To cite this paper: Saeed M, Ahmad F, Asif Arain M, Abd El-Hack M-E, Emam M and Ahmed Bhutto Z (2017). Use of Mannan- Oligosaccharides (MOS) As a Feed Additive in

Poultry Nutrition. J. World Poult. Res., 7 (3): 94-103. 94

JWPR Journal of World’s

Poultry Research

2017, Scienceline Publication

J. World Poult. Res. 7(3): 94-103, Sept 25, 2017

Review, PII: S2322455X1700012-7

License: CC BY 4.0

Use of Mannan- Oligosaccharides (MOS) As a Feed Additive in

Poultry Nutrition

Muhammad Saeed1*, Fawwad Ahmad

2, Muhammad Asif Arain

1,3*, Mohamed E. Abd El-Hack

4, Mohamed Emam

5, Zohaib

Ahmed Bhutto3 and Arman Moshaveri

6

1Department of Animal Nutrition, College of Animal Sciences and Technology, Northwest A&F University, Yangling 712100, China 2Department of Poultry Science, Institute of Animal and Dairy Sciences, University of Agriculture, Faisalabad, Pakistan

3Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences, 3800, Uthal, Balochistan, Pakistan

4Department of Poultry, Faculty of Agriculture, Zagazig University, 44511, Zagazig, Egypt 5Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt

6Department of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran *Corresponding author’s Email: muhammad.saeed@nwafu.edu.cn

Received: 09 Jul 2017

Accepted: 14 Aug 2017

ABSTRACT

The European Union banned using all prophylactic antibiotics as growth promoters in poultry nutrition. As a result,

the poultry nutritionist is now forced to look for growth promoting antibiotic alternatives, or at least considerably

demote the amount of antibiotics used to sustain efficient broiler meat production and to be able to produce safe

poultry egg and meat products. The Mannan-oligosaccharides (MOS), is a type of probiotics originated from the

yeast cell wall (Saccharomyces cerevisiae) has gained more prominent attention, mainly due to its ability to bind the

threadlike fimbriae on pathogenic bacteria preventing them from attaching to the gut wall, thereby averting their

stabilization and the resulting colonization and multiplication, up to the disease level, so it had been showed to be a

most capable solution for antibiotic-free diets, as well as furnishing effective support for digestion and immunity in

poultry. Several investigations confirmed that using MOS as a feed supplement in poultry diets allowed birds to

achieve a similar trend as when they were fed a diet enriched with antibiotic growth promoters. In addition, MOS has

also shown to have a positive affection on bodyweight gain, feed conversion ratio, egg weight, egg production,

fertility, and hatchability thus ameliorating well-being, energy levels and performance of avian species. Furthermore,

it is also thought that it plays a role as an antioxidant, helping with mineral retention, improving bone mineralization

and subsequently the overall improvement the performance of poultry birds. This review article has aimed to

illuminate its sources, mode of action and beneficial applications of MOS in poultry diet for improving, production,

immunity, safeguarding health among consumers and it ought to be used as a natural growth promoter on a

commercial level in order to replace synthetic antibiotics in the poultry industry.

Key words: Antioxidant, Feed additive, Gastrointestinal health, Mannan-oligosaccharides (MOS), Performance,

Poultry

INTRODUCTION

In the past decades, a variety of feed accretive had

been employed in poultry diet. These feed accretive led to

an improved rendition and effective utilization of feed in

poultry birds (Chand et al., 2016a; Shah et al., 2016; Xing

et al., 2017; Saeed et al., 2017a, b). Routinely being

utilized in accretive of feed as: emulsifiers, antimicrobials,

antioxidants, biological products, herbs, pH control agents

binders and enzymes as well (Vahdatpour and Babazadeh,

2016; Siyal et al., 2017; Tareen et al., 2017; Saeed et al.,

2017c, d, e).

Growth promoting is not the only use of feed

additives but they have used also for stabilizing the

95

beneficial gut microflora by forestalling beneficial

microorganisms (Hashemi and Dawoodi, 2011; Abudabos

et al., 2017). In the last decades, antibiotics that are used

as growth promoters in animal feed have been under

severe attention, since they pose a potential threat to

consumers by generating resistance in the host against the

bacteria (Sultan et al., 2015). Conclusively, the European

Union had banned the supplementation of growth

promoting antibiotics in the animal diet since 2006 (Khan

et al., 2016). Now, it is most important for the poultry

researcher to find alternatives to antibiotic growth

promoters to boost the health andproduction

performanceof poultry birds (Janardhana et al., 2009;

Babazadeh et al., 2011; Vahdatpour et al., 2011). Feed

additives of plant origin have gained a great interest in the

poultry industry as they are safer, with wide dose range

and so rare adverse effects (Alzawqari et al., 2016;

Abudabos et al., 2016). Recently, many experiments had

shown a number of significant effects on growth

parameters, immune response and gut health status in birds

fed diets contain phytogens (Tanweer et al., 2014; Saeed et

al., 2015; El-Hack et al., 2016, Saeed et al., 2017f, g , h).

These studies have shown that the small intestine with the

main role in the absorption of nutrients; it then proves that,

both the proper structure and the proper function of the

intestine is efficient in improving poultry performance and

health (Sultan et al., 2014). It has been suggested that

intestinal digestion and absorption of the nutrients is

higher if the surface area of the villi is increased (Chand et

al., 2016b). The beneficial microflora of young birds gut’s

are counted to be somewhat irregular and can easily be

disturbed by several external factors. The subclinical

infection is one of these external factors which posed by

the pathogenic challenge. So, the ability to preserve an

optimal or normal level of beneficial microflora in the gut

becomes one of the main factors in the determination of

the ultimate health status and consequently the genetic

growth expression of poultry. At commercial basis,

available mannan-oligosaccharide has exhibited to

enhance the bird growth parameters including feed intake

and feed utilization (Hooge, 2004a; Rosen, 2007a, b;

Nikpiran et al., 2013). The beneficial impacts of MOS on

the development gut microflora were also revealed by

Kocher et al. (2005) and Yang et al. (2008). The addition

of MOS constantly elevates the caecal beneficial

populations like Bifidobacterium and Lactobacillus spp.

(Sadeghi et al., 2013). Decreasing the pathogenic bacteria

and the increasing the beneficial bacteria could be

belonged to the receptor sites competition and producing

volatile fatty acids by bacteriocins along with IgA

antibodies by the host immune system (Kim et al., 2009).

Owing to these changes in the beneficial microflora, the

goblet cells number and intestinal villi length increase as

well, which ultimately promotes functions and health of

the host GIT (Bonos et al., 2010). The diet supplemented

with MOS has been reported to have a positive effect

regarding body weight, feed efficiency, egg yield, fertility,

egg mass and egg hatchability in various poultry species

(Guclu, 2011; El-Samee et al., 2012). In another study by

Iqbal et al. (2017) who had fed birds with MOS that had

significant effects on body and egg mass, egg weight,

and egg number and it has shown that feeding MOS as a

substitute for antibiotics, as growth enhancer, can

positively impact productive traits as well as health

aspects in breeders of quail. This can also improve the

manifest utilization of energy in feed and improvethe birds

feed efficiency that could partially belong to the

modulatory impacts of mannan-oligosaccharide on the

GIT microflorain broilers (Yang et al., 2008). The current

review article discusses the potential aspects of using

MOS; including its sources, mode of action and beneficial

applications of MOS and its practical uses in the nutrition

and production of poultry industry for improving,

production, immunity and safeguarding health, among

consumers and to prioritize this natural growth promoter

as opposed to synthetic antibiotics to cope the medicinal

cost in poultry.

Chemical traits and source of mannan-

oligosaccharides

Mannan-oligosaccharides originated from the

mannose blocks that exist in the yeast cell wall as it is

mostly non-digestible carbohydrates (Saccharomyces

cerevisiaeis). The cell wall consists of up to 25–30% of

cell dry weight. The Saccharomyces cerevisiae is known

yeast in the brewery and bakery industries. The MOS

product which is a derivative of the yeast is used in animal

nutrition. Saccharomyces cerevisiae cell wall involves

both α-glucans and mannan-proteins. The essential

building block for yeast cell wall are polymers of mannan

with α (1-2) and α (1-6) bonds and to a less extent α (1-3)

bounded side chains (Kogan and Kocher, 2007). The host

enzymes or the intestinal bacteria enzymes cannot break

these bonds apart and as a result carbohydrates (MOS)

have no direct nutritive value, but it has benefits in

keeping the gut health. It can be theorized from the several

scientific research work that although mannan as a

derivate from yeast (Saccharomyces cerevisiae) is

attributed to production and processing technologies, it

might have different chemical formation and biological

efficiency as reported by Spring (1999).

96

Mode of action

The beneficial microbiota development and the

sustainment of eubiosis act an important role in the

mechanisms of defense in the body and health of gut as

well. There is elevating evidence confirming that the

composition of microflora in the gastro intestinal tract in

an adult healthy host remains statistically stable as

theorized by Williams et al. (2001). Results of current

studies suggesting that the supplementation of MOS to

poultry diets can minimize the count of hind gut

pathogenic bacteria during the high exposure to the

pathogen (White et al., 2002; Castillo et al., 2008). The

MOS supplementation was indeed accompanied with

increasing beneficial flora, especially lactobacilli (Rekiel

et al., 2007). Another experiment has also confirmed the

beneficial impact of MOS, however, it has been also found

to decline animal gut concentration of ammonia

(Juskiewicz et al., 2003). Literature documented data

indicated that dietary MOS fed diets can greatly lower the

number of pathogens. In some studies on poultry, proves

found that if the dietis supplemented with MOS a

considerable positive effect on gut histological structure in

broilers chicken (Iji et al., 2001a). Similarly, it is reported

that dietary supplementation of mannan products had the

effect of increases the ratio of villous height/crypt depth in

young broilers (Iji et al., 2001a; Yan et al., 2008) and in

turkeys as well (Ferket, 2002) (Figure 1). Nochta et al.

(2010) found that the addition of mannan as feed

supplement remarkably enhanced the nutrients apparent

digestibility.

Figure 1. How do Mannan-Oligosaccharides (MOS) affect intestinal structure (MOS could prevent the colonization and

attachment of pathogenic bacteria and thus reduce the adverse effects of microflora and metabolites)

Beneficial effects of mannan-oligosccharides in

poultry

Broiler Farming

Effect on growth performance and blood

biochemistry. Mannan-oligosaccharide that is one of the

best alternatives to antibiotic growth advancers in the

poultry industry diets and which are originated from yeast

outer cell wall that known as Saccharomyces cerevisiae

(Esecel et al 2012). The use of MOS in broiler diets had

shown to positively impacts on performance criteria

(Rosen, 2007a; Fritts and Waldroup, 2003). The range of

dietary inclusion of the MOS averaged from 0.5 to 5 g /kg

diet. The dose-response of MOS in different research work

had showed the best dosage of MOS for optimal growth is

around 2 g /kg diet as reported by Tucker et al. (2003). Iji

et al. (2001b) studied the influences of different doses of

MOS (0, 1, 3 and 5 g /kg diet) on the structure and

function of the intestine of poultry birds within the starter

97

period (21-day). Results proved that poultry birds gave a

high response by increasing the addition of MOS from 1 to

21 d compared to the 21-42 d period (Tucker et al., 2003).

Nikpiran et al. (2014) reported that adding the MOS to the

diets of poultry improved the growth performance values

by enhancing the feed intake and stimulating the growth

hormone and insulin release.

In a study had reported a significant decrease in the

total cholesterol concentration in broiler chickens which

had been supplemented with MOS @ 0.05% when

compared to a control diet (Juskiewicz et al., 2003). Also,

another experiment had shown that MOS could promote

caecal Lactobacillus spp. and Bifidobacterium spp. growth

and also elevated the height of villus and the number of

goblet cells in poultry jejunum and ileum (Mohsen et al.,

2014).

Effect on immune response. It is found that MOS

had proved to be much more effective on antibody

production against Avian Influenza Virus (AIV) in broiler

chickens than Humate (HU). The immune function could

be augmented with dietary Humate and MOS

supplementation (Tohid et al., 2010). The innate immune

system recognizes key molecular formations of the

invading bacteria involving peptidoglycans,

lipopolysaccharides, and possibly the structures of

mannose in the cell walls of yeasts. Oligosaccharides

which have mannose have been reported to impact on

immune system through activating mannose-binding

protein secretion from the liver. The aforementioned

protein, as a result can enchain to bacteria and trigger the

complement cascade of the immune system of the host as

described by Newman (1994). MOS was indicated of

having a beneficial effect on both immunoglobulin status

and humoral immunity in general. Savage (1996)

described an increase in IgG of the plasma and bile IgA in

poultry grown up on diets supplemented with 0.11%

MOS. The diet fed with MOS may constitute a novel and

most effective plausible alternative that could reduce the

spread of disease by decreasing the virus shedding and the

contamination of the environment from AIV (H9N2)

infection in poultry birds (Akhtar et al., 2016). Both

Saccharomyces cerevisiae and its derived product is

known as MOS that supplementation in poultry feed has a

clear effect on the attenuation of Escherichia coli (E. coli)

which induces intestinal cells disruption by reducing the

intestinal inflammation and barrier dysfunction in broilers

chicken. In addition to that, yeast (Saccharomyces

cerevisiae) addition could also improved the intestinal

microbiota and feed efficiency of in avian species (Wang

et al., 2016) and MOS could improve the absorption of

trace minerals (Sohail et al., 2011).

Layer farming

The feed supplementation with MOS has shown to

entail positive effects by improving (P < 0.01) the liver

antioxidant status and mitigating the significant increase in

the cecal pathogenic bacterial load after molt in layer birds

which shows the benefits of which can be improved

with MOS supplementation (Bozkurt et al., 2016). The

prebiotic (mannan-oligosaccharide) supplementation can

positively alter the intestinal microenvironment (Hutsko et

al., 2016). In another study by Jahanian and Ashnagar

(2015) found that MOS supplementation to laying hens

feed under bacterial infection could improve their

productive performance probably through modification in

the gut’s bacterial populations and improving nutrient

digestibility. As described by Bozkurt et al. (2012) who

had shown that egg production had efficiently improved

by MOS also showed that a stimulating humeral immune

response in laying hens in different climate conditions.

Turkey farming

After the broiler production industry, the turkey

industry considered as the second source of poultry meat

across the globe. In turkeys, 76 numbers of comparisons

showed the same responses to MOS as in broilers (Hooge,

2004b; Rosen, 2007b). Hooge (2004b) claimed that MOS

addition to turkey rations revealed an average increase in

body weight by2% and reductionmortality by about25%.

So, organic enteric conditioners, such as dietary

MOS, are of great importance for the turkey farming

industry. Recently, antibiotic resistance had been raised in

the Escherichia coli exist in the field which had been

isolated from commercial turkey farms in North Carolina.

In addition to that, a resistance to the Enrofloxacin had

been showed (Bernick et al., 1999). There is no specific

proof that that growth promoting doses of antibiotics

control disease (Gustafson and Bowen, 1997), the debate

over the Gram-negative bacteria that had been showing

some resistance, as shown by Salmonella and E. coli,

which caused the strongest objection to the use of

antibiotic as growth promoters (Scioli et al., 1983). MOS

improves the performance of turkey poults, especially

during the E. coli challenge like antibiotics which were

traditionally used (Ferket et al., 2002). An improvement in

growth performance was also observed in turkeys fed diets

enriched with MOS (Savage and Zakrzewska, 1996) also

authors found a statistical increase in body weight gain in

large white male poults which fed a diet supplemented

with 0.11% MOS. Cetin et al. (2005) reported that MOS

98

enhanced immunoglobulin levels and caused more

positive effects on growth performance, production and

the turkeys' ability to resist diseases. As a result of the

previous finding, it can be concluded that MOS is an

interesting alternative to antibiotic growth promoters to

improve performance in turkey (Parks et al., 2001) and

also it has a clear effect on improving body weight gain

and lowering mortality in poults (Hooge 2004a).

An alternative to antibiotic

In contrast, regarding the action mode of the

chemical growth promoters (antibiotics) fermentable

carbohydrates sources, oligosaccharides especially MOS,

act as one of the best alternatives to the Gram-negative

pathogens attachment sites, so they prevent the attachment

to the enterocytes and subsequently prevents the enteric

infection. The adherence step of the pathogenic microbe

to the intestinal cell wall is known to be the prerequisite

step to the infection (Gibbons and Houte, 1975). This can

be more clarified as in Vibrio cholera which is incapable

of starting their disease signs without the attachment step

to the enterocyte, even with large numbers of bacteria

present (Freter, 1969). The adhesion step causes the

bacterial entrapment and colonizing. The entrapment of

nutrients for growth, the concentration of the digestive

enzymes and the toxins onto intestinal cell wall, and the

possible prevention of antibody attachment to the

pathogenic cell (Costerton et al., 1978). The cell wall of

the yeast organism is mostly carbohydrates and proteins in

the form of mannose, glucose, and N-acetylglucosamine

that are branched and chained together (Ballou, 1970).

Mannan-oligosaccharides that are derived from mannans

on yeast cell surfaces are acting effective binder to the

bacterial binding sites (Ofek et al., 1977). Pathogens that

are mannose-specific Type-1 fimbriae are confused and

adsorbed to the MOS, leaving the enterocytes without

colonization. In the study of Newman (1994), that had

shown that the presence of dietary mannan-

oligosaccharides in the intestine had successfully

discarded some pathogenic bacteria that had the possibility

of attachment to the lumen of the intestine. Mannose was

shown by (Oyofo et al., 1989a) to inhibit the in vitro

attachment of Salmonella Typhimurium to intestinal cells

of the day old broilers chicken. A study by (Oyofo et al.,

1989b) had shown that dietary mannose had a successful

effect on inhibiting Salmonella Typhimurium in intestinal

colonization in broilers. (Spring et al., 2000) had shown an

effort in screening different bacterial strains to examine

their ability to agglutinate mannan-oligosaccharides in

yeast cell preparations (Saccharomyces cerevisiae, NCYC

1026). Which showed that the inclusion of MOS in the

diet can improve the poultry birds' performance, especially

during challenging with E. coli, as well as being used as

growth promoter antibiotics in poultry industry? A

comparison of some attributes with dietary mannan-

oligosaccharides and antibiotics is shown in table 1,

(Ferket et al., 2002).

Table 1. Comparison of some attributes with dietary

mannan-oligosaccharides and antibiotics.

Antibiotics Mannan-oligosaccharides

It reduces the non-specific

immunological protection in the

mucosa as a result of reducing

both beneficial and non-

beneficial bacteria (i.e.

lactobacilli)

It can increases non-specific

mucosal immunological

protection by increasing

relatively the goblet cell

numbers and consequently

the mucus secretion and it

increases the colonization of

beneficial bacteria in the gut.

It improves AME and reduces

the energy needed for

maintenance which

consequently improves the net

energy availability

It improves net energy

available for production by

improving dietary AME

It improves growth

performance parameters under

various environmental

conditions

Improves growth

performance parameters

mainly when challenged

with enteric pathogens

By suppressing enteric

microflora it suppresses the

competition for the nutrients.

It improves the brush border

health so it enhances the

absorption process.

prolonged or Improper usage

can produce antibiotic resistant

pathogens

It will not produce bacterial

resistance

Reduces immunological stress

via lowering enteric microbial

load

It's important role to

stimulate gut-associated

system immunity by acting

as a non-pathogenic

microbial antigen

Decreases adverse effects of

microflora metabolites by

decreasing the microflora

Decreases the adverse effects

of microflora metabolites by

changing microflora profile

It inhibits both the viability and

proliferation of some pathogens

and beneficial enteric

microflora

It acts as a barrier against the

attachment and consequent

colonization of some enteric

bacteria, but it is not

bactericidal.

99

Figure 2. A flow diagram illustrating a large surface area is vital for optimal digestive function and nutrient absorption in

poultry birds.

CONCLUSION

After reviewing the compiled literature it can be fully

clarified that MOSs can be considered as a potential

alternative to antibiotic growth promoters, and even at

trace amounts @ 0.1%-0.4% practically usage as

commercial feed additive in poultry nutrition would be

quite effective in improving the health status and

production performance of poultry. Among consumer

concerns about danger increasing of antibiotic-resistant

pathogens has urged the poultry nutritionist to consider

“biologically safer” alternatives After studying the

published literature it is clear now that MOS is considered

one of the best alternatives to antibiotic growth promoters.

These mannan-oligosaccharides are non-digestible

carbohydrates that may have greater benefits than

antibiotics if it is used in a synergic way with other non-

pharmaceutical enteric conditioners, such as fructo-

oligosaccharides, probiotics, bioactive peptides, and some

herbs and it would, in this manner, be a helpful additive to

reduce feed cost in the poultry industry.

Acknowledgements

All the authors of the manuscript thank and

acknowledge their respective Universities and Institutes.

Competing interests

Authors declared that they have no conflict of

interest.

Author`s contributions

All the authors significantly contributed to compile

and revise this manuscript. MS, MAA, reviewed the

literature and initiated the review compilation. MEAEH,

ZAB and ME, critically revise the manuscript. FA and

MEAEH check the English language accuracy. Finally all

authors read and approve the manuscript for publication.

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