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Principles of PCR
• Principles • Design of primers • PCR for cloning • Real 3me PCR • Error frequency • Tips and tricks
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Fig 9.1 p 148
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Fig 9.2 p 149
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Fig 9.3, 9.4 p 149
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Fig 9.5 p 150
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Fig 9.6 p 150
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Fig 9.7 p 152
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Fig 9.8 p 153
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Fig 9.9 p 154
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Fig 9.10 p 155
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Fig 9.11 p 155
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Fig 9.12, 9.13 p 156
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Fig 9.14 p 157
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Fig 9.15 p 157
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Fig 9.16 p 158
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Fig 9.18, p 160
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Fig 9.19 p 160
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Fig 9.20 p 161
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Tips and tricks
• Make a master mix solu3on (i.e. mix buffers, water, dNTP:s) enough for all samples plus one
• Use the protocol recommended by the manufacturer
• Use fresh solu3ons • Avoid contamina3on • Are there any possible inhibitors in your sample? Increase volume of added polymerase