Introducing the New TriPlus RSH Autosampler
August 21st, 2011
Introducing the new Triplus RSH Autosampler
You are Productive
Solving your Chromatography Challengesg y g p y g
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Triplus RSH at a glance
TriPlus RSH is an entirely new platform of GC and GC/MS autosamplers ThermoFisher Scientific has developed allowing:
Unattended, weekend long operationsUnattended, weekend long operations• Up to 648 2-mL vials and multiple 100 mL solvent bottles stations
Unattended, injection modes changes in a sequence: liquid Unattended, injection modes changes in a sequence: liquid –– HS HS -- SPMESPME• ATC (Automatic Tool change) Station for automatic recognition and syringe
exchanges during operation
Reliable, reproducible injections with microvolume samplesReliable, reproducible injections with microvolume samples• <2%RSD and up to 3 injections possible with 5 l sample volume in vial.
Advanced sample handling and sample preparationAdvanced sample handling and sample preparation• Dilution, derivatization, standard addition, internal standard additions, etc.
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Summary
• TriPlus RSH: four major benefits, four good reasons to buy it
T iPl RSH d t biliti• TriPlus RSH product capabilities
• TriPlus RSH Configurations• Clone Mode
• TriPlus RSH proof datap
• TriPlus RSH Software Integration
• TriPlus RSH Validated Consumables
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TriPlus RSH Autosampler: Integrated Sampling system
• TriPlus RSH (Robotic Sample Handling) is an entirely new platform of robotic autosampler
• Improved autosampler performance and li bilit f li id h d d SPMEreliability for liquids, headspace and SPME
• Accurate injection from microvolume samples
• No more users required when switching from liquid to Headspace or SPME
Runs sequences with multiple injection• Runs sequences with multiple injection modes, unattendedly changing the tool/syringe
• Sample Preparation such as Dilution, Internal standard addition, derivatization with robotic tool changing technology
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TriPlus RSH : four good reasons to buy it!
• Ultimate productivity for reliable 24/7 operations• More robotic X-Y-Z precise movement controls• The largest sample capacity for unattended sequences
• More vials can be loaded with larger solvent washing capability• Value for the investmentValue for the investment
• The highest configuration scalability• Full compatibility of all accessories with all configurations
• Substantially reducing $/sample lab costs• New unique capability to automate basic sample prep
• Dilution internal standard addition derivatization• Dilution, internal standard addition, derivatization• Even higher quality results and performance
• Full support of all Thermo injection capabilities
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TriPlus RSH : The highest precision
M b ti X Y Z i t t l• More robotic X-Y-Z precise movement controlsSolvent: Ethyl Acetate10 ul syringeLiquid taken from washing station and used to fill empty vialsTransfer per vial: 8 ul, 15 times
Typical repeatibility 0.066%
Solvent: waterSolvent: water100 ul syringeLiquid taken from washing station and used to fill empty vialsTransfer per vial: 100 ul, 1 time
Typical repeatibility 0.021%
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Indipendently from GC performance
TriPlus RSH : The highest precision
• More robotic X-Y-Z precise movement controls
Solvent: 2-propanol100 l i100 ul syringeLiquid taken from washing station and used to fill empty vialsVolume Linearity > 0.99999
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Indipendently from GC performance
TriPlus RSH : Ultimate productivity for reliable 24/7 operations
• The largest sample capacity, ensuring true, weekend-long operations
• Multiple tray holders: • Up to 648 2-mL vials
U t 840 0 7 0 5 L i i l• Up to 840 0.7- or 0.5-mL micro vials
• The largest solvent washing capabilityThe largest solvent washing capability• Four different 10-mL solvent vials to
maximize cleaning efficiency• Multiple 100-mL solvent bottles
stations for high sample throughput, with waste collected externally
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TriPlus RSH : four good reasons to buy it!
• Ultimate productivity for reliable 24/7 operations• More robotic X-Y-Z precise movement controls• The largest sample capacity
• More vials can be loaded with larger solvent washing capability• Value for the investmentValue for the investment
• The highest configuration scalability• Full compatibility of all accessories with all configurations
• Substantially reducing $/sample lab costs• New unique capability to automate basic sample prep
• Dilution internal standard addition derivatization• Dilution, internal standard addition, derivatization• Even higher quality results and performance
• Full support of all Thermo injection capabilities
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TriPlus RSH Autosampler: Value for the investment
B i Li id fi ti B i h d fi ti B i d fi tiBasic Liquid configuration Basic headspace configuration Basic duo configuration
With 162 2-mL vials capacity
With 60 10- or 20- mL vials capacity
With 60 10- or 20-mL and 162 2-mL vials capacitycapacity
Standard liquid syringe tool (10 L)
Standard washing
capacity
HS gas tight syringe tool (2.5 mL)
Agitation and Incubation
2 mL vials capacity
HS gas tight syringe tool (2.5 mL) and standard liquid syringe tool (10 L)Standard washing
station (5 10-mL vials)Agitation and Incubation oven, up to 6 10- or 20-mL vials up to 200°C
Agitation and Incubation oven, up to 6 10- or 20-mL vials up to 200°C
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Standard washing station (5 10-mL vials)
TriPlus RSH Autosampler: Value for the investmentBasic Liquid confBasic Liquid conf fully compatible with
Agitator and Heated f HS SPME
Peltier heating or cooling of samplesoven for HS, SPME or
sample prepcooling of samples
SPME fiber conditioning for SPME applications
More sample trays for higher capacity
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for SPME applications higher capacityVortexer for Intensive sample mix
Barcode reader for sample tracking
TriPlus RSH : four good reasons to buy it!
• Ultimate productivity for reliable 24/7 operations• More robotic X-Y-Z precise movement controls• The largest sample capacity
• More vials can be loaded with larger solvent washing capability• Value for the investmentValue for the investment
• The highest configuration scalability• Full compatibility of all accessories with all configurations
• Substantially reducing $/sample lab costs• New unique capability to automate basic sample prep
• Dilution internal standard addition derivatization• Dilution, internal standard addition, derivatization• Even higher quality results and performance
• Full support of all Thermo injection capabilities
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TriPlus RSH: substantially reducing $/sample lab costs
N A t ti T l Ch i (ATC) St tiNew Automatic Tool Changing (ATC) Station• Unique ability to automatically handle up to six different syringes
Unattended syringe exchange during a sequence
ATC Station
• Unattended syringe exchange during a sequence • Zero carryover and no compromise in sample preparation
ATC Station
TR (Tool Releasing) Station for manual
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syringe exchange
TriPlus RSH: substantially reducing $/sample lab costs
Automated basic sample handling and sample prep • Sequential dilution: dilute a commercial standard to
meet the analytical requirementmeet the analytical requirement
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Labor costs strongly reduced while improving accuracy
TriPlus RSH: Substantially Reducing $/sample Lab Cost
Automated basic sample handling and sample prep • Calibration dilution: automatically prepare calibration
points adding internal standardspoints adding internal standards
ISTD
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Labor costs strongly reduced while improving accuracy
Calibration Dilution
0.08 – 4 µg/mL 0.08 – 80 µg/mL
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R T : 1 1 .0 6 - 2 1 .0 4R T : 1 8 2 2 N L :1 3 5 TMB 1 2 3-TMB
Calibration Dilution
4 0
6 0
8 0
1 0 0R T : 1 8 .2 2
R T : 1 9 .3 9R T : 1 7 .3 5
R T : 1 5 .7 1 R T : 1 6 .6 8
N L :1 .6 8 E 7T IC M S G e n e s i s B T X _ C a l_ 07
Standard 1:10
Decane (IS)
Benzene
Toluenep/m-Xylene o-Xylene
1,3,5-TMB
Styrene1,2,4-TMB
1,2,3-TMB
1 0 00
2 0
4 0R T : 1 3 .8 8R T : 1 1 .9 9
R T : 1 1 .9 9 N L :1 .7 9 E 6T IC M S
4 0
6 0
8 0
Rel
ativ
e A
bund
ance
R T : 1 8 2 2 R T : 1 9 3 9R T : 1 7 3 5R T : 1 3 8 7 R T 1 5 7 0
G e n e s i s b tx_ c a l_ 0 1Standard 1:1000
8 0
1 0 00
2 0R R T : 1 8 .2 2 R T : 1 9 .3 9R T : 1 7 .3 5R T : 1 3 .8 7 R T : 1 5 .7 0
R T : 1 1 .9 8 N L :1 .6 9 E 6T IC M S G e n e s i s b tx_ b lw _ 0 3Blank + IS
2 0
4 0
6 0
R T : 1 3 .8 7
Blank + ISNo Carryover
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1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1T i m e (m i n )
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TriPlus RSH: substantially reducing $/sample lab costs
Automated basic sample handling and sample prep • Derivatization: introduce precise amount of reagents,
intensive agitate and automate all processintensive agitate and automate all process• Example: Biodiesel derivatization using Thermo Biodiesel kit;
Up to 24 sample vials are processed automatically - prepares 5 standard solution vials plus 2 blank vials automatically5 standard solution vials plus 2 blank vials automatically -volume compensation of the 5 standard solution vials – set 15 minutes of derivatization time per sample vial - syringe washing using dedicated solvent vialswashing using dedicated solvent vials
• Result:
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Labor costs strongly reduced while improving accuracy
TriPlus RSH : four good reasons to buy it!
• Ultimate productivity for reliable 24/7 operations• More robotic X-Y-Z precise movement controls• The largest sample capacity
• More vials can be loaded with larger solvent washing capability• Value for the investmentValue for the investment
• The highest configuration scalability• Full compatibility of all accessories with all configurations
• Substantially reducing $/sample lab costs• New unique capability to automate basic sample prep
• Dilution internal standard addition derivatization• Dilution, internal standard addition, derivatization• Even higher quality results and performance
• Full support of all Thermo injection capabilities
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TriPlus RSH: Higher quality results and performance
• Compatible with the full range of Thermo injectors:SSL/PTV/OC/PKD/PPKD
• Support all range of large volume capabilities: LVSL/PTVLVI/OCLVI
• Fast injection mode minimizes discrimination
• Support microvolumes samples, down to 5 L allowing multiple injections
• Flexible handling of sample inside syringe, for the most critical samples (air, 2nd sample, ISTD, etc.)
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Powerful versatility in sample handling and injection
TriPlus RSH: Higher quality results and performance
• Accurate injection from microvolume samples – ideal for trace POPs analysis, biological fluids, explosive residues or when an expensive external standard is used
• New bottom vial sensing capability delivers accurate and reproducible injections from a sample as low as 5 l
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Powerful versatility in sample handling and injection
NEW bottom vial sensing capability: down to with 5 L sample
3 replicates possible with 5 L sample: average RSD 1%3 replicates possible with 5 L sample: average RSD 1%
5 L sample in vial: C20-C40 Florida mix
5 replicates, 1 L eachusing the bottom sensing option
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TriPlus RSH: Higher quality results and performance
• Selectable optimum injection techniques according to sample requirements
Fast Cold Needle
requirements• Fast cold needle “liquid band”
injection (injection speed )
NEW
approach 100 ms)• Hot empty needle “thermospray”
injection (more gentle sampleHot Empty Needle
injection (more gentle sample introduction for labile and active compounds)
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Powerful versatility in sample handling and injection
New TriPlus RSH Product Capabilities
TriPlus RSH: widest sample injection capabilities
• Liquid injection:• Basic
E i h t
• Headspace:• Pre-Filling
C ti• Enrichment• Enrichment needle solvent
wash
• Continuous purge• Enrichment• Progressive
• Internal Standard double• Internal Standard post
N dl l h
Progressive• Multiple Extraction
• SPME:• Needle solvent wash• Solvent flush double• Solvent flush post
• Progressive• Multiple GC configurations:
D bl PSolvent flush post• Fast injection mode
• DoublePro• Clone mode
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Multiple configurations for enhanced GC or GC/MS productivity
2 x TRACE GC Ultra with Extended X-UnitTRACE GC Ultra with Standard X-Unit
2 x FOCUS with Standard X-UnitFOCUS + TRACE with Standard X-Unit
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TriPlus RSH Clone Mode
• The TriPlus RSH is installable on two adjacent gas chromatographs and can serve up to four injectors.
• TriPlus RSH is therefore capable of injecting into 2 DIFFERENT GC or p j gGC/MS using 2 DIFFERENT sampling methods and/or data systems, exactly as if there were 2 individual TriPlus RSH autosamplers, thus virtually “cloning” the autosampler.
• Add MS configurations for even more combinations. Here TQ and Ion Trap are shown as example
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Excellent liquid injection repeatibility10 injections overlay10 injections overlay. Splitless injections “Florida Mix”
C24H50
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Integrated Software Control for Intuitive sample workflow
• The TriPlus RSH instrument control is fully• The TriPlus RSH instrument control is fully embedded into our CDS:
• ChromQuest• Chrom-Card• Chrom-Card• Xcalibur
• Methods and sequences can be• Methods and sequences can be developed and run locally or remotely through a network card.
• Three basic setup screens guide the setting of TriPlus RSH parameters for Liquid Headspace and SPME methodsLiquid, Headspace and SPME methods.
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Consumables• Full choice of approved micro vials vials for liquid and headspace• Full choice of approved micro vials, vials for liquid and headspace,
magnetic and plastic caps, snap-on, screw- and crimp-top caps, a wide choice of liquid and gas-tight syringes, well plates, trays and much more are available.available.
• This complete selection of Thermo Scientific consumables complements our innovative range of GC and GC MS systemsour innovative range of GC and GC-MS systems.
• All of these consumables are designed and tested to work problem-free with the TriPlus RSH autosampler.
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Triplus RSH Summary
TriPlus RSH is an entirely new platform of GC and GC/MS autosamplers ThermoFisher Scientific has developed allowing:
Unattended, weekend long operationsUnattended, weekend long operations• Up to 648 2-mL vials and multiple 100 mL solvent bottles stations
Unattended, injection modes changes in a sequence: liquid Unattended, injection modes changes in a sequence: liquid –– HS HS -- SPMESPME• ATC (Automatic Tool change) Station for automatic recognition and syringe
exchanges during operation
Reliable, reproducible injections with microvolume samplesReliable, reproducible injections with microvolume samples• <2%RSD and up to 3 injections possible with 5 l sample volume in vial.
Advanced sample handling and sample preparationAdvanced sample handling and sample preparation• Dilution, derivatization, standard addition, internal standard additions, etc.
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Questions?
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