VOLUME 53^

1985

INTERNATIONALJOURNAL OF LEPROSYAnd Other Mycobacterial Diseases

Official Organ of theINTERNATIONAL LEPROSY ASSOCIATION(Societe Internationale de la Lêpre)

(Asociacieln Internacional contra la Lepra)

Contents

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

Official Organ of the International Leprosy Association

President: Prof. M. F. Lechat, Ecole de Sant& Publique-U.C.L.,Epid. 30.34, Clos Chapelle aux Champs 30,1200 Bruxelles, Belgium

Secretary: Dr. R. H. Thangaraj, The Leprosy Mission,CN1 Bhawan, 16 Pandit, Pant Marg, New Delhi 110001,India

Treasurer: Dr. W. F. Ross, One Broadway,Elmwood Park, New Jersey 07407, U.S.A.

EDITORIAL OFFICENational Hansen's Disease CenterCarville, Louisiana 70721, U.S.A.

Editor: ROBERT C. HASTINGS, Carville, Louisiana

Assistant Editor: DEE D. GOODMAN, Carville, Louisiana

BUSINESS AND CIRCULATION OFFICEExecutive Officer: W. FELTON Ross

One BroadwayElmwood Park, New Jersey 07407, U.S.A.

BOARD OF DIRECTORSDr. Michel F. Lechat (President, ILA), ChairmanDr. Patrick J. BrennanDr. Wayne M. MeyersDr. W. Felton Ross (Treasurer, ILA)Dr. R. H. Thangaraj (Secretary, ILA)

CONTRIBUTING EDITORSArgentina: Dr. J. E. Cardama

(Buenos Aires)Australia: Dr. J. C. Hargrave (Darwin)Brazil: Dr. L. M. Bechelli (Sao Paulo)France & Fr. Terr.: Dr. D. Wallach

(Paris)India (North): Dr. L. K. Bhutani

(New Delhi)India (South): Dr. M. Christian

(Karigiri)Israel: Dr. L. Levy (Jerusalem)Japan: Dr. M. Abe (Tokyo)Malaysia & SE Asia: Dr. J. H. S.

Pettit (Kuala Lumpur)

Mexico: Dr. A. Saul (Mexico, D.F.)Netherlands: Dr. D. L. Leiker (Amsterdam)People's Republic of China:

Dr. Ye Gan-yun (Taizhou)Philippines: Dr. R. M. Abalos (Cebu City)Spain: Dr. J. Terencio de las Aguas (Fontilles)United Kingdom: Dr. R. J. W. Rees

(London)U.S.A.: Dr. H. L. Arnold, Jr. (Honolulu)U.S.A.: Dr. P. W. Brand (Carville)U.S.S.R.: Dr. A. A. Juscenko (Astrakhan)Venezuela: Dr. J. Convit (Caracas)West Africa: Dr. M. Nebout (Bamako)

This JOURNAL is indexed in CURRENT CONTENTS/LIFE SCIENCES, BIOLOGICAL ABSTRACTS,and CHEMICAL ABSTRACTS. Microform available from University Microfilms

International, Ann Arbor, Michigan 48106, U.S.A. or London WCIR 4EJ, U.K.

The INTERNATIONAL JOURNAL OF LEPROSY (ISSN 0148-916X) is published quarterly for $60.00 peryear for members and $80.00 per year for subscribers by Allen Press, Inc., 1041 New Hampshire St.,Lawrence, Kansas 66044, U.S.A. Second-class postage paid at Elmwood Park, New Jersey and ad-ditional mailing office. POSTMASTER: Send address changes to INTERNATIONAL JOURNAL OF LEP-ROSY, Business and Circulation Office, One Broadway, Elmwood Park, NJ 07407, U.S.A.

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

CONTENTSVolume 53, Number 1, March 1985

Original Articles^ PageMeyers, Wayne M., Walsh, Gerald P., Brown, Harriet L., Binford, Chapman H., Imes,

George D., Jr., Hadfield, Ted L., Schlegel, Charles J., Fukunishi, Yukiko, Gerone,Peter J., Wolf, Robert H., Gormus, Bobby J., Martin, Louis N., Harboe, Morten, andlmaeda, Tamotsu. Leprosy in a Mangabey Monkey—Naturally Acquired Infection. 1

Cartel, Jean-Louis, Naudillon, Yves, Artus, Jean-Claude, and Grosset, Jacques H ^Hepatotoxicity of the Daily Combination of 5 mg/kg Prothionamide + 10 mg/kg Rifampin.^15

Jesudasan, Kumar and Christian, Melville. Risk of Paucibacillary Leprosy Patients Re-leased from Control Relapsing with Multibacillary Leprosy. ^19

Rook, Graham A. W. Absence from Sera from Normal Individuals or from Rifampin-treatedLeprosy Patients (THELEP Trials) of Antibody to Rifamycin-protein or Rifamycin-membraneConjugates. 22

Chiewsilp, Pimol, Petchclai, Bencha, Chirachariyavej, Thamrong, and Ramasoota, Ter-ra. Immunoglobulins in Leprosy. ^28

Sinha, Sudhir, Sengupta, Utpal, Ramu, Gopal, and lvanyi, Juraj. Serological Survey ofLeprosy and Control Subjects by a Monoclonal Antibody-based Immunoassay. ^33

Narayanan, Rangarajan B., Girdhar, Bhawneshwar K., Sengupta, Utpal, and Desikan,K. V. In vitro Studies on Dermal Granulomas of Human Leprosy—Cellular Characteristics.^39

Datta, Arun K., Katoch, Vishwa Mohan, Sharma, Vishnu Dun, and Katoch, Kiran. Com-parative Study on the Thin-layer Chromatographic Pattern of Methyl Mycolates of M. lepraeand Related Species. 45

Nakamura, Masahiro. Survival of Mycobacterium lepraemurium in vitro for Thirty Years byLyophilization. ^52

Xu, Keyu, de Vries, R. R. P., Fei, Hongming, van Leeuwen, A., Chen, Renbiao, and Ye,Ganyun. HLA-linked Control of Predisposition to Lepromatous Leprosy. ^56

Burchard, Gerd-Dieter and Bierther, Manfred. An Electron Microscopic Study on Macro-phages and Lymphocytes in Lepromatous and Borderline Leprosy. ^64

Burchard, Gerd-Dieter and Bierther, Manfred. An Electron Microscopic Study of the SmallCutaneous Vessels in Lepromatous Leprosy. ^70

Fukunishi, Yukiko. Electron Microscopic Findings of the Peripheral Nerve Lesions of NudeMice Inoculated with M. leprae. ^75

Li, Huan-Ying, Pan, Yu-Lin, and Wang, Yang. Leprosy Control in Shandong Province,China, 1955-1983; Some Epidemiological Features. ^79

EditorialsThe Influence of Immunosuppression and Immunodeficiency on Infections with Lep-

rosy and Tuberculosis. (Nicola Hilary Strickland). ^86The 1984 JOURNAL-a Continuing Perspective. (Robert C. Hastings). ^101

CorrespondenceEffect of Levamisole Therapy on Lepromin Reaction in Lepromatous Leprosy Cases.

(Suresh K. Arora, Gurmohan Singh, and P. C. Sen). ^113Persistence of Langhans' Giant Cells in Rapidly Downgrading Leprosy Lesions. (N. F ^

Lyons, B. P. B. Ellis, and B. Naafs). ^114DDS-resistant Leprosy. (P. N. Neelan and B. N. Reddy). ^115Response to Drs. Neelan and Reddy. (J. G. Almeida and C. J. G. Chacko). ^116Drug Sensitivity Testing of M. leprae. (J. Grosset, L. Levy, R. J. W. Rees, and C. C ^

Shepard). ^118Response to Dr. Grosset, et al. (J. G. Almeida and C. J. G. Chacko). ^120

News and Notes ^122Book Reviews ^129Current Literature ^132First U.S. Hansen's Disease Research Conference, Camille, Louisiana, U.S.A., 1984 ^154Special Grantors and Sustaining Members ^187

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

CONTENTSVolume 53, Number 2, June 1985

Original Articles^ PageWarndorff van Diepen, Titia and Mengistu, Genene. Relapse Rate and Incidence of Dap-

sone Resistance in Lepromatous Leprosy Patients in Addis Ababa: Risk Factors and Effectof Short-term Supplementary Treatment. ^189

Bera, D. K. and Sen, P. C. Effect of Levamisole on Bacterial Index in BL and LL Leprosy. ^198Moncada, B., Baranda, M. L., Gonzàlez -Amaro, R., Urbina, R., and Loredo, C. E ^

Thalidomide—Effect on T Cell Subsets as a Possible Mechanism of Action. ^201Jain, Ganpat Lal, Pasricha, Jagjit Singh, and Guha, Sujoy Kumar. Minimum Temperature

Felt as Hot (MTH)—a New Concept for Grading the Loss of Temperature Sensation inLeprosy Patients. 206

Andreoli, Alberto, Brett, Sara J., Draper, Philip, Payne, Sheila N., and Rook, Graham A W. Changes in Circulating Antibody Levels to the Major Phenolic Glycolipid During Erythe-ma Nodosum Leprosum in Leprosy Patients. 211

de la Barrera, Silvia S., Sasiain, Maria del C., Geffner, J., Isturiz, M. A., Segal -Eiras,Amada, and de Bracco, Maria M. de E. Inhibition of Antibody-dependent, Cell-mediatedCytotoxicity by Serum from Lepromatous Leprosy Patients. ^218

Wright, E. P., Vlug, A., Geertzen, H. G. M., Hoang Thuy Long, and Nguyen Diem Hong ^Serum Immunoglobulins, Including IgG Subclasses, in Vietnamese Leprosy Patients. ^225

Vaishnavi, Chetana, Kumar, Bhushan, Ganguly, Nirmal Kumar, and Kaur, Surrinder Phagocytic and Bactericidal Activities of Macrophages from Mycobacterium /eprae-infectedNormal and Immunosuppressed Mice. 233

Haimanot, Redda Teckle, McDougall, A. Colin, Mshana, Robert N., Andersen, Johs G.,and Belehu, Ayele. Muramidase (Lysozyme) Findings in Sural and Radial Nerve Biopsiesin Leprosy Patients After Varying Periods of Treatment. 238

Fukunishi, Yukiko. Electron Microscopic Findings of Transverse Fission of M. leprae by Freeze-etching Methods. ^247

Bahlinger, Vernon M., Brantley, Phillip J., Madrigal, Daniel R., Heroman, Mary W., andVeitia, Marie C. Psychosocial Stress in Hansen's Disease: A Comparison with Other ChronicIllness Patients. ^251

Coleman, William C., and Madrigal, Daniel R. The Patient with Sensory Loss. ^255Rojas-Espinosa, Oscar, Oltra, Antonina, Arce, Patricia, and Mendez, Ira's. Serum En-

zymatic Changes Following Infection of Mice with Mycobacterium lepraemurium ^258Rojas -Espinosa, Oscar, Quesada -Pascual, F., Oltra, Antonina, Arce, Patricia, Estrada-

Parra, S., and Buchanan, Thomas M. Biochemical Alterations in the Serum of Armadillos(Dasypus novemcinctus) Infected with Mycobacterium leprae. A Preliminary Report. ^262

Baskin, Gary B., Wolf, Robert H., Gormus, Bobby J., Martin, Louis N., Walsh, Gerald P ,Binford, Chapman H., Meyers, Wayne M., and Malaty, Raga. Experimental Leprosyin the Mangabey (Cercocebus atys): Necropsy Findings. 269

EditorialsStrategy for Leprosy Control. (P. H. Lagrange and J. L. Stach) ^278Understanding of Leprosy in Ancient China. (Olaf K. Skinsnes and Peter H. C. Chang) ^289

ObituariesNishiura, Mitsugu ^308Shepard, Charles C. ^309

CorrespondenceAn Unusual Case of Untreated Polar Lepromatous Leprosy Associated with Rare

M. leprae. (Robert H. Gelber) ^311Standardized Schemes for Steroid Treatment in ENL and Reversal Reactions. (F. M ^

J. H. Imkamp) ^313News and Notes ^318Book Reviews ^323Current Literature ^326Special Grantors and Sustaining Members ^364

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

CONTENTSVolume 53, Number 3, September 1985

Original Articles^ PageSeydel, U., Lindner, B., and Dhople, A. M. Results from Cation and Mass Fingerprint Anal-

ysis of Single Cells and from ATP Measurements of M. Leprae for Drug Sensitivity Testing:A Comparison. 365

Lamas -Robles, Carlos, Gonzales -Mendoza, Amado, Pèrez -Suarez, Gloria, and Mejia-Arreguin, Silvia. Use of Human Skin to Demonstrate Antinuclear Antibodies in Lepro-matous Leprosy Patients. ^373

Sathish, M., Rees, R. J. W., Seshadri, P. S., and Nath, Indira. Comparison of RadiometricMacrophage Assay and the Mouse Foot Pad Infection for the Evaluation of Mycobacteriumleprae Sensitivity/Resistance to Dapsone. 378

Longley, Jack, Haregewoin, Abebe, Yemaneberhan, Tabebe, Warndorff van Diepen,Titia, Nsibami, John, Knowles, Daniel, Smith, Kendall A., and Godal, Tore. In VivoResponses to Mycobacterium leprae: Antigen Presentation, Interleukin-2 Production, andImmune Cell Phenotypes in Naturally Occurring Leprosy Lesions. 385

Samuel, N. M., Stanford, J. L., and Desikan, K. V. Microscopic Findings of Delayed Re-actions Elicited by the Skin Test Reagent Leprosin A Derived from M. leprae. ^395

Dugan, Elizabeth, Modlin, Robert L., and Rea, Thomas H. An in Situ lmmunohistologicalStudy of Mitsuda Reactions. ^404

Teruel, J. L., Liano, F., del Hoyo, M., Rocamora, A., GOmez Mampaso, E., Quereda, C.,and Ortuno, J. Successful Kidney Transplantation in Leprosy and Transitory Recurrenceof the Disease. 410

Douglas -Jones, A. G. and Watson, J. D. Immunity to Leprosy. 1. The Proliferative Responseof Murine T Lymphocytes to Mycobacterium leprae. ^412

Negate, Yoko, Kohsaka, Kenji, and Ito, Tonetaro. Negative Observations on Immunolog-ical Side Effects of Rifampin and Dapsone in Mice. ^421

Datta, Geeta, Naik, Sharad S., and Gurnani, Shantoo. Chemical Characterization of theDapsone Binding Site of Lysozyme. ^428

Hirata, Tsunehiko. Electron Microscopic Observations of Cell Wall and Cytoplasmic Mem-brane in Murine and Human Leprosy Bacilli. ^433

Larsson, Lennart, Draper, Philip, and Portaels, Francoise. Use of Gas Chromatographyto Differentiate Mycobacterium leprae from Cultivable Armadillo-derived Mycobacteria, M.avium/intracellulare, and M. lepraemurium by Analysis of Secondary Alcohols. 441

Fukunishi, Yukiko, Kearney, George P., Whiting, John, Jr., Walsh, Gerald P., Meyers,Wayne M., and Johnson, Frank B. Isolation of Characteristic Glycolipids Possibly In-cluded in Spherical Droplets Around M. leprae. 447

Date, Anand, Harihar, Seshadri, and Jeyavarthini, S. Elizabeth. Renal Lesions and OtherMajor Findings in Necropsies of 133 Patients with Leprosy. ^455

Lechat, Michel F., Misson, Claudine B., Lambert, Andre, Bouckaert, Andre, Vanderve-ken, Mark, and Vellut, Claire. Simulation of Vaccination and Resistance in Leprosy Usingan Epidemiometric Model. 461

EditorialsLeprosy in Young Children—Past, Present and Future. (M. E. Duncan)

^468

Patient Compliance in Leprosy Control: A Necessity in Old and New Regimens. (HanHuikeshoven)

^474

CorrespondenceEvidence for the Activity of Rifampin on the Neuropathy of Foot Pad-inoculated Mice

with Mycobacterium leprae. (Alain Sebille, Marie-Odile Crepon, Claire-Cecile Guelpa,and Jacques Grosset) 481

NADH-Methemoglobin Reductase and Reticulocytosis. (Omar Sergio Caticha -Alfonso)^483Purification of Phenolic Glycolipid I from Armadillo and Human Sources. (Shirley Wu

Hunter, Carol Stewart, Patrick J. Brennan) ^484Isolation of Phenolic Glycolipid I from Human Lepromatous Nodules. (Nalini Vemuri,

Lakshmi Khandke, P. R. Mahadevan, Shirley W. Hunter, and Patrick J. Brennan) ^487News and Notes ^490Book Reviews ^491Current Literature ^493Special Grantors and Sustaining Members ^516

INTERNATIONAL JOURNAL OF LEPROSYand Other Mycobacterial Diseases

CONTENTSVolume 53, Number 4, December 1985

Original Articles^ PageBrubaker, Merlin L., Meyers, Wayne M. and Bourland, Jacques. Leprosy in Children One Year of Age

and Under. ^517Sommerfelt, Halvor, Irgens, Lorentz M. and Christian, Melville. Geographical Variations in the Occur-

rence of Leprosy: Possible Roles Played by Nutrition and Some Other Environmental Factcis ^524Heckel, Christine and Beiguelman, Bernardo. Chromosomal Aberrations in Cultures of Skin Fibroblasts

of Leprosy Patients. ^533Petri, Valeria, Mendes, Ernesto, V. and Beiguelman, Bernardo. Histology of the Mitsuda Reaction of

Healthy Adults with No Known Contacts with Leprosy Patients. ^540Waters, Michael F. R. and Stanford, John L. Giant Reactions to Tuberculin in Lepromatous Leprosy

Patients. ^546Yoder, Leo J., Jacobson, Robert R. and Job, Charles K. A Single Skin Lesion—An Unusual Presentation

of Lepromatous Leprosy. ^554Brown, Arthur E., Nelson, Kenrad E., Makonkawkeyoon, Sanit, Vithayasai, Vicharn, Scollard, David

M. and Bullock, Ward E. A Study of the Immunological Effects of Cimetidine in Patients with Lepro-matous Leprosy. ^559

Wu, Oin -xue, Ye, Gan -yun, Zhou, Li - lin, Shu, Hui -wen, Liu, 0i, Li, Xin -yu, Ma, Zhou -xiang and Li,Zhi -wen. Determination of Antibodies in Dried Blood from Earlobes of Leprosy Patients by Enzyme-linked Immunosorbent Assay—A Preliminary Report. ^565

Mukherjee, Ashok, Misra, Radhey S. and Sharma, Amar K. Ultrastructure of Leprous Phlebitis. ^571Hirata, Tsunehiko. Electron Microscopic Observations of Acid-fast Bacilli in Nasal Mucosal Biopsies of

Lepromatous Leprosy Patients. ^577Cree, Ian A., McDougall, A. Colin, Coghill, George and Beck, J. Swanson. Quantitation of the Granu-

loma Fraction in Leprosy Skin Biopsies by Planimetry. ^582Shepard, Charles C., Jenner, Peter J., Ellard, Gordon A. and Lancaster, R. Denise. An Experimental

Study of the Antileprosy Activity of a Series of Thioamides in the Mouse. ^587Baline, Luis M., Valdez, Rani P., de Herrera, Miguel, Costa Cordova, Horacio, Bellocq, Juan and

Garcia, Norberto. Experimental Reproduction of Leprosy in Seven-banded Armadillos (Dasypushybridus). 595

Mori, Tetsuo, Miyata, Yasuyo, Kohsaka, Kenji and Makino, Masanao. Respiration in Mycobacteriumleprae. ^600

EditorialsSecular Trends in Leprosy: Increase in Age at Onset Associated with Declining Rates and Long

Incubation Periods. (Lorentz M. Irgens) ^610Monoclonal Antibodies and Recombinant DNA Technology: Present and Future Uses in Leprosy

and Tuberculosis. (Michael J. Seckl) ^618

ObituaryPedley, James Cecil ^641

CorrespondenceSilastic Implant of the First Dorsal Interosseous Space. (Alan G. Zacharia and Robert H. Gelber) ^643Exfoliative Dermatitis After Clofazimine. (K. Pavithran) ^645Double-stranded DNA Inhibits Cardiolipin-binding Activity in Lepromatous Leprosy Patients' Sera ^

(Fukumi Furukawa, Gakuji Ohshio and Yoshihiro Hamashima) ^647Interleukin-2-induced T-cell Response to M. leprae in Lepromatous Leprosy: Reversion of a Sup-

pressor Mechanism or Expansion of a Small M. leprae-reactive T-cell Pool? (Premlata Shankar,Daniel Wallach and Marie -Anne Bach) ^649

Distribution of Mycobacterium leprae in the Circles of Counting Slides. (Charles C. Shepard andLouis Levy) ^653

Distribution of Mycobacterium leprae in Circular Counting Films. (David P. Humber) ^656Nilayam Report. (T. N. Jagadisan) ^658

News and Notes ^659Book Reviews ^663

Current Literature ^666

U.S.—Japan Cooperative Medical Science Program, Twentieth Joint Leprosy Re-search Conference, Bethesda, Maryland, U.S.A., 1985 ^700

Reviewers ^729

International Leprosy Association Officers and Councillors ^730

Statement of Financial Condition ^731

Statement of Ownership, Management, and Circulation ^735

Special Grantors and Sustaining Members ^737