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Population Genetics
Emerged in the 1920s Founders: R.A. Fisher, Sewall Wright, J.B.S. Haldane Provided the mathematical foundations for the evolutionary process Also important in: e.g. Agriculture, medical and human genetics, bacterial resistance, evolution of virulence
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Population Genetics
What are the levels of genetic variation in populations? How do different mating patterns affect genotype and allele frequencies? What forces are responsible for changes in the genetic composition of populations? Mutation, Migration, Genetic drift, Natural selection
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Phenotype M MN N TOTAL Genotype MM MN NN Obs # 180 240 80 500 Genotype 180/500 240/500 80/500 proportions = 0.36 = 0.48 = 0.16
1. Using the numbers of various genotypes observed in the population p = freq(M) = # of M alleles/total alleles = (2 x 180 + 240)/ (2 x 500) = 0.6 q = freq(N) = # of N alleles/total alleles = (2 x 80 + 240)/ (2 x 500) = 0.4 Note that the alleles frequencies p + q = 1 or you've made a mistake. 2. Using the proportions of each genotype. p = freq (MM) + 1/2 x freq (MN) = 0.36 + 0.48/2 = 0.6 q = freq (NN) + 1/2 x freq (MN) = 0.16 + 0.48/2 = 0.4
Genotype and allele proportions for the MN blood group polymorphism
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Allozymes, gel electrophoresis and the “Find em and Grind em” era.
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Alcohol dehydrogenase in pollen of Asclepias syriaca (milkweed)
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Esterase in T. subulata
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Glucose phospohate isomerase in autotetraploid T. subulata
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Cleaved amplified polymorphism in style polygalacturonase
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Schematic of allozyme gel electrophoresis showing an enzyme segregating for 3 alleles, F, M, S, in a population sample.
F M S
SS MS MM FS FM FF MS MM FS FM SS SS
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See Fig 18-14 for view of SNP variation across a sample of humans. Note the different numbers of haplotypes.
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Tristyly in Oxalis. Plants exhibit negative assortative mating
short-styled mid-styled long-styled S- -- ssM- ssmm
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Allele frequency change due to mutation alone
00.10.20.30.40.50.60.70.80.9
1
0 20000 40000 60000 80000 100000
Generation
Freq
of A
alle
le
rate = 0.00001rate = 0.000001
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A
B
a
b*
A
B
a
B
Mutation B to b*
Mutation and the generation of linkage disequilibrium
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Decay of linkage disequibrium with r = 0.5
0
0.1
0.2
0.3
0.4
0.5
0.61 5 9 13 17 21 25 29 33 37 41 45 49
Generation
Two-
locu
s Fr
eque
ncy
ABAbaBab
Begin with complete linkage disequilbrium with just AB and ab combinations of alleles at equal frequency and allow random mating and recombination with r = 0.5. Eventually there is decay of linkage diseq and we end up with equal freqs of AB Ab aB ab in the population. In subsequent slides, small r-values reduce rate of decay
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Decay of linkage disequibrium with r = 0.1
0
0.1
0.2
0.3
0.4
0.5
0.61 5 9 13 17 21 25 29 33 37 41 45 49
Generation
Two-
locu
s Fr
eque
ncy
ABAbaBab
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Decay of linkage disequibrium with r = 0.01
0
0.1
0.2
0.3
0.4
0.5
0.61 5 9 13 17 21 25 29 33 37 41 45 49
Generation
Two-
locu
s Fr
eque
ncy
ABAbaBab
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Decay of linkage disequilibrium with r = 0.01
0
0.1
0.2
0.3
0.4
0.5
0.61 14 27 40 53 66 79 92 105
118
131
144
157
170
183
196
Generation
Two-
locu
s fre
quen
cy
ABAbaBab
So even with a 1cm distance between genes and 200 generations the disequilibrium has not yet fully decayed
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