1-3

http://cafe.daum.net/cancerchaga

2

1980 . . 1980 15 . 1980 1983 .

()

. 1994 - . ,

3

, , .

(Kirst Kahlos) . 1987 1997 . . .

.

4

(Chaga) 16, 17 (Minaeva 1970).

,

1950 1960 . 1950 1960 . . (, 1966).

[ 1]

- (Kirsti Kahlos) 1987

5

. :

1.

2.

.

, , , . , .

, . (1963) ,

6

. (1963) , 18 . . .

1950 ,

. (Befungin) , , 1955 . ( 1973). , .

7

(1962) , . , , . 180, MOP 755 .

granylosytic , mycolocytes . , RC 83 . Kier(1961) , . 180 755 .

8

. . (23 ), 45 (40-80 ) .

. , 13C NMR .

9

10

[ ]

180

755(3) . (1), (2) (3) - .

13C-NRM ,

, .

[ 2]

- , R. M. v. Schantz

11

[ ]

(Pers. ex Fr.) , , 3-hydroxylanosta-8, 24-dien-21- (1, 2) .

[ 3]

- R.

12

[ ] 3, 21-

dihydroxy-lanosta-8, 24- diene 3, 22, 25-trihydroxy-lanosta-8, 23-diene n- . . IR, MS, 1H NMR 13C NMR .

[ 4] ,

- - , SF-00170 ,

, , -

13

[ ] 3, 22-dihydroxy-lanosta-8,

24-dien-7-one . IR, MS, 1H NMR 13C NMR . 7- NMR . .

[ 5] 3,22-DIHYDROXY-LANOST A-8,24-DIEN-7-ONE, 7-

- - , SF-00170 , ,

,

14

[ ] , 3, 22-dihydroxy-lanosta-8, 24-

diene (Pers. ex Fr.) n- , . Walker 256 carcinosarcoma ( ) MCF-7 adenocarcinoma mammae ( ) . 50g/ml 100% .

[ 6]

- - -

15

, 3, 22-dihydroxy-lanosta-8, 24-diene (Pers. ex Fr.) . .

. (ascites fluid) (4). HTC 373 (5).

Walker 256 rat carcinosarcoma MCF-7 human breast cancer( ) . MCF-7 , , .

16

, . IR, MS 1H NMR . Walker 256 , , . 50g/ml Walker 256 , 30% .

5 , ,

. Walker 256 MCF-7 2, 4, 5 ; MCF-7 , 50g/ml .

17

18

[ ] (Pers.

ex Fr.) ( 1959). .

[ 7] n-

- - - - , 00170 , ,

, - , Farmos LTD,

P.O. Box 425, 00100 Turku,

19

( 1966). , ( 1961) , ( 1983) (Ludwiczak Wrzeciono 1960, 1985) .

(1962)

, .

Alnusoregona Nutt.

Walker 256(5WAI6) , Sarracenia flava L. nasopharynx(KB)(Steth 1973, 1974) . Walker 256 carcinosarcoma MCF-7

20

humal adenocarcinoma mammary 100% ( 1986).

n-

MCF-7 Walker 256 , P 388 .

rat Walker 256 carcinosarcoma (gift of

Prof. Osswald, Deutsches Krebsfor-schungszentrum, , FRG) MCF-7 (, , ).

[ ] MCF-7 Walker 256

MCF-7 Walker 256

4,5 . ,

21

(G-J) . 4,5 2-4 II .

3-hydroxy-lanosta-8,24-dien-21-al Walker

256 . MCF-7 . 4 30%.

3,21-dihydroxy-lanosta-8,24-diene

. , Walker 256 carcino -sarcoma 80%, MCF-7 human mammary adenocarcinoma 60%.

Wawlker 256

, MCF-7 . 50g/ml 90% .

22

3, 22, 25-trihydroxy-lanosta-8, 23-diene Walker 256 . MCF-7 , 50g/ml 4 .

(G-J) MCF-7

. 3, 22-dihydroxy-lanosta-8, 24-dien-7-one(Fr. G), 3, 22-dihydroxy-lan osta-7, 9(11), 24-triene(Fr. I) (Fr. H J) 50g/ml 4 .

23

24

,

methione . . MCF-7 (adenocarcinoma) mammae : C-21 (trametenolic ) > C-21 > C-21

[ 8] cysteine cysteine C-21 (Inonotus obli-quus)

- - V.H. Tikka

25

> methyltramenenolate > > . Methylation (methyltrametenolate) c-21 5 95% , .

26

[ 9]

- - , P.O Box 56, (Viikinkaari 5),

00014, Biocenter 2, , .

- A. LESNAU, W. LANGE - Nationales Referenzzentrum fur Influenza,

Robert Koch Institut, D-13353, Nordufer 20, , .

- U. LINDERQUIST - Institut fur Pharmazeutische Biologie,

Friedrich Ludwig Jahn Str. 15a, D-17487, Greifswald, Germany.

27

. . .

. . .( )

A B, A(A/H1N1, A/H3N2, A/Equine2, B//16/18 ) .

28

(100%) . betulin lupeol, mycosterols .

(Betula)

, . .

, .

. .

29

triterpenes , lunapes triterpenes . Inotodiol(3, 22--8, 24-diene) (3--8, 24-diene) C-21 triterpenes .

triterpenes .

Inotodiol Walker 256 carcinosarcoma MCF-7 , .

A B .

EtOH(

2%) . 40g/ml .

30

20g/ml . . A . B . [ ]

.

intodiol, , 3--8, 24-dien-21-al 3 , 21--8, 24- diene( 1) .

, , taraxerone, ,

, fungisterol, -7, 22-dien-3-ol, -, 3, 22--7, 9(11), 24-triene .

31

( 1) (A) (B) triterpenes GC (%)*

A B 0.8 0.4

0.8 0.8 Taraxerone 3.5 -7,22-dien-3--ol 1.1 1.3 Fungisterol 0.2 Lupenone 5.8 12.6 0.5 - 0.9 1.9 Lupeol 4.2 1.9 3, 22--7,9(11),24-triene-Inotodiol 3.8 1.7

3, 21--8,24-diene 39.9 23.5 +3--8,24-dien-21-al 14.9 36.3 2.1 11.7 Triterpene () 3.8 2.6

* triterpenes A: B: -7, (9)11, 24-triene 8-

lanostanes . triterpenes

32

, .

2.1% betulin

betulin . Lano-stanes , . Taraxerone(3.5%) . Betulin triterpene . lupines, lupeol lupanone . Taraxeranes, lupanes -sitosterol Alnus .

betulin

, pentacyclic . 30% betulin 8.5% lupeol , (B.b ) lanostanes .

33

.

40g/ml A B A .( 2)

( 2) (A/H1N1, A/H3H2) (A/Equine2)

n- (%) ( ) (g)

( ) A/H1N1 Q/H3H2 A/Equine2

A B B.a B.b

0.42 0.34 0.25 0.26

0.1081 1.0430 0.0747 0.1294

0.21 0.60 0.13 0.23

0 0 100 0

0 0 100 0

35 0 100 0

*A: (A. glutinosa); B: (B pubescens); B.a:;B.b:

34

20g/ml A/H1N1/86 1.7 log 10 ID50, B/Yamagata/88 1.8 log 10 ID50 . 1 log 10 ID50 .

Betulin, mycosterols,

, A/H1N1 (60-100%). . .

35