Isolation and Characterization of E. coli from Day Old Chicks of a Selected Breeder Farm in Bangladesh

There are 3867000 breeders in 227 hatcheries in Bangladesh1

Bacterial diseases caused highest mortality in chicken2

Very few attempts were taken to study the E. coli of day old

chick( DOC)3,4

E. coli of DOC is important both for DOC and the parent stock.

Objectives

Study design: cross-sectional

Duration: April 2008 to May 2009

Study site: laboratory of the Department of Microbiology and Hygiene,

Bangladesh Agricultural University,Mymensingh

Visceral sample collected aseptically from eighteen DOC of different

groups were collected from a Breeder farm of Valuka, Mymensingh

Tested the incubated sample for E.coli through competitive cultural

examination and biochemical tests

Total Viable Count (TVC) was done on nutrient agar following ten-fold

dilution

Lastly antibiotic sensitivity test was conducted to know their drug

sensitivity and resistance pattern.

1. Anonym, 2010. Farm Poultry And Livestock Survey. Bangladesh

Bureau of Statistics, Statistics Division, Ministry of Planning,

Government of the People’s Republic of Bangladesh.

2. Uddin MZ, Samad MA , Kabir SML, 2011. Mortality and disease

status in hy-line and isa-brown strains of layer chickens reared in cage

system in Bangladesh. Bangl J Vet Med, 9, 1 –16.

3. Islam A, Majumder S, Rahman A, Trisha AA, Amin R, 2012.

Retrospective study of commercial poultry diseases. Eurasian J. Vet.

Sci. 28, 2, 116-121.

4. Islam A, Trisha AA, Das M, Amin MR, 2009. Retrospective study of

some poultry diseases at Guibandha district in Bangladesh. Bangl J Vet

Med, 7, 239-247.

M.A. Rashid1, 2, K.A. Chowdhury1, S.M.S. Mahmud1, T. Chowdhury3, Abdulla Al Mamun Bhuyan4, Zannat – Nahar1, S.K.Paul1,3*

 

Background Results

Conclusions and Recommendations

References

• From the findings of different cultural, staining, biochemical

examinations it may be concluded that the isolated organism was E. coli

The load of E. coli was higher among the lower grade chicks and in gut

The E. coli we isolated during this study showed resistance to certain

antibiotics which tends to recommend the proper use of antibiotics

In future more extensive studies can be conducted to design a

successful control estrategias

We were able to isolate E. coli was from gut, liver and lungs of DOC

(Table 1).

Total viable count (TVC) in gut of “A” grade chicks was 2.3×106, in “B”

grade 2.6×106, in “C” grade 2.9×106, mean of which was 2.6×106

(Table 2).

The TVC of liver in “A” grade chicks was 2.6×105, in “B” grade

2.7×105, in “C” grade 2.8×105, the mean of which was 2.6×105 (Table

2).

in the lungs of “A” grade chicks was 2.2×104, in “B” grade 2.3×104 and

in “C” grade was 2.5×104. The mean of which was 2.3×104 (Table 2).

Antibiotic sensitivity test revealed that E. coli were sensitive to

Ciprofloxacin, Amoxicillin and Enrofloxacin, but resistant to

Erythromycin and Cloxacillin.

Methods

To determine the E.coli in vital organs like liver, lungs and gut of

healthy (A grade), weak (B grade), culled (C grade) chicks obtained

immediately after hatching

To determine the Total Viable Count of bacteria to show the

difference in their density in different organs of the three grade of

chicks

To determine the antibiotic sensitivity pattern of isolates

Acknowledgements I am grateful to Department of Microbiology and Hygiene, Faculty of

Veterinary Science, Bangladesh Agricultural University ,Mymensingh

-2202, Bangladesh. Special thanks to Dr.Najmul haider ,Dr.Ausraful

islam and Dr. Sukanta (RI of icddr,b) for their valuable suggestion to

preparing my poster.

1Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh2Department of Livestock Services,3International Centre for Diarrheal Disease Research (icddr,b), Dhaka, Bangladesh,4Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Bangladesh

A” quality chicks (Grade-A)

B” quality chicks (Grade-B)

C” quality chicks (Grade- c)

Correspondence: paul@icddrb.org

Batch No. Grade No of chicks Sample type E.Coli present

Batch 1

A 2 Gut Present

B 2 Gut Present

C 2 GutLiverLung

Present

Batch 2

A 2 Gut Present

B 2 GutLiver

Present

C 2 GutLiverLung

Present

Batch 3

A 2 Gut Present

B 2 GutLiver

Present

C 2 GutLiverLung

Present

Table 1. Bacteria isolated from different organs of 3 batches of DOC

Table 2. Total viable count (TVC) obtained from gut of DOC

Sample type Grade TVC (CFU/ml) Mean ±SD

Gut

A 2.3×106

2.6×106

B 2.6×106

C 2.9×106

Liver

A 2.6×105

2.7×105

B 2.7×105

C 2.8×105

Lung

A 2.2×104

2.3×104

B 2.3×104

C 2.5×104

Test Result

Reaction with Dextrose

Maltose

Lactose

Sucrose

Mannitol

All are +

Indole +

MR +

VP -

Table 3. Biochemical characteristics of E. coli

+ indicates Positive, MR = Methyl Red, - indicates Negative, VP= Voges-Proskauer

E. coli growth on EMB agar media-Metallic shin

E. coli growth on SS agar

E.Coli growth on Blood agar

Antibiotic sensitivity pattern

Total viable count (TVC) of E. coli

CIP

AMOXENRO