h istology 4 types: epithelial connective muscle nervous 1 study of tissues -cells similar in...
TRANSCRIPT
HISTOLOGY
4 types:
• Epithelial
• Connective
• Muscle
• Nervous
1
Study of tissues -cells similar in structure and perform a common function
WHAT IS NECROSIS?
Necrosis is tissue death. It occurs if the blood supply to an area is stopped for too long. When this happens the tissues die due to a lack of oxygen to the cells.
2
PREPARING TISSUES FOR MICROSCOPY
Fixed – preserved Artifacts – distortions not seen in living
tissue Sectioned – cut into slices thin enough
light can pass through Stained – organic dyes used to
enhance contrast
Transmission electron microscopy (TEM) – uses electrons for detailed contrast
Scanning electron microscopy (SEM) – 3D pictures of tissue surface
3
EPITHELIAL TISSUE Covers a surface or lines a cavity Glandular epithelium – exocrine glands Forms boundaries
Basement Membrane: Network of protein fibers that forms barrier between epithelium and connective tissue
Protection Absorption – molecules pass into blood or
lymph Filtration – passage of solvent thru a
membrane Excretion – eliminate waste Secretion – release aqueous solution Sensory reception
4
CHARACTERISTICS OF EPITHELIUM Polarity
Apical surface – exposed Microvilli
Increase surface area Allow absorption and secretion
Cilia Beat to move materials across surface
Basal Surface – attached Basal lamina – adhesive glycoproteins
Specialized Contacts Tight Junctions
Prevents passage of water and solutes Interlocking membrane proteins Basal adhesion belt binds cells together
Desmosomes Durable interconnections 2 types:
Button desmosomes- small disk Hemidesmosomes – half button shape
5
MORE CHARACTERISTICS OF EPITHELIUM
Supported by connective tissue Reticular lamina – Extracellular collagen network
Avascular but innervated No blood vessels, nutrients obtained from diffusion Nerve supply
Regeneration Cells are continually replaced Epithelial cells only survive 1-2 days
7
SQUAMOUS EPITHELIUM
Simple SquamousThin and flat cells, 1 LayerFunction: diffusion & filtrationFound: forms capillary walls
(endothelium), lining air sacs of lungs, kidneys, lining body cavities (mesothelium)
Stratified SquamousThin and flat cells, More than 1
layer, most common epitheliaFunction: protectionFound: in places of mechanical
stress, skin surface, lining tongue, mouth, esophagus, and anus 8
CUBOIDAL EPITHELIUM
Simple Cuboidal Box shaped cells, 1 layer Function: Secretion or
absorption Found: covering surface of
ovaries, lining kidney tubules, salivary ducts, and pancreatic ducts
Stratified Cuboidal Box shaped cells, 2 layers, rare Function: strengthen lumen
walls Found: ducts of large sweat
glands, salivary glands, mammary glands, and pancreas 9
COLUMNAR EPITHELIAL
Simple Columnar Tall, rectangular cells, 1 layer Function: absorption
(microvilli) & secretion Found: Lines stomach,
intestinal tract, excretion ducts, gall bladder
Pseudostratified Columnar Single layer irregularly
shaped cells (looks like multiple layers)
Function: protection, secretion
Found: Lining respiratory passageways (ciliated), and auditory tubes 10
TRANSITIONAL EPITHELIAL TISSUE
Transitional3-6 layers of rounded
cellsFunction: Withstands
stretchingFound: Lines urinary
bladder and portions of ureters and urethra
11
EXOCRINE GLANDULAR EPITHELIA
Exocrine glands: excrete secretions usually thru ducts onto surface Unicellular
Goblet & mucous cells scattered among epithelial cells
Secrete mucin (glycoproteins & water) via exocytosis
Multicellular – Secretory sheet, classified by pattern of ducts
ex – mucin lining stomach
Mode of Secretion: Merocrine: via exocytosis
Ex. Skin Perspiration, Mucus Glands, Saliva, mammary glands (milk)
Apocrine: lose cytoplasm and secretory product Ex. Mammary glands (lipids)
Holocrine – cell fills with secretion then bursts and dies Ex. Sebaceous glands – oils from base of hair
12
13
CLASSIFICATION OF CONNECTIVE TISSUES: 3 TYPESConnective Tissue Proper
Syrupy ground matrixEx. Loose (areolar, adipose) & Dense
(tendons & ligaments)Blood - Fluid Connective tissue
Cells suspended in watery ground substance w/dissolved proteins
Cartilage Supporting Connective TissueDense ground substance(Hyaline, Elastic, Fibrocartilage)
Bone Supporting Connective Tissue14
CONNECTIVE TISSUES
3 Main Components Specialized cells Mostly nonliving extracellular matrix that surrounds
cells Protein Fibers – collagen, elastic, reticular Ground Substance (fluid) – fills space between cells
Interstitial fluid, Cell Adhesion Proteins, Proteoglycans
Functions (vary widely) Binding & Supporting - framework Transport materials – fluid with dissolved material Storing Energy– fat in adipose tissue Insulating Protect Organs
15
CONNECTIVE TISSUE PROPER: CELL TYPES
Fibroblasts Produce and maintain connective tissue fibers
and ground substance Local maintenance & repair permanent
Macrophages Defense: Engulf damaged cells and pathogens Release chemicals to stimulate immune
response Fixed or migrating (reinforcement)
Adipocytes Permanent fat cells store nutrients Droplet of lipid pushes nucleus to side
Mast Cells Mobile, found near blood vessels Have vesicles filled with chemicals to be
released after an injury or infection Heparin -anticoagulant Histamine – leaky capillaries Proteases & other enzymes 16
CONNECTIVE TISSUE FIBERS
CollagenLong, straight,
unbranched, flexible Elastic
Protein – elastinWavy, branched,
stretchy Reticular Fibers
Thin, branching interwoven network
17
18
GROUND SUBSTANCE
Fills spaces between cells, surrounds fibers Connective tissue proper – clear, colorless,
syrupy to slow movement of pathogens
19
LOOSE CONNECTIVE TISSUE Areolar Tissue
Contains all cells and fibers of connective tissue proper
Separates skin from muscles Provides padding Allows movement Extensive blood supply
Adipose tissue- 90% adipose cells Behind eyes, kidneys, heart,
abdomen, buttocks, and breasts
Reticular Tissue- dominated by reticular fibers Lymph nodes, bone marrow, and
spleen
20
WHAT IS THE DIFFERENCE BETWEEN WHITE FAT AND BROWN FAT?
White fat Pale yellow in color Found in adults Used for insulation, long term energy storage, &
cushion Brown fat
Highly vascular, contains lots of mitochondria Found in infants and young kids Metabolically very active, breaks down lipids fast Instead of absorbing energy, it releases heat to
warm circulating blood to increase body temperature 21
DENSE (FIBROUS) CONNECTIVE TISSUEConsists of mostly collagen fibers Dense Regular – collagen
fibers are parallel to resist tension
Poorly vascularized Tendons – connect skeletal
muscle to bone Aponeuroses – flat
tendons (muscle to muscle or bone)
Ligaments – contain elastic fibers, connect bone to bone
Fascia – binds together muscles, bv, and nerves
Dense Irregular – meshwork of thick collagen fibers Provides support in many
directions (i.e. skin dermis) Joint and organ capsules
22
FLUID CONNECTIVE TISSUES
BloodPlasma- watery matrix
w/dissolved proteinsRed blood cell (rbc)White blood cells (wbc)Platelets
23
CARTILAGE SUPPORTING CONNECTIVE TISSUE:
Cartilage – gel w/embedded fibersChondrocytes – cells found in lacunae derived
from chondroblastsAvascular, therefore difficult to repairLacks nervesTypes:
Hyaline – tightly packed collagen fibers, tough but flexible Connects ribs to sternum, supports passageways of respiratory
tract, covers bone surfaces in joints, tip nose, embryonic skeleton Elastic – elastic fibers, resilient and flexible
Flap of outer ear (pinna), epiglottis, auditory tube Fibrocartilage – mostly collagen fibers, durable and tough
Between vertebrae, between pubic bones, around or within joints24
Hyaline Cartilage
Elastic Cartilage
Fibrocartilage
25
BONE SUPPORTING CONNECTIVE TISSUE:
Osseous tissue: Bone
Matrix – hard calcium and flexible collagen, very little ground substance
Osteocytes found w/in lacunae
Lacunae surround blood vessels
Canaliculi extend from central (Haversian) canal 26
Osseous Tissue
27
MUSCLE TISSUE Muscle tissue – interaction between the
myofilaments myosin and actin create a contraction, highly vascular
Skeletal – striated voluntary muscle Large, multinucleated cells (long and slender) Incapable of dividing, but produced through stem cells Striations (series of bands) Only contract when stimulated by nerves
Cardiac – striated involuntary muscle Smaller striated cells, single nucleus Interconnected by intercalated discs Limited ability to repair Pacemaker cells establish a regular rate of contraction
Smooth – nonstriated involuntary muscle Walls of blood vessels, hollow tube=like organs Small, slender cells w/ one nucleus Actin and myosin are scattered so no striations Can be repaired Can contract on own or by nervous system 28
NERVOUS TISSUE Specialized for conducting electrical impulses
and responding to stimuli 2 Types Cells:
Neurons – communicate thru electrical events Neuralgia – physical support for neural tissue,
supply nutrients to neurons
Long cells w/ 3main parts: Cell body w/ nucleus Dendrites – branching projections that receive info Axon – long projection (w/synaptic terminals)
relays info to other cells Limited ability to repair
29
30
MEMBRANES Membranes - epithelia and connective
tissues combine to form 4 types of membranes: Mucous Membranes Serous membranes Cutaneous membranes Synovial membranes
31
CUTANEOUS MEMBRANE
Aka Skin – covers surface of body, thick, dry
Stratified squamous epithelium and underlying dense connective tissue
32
MUCOUS MEMBRANES
Mucosae – line cavities with exterior contactDigestive, respiratory, reproductive, and
urogentital tracts Epithelial surfaces kept moist Ex.
Simple columnar epithelia of dig. tractStratified squamous epithelia of mouthTransitional epithelia of urinary tract
33
SEROUS MEMBRANES
Minimizes friction when organ moves Line internal subdivisions of ventral body
cavityParietal – lines inner surface of cavityVisceral – lines outer surface or organs
Simple epithelia supported by loose connective tissuePleura – covers pleural cavity and lungsPeritoneum – lined abdominal cavity and
associated organsPericardium – lines pericardial cavity and heart
34
SYNOVIAL MEMBRANES
Lines articulation (joint) capsule Loose connective tissue and incomplete layer
of epithelial tissue Produce synovial fluid for smooth movements
35
Synovial Membrane
Cutaneous Membrane
Mucous Membrane
Serous Membrane
36
TISSUE INJURY AND REPAIR Inflammation
In response to pathogens, impact, abrasion, extreme temperatures, or chemical irritation
Mast cells release histamine and heparin that dilate blood vessels to induce swelling, warmth, redness and pain
RegenerationFibroblasts produce dense collagen fibers
called fibrous tissue (scar tissue) Fibrosis is the permanent replacement of
normal tissues with fibrous tissue (i.e. heart and muscle tissues) 37
TISSUES AND AGING
Speed and effectiveness of tissue repair decreases with age (change of hormones and lifestyle)
Epithelia gets thinner, bones become brittle, cardiac muscle fibers and neurons cannot be replaced
Osteoporosis – inactivity, low calcium, and decrease in estrogen result in poor bone strength
38