Methods in Enzymology
Volume 125
BiomembranesPart M
Transport in Bacteria, Mitochondria, an dChloroplasts :
General Approaches and Transport Systems
Sidney FleischerBecca Fleischer
CONTRIBUTORS TO VOLUME 125 X i
PREFACE i' .,1 XVi i
VOLUMES IN SERIES XIX
Section I . General Approache s
1 . Energetics of Proton Transport and Secondary HAGAI ROTTENBERG
3Transport
A. Membrane Characteristics and Chemistry
2 . Isolation of Highly Coupled Heart Mitochondria PETER P . TOTH ,in High Yield Using a Bacterial Collagenase
SHELAGH M . FERGUSON-MILLER ,AND CLARENCE H . SUELTER 1 6
3. Alkyl Glycoside Detergents : Synthesis and Appli- T . VANAKEN ,cations to the Study of Membrane Proteins
S . FOXALL-VANAKEN ,S . CASTLEMAN, AN DS . FERGUSON-MILLER
27
4 . Lipid Enrichment and Fusion of Mitochondrial In- CHARLES R . HACKENBROCKner Membranes
AND BRAD CHAZOTTE
3 5
5 . Changes of Surface and Membrane Potentials in NAOKI KAMO ANDBiomembranes
YoNOSUKE KOBATAKE
46
6 . Rate of Redox Reactions Related to Surface Po- SHIGERU ITOH ANDtential and Other Surface-Related Parameters in MITSUO NISHIMURA
58
Biological Membrane s
7 . Use of N,N'-Dicyclohexylcarbodiimide to Study MACIEJ J . NAfECZ ,Membrane-Bound Enzymes
ROBERT P . CASEY, AN DANGELO AzzI
86
8. Voltammetric Measurements of Quinones
ROGER C . PRINCE ,PAUL LLOYD-WILLIAMS ,J . MALCOLM BRUCE, AN DP . LESLIE DUTTON
109
9. Determination of Partition Coefficients of BARRY S . BRAUN ,Quinones by High-Performance Liquid Chro- ULI BENBOW ,matography
PAUL LLOYD-WILLIAMS ,J . MALCOLM BRUCE, ANDP . LESLIE DUTTON
119
B. Membrane Assembly, Mutations, and Cloning Strategy
10 . Function of Protonmotive Force in Translocation LINDA L . RANDALL
129of Protein across Membrane s
11 . Use of Secretion Cloning Vectors for Guiding the CHARLES A . LuNN ,
Localization of Proteins in Escherichia coli
MASAYASU TAKAHARA, AN D
MASAYORI INOUYE
13 8
12 . Use of lac Gene Fusions to Study Transport Pro- HOWARD A . SHUMAN
150tein s
13 . Use of Transposons to Isolate and Characterize MAURICE C . JONES-MORTIMER
Mutants Lacking Membrane Proteins, Illus- AND PETER J . F . HENDERSON 15 7trated by the Sugar Transport Systems of Es-
cherichia coli
14 . ECF Locus in Escherichia coli : Defect in Energi- JEN-SHIANG HONG
180zation for ATP Synthesis and Active Transport
15 . Mutations in the eup Locus of Escherichia coli, CHARLES A . PLATE
187Energy Uncoupled Phenotype
16 . Methods for Mutagenesis of the Bacterioopsin MARIE A . GILLES-GONZALEZ ,
Gene
NEIL R . HACKETT ,
SIMON J . JONES ,
11 .GOBIND KHORANA ,
DAE-SIL LEE, KIN-MING LO ,
AND JOHN M . MCCoY
190
17 . Oligonucleotide-Directed Site-Specific Mutagene- HEMANFA K . SARKAR ,
sis of the lac Permease of Escherichia coli
PAUL V . VIITANEN ,
ETANA PADAN ,
WILLIAM R . TRUMBLE ,
MOHINDAR S . POONIAN ,
WARREN MCCOMAS, AN D
H . RONALD KABACK
21 4
18 . DNA Sequence and Transcription of Genes for VICTOR L . J . TYBULEWICZ ,
ATP Synthase Subunits from Photosynthetic GUNNAR FALK, AN D
Bacteria
JOHN E . WALKER
230
Section II . Bacterial Transport
19. Membrane Transport in Rickettsiae
HERBERT H . WINKLER
25 3
20. Transport in Mycoplasmas
SHLOMO ROTTEM AN D
VINCENT P . CIRILLO
259
21 . Transport through the Outer Membrane of Bac- HIROSHI NIKAIDO
26 5
teria
22 . Binding Protein-Dependent Active Transport in CLEMENT E . FURLONG
27 9Escherichia coli and Salmonella typhimuriu m
23 . Calcium-Induced Permeabilization of the Outer JOHANN M . BRASS
28 9Membrane : A Method for Reconstitution ofPeriplasmic Binding Protein-Dependent Trans -port Systems in Escherichia coli and Salmo-nella typhimurium
24 . Reconstitution of Periplasmic Binding Protein-De- ARTHUR G . HUNT AN Dpendent Glutamine Transport in Vesicles
JEN-SHIANG HONG
302
25. Isolation and Crystallization of Bacterial Porin
R . MICHAEL GARAVITO AN D
JÜRG P . ROSENBUSCH
309
26. Ion Extrusion Systems in Escherichia coli
BARRY P . ROSEN
328
27. Intracellular pH Regulation in Bacterial Cells
ETANA PADAN AN D
SHIMON SCHULDINER
33 7
28 . Regulation of Internal pH in Acidophilic and Alka- T . A . KRULWICH AN D
lophilic Bacteria
A . A . GUFFANTI
35 2
29 . Peptide Transport in Bacteria
CHRISTOPHER F . HIGGIN S
AND MARGARET M . GIBSON 36 5
30 . Methods for the Study of the Melibiose Carrier of DOROTHY M . WILSON ,
Escherichia coli
TOMOFUSA TSUCHIYA, AN D
T . HASTINGS WILSON
37 7
31 . Assay, Genetics, Proteins, and Reconstitution of PETER J . F. HENDERSON AN DProton-Linked Galactose, Arabinose, and Xy- ANDREW J . S . MACPHERSON 387lose Transport Systems of Escherichia coli
32. Purification, Reconstitution, and Characterization PAUL VHTANEN ,
of the lac Permease of Escherichia coli
MICHAEL J . NEWMAN ,
DAVID L . FOSTER ,
T . HASTINGS WILSON, AND
H . RONALD KABACK
429
33. Preparation of Monoclonal Antibodies and Site- NANCY CARRASCO ,
Directed Polyclonal Antibodies against the lac DORIS HERZLINGER ,
Permease of Escherichia coli
WALEED DANHO, AN D
H . RONALD KABACK
45 3
34. Glycerol Facilitator in Escherichia coli
E . C . C . LIN
46 7
35. Polyhydric Alcohol Transport by Bacteria
JOSEPH W . LENGELER
47 3
36. Alanine Carrier from Thermophilic Bacteria
H . HIRATA
48 5
37 . Generation of a Protonmotive Force in Anaerobic BART TEN BRINK AN D
Bacteria by End-Product Efflux
WIL N . KONINGS
49 2
38 . Light-Driven Chloride Transport in Halorhodop- BRIGITTE SCHOBERT AN Dsin-Containing Cell Envelope Vesicles
JANOS K . LANYI
510
39 . Sodium Translocation by NADH Oxidase of Vi- HAJIME TOKUDA
520brio alginolyticus : Isolation and Characteriza-
tion of the Sodium Pump-Defective Mutant s
40 . Preparation, Characterization, and Reconstitution PETER DIMROTH
53 0of Oxaloacetate Decarboxylase from Klebsiell aaerogenes, a Sodium Pump
41 . Sodium Pump Methylmalonyl-CoA Decarboxyl- WILHELM HILPERT AN D
ase from Veillonella alcalescens
PETER DIMROTH
540
42 . Biotin-Dependent Decarboxylases as Bacterial WOLFGANG BUCKEL
547Sodium Pumps : Purification and Reconstitution
of Glutaconyl-CoA Decarboxylase from Acid-aminococcus fermentans
43 . Anion Exchange in Bacteria : Reconstitution of SURESH V . AMBUDKAR AN D
Phosphate : Hexose 6-Phosphate Antiport from PETER C . MALONEY
55 8Streptococcus lactis
44. Proton-Driven Bacterial Flagellar Motor
ROBERT M. MACNAB
56 3
45 . Sodium-Driven Flagellar Motors of Alkalophilic YASUO IMAE ,
Bacillus
HIROSHI MATSUKURA, AND
SYOYU KOBAYASHI
582
Section III. Transport in Mitochondria and Chloroplast s
A. Mitochondria
46 . Mitochondrial Outer Membrane Channel (VDAC, CARMEN A . MANNELLA
59 5Porin) Two-Dimensional Crystals from Neu-rospora
47 . Reconstitution of ADP/ATP Translocase in Phos- REINHARD KRÄMER
61 0
pholipid Vesicles
48 . Fluorescent Nucleotide Analogs as Active Site MARTIN KLINGENBERG
61 8Probes for the ADP/ATP Carrier and the Un-
coupling Protei n
49 . Techniques for NMR and ESR Studies on the KLAUS BEYER AN D
ADP/ATP Carrier
ANTON MUNDING
63 1
50. Fluorescent Probes of the Mitochondrial ADP/ MARC R . BLOCK ,
ATP Carrier Protein
FRANcoIS BOULAY ,
GERARD BRANDOI .IN ,
YVES DUPONT ,
GUY J . M . LAUQUIN, AND
PIERRE V . VIGNAIS
639
51 . ADP/ATP Carrier : Analysis of Transmembrane WERNER BOGNER AN D
Folding Using Pyridoxal Phosphate
HEINRICH AQUILA
650
52. Chemical Modifications and Active Site Labeling MARC R . BLOCK ,
of the Mitochondrial ADP/ATP Carrier
FRANgOIS BOULAY ,
GERARD BRANDOLIN ,
GUY J . M . LAUQUIN, AN D
PIERRE V . VIGNAIS
65 8
53 . Measurement ofCitrate Transport in Tumor Mito- RONALD S . KAPLAN ,chondria
RISA A . PARLO, ANDPETER S . COLEMAN
67 1
54 . Partial Purification and Reconstitution of the Tri- FERDINANDO PALMIERI ,carboxylate Carrier from Rat Liver Mitochon- ITALO STIPANI ,dria
GIROLAMO PREZIOSO, AN D
REINHARD KRÄMER
69 2
55 . Isolation and Reconstitution of the Phosphate HARTMUT WOHLRAB ,Transport Protein from Mitochondria
HANNO V. J . KOLBE, AND
ANNE COLLINS
697
B. Chloroplast s56 . Transport of Metabolites across the Chloroplast H. W. HELDT AN D
Envelope
U . I . FLÜGGE
70 5
57 . Chloroplast Phosphate-Triose Phosphate-Phos- HA . FLÜGGE AN Dphoglycerate Translocator: Its Identification, H. W . HELDT
71 6Isolation, and Reconstitutio n
AUTHOR INDEX 73 1
SUBJECT INDEX 761