JOURNAL OF BACTERIOLOGYVOLUME 166 * JUNE 1986 NUMBER 3

Simon Silver, Editor in Chief (1987)Washington UniversitySt. Louis, Mo.

James D. Friesen, Editor (1987)University of TorontoToronto, Canada

Stanley C. Holt, Editor (1987)The University of Texas HealthScience Center, San Antonio

Samuel Kaplan, Editor (1988)University of Illinois, Urbana

June J. Lascelles, Editor (1989)University of California, Los Angeles

Richard M. Losick, Editor (1988)Harvard UniversityCambridge, Mass.

L. Nicholas Ornston, Editor (1987)Yale UniversityNew Haven, Conn.

Robert H. Rownd, Editor (1990)Northwestern Medical SchoolChicago, Ill.Graham C. Walker, Editor (1990)Massachusetts Institute of

TechnologyCambridge, Mass.

Robert A. Weisberg, Editor (1990)National Institute of Child

Health and HumanDevelopment

Bethesda, Md.

EDITORIAL BOARDDavid Apirion (1988)Stuart J. Austin (1987)Frederick M. Ausubel (1986)Gad Avigad (1986)Barbara Bachmann (1987)Manfred E. Bayer (1988)Margret H. Bayer (1986)Claire M. Berg (1986)Helmut Bertrand (1988)Terry J. Beveridge (1988)Edwin Boatman (1986)Donald A. Bryant (1988)Joseph M. Calvo (1987)A. M. Chakrabarty (1986)Keith F. Chater (1988)Terence G. Cooper (1987)John E. Cronan, Jr. (1986)Jorge H. Crosa (1988)Walter B. Dempsey (1986)Patrick Dennis (1988)W. Ford Doolittle (1988)David A. Dubnau (1986)Martin Dworkin (1988)Bert Ely (1988)

Wolfgang Epstein (1987)David Figurski (1987)Timothy J. Foster (1986)Robert T. Fraley (1988)Masamitsu Futai (1988)Robert Gennis (1988)Jane Gibson (1988)Robert D. Goldman (1988)E. Peter Greenberg (1988)Scott R. Hagedorn (1988)Barry G. Hall (1988)R. E. W. Hancock (1987)Richard S. Hanson (1988)Robert Haselkorn (1987)Gerald L. Hazelbauer (1987)Dennis Henner (1988)Charles L. Hershbberger (1987)Penny J. Hitchcock (1987)James Hopper (1988)Martha M. Howe (1987)Karin Ihler (1987)A. W. B. Johnston (1986)Eva R. Kashket (1987)David E. Kennell (1988)

Wil N. Konings (1987)Jordan Konisky (1987)Dennis J. Kopecko (1987)Vii Krishnapillai (1988)Terry Krulwich (1987)Lasse Lindahl (1987)Jack London (1987)Paul S. Lovett (1987)Ben J. J. Lugtenberg (1986)Robert Macnab (1988)Francis L. Macrina (1986)Sarah A. McIntire (1986)Larry McKay (1987)S. Mizushima (1988)Edward A. Morgan (1987)Gisela Mosig (1988)R. G. E. Murray (1986)Robert A. Niederman (1988)Allen T. Phillips (1988)Patrick J. Piggot (1987)Barry Polisky (1987)Linda Randall (1987)Marilyn Roberts (1986)

Palmer Rogers (1987)Barry P. Rosen (1986)Rudiger Schmitt (1986)June R. Scott (1987)Jane K. Setlow (1987)Peter Setlow (1987)James A. Shapiro (1988)Lucille Shapiro (1986)Louis A. Sherman (1988)Howard A. Shuman (1988)Issar Smith (1987)John L. Spudich (1988)Catherine Squires (1987)Robert Switzer (1987)Alexander Tomasz (1986)Godfried D. Vogels (1987)Barry Wanner (1987)Bernard Weisblum (1986)Malcolm Winkler (1988)David Womble (1986)Henry C. Wu (1987)Duane C. Yoch (1986)Howard Zalkin (1988)

Helen R. Whiteley, Chairman, Publications BoardLinda M. Illig, Managing Editor, Journals

Kirk Jensen, Director of PublicationsDennis G. Burke, Production Editor

The Journal of Bacteriology (ISSN 0021-9193), a publication of the American Society for Microbiology, 1913 I St., NWWashington, DC 20006, is devoted to the advancement and dissemination offundamental knowledge concerning bacteria andother microorganisms. Instructions to authors are published in the January issue each year; reprints are available from theeditors and the Publications Department. The Journal is published monthly, and the twelve numbers are divided into fourvolumes per year. The nonmember subscription price is $280 per year; single copies are $21. The member subscription price is$41 (foreign, $54 [surface rate]) per year; single copies are $7. Correspondence relating to subscriptions, reprints, defectivecopies, availability of back issues, lost or late proofs, disposition of submitted manuscripts, and general editorial mattersshould be directed to the ASM Publications Department, 1913 I St., NW, Washington, DC 20006 (phone: 202 833-9680).

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Second-class postage paid at Washington, DC 20006, and at additional mailing offices.POSTMASTER: Send address changes to Journal ofBacteriology, ASM, 1913 I St., NW, Washington, DC 20006.Made in the United States of America.Copyright © 1986, American Society for Microbiology.All Rights Reserved. a 11U(S'I'The code at the top of the first page of an article in this journal indicates the copyright owner's consent that copies of the arti-cle may be made for personal use or for personal use of specific clients. This consent is given on the condition, however, thatthe copier pay the stated per-copy fee through the Copyright Clearance Center Inc., 21 Congress St., Salem, MA 01970, forcopying beyond that permitted by Sections 107 and 108 of the U.S. Copyright Law. This consent does not extend to otherkinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collectiveworks, or for resale.

Author IndexAguirre, Jesuis, 1040Aldea, Marti, 985Aramaki, Hironori, 1089Asai, Yoshio, 739Ayala, Juan A., 985Aymerich, Stephane, 993

Beckwith, Jon, 878Beppu, Teruhiko, 937Berry, Denise, 1134Bhagwat, Ashok S., 751Bjork, Glenn R., 1013, 1022Blanco, Manuel, 1141Boos, Winfried, 706, 884Bouadloun, Fares, 1022Bove, Joseph M., 824Breidt, Frederick, Jr., 1061Bridges, Bryn A., 1141Bukau, Bernd, 706, 884Burgers, Peter M. J., 905Buu, Antoinette, 1137

Caffrey, Patrick, 1072Calvo, Joseph M., 1113Carman, George M., 779Case, Casey C., 706Cass, Laura G., 892Chapon, Christine, 1083Cohen, Stanley N., 999Collins, Mary Lynne Perille,

818Costanzi, Italo, 1055Cronan, John E., Jr., 872

D'Ari, Richard, 733Davidson, Victor L., 812Decker, Debra, 801de Pedro, Miguel A., 985Deutscher, Josef, 829Doherty, Helen, 1072Donadio, Stefano, 1055

Earhart, Charles F., 930Ehrmann, Michael, 884Eoyang, Lillian, 901Ericson, Johanna U., 1013

Fast, Robert, 857Fernandez, Serafin, 779Finlay, B. Brett, 713Fukazawa, Tsuyoshi, 722Fukuda, Masako, 1118

Gadeau, Alain-Pierre, 824Garduno, Fermin, 801Gemmill, Robert M., 1113Gertman, Eva, 1134Getty, Colin, 959

Gibson, David T., 1028Gonzy-Treboul, Genevieve,

993Granett, Sandra, 706Grogan, Dennis W., 872Guest, John R., 1067Guild, Walter R., 972, 978Gunsalus, l. C., 1089Guthrie, Ellen P., 959, 966

Hageman, Judith M., 972Hansberg, Wilhelm, 1040Harada, Shigeyoshi, 787Hashiguchi, Ken-Ichi, 787Hatfull, Graham F., 746Haughn, George W., 1113Henry, Susan A., 779Herrera, Guadalupe, 1141Hirano, Masanori, 945Hirata, Haruhisa, 722Hitchcock, Penny J., 699Homann, Michael J., 779Horikoshi, Koki, 728Horiuchi, Tadao, 1089Husain, Mazhar, 812

laccarino, Maurizio, 1055Imai, Mutsuo, 945Inouye, Sachiye, 739Isaksson, Leif A., 1022Ito, Koreaki, 849Ivey, D. Mack, 1128

Jackowski, Suzanne, 866Jensen, Kaj Frank, 857Joyce, Catherine M., 746

Kainuma, Keiji, 1118Karlstrom, Olle, 857Kato, Chiaki, 728Klein, Michael B., 905Kobayashi, Shoichi, 1118Kobayashi, Tetsuo, 728Koga, Hideo, 1089Koshland, Daniel E., Jr., 1007Koyama, Nobuto, 787Kropinski, Andrew M., 1134Kudo, Toshiaki, 728Kuo, Scot C., 1007Kwart, Lawrence D., 1028Kwon-Chung, Kyung Joo, 924

Lampen, J. O., 769Larsen, J0rgen Nedergaard,

857Laten, Howard M., 763Lee, Catherine A., 878Leive, Loretta, 699

Lindgren, Peter B., 1096Ljungdahl, Lars G., 1128Lowe, Michael, 756Lutkenhaus, Joe, 733, 756

Madura, Kiran, 914Magiun, Emmanuelle, 733Magnusson, Kerstin, 1067Maier, Robert J., 795Makela, P. Helena, 699Matsumoto, Yoshihiro, 945Mayer, Frank, 1128McCombie, W. Richard, 1028Menichi, Bernadette, 1137Messner, Paul, 1046Monma, Mitsuru, 1118Mori, Masaki, 787Morrison, David C., 699Moshiri, Farhad, 795Mouches, Claude, 824Myers, Charles R., 818

Nakazawa, Atsushi, 739Nakazawa, Teruko, 739Negoro, Seiji, 722Neher, Jerryl W., 812

Obinata, Masuo, 787Okada, Hirosuke, 722Omer, Charles A., 999Owen, Peter, 1072

Paladino, Renato, 1055Palchaudhuri, Sunil, 1106Panopoulos, Nickolas J., 1096Paranchych, William, 713Peet, Richard C., 1096Philips, Mary K., 1067Pierce, James R., 930Pollock, Thomas J., 801Pozzi, Gianni, 972Prakash, Satya, 914Priebe, Scott D., 972, 978Prpic, J. Kaya, 1131Pugsley, Anthony P., 1083Pum, Dietmar, 1046

Rietschel, Ernst T., 699Roberts, Richard J., 751Robins-Browne, Roy M., 1131Rock, Charles O., 866Ruiz-Rubio, Manuel, 1141Rutberg, Lars, 1067

Salerno, Anthony J., 769Salyers, Abigail A., 959, 966Sanjanwala, Bharati, 756Sara, Margit, 1046

Sauerwald, Helga, 829Schreil, Werner, 1055Schwartz, Maxime, 1083Shiba, Kiyotaka, 849Shigesada, Katsuya, 945Shimkets, Lawrence J., 837,

842Shoemaker, Nadja B., 959Silverman, Philip M., 901Sleytr, Uwe B., 1046Sohail, Anjum, 751Sparapani, Piero, 1055Srichaiyo, Tasanee, 1022Steinmetz, Michel, 993Stetter, Karl O., 1046Stewart, George C., 1061Strittmatter, Wolfgang, 699Stuart, Simon J., 1131Stults, Larry W., 795

Takano, Toshiya, 1118Takeuchi, Koji, 1089Tamura, Gakuzo, 787Tanimoto, Akihiko, 787Thompson, Ted, 801Thorne, Linda, 801Tormo, Antonio, 985Trumbly, Robert J., 1123

Uozumi, Takeshi, 937

Valentine, Peter J., 763Van Kast, Carol A., 763Vicente, Miguel, 985Vijayakumar, Moses N., 972,

978Villarejo, Merna R., 706

Walfield, Alan M., 801Weber, Peter C., 1106Wessler, Susan R., 1113Whelan, William L., 924White, Bradley N., 1134Whited, Gregory M, 1028Wilcox, Gary, 892Wild, Martha, 801Willis, David K., 1096Woodgate, Roger, 1141

Yamada, Hisami, 1072Yamaguchi, Eiichi, 1089Yamane, Kunio, 1118Yamasaki, Makari, 787Yanagida, Noboru, 937Yoda, Koji, 787Yura, Takashi, 849

Zounes, Maryann, 801

AUTHOR INDEX

VOLUME 166

Aguirre, Jesus, 1040Aizawa, Shin-Ichi, 187Aldea, Marti, 985Allen, E. Randall, 1Altendorf, Karlheinz, 334Anderson, Beau J., 453Antonucci, Tammy K., 565Aramaki, Hironori, 1089Armstrong, Sandra K., 212Asai, Yoshio, 739Ayala, Juan A., 985Aymerich, Stephane, 993

Baldwin, W. W., 435Barnes, Eugene M., Jr., 281Barras, Frederic, 346Bateman, Joseph N., 155Beacham, Ifor R., 135Beckwith, Jon, 505, 878Belet, Monique, 15Bennett, Michael F., 38Benson, David R., 301Benson, Terry A., 194Beppu, Teruhiko, 937Berry, Denise, 1134Beveridge, Terry J., 120Bhagwat, Ashok S., 751Bjork, Glenn R., 1013, 1022Blackhart, Brian D., 673Blanco, Manuel, 1141Bloom, Mark, 380Boos, Winfried, 706, 884Bouadloun, Fares, 1022Bove, Joseph M., 824Braun, Volkmar, 404Breidt, Frederick, Jr., 1061Brey, Robert N., 51Bridges, Bryn A., 1141Brill, Winston J., 148Broderick, Stephanie H., 399Brot, Nathan, 380Brown, James W., 686Brunton, James L., 375Buck, Martin, 545Bukau, Bernd, 706, 884Burgers, Peter M. J., 905Burgess, Barbara K., 180Burns, Dennis M., 135Buu, Antoinette,' 1137

Caffrey, Patrick, 1072Calvo, Joseph M., 341, 1113Carman, George M., 779Carmona, Cynthia, 644Carraway, M., 29Case, Casey C., 706Cass, Laura G., 892Casuto, Dianne, 194Chandra, T.' S., 446Chapon, Christine, 1083Charon, Nyles W., 105Chaudhry, G. Rasul, 238Chen, E., 113Chen, Jiann-Shin, 162Chen, Katherine Chuan-Kai,

162Chilton, Mary-Dell, 491Chippaux, Marc, 346Cohen, Stanley N., 999

Collins, Mary Lynne Perille,818

Congi, Rosa, 375Conners, Michael J., 412, 417Costanzi, Italo, 1055Cox, Betty Jo, 349Cronan, John E., Jr., 872

Dang, Chuan V., 275D'Ari, Richard, 733Davidson, Victor L., 812Davis, Bernard D., 439Dean, Dennis R., 180Dean, Gary E., 244Decker, Debra, 801de Graaf,'Frits K., 260De Grandis, Stephanie, 375de Jonge, Abraham J. R., 260Demarez, Marc, 44de Pedro, Miguel A., 985Dessaux, Yves, 44Deutscher, Josef, 829de Vries, Petra, 260Dibb, N. J., 83Dilts, Judith A., 349Doherty, Helen, 1072Donadio, Stefano, 1055Downie, J. A., 552Durbin, Richard D., 224

Earhart, Charles F., 930Egerton, John R., 453Ehrmann, Michael, 884Ekiel, Irena, 559Emr, Scott D., 313Eoyang, Lillian, 901Epstein, Wolfgang, 334Ericson, Johanna U., 1013Erratt, J. A., 484Evans, J. D., 72

Fast, Robert, 857Feduik, Linda, 155Ferenci, T., 95Fernandez, Serafin, 779Ferrari, E., 113Ferrari, Eugenio, 173Ferry, James G., 500Fett, W. F., 66Field, Charles, 313Finlay, B. Brett, 368, 713Fischer, Eckhard, 404Fishman, M. L., 66Flores, Enrique, 693Foster, John W., 666Fournier, Maurille J., 341Franke, Jakob, 290Freese, Ernst, 1Frey, Joachim, 15Friedrich, Cornelius G., 446Friesen, James, 375Froehlich, Barbara J., 609Frost, Laura S., 368Fujita, Hiroshi, 187Fukazawa, Tsuyoshi, 722Fukuda, Masako, 1118Furukawa, Kensuke, 338, 392

Gadeau, Alain-Pierre, 824Garduno, Fermin, 801

Gemmill, Robert M., 1113George, Susan E., 533Gertman, Eva, 1134Getty, Colin, 959'Ghersa, Paola, 15Gibson, David T., 1028Glass, N. Louise, 598Gober, James W., 618Golub, Efim I., 670CGonzy-Treboul, Genevieve,

993Goulbourne, E., Jr., 59Gowrishankar, J., 331Grambow, Nancy, 341Granett, Sandra, 706Gray, Gregory L., 644Green, Christopher J., 306Gregory, Eugene M., 528Grogan, Dennis W., 872Grothe, Suzanne, 253Guest, John R., 1067Guild, Walter R., 972, 978Gundling, Gerard J., 194Gunsalus, l. C., 1089Guthrie, Ellen P., 959, 966

Hageman, Judith M., 972Haldenwang, William G., 472Halfmann, G., 683Halperin, Walter, 88Hamasaki, Hidehisa, 128Hancock, Robert E. W., 212Handelsman, Jo, 148Hansberg, Wilhelm, 1040Harada, Shigeyoshi, 787Hartline, Richard A., 155Hashiguchi, Ken-Ichi, 787Hatfull, Graham F., 746Haughn, George W., 1113Henner, Dennis J., 113, 461Henry, Susan A., 779Herrera, Guadalupe, 1141Hewlett, Erik L., 230Hildebrand, Eilo, 689Hiraga, Sota, 100Hirano, Masanori, 945Hirata, Haruhisa, 722Hirose, Seiyu, 128Hitchcock, Penny J., 699Hoch, James A., 173, 412Hoffmann, Helmut, 404Homann, Michael J., 779Horikoshi, Koki, 728Horiuchi, Tadao, 1089Hoshino, Takayuki, 338Howard, Sandra M. H., 173,412

Huang, Annie, 375Husain, Mazhar, 812

Iaccarino, Maurizio, 1055Iandolo, John J., 385,' 574Igarashi, Kazuei, 128Imai, Mutsuo, 945Imlay, James A., 519Inouye, Sachiye, 739Isaksson, Leif A., 1022Ishihara, Akira, 187Ito, Koreaki, 849Ivey, D. Mack, 1128

Jackowski, Suzanne, 866Jackson, Matthew P., 574Jaffe, Aline, 100Jayakumar, A., 281Jen, George C., 491Jensen, Kaj Frank, 857Jerlstrom, Pierre G., 135Johnson, John L., 162Johnson, L. P., 623Jones, Christopher L., 34Jones, Judith W., 341Joyce, Catherine M., 746

Kadam, Sunil K., 651Kainuma, Keiji, 1118Kakegawa, Tomohito, 128Karlstrom, Olle, 857Karmali, Mohammed, 375Kashiwagi, Keiko, 128Kashket, Eva R., 618Kato, Chiaki, 728Kelley, R. L., 269Kessin, Richard H., 290Khan, Saleem A., 34Kindle, Karen L., 644Klein, Mishael B., 905Knight, C. D., 552Knight, Thomas J., 224Knox, K. W., 72Kobayashi, Shoichi, 1118Kobayashi, Tetsuo, 728Koga, Hideo, 1089Kogoma, Tokio, 361Komari, Toshihiko, 88Kong, Qui-Tong, 353Koningstein, Gregory, 260Koshland, Daniel E., Jr., 1007Kosuge, Tsune, 598Koyama, Nobuto, 787Koyama, YoshinQri, 338Kraut, Hildegard, 404Kristo, Craig L., 453Krogsrud, Richard L., 253Krone, Willy J. A., 260Kropinski, Andrew M., 1134Kudo, Toshiaki, 728Kumamoto, Carol A., 505Kuo, Scot C., 1007Kuroda, Mitzi I., 461Kwart, Lawrence D., 1028Kwon-Chung, Kyung Joo, 924

Lampel, Keith A., 238Lampen, J. O., 769Lampson, Bert C., 479Lamsa, Michael H., 644Landick, Robert, 217, 565Langston-Unkefer, Pat J., 224Larsen, J0rgen Nedergaard,'857

Laten, Howard M., 763Lee, Catherine A., 878Lee, Chia Yen, 385Lee, K.-S., 95LeGall, J., 604Legrain, Christiane, 44Leive, Loretta, 699

i

ii AUTHOR INDEX

Lepelletier, Michele, 346Li, Zhi-Gang, 357Lidstrom, Mary E., 581, 591Limberger, Ronald J., 105Lindgren, Peter B., 1096Lindler, Luther E., 658Linn, Stuart, 519Liu, M.-C., 604Liu, M.-Y., 604Ljungdahl, Lars G., 1128Lohmanp, Annemarie, 334Longtin, D., 628Low, K. Brooks, 670Lowe, Michael, 756Lowery, Robert G., 513Lubitz, W., 683Lucchesi, Pamela, 29Ludden, Paul W., 513Luger, Selina M., 439Lunsford, R. Dwayne, 426Luque, F., 541Lutkenhaus, Joe, 733, 756

Ma, Zhi-Fang, 353Macnab, Robert M., 187, 244MacNeil, Douglas, 281Macrina, Francis L., 426, 658Madrzak, C., 628Madura, Kiran, 914Maeba, Peter Y., 635Magiun, Emmanuelle, 733Magnusson, Kerstin, 1067Maier, Robert J., 795Mainzer, Stanley E., 644Makela, P. Helena, 699Marinus, M. G., 29Mason, James M., 4i7Matin, A., 59Matin, M., 59Matsumoto, Yoshihiro, 945Matsumura, Philip, 244Matsuzaki, Shigeru, 128Mattick, John S., 453May, Harold D., 500Mayer, Frank, 1128McClellan, Denise J., 253McCombie, W. Richard, 1028Medina, J. R., 541Melton, Thoyd, 533Menichi, Bernadette, 1137Messner, Paul, 1046Mickel, F. Susan, 78Miller, Mahlon F., 194Milner, Jocelyn L., 253Miura, Atsuko, 128Miyazaki, Toshitsugu, 392Monma, Mitsuru, 1118Mori, Hirotada, 100Mori, Masaki, 787Morrison, David C., 699Mpshiri, Farhad, 795Mouches, Claude, 824Moyed, Harris S., 399Musser, James M., 230Myers, Charles R., 818

Nagami, Yoichi, 20Naito, Satoshi, 143Nakazawa, Atsushi, 739Nakazawa, Teruko, 739Nasim, A., 484

Nazos, Penelope M., 565Negoro, Seiji, 722Neher, Jerryl W., 812Neidhardt, Frederick, 380Nester, Eugene W., 88Nikawa, Jun-ichi, 328Niwano, Mitsuru, 275Noridge, Nancy A., 301Nunn, David N., 581, 591

Obinata, Masuo, 787Ogura, Teru, 100Okada, Hirosuke, 722Omer, Charles A., 999Orrego, Cristian, 1Osman, S. F., 66Oudega, Bauke, 260Owen, Peter, 1072Oxender, Dale L., 565

Palacios, Paul G., 15Paladino, Renato, 1055Palchaudhuri, Sunil, 1106Panopoulos, Nickolas J., 1096Paranchych, William, 368, 713Parisi, Joseph T., 479Parker, Charlotte D., 212Parr, Thomas R., Jr., 212Patte, Jean-Claude, 297Payne, W. J., 604Peet, Richard C., 1096Pennington, Charles D., 528Peppler, Mark S., 230Petit, Annik, 44Petric, Martin, 375Philips, Mary K., 1067Pierce, James R., 930Pollock, Thomas J., 801Pozzi, Gianni, 972Prakash, Satya, 914Priebe, Scott D., 972, 978Prpic, J. Kaya, 1131Pugsley, Anthony P., 1083Pum, Dietmar, 1046

Quackenbush, Robett L., 349

Ramaley, Robert F., 238Rao, N. N., 205Ratet, Pascal, 297Ray, G. Luann, 472Reddy, C. Adinarayana, 269Reeve, John N., 686Rehemtulla, Alnawaz, 651Requadt, Carol, 644Rey, Michael W., 644Richaud, Catherine, 297Richaud, Francois, 297Rietschtel, Ernst T., 699Roberts, Richard J., 751Robertson, J. G., 552Robins-Browne, Roy M., 1131Robinson, Amy C., 180Rock, Charles O., 866Rossen, L., 552Rudikoff, Stuart, 238Ruiz-Rubio, Manuel, 1141Rutberg, Lars, 1067Ryu, Jun-ichi, 275

Saari, Leonard L., 513Salerno, Anthony J., 769Salyers, Abigail A., 959, 966Sanderson, Kenneth E., 651Sanjanwala, Bharati, 756Santero, E., 541Sara, Margit, 1046Sargent, Michael G., 38Sauerwald, Helga, 829Schauer, Neil L., 500Schekman, Randy, 313Schell, Mark A., 9Schimz, Angelika, 689Schlievert, P. M., 623Schmetterer, Georg, 693Schreil, Werner, 1055Schulman, I., 281Schwartz, Maxime, 1083Scott, June R., 609Searles, Lillie L., 341Selander, Robert K., 230Setlow, Peter, 412, 417Shen, San-Chiun, 353, 357Shen, Szi-Shih, 357Shiba, Kiyotaka, 849Shigesada, Katsuya, 945Shimkets, Lawrence J., 837,

842Shimotsu, Hidenori, 461Shivakumar, Annapur G., 194Shoemaker, Nadja B., 959Silverman, Philip M., 901Skelly, Susan, 380Sleytr, Uwe B., 1046Smnith, Ian C. P., 559Sohail, Anjum, 751Sparapani, Piero, 1055Spear, Brian B., 194Spector, Michael P., 666Speer, Brenda, 155Spremulli, Linda L., 78Spring, Kevini J., 135Sprott, G. Dennis, 559Srichaiyo, Tasahee, 1022Stader, Joan, 244Stanley, J., 628Steinbiichel, Alexander, 319Steinmetz, Michel, 993Stetter, Karl O., 1046Stewart, George C., 1061Stewart, Murray, 120Stewart, Valley, 217Strauch, Kathryn L., 505Strittmatter, Wolfgang, 699Stuart, Simon J., 1131Stults, Larry W., 795Stuy, Johan H., 285

Tai, Phang C., 439Takahashi, Hidenori, 100Takano, Toghiya, 1118Takeuchi, Koji, 1089Tamura, Gakuzo, 787Tanaka, Teruo, 20Tanimoto, Akihiko, 787Tatchell, Kelly, 364Taylor, Barry L., 275Tempt, Jacqtles, 44Thompson, Ted, 801Thorne, Linda, 801Tomai, M. A., 623

Tomizuka, Noboru, 338Tormo, Antonio, 985Torriani, A., 205Tortolero, M., 541Trumbly, Robert J., 1123Trust, Trevor J., 120Tschopp, Juerg F., 313Tsukagoshi, Yuko, 328Tyler, Bonnie, 341

Uchida, Hisao, 143Ugalde, Rodolfo A., 148Uozumi, Takeshi, 937Uratani, Brenda, 238

Vacante, Dominick, 244Valentine, Peter J., 763VanBogelen, Ruth, 380Van Kast, Carol A., 763Verma, D. P. S., 628Vicente, Miguel, 985Vijayakumar, Moses N., 972,

978Villarejo, Merna R., 706Vold, Barbara S., 306

Wabiko, Hiroetsu, 1Walfield, Alan M., 801Wang, E., 205Wang, Li-Wen, 357Watkins, Charles, 609Weber, Peter C., 1106Wegener, W. S., 435Weiss, Richard L., 679Weissbach, Herbert, 380Wells, B., 552Wender, Paul E., 9Wessler, Susan R., 1113Whelan, William L., 924White, Bradley N., 1134Whited, Gregory M, 1028Wiame, Jean-Marie, 44Wicken, A. J., 72Wilcox, Gary, 892Wild, Martha, 801Willis, David K., 1096Wolf, Julie B., 51Wolfe, P. B., 83Wood, Janet M., 253Woodgate, Roger, 1141Wu, Qi-Long, 353

Yamada, Hisami, 1072Yamaguchi, Eiichi, 1089Yamaguchi, Shigeru, 187Yamane, Kunio, 1118Yamasaki, Makari, 787Yamashita, Satoshi, 328Yanagida, Noboru, 937Yang, M., 113Yanofsky, Charles, 217, 461Yashphe, J., 205Yoda, Koji, 787Yura, Takashi, 849

Zerez, Charles R., 679Zhu, Jia-Bi, 357Zounes, Maryann, 801Zusman, David R., 673Zychlinsky, E., 59

J. BACTERIOL.

SUBJECT INDEXVOLUME 166

Acetohydroxyacid synthase IE. coli

IlvN polypeptide subunit, role, 901valine inhibition sensitivity, 901

"Achromobacter cycloclastes"copper proteins

electron transfer specificity, 604properties, 604

Acidophilic bacterium PW2bioenergetic parameters, 59A&C maintenance, 59ApH maintenance, 59

AdenosineS. cerevisiae

accumulation and deamination, 763effect of adenine levels, 763

Adenylate cyclaseB. pertussis

calmodulin requirements, 290partial purification, 290secretion, 290

ADP-ribosylationR. rubrum

nitrogenase iron protein regulation,513

Aerobactin production by Yersinia spp.,1131

Aeromonas salmonicidaA-layer patterns

permeability, 120structural transformation, 120

permeabilityA-layer patterns, 120

AerotaxisE. coli

inversion in cheB mutants, 275Agrobacterium tumefaciens

arginine catabolismTi plasmid, role, 44

mini-T plasmidsplant cell transformation, 491T-DNA borders, activity, 491

tumor-inducing plasmid pTiBoS42map, 88supervirulence, 88

A-layer patternsA. salmonicida

permeability, 120structural transformation, 120

Alcaligenes eutrophusE. coli pfkA gene expression, 319

Alkaline phosphataseE. coli pho regulon mutants, 205

Aminopropylcadaverineformation in E. coli, 128

Ammonium transportE. coliglnALG operon, role, 281glutamine synthetase mutants, 281

a-AmylaseB. subtilis hyperproduction, 787

z-Amylase structural genesB. licheniformis, 635B. stearoihermophilus, 635

Anacystis nidulansprotein synthesis elongation factors Tuand G, 78

Anion-selective porinB. pertussis

antibiotic uptake, 212Antibiotic uptake

B. pertussismajor outer meinbrane protein, 212

Arginine catabolismA. tumefaciens

role of Ti plasmid, 44L-Asparaginase

E. coliansA gene and product, 135

AutolysisE. coli

penicillin induction, 683phage 4~X174 gene E induction, 683

Azotobacter vinelandiichlorate-resistant mutants, 541nitrogenase structural gene mutants

nitrogenase activity, 180nitrogenase components, 180nitrogenase reconstitution, 180

ntrA-like mutantschlorate-resistant mutants, 541nitrogenase, nitrate, and nitrite defi-

ciency, 541

Bacillus licheniformis3-lactamase blaP genetranscriptional regulation, 769transcript synthesis, 769

thermophiic a-amylase gene, 635Bacillus macerans

cyclodextrin glucanotransferase geneexpression in B. subtilis, 1118molecular cloning, 1118nucleotide sequencing, 1118

Bacillus megateriumcobalamin biosynthesis-deficient mu-

tants, 51vitamin B12 growth requirement, 51

Bacillus natto DNA fragmentB. subtilis

levansucrase production, 20protease production, 20

Bacillus sp.penicillinase

excretion through E. coli outer mem-brane, 728

plasmid pMB9 kil gene, 728Bacillus stearothermophilusbgaB gene sequence and flanking re-

gion, 722,-galactosidase gene structure, 722a-glucans temperature-sensitive binding,

95novel survival mechanism, 95starch binding, 95thermophilic a-amylase gene, 635

Bacillus subtilisamyE-tmrB region

a-amylase hyperproductivity, 787gene amplification-inducing DNA, 787tunicamycin resistance, 787

ca-amylase hyperproductivity, 787B. macerans cyciodextrin

glucanotransferase geneDNA nucleotide sequencing, 1118expression, 1118molecular cloning, 1118

B. natto DNA fragmentlevansucrase production, 20protease production, 20

B. thuringiensis b-endotoxin genescrystal-like inclusions, 194

iii

vegetative expression, 194ethA mutationphage l105 DNA modification, 1sporulation induction, 1

glucose dehydrogenase genedevelopmental regulation, 238enzyme properties, 238location, 238nucleotide sequence, 238sporulating cells, 238

levansucrase sacB gene, 993membrane-binding sequence, 38membrane-particle-as§ociated DNA se-

quence, 38novel transcription attenuation, 461phage (105 DNA modification, 1pleiotropic sacQ

identification, 113protease expression, 113

purA, 38sacR 5'-noncoding region regulation,

993sdhA cytochrome b558 gerte

nucleotide sequence, 1067spore protein geneschromosonial location, 412cloning and sequencing, 417

sporulationstage 0 mutations, 173subtilisin expression, 173

sporulation-induced, DNA-dependentRNA polymerasein vitro- and in vivo-transcribed

genes, 472sporulation induction, 1ssp genes, 412, 417subtilisin expression

stage 0 sporulation mutations, 173succinate dehydrogenase complexcytochrome b558 gene, 1067sdh operon nucleotide sequence, 1067

tRNA genesE. coli expression, 306promoter function, 306

trp operon regulated termination, 461tryptophan operon expression regula-

tion, 461tunicamycin resistance, 787

Bacillus thuringiensivDiptera-specific insecticidal endotoxin,

8018-endotoxin genes

crystal-like inclusions, 194vegetative expression in B. subtilis,

194Lepidoptera-specific insecticidal

endotoxin, 801subspecies insecticidal endotoxin genes

structural similarity, 801Bacteriocin production and immunity

S. aureus, 574Bacteriophage4105 DNA modification

B. subtilis ethA, 1D3

IS222, 1134P. aeruginosa lipopolysaccharide,

1134H-19B

E. coli Shiga-like toxin, 375homology to phage X, 375

iv SUBJECT INDEX

L54aS. aureus geh lipase gene, 385

Mu dl and dl-8S. typhimurium psi loci, 666

P1 Ap Cmgene amplification, 609high-copy-number replicons, 609ISI-dependent generation, 609

P22MNNG-induced mutations, 34mnt repressor gene,, 34

R. meliloti phage sensitivity, 148S. erythreus

phage-resistant mutants, 1055T5

E. coli FhuA-peptidoglycan associa-tion, 1137

T12speA gene, 623streptococcal pyrogenic exotoxin A,

6234X174 gene E product

E. coli autolysis, 683Bacteroides nodosus

fimbriaebasan antigens, 453structural sUbunit, 453

Bacteroides spp.plasmids pBFTM10 arnd pB851

E. coli-Bacteroides shuttle vector mo-bilization, 959

Bacteroides thetaiotaomicronchondroitin sulfate utilization

chondroitin lyase II gene, 966targeted insertional mutagenesis, 966

superoxide dismutasesisolation and reconstitution, 528

Bioenergetic parametersacidophilesAC maintenance, 59ApH maintenance, 59

Biphenyl catabolismP. pseudoalcaligenes bph genes, 392

Bordetella pertussisadenylate cyclase secretion

calmodulin requirements, 290partial purification, two forms, 290

anion-selective porinantibiotic uptake pathway, 212lipid bilayer membrane channels, 212

major outer membrane porin protein,212

Bordetella spp.enzyme electrophoretic variation, 230genetic diversity and relationships, 230

Bradyrhizobium sp.proton motive forceK+ effects, 618membrane potential, 618nitrogen fixation effects, 618oxygen tension effects, 618

Cadmiutn resistanceS. aureus, 574

Caedibacter taeniospiralisR-body-coding plasmids, 349transposonlike elemnents, 349

Calmhodulin requirementB. pertussis

adenylate cyclase, 290Catabolite repression

S. cerevisiae irivertase synthesis, 1123Cation transport, 334

Cell elongationgram-negative bacteria, 435"Lineola longa"

kinetics, 435Chemolithoautotrophic-growth mutants

T. pantotropha, 446Chloramphenicol

E. coli sec mutantsgrowth and protein synthesis inhibi-

tion, 878Choline transport

S. cerevisiae gene, 328Chondroitin sulfate utilization

B. thetaiotaomicronchondroitin lyase II gene, 966targeted insertional mutagenesis, 966

Chromatophore membrane compositionR. tubrum

cell cycle-specific oscillation, 818protein/bacteriochlorophyll a ratio,

818protein/phospholipid ratio, 818

Cloacin DF13 uptakeE. coliimmunity protein removal, 260molecular fragmentation, 260

Clostridium pasteurianumnitrogenase genes

biased codon usage, 162nifHl-like gene structure, 162

Clostridium thermoaceticumF1-ATPasemacromolecular organization, 1128

membrane vesicles, 112813C nuclear magnetic resonariceH. cutirubrum mevalonic acid synthe-

sis, 559Cobalamin biosynthetic pathway

B. megaterium, 51Codon context sensitivity

suppressor tRNA translational accu-racy, 1022

Coenzyme AE. coli, content

acyl carrier protein production, 866consequences of reduction, 866phospholipid/protein ratio, 866saturated/unsaturated fatty acid ratio,

866ColEl DNA replication

E. coli, 143Colicin DColD plasmid, 15E. coli SOS regulatory system, 15

Copper proteins"A. cycloclastes"

electron transfer specificity, 604properties, 604

Cryptococcus neoformansgenetic complementation

formation of hyphal colonies, 924growth of prototrophic

heterokaryons, 924Cyclic AMP

E. coli csm mutations, 533Cyclodextrin glucanotransferaseB. macerans gene

expression in B. subtilis, 1118molecular cloning, 1118nucleotide sequencing, 1118

Cyclopropane fatty acidsE. coli

beneficial role(s) in cells, 872

synthesis-defective cfa mutants, 872Cytochrome b558

B. subtilis sdhA gene, 1067Cytochrome P-450c,,,

hydroxylase operoncamR negative regulator, 1089

Cytoplasmic membraneE. coliMotB site-limited incorporation, 244

Cytosine methylaseE. coli dcm locus, 751

Dihydrodipicolinate synthetaseE. coli dapA, 297

Electron transfer flavoproteinM. methylotrophus, 812

Electron transfer specificity"A. cycloclastes" copper proteins, 604

Elongation factors Tu and GA. nidulans genes, 78

8-Endotoxin genesB. thuringiensis

crystal-like inclusions, 194vegetative expression in B. subtilis,

194Enterobacter cloacae

nifA temperature sensitivity, 357Enterotoxin B

S. aureus entBnucleotide sequence, 29

Erwinia chrysanthemiclb expression in E. coligyrA mutations, effects, 346

Escherichia coliacetohydroxyacid synthase I

IlvN polypeptide subunit, 901valine inhibition sensitivity, 901

aerotaxisinversion in theB mutants, 275

,B-alanine auxotrophscellular coenzyme A content, 866

aminopropylcadaverine formation, 128ammonium transport regulationgInALG operon, role, 281glutamine synthetase mutants, 281

ansA gene and product, 135araBAD operon expression, 892AraC protein

activator function, 892ara binding sites, 892repressor function, 892

araC regulatory gene mutations, 892asparaginase-deficient mutants, 135bimodal hydrogen peroxide killing pat-

ternanoxically grown strains, 519DNA-repair-defective strains, 519

B. subtilis tRNA gene expression, 306ccd killing function, 100cell deathribosome degradation, role, 439

cfa null mutantscyclopropane fatty acid defect, 872

cheB mutantsinversion of aerotaxis, 275

cloacin DF13 uptakeimmunity protein removal, 260molecular fragmentation, 260

ColEl DNA replication, 143csm mutations

catabolite-repressible operon expres-sion, 533

J. BACTERIOL.

SUBJECT INDEX v

crp gene similarity, 533cyclic AMP suppressor mutation

catabolite-repressible operon expres-sion, 533

cyclopropane fatty acidsbeneficial role(s) in cells, 872synthesis-defective cfa mutants, 872

cytoplasmic membranemotB product incorporation, 244MotB site-limited incorporation, 244

dapAchromosomal location, 297nucleotide sequence, 297

dcm locuscloning and characterization, 751cytosine methylase, 751EcoRII recognition site modification,

751methylase product, 751

dihydrodipicolinate synthetase gene, 297DNA sequencesA+T content conservation, 1113

E. chrysanthemi clb expressiongyrA mutation, effect, 346

endogenously induced autolysispenicillin, 683phage 4X174 gene E product, 683

envelopesferrienterobactin uptake, 930

envZII missense mutationprotein synthesis repression, 706

excision-defective lexA51 mutants, 1141fep genes

enterobactin iron transport, 930FhuA proteins

lipoprotein requirement, 1137peptidoglycan association, 1137phage T5 receptor, 1137

FhuA receptor proteinoverproducing strain, 405plasmid pHK232 programming, 405preparation from cell envelopes, 405

flaA (cheC) locusflagellar-rotation directional control,

1007sequence, 1007transcription initiation site, 1007

F plasmid ced mechanism, 100ftsA mutations

penicillin effects, 985resistance to lysis, 985

FtsA proteinseptation, 985

generation of recombinant plasmidsS. pneumoniae DNA, 972

glnALG operonammonium transport regulation, 281

glutamine synthetase mutantsammonium transport regulation, 281

heat shock genesheat shock regulatory protein, effect,

380htpR gene product, 380

hipAcloning and expression, 399murein synthesis inhibition, 399persistence of expression, 399

intracellular coenzyme A contentacyl carrier protein production, 866consequences of reduction, 866phospholipid/protein ratio, 866saturated/unsaturated fatty acid ratio,

866

K+ transport systems, 334leader peptidase

lepA function, 83protein export, 83

lep operon proximal gene, 83leucine transport system

livH, 565leuK mutant

pseudouridine synthase I, 341levABCD, 1113lipoprotein antigen

cytoplasmic-membrane association,1072

identification, 1072immunochemical characterization,

1072purification, 1072

livH cloningleucine transport system, 565

lon mutantsdivision inhibition sensitivity, 756FtsZ overproduction, 756sulB mutations, 756

mal and pho regulon genesenvZ control, 706procaine treatment, 706

mal expressioneffect of osmolarity, 884maltose induction, 884

maltose-binding protein inhibitionsecA(Am) mutations, 505

maltose regulon osmoregulation, 884minicell expression system

livH, 565motB

nucleotide sequence, 244site-limited product incorporation, 244

new fla gene, 1007osmoregulationproP-mediated proline transport, 331

osmotic stress response, 253outer membrane

S. marcescens protease excretion,937

outer membrane Bacillus penicillinaseexcretionplasmid pMB9 kil gene, 728

pfkAexpression in Alcaligenes eutrophus,

319expression in gram-negative bacteria,

319pfkA gene expression

A. eutrophus, 319fructose use, 319gram-negative bacteria, 319

pho regulon mutantsalkaline phosphatase synthesis, 205nucleotide pool, 205

phototrophic mutationsphotoreversible pyrimidine dimers,

1141UV induction, 1141

polyamine-requiring mutantscompensatory polyamine formation,

128proline transport, 253pseudouridine synthase IleuK mutant, 341

rho gene transcriptionattenuation control, 945transcription termination factor rho,

945

ribosome degradationrole in cell death, 439

rifampin sensitivity, 824RNase H, 143RNase H-defectiveColEl plasmid replication, 361growth requirement, 361stable DNA replication, 361

secA(Am) synthesis defectmaltose-binding protein inhibition,

505pleiotropic effects, 505secretion-translation coupling, 505transcription regulation, 505

secA mutantschloramphenicol effects, 878growth suppression, 878protein secretion defects, 878temperature- and cold-sensitive muta-

tions, 878septationFtsA-PBP3 protein interaction, 985murein synthesis, 985

sex factor repression gene, 651sfrB gene

cloning and analysis, 651homology with S. typhimurium rfaH,

651Shiga-like toxin genes, 375SOS-associated division inhibition

reversibility, 733SfiA protein, 733

SOS regulatory systemcolicin D activity, 15

spfgene deletionspot 42 RNA function, 746

spot 42 RNA functionspf spot 42 RNA gene, 746

ssy mutationscold-sensitive growth, 849protein export defect suppressors, 849secY24 suppressors, 849

tnaL transcription termination, 217toxin conversion

bacteriophage H-19B, 375tryptophanase operon leader region

rho-dependent transcription termina-tion, 217

valinomycin-induced cation transport,334

verotoxin genes, 375EthionineB. subtilis sporulation, 1

Exfoliative toxin type BS. aureus, 574

ExopolysaccharidesP. syringae

alginate, 66levan, 66structure, 66

F1-ATPaseC. thermoaceticummacromolecular organization, 1128

Ferrienterobactin transportE. coli FepC genemembrane protein product, 930

F factor leading regionhomologous conjugative-plasmid

sequencesssb gene, 670

FhuA receptor proteinE. coli overproducing strain

VOL. 166, 1986

vi SUBJECT INDEX

cell envelopes, 405plasmid pHK232 programming, 405

FimbriaeB. nodosus

basal protein antigens, 453structural subunit, 453

Flagellar gene subdivisionS. typhimuriumassembly region, 187rotation region, 187switching region, 187

Flagellar rotationE. coliflaA locus

directional control, 1007Formate dehydrogenaseM. formicicum

molybdopterin cofactor, 500Frankia sp.

vesiclesisolation, 301nitrogen-fixing activity, 301

Fruiting body formationM. xanthus Dsp mutants, 842

Galactose permeaseS. cerevisiae GAL2, 313

,B-GalactosidaseB. stearothermophilus bgaB gene

flanking region, 722sequencing, 722

M. xanthus, 673GalactosyltransferaseR. meliloti

nodulation competitiveness, 148phage sensitivity, 148

Gene amplificationphage P1 Ap Cmhigh-copy-number replicons, 609ISI-dependent generation, 609

Genetic diversity and relationshipsBordetella spp.

metabolic enzyme electrophoreticvariation, 230

a-Glucans temperature-sensitive bindingB. stearothermophilus, 95

GlucoamylaseS. diastaticus gene expression

S. cerevisiae, 484S. pombe, 484

Glucose dehydrogenaseB. subtilis

developmental regulation, 238gene sequence and location, 238properties, 238sporulating-cell synthesis, 238

Glucose oxidaseP. chrysosporium, 269

Glucose uptakeSynechocystis sp.

fructose effects, 693Glutamine synthetase

E. coli mutantsammonium transport, 281

N. crassax and P polypeptides, 1040in vivo modification reaction, 1040oxidation inactivation, 1040

Glutamine synthetase adenylylationP. syringae toxin self-protection, 224

GlycerolH. influenzae transfection, 285

GlycoproteinM. xanthus vegetative cells, 644

Gram-negative bacteriaE. coli pfkA gene expression, 319

Gram-negative bacterial cell elongationkinetics"Lineola longa," 435

Haemophilus influenzaeplasmid transfer

effect of glycerol, 285transformasomes, 285

transfection, effect of glycerol, 285transformasomes

plasmid transfer, 285Halobacterium cutirubrum

13C nuclear magnetic resonance study,559

mevalonic acid synthesis, 559Halobacterium halobium

polyadenylated RNAisolation, 686molecular length, 686oligo(dT)-cellulose binding, 686

response oscillatorentrainment, 689temperature dependence, 689

Heat shock genesE. coli expression

heat shock regulatory protein, 380htpR gene product, 380

Heterokaryotic hyphaeC. neoformans genetic complementa-

tion, 924High-efficiency plasmid marker rescue

systemS. mutans genetic transformation, 658

Hydrogenase purificationR. japonicum, 795

Hydrogen peroxideE. coli, bimodal killing patterns

anoxically grown strains, 519DNA-repair-defective strains, 519

Hydroxamate biosynthesis by Yersiniaspp., 1131

HyphaeC. neoformans genetic complementa-

tion, 924

Indoleacetic acid-lysine synthetaseP. syringae iaaL gene

activity in E. coli, 598cloning, 598host-specific production, 598plant gall development, 598

Inositol metabolismS. pombe, 779

Insecticidal endotoxinB. thuringiensis genes

Diptera-specific product, 801Lepidoptera-specific product, 801

Invertase synthesisS. cerevisiae

catabolite repression mutants, 1123Iron transport

E. colienterobactin, 930fep genes, 930

Klebsiella pneumoniaeextracellular pullulanase

fatty acids, 1083lipoprotein, 1083

nifLA promoteroxygen sensitivity, 353

nitrogenase promoterdeletion analysis, 545nifA and ntrC product activation, 545

1-LactamaseB. licheniformis blaPmRNA features, 769transcriptional regulation, 769transcript synthesis, 769

Lactose utilizationS. aureus

phospho-p-galactosidase gene cloning,1061

Leader peptidaseE. coli lep and lepA, 83

Leucine transport systemE. coli livH gene, 565

Levansucrase productionB. subtilis

B. natto DNA fragment, 20Levansucrase regulation

B. subtilis sacB gene, 993"Lineola longa"

cell elongation kinetics, 435Lipase

S. aureus lysogenic conversiongeh lipase structural gene, 385phage L54a effect, 385

Lipid bilayer membraneB. pertussis

anion-selective porin, 212antibiotic uptake pathway, 212

Lipopolysaccharidenomenclature

historical overview, 699structure

nonenteric bacteria, 699smooth versus rough, 699

LipoproteinE. coli FhuA proteins

peptidoglycan association require-ment, 1137

K. pneumoniae extracellularpullulanase, 1083

Lipoprotein antigenE. coli

cytoplasmic-membrane association,1072

identification, 1072immunochemical characterization,

1072purification, 1072 poteichoic acids

Lipoteichoic acidscarbohydrate substitution, degree, 72critical micelle concentrations, 72

lon mutantsE. coli

division inhibition sensitivity, 756FtsZ overproduction, 756sulB mutations, 756

Macrolide-lincosamide-streptogramin BS. aureus resistance plasmid, 479

Maltose-binding proteinE. coli secA(Am), 505

Maltose regulonE. coli mal expression

effect of osmolarity, 884induction, 901

Membrane-particle-associated DNA se-quence

B. subtilis, 38Methanobacterium formicicum

J. BACTERIOL.

SUBJECT INDEX vii

formate dehydrogenasemolybdopterin cofactor, 500

Methanol dehydrogenaseMethylobacterium sp. structural gene,

581Methanol oxidation mutantsMethylobacterium sp.

complementation analysis, 581isolation, 581phenotypic characterization, 591

MethylasesE. coli dcm locus, 751

Methylobacterium sp.methanol dehydrogenase structural

gene, 581methanol oxidation mutant classes, 581,

591Methylophilus methylotrophus

electron transfer flavoproteincomposition and properties, 812trimethylamine dehydrogenase accep-

tor, 812Mevalonic acid synthesisH. cutirubrum

'3C nuclear magnetic resonancestudy, 559

Micelle concentrationslipoteichoic acids

effect of carbohydrate substitution, 72Mini-T plasmidsA. tumefaciens

plant cell transformation, 491T-DNA borders, activity, 491

Mollicutescomparison with E. coli, 824rifampin resistance, 824

Molybdopterin cofactorM. formicicum formate dehydrogenase

fluorescence, 500N. crassa nit-i complementation, 500pterin nucleus, 500

MotilityT. phagedenis periplasmic flagella, 105

Murein synthesisE. coli hipA, 399

Myxococcus xanthusDsp mutantsabnormal cell cohesion, 842developmental defect, 842social motility defect, 842

energy-dependent cell cohesioncorrelation with social motility, 837fruiting body formation, 842

frz genesgene product sizes, 673orientation, 673plasmid-encoded proteins, 673

[-galactosidase expression, 673vegetative cell surface glycoprotein

isolation, 644role in social behavior, 644surface structure, 644

NapthaleneP. putida

cellular association, 155uptake, 155

Neurospora crassaglutamine synthetasea and [3 polypeptides, 1040in vivo modification reaction, 1040oxidation inactivation, 1040

ornithine carbamoyltransferase

chloramphenicol effect, 679ethidium bromide effect, 679

NitrogenaseA. vinelandii structural gene mutants

nitrogenase activity, 180nitrogenase components, 180nitrogenase reconstitution, 180

C. pasteurianum genesbiased codon usage, 162nipH-like structure, 162

Nitrogenase iron proteinR. rubrum

ADP-ribosylation in vitro, 513Nitrogenase promoterK. pneumoniae

deletion analysis, 545nifA and ntrC product activation, 545

Nitrogen fixationBradyrhizobium sp. proton motive

force, 618Nitrogen-fixing activityFrankia vesicles, 301

N-Methyl-N'-nitro-N-nitrosoguanidinephage P22 mnt repressor gene mutation,

34NodulationR. japonicum

nodule development mutant, 628pseudonodule induction, 628soybean root nodule differentiation,

628Nodulation competitivenessR. meliloti galactosyltransferase, 148

Nodulation inhibitionR. leguminosarum nodABC genes, 552

Nomenclaturelipopolysaccharide

historical, 699structural, 699

Novel survival mechanismB. stearothermophilus, 95

ntrA-like mutantsA. vinelandii, 541

Ornithine carbamoyltransferaseN. crassa

chloramphenicol effect, 679ethidium bromide effect, 679

OsmoregulationE. coliproP-mediated proline transport, 331

Osmotic stress responseE. coli, 253

Oxygen responseE. coli cheB mutants, 275

Oxygen sensitivityK. pneumoniae nifLA promoter, 353

P1 plasmid prophagereplication controlgene amplification, 609high-copy-number replicons, 609ISl-dependent generation, 609

Periplasmic flagellaT. phagedenis

multiple protein species, 105role in motility, 105

Phanerochaete chrysosporiumglucose oxidase, 269ligninolytic cultures

glucose oxidase purification, 269Phaseolotoxin

P. syringae genes

clustering, 1096identification and cloning, 1096

Phosphate starvationS. typhimurium psiR loci, 666

Phospho-[-galactosidaseS. aureus gene

cloning in E. coli, 1061Phosphoenolpyruvate-dependent phos-

phorylationS. faecalisdihydroxyacetone and glycerol kinase

activity, 829enzyme I catalysis, 829HPr catalysis, 829

Phospholipid metabolismS. pombe, 779

Photoautotrophic mutantsSynechocystis sp., 693

Photoreversible pyrimidine dimersE. coli prototrophic mutationsUV light induction, 1141

Plant gall developmentP. iaaL gene

activity in E. coli, 598host-specific production, 598indoleacetic acid-lysine synthetase,

598Plant infectionR. leguminosarum

multicopy nodABC genes, 552nodulation inhibition, 552

PlasmidsBacteroides pBFTM1O and pB851

shuttle vector mobilization, 959ColD

colicin D activity, 15E. coli SOS regulatory system, 15mobilization, 15replication, 15

ColEl replicationRNase H, 361

C. taeniospiralis R-body-coding plas-mids, 349

DNA molecule partitioning defect, 100F factor leading regionhomologous conjugative-plasmid se-

quences, 670ssb gene, 670

H. influenzae transfection, 285IncFolac plasmid pED208

plasmid F product homology, 713plasmid R-100 product homology, 713surface exclusion genes, 713traS and traT nucleotide sequences,

713mini-F

E. coli ccd mechanism, 100mini-TA. tumefaciens plant cell transforma-

tion, 491T-DNA borders, activity, 491

novel high-efficiency marker rescue sys-temS. mutans genetic transformation, 658

pHK232E. coli FhuA production, 405

pMB9 kil geneE. coli penicillinase excretion, 728

P. putida CAM plasmidcam expression, 1089camR regulator, 1089

P. putida plasmid NAH7nahR gene product, 9

VOL. 166, 1986

viii SUBJECT INDEX

sal operon activation, 9P. putida plasmid pTN8

promoter structure, 739transcription-promoting DNA seg-

ment, 739pTiBo542A. tumefaciens supervirulence, 88map, 88virulence genes, 88

Rldrd-19 transfer genesco with conjugative plasmid F, 368fin genes, 368nucleotide sequences, 368tra genes, 368

S. aureusdeleted attenuator, 479macrolide-lincosamide-streptogramin

B resistance, 479Sym plasmidR. japonicum, 628

Ti plasmidAgrobacterium arginine catabolism,

44TOL plasmid-containing P. putida

cis-diol intermediates, 1028oxidation of aromatic acids, 1028

Polyadenylated RNAH. halobium

isolation, 686molecular length, 686oligo(dT)-cellulose binding, 686

Polyamine formationE. coli polyamine-requiring mutants,

128Polychlorinated biphenyl catabolism

P. pseudoalcaligenes bph genes, 392Procaine

E. coli mal-pho expression, 706Proline transport

E. coli, 253E. coli osmoregulation, 331

Protease expressionB. subtilis

B. natto DNA fragment, 20pleiotropic sacQ, 113

S. marcescens, 937Protein antigensB. nodosus fimbriae, 453

Protein synthesis elongation factors Tuand GA. nidulans genes, 78

Proton motive forceBradyrhizobium sp.K+ effects, 618membrane potential, 618nitrogen fixation effects, 618oxygen tension effects, 618

Pseudomonas aeruginosaphage D3

IS222 identification, 1134lipopolysaccharide structural changes,

1134Pseudomonas pseudoalcaligenes

biphenyl degradation genes, 392 bphgenes, 392

chlorobiphenyl degradation genes, 392Pseudomonas putidacamR regulatorcytochrome P-450c,, hydroxylase

operon, 1089NAH7 plasmidnahR, 9sal, 9

naphthalenecellular association, 155uptake, 155

naphthaline metabolism, 9oxidation of aromatic acids

cis-diol intermediates, 1028TOL plasmid-encoded enzymes, 1028

pTN8 promoter structure, 739salicylate metabolism, 9sal operon, 9transcription-promoting DNA segment,

739Pseudomonas syringae

exopolysaccharidesalginate, 66levan, 66structural characterization, 66

glutamine synthetase adenylylationself-protection from tabtoxinine-p-

lactam, 224phaseolotoxin genes

clustering, 1096identification and cloning, 1096

toxin self-protectionglutamine synthetase adenylylation,

role, 224Pseudomonas syringae subsp. savastanoi

indoleacetic acid-lysine synthetaseE. coli, activity, 598host-specific production, 598iaaL gene cloning, 598plant gall development, 598

Pseudouridine synthase IE. coli leuK mutant, 341

PullulanaseK. pneumoniae

fatty acids, 1083lipoprotein, 1083

Pyrogenic exotoxin Astreptococcal productionphage T12, 623speA gene, 623

RAS genesfunction in mammalian cells, 364S. cerevisiae growth control, 364

Response oscillatorH. halobium, 689

Rhizobium japonicumhydrogenase

aerobic purification, 795affinity chromatography, 795

soybean root nodule differentiationlocus affecting development, 628

transposon insertion nodule develop-ment mutantpseudonodule induction, 628Sym plasmid, 628

Rhizobium leguminosarumnodulation inhibition

multicopy nodABC genes, 552plant infection, early stages, 552

plant infection, early stages, 552Rhizobium meliloti

galactosyltransferasenodulation competitiveness, 148phage sensitivity, 148

Rhodospirillum rubrumchromatophore membrane composition

cell cycle-specific oscillation, 818protein/bacteriochlorophyll a ratio,

818protein/phospholipid ratio, 818

nitrogenase iron proteinADP-ribosylation, 513reversible regulation, 513

Rho transcription termination factorE. coli, T35

Ribosome degradationE. coli cell death, 439

RNA polymeraseB. subtilis, 472mollicutescomparison with E. coli, 824rifampin insensitivity, 824

S. typhimuriumincreased UTP pools, 857pyr hyperexpression, 857reduced chain elongation rate, 857

RNase HE. coli, 143

Saccharomyces cerevisiaeadenosine accumulation

effect of adenine levels, 763adenosine deamination, 763catabolite repression of invertase

cyc8 genes, 1123tupi genes, 1123

choline transport gene, 328GAL2membrane-bound galactose permease

subunit, 313galactose permeaseGAL2-lacZ fusions, 313plasma membrane export and assem-

bly, 313temperature-conditional secretion mu-

tants, 313invertase synthesis-constitutive

mutants, 1123RAD2 gene

effect of UV irradiation, 914nucleotide sequence, 914regulation, 914transcript mapping, 914

RAS genesfunction in mammalian cells, 364growth control, 364

ungi mutants, 905uracil-DNA-glycosylase-defective mu-

tantsselection by genetic transformation,

905Saccharomyces diastaticusglucoamylase gene expression

S. cerevisiae, 484S. pombe, 484

Salmonella typhimuriumDNA sequencesA + T content conservation, 1113

flagellar gene subdivisionassembly region, 187rotation region, 187switching region, 187

levABCD, 1113miaAl mutation, 1013phosphate starvation regulon, 666psi loci, identification

phage Mu, 666pyr hyperexpressionRNA polymerase association, 857

rfaHhomology with E. coli sfrB gene, 651

RNA polymerasepyr gene control, 857

J. BACTERIOL.

SUBJECT INDEX ix

reduced chain elongation rate, 857rpoBC mutant

increased UTP pools, 857tRNA mnodification-defective mutants

effect of increased salt, 1013pleiotropic cell physiology effects,

1013Schizosaccharomyces pombe

inositol metabolism, 779phospholipid metabolism, 779

SeptationE. coli FtsA protein, 985

Serratia marcescensmetalloprotease, 937protease excretion

E. coli outer membrane, 937Sex factor repression gene

E. coli sfrB, 651Shuttle vectors

E. coli-Bacteroides spp.mobilization by Bacteroides Tcr ele-

ment, 959mobilization by IncP plasmids, 959

S-layerT. tenax and T. hzeutrophilus

gross morphology, 1046morphogenetic function, 1046

SLP1 elementS. coelicolorautonomous replication, 999excision and integration, 999reversible site-specific recombination,

999.social motility of cells

M. xanthuscell agglutination, 837cell cohesion, 837

SOS-associated division inhibitionE. coli

reversibility, 733SfiA protein, 733

Spiroplasma DNA-dependent RNA poly-merasecomparison with E. coli sensitivity, 824insensitivity to rifampin, 824

Spore proteinB. subtilis ssp genes, 412, 417

SporulationB. subtilisDNA-dependent RNA polymnerase,

induction, 472ethA, 1ethionine induction, 1glucose dehydrogenase synthesis, 238in vitro- and in vivo-transcribed

genes, 472stage 0 mutations, 173subtilisin expression, 173

Spot 42 RNA functionE. coli spf gene, 746

Staphylococcal lipasephage DNA inactivation, 385

Staphylococcus aureuscadmium resistance gene(s), 574entB nucleotide sequence, 29enterotoxin B gene

nucleotide sequence, 29exfoliative toxin type B gene, 574lipase lysogenic conversiongeh lipase structural gene, 385phage L54a effect, 385

macrolide-lincosamide-streptogramin Bresistance, 479

phospho-3-galactosidase genecloning in E. coli, 1061lactose utilization, 1061

pRWO01 structural genesbacteriocin immunity, 574bacteriocin production, 574cadmium resistance, 574Cd(NO3)2 resistance, 574exfoliative toxin B production, 574

Starch bindingB. stearothermophilus, 95

Streptococcipyrogenic exotoxin A production

bacteriophage T12 involvement, 623Streptococcus faecalis

dihydroxyacetone and glycerol kinaseactivityenzyme I catalysis, 829HPr catalysis, 829phosphoenolpyruvate-dependent

phosphorylation, 829mnutans

genetic transformationhigh-efficiency plasmid marker rescue

system, 658sucrose phosphotransferase system

scrB cloning and characterization, 426sucrose-6-phosphate hydrolase, 426

Streptococcus pneumoniaeQl(cat-tet), 978chromosomal conjugative elements

cat-tet element, 972, 978cloning and characterization, 972

conjugative element, structure, 978E. coli recombinant plasmids, 972

Streptomyces coelicolorSLP1 elementautonomous replication, 999excision and integration, 999reversible site-specific recombination,

999Streptomyces erythreus

bacteriophages, characterization, 1055phage-resistant mutants, 1055

Succinate dehydrogenaseB. subtilis sdh gene

cytochrome b558, 1067nucleotide sequencing, 1067

Sucrose-6-phosphate hydrolaseS. mutans, 426

Sucrose phosphotransferase systemS. mutans scrB, 426

Sulfur-oxidizing abilityT. pantotropha

effects of TnS-induced mutations, 446Superoxide dismutasesB. thetaiotaomicron

isolation and reconstitution, 528Suppressor tRNAscodon context sensitivity, 1022translational suppression and accuracy,

1022Surface exclusion genes

IncFolac plasmid pED208homology with plasmids R-100 and F,

713sfx system, 713traS and traT nucleotide sequences,

713Synechocystis sp.

fructose-glucose pernlease interaction,693

glucose uptake, 693

photoautotrophic mutants, 693

Tabtoxinine-p-lactamP. syringae

glutamine synthetase adenylylation,224

self-protection, 224Temperature sensitivity

E. cloacae nifA, 357Thermoproteus spp.

cell envelope ultrastructurelattice orientation, 1046paracrystalline arrays,. 1046S-layer gross morphologies, 1046S-layer shape-determining function,

1046S-layer

gross morphology, 1046morphogenetic function, 1046

Thermus thermophilusgenetic transformation, 338

Thiosphaera pantotrophachemolithoautotrophic-growth mutants,

446sulfur-oxidizing abilityTnS-induced mutations, effects, 446

Transposonlike elementsC. taeniospiralis, 349

Treponema phagedenisperiplastnic flagella

multiple protein species, association,105

role in motility, 105tRNA

B. subtilis, genesexpression in E. coli, 306

translational suppression and acturacycodon context sensitivity, 1022modification next to the anticodon,

1022Tryptophanase

E. coli tnaL transcription termination,217

Tryptophan operon expression regdilationB. subtilis, 461

Tunicamycin resistanceB. subtilis amyE-tmrB region, 787

Uracil-DNA-glycosylase-deficient mutantsS. cerevisiae

mitochondrial uracil-DNA-glycosylaseactivity, 905

nuclear uracil-DNA-glycosylase activ-ity, 905

ungi, 905UV irradiation

S. cerevisiae RAD2 genesteady-state mRNA levels, 914

UV lightE. coli lexA51 mutants

photoreversible pyrimidine dimers,1141

phototrophic mutations, 1141

VesiclesFrankia sp.

isolation, 301nitrogen-fixing activity, 301

Vitamin B12B. megaterium 51

Yersinia spp.aerobactin production, 1131hydroxamate biosynthesis, 1131

VOL. 166, 1986

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