high chlorophyll concentraons & phytoplankton composi

Sparkling Lake, Vilas County, WI Background > Sparkling Lake has high chlorophyll concentra<ons under-ice compared to other lakes in the region. > Chlorophyll can be an indicator of produc<vity and lake health. In this study, we inves<gate how chlorophyll concentra<ons correlate with phytoplankton composi<on. Methods February (9) March (1) April (8) May (5) June (9) July (11) August (11) November (11) Phytoplankton samples are hypsometrically pooled and preserved in Lugol’s solu<on. Water samples are then sent to PhycoTech Labs to be placed on slides. 1 Emily C. Whitaker 1 , Thomas Shannon 1 , and Hilary A. Dugan 1 Center for Limnology, University of Wisconsin-Madison 1 Figure 1: Light ex<nc<on rela<onship with snow depth for North-Temperate Lakes. DoXed line at 5cm of snow where light drama<cally starts to decrease. % Epilimnion % Hypolimnion > Star<ng in 1983, phytoplankton samples have been collected four <mes a year. Chlorophyll samples are collected biweekly to monthly by the NTL-LTER. > Previously unexamined slides were analyzed for community composi<on using microscopy. Indicator phytoplankton genus were iden<fied. > Cryptomonad: common indicator species > Cyclotella: grow in sustained periods of darkness > Fragilaria: indicators of early spring bloom > Planktothrix: free floa<ng species > Naked /Armored Dinoflagellates: frequent across <me series, looking to parse differences > Limnothrix: contain enzymes in the cyanobacterial carbon metabolism > Cyanobacteria: Watching for blooms & busts @EmilyCWhitaker Results hXps://emilywhitaker.github.io High Chlorophyll Concentra<ons & Phytoplankton Composi<on Under Lake Ice > Sparkling Lake is a 63 ha, oligotrophic seepage lake in north-temperate Wisconsin. Acknowledgments: National Science Foundation, NTL-LTER#DEB-1440297. Department of the Interior Northeast Climate Adaptation Science Center Figure 3: Cell counts of phytoplankton in our <me series (A) in comparison to biovolume calcula<ons (B). Cell counts gave rough es<mates of what was occurring in both seasons and biovolume keyed into finite changes and showed “missing” genera. Figure 2: Surface chlorophyll during ice on compared to ice off. Note more data has been collected during ice off than ice on (576 points vs. 121). Ice Ice off Ice on (n=121) vs. Ice off (n=576) Chlorophyll (ug/L) A B

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Page 1: High Chlorophyll Concentraons & Phytoplankton Composi

SparklingLake,VilasCounty,WI

Background

>SparklingLakehashighchlorophyllconcentra<onsunder-icecomparedtootherlakesintheregion.>Chlorophyllcanbeanindicatorofproduc<vityandlakehealth.Inthisstudy,weinves<gatehowchlorophyllconcentra<onscorrelatewithphytoplanktoncomposi<on.

Methods

February(9)

March(1)

April(8)

May(5)

June(9)

July(11)

August(11)

November(11)

PhytoplanktonsamplesarehypsometricallypooledandpreservedinLugol’ssolu<on.WatersamplesarethensenttoPhycoTechLabstobeplacedonslides.

EmilyC.Whitaker1,ThomasShannon1,andHilaryA.Dugan1CenterforLimnology,UniversityofWisconsin-Madison1

Figure1:Lightex<nc<onrela<onshipwithsnowdepthforNorth-TemperateLakes.DoXedlineat5cmofsnowwherelightdrama<callystartstodecrease.

%Epilimnion

%Hypolimnion

>Star<ngin1983,phytoplanktonsampleshavebeencollectedfour<mesayear.ChlorophyllsamplesarecollectedbiweeklytomonthlybytheNTL-LTER.>Previouslyunexaminedslideswereanalyzedforcommunitycomposi<onusingmicroscopy.Indicatorphytoplanktongenuswereiden<fied.

>Cryptomonad:commonindicatorspecies>Cyclotella:growinsustainedperiodsofdarkness>Fragilaria:indicatorsofearlyspringbloom>Planktothrix:freefloa<ngspecies>Naked/ArmoredDinoflagellates:frequentacross<meseries,lookingtoparsedifferences>Limnothrix:containenzymesinthecyanobacterialcarbonmetabolism>Cyanobacteria:Watchingforblooms&busts

@EmilyCWhitaker

Results

hXps://emilywhitaker.github.io

HighChlorophyllConcentra<ons&PhytoplanktonComposi<onUnderLakeIce

>SparklingLakeisa63ha,oligotrophicseepagelakeinnorth-temperateWisconsin.

Acknowledgments: National Science Foundation, NTL-LTER#DEB-1440297. Department of the Interior Northeast Climate Adaptation Science Center

Figure3:Cellcountsofphytoplanktoninour<meseries(A)incomparisontobiovolumecalcula<ons(B).Cellcountsgaveroughes<matesofwhatwasoccurringinbothseasonsandbiovolumekeyedintofinitechangesandshowed“missing”genera.

Figure2:Surfacechlorophyllduringiceoncomparedtoiceoff.Notemoredatahasbeencollectedduringiceoffthaniceon(576pointsvs.121).

Ice IceoffIceon(n=121)vs.Iceoff(n=576)

Chloroph

yll(ug/L)

A B

MODIS Chlorophyll Fluorescence

Chlorophyll Fluorescence and Photosynthesis

CHLOROPLAST AND CHLOROPHYLL

Chlorophyll Extraction Poster

Chlorophyll Biosynthesis

Chlorophyll triplets and radical pairs - uni-muenchen.de · 2012-05-22 · 4. Chlorophyll in Liquid Crystals 956 B. Aggregates 956 1. Chlorophyll Dimers 956 2. Chlorophyll in Micelles

Liquid chlorophyll leaflet_uk