Download - A 3-week Biotech Unit for Any Biology Class Ellyn Daugherty SMBCP, San Mateo CA Educator/Author
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A 3-week Biotech Unitfor Any Biology Class
Ellyn DaughertySMBCP, San Mateo CA
Educator/Authorwww.BiotechEd.comwww.SMBiotech.com
www.emcp.com/biotechwww.sargentwelch.com/biotech
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Biotech in Biology3 weeks (15 hours)
> What is Biotechnology? Computer Search Collage
> Micropipeting Activity
> DNA Spooling Activity
> DNA Fingerprinting Using RFLPs Activity
> Recombinant DNA Technology
> Bacterial Transformation (GMO) Activity
> Bioethics of Engineering Organisms
> Careers in Biotechnology
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Materials, Supplies, Funds Needed for Biotech in Biology Unit
Class set of:
•4 micropipets/tips each (P-1000, P-200, P-20)
•4 gel boxes, 2 power supplies
•DNA Spooling Activity Kit
•DNA Fingerprinting Activity Kit
•Bacteria Transformation Activity Kit
•Other supplies and materials (96-well plates, food coloring, 37°C incubation oven, water bath
= about $300 in kits/ class= about $3000 for school set of micropipets and gel apparatus
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What Is Biotechnology?
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Micropipeting Activity
Show me:
Pipet 135 µL
or 235 µL
into a tube
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What Does DNA Look Like?
(Edvotek #S-10)
= one 50 min class period (30 min activity, 20 min discussion
$39 / 10 lab groups
DNA Spooling Activity
Do Onions Have DNA (or other fruits
or veggies) ?
(Edvotek #S-75)
= one 50 min class period (30 min activity, 20 min discussion
$49 / 5 lab groups… or use my Lab 4b Materials from SW List
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DNA Fingerprinting Using RFLPs Activity
Ward’s DNA Detectives Lab Activity #109)
= four 50 min class periods
$115 / 8 lab groups
Edvotek’s
DNA Fingerprinting by Restriction Fragmentation Patterns #109)
= four 50 min class periods
$79 / 6 lab groups
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Recombinant DNA
Technologyuse
http://www.dnalc.org/resources/animations/
Use the Restriction Digestion animation to discuss:
• Restriction enzymes
• DNA ligase
• Recombinant DNA (rDNA)
Use the Transformation animation to discuss:
• Insertion of rDNA vector
• Selection and Expression of newly insert genes
• Applications of rDNA technology
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Bacterial Transformation (GMO) Activity
Ward’s Improved Bacterial Transformation Using GFP #36 V 6359
= four 50 min class periods
$85/ 8 lab groups
Edvotek’s Transformation with Green Fluorescent Protein
#223 and #969
= four 50 min class periods
$110/ 10 lab groups
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Biotechnology Career Research e-Project
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Bioethics of Engineered Organisms
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Materials Lists
www.sargentwelch.com/biotech
Find your Sales Rep for materials/equipment/
support
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Biotechnology: Science for the New Millennium
Ellyn [email protected]
•Text with Encore CD•Lab Manual•Instructor’s Guide •Course Planner (Lesson
Plans)•Instructor Resource Center
on web•Student Notebook, Test
Generator, etc. available
www.BiotechEd.com for tour through the entire curriculum, ancillaries, websites, and professional development opportunities