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Page 1: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Good Laboratory Safety Practices

Page 2: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Buddy System

Be Safe! Don’t be alone when working with hazardous materials or in dangerous work conditions. Your life can depend on it!!

Page 3: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Buddy System

When performing potentially hazardous/dangerous operations always: – Make sure someone is in the

laboratory with you – Notify someone that you are in the

lab and what time you will check with them when you complete your work.

– Check-in with that person if you exceed your time estimate or if you leave.

Page 4: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

A Clean Lab is a Happy Lab

Page 5: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

A Cluttered Lab is a Dangerous Lab!

Keep your lab clean!

Page 6: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Housekeeping The Results of Poor housekeeping: Accidents

– Cluttered counter tops are a good source of spills.

– Poorly maintained equipment or apparatus can break spilling its contents, damage other equipment or flying debris can cause injury.

– Containers and equipment on the floor are a trip and spill hazard.

Page 7: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Housekeeping The Results of Poor

housekeeping: Costs you money

– Overstocking or reordering of chemicals due to uncontrolled or unmanaged inventory.

– Replacing spilled chemicals or damaged equipment.

Page 8: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Housekeeping The Results of Poor housekeeping: Contaminates your research

– Poorly washed containers or equipment can lead to

cross contamination. – Poorly labeled storage containers can lead to the use of a wrong reagent.

Page 9: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Housekeeping Poor housekeeping: Causes accidents: Costs you money Contaminates your

research Sets up bad habits for

students entering a more regulated industrial setting.

Gets more attention from inspectors.

Page 10: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Don’t Block Access to Electrical Panels, Safety Showers and Eye Wash Stations

Page 11: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Don’t block doors or Fire Extinguishers

Page 12: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Unattended Bunsen burner causes bench fire.

Never leave lit Bunsen burners unattended or use flammable solvents near them.

Page 13: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Old Equipment Can Be Dangerous

Rusty water-bath with exposed wires

Maintain your Equipment!

Page 14: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Improper Chemical Storage Ether was stored in

this domestic style refrigerator. Either the thermostat or the interior light was the ignition source that caused the explosion.

Flammables have to be stored in a Flammables or Explosion proof fridge.

Page 15: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Read labels before pouring containers

Or this … …turns into this.

Page 16: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Lack of Attention to Details

Heater/stir plate being used to stir only accidentally was also turned on as hot plate also!

Page 17: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Incomplete reaction placed in refrigerator – BANG!

See the glass imbedded In the door?

Reaction container was placed into the refrigerator before the reaction had been completely quenched. The bottle over pressurized and exploded.

Page 18: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Do Not Store Consumables with Chemicals!

Page 19: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Proper Equipment Usage There needs to be a written Standard Operating

Procedure for all equipment. Everyone should be properly trained before

using any equipment. Improperly used or maintained equipment can

be dangerous to your health or can result in expensive damages.

Fires are common result with inappropriate equipment usage.

Page 20: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Centrifuges- An Example of Improper Usage and Maintenance

Page 21: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Hazards

Mechanical failure of rotating parts (often violent).

Contact with rotating parts. Sample leaks causing aerosols, stress corrosion,

contamination. Sample imbalance causing machine movement /

walking (or stress failure of component parts). Fire or explosion. Health (contact with contaminated components /

vapors). Ergonomics/lifting

Page 22: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Rotor Failure

Page 23: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Rotor Failure

Page 24: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Rotor Failure

Page 25: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Contained Rotor Failure

Page 26: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Dancing Centrifuge

This can be caused by an improperly balanced rotor.

Page 27: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

Remember Good Practices

Never work alone. Commit to good housekeeping Do not block access to doors, electrical or

emergency equipment Store chemicals and wastes properly Maintain equipment

Page 28: Good Laboratory Safety Practices - bfa.sdsu.edu · laboratory with you – Notify someone that you are in the lab and what time you will check with them when you complete your work

The End

12/2/14


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