edge-detected ROIs. (a) Initial strain on a gently manipulated network of collagen fibrils.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
(a) Simulated fibril images (i) for diameters of 50, 150 and 250 nm and the result of the edge detection algorithm for each (ii).
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
Representative plot showing quantification of edge loss against digestion time ΔT, where Istart is 95 per cent of (maximum edge detection), Ifinish is 10 per cent of and ΔT is the time (s)
between.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
DIC images of the rapid degradation of the initially strained network shown in figure 2c.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
DIC and edge-detected ROIs in a similar experiment using MMP-8.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society
DIC image showing what appears to be a substantial accumulation of assembled collagen fibrils in the area of applied tensile strain on the network between the pipettes.
Amit P. Bhole et al. Phil. Trans. R. Soc. A 2009;367:3339-3362
©2009 by The Royal Society