NAME: KEVExam 2

Bubble in your answer sheet with the correct choice in each case (45 points)

1. On the graphs below, which line represents the addition of a NON-COMPETITIVEINHIBITOR to the original condition (line 1)?

2. Which statement is NOT true of the sequential model of allosteric binding?a. a subunit can exist in either tense or relaxed forms depending on the circumstancesb. binding of an inhibitor to one subunit makes binding of inhibitor to other subunitseasier. but does not fullv convert the other subunits to the tense form

-.A'

f c) binding of activator to one subunit makes binding of activator to other subunits easier,Yut does not affect substrate binding in any of the units

d. activator and substrate binding may be said to oostabilize" the relaxed forme. inhibitor binding may be said to oostabilize" the tense form

3. Which of the following is most likely to be phosphorylated?

ALys (R: (CHz)NHr*)(b)Ser (R : CH2OH)E cly (R: H)

4. Which is an saturated fatty acid?a. oieic acid: 18:l-Aeb. linoleic acid; 18:2-Ae'12c. linolenic acid; 18:3-Ae' 12' 15

d. Ala (R: CH3)e. they are all equally likely to bephosphorylated

d. arachidonic acid; 20:4-A5'8' rr' 14

6) norr. of the above\J

5. Which vitamin is biosynthesized from cholestelol via UV light?a. vitamin A @vitamin Db. vitamin B e. vitamin Ec. vitamin C

6. Which is an example of primary active transport?a. COzmoves across the membrane out of a cellb. Oz moves across the membrane into a cellc. glucose moves with the assistance of the membrane protein permease into a cell([glu]"* > [glu]'")d. glucose is co-transported into a cell via a Na-/glu symporter ([glu]*t < [glu]in, and([Na-]ou, > [Na-]in)

@Nu* is transported out of a cell via Na*/K*-ATPase

7. Which of the diagrams below represents D-glucose?

a. cHo b. qHo C gHo d. cHo e. cHo| | - I - i - |H-oH Ho-tH HtoH H--Fon H-1-oH

n-- | - -onn-for l r ,#tI I

Hotn Ho-l-r-r Hc I

n*on no*n n-*-oH no*n H-J-oH

n** no*n r-*-or ,-J-on no*"l l l l lCH2OH CH2OH CH2OH CHrOH CH2OH

8. Which of the following activates phosphorylase kinase?a. cAMP

- @ protein kinase

b, glycogen phosphorylase e. UDP-glucosec. glycogen synthase

9. In the disaccharide cx-lactose (glycosidic linkage is galactose-B-1,4-glucose), which ofthe followin g is ll1ftl trae?a" the galactose is in the o position d. neither monosaccharide can

@m. glucose is in the cr position muiargtatg

i. the anomeric in the cr position is e- both monosaccharides can mutarotate

locked into place

10. Which species is the electon donor in the reaction shown:NAD* + CH:CHzOH -r NADH + H* + CH3CHOa. CH:CHOb. NADH

d. NAD*e. insufficient information

@urcHroH

BCH 361 Hendrickson

11. Which general statement is TRUE regarding metabolism?a. catabolism is the sum of metabolic and anabolic reactionsb. anabolism is generally a reductive process which releases energyc. catabolism is generally a reductive process which requires energy

@catabolism is generally an oxidative process which releases energye. anabolism is generally an oxidative process which requires energy

12. For a system at equilibrium, which of the following MUST be true?a.AG>0b.aG<0

@lc: o

a. fructose-6-phosphate(6) glucose-6-phosphatei phosphoenolpyruvate

b. inhibited by citratec. inhibited by ATP

d. AGo'= 0e. AG"' < 0

O "ll ofthese are accurate

13. The formation of which intermediate occurs with a large, negative AG in glycolysis?d, 3-phosphoglyceratee. glyceraldehyde-3-phosphate

14. Which is NOT a product of the net reaction of glycolysis?a. 2ATP @itu"tut"b. 2 NADH Y ail of these are productsc. 2 pyruvate

15. Which does NOT a type of regulation of phosphofructokinase?a. activation by fructose-6-phosphate d. activated by AMP

Answer.the questions below; show calculations where relevant for partial credit1. (10 points) Given galactose (below), draw c-lactose (galactose-B-1,4-glucose).

BCH 361 Hendrickson

2. (15 points) Given the Lineweaver-Burk plot shown below, calculate Km and Vmaxfor each condition. Indicate whether the inhibitor is competitive or non-competitive.Sketch a Michaelis-Menton plot that corresponds to the data. (Note: your MM plot doesnot need to include numbers; simply indicate Km and Vmax, and get the basic shape ofthe plot correct). Label all axes.

o(t G---

Vn r.* '

K^

lN lhg

Vuar = 8. 11 xlo-L

Lr^ ' - l / -s.st =

( ,01,

: 1.7L

t/y-,nt --

- l / . - ' t =

q)

x to-L ( ,u0,? A

l. 81x lo -r (o -tt t \

ffrt tou.

V

o?-ro

K^D^S)

K^(t"t",5)

Inhibitor

ntercept -- -5.31

8, lq r to - '

- ',/,o,Lq

I cr*Pennvt

fsl

1rul".rb

BCH 361 Hendrickson

3. (15 points) ATP can be used to phosphorylate glucose to make glucose-6-phosphate.What is the net reaction? What is AGo' for the reaction, glven the following:

glucose + Pi + glucose-6-phosphate, AGo'= 13.8 kJ/molADP + Pi + ATP, AGo':30.5 kJ/molR=8.314JlmolK.T=298K

AT? t SLr,tcos4 >

bGl rrn,' l3.t .5l*l - 3o:5 LS/,r,toL ' -16'+ lc1l"+ol

' . i ; t : " r ' o;* ;*

BCH 361 Hendrickson

4. (15 points) Fill in the blank intermediates below.ADDITIONALLY:

l) Provide the names of the three major enzrymes, and clearly indicate which stepthey catalyze

2) Indicate the step(s) where ATP is required, and how many ATP are required PERGLUCOSE

3; Indicate the step(s) where ATP is produced, and how many ATP are producedPER GLUCOSE

4) Indicate the step(s) where NADH is produced, and how many NADH areproduced PER GLUCOSE

Lrrt,,)

[tr*>t

FaPAtP -- rnNP / i prn

AT )

gluc.'sc

PN / I Vvroutnftoa-

f;("P

lhrctose- I .6-bisphosphatc

/ \

bHrflp -----+ grrccrartrcrrrdc-.1-ph.r'h.rr. (

NA,S*

I L> ^JAs\1Y

| \. A,5? GlYcua{+-\ '3'btsPho.S '

lal*t^) ) |v v Pi l ) r ' +-phosplxrg lvccratc

I!-phosplroglr ccrrrtc

t

I-

phosphocnolpr 'rur 'atc

trt*A Hl i pu. Linnr*ePYnrvruK

Gr*,-)