histochemical analysis

8/13/2019 Histochemical Analysis

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Histochemical analysis

Histochemistry is the study of the identification and distribution of chemical compounds within and between biological cells using histological techniques such as histology stains , indicators and light (optical) and electron microscopy.

Histology stains are usually selected according to the type of biological tissue to beobserved. There are many different histology stains in use.Some histology stains are more widely used than others. Some histology stains are onlyused to study very specific types of biological tissue.

E amples of some histology stains and some categories of histology stains are listed inalphabetical order below, with brief notes about each. This list is intended to convey anindication of the wide range of histology stains in use in histology laboratories.

!ldehyde "uchsin

# can be used to stain pancreatic islet beta cell granules

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!lician %lue

# a &ucin stain (a category of histology stains, listed below)# can stain mucins and mucosubstances blue (due to the copper in the stain)

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!li arin ed S

# can be used to identify calcium in tissue sections# used on the *upont !+! analy er to measure serum calcium photometrically

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!l-aline hosphatase

# can be used to stain endothelial cells

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! an Stain

# can be used to differentiate osteoid from mineralised bone
http://www.ivy-rose.co.uk/Chemistry/GCSE/What-is-a-compound.phphttp://www.ivy-rose.co.uk/HumanBody/Histology/Histology-Stains.phphttp://www.ivy-rose.co.uk/HumanBody/Histology/Histology-Stains.phphttp://www.ivy-rose.co.uk/HumanBody/Eye/What-is-Light.phphttp://www.ivy-rose.co.uk/Chemistry/GCSE/What-is-a-compound.phphttp://www.ivy-rose.co.uk/HumanBody/Histology/Histology-Stains.phphttp://www.ivy-rose.co.uk/HumanBody/Eye/What-is-Light.php


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%ielschows-y Stain

# can be used to show reticular fibres

# used for showing neurofibrillary tangles and senile plaques# uses the chemical element silver (!g)

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+a2al Stain

# can be used on nervous tissue .

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+ongo ed

# used to stain amyloid fibres (to appear orange4red).

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+resyl 6iolet

# will stain both neurons and glia # bonds with acidic parts of cells such as ribosomes , nuclei and nucleoli

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Eosin

# commonly used for general histology staining when paired with haemato ylin # seeHemato ylin and Eosin (H9E)

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"ontana#&asson

# uses the chemical element silver (!g)# stains argentaffin granules and melanin blac- # while also staining nuclei pin-4red andcytoplasm light pin- # a specific e ample of a &elanin Stain (general category of histology stains)

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:iemsa Stain
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# a omanows-i (also written ; omanows-y;) type stain# used for peripheral blood smears, i.e. a thin layer of blood smeared on a microscopeslide and used for bone marrow.# used to study parasites and malaria

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:olgi Stain

# can be used to stain neurons

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:omori Trichrome

# trichrome histology stains are formed from a mi ture of three dyes

# :omori


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histochemical analysis

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