electrochemical detection in hplc
DESCRIPTION
Electrochemical Detection in HPLC. ALEXYS ™ - HPLC/ECD. Applications Neuroscience Pharma Clinical analysis Food & Beverages Environmental. Why ECD?. extremely sensitive highly selective . Principle of HPLC/ECD. electrochemical reaction detector potential (E) is driving force. - PowerPoint PPT PresentationTRANSCRIPT
Electrochemical Detection in HPLC
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ALEXYS™ - HPLC/ECD
ApplicationsNeurosciencePharmaClinical analysisFood & BeveragesEnvironmental
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Why ECD?
extremely sensitivehighly selective
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Principle of HPLC/ECDelectrochemical reaction detectorpotential (E) is driving force
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Electrochemical reaction
ΔE
diffusion electron transfer reaction
Facilitates the EC reaction
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Role of electrode potential E
0
20
40
60
80
100
0 400 800 1200 1600
E (mV)
Sig
nal (
%)
no reaction
oxidation occurs
A B
Working range potential E
E working range determined by mobile phase
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Peak height vs. concentration
UV Beer’s law E = d C
ECD Cottrell equation ilim= 1.47 n F (Fm )1/3 (DA/b)2/3 C
A= WE area Ab = spacer thicknessC = concentrationilim= diffusion limited current measured (peak height)
peak height (E) vs concentration (C)
peak height (Ilim) vs concentration (C)
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Peak height vs. concentration
UV Beer’s law E = d C
ECD Cottrell equation ilim= f (A/b)2/3 C
A= WE area Ab = spacer thicknessC = concentrationilim= peak height
as simple as abc…
Electroactive Groups
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Application areas…
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www.myAntec.com
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Summary
Electrochemical Detection for HPLC
Potential drivenSelectiveSensitive
Thank you!