VOLUME 54^

1986

INTERNATIONALJOURNAL OF LEPROSYAnd Other Mycobacterial Diseases

Official Organ of theINTERNATIONAL LEPROSY ASSOCIATION(Societe Internationale de la Lepre)

(AsociaciOn Internacional contra la Lepra)

Contents

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

Official Organ of the International Leprosy Association

President: Prof. M. F. Lechat, Ecole de Sante Publique-U.C.L.,Epid. 30.34, Clos Chapelle aux Champs 30,1200 Bruxelles, Belgium

Secretary: Dr. R. H. Thangaraj, The Leprosy Mission,CN I Bhawan, 16 Pandit, Pant Marg,New Delhi 110001, India

Treasurer: Dr. W. F. Ross, One Broadway,Elmwood Park, New Jersey 07407, U.S.A.

EDITORIAL OFFICEGillis W. Long Hansen's Disease Center

Carville, Louisiana 70721, U.S.A.

Editor: ROBERT C. HASTINGS, Carville, Louisiana

Assistant Editor: DEE D. GOODMAN, Carville, Louisiana

BUSINESS AND CIRCULATION OFFICEExecutive Officer: W. FELTON Ross

One BroadwayElmwood Park, New Jersey 07407, U.S.A.

BOARD OF DIRECTORSDr. Michel F. Lechat (President, ILA), ChairmanDr. Patrick J. BrennanDr. Wayne M. MeyersDr. W. Felton Ross (Treasurer, ILA)Dr. R. H. Thangaraj (Secretary, ILA)

CONTRIBUTING EDITORSArgentina: Dr. J. E. Cardama

(Buenos Aires)Australia: Dr. J. C. Hargrave (Darwin)Brazil: Dr. L. M. Bechelli (Sao Paulo)France & Fr. Terr.: Dr. D. Wallach

(Paris)India (North): Dr. L. K. Bhutani

(New Delhi)India (South): Dr. M. Christian

(Karigiri)Israel: Dr. L. Levy (Jerusalem)Japan: Dr. M. Abe (Tokyo)Malaysia & SE Asia: Dr. J. H. S.

Pettit (Kuala Lumpur)

Mexico: Dr. A. Saul (Mexico, D.F.)Netherlands: Dr. D. L. Leiker (Amsterdam)People's Republic of China:

Dr. Ye Gan-yun (Taizhou)Philippines: Dr. R. M. Abalos (Cebu City)Spain: Dr. J. Terencio de las Aguas (Fontilles)U.K.: Dr. R. J. W. Rees (London)U.K.: Dr. M. F. R. Waters (London)U.S.A.: Dr. P. W. Brand (Carville)U.S.A.: Dr. Thomas H. Rea

(Los Angeles)U.S.S.R.: Dr. A. A. Juscenko (Astrakhan)Venezuela: Dr. J. Convit (Caracas)West Africa: Dr. M. Nebout (Bamako)

This JOURNAL IS indexed in CURRENT CONTENTS/LIFE SCIENCES, BIOLOGICAL ABSTRACTS,and CHEMICAL ABSTRACTS. Microform available from University Microfilms

International, Ann Arbor, Michigan 48106, U.S.A. or London WC1R 4EJ, U.K.

The INTERNATIONAL JOURNAL OF LEPROSY (ISSN 0148-916X) is published quarterly for $60.00 peryear for members and $80.00 per year for subscribers by Allen Press, Inc., 1041 New Hampshire St.,Lawrence, Kansas 66044, U.S.A. Second-class postage paid at Elmwood Park, New Jersey and ad-ditional mailing office. POSTMASTER: Send address changes to INTERNATIONAL JOURNAI. OF LEP-ROSY, Business and Circulation Office, One Broadway, Elmwood Park, NJ 07407, U.S.A.

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

CONTENTSVolume 54, Number 1, March 1986

Original Articles^ PageKvach, James T., Neubert, Thomas A., Palomino, Juan Carlos, and Heine, Henry S.

Adenosine Triphosphate Content of Mycobacterium leprae Isolated from Armadillo Tissueby Percoll Buoyant Density Centrifugation. ^1

Shepard, Charles C., Rees, Richard J. W., Levy, Louis, Pattyn, Stefaan R., Ji Baohong,and Dela Cruz, Eduardo C. Susceptibility of Strains of Mycobacterium leprae IsolatedPrior to 1977 from Patients with Previously Untreated Lepromatous Leprosy. ^11

Almeida, Joel Gerald, Jesudasan, Kumar, Christian, Melville, and Chacko, Chinoy JohnGeorge. Relapse Rates in Lepromatous Leprosy According to Treatment Regularity. ^16

Huikeshoven, Han and Madarang, Monina G. Spot Test for Detection of Dapsone in Urine:An Assessment of Its Validity and Interpretation in Monitoring Dapsone Self-administra-tion. ^21

Ponnighaus, Jorg M. and Fine, Paul E. M. Sensitization Studies with Potential LeprosyVaccine Preparations in Northern Malawi. ^25

Garraud, Olivier, Ribierre, Olivier, and Bach, Marie -Anne. A Follow up of T-cell Subsetsand of Anti-M. leprae Antibody Titer as Measured by the FLA-ABS Test in MelanesianLeprosy Patients Under Polychemotherapy. ^38

Narayanan, R. B., Ramu, G., Malaviya, G. N., Sinha, S., and Sengupta, U. Immunohis-tological Analysis of Skin Reaction to My1 Derived from Mycobacterium leprae. ^46

Balybin, Eugene Sergeevich and Naumov, Valentine Zaharovitch. Dependence of Pro-liferative Activity of Lymphocytes on Basal Levels of Lymphocytic Cyclic Nucleotides inLepromatous Leprosy Patients. ^52

Chirmule, Narendra B., Deo Madhav G., Sirodkar, Manohar V., Deshmukh, Ranjana, andChanderkar, Narendra G. ICRC Vaccine-induced Changes in M. leprae-specific Cell-mediated Immunity in Langur (Presbytis entellus) Monkeys. ^57

Douglas -Jones, Anthony G., Wade, Shona, Kent, David, Vaughan, Ross, and Watson,James D. Immunity to Leprosy. III. The in vitro Induction of B Lymphocyte Proliferation byMycobacteria. ^63

Band, Abdul Hamid, Bhattacharya, Alok, and Talwar, Gursaran, P. Lack of Mycobac-terium /eprae-specific Uptake in Schwann Cells. ^71

Chandi, Sushi! M. and Chacko, Chinoy J. G. An Ultrastructural Study of the Response ofTraumatized Rabbit Tibial Nerve to Epineurial Infection with Mycobacterium leprae. ^79

Harada, Kiyoshi and Suzuki, Keiji. Periodic Acid-ethanol Gelatin Met henamine Silver Stainfor Demonstrating M. leprae and Myelin in Peripheral Nerve Fibers of Leprosy Patients. ^84

EditorialThe 1985 JOURNAL—a Continuing Perspective. (Robert C. Hastings) ^88

ObituariesCochrane, Robert Greenhill ^109Gass, Herbert H. ^120

CorrespondenceSulfono-resistance de Mycobacterium /eprae—Monotherapie par la Diaminodiphenyl-

sulfone—Interet des Associations Mêdicamenteuses Triples. (Hervê A. Floch) ^122Erythema Nodosum Leprosum Associated with Azotemic Acute Glomerulonephritis

and Recurrent Hematuria. (Robert H. Gelber) ^125Epithelioma Cuniculatum in Leprotic Foot. (W. M. Ramadan, A. El-Aiat and M. H ^

Hassan) ^127A More Malignant Course of Leprosy Infection in Armadillos After Inoculation with

Sonicated Suspension of Mycobacterium leprae. (J. F. Kazda) ^129Sudden Respiratory Collapse in an Armadillo (Dasypus novemcinctus, Linn.). (Ian A ^

Cree, William Guthrie, A. Colin McDougall and R. J. W. Rees) ^131Xenograft Studies in Leprous Neuropathy. (Vanaja P. Shetty and Verghese George) ^133In vitro Assessment of Endothelial Cell Response to Mycobacterium leprae. (Nader

M. Damavandi and Lata Mehta) ^135Clofazimine-resistant M. leprae. (Louis Levy) ^137

News and Notes ^141Book Reviews ^147

Current Literature ^149Special Grantors and Sustaining Members ^165Index, Volume 53 ^166

CONTENTSVolume 54, Number 2, June 1986

Original Articles^ PageStanley, John N. A., Pearson, John M. H., and Ridley, Dennis B. Episodic Edema in Type

2 Lepra Reaction Can Be Caused by Transient Lymphatic Obstruction in the Lymph Node.231

Groenen, Guido, Janssens, Luc, Kayembe, Tshilumba, NoIlet, Erik, Coussens, Luk, andPattyn, Stefaan R. Prospective Study on the Relationship Between Intensive BactericidalTherapy and Leprosy Reactions. 236

Papaioannou, Dimitri J., Kaklamani, Evangelia P., Parissis, Nikos G., Koumantaki, !von'G., Karalis, Dimitri Th., and Trichopoulos, Dimitri B. Hepatitis B Virus (HBV) SerumMarkers in Greek Leprosy Patients. 245

Rathi, S. S., Chaturvedi, V. N., Raizada, R. M., and Jain, S. K. T. Electrogustometry inHansen's Disease (Study of 225 Cases). ^252

Bach, Marie -Anne, Wallach, Daniel, Flageul, Beatrice, Hoffenbach, Agnes, and Cottenot,Francois. Antibodies to Phenolic Glycolipid-1 and to Whole Mycobacterium leprae inLeprosy Patients: Evolution During Therapy. ^256

Narayanan, R. B., Girdhar, Bhawneshwar K., Lavania, Ravinder K., and Sengupta, Utpal In vitro Studies on Dermal Granulomas of Human Leprosy: Characterization of Cells UsingMonoclonal Antibodies. 268

Gelber, Robert H., Humphres, Robert C., and Fieldsteel, A. Howard. Superiority of theNeonatally Thymectomized Lewis Rat (NTLR) to Monitor a Clinical Trial in LepromatousLeprosy of the Two Regimens of Rifampin and Dapsone. 273

Kohsaka, Kenji, Miyata, Yasuyo, Mori, Tatsuo, and Ito, Tonetaro. Prophylactic/Thera-peutic Effects and Induction of Reversal Reactions with Thymus Transplantation in Myco-bacterium /eprae-infected Nude Mice. 284

Harris, Eugene B., Li, Yu -Teh, and Li, Su -Chen. Ganglioside Patterns in Normal and Lep-romatous Armadillo Tissues. ^289

Band, Abdul Hamid, Chitamber, Shobha D., Bhattacharya, Alok, and Talwar, GursaranP. Mechanism of Phagocytosis of Mycobacteria by Schwann Cells and Their Comparisonwith Macrophages. 294

EditorialLeprosy and Social Class in the Middle Ages. (Stephen R. Ell) ^300

ObituariesBrowne, Stanley G. ^306Nakayama, Tetsu ^308

CorrespondenceLysis of M. leprae by Macrophages in vitro. (Bernardo Beiguelman) ^309A Multifactorial Culture Medium with Growth Factors from Leprosy-derived Mycobac-

teria Proposed in Cultivation Trials for Mycobacterium leprae. (Laszlo Kato) ^310News and Notes ^312Book Reviews ^322Current Literature ^324Special Grantors and Sustaining Members ^365

CONTENTSVolume 54, Number 3, September 1986

Original Articles^ PageDouglas-Jones, Anthony G., Wade, Shona M., Vaughan, Ross H., and Watson, James

D. Immunity to Leprosy. IV. Murine T-Cell Proliferative Responses to Mycobacteria. ^367

Dedhia, Narendra M., Almeida, Alan F., Khanna, Umesh B., Mittal, Bharati V., and Achar-ya, Vidya M. Acute Renal Failure—A Complication of New Multidrug Regimen for Treatmentof Leprosy. 380

Pieters, Frans A. J. M., Zuidema, Jan, and Merkus, Frans W. H. M. Sustained ReleaseProperties of an Intra-adiposely Administered Dapsone Depot Injection. ^383

Kar, H. K., Bhatia, V. N., Harikrishnan, S. Combined Clofazimine- and Dapsone-resistantLeprosy. A Case Report. ^389

Levine, Seymour and Saltzman, Arthur. Clofazimine Enteropathy: Possible Relation to Pey-er's Patches. ^392

Segasothy, Murugasu, Muhaya, Haji Mohamed, Musa, Ashaari, Rajagopalan, Krishnan,Lim, Kuan Joo, Fatimah, Yakin, Kamal, Ahmad, and Syed Ahmad, Kabeer. AnalgesicUse by Leprosy Patients. 399

Odinsen, Odd, Nilson, Tomas, and Humber, David P. Viability of Mycobacterium leprae:A Comparison of Morphological Index and Fluorescent Staining Techniques in Slit-skin Smearsand M. leprae Suspensions. 403

Katoch, Vishwa M. and Cox, Robert A. Step-wise Isolation of RNA and DNA from Myco-bacteria. ^409

Khandekar, Pramod S., Munshi, Anil, Sinha, Subrata, Sharma, Geeta, Kapoor, Archana,Gaur, Amitabh, and Talwar, G. P. Construction of Genomic Libraries of MycobacterialOrigin: Identification of Recombinants Encoding Mycobacterial-specific Proteins. 416

Narayanan, R. B., Girdhar, Bhawneshwar K., and Desikan, K. V. OKT6+ Epidermal Lan-gerhans Cell Numbers in DNCB Reactions Among Leprosy Patients. ^423

Baskin, Gary B., Gormus, Bobby J., Martin, Louis N., Wolf, Robert H., Watson, EdwinA., Walsh, Gerald P., Meyers, Wayne M., and Binford, Chapman H. The LeprominTest in Rhesus Monkeys. 427

Jeevan, Amminikutty, Bapat, C. V., and Deo, M. G. Adoptive Transfer of Tolerance Inducedby ICRC Bacilli Against Mycobacterium leprae in Mice. ^437

^Nomaguchi, Hiroko, Kohsaka, Kenji, Miyata, Yasoyo, Mori, Tatsuo, and Ito, Tonetaro ^Restored Pathogenicity of Attenuated Mycobacterium lepraemurium in Mice. ^446

Job, Charles K., Harris, Eugene B., Allen, Joe L., and Hastings, Robert C. A RandomSurvey of Leprosy in Wild Nine-banded Armadillos in Louisiana. ^453

EditorialMycobacterium avium-complex Infections and Development of the Acquired Immuno-

deficiency Syndrome: Casual Opportunist or Causal Cofactor? (Frank M. Collins) ^458

CorrespondencePurification of M. leprae Isolated from Human Skin Biopsies. (Sen - Chiew Gan) ^475Enzyme Activation in Peritoneal Cells from Mice Infected with Mycobacterium leprae-

murium. (Oscar Rojas - Espinosa, Antonina Oltra, Eira Ouevedo, and Patrice Arce) ^476^Lepromin Skin Test in Normal People in Singapore—A One -year Follow Up. (K. V ^

Ratnam) ^479^Bilateral Ulnar Nerve Abscess in Lepromatous Leprosy; A First Encounter. (Robert H ^

Gelber and Alan G. Zacharia) ^480

News and Notes ^483Current Literature ^487Statement of Financial Condition ^498Special Grantors and Sustaining Members ^502

CONTENTSVolume 54, Number 4, December 1986

Original Articles^ Page

^Converse, Paul J. and Bjune, Gunnar. Natural Killer (NK) Cell Activity and Reversal Reaction in Leprosy.^503Pieters, Frans A. J. M. and Zuidema, Jan. lntra-adipose Administration of Monoacetyldapsone to Healthy

Volunteers. ^510Fischer, James H., West, Dennis P., and Worobec, Sophie M. Evaluation of a Continual Compliance

Monitoring Program for Dapsone in an Outpatient Hansen's Disease Clinic. ^517Jain, Ganpat Lal, Pasricha, Jagjit Singh, and Guha, Sujoy Kumar. Objective Grading of the Loss of

Pain and Touch Sensations in Leprosy Patients. ^525

Meeker, Harry C., Levis, William R., Sersen, Eugene, Schuller-Levis, Georgia, Brennan, Patrick J.,and Buchanan, Thomas M. ELISA Detection of IgM Antibodies Against Phenolic Glycolipid-I in theManagement of Leprosy: A Comparison Between Laboratories. ^530

Kumar, Bhushan and Kaur, Surrinder. Selection of Sites for Slit Skin Smears in Untreated and TreatedLeprosy Patients. ^540

Britton, Warwick J., Hellqvist, Linus, and Basten, Antony. Separate Antigenic Determinants on Cell WallAssociated Carbohydrate Antigens of Mycobacterium leprae Defined with Monoclonal Antibodies ^545

Truman, Richard W., Morales, Melvyn J., Shannon, Edward J., and Hastings, Robert C. Evaluationof Monitoring Antibodies to PGL-I in Armadillos Experimentally Infected with M. leprae. ^556

Saito, Hajime, Tomioka, Haruaki, and Nagashima, Kiyofumi. In Vitro and in Vivo Activities of OfloxacinAgainst Mycobacterium leprae Infection Induc‘ i in Mice. ^560

Ji Baohong, Chen Jiakun, Lu Xizh—i. Wang ■yu, Ni Guoxing, Hou Yuhong, Zhou Daohai, and TangQuankui. Antimycobactenal Activities of Two Newer Ansamycins, R-76-1 and DL 473. ^563

Lloyd, Geoffrey M. and Draper, Philip. Separation of Mycobacterium leprae from Contamination withArmadillo-liver-derived "Pigment" Particles. ^578

Mori, Tatsuo and Kohsaka, Kenji. Identification of Cat Leprosy Bacillus Grown in Mice. ^584Ridley, Dennis S. and Ridley, Marian J. Classification of Nerves is Modified by the Delayed Recognition

of Mycobacterium leprae. ^596Cree, Ian A., Gardiner, Carol A., Beck, J. Swanson, and Mehta, Jal. Studies of Cell Death (Apoptosis)

and Cell Division in Leprosy Granulomas. ^607Fine, Paul E. M., Job, Charles K., McDougall, A. Colin, Meyers, Wayne M., and Ponnighaus, Jorg M

Comparability Among Histopathologists in the Diagnosis and Classification of Lesions Suspected of Leprosyin Malawi. 614

Reprinted ArticleGrange, J. M. and Yates, M. D. Infections Caused by Opportunistic Mycobacteria: A Review. ^626

EditorialHost-Parasite Interrelationship Between M. leprae and Schwann Cells in Vitro. (R. Mukherjee and N.

H. Antia) ^632

ObituaryHuikeshoven, Han

^639

CorrespondenceMacrophage-induced Procoagulation Assay for the Detection of Defective Macrophage Activation

in Leprosy Patients. (Hoang Thuy Long, Pham Manh Hung, and E. P. Wright) ^641Trial of Cimetidine in Lepromatous Leprosy—A Futile Attempt. (Narender K. Mathur, Ram A. Bumb,

Dinesh C. Mathur, and Suresh K. Jain) ^644Leprosy in Children One Year of Age and Under. (M. E. Duncan) ^646Leprosy and Social Class in the Middle Ages. (Harry L. Arnold, Jr.) ^647Dr. Ell's Response. (Stephen R. Ell) ^647Shakespeare's "Hoar Leprosy." (Abrando Rotberg) ^648The "Ex Libris" of Dr. N. A. Torshujev. (Laszlo Kato) ^649Osier on Leprosy. (Anand Date) ^651

News and Notes ^654

Book Reviews ^664

Current Literature ^671

U.S.—Japan Cooperative Medical Science Program, Twenty-First Joint LeprosyResearch Conference, Osaka, Japan, 1986 ^712

Reviewers ^737

International Leprosy Association Organization and Members ^738

Special Grantors and Sustaining Members ^751

Statement of Ownership ^752