dr. abdul rehman poultry research institute, rawalpindi
TRANSCRIPT
New castle disease outbreak in poultry and its control
Dr. Abdul Rehman
Poultry Research Institute, Rawalpindi
Newcastle diseaseFirst identified in Newcastle in 1926Affects all species of birdsMortality up to 100% in chickensWidespread in Asia, Africa, Europe and South
AmericaIn USA, sporadic Newcastle disease (ND) occur
due to importation of infected birds. In 2002 ND outbreak in California, four million
birds were depopulated and cost the U.S. billions of dollars in damage and lost trade.
In Pakistan V-virulent ND appeared in Nov,2011
Clinical History (NDV) Broilers:
Highest economic losses Affected age is 25-35 days, also observed at 8-14
days Layers:
Maximum losses in rearing age, Also caused high mortalities & production losses in
laying birds Broiler breeders: same as in layers Fancy birds:
High mortalities in peacocks, pheasants and pigeons
Clinical signs Respiratory distress Greenish whitish diarrheaTorticollis Reduction in productionMortality up to 100%
Proventicular hemorrhages.
Caecal tonsils hemorrhages
EnteritisTracheitis
Postmortem lesions
ND Losses in BroilersMonth Mortality
(Mill)Av. Rate/Kg (Rs)
Value (Mill Rs.)
Nov,11 0.86 108.03 92.90
Dec,11 3.68 117.58 432.69
Jan,12 8.60 148.19 1274.40
Feb,12 15.48 151.31 2342.27
Mar,12 15.97 136.00 2171.92
TOTAL 44.59 6314.18
Population under report at PRI Million Month Broiler Layer Breeder Total
Nov,11 2.422 3.594 0.129 6.145
Dec,11 2.312 2.872 0.186 5.369
Jan,12 2.069 2.689 0.239 4.997
Feb,12 2.823 2.570 0.208 5.601
Mar,12 2.739 2.473 0.217 5.233
ND Incidence In Punjab
Newcastle disease virus
Genus Avulavirusin family ParamyxoviridaeThe genome is a single-stranded negative-sense
RNA consisting of 15,186 nucleotidesThe genome contains six genes in the order of 3’-
NP-P-M-F-HN-L-5’The virus is enveloped, roughly spherical, with a
diameter around 100-500nm.F and HN proteins form the external envelope
spikes
NDV isolates
Based on severity of disease, NDV isolates are grouped into three pathotypes:
–Lentogenic strainsCause mild or in-apparent respiratory disease
–Mesogenic strainsCause respiratory or nervous signs with
moderate mortality–Velogenic strains
Cause severe intestinal and/or neurologic disease resulting in high mortality –Neurotropic –Viscerotropic
Biological characterization of virus
Isolation & identification of virus Characterization of virus
Mean death time (less than 50 hrs)ICPI (more than 1.5) This indicates it’s a velogenic New castle disease virus Sequencing of M, HN, F genes F gene nucleotide similarity 97 to 100% M gene nucleotide similarity 98.5 to 100% HN gene nucleotide similarity 99.2 to 100%This indicates mutation and close relation with linage
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Contributing factorsIndiscriminate vaccinationClose proximity of farmImproper biosecurity/ managementImproper disposal of dead birdsMorbid Bird’s marketingInsufficient disease diagnostic facilities
ND Surveillance at PRI(Nov,11 to date)
Total no. of sera samples 18847Total no. of tracheal swab 8646Total no. of cloacal swab 20175Total no. of tissue sample 2350Total sample collected
50018
Sample processing Embryo inoculation Virus isolation HAHIRT-PCR
Results (NDV isolated)
Broilers flocks 33Layer flocks 22Rural flocks 12Broiler breeder flocks 05Fancy bird flocks 07Total isolates 79
Research trial on different vaccine regimes Lasota, Mukteswar (live, killed), Velogenic
killed (oil based) were employedMukteswar killed (oil based) and Velogenic
killed (oil based) vaccines prepared in disease section PRI, Rawalpindi
7 groups was made 6 experimental and one unvaccinated control
Experimental Vaccination schedule Group
No. Day of vaccination
Day 1 Day 10 Day 21 Day 28
1 ND+IB (E/D) Lasota (E/D) Lasota (D/W) Velogenic field strain challenge 2 ND+IB (E/D) Mukteswer Live
(E/D)Lasota (D/W)
3 Mukteswer Killed injection + ND+IB (E/D)
Mukteswer Live (E/D)
Lasota (D/W)
4 Velogenic Killed injection + ND+IB (E/D)
Mukteswer Live (E/D)
Lasota (D/W)
5 ND+IB (E/D) Mukteswer Killed injection + Lasota (E/D)
Lasota (D/W)
6 ND+IB (E/D) Velogenic Killed injection + Lasota (E/D)
Lasota (D/W)
7 No vaccine No vaccine No vaccine
HI titers log2Group No. Age in days
Day 1 Day 9 Day 20 Day 27
Group 1 6.2 3.3 3.4 5.0
Group 2 5.5 3.2 4.4 4.7
Group 3 7.3 3.6 3.2 5.5
Group 4 5.4 2.7 3.7 5.9
Group 5 6.3 4.5 4.4 6.2
Group 6 6.2 3.2 3.5 5.6
Group 7 6.6 3.4 2.0 2.2
Protection after experimental challenge Group No. Total no. of
birds at 28 days
Mortality with in 8 days
Total birds survived
% protection
1 43 10 33 76.74
2 42 09 33 78.57
3 43 7 36 83.72
4 42 7 35 83.33
5 43 7 36 83.72
6 42 3 39 92.85
7 42 41 1 2.3
Results Velogenic killed vaccine at 10th day along with
live lasota vaccine gave maximum protection to 92.85 %
Velogenic killed vaccine at day 1st not proved so well as day 10th
Mukteswer killed vaccine at day 10th also gave significant protection 83.72 %
Mukteswer killed vaccine at day first did not prove as much protective
Mukteswer killed vaccine proved well as compared to live vaccine
Conclusion Homologous vaccine gave better result as
compared to Mukteswer and Lasota vaccinesHomologous vaccine may also reduce virus
shedding during infectionHomologous vaccine along with eradication
program may be helpful in the control of current outbreak
Research findings of various organizationsUVAS
Repeatedly isolated and confirmed presence of ND virus in diseased birds
Killed vaccine prepared from local isolate gives almost 100% protections against the challenge from the field virus
NRLPD Virus is closely related to the Malaysian ND virus isolate Protection study showed that clone killed vaccine
prepared from local isolate gave better protectionVRI
Live and Killed vaccine prepared from Mukteswer proved helpful
RecommendationsStrict biosecurity rules must be implementedProper disposal of dead birdsProper dis-infection between the flocksProper litter disposalIt was unanimous opinion that killed vaccine must be included
in the vaccine scheduleRepeated vaccine should be discouraged (Too many)Vaccine prepared from local isolate should only be used for
experimental purposes and should not be used at commercial level
More study work needs to be done to find out the solution to this problem
Regulatory control in poultry sector Maintain farms proximity Biosecurity/ management Sale/ Disposal of birds
Augment Poultry disease diagnostic facilities
Thanks