SparklingLake,VilasCounty,WI
Background
>SparklingLakehashighchlorophyllconcentra<onsunder-icecomparedtootherlakesintheregion.>Chlorophyllcanbeanindicatorofproduc<vityandlakehealth.Inthisstudy,weinves<gatehowchlorophyllconcentra<onscorrelatewithphytoplanktoncomposi<on.
Methods
February(9)
March(1)
April(8)
May(5)
June(9)
July(11)
August(11)
November(11)
PhytoplanktonsamplesarehypsometricallypooledandpreservedinLugol’ssolu<on.WatersamplesarethensenttoPhycoTechLabstobeplacedonslides.
1
EmilyC.Whitaker1,ThomasShannon1,andHilaryA.Dugan1CenterforLimnology,UniversityofWisconsin-Madison1
Figure1:Lightex<nc<onrela<onshipwithsnowdepthforNorth-TemperateLakes.DoXedlineat5cmofsnowwherelightdrama<callystartstodecrease.
%Epilimnion
%Hypolimnion
>Star<ngin1983,phytoplanktonsampleshavebeencollectedfour<mesayear.ChlorophyllsamplesarecollectedbiweeklytomonthlybytheNTL-LTER.>Previouslyunexaminedslideswereanalyzedforcommunitycomposi<onusingmicroscopy.Indicatorphytoplanktongenuswereiden<fied.
>Cryptomonad:commonindicatorspecies>Cyclotella:growinsustainedperiodsofdarkness>Fragilaria:indicatorsofearlyspringbloom>Planktothrix:freefloa<ngspecies>Naked/ArmoredDinoflagellates:frequentacross<meseries,lookingtoparsedifferences>Limnothrix:containenzymesinthecyanobacterialcarbonmetabolism>Cyanobacteria:Watchingforblooms&busts
@EmilyCWhitaker
Results
hXps://emilywhitaker.github.io
HighChlorophyllConcentra<ons&PhytoplanktonComposi<onUnderLakeIce
>SparklingLakeisa63ha,oligotrophicseepagelakeinnorth-temperateWisconsin.
Acknowledgments: National Science Foundation, NTL-LTER#DEB-1440297. Department of the Interior Northeast Climate Adaptation Science Center
Figure3:Cellcountsofphytoplanktoninour<meseries(A)incomparisontobiovolumecalcula<ons(B).Cellcountsgaveroughes<matesofwhatwasoccurringinbothseasonsandbiovolumekeyedintofinitechangesandshowed“missing”genera.
Figure2:Surfacechlorophyllduringiceoncomparedtoiceoff.Notemoredatahasbeencollectedduringiceoffthaniceon(576pointsvs.121).
Ice IceoffIceon(n=121)vs.Iceoff(n=576)
Chloroph
yll(ug/L)
A B
