introducing the new triplus rsh...

Introducing the New TriPlus RSH Autosampler

August 21st, 2011

Introducing the new Triplus RSH Autosampler

You are Productive

Solving your Chromatography Challengesg y g p y g

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Triplus RSH at a glance

TriPlus RSH is an entirely new platform of GC and GC/MS autosamplers ThermoFisher Scientific has developed allowing:

Unattended, weekend long operationsUnattended, weekend long operations• Up to 648 2-mL vials and multiple 100 mL solvent bottles stations

Unattended, injection modes changes in a sequence: liquid Unattended, injection modes changes in a sequence: liquid –– HS HS -- SPMESPME• ATC (Automatic Tool change) Station for automatic recognition and syringe

exchanges during operation

Reliable, reproducible injections with microvolume samplesReliable, reproducible injections with microvolume samples• <2%RSD and up to 3 injections possible with 5 l sample volume in vial.

Advanced sample handling and sample preparationAdvanced sample handling and sample preparation• Dilution, derivatization, standard addition, internal standard additions, etc.

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Summary

• TriPlus RSH: four major benefits, four good reasons to buy it

T iPl RSH d t biliti• TriPlus RSH product capabilities

• TriPlus RSH Configurations• Clone Mode

• TriPlus RSH proof datap

• TriPlus RSH Software Integration

• TriPlus RSH Validated Consumables

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TriPlus RSH Autosampler: Integrated Sampling system

• TriPlus RSH (Robotic Sample Handling) is an entirely new platform of robotic autosampler

• Improved autosampler performance and li bilit f li id h d d SPMEreliability for liquids, headspace and SPME

• Accurate injection from microvolume samples

• No more users required when switching from liquid to Headspace or SPME

Runs sequences with multiple injection• Runs sequences with multiple injection modes, unattendedly changing the tool/syringe

• Sample Preparation such as Dilution, Internal standard addition, derivatization with robotic tool changing technology

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TriPlus RSH : four good reasons to buy it!

• Ultimate productivity for reliable 24/7 operations• More robotic X-Y-Z precise movement controls• The largest sample capacity for unattended sequences

• More vials can be loaded with larger solvent washing capability• Value for the investmentValue for the investment

• The highest configuration scalability• Full compatibility of all accessories with all configurations

• Substantially reducing $/sample lab costs• New unique capability to automate basic sample prep

• Dilution internal standard addition derivatization• Dilution, internal standard addition, derivatization• Even higher quality results and performance

• Full support of all Thermo injection capabilities

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TriPlus RSH : The highest precision

M b ti X Y Z i t t l• More robotic X-Y-Z precise movement controlsSolvent: Ethyl Acetate10 ul syringeLiquid taken from washing station and used to fill empty vialsTransfer per vial: 8 ul, 15 times

Typical repeatibility 0.066%

Solvent: waterSolvent: water100 ul syringeLiquid taken from washing station and used to fill empty vialsTransfer per vial: 100 ul, 1 time

Typical repeatibility 0.021%

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Indipendently from GC performance

TriPlus RSH : The highest precision

• More robotic X-Y-Z precise movement controls

Solvent: 2-propanol100 l i100 ul syringeLiquid taken from washing station and used to fill empty vialsVolume Linearity > 0.99999

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Indipendently from GC performance

TriPlus RSH : Ultimate productivity for reliable 24/7 operations

• The largest sample capacity, ensuring true, weekend-long operations

• Multiple tray holders: • Up to 648 2-mL vials

U t 840 0 7 0 5 L i i l• Up to 840 0.7- or 0.5-mL micro vials

• The largest solvent washing capabilityThe largest solvent washing capability• Four different 10-mL solvent vials to

maximize cleaning efficiency• Multiple 100-mL solvent bottles

stations for high sample throughput, with waste collected externally

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• Ultimate productivity for reliable 24/7 operations• More robotic X-Y-Z precise movement controls• The largest sample capacity






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TriPlus RSH Autosampler: Value for the investment

B i Li id fi ti B i h d fi ti B i d fi tiBasic Liquid configuration Basic headspace configuration Basic duo configuration

With 162 2-mL vials capacity

With 60 10- or 20- mL vials capacity

With 60 10- or 20-mL and 162 2-mL vials capacitycapacity

Standard liquid syringe tool (10 L)

Standard washing

capacity

HS gas tight syringe tool (2.5 mL)

Agitation and Incubation

2 mL vials capacity

HS gas tight syringe tool (2.5 mL) and standard liquid syringe tool (10 L)Standard washing

station (5 10-mL vials)Agitation and Incubation oven, up to 6 10- or 20-mL vials up to 200°C

Agitation and Incubation oven, up to 6 10- or 20-mL vials up to 200°C

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Standard washing station (5 10-mL vials)

TriPlus RSH Autosampler: Value for the investmentBasic Liquid confBasic Liquid conf fully compatible with

Agitator and Heated f HS SPME

Peltier heating or cooling of samplesoven for HS, SPME or

sample prepcooling of samples

SPME fiber conditioning for SPME applications

More sample trays for higher capacity

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for SPME applications higher capacityVortexer for Intensive sample mix

Barcode reader for sample tracking








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TriPlus RSH: substantially reducing $/sample lab costs

N A t ti T l Ch i (ATC) St tiNew Automatic Tool Changing (ATC) Station• Unique ability to automatically handle up to six different syringes

Unattended syringe exchange during a sequence

ATC Station

• Unattended syringe exchange during a sequence • Zero carryover and no compromise in sample preparation

ATC Station

TR (Tool Releasing) Station for manual

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syringe exchange


Automated basic sample handling and sample prep • Sequential dilution: dilute a commercial standard to

meet the analytical requirementmeet the analytical requirement

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Labor costs strongly reduced while improving accuracy

TriPlus RSH: Substantially Reducing $/sample Lab Cost

Automated basic sample handling and sample prep • Calibration dilution: automatically prepare calibration

points adding internal standardspoints adding internal standards

ISTD

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Calibration Dilution

0.08 – 4 µg/mL 0.08 – 80 µg/mL

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R T : 1 1 .0 6 - 2 1 .0 4R T : 1 8 2 2 N L :1 3 5 TMB 1 2 3-TMB

Calibration Dilution

4 0

6 0

8 0

1 0 0R T : 1 8 .2 2

R T : 1 9 .3 9R T : 1 7 .3 5

R T : 1 5 .7 1 R T : 1 6 .6 8

N L :1 .6 8 E 7T IC M S G e n e s i s B T X _ C a l_ 07

Standard 1:10

Decane (IS)

Benzene

Toluenep/m-Xylene o-Xylene

1,3,5-TMB

Styrene1,2,4-TMB

1,2,3-TMB

1 0 00

2 0

4 0R T : 1 3 .8 8R T : 1 1 .9 9

R T : 1 1 .9 9 N L :1 .7 9 E 6T IC M S

4 0

6 0

8 0

Rel

ativ

e A

bund

ance

R T : 1 8 2 2 R T : 1 9 3 9R T : 1 7 3 5R T : 1 3 8 7 R T 1 5 7 0

G e n e s i s b tx_ c a l_ 0 1Standard 1:1000

8 0

1 0 00

2 0R R T : 1 8 .2 2 R T : 1 9 .3 9R T : 1 7 .3 5R T : 1 3 .8 7 R T : 1 5 .7 0

R T : 1 1 .9 8 N L :1 .6 9 E 6T IC M S G e n e s i s b tx_ b lw _ 0 3Blank + IS

2 0

4 0

6 0

R T : 1 3 .8 7

Blank + ISNo Carryover

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1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1T i m e (m i n )

0


Automated basic sample handling and sample prep • Derivatization: introduce precise amount of reagents,

intensive agitate and automate all processintensive agitate and automate all process• Example: Biodiesel derivatization using Thermo Biodiesel kit;

Up to 24 sample vials are processed automatically - prepares 5 standard solution vials plus 2 blank vials automatically5 standard solution vials plus 2 blank vials automatically -volume compensation of the 5 standard solution vials – set 15 minutes of derivatization time per sample vial - syringe washing using dedicated solvent vialswashing using dedicated solvent vials

• Result:

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TriPlus RSH: Higher quality results and performance

• Compatible with the full range of Thermo injectors:SSL/PTV/OC/PKD/PPKD

• Support all range of large volume capabilities: LVSL/PTVLVI/OCLVI

• Fast injection mode minimizes discrimination

• Support microvolumes samples, down to 5 L allowing multiple injections

• Flexible handling of sample inside syringe, for the most critical samples (air, 2nd sample, ISTD, etc.)

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Powerful versatility in sample handling and injection


• Accurate injection from microvolume samples – ideal for trace POPs analysis, biological fluids, explosive residues or when an expensive external standard is used

• New bottom vial sensing capability delivers accurate and reproducible injections from a sample as low as 5 l

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NEW bottom vial sensing capability: down to with 5 L sample

3 replicates possible with 5 L sample: average RSD 1%3 replicates possible with 5 L sample: average RSD 1%

5 L sample in vial: C20-C40 Florida mix

5 replicates, 1 L eachusing the bottom sensing option

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• Selectable optimum injection techniques according to sample requirements

Fast Cold Needle

requirements• Fast cold needle “liquid band”

injection (injection speed )

NEW

approach 100 ms)• Hot empty needle “thermospray”

injection (more gentle sampleHot Empty Needle

injection (more gentle sample introduction for labile and active compounds)

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New TriPlus RSH Product Capabilities

TriPlus RSH: widest sample injection capabilities

• Liquid injection:• Basic

E i h t

• Headspace:• Pre-Filling

C ti• Enrichment• Enrichment needle solvent

wash

• Continuous purge• Enrichment• Progressive

• Internal Standard double• Internal Standard post

N dl l h

Progressive• Multiple Extraction

• SPME:• Needle solvent wash• Solvent flush double• Solvent flush post

• Progressive• Multiple GC configurations:

D bl PSolvent flush post• Fast injection mode

• DoublePro• Clone mode

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Multiple configurations for enhanced GC or GC/MS productivity

2 x TRACE GC Ultra with Extended X-UnitTRACE GC Ultra with Standard X-Unit

2 x FOCUS with Standard X-UnitFOCUS + TRACE with Standard X-Unit

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TriPlus RSH Clone Mode

• The TriPlus RSH is installable on two adjacent gas chromatographs and can serve up to four injectors.

• TriPlus RSH is therefore capable of injecting into 2 DIFFERENT GC or p j gGC/MS using 2 DIFFERENT sampling methods and/or data systems, exactly as if there were 2 individual TriPlus RSH autosamplers, thus virtually “cloning” the autosampler.

• Add MS configurations for even more combinations. Here TQ and Ion Trap are shown as example

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Excellent liquid injection repeatibility10 injections overlay10 injections overlay. Splitless injections “Florida Mix”

C24H50

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Integrated Software Control for Intuitive sample workflow

• The TriPlus RSH instrument control is fully• The TriPlus RSH instrument control is fully embedded into our CDS:

• ChromQuest• Chrom-Card• Chrom-Card• Xcalibur

• Methods and sequences can be• Methods and sequences can be developed and run locally or remotely through a network card.

• Three basic setup screens guide the setting of TriPlus RSH parameters for Liquid Headspace and SPME methodsLiquid, Headspace and SPME methods.

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Consumables• Full choice of approved micro vials vials for liquid and headspace• Full choice of approved micro vials, vials for liquid and headspace,

magnetic and plastic caps, snap-on, screw- and crimp-top caps, a wide choice of liquid and gas-tight syringes, well plates, trays and much more are available.available.

• This complete selection of Thermo Scientific consumables complements our innovative range of GC and GC MS systemsour innovative range of GC and GC-MS systems.

• All of these consumables are designed and tested to work problem-free with the TriPlus RSH autosampler.

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Triplus RSH Summary

TriPlus RSH is an entirely new platform of GC and GC/MS autosamplers ThermoFisher Scientific has developed allowing:

Unattended, weekend long operationsUnattended, weekend long operations• Up to 648 2-mL vials and multiple 100 mL solvent bottles stations

Unattended, injection modes changes in a sequence: liquid Unattended, injection modes changes in a sequence: liquid –– HS HS -- SPMESPME• ATC (Automatic Tool change) Station for automatic recognition and syringe

exchanges during operation

Reliable, reproducible injections with microvolume samplesReliable, reproducible injections with microvolume samples• <2%RSD and up to 3 injections possible with 5 l sample volume in vial.

Advanced sample handling and sample preparationAdvanced sample handling and sample preparation• Dilution, derivatization, standard addition, internal standard additions, etc.

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Questions?

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