lienhard pagel 4.6.2007 flow injection analysis realized using pcbs s. gassmann, l. pagel faculty of...

Lienhard Pagel4.6.2007

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Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronicsInstitute of Electronic Appliances and Circuits, University of Rostock, Rostock, Germany


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Micro fluidics in PCB technology

Electronic area IC's, resistor, capacitor, plug and socket connection, push-button, display, ...

Fluidic area:

pumps, valves, channels, filter, ... cavities, optical sensors, conductivity measurement, …

Electronic area

Fluidic area


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University of RostockPCB-Fluidics


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Fluidic PCB-Technology

normal PCB

production

drilling milling


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normal PCB

production

etching


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second PCB stacked

copper lines build channels

gluing


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tube connection

channel view


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cross section of channel


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Cross section of channel wall

epoxy

copper (2)

copper (1)channel


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9µC controlled programmable micro pump

Electrical connector- Power 4 –7 V DC- I2C - Bus - programming

500µl/min Flow 150 mBar max Backpressure

over USB-I2C IF fully programmable


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Flow-Injection-Analysis

quantitative measurement of components automated procedure short response times / high sample

throughput usage in quality control / automated

assays developed by J. Růžička and E.H. Hansen

in the year 1975


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Flow-Injection-Analysis

Sample

Reaction coil

Injection valve Detector

Reagent

Pump


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Reaction

Detection of Fe3+

Thiocyanate as reagent forms red colored Iron-Thiocyanate detection by absorption at 480 nm

SCNFe 33

3)(SCNFe


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Why a FIA?

All basic fluidic handling necessary- transportation of fluids- dosing of fluids- mixing- detecting- adoption to detection needed- electronics needed


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FIA in PCB-Technologie Principle

reagentpump

sample pump

reaction coil

injection detector

• 2 separate controllable pumps • no injection valve• optical detection

photometrical 480 nm


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MIXINGMIXINGFlow pattern in 180° curves


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Simulation results of the used reaction channel Simulation results of the used reaction channel

Parameters: 2 pumps with constant flow of 0.2ml/min

Fluidsimulation:FLOWORKS789388 cells


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… and the reality


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Simulation results of the used reaction channel Simulation results of the used reaction channel

parameters:thermopneumatically actuated pumps

with pulsing flow of 0.2ml/min,

789388 cells


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Simulation results of the reaction channelSimulation results of the reaction channel

vector plot of the fluid velocity at the cross section of the channel after the second curve

parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min,

789388 cells


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OPTICAL SENSOROPTICAL SENSOR in FIA

Pho to d io d e

O p tic a l p a th

1m m

Sim ula tio n Flo w 5 m l/m in!

LED


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Optical Cell with air bubble (vector of the force)Optical Cell with air bubble (vector of the force)

Sim ula tio n Flo w 5 m l/m in!

F


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Realization

5 cm

5 cm

• stack of four PCBs • one movable layer of polyimid• electronics on the back of the fourth PCB


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FIA Realization


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FIA in PCB with integrated electronics


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Signals in the photo detector in dependence on the concentration of Fe(III)ions


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PCB FIA facts

Detection Fe3+ Ions0,16 mMol – 10 mMolreproducibility peak height 5%, peak area 10%

Monolithic system consisting fluidics and electronics in one stack of PCBs.


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Thank you for your attention!Thank you for your attention!

lienhard pagel 4.6.2007 flow injection analysis realized using pcbs s. gassmann, l. pagel faculty of...

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