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FIRST RESPONDERSFIELD GUIDE

ALIRT:NOT IF

BUT WHEN

ALIRTFIRST RESPONDERS

FIELD MANUAL AND SUPPLEMENTAL INFORMATION

TABLE OF CONTENTS

Agenda Training Programs April 20 and May 14, 2005Response Kit ContentsEducational Supplements

ALIRT FIRST RESPONDERS FIELD MANUAL

INTRODUCTION TO INCIDENT COMMAND SYSTEM

EPIDEMIOLOGICAL INVESTIGATION

SAMPLING PROCESS AND NECROPSY

CRIME SCENE GUIDELINES

TREATMENT, EUTHANASIA AND DISPOSAL

CLEANING AND DISINFECTING

MISCELLANEOUS SUPPLEMENTAL INFORMATIONISSUES IN AGRICULTURAL BIOTERRORISM

USEFUL WEB PAGES

SUBMISSION FORMS Az VDL

ALIRT FIELD MANUAL AND SUPPLEMENTAL GUIDE 2ND EDITIONALIRT COMMITTEE

ALIRT CommitteeDr. Rick Willer, Dr. Bob Kattnig, C. B. “Doc” Lane, Basillo “Bas” Aja Jr., Tom Chilton, DaveHartman, Mark Lutman, Dr. S. Peder CuneoArizona Veterinary Diagnostic Laboratory: Dr. Greg Bradley, Director, Dr. Sharon Dial, Dr.Jennifer Johnson, Darcy Kennedy

Training Program ALIRT FIRST RESPONDERS

University of Arizona Veterinary Diagnostic LaboratoryWednesday April 20 and Tuesday May 14, 2005

9:00-9:15 Arrival AzVDL Registration, Introduction Az DOA and AzVDL Faculty

9:15-10:15 Orientation to ALIRT team processPayment for services

Dr. Willer Incident response (introduction to FEMA ICS system)Communication systems (use of extension data ports, communication withALIRT Committee)Review of ALIRT kits and Educational Materials

Dr. Cuneo

10:15-11:00 Review of common causes of extensive livestock deathDr. Bradley

11:00 11:15 Break

11:15 -12:00 Handling Possible FAD’s Az DOA and USDA APHISWhat State and Federal agencies will do if incident is FADDr. Willer and APHIS VS

High Risk FAD’s and Possible WMD’s, Educational PackagesDr. Cuneo

12:00-1:00 Lunch (on your own) (1:00-1:15) Tour Az VDL

1:15-2:15 Sample handling as evidence University of Az Police

2:15-2:45 Sample collection/ Processing/ ShipmentHow to handle samples collected in the field, further processing and shipment toAzVLD

Dr Dial

3:00-4:00 ALIRT Field Investigation Sample CollectionDr Johnson

4:00-4:15 Handout ALIRT Kits/Educational KitsContinuing Education Certificates

Arizona ALIRTVeterinary First Responders

Field Investigation Kit

1 ALIRT kit travel bag1 box disposable exam gloves1 bag disposable shoulder exam gloves5 pair disposable boot covers2 pair Teyvek coveralls1 pair safety glasses4 surgical face maskssmall plastic bottle Novasan, personal C and D1.3 oz packets Virkon (5)1 boot brushhead lampAA Batteries (box 24)

2 necropsy knives1 E-Z Lappruning lopperslarge ax2 #3 scalpel handles with disposable bladesScissors

1 pair sharp/sharp1 pair curved blunt/blunt

2 pair large thumb forceps, rat tooth2 marking penspH test strips (1 tube 200)nitrate test strips (1 tube 50)blood collection Monovette

25 9ml serum20 9ml heparin

4 large Nalgene (water collection)4 large paper bags (plant collection)10 Pur-Wraps cotton applicators (viral isolation)20 Sterile transport swabs (Amies w/o charcoal)Whirlpak

20 55oz (brain samples)20 4oz (other tissue samples)

Zip Lock20 1gallon ( use with 55 oz Whirlpak)20 8x10 (use with 4 oz Whirlpak)

1 box evidence tapesmall zip lock with cable ties2 38x48 4mil red plastic biohazard bags with ties

1digital cameraCannon Power Shot A85Flash Memory Card 512 MB3 Piece Lens Cleaning KitCamera Bag

1 GPSGarmin Rino 120 2 way radio and GPS

ALIRTVeterinary First Responders

Educational Materials

ALIRT First Responders Procedures Manual and Supplemental InformationAz VDL Faculity

Necropsy ExaminationCattle Disease Guide

Palo Duro Consultation, David Bechtol DVM

Foreign Animal Diseases (the Grey Book) United States Animal Health Association

A Guide to Plant Poisoning of Animals In North America with CD Anthony P. Knight

Large Animal User’s Guide California Health and Food Safety Laboratory System

Emerging and Exotic Diseases of Animals Iowa State University

Practical Necropsy Guide Elanco Animal Health

Arizona Atlas & Gazetteer DeLorme

Food Security The Threat to American Livestock USDA/APHIS CD

Disaster Resources Handbook University of Missouri Extension CD

ALIRT FIELD MANUAL

TABLE OF CONTENTS

Introduction to ALIRT pg 3-5ALIRT Response pg 6-8ALIRT Conference Call & Contact Numbers pg 9-10

ALIRT Field Response, Introduction pg 11Response Flow Chart pg 12Sample Report Form pg 13-15

Foreign Animal Disease Information pg 16-20Mandatory Reporting Form pg 21

Crime Scene Information pg 22

Sampling ProceduresLive Animal pg 23Necropsy pg 24-28 Water, Forage & Plants pg 29-30

Sample Shipping pg 31-37

Introduction and Set Up GPS pg 38-41

ALIRT Field Investigation Report Form (2 copies) pg 42-44 & 45-47

ALIRT FIELD MANUAL:ALIRT CommitteeDr. Rick Willer, Dr. Bob Kattnig, C. B. “Doc” Lane, Basillo “Bas” Aja Jr., Tom Chilton, DaveHartman, Mark Lutman, Dr. S. Peder CuneoArizona Veterinary Diagnostic Laboratory: Dr. Greg Bradley, Director, Dr. Sharon Dial, Dr.Jennifer Johnson, Darcy Kennedy

November 30, 2005

ALIRTFIELD MANUAL

INTRODUCTION

What is ALIRT?

A ArizonaL LivestockI IncidentR ResponseT Team

What Is the Mission of ALIRT?

Respond to Unexpected Livestock Losses in Arizona

Provide Trained First Responders to Start Gathering Data and Information to Assist in TimelyDiagnosis

To Insure Rapid and Effective Communication With ALIRT Members and Producers

To Assist in Reducing Further Livestock Losses and help Mitigate Effects on Surviving AnimalsMaintain database of causes of livestock losses

What causes the development of ALIRT?

Agriculture (livestock) has been identified as a critical part of the United States infrastructure byPresidential directive

Extensive management of range livestock in Arizona

Problems of time, distance and expense in obtaining veterinary assistance

Diagnosis in many cases was difficult due to delay in responseDelay in producer identificationDelay in response

Poor communication and interaction between producers, responders and diagnostic servicesResponders not equipped and/or trainedSamples incorrectly obtained and/or processedSamples not sent to correct diagnostic services, samples lostResults not returned in a timely manner or not sent to correct responders

What are the Goals for ALIRT?

Establish the ALIRT Committee

To develop a core of trained and equipped first responders

To correctly:COLLECTTRANSPORTEVALUATEDiagnostic samples collect in field investigations by first responders

What are the responsibilities of the ALIRT Committee?

To develop and implement the ALIRT team

To evaluate livestock incidents and determine the type of ALIRT response

To over watch ALIRT responses and insure all necessary diagnostic and support efforts areavailable to producers and first responders

Provide communication between producers, ALIRT team responders and diagnostic services

Update training and response kits for ALIRT members

Who is represented on the ALIRT Committee?

Livestock Producers (Arizona Cattlemen’s Association)

Arizona State Department of Agriculture (Arizona State Veterinarian)

University of ArizonaCooperative ExtensionDepartment of Animal ScienceDepartment of Veterinary Science and Microbiology

Arizona Veterinary Diagnostic LaboratoryUSDA

Who is represented on ALIRT First Responders?

Producers

ALIRT Veterinarians

ALIRT Extension Agents

State Livestock Officers

Who may also be involved in an ALIRT response?

State and Federal Veterinarians

Local/State and Federal Law Enforcement

Veterinarians and Scientists from the University of Arizona

Range, soil, water, wildlife and public health experts

ALIRTFIELD MANUAL

ALIRT RESPONSEHOW A RESPONSE IS STARTED

What starts an ALIRT response?

Identification of an unusual LIVESTOCK health eventResponse is limited to livestock (including ranch horses)Response is not available for single animal health issuesResponse is not available for poor reproductive performanceAbortion “storms” maybe included

Contact ALIRT by telephone

Contact to ALIRT may be done by:ProducerState Livestock OfficerCooperative ExtensionLocal veterinarian

How is ALIRT contacted?

ALIRT TELEPHONE NUMBERS:

STATE VETERINARIANS OFFICE TOLL FREE1-888-742-5334

ARIZONA VETERINARY DIAGNOSTIC LABORATORY1-520-621-2356

What information from the Producer is need for the ALIRT Committee?

Type and number of livestock involved

Type of symptoms livestock have

How long have symptoms been seen

Number and type dead and/or dying livestock

Herd History last 30 days:Herd movementSupplements

type/amount/how fedProcessing

vaccinationspray applicationparasite controlcastrationbranding

Pasture fertilizationirrigation

Recent weather eventsfrostrainlighting

Location and directions to animals or to contact point

Contact (call back) numbers for ALIRT Committee

ALIRT Committee and response

After initial contact to ALIRT the ALIRT Committee will hold a conference call

Conference call will determine type and extent of ALIRT response

Representatives of ALIRT Committee will:

Contact producer directlyObtain as much information as possible regarding incidentInsure producer is requesting an ALIRT response

Inform the producer as to the type and extent of ALIRT response

Develop contact point and time for ALIRT responders

Contact local/regional ALIRT team members

Establish contact point for ALIRT team and producer

ALIRT RESPONSEFIELD ACTIVITIES

What are the steps in an ALIRT Field Investigation?

1) ALIRT Team and Producer arrive on site and make preliminary assessment:

Does the site have any possible human safety issues?Should site be sealed off?Should personal protective equipment be used?

Is there any indication of criminal or intentional activity?Should local law enforcement be contacted?

Is there any indiction of a Foreign Animal DiseaseContact State Veterinarian and/or USDA APHIS AVIC

2) Establish incident control areaEstablish vehicle parking/control areaEstablish sample collection/processing siteRestrict livestock movement out of area

3) ALIRT Team and Producer examine and collect samples from surviving animals and/oranimals showing clinical signs

ALIRT Team collect and process samples following guidelines

4) ALIRT Team examine and collect samples from dead/dying livestockCollect and process samples following ALIRT guidelines

5) ALIRT Team collect and process other samples as indicatedPlant/ water/ feedProcess following ALIRT guidelines

6) Transport of dead/dying livestock to AzVDL for testing if indicated and approved

7) Euthanasia and disposal of dying/dead livestock if indicated and approved

8) Complete ALIRT Field Investigation Data Sheet

9) Maintain contact with ALIRT program (case supervisor)

10) Process and distribute digital images

11) Insure correct processing and shipment of all samples

ALIRT Incident Flow Chart

Testing/Euthanasia/DisposalDisposal

FAD/REGULATORY DISEASEAzDOA and/or USDA APHIS assume controlALIRT may provide assistance as requested

Treatment (Owner)Further Diagnostics/Consultants ALIRT

DisposalAfter Action Assesment

Communication ALIRTAz Producers/State/Federal/ Native American/Mexico

Agency Interactions:USDA/BLM/Wildlife/Public Health

PRIMARY ALIRT RESPONSEINFECTIOUS DISEASE/TOXICITY/PHYSICAL INJURY

Evidence CollectionTreatment/Disposal

Expert Witness

CRIMINAL ACTIVITYLocal/State/Federal Law Enforcement

(ALIRT)

ALIRT Field Response Veterinarians/SLO/Extension

Collection/Processing/Shipment SamplesPreliminary Diagnosis

Conference Call ALIRT CommitteeDetermination of ALIRT Response

Contact Producer

Contact ALIRT. ProgramAzDOA/AzVDL

Discovery of Livestock IncidentProducer/SLO/Extension/Local veterinarian

ALIRTFIELD MANUAL

FIELD INVESTIGATION DATA SHEET

CONTACT PRODUCER

RECORD TIME AND GPS LOCATION

INITIAL SITE ASSESSMENT:

SECURE CONTROL AREA

HUMAN SAFETY ISSUES

CRIMINAL ACTIVITY

POSSIBLE FAD


HISTORY

TYPE AND NUMBER LIVESTOCK INVOLVED

SYMPTOMS

DURATION

PROGRESSION

NUMBER AND TYPE LIVESTOCK ILL

DURATION

PROGRESSION

NUMBER AND TYPE OF LIVESTOCK DEAD

HERD HISTORYFORAGE

MOVEMENT LAST 30 DAYS

PASTURE FERTILIZATION/IRRIGATION

WEATHER EVENTS

SAMPLE COLLECTED

WATERNEW/CHANGED WATER SOURCE

OBSERVED CHANGES IN WATER QUALITY/APPEARANCE

SAMPLE COLLECTED

SUPPLEMENTSTYPE

AMOUNT

HOW FED

SAMPLE COLLECTED

PROCESSINGVACCINATION

SPRAY/TOPICAL APPLICATION

PARASITE CONTROL

SAMPLE COLLECTED

CASTRATION

OTHER PROCESSING

LIVE ANIMAL EXAMINATION SAMPLING

PHYSICAL EXAM

SAMPLE COLLECTED

DEAD ANIMAL EXAMINATION

FRESH TISSUES COLLECTED

FIXED TISSUES COLLECTED

OTHER HISTORYANY SIGNS/SYMPTOMS OBSERVED BY PRODUCER/FAMILY

ANY SIGNS/SYMPTOMS OBSERVED IN OTHER SPECIES

ANY SIGNS/SYMPTOMS OBSERVED IN WILDLIFE

RECENT ISSUES/CONFRONTATIONS/THREATS NEIGHBORS/ACTIVISTGROUPS

UNUSUAL INDIVIDUALS/VEHICLES/ACTIVITY

SAMPLE PROCESSING

SAMPLES CORRECTLY IDENTIFIED AND PROCESSED

SAMPLES CORRECTLY PREPARED FOR SHIPMENT

TRANSPORT SAMPLES TO DESIGNATED SHIPPING SITE

CONTACT SHIPPING AGENCY

CONTACT AZ VLD

ALIRTFIELD MANUAL

PROCEDURES FOR POSSIBLEFOREIGN ANIMAL DISEASE

1) List of Reportable Diseases:

See attached list of Reportable Diseases (pages 15-18)

See attached Mandatory Disease Reporting Form (page 19)

Further information on FAD’s can be found in ALIRT FIRST RESPONDERSMANUAL PAGES 139 TO 153

2) If a Reportable Disease is suspected contact:

Arizona Department of Agriculture 602-542-4293orVS Area Office AZ 480-491-1002

3) Secure site to prevent movement of personnel/vehicles/livestock into and out of area

4) If personnel and vehicles are forced to move out of containment area insure proper cleaningand disinfection:

SEE ALIRT FIELD KIT EQUIPMENT LIST

SEE CLEANING AND DISINFECTION INFORMATION IN FIRST RESPONDERSPROCEDURES GUIDE

5) Establish contact with ALIRT Committee

Arizona Department of AgricultureLivestock and Poultry Diseases for IMMEDIATE Reporting to the

State Veterinarian

(602) 542-4293FAX (602) 542-4290

Arizona Administrative Code R3-2-402, ‘Mandatory Disease Reporting by Veterinarians andVeterinary Laboratories’ - requires that all veterinarians and laboratories performing diagnosticservices on animals notify the State Veterinarian at (602) 542-4293 within 4 hours ofdiagnosing or suspecting any of these diseases.

DISEASE Listed asFAD by USDA

FADD mustinvestigate

EMERGINGAND EXOTIC

DISEASESOF ANIMALS

(page)

FOREIGNANIMALDISEASE

USAHA 1998EDITION

(page)

ALIRTPOSTER2005

LIVESTOCKCOMMONLYAFFECTED

African HorseSickness

yes 81 41 yes HORSE

African SwineFever

yes 82 52 yes SWINE

Anthrax no 87 yes CATTLE, SHEEP,GOATS, HORSES

Aujesky’s Disease(Pseudorabies)

no noSWINE, DOGS

Avian Influenza-Highly Pathogenic

yes 90 71 yes

Babesiosis(B. bovis, B.bigemina)

yes 98 81 no

Bluetongue no 92 yes

Bovine SpongiformEncephalopathy

yes 101 129 yes CATTLE

BovineTuberculosis

no 103 CATTLE



EMERGINGAND EXOTIC

DISEASESOF ANIMALS

(page)


USAHA 1998EDITION

(page)

ALIRTPOSTER2005


Chronic WastingDisease ofCervidae

no no DEER, ELK

Classic SwineFever (HogCholera)

yes 109 273 no SWINE

Contagious BovinePleuropneumonia

yes 112 154 yes CATTLE

Contagious CaprinePleuropneumonia

yes 114 161 yes

Contagious EquineMetritis

yes 115 170 no

Dourine(Trypanosomaequiperdum)

yes 116 182 no

EnterovirusEncephalomyelitisof swine

no 118 no

Equine InfectiousAnemia

no 124 no

EquinePiroplasmosis(Babesiosis)

yes 125 no

Equine ViralEncephalomyelitis(Eastern, Western,Japanese,Venezuelan orWest Nile)

yes forJapanese andVenezuelan

122 Japanese283

Venezuelan406

West Nile190

yes HORSEPEOPLE

VEE AND WESTNILE

Equine ViralArteritis

no 126 no



EMERGINGAND EXOTIC

DISEASESOF ANIMALS

(page)


USAHA 1998EDITION

(page)

ALIRTPOSTER2005


Foot and MouthDisease

yes 128 213 yes CATTLE, SHEEP,SWINE

Fowl Typhoid(Salmonellagallinarum)

no 130 no

Glanders(Pseudomonasmallei)

yes 131 245 yes

Heartwater yes 132 253 yes CATTLE, SHEEP,GOATS

Horsepox yes no

InfectiousHematopoieticNecrosis of fish

138 no

Lumpy SkinDisease

yes 143 303 yes

Ovine Epididymitis(B. ovis)

no 105 yes

Paratuberculosis no 159 no

Pestes des PetitsRuminants

yes 160 344 yes

Porcine Brucellosis no 105 yes

Pullorum Disease(Salmonellapullorum)

no no

Q fever (Coxiellaburnetii)

no 164 yes

Rift Valley Fever yes 169 353 no CATTLE, SHEEP,GOATS, PEOPLE



EMERGINGAND EXOTIC

DISEASESOF ANIMALS

(page)


USAHA 1998EDITION

(page)

ALIRTPOSTER2005


Rinderpest yes 170 362 yes CATTLE, SHEEP,GOATS, PIGS

Scrapie no no

Screwworm yes 173 372 yes ANY LIVESTOCK

Spring Viremia ofCarp

no

Sheep Pox andGoat Pox

yes 175 yes

Surra(Trypanosomaevansi)

yes 178 no

Swine VesicularDisease

yes 180 392 yes

Theileriosis yes 181 no

Trypanosomiasis no 185 29 no

VesicularStomatitis

no 188 419 yes CATTLE, SHEEP,GOATS, PIGS,

HORSES, PEOPLE

Viral HemorrhagicSepticaemia of fish

189 424 no

References:Arizona Administrative Code-Title 3, Article 4, R3-2 -402Foreign Animal Diseases — 1998 edition published by US. Animal Health AssociationInternational Animal Health Code — 1999 edition published by the Office International des EpizootiesNational Animal Health Reporting System Training Manual — 1999ALIRT Poster Center for Food Security & Public Health 2005

MANDATORY DISEASE REPORTING FORM(For diseases requiring mandatory reporting to the Office of the State Veterinarian, per A.A.C. R3-2-402)

(Print all Information – Note: Premises =animal location)

Owner’s name: ____________________________ Premises address:_____________________________

O-Address: _______________________________ P-City:______________________________________

O-City, State, Zip: _________________________ P-State, Zip: _________________________________

Type of Operation: _________________________ P-County:___________________________________

Phone: __________________________________ P-Latitude:__________________________________

County: _________________________________ P-Longitude:_________________________________

Primary Species on Premises initiating complaint: _____________________________________________Primary Species on Premises if different from above: __________________________________________Number of animals showing lesions by species: (use separate page for additional animals or use comments lines)

Species: # Animals: # Sick Description/ID: Samples Submitted:1. ____________ __________ _________ ______________________________ ________________

2. ____________ __________ _________ ______________________________ ________________

3. ____________ __________ _________ ______________________________ ________________

4. ____________ __________ _________ _________________________ _____________

Number and type of other susceptible species on premises:_____________________________________

Private Practitioner: _______________________ Clinic Name:_________________________________

Phone: _________________________________ Cell Phone:__________________________________

Has practitioner collected samples? ____ Yes ____ No If yes, what type?________________________

What lab were samples sent to? _______________________________ Date shipped:_______________

Onset date: ________________________________ Follow-up Date:_____________________________

Comments:_____________________________________________________________________________

ALIRTFIELD MANUAL

CRIME SCENE PROCEDURES

1) Producer and/or ALIRT First Responders make the determination the livestock incident involvesintent to intentionally cause harm to producer or livestock

2) Insure personal safety of all onsite personnel

3) Contact local law enforcement:

Inform law enforcement you are involved in a declared ALIRT Response

The type/number of animals involved

Why criminal intent is suspected

Need for crime scene investigation unit

Directions and contact information

ALIRTFIELD MANUAL

SAMPLE COLLECTION LIVE ANIMAL

NOTE FOR ALL SAMPLE COLLECTION:ALIRT First RespondersRecord location of all sample collections by GPSIf crime scene start evidence log

(see ALIRT First Responders FIELD MANUAL)

BLOOD SERUM1 or 2 9ml Monovette Serum Z tubesallow to clotcentrifuge (1-2 hours post collection)decant serum into new Snap Top (Plastic serum tube)REFRIGERATE OR FREEZE AFTER CENTRIFUGATION

BLOOD WHOLE1 9ml Monovette Li-Heparin LH tubegently rotate to insure clot preventionREFRIGERATE DO NOT FREEZE

URINE50 ml urine cupFREEZE

FECES200 grams (½ pound) more is betterdouble wrap OB sleeverefrigerate CAN BE FROZEN

MILK100 ml (2-3 oz)urine cup or small whirl packrefrigerate

ALIRTFIELD MANUAL

SAMPLE COLLECTIONNECROPSY

INTRODUCTION

ALIRT First Responders must provide:Ice chestIce 10-15 lbs or several “blue ice”

ALIRT Necropsy Guide is presented in a step wise progression. It is recommended to follow thisoutline and collect samples in order presented. With two or more ALIRT First Responders on site theALIRT veterinarian can perform the necropsy and the second Responder can assist in reviewing steps inthe Necropsy Guide, collection of samples and sample processing.


1) Positioning of animal:Place body with RIGHT side UP

2) Remove right front and rear legs and skin without opening abdominal or thoracic cavity

3) Start with fresh tissue collection. Use clean instruments

ALIRTFIELD MANUAL


FRESH TISSUE SAMPLE COLLECTION

Abdominal Cavity:

1) Open the abdominal cavity from behind the ribs to the spinal cord and back to the pelvis

FRESH TISSUE COLLECTION ABDOMINAL CAVITY:

NOTE ALL FRESH TISSUES GO INTO SEPARATE WHIRL PACKS

DO NOT OPEN ANY ABDOMINAL ORGANS UNTIL FRESH TISSUES HAVE BEENCOLLECTED

LIVER5x5x5 cm (2x2x2 inch) section into Whirl Pack

KIDNEY3 cm (1 ½ inch) section of the caudal pole of right kidney into Whirl Pack

Thoracic Cavity:

1) Cut through diaphragm into thoracic cavity to expose lung

FRESH TISSUE COLLECTION THORACIC CAVITY

LUNGCut from ventral border holding with forceps5x5 cm (2x2 inches) into Whirl Pack

ALIRTFIELD MANUAL


FIXED TISSUE SAMPLE COLLECTION


Fixed tissue is collected in large Whirl Pack with 10% buffered formalinVolume of formalin to tissue should be 5 parts formalin to 1 part tissueMultiple tissue can go into single Whirl Pack

DO NOT EXCEED 5 PARTS FORMALIN TO 1 PART TISSUEDO NOT MIX SAMPLES FROM DIFFERENT ANIMALS IN FORMALIN

LIVER3x3x1 cm (1x1x½ inch) 3 samples from different lobes

KIDNEY1 cm (½ inch) thick slice from capsule to papilla

HEART BLOOD (if free heart blood present)SERUM (HEART BLOOD)1 or 2 9ml Monovette Serum Z tubesallow to clotcentrifuge (1-2 hours post collection)decant serum into new snap-top tuberefrigerate DO NOT FREEZE

BLOOD WHOLE 1 9ml Monovette Li-Heparin LH tubegently rotate to insure clot preventionrefrigerate DO NOT FREEZE

HEART1 cm (½ inch) Cross section through right and left ventricles

LUNG3x3x1 cm (1x1x½ inch) 4 samples

1 each right and left cranial/ventral and caudal/dorsal lobes

TRACHEA2 cm (3/4 inch) long section

Bronchial lymph nodeDIGESTIVE TRACT

MESENTERIC LYMPH NODE FIXED SAMPLEwhole node

SMALL INTESTINE FRESH SAMPLE10 cm (4 inch) length tied off both ends with cable tiesREFRIGERATE OR FREEZE

INTESTINE FIXED SAMPLE5 cm (2 inches) longOpen sample and remove digesta BEFORE placing in formalin

Sample:RumenAbomasumDuodenumJejunumIleumLarge intestine

RUMEN CONTENTS FRESH SAMPLE10 kg (5 lbs)sample multiple sites in rumendouble wrap in OB sleeverefrigerateNOTE: If cyanide or ammonia toxicity is suspected freeze some rumen contents as soon aspossible

URINE50 ml urine cupFREEZE

BODY FAT100 grams (1/4 pound) freshsmall whirl pack

BRAINsplit brain on midline½ frozen in single large Whirl Pack½ formalin fixed

large Whirl Pack SEPARATE FROM OTHER FIXED TISSUE

FECES200 grams (½ pound)

more is betterdouble wrap OB sleeverefrigerate CAN BE FROZENNOTE: If botulism is suspected particularly in HORSES:Collect multiple sites in large intestineEach sample site into separate OB sleeveREFRIGERATE OR FREEZE

EYEwhole eye into small whirl packor use syringe and needle to pull fluid from eyeball put fluid into snap-top tuberefrigerate as soon as possible CAN BE FROZEN

LIVER (TOXICOLOGY)100 grams (1/4 pound)single Whirl Packrefrigerate as soon as possible CAN BE FROZEN

BONEsingle long leg bonedouble wrap OB sleeve

ALIRTFIELD MANUAL

SAMPLE COLLECTIONWATER AND FORAGE

NOTE FOR ALL SAMPLE COLLECTION:ALIRT First Responders

Record location of collection by GPSIf crime scene start evidence log

WATER

STREAM/POND/LAKERinse Nalgene collection bottle with water to be sampled or open under waterfill collection bottle 2/3 full and capkeep cool or freeze

HYDRANTallow hydrant to run before collectionrinse Nalgene collection bottle with water to be sampledfill collection bottle 2/3 full and capkeep cool or freeze

BLUE GREEN ALGAEcollect second water sample in Nalgene collection bottleRinse Nalgene collection bottle with water to be sampled or open under wateradd buffered formalin volume of formalin should be 10% total volume of sample

HAY/FORAGE/PLANTS

Bale hay use core sampler or sample multiple sites from bales representing current feed

Field samples 8-10 locations sample 4 foot area at grazing height mix collected forage and take sample

Keep samples in paper bags/not plastic

Silage/green chop can be frozen

ALIRTFIELD MANUAL

SAMPLE COLLECTIONPLANT COLLECTION FOR IDENTIFICATION


Use plain white note book provided in ALIRT field kit

Press plant between pages of note book

DO NOT USE TAPE OR GLUE

Collect as much of plant as possible including root system

Record on page color of flowers if present

If plant is too large for page:section plant and place in separate pagesrecord in note book part of plant

Collect fruit separately and press in note book

At end of collection seal note book with rubber band

Allow to dry before shipment

ALIRTFIELD MANUAL

SAMPLE SHIPPING PREPARATION

Shipping containers have been provided in the ALIRT field kits. In addition University of ArizonaExtension Offices have additional shipping kits. If more shipping kits are needed contact:

Dr. S. Peder CuneoAzVDLoffice 520-621-2356 ext 19cell 520-349-5608fax 520-626-8696e-mail [email protected]

AzVLD Sample Preparation Guidelinesfrom Az VDL web page http://microvet.arizona.edu/AzVDL/index.shtml

NOTE:For Extension offices to ship ALIRT samples, an individual in the office must have shipping training. The training is:

Read the information on sample preparation and shippingThe Extension Agent must sign off that the individual has read the informationAn e-mail, fax or written memo should be sent documenting that this training has beencompleted

Send documentation to:Dr. S. Peder Cuneoc/o Az VDL 2831 North FreewayTucson, AZ 85705fax 520-626-8696e-mail [email protected]

Labeling: Each specimen must be labeled with pertinent information that should include animal number, herdnumber, lot number, animal name, source, body site, and identity of the specimen. This is especiallytrue when samples from multiple animals are submitted. Preserving of specimen: Multiple specimens (such as fresh tissues) from a field necropsy must be individually packaged(PRIMARY RECEPTACLE) to prevent cross contamination, then enclosed in a secondary leak proofcontainer with absorbent material before being placed in a leak proof outer container. Containers withscrew top lids are preferred packages for tissue and feces. Plastic vials with screw top lids for blood,serum, and fluid transport are preferred as the primary containers for these specimens. If protected frombreakage, single-use vacuum blood-collection tubes may also be satisfactory. Guarded culture swabsmay be shipped in original packaging that is placed in an appropriate primary receptacle. Formalin fixed tissues from an individual animal may be pooled in a single container unless it isclinically relevant to have findings for each reported separately. If possible, the specimens should be no

more than 0.5 cm thick (about the thickness of five dimes) to allow adequate fixation. Allow fixation fortwenty-four hours, then remove the formalin from the container and replace it with a formalinsoaked gauze square to keep the tissues moist. If the specimen needs to be kept cool (i.e., blood,serum, etc.), use shipping containers designed specifically for refrigerated specimens. Please enclose thesubmission form in a plastic baggie and place the submission form in the package between thePRIMARY RECEPTACLE and the SECONDARY PACKAGING.

AzVDL Shipping Guidelines

IATA PACKING INSTRUCTION 650General RequirementsDiagnostic specimens must be packed in good quality packaging, which must be strongenough to withstand the shocks and loadings normally encountered during transport,including trans-shipment between transport units and warehouses as well as anyremoval from a pallet or over pack for subsequent manual or mechanical handling.Packaging must be constructed and closed so as to prevent any loss of contents whenprepared for transport, which might be caused under normal conditions of transport, byvibration, or by changes in temperature, humidity or pressure.Primary receptacles must be packed in secondary packaging in such a way that, undernormal conditions of transport, they cannot break, be punctured or leak their contentsinto the secondary packaging. Secondary packaging must be secured in outerpackaging with suitable cushioning material. Any leakage of the contents must notsubstantially impair the protective properties of the cushioning material or of the outerpackaging.

Packages must be prepared as follows:(a) For Liquids:The primary receptacle(s) must be leak proof and must not contain more than 500 ml; There must be absorbent material placed between the primary receptacle and thesecondary packaging; if several fragile primary receptacles are placed in a singlesecondary packaging, they must be either individually wrapped or separated so as toprevent contact between them. The absorbent material, such as cotton wool, must be insufficient quantity to absorb the entire contents of the primary receptacles and theremust be a secondary packaging that must be leak proof. The primary receptacle or the secondary packaging must be capable of withstanding,without leakage, an internal pressure producing a pressure differential of not less than95 kPa in the range of -40°C to +55°C (-40°F to 130°F). The outer packaging must not contain more than 4 L. (b) For Solids:The primary receptacle(s) must be sift proof and must not contain more then 500 g. If several fragile primary receptacles are placed in a single secondary packaging, theymust be either individually wrapped or separated so as to prevent contact between themand there must be a secondary packaging which must be leak proof. The outer packaging must not contain more than 4 kg. An itemized list of contents must be enclosed between the secondary packaging andthe outer packaging. Each completed package must be capable of successfully passing the drop test

described in 6.5.1 except that the height of the drop must not be less than 1.2 m. Packages consigned as freight must be at least 100 mm (4 in) in the smallest overallexternal dimension. Each package and the "Nature and Quantity of Goods" box of the air waybill mustshow the text "DIAGNOSTIC SPECIMEN PACKED IN COMPLIANCE WITH IATAPACKING INSTRUCTION 650". With effect from 1 January 2004, each package must also be marked in accordancewith 7.1.5.8 to indicate that the shipper has determined that the packaging meets theapplicable air transport requirements. The marking must be applied adjacent to thewords "Diagnostic Specimens". A Shipper's Declaration for Dangerous Goods is not required. Provided diagnosticspecimens are packed in accordance with this Packing Instruction, no otherrequirements of these Regulations apply except for the definition in 3.6.2.1.4 and thereporting of dangerous goods accidents and incidents in 9.6.1. Specific RequirementsAlthough exceptional cases, (for example, the shipment of whole organs) may requirespecial packaging, the great majority of diagnostic specimens can and must bepackaged according to the following guidelines.Substances shipped at ambient temperatures or higher: Primary receptacles includethose of glass, metal or plastic. Positive means of ensuring a leak-proof seal, such asheat seal, skirted stopper or metal crimp seal must be provided. If screw caps are used,these must be reinforced with tape. Substances shipped refrigerated or frozen (wet ice, prefrozen packs. Carbon dioxide,solid [dry ice]): Ice Carbon dioxide, solid (dry ice) or other refrigerant must be placed outside thesecondary packaging(s) or alternatively in an over pack with one or more completedpackages. Interior support must be provided to secure the secondary packaging(s) orpackages in the original position after the ice or Carbon dioxide, solid (dry ice) hasbeen dissipated. If ice is used the packaging must be leak-proof. If Carbon dioxide,solid (dry ice) is used the outer packaging must permit the release of carbon-dioxidegas.The primary receptacle must maintain its containment integrity at the temperature ofthe refrigerant as well as at the temperatures and pressure of air transport to which thereceptacle could be subjected if refrigeration were to be lost.Substances shipped in liquid nitrogen: Plastic capable of withstanding very lowtemperatures must be used instead of glass receptacles. Secondary packaging must alsowithstand very low temperatures and in most cases will need to be fitted over

Individual primary receptacles: If multiple primary receptacles are placed in a single secondary packaging, they mustbe separated and supported to ensure that contact between them is prevented.Requirements for shipment of liquid nitrogen must also be observed. The primaryreceptacle must maintain its containment integrity at the temperature of the refrigerantused as well as at the temperatures and pressure of air transport to which the receptaclecould be subjected if refrigeration were to be lost.Lyophilized substances: Primary receptacles must be either flame-sealed glassampoules or rubber-stoppered glass vials with metal seals.

ALIRTFIELD MANUAL

SAMPLE SHIPPING

1)After samples have been correctly processed and packaged for shipping they must be transported tothe Arizona Veterinary Diagnostic Laboratory for analysis. The AzVDL will be the site to send ALLdiagnostic samples collected during an ALIRT response. This is to insure that all samples are correctlyprocessed into the diagnostic system, are identified as ALIRT field samples and so all diagnosticinformation can be collected and reported.

Unless directed by State or Federal veterinarians all ALIRT samples must be sent to the AzVDL. Samples must be identified as ALIRT field samples.

2)To insure timely processing courier services have been provided to transport ALIRT samples to theAzVDL. Fees for the use of the courier services for transportation of ALIRT samples will be paid bythe ALIRT program. Non ALIRT samples going to the AzVDL may use these courier systems but thecost will not be covered by ALIRT.

3) The courier service used depends on location in Arizona:

South Central and South Eastern Arizona

OTR Courier ServiceP.O. Box 683

Green Valley, AZ 85622(800) 782-5172

Central, Western and Northern Arizona (below Strip)

K.C.S. Courier Service4494 W. Peoria Avenue, Suite 104

Glendale, AZ 853028:00 a.m. to 9:00 p.m.

(623) 915-3107

NOTE BOTH SERVICES DO NOT OPERATE ON WEEKENDS. FOR WEEKEND ALIRTSAMPLES CONTACT Az VLD DIRECTLY FOR SAMPLE PICK UP.

OTR Courier ServiceP.O. Box 683Green Valley, AZ 85622(800) 782-5172

Driver CUT-OFF Time=You must call ORT BEFORE this time to insure pickup in those citiesPICK-UP TIME= The time the drive will be leaving that particular cityOTR DRIVER CUT-OFF and PICK-UP TIME

CUT-OFF TIME PICK-UP TIMEWILCOX 3:00 PM 3:45 PMSAFFORD 2:00 PM 3:00 PMSIERRA VISTA 3:00 PM 3:15 PMFT HUACHUCA 12:00 PM 12:30 PMBISBEE 3:00 PM 3:30 PMTOMBSTONE 12:30 PM 1:00 PMDOUGLAS 2:30 PM 3:00 PMNOGALES 3:00 PM 3:30 – 5:00 PMPATAGONIA 2:00 PM 3:30 PMGREEN VALLEY 11:00 AM 11:30 – 12:00 SELLS 3:00 PM 3:30 PM

WILL PICK UP AT:

VETERINARY CLINICSCOCHISE COUNTY EXTENSION OFFICE450 S Haskell AvenueWILCOX, AZ 85643-2790(520) 384-3594

SANTA CRUZ COUNTY EXTENSION OFFICE3241 N Grand Avenue, Suite 6Nogales, AZ 85621-3917(520) 281-2994NO RANCH OR PRIVATE RESIDENCE PICK-UP AVAILABLE/ NO WHOLE ANIMALS

K.C.S. Courier Service4494 W. Peoria Avenue, Suite 104Glendale, AZ 853028:00 a.m. to 9:00 p.m.(623) 915-3107

For sample pick-up in the cities serviced by KC S., please call (623) 915-3107 before the arrival timeslisted on their driver itinerary page. When placing the call to K.C. S. Courier Service let them know that the pick up is for AZ VeterinaryDiagnostic Laboratory ALIRT

The driver will pick up at:Veterinary Clinic’sRanches within reasonable boundaries of driving route of the cites servicedExtension Office’s:

Coconino County Extension Office2304 N. 3rd StreetFlagstaff, AZ 86004-3605(928) 774-1868

Gila County Extension Office107 West FrontierSuite BPayson, AZ 85541(928) 474-4160

Apache County Extension Office845 West 4th NorthSt. Johns, AZ 85936(928) 337-2267 Extension Office’s:

Arrangements can also be made to meet at a pick-up point

KCS DRIVER TIME BY CITY

LAKE HAVASU-5:30 PM BULLHEAD CITY-5:00 PMPARKER-2:30 PM KINGMAN-3:00 PM & 6:00 PM QUARTSITE-7:30 PM FORT MOHAVE-5:30 PMSALOME-1:30 PM & 7:30 PM

YUMA-7:00 PM TUCSON-7:30 PMWELLTON-6:00 PM MARICOPA-5:00 PMGILA BEND-9:00 PM ELOY-6:00 PMBUCKEYE-10:00 PM MARANA-6:15 PMAJO-9:15 PM

GLOBE-5:00 PM FLORENCE-3:30 PMAPACHE JUNCTION-7:45 PM COOLIDGE-5:30 PMCOBRA VALLEY-6:00 PM HAYDEN-1:30 PMDUNCAN-2:00 PM KEARNEY-1:00 PMCLAYPOOL-6:15 PM MAMMOTH-1:45 PMGOLD CANYON-7:15 PM ORACLE-2:30 PMMIAMI-5:00 PM SAN MANUEL-2:00 PMQUEEN CREEK-8:00 PM SUPERIOR-12:30 PMSAFFORD-3:30 PM SACATION-6:15 PM

PAYSON/PINE-6:00 PM FLAGSTAFF-7:00 PMEAGER-5:00 PM COTTONWOOD-3:45 PMFT. MCDOWELL-4:30 PM CAMP VERDE-11:00 PMHEBER-6:45 PM SEDONA-5:15 PMOVERGAARD-6:30 PM OAK CREEK-6:00 PMST. JOHNS-3:00 PMPINETOP/LAKESIDE-2:00 PM TAYLOR-3:30 PMSTAR VALLEY-6:00 PMWHITE RIVER-3:00 PMWINSLOW-1:30 PMCIBECUE-2:00 PMHOLBROOK-2:30 PMSHOWLOW-5:00 PMSNOWFLAKE-3:15 PMSPRINGERVILLE-3:30 PMPRESCOTT-4:30 PM WICKENBURG-3:45 PMBLACK CANYON-7:30 PM SUN CITY-5:40 PMNEW RIVER-7:45 PM AVONDALE-7:00PMANTHAM-8:00 PM TOLLESON-7:30 PM

GOODYEAR-7:00 PM

PROCEDURES FOR USING THE GARMIN RINO® 120 GPSRECEIVER FOR ALIRT

It is very important for users to become comfortable with manipulating GPS unit settings andunderstanding their different functions. The quick start guide and manual that came with your unit willtake you through the basics.

GPS SetupThe following is information on how to setup your Garmin GPS for ALIRT data collection.

1) Press the power button and turn on the GPS unit.

2) Allow the GPS unit to acquire readings from satellites (youshould be outside and in an open area).

3) Press the page button on the left until you get to the MAINMENU page. This is for SETUP for TIME & UNITS

From the MAIN MENU - Select SETUP then TIME. Usingthe thumb stick make sure you have the following settings:

Time Format: 24 hourTime Zone: US-MountainDaylight Savings Time - NoWhen done click on the “X” in the upper right hand cornerto go back to the setup page

Select UNITS and confirm the following settings:Position format: UTM UPSMAP Datum: NAD83Distance Speed: StatuteElevation: Feet

Click X to return to the setup

Select SYSTEM. It should have WAAS Enabled

The most important settings to note are the position format and the MAP Datum. If you collectdata in another format be sure to make note of that format when submitting any data.

Basic UseOnce the receiver is set up there are two features that will be most useful for the ALIRT program:

saving your current position as a waypoint the “Find ’N Go” function.

To mark your current location and open the Mark Waypoint Page:marking a way point is simply capturing your current location and storing it in the unit’s memory. Theunit must be turned on and tracking satellites to mark a waypoint.

1. Press and hold the THUMB STICK IN until the shortcuts Menu opens.

2. Highlight ‘Mark Waypoint’ and press the THUMB STICK IN. The Mark Waypoint Page opens asshown below. You can also access this page by highlighting the Mark Icon on the Main Menu Pageand pressing the thumb stick in.

3 To save the waypoint, highlight ‘OK’ and press the THUMB STICK IN. If you do not want to save itas a new waypoint, press the PAGE key to cancel.

4. It is often easier in the field to keep the numbered waypoints and make note of the location, ratherthan renaming the points. However if you want to change the name of the waypoint, use the THUMB

STICK to highlight the waypoint name field, press the THUMB STICK INto activate the field and display the keyboard. Enter a new name, up to tem(10) characters long. When you have entered your new name, highlight‘OK’ and then press the THUMB STICK IN.

5. Record the data. It is a good idea to always have a hard copy backup ofthe waypoint data. You can use the data sheet at the end of thisdocument or one of your own. Be sure to record the Waypoint ID, thelocation coordinates: easting (the top numbers - 12 S 0608334) and thenorthing (the bottom numbers - 3572071), and note any commentsabout the location such as what was collected or what happened at that

location.

6. Highlight the ‘OK’ button at the bottom of the page and press the THUMB STICK IN to save thewaypoint.

To begin navigation to a waypoint using Find ‘N Go:

1. Press the THUMB STICK IN to display the Shortcuts Menu.

2. Highlight ‘Find ‘N Go’ and press the THUMB STICK IN.

3. Highlight ‘Waypoints’ and press the THUMB STICK IN. Select the desired option (by ‘Nearest’ orby ‘Name’), and press the THUMB STICK IN.

4. Highlight the desired waypoint and press the THUMB STICK IN.

5. With the Waypoint Page displayed, highlight the ‘Goto’ button at the bottom of the page and pressthe THUMB STICK IN to begin navigation. The navigation Page automatically opens to guide you toyour destination.

The two main components of the Navigation Page are the compass ring and the pointer. As you move, thecompass ring rotates showing your current direction of travel at the top of the display. The pointer pointstoward your destination. When the pointer is aligned with the vertical line at the top of the compass ring,you are on a direct course toward your destination. The unit has to be moving to navigate: If you arestanding still or moving slowly, the navigation data may not be accurate. You will find that when you getto the location, the arrow will keep changing directions and have you going in circles.

Final Note:When using another GPS model make sure you have the position format set to UTM UPS and the MAPDatum set to NAD83.

Information above was adapted from the Rino® 120 Quick Start Guide (Part Number 190-00418-01 Rev.A)

GPS DATA FIELDSHEET

GPS Make/Model: Garmin Rino 120 or other __________________________

Datum: NAD83 or other ________________________

Coordinate System: UTM Zone (circle) 11 or 12 Date: _____________________

Collected by: ____________________________________

Waypoint ID Easting Northing Description of location and of whatcollected at this point

ALIRT FIELD MANUAL


CONTACT PRODUCER



SECURE CONTROL AREA

HUMAN SAFETY ISSUES

CRIMINAL ACTIVITY

POSSIBLE FAD


HISTORY


SYMPTOMS

DURATION

PROGRESSION


DURATION

PROGRESSION


HERD HISTORYFORAGE



WEATHER EVENTS

SAMPLE COLLECTED



SAMPLE COLLECTED

SUPPLEMENTSTYPE

AMOUNT

HOW FED

SAMPLE COLLECTED



PARASITE CONTROL

SAMPLE COLLECTED

CASTRATION

OTHER PROCESSING


PHYSICAL EXAM

SAMPLE COLLECTED







RECENT ISSUES/CONFRONTATIONS/THREATS NEIGHBORS/ACTIVIST GROUPS


SAMPLE PROCESSING





CONTACT AZ VLD

ALIRT FIELD MANUAL


CONTACT PRODUCER



SECURE CONTROL AREA

HUMAN SAFETY ISSUES

CRIMINAL ACTIVITY

POSSIBLE FAD


HISTORY


SYMPTOMS

DURATION

PROGRESSION


DURATION

PROGRESSION


HERD HISTORYFORAGE



WEATHER EVENTS

SAMPLE COLLECTED



SAMPLE COLLECTED

SUPPLEMENTSTYPE

AMOUNT

HOW FED

SAMPLE COLLECTED



PARASITE CONTROL

SAMPLE COLLECTED

CASTRATION

OTHER PROCESSING


PHYSICAL EXAM

SAMPLE COLLECTED







RECENT ISSUES/CONFRONTATIONS/THREATS NEIGHBORS/ACTIVIST GROUPS


SAMPLE PROCESSING





CONTACT AZ VLD

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