Gene Expression Profiling in Patients with Chronic Obstructive Pulmonary Disease

and Lung Cancer

I-Ming Wang1ψ, Sergey Stepaniants1ψ, Yves Boie2, James R Mortimer2, Brian Kennedy2,

Mark Elliott3, Shizu Hayashi3, Leanna Loy3, Silvija Coulter1, Sandra Cervino1, Jennifer

Harris2, Michele Thornton1, Richard Raubertas4, Chris Roberts1, Jim C Hogg3 , Michael

Crackower2, Gary O’Neill2 and Peter D Paré3

1Rosetta Inpharmatics, 2Merck Frosst Canada, 3UBC James Hogg iCAPTURE Centre,

Providence Health Care, and 4Merck Frosst Rahway

ψ I-Ming Wang and Sergey Stepaniants contributed equally to this study

Correspondence to Dr Peter D Paré (Rm 166, McDonald Research Wing, St. Paul’s

Hospital, 1081 Burrard St., Vancouver, BC, Canada V6Z 1Y6 Tel:604-806-8346,

Fax:604-806-8351, email: ppare@mrl.ubc.ca)

Supported by the Canadian Institute for Health Research and Merck Frosst Canada

Running Title: Gene expression profiling in COPD

Descriptor Number: 51

Word Count: 5,290

This article has an online data supplement, which is accessible from this issue’s table of content online at www.atsjournals.org

AJRCCM Articles in Press. Published on November 1, 2007 as doi:10.1164/rccm.200703-390OC

Copyright (C) 2007 by the American Thoracic Society.

GENE EXPRESSION PROFILING IN COPD

Abstract: Rationale: Chronic Obstructive Lung Disease (COPD) is a common and disabling lung

disease for which there are few therapeutic options. We reasoned that gene expression

profiling of COPD lungs could reveal previously unidentified disease pathways.

Methods and Main Results: 48 human lung samples were obtained from tissue resected

from 5 non-smokers, 21 GOLD 0, 9 GOLD 1, 10 GOLD 2 and 3 GOLD 3 patients.

mRNA from the specimens was profiled using Agilent's Functional ID v2.0 array

containing 23,720 sequences. The gene expression pattern was influenced by the

percentage of the sample made up of parenchyma. Gene expression was related to Forced

Expiratory Flow between 25 and 75% of forced expiratory volume (FEF 25-75 % P)

revealing a signature gene set of 203 transcripts. Genes involved in extracellular matrix

synthesis/degradation and apoptosis were among the up-regulated genes whereas genes

which participate in anti-inflammatory responses were down-regulated.

Immunohistochemistry confirmed expression of urokinase (PLAU), urokinanse receptor

(PLAUR) and thrombospondin (THBS1) by alveolar macrophages and airway epithelial

cells. Genes in this pathway have been shown to be involved in the activation of

transforming growth factor beta-1 (TGF [beta] 1) and matrix metalloproteinases (MMP)

and are subject to inhibition by SERPINE 2. Interestingly, both TGF beta 1 and

SERPINE 2 have been identified as candidate genes in COPD genetic linkage and

association studies.

Conclusion: The results provide evidence that genes involved in tissue remodeling and

repair are differentially regulated in the lungs of obstructed smokers and suggest that they

are potential therapeutic targets. (249 words)

Key words: Pulmonary emphysema, phenotype, transcriptional analysis, cigarette

smoking

1

Clinical Trial Registry Information: ID# GSE8500 registered at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=ldolzqeugcwegfs&acc=GSE8500

GENE EXPRESSION PROFILING IN COPD

Introduction: Chronic obstructive lung disease (COPD) is characterized by airflow limitation that is not

fully reversible: the airflow limitation is usually progressive and associated with an

abnormal inflammatory response of the lungs to noxious particles and gases (1, 2). This

abnormal chronic inflammatory immune response affects both airways and parenchyma

and is characterized by thickening, fibrosis and narrowing of the small conducting

airways and emphysematous destruction of the lungs’ elastic properties. (3)

Although it is generally agreed that inflammation plays a role in both the

parenchymal and airway components of the disorder, traditional anti-inflammatory

therapy has proved unsuccessful in modifying the progression of the disease. (4) New

treatments for COPD under development include antagonists for LTB4, 5-LO, IL-8,

TNFα and iNOS. In addition, a panel of new anti-inflammatory reagents such as

inhibitors of PDE4, NF-κB, p38, PI3K and neutrophil elastase are being actively pursued

as potential therapeutics (5). Although several of these have been tested in clinical trials,

none of them has emerged as an effective drug.

The lack of success of traditional therapies suggests the need for a new paradigm

and novel approaches to treatment. One method for generating new ideas about the

pathogenesis of complex genetic disease is to perform genome wide transcriptional

analysis on diseased tissue for comparison with normal tissue. This unbiased approach

allows hypothesis generation unrestrained by existing schemas for pathogenesis but

generates large volumes of data which are challenging to analyze and interpret. In

addition careful selection of subjects and samples is required to obtain reproducible

results. A number of investigators have used the technologic advances in genome-wide

expression platforms to compare the lungs of patients who have COPD with those of non-

diseased subjects. (6-8)

In the present study we took advantage of a well characterized group of patients in

whom detailed lung function, lung morphometry and frozen lung tissue were available to

examine whole lung tissue gene expression patterns using the Agilent microarray

technology. By using quantitative morphometric analysis to determine the tissue-type

content of the actual samples we found a subset of genes which was related to the

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GENE EXPRESSION PROFILING IN COPD

percentage of the sample made up of parenchyma versus airways and blood vessels. We

also derived a list of 203 genes whose level of expression was related to lung function,

(FEF 25-75 % P) after correction for age, sex and smoking. Among other genes, this

approach identified urokinase (PLAU), urokinase receptor (PLAUR) and

thrombospondin 1 (THBS1) as potential targets in COPD. Immunostaining of the same

tissue samples showed expression of PLAU, PLAUR and THBS1 in alveolar

macrophages, alveolar epithelial cells and the epithelium of the small airways. The small

airway epithelium of smokers with airflow obstruction showed more extensive staining

for PLAUR than the epithelium of the non obstructed smokers.

Comparison with previously published studies of genome-wide transcriptional

analysis (6-8) showed little overlap for differentially expressed genes. This lack of

reproducibility suggests that factors such as disease severity, sample acquisition, tissue

type and expression platform influence the results. A standardization of such factors will

be necessary for meaningful comparisons across studies.

A portion of the results of this study have been presented previously in abstract

form at the American Thoracic Society Annual meeting. (9)

Methods.

Subject selection: The patients for gene profiling were selected from a larger group of

patients who required pneumonectomy or lobectomy for small peripheral lung nodules

and who are part of an ongoing investigation of lung structure and function (10, 11).

Criteria for inclusion were that the total volume of the lung lesion, as calculated from

radiographs and/or CT scans, was less than 120 mL and that the lesions did not obstruct

subsegmental or larger airways. Patients who had radiographic or pathological evidence

of obstructive pneumonitis were excluded. A total of ~731 subjects fulfilled these criteria

and gave informed consent to have their clinical and lung function data, along with their

resected tissue, examined in research studies using methods approved by the Providence

Health Care Clinical Ethics Review Board. Of these subjects 184 had their resected lung

tissue frozen and archived in a manner suitable for gene expression studies. From these

184 subjects we selected 74 subjects to represent a range of smoking and lung function.

We randomly selected 6 of the 12 non-smokers in the biobank and randomly sampled ~1/3 of

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GENE EXPRESSION PROFILING IN COPD

those in the lower GOLD categories (GOLD 0-1), ~1/2 of those in GOLD 2, and all 3 of the

subjects in GOLD 3. Of these 48 were found to have mRNA of suitable quality for gene

expression profiling (Figure 1).

The 26 subjects whose RNA did not pass quality control criteria did not differ

from those included in the study with respect to age, male/female ratio, FEV1% predicted

or pack years smoked (63.4±2 years versus 62.8±2; 11/15 versus 17/31 Male/Female;

86.8±4 versus 86.5±3% predicted and 34.4±6.3 versus 39.1 ±3.2 pack years, respectively

– p> 0.2 for all comparisons). In addition there was no difference in the time from tissue

collection to RNA extraction for those samples which yielded high or lesser quality RNA.

Prior to surgery subdivisions of lung volume, spirometry and single breath

diffusing capacity were measured as previously described and according to ATS

standards. (10) A modified ATS questionnaire was applied to gather demographic and

clinical information. A detailed smoking exposure was determined and expressed as

pack years. Based on smoking history and lung function subjects were classified as

lifetime non-smokers or into the GOLD categories of COPD severity (3). We expressed

forced expiratory flow between 25 and 75% of forced expiratory volume as a percent of

the predicted value (FEF 25-75 % P) (12) to use as a continuous variable for comparison

with gene expression data. Table 1 shows the number of patients in each GOLD category

and their mean FEF 25-75 % P.

Tissue processing: Immediately following resection, the lung or lobe was

obtained from the operating room and after the clinical specimens of the lesion, lymph

nodes and the resection margin were obtained the lobes and lungs were inflated using a

50% mixture of CryomatrixR and saline and frozen in liquid nitrogen fumes. The frozen

lungs and lobes were then cut into 7-15 two cm thick slices using a band saw and

multiple randomly stratified blocks were acquired (1-3/slice) using a power driven hole

saw fitted with a 1.5cm diameter bit (11). The frozen “cores” were stored at -80o C for

later cryosectioning and RNA extraction. (see online supplement for details)

Frozen sections were obtained from the surface of the ½ core immediately

adjacent to the portion used for RNA extraction. The 10 micron sections were stained

with hematoxylin and eosin and a digital image of the entire sample was captured. The

4

GENE EXPRESSION PROFILING IN COPD

fraction of the tissue area occupied by air and tissue (% parenchyma) was determined

and the tissue content was further divided into alveolar tissue, membranous airway,

cartilaginous airway and blood vessel tissue using a standard point counting method (13).

Microarray study design and methodology: RNA from 8 of the 21 GOLD 0 subjects

(non-obstructed smokers) was pooled to form the reference RNA. (the eight were chosen

because enough RNA was available from their lung sample). The reverse transcribed

cDNA from this reference was used for competitive hybridization against all of the other

samples including all of the GOLD 0 samples which made up the reference pool.

Microarray profiling was done for 23,720 sequences as previously described (14) and as

outlined in the online supplement.

Immunohistochemistry: Frozen human lung specimens were cut at 10 µm thickness

onto slides and stored at -70°C until used. For PLAUR and THBS1 staining the APAAP

method was used while for PLAU the ABC method (DAKO Handbook) was employed.

Details are included in the online supplement.

Image Analysis: For PLAU and PLAUR, all images were captured using a Nikon

Eclipse E600 microscope fitted with a SPOT camera. The image analysis was performed

using Image-Pro Plus 4.0 (Media Cybernetics, Silver Spring, MD). Alveolar

macrophages positive for PLAU and PLAUR were expressed as a percent of total

macrophages and the length of the membranous bronchiolar epithelium which stained

positively for these proteins was expressed as a percent of the total epithelial length.

Only selected sections were stained for thrombospondin. Details are included in the

online supplement.

Statistical Analysis:

In order to identify genes correlated to lung function we used a continuous variable, the

post bronchodilator FEF 25-75 % P. This measure of lung function provided the largest

range of values, and as, is shown in table 1, related well to GOLD stage. Among

demographic and clinical variables which were potentially predictive of FEF 25-75 % P

5

GENE EXPRESSION PROFILING IN COPD

(age, gender, smoking duration) we found that only exposure to cigarette smoke,

expressed as pack years, was significantly negatively related (r= -0.5 p < 0.0005) as

shown in Figure 2. Thus in selecting genes related to FEF 25-75 % P we corrected for any

relationship with pack years by fitting the data to a multivariate linear model:

σ)ε(0,PACKYEAR2

β P% 75-25 FEF1β

0βY +++=

Where Y is gene expression for each gene, β0 is the intercept, β1 and β2 are slope

coefficients for FEF 25-75 % P and pack years respectively and ε is random noise with zero

mean and standard deviation σ. We were primarily interested in the genes which showed

a significant relationship to FEF 25-75 % P adjusted for pack years. The significance of this

relationship is given by the p-value for the slope of FEF 25-75 % P as well as overall p-

value of the model. Since microarrays do not provide absolute readouts of gene

abundance, the model intercept is strongly related to the type of hybridization pool used

and is therefore of no specific interest. Since there was a significant influence of the

tissue content (% parenchyma) on gene expression profile we also used a model in which

% lung parenchyma is included as well as FEF 25-75%P and Pack Years.

The extent of PLAU and PLAUR staining was compared to FEF 25-75 % P and pack years

using linear regression analysis and, in addition, the mean % positive macrophages and %

positive airway epithelial length in subjects who had a FEF 25-75 % P <60% predicted

were compared to those who had a FEF 25-75 % P >60% predicted using Student’s t-test.

TaqMan validation: A number of the genes whose level of expression proved to be

related to the level of lung function (COPD signature genes) were tested using TaqMan

real-time PCR. Details of the TaqMan protocol are available in the on-line supplement.

Results

Study subjects: Forty two of the subjects had lung resection for bronchial carcinoma,

five subjects had carcinoid tumors and one subject had resection of an amyloid lesion.

Table 1 shows demographic and clinical data for the 48 subjects on whom mRNA

profiling was done. The non-smokers were younger than the smokers but there was no

difference in age between GOLD categories. Most of the subjects were in the GOLD 0, 1

6

GENE EXPRESSION PROFILING IN COPD

and 2 categories. This distribution is consistent with our archived samples and reflects

the fact that the majority of heavy smokers do not develop severe airflow limitation and

that severely obstructed subjects are not candidates for lung resection.

Tissue Samples: All of the tissue samples showed good expansion allowing accurate

quantification of tissue compartments. The tissue type content of the samples varied.

Some were pure parenchyma while others showed substantial percentages of airways and

blood vessels. The mean content of “air” was 69±13 % (range 18-87%) and the

remainder was made up of tissue which was subdivided into parenchymal tissue,

membranous bronchi, cartilaginous bronchi and blood vessels larger than capillaries. The

parenchyma made up 75±19% (range 7-95%), membranous bronchioles 2.5±2.9% (range

0-11%), cartilaginous bronchi 1.7±4% (range 0-19%) and blood vessels 19±15% (range

0-68%) of the total tissue area. Of the 48 samples 30, 12 and 47 contained some

membranous bronchiolar, cartilaginous bronchial and/or blood vessel tissue, respectively.

Microarray

All 48 samples passed Rosetta’s quality control (See on-line supplement for details).

Microarray hybridizations comparing all 48 lung samples to the control pool of 8 GOLD

0 non-obstructive smokers showed. 3,222 sequences with a significant difference in

expression at a fold change difference ≥ 1.5, P-value ≤ 0.01 in more than 5 patients using

the Rosetta Error Model (15). 2-D cluster analysis, however, did not result in a co-

clustering of samples derived from patients of the same GOLD stage. Approximately half

(22 out of 48) of the samples contained a signature which was highly enriched with

tracheal genes, obtained from monkey and human body gene expression atlases. (data not

shown)

The effect of the percentage of the tissue sample made up of lung parenchyma on the

gene expression was assessed for all transcripts by correlating individual transcripts to the

% parenchyma. 5159 genes correlated with % parenchyma with a correlation coefficient r

≥ + 0.3 or – 0.3. (see online supplement) The significance of this correlation, p ≤ 0.05,

was evaluated using a Monte Carlo permutation (14). Eight-seven percent of the genes in

the “tracheal signature” subset were represented in this category. These data indicate that

the gene expression profile of individual tissue samples was influenced by their tissue

7

GENE EXPRESSION PROFILING IN COPD

content; samples with a low parenchymal content, and therefore a higher content of

airways and blood vessels, contained a unique subset of “tracheally” expressed

transcripts.

Lung Function-related gene expression patterns

203 genes whose level of expression was related to lung function were selected on the

following basis; 1) a level of expression average across all experiments with logIntensity

> -1.5, abs(logRatio) > 0.2; 2) Rosetta error model p-value < 0.05 in 4 or more

experiments; and 3) a p < 0.02 for the independent relationship of expression level with

FEF 25-75 % P as well as a p < 0.02 in the model which incorporated FEF 25-75 % P and

pack years. Figure 3 shows a heat map of these “COPD signature” transcripts and their

relationship to FEF 25-75 % P and GOLD stage. A list of the 203 COPD signature genes in

rank order of their relationship with FEF 25-75 % P is available in the online supplement. A

sample relationship between FEF 25-75 % P and the level of expression of urokinase

receptor (PLAUR), an example of one of the COPD signature genes, is shown in figure 4.

To examine if tissue content confounded the relationship between gene expression and

lung function we also analyzed a model which included % parenchyma as a variable. The

inclusion of % parenchyma did not substantially impact the slopes and p-values for the

relationships between the level of expression of the 203 transcripts and FEF 25-75 %P.

(See on-line supplement)

Taqman validation: To validate the results of the microarray eight genes selected from

among the 203 COPD signature genes were subjected to TaqMan real-time quantitative

PCR in 3-7 subjects using the same RNA sample that was used in profiling. (CXCL1,

IL8, THBS1, PLAU, PLAUR, CCL2, BMPR2 and MMP9). Although the level of

expression relative to the pooled reference sample was systematically higher using

TaqMan, the relationship was highly concordant. The equation relating the two methods

was: Microarry value = 0.69 TaqMan value + 0.48 ( R2 = 0.63). (For details see on-line

supplement Table E1 and Figure E1.)

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GENE EXPRESSION PROFILING IN COPD

Immunohistochemisty: To select promising candidate genes for further analysis we

developed a scoring scheme which included the following factors; 1) the fold difference in

mRNA levels between obstructed and unobstructed smokers, 2) the ANOVA p values, 3) the

abundance of the mRNA, 4) location of the gene in a locus linked to a COPD phenotype, 5)

altered expression in more than one candidate in a pathway, 6) the biologic plausibility that the

candidate could contribute to the pathogenesis of a COPD, 7) reported COPD-like phenotype

in a mouse model if over-expressed or knocked out, 8) altered expression in one or more

inflammatory disorder other than COPD and 9) attractiveness as a therapeutic target. Based on

this schema we selected PLAU, PLAUR and THBS1 for validation at the protein level using

immunohistochemistry.

Immunohistochemical staining for PLAU and PLAUR was performed on sections from

each of the 48 lung samples which were profiled. Staining for thrombospondin was

performed on a few selected specimens. Figure 5 shows representative sections

illustrating that both PLAU and its receptor were strongly expressed in alveolar

macrophages and in bronchiolar epithelial cells. Staining was also detected, to a lesser

extent, in alveolar surface epithelial cells (not shown) and circulating inflammatory cells

within the capillaries. (Figure 6). The majority of the macrophages expressed both

PLAU (68± 32%) and its receptor (67± 25%). Similarly most of the length of the

bronchioloar epithelium stained positively for both PLAU (83 ± 25%) and PLAUR (76 ±

29%). The extent of staining for PLAU and PLAUR was correlated in both macrophages

(Pearson correlation coefficient = 0.59 for % positive) and epithelial cells (Pearson

correlation coefficient =0.40 for % positive). There was no significant correlation

between FEF 25-75 % P or pack years and the number, or percent, of alveolar macrophages

positive for PLAU or PLAUR. Similarly there were no significant correlations between

the percent of the length of the bronchiolar epithelium that was stained for PLAU and

PLAUR and the measurements of lung function or smoking. However when subjects

were divided into those whose FEF 25-75 % P was less than, and more than 60%, the

obstructed smokers showed greater bronchiolar epithelial staining for PLAUR (Table 2).

The 60% predicted cut off is arbitrary but the rationale was that a FEF25-75 %P < 60 is

clearly abnormal. The 95% confidence lower limit in the normal population is ~70%

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GENE EXPRESSION PROFILING IN COPD

predicted. In addition this divided the group into two with a reasonable number in each

group; 21 versus 27.

Selected sections were stained for thrombospondin and the pattern of staining was similar

to that for PLAU and PLAUR. Alveolar macrophages and membranous bronchiolar

epithelium were positive with no staining of other cell types (data not shown).

Network analysis of COPD signature genes: The 203 genes which were related to lung

function were uploaded into the Ingenuity Pathways Analysis tool for gene network

search. Figure 7 shows a portion of the resulting network. This analysis showed a number

of pathways containing two or more differentially expressed genes which are biologically

plausible candidates for the pathogenesis of COPD. For example, PLAU, PLAUR and

THBS1, 3 genes involved in the activation of TGFβ1 and MMPs (16,17), were

significantly increased in individuals who had lower values for FEF 25-75 % P. PLAUR

resides on chromosome 19 in a locus linked to early onset human COPD in a recent study

(18). EGFR, a receptor tyrosine kinase linked to COPD (19) was down-regulated as was

the anti-inflammatory gene, SCGB1A1/uteroglobin. Many genes within this network,

including EDG5, HIPK2, FOXO3A, GAS, BDNF, FAS, PLSCR1 and BAX, are

associated with apoptosis, a mechanism implicated in the pathogenesis of COPD (20).

Two cytoskeleton assembly-related genes, YWHAH and YWHAZ, and a calcium

modulating gene (CAMLG) involved in T cell activation, were up-regulated. Finally,

PTGS2/COX-2, a well-characterized inflammatory mediator gene, was also increased.

(1,398 words)

Discussion

The study of differential gene expression in normal and diseased tissue has been

proposed as a method to identify novel pathogenetic pathways in complex disease as well

as to confirm suspected disease processes. The rationale is that gene pathways involved

in the processes which lead to tissue injury and/or repair will be activated or suppressed

in the tissue of subjects who have disease. This approach can be pursued in a hypothesis

driven format by selecting known genes or pathways for analysis or by using a genome-

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GENE EXPRESSION PROFILING IN COPD

wide unbiased approach which is designed to identify genes and pathways involved in a

disease which were not previously implicated in its pathogenesis. This approach has

been made possible by advances in genome-wide transcriptional analysis platforms and

has been successful in identifying potential new asthma targets by profiling in the

ovalbumin sensitized animal model (21).

In this study we used FEF 25-75% P for comparison with gene expression data in patients

whose lung tissue was resected for small peripheral lung lesions. FEF 25-75%P was

employed as the measure of airflow obstruction because it provided a wider range of

values than FEV1% predicted. Although it is recognized that there is a larger between-

and within-subject variation in FEF 25-75 % P it is also recognized to be a sensitive

indicator of airflow obstruction. The subjects that we studied all had lung function that

was considered adequate to undergo lung resection and thus represent a selected

population who has relatively preserved lung function. The use of FEV1%Predicted as

the measure of lung function in our patient group has a smaller dynamic range.

Nevertheless we also used FEV1% predicted as the measure of lung function for

correlation with gene expression and found substantial overlap of the genes which

correlated with both measures of airflow obstruction. (see online supplement)

We used a model to select genes whose expression correlated with lung function

independently of pack years smoked. Although there is often a poor correlation between

the level of exposure to cigarette smoke and lung dysfunction, the level expression of

many genes is increased by exposure to cigarette smoke (22-25). Correcting for the effect

of smoking on gene expression reveals genes whose expression is related to the effects of

smoking rather than the degree of smoking.

Since most of the subjects in this study had lung cancer as the reason for their lung

resection the results cannot be easily extrapolated to the broader COPD population. Spira

et al (26) have recently shown that there is a cancer-specific gene expression profile in

cytologically normal large-airway epithelial cells from patients who have pulmonary

carcinoma. Although it is likely that the genes whose expression correlates with lung

11

GENE EXPRESSION PROFILING IN COPD

function in smokers with cancer will also correlate with lung function in smokers without

cancer, this remains to be tested.

Gene profiling of whole lung samples has the power to detect differential gene expression

from any of the lung cell types but the very fact that more than 12 cell types contribute to

the expression signature makes interpretation of the results challenging. In addition to

resident cell types the lung contains an abundance of migratory inflammatory cells and

the expression pattern of whole lung tissue represents an amalgam of expression by all of

these cell types. In addition the process of development of COPD involves many years of

chronic injury and repair and the expression pattern from a gene profiling exercise is

restricted to one cross sectional look at complex processes which could have varying time

courses. Additional complexity is related to non-uniformity of lung structure and the

known spatial heterogeneity of the pathologic processes which underlie COPD.

COPD is a functional diagnosis and at least two processes, parenchymal destruction and

inflammatory airway narrowing and scarring combine to cause the abnormal function.

Tissue samples subjected to gene profiling may contain varying amounts of alveolar

tissue, blood vessels and airways and have varying pathologic involvement of these

constituents. In addition lung gene expression patterns could be altered over the short

term by environmental factors unrelated to the processes causing tissue injury. These

include exposure to cigarette smoke, microbial agents, changes in nutrition and the use of

pharmaceutical agents.

In this study we attempted to minimize these confounding factors by studying a

relatively large number of well characterized subjects and by performing a precise

quantitative characterization of the tissue type content of the profiled samples. This was

especially important since the samples were relatively small compared to those sampled

in previous studies (6-8). An advantage of profiling RNA extracted from a whole lung or

lobe is that regional differences in tissue content and disease process are averaged,

however a disadvantage is that relevant signals may be diluted or masked. The

importance of quantitative morphometry was supported by the concordance between

tissue content and the expression levels for genes from tracheal tissue derived from body

atlases of gene expression. Most body atlases studies lump “lung” as one tissue type, but

12

GENE EXPRESSION PROFILING IN COPD

as our analysis shows, a “lung” sample can contain a variable amount of specific tissue

elements and this correlates with the relative expression of tissue components (eg tracheal

signature in samples with a higher content of airways and blood vessels). Although the

tissue content did not substantially alter the relationship between lung function and gene

expression in this study, the failure to account for tissue content could lead to spurious

results if one component is over-represented in some samples and/or one compartment is

predominantly involved in a disease process.

In an ideal experimental design samples would come from patients who are

matched for age, gender, smoking and medical history. In addition, samples should be

collected from a similar region of lung with comparable proportions of structural

components. To select for disease-related genes, clear end-point differences such as

FEV1 or extent of emphysema should be observed between the control and the diseased

samples. When working with an archived tissue bank, few of these criteria can be met

(Table 1). However with the availability of body atlases and histological information, we

were able to ascertain that a major signature from these samples was related to the

relative amount of non-parenchymal tissue that they contained.

The COPD signature gene set that we identified is relatively small, but included

several genes in pathways known or suspected of being involved in the pathogenesis of

COPD. The portion of the network analysis shown in Figure 7 reveals a potential role for

the urokinase plasminogen activator (PLAU/uPA) system. This protease and its receptor

have been suggested as candidate genes for COPD. (27, 28) PLAUR anchors PLAU at

the cell surface to participate in extracellular matrix degradation, cell migration, cell

adhesion and cell proliferation (29). This ligand-receptor pair plays a critical role in the

development and persistence of inflammatory and immune responses (16). Active PLAU

proteolytically converts inactive plasminogen to active plasmin, which then activates

latent forms of MMPs and TGFβ1 (30). PLAU also interacts directly with THBS1,

another up-regulated gene related to lung dysfunction, and modulates its activity (31).

THBS1 has previously been shown to be a major activator of TGF β1 in vivo (17) and is

also an inducer of MMP9 (32). It is conceivable that the net result of this up-regulated

gene set, which includes PLAU, PLAUR, and THBS1, is a significant increase in the

activities of both TGF β1 and MMP9.

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GENE EXPRESSION PROFILING IN COPD

A review of the current literature supports the concept that dysregulated

expression of both TGF β1 and MMP9 could be involved in the pathogenesis of COPD

(33 ,34). In addition PLAUR is located on chromosome 19 in a region which has been

linked to early onset COPD (18) and PLAU is subject to inhibition by SERPINE2,

another candidate gene which is located within a COPD-linked locus on chromosme 2q.

Finally DeMeo et al (35) have shown association of polymorphisms in SERPINE2 and

COPD phenotypes. Taken together, these data and our results suggest that the PLAU-

PLAUR system could represent a potential new area for therapeutic intervention in

COPD.

There are few reports concerning the expression and function of PLAU and

PLAUR in the human lung. In infant respiratory distress syndrome which is characterized

by intra-alveolar fibrin deposition, the ratio of plasminogen activator-inhibitor-1(PAI-1)

to PLAU in tracheal aspirates was higher than in controls and immunohistochemical

analysis demonstrated increased expression of both PAI-1 and PLAU, predominantly in

alveolar epithelium (36). These results suggest a role for PLAU in intra-alveolar

fibinolysis, and are supported by studies in mice in which lung-specific over-expression

of PLAU led to a reduction in the accumulation of collagen in the lung and reduced

mortality after bleomycin-induced lung injury (37). The same transgenic mice were

studied as a model of emphysema by inducing PLAU expression over 30 weeks. These

animals showed increases in lung compliance, lung volume and alveolar size, which are

typical features of emphysema, however, because these same changes were documented

in mice which expressed only the transgene that controls lung specific expression (the

Clara cell secretory protein promoter controlling the reverse tetracycline transactivator

gene), these emphysematous changes could not be solely attributed to the overexpression

of PLAU (38). Murine studies also suggest a protective role for PLAU/PLAUR in

bacterial infection of the lung; (39-41); bacterial infections frequently complicate COPD

and may contribute to progressive disease (42).

TaqMan gene expression analysis was concordant with the microarray data for

PLAU, PLAUR and a number of additional genes. Immunostaining of sections of the

same samples that were profiled showed widespread expression of these proteins

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GENE EXPRESSION PROFILING IN COPD

predominantly in alveolar macrophages and airway epithelium, although expression was

also detected in the alveolar epithelium and circulating inflammatory cells.

Quantification of PLAU and PLAUR expression by morphometry showed a moderate

increase in expression of PLAUR in the airway epithelial cells of individuals who had

more severe airflow limitation. The lack of tight correlation of mRNA and protein

expression assessed by immunohistochemistry is not surprising. Immunohistochemisty is

an inherently non-quantitative technique. It is excellent to detect the presence or absence

of expression of a protein but when there is relatively abundant expression it fails as a

quantitative measure.

Exploration of Figure 7 also shows that a number of genes involved in apoptosis were

differentially expressed. Several pathways in the network emanate from EGFR. How

decreased expression of this growth factor receptor could result in increased expression

of its direct downstream effectors is counter-intuitive; however, the possibility of a

compensatory reaction by these targets is a possibility. The increased expression of the

phospholipid scramblase 1, PLSCR1, which mediates migration of

phosphatidylserine/ethanolamine to the outer leaflet of the plasma membrane (43) and of

FAS support the concept that apoptosis may contribute to the emphysematous destruction

of the lung parenchyma in COPD (reviewed in 44). Similarly two additional upregulated

genes (YWHAH & YWHAZ) are involved in apoptosis through interaction with a

ubiqutin-protein ligase, parkin (45) and regulation of cytoskeletal architecture (46). On

the other hand the results show decreased expression of FOXO3A as airway obstruction

progresses. The FOXO family of Forkhead transcription factors is regulated by the

phosphoinositide 3 kinase/protein kinase B (PI3K/Akt) pathway and plays a key role in

controlling cell cycle, apoptosis, oxidative stress and immunomodulation (47). Down

regulation of FOXO3A would be expected to be pro-inflammatory, but anti-apoptotic.

which is consistent with its downregulation by FAS and the pro-inflammatory phenotype

of COPD (48).

Other genes of interest include the Coxsackie virus and adenovirus receptor (CXADR)

which was found to be up-regulated in the lungs of obstructed subjects. In addition to

serving as the receptor that mediates viral attachment to the cell surface and viral uptake,

it also plays a role in cell adhesion, paracellular solute movement, cell proliferation, and

15

GENE EXPRESSION PROFILING IN COPD

cell signaling (49). Adenovirus, especially its E1A protein, has been implicated in the

pathogenesis of COPD and high levels of E1A DNA were located in the lungs of COPD

patients and its level of expression increased with disease severity (50). Consistent with a

previous report (51), COX-2 was up-regulated in COPD lungs and could potentially

contribute to the inflammatory status of the disease.

Three COPD-related microarray studies have been recently published (6-8). The genes

which we report to be correlated with lung function showed little overlap with the genes

whose level of expression has been correlated with lung function in these reports. A

comparison of the study designs and results is included in the on-line supplement. These

differences could relate to differences in patient selection, tissue sampling and gene

expression platform.

The unique features of this study are that 1) we profiled tissue from a relatively large

group of individuals who were well characterized pre-operatively, and 2) we related the

gene expression pattern to the tissue content of the samples that were profiled. Our

results not only support the possible involvement of some previously reported genes and

pathways in COPD pathogenesis but also suggest some novel potential pathways.

16

GENE EXPRESSION PROFILING IN COPD

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21

Figure Legends: Figure 1. A flow diagram showing the selection of subjects/samples for gene expression

profiling. See methods for details.

Figure 2. FEF 25-75 % P was significantly related to pack-years smoked for the whole

group. FEF 25-75 % P = 92.84 – 0.71*pack-years (r=0.50, p<0.0005).

Figure 3 A heatmap of the 203 genes whose level of expression was significantly related

to FEF 25-75 % P. The relative expression for each transcript is shown for each of the 48

subjects. Cyan indicates a transcript that is under-expressed relative to the reference pool

and magenta a transcript that is over-expressed. The intensity of the color is related to the

log fold difference between the reference and the subject for that transcript (-0.6-+0.6 log

range). Each row represents one subject and their status (Non smoker, GOLD 0, etc) is

shown at the right of the row. The subjects are ordered from highest to lowest FEF 25-75

% P as illustrated by the red symbols on the left of the plot. The transcripts are shown in

order of the strength of their relationship to FEF 25-75 % P and the correlation coefficient

for the relationship is shown as the blue line ranging between -1 and +1 at the top of the

plot. Transcripts on the left side of the figure are relatively over-expressed in obstructed

subjects while transcripts on the right side are over-expressed in non obstructed subjects.

Figure 4. The relationship between FEF 25-75 % P and the level of expression of a

PLAUR. PLAUR expression = 0.06 -0.0063 FEF 25-75 % P. (r= -0.44, P ~ 0.001).

Figure 5. Representative images of PLAU and PLAUR immunohistochemisty.

A. PLAUR stained alveolar macrophages. B. PLAU stained alveolar macrophages

C. Negative control. D. PLAUR stained airway epithelium. E. PLAU stained airway

epithelium. F. Negative control. Magnification: 240X

Figure 6. A section of lung parenchyma showing multiple individual polymorphonuclear

leukocytes which stain positively for PLAUR (black arrows). Magnification 100X oil

immersion.

Figure 7. . Ingenuity Pathways Analysis of a sample of the signature genes. Genes in red

and green are up-regulated and down-regulated signature genes, respectively, and the

numbers associated with the gene names are average fold changes between the GOLD 0

reference pool and the GOLD 2 subjects. This pathway represents the first network

retrieved from the Ingenuity Pathways Analysis tool after loading the 203 signature gene

set. The net results of up-regulated urokinase plasminogen activator (PLAU), urokinase

plasminogen activator receptor (PLAUR), and thrombospondin 1 (THBS1) could be an

increase of both matrix metallopeptidase 9 (MMP9) and transforming growth factor beta

1 (TGFβ1) activities whereas the attenuated secretoglobin 1A1/uteroglobin (SCGB1A1)

and increased prostaglandin-endoperoxide synthase 2 (PTGS2/COX-2) expression could

potentially result in enhanced inflammatory response. Many genes in this network are

associated with apoptosis pathways including; TNF receptor superfamily member 6

(FAS), epithelial growth factor receptor (EGFR), brain-derived neurotrophic factor

(BDNF), phospholipid scramblase 1 (PLSCR1), sphingolipid G-protein-coupled receptor,

5 (EDG5), homeodomain interacting protein kinase 2 (HIPK2), forkhead box O3A

(FOXO3A), BCL2-associated X protein (BAX), tyrosine 3-monooxygenase/tryptophan

5-monooxygenase activation protein (YWHAH) and tyrosine 3-

monooxygenase/tryptophan 5-monooxygenase activation protein (YWHAZ) . Other

genes shown in this network include Coxsackie virus and adenovirus receptor (CXADR),

gastrin (GAS/GAST), ret proto-oncogene (RET), calcium modulating ligand (CAMLG),

homer homolog 1 (HOMER1), GRB2-associated binding protein 1 (GAB1), and DnaJ

(Hsp40) homolog subfamily B1 (DNAJB1). Please refer to

https://analysis.ingenuity.com/ for details concerning the meaning of shapes and lines

shown in the figure.

GOLD STAGE N AGE (yrs)

MALE/ FEMALE

PK YEARS

FEV1% PREDICTED FEV1/FVC %

FEF 25-75 % P

DIAGNOSES

NON-

SMOKERS 5 48±8 3/2 O 92±3 82±1 100±15 5 Carcinoid

0 21 65±2 13/8 36±4 99±3 76±1 90±4

6 Squamous 10 Adeno

4 Large cell 1 Small cell

1 9 63±4 6/3 51±5 90±3 65±1 50±3

4 Squamous 3 Adeno

1 Large cell 1 Small cell

2 10 64±3 7/3 51±7 66±2 58±1 28±2

1 Squamous 5 Adeno

1 Large cell 2 Poorly differentiated

1 Amyloid

3 3 67±5 2/1 49±7 46±0.1 53±5 19±1

1 Adeno (BAC)

1Squamous 1Carcinoid

Table 1. Subject characteristics. Means ± SE. An ANOVA showed no significant difference in age by GOLD category but significant differences for pack years, FEV1% predicted, FEV1/FVC% and FEF 25-75% predicted. (p< 0.0001 for all comparisons) Among smokers there was no significant difference in pack years by GOLD category. GOLD 0 – 14/21 Current smokers, GOLD 1 - 6/9 Current smokers. GOLD 2 – 9/10 Current smokers and GOLD 3 – 2 Current smokers and one status unknown. Current smoker = within 6 months of surgical resection.

Table 2: Immunostaining for PLAU and PLAUR in Bronchiolar epithelium and alveolar macrophages.

BRONCHIOLAR EPITHELIAL

STAINING ALV EOLAR MACROPHAGE

STAINING

PLAU % Positive

PLAUR % Positive

PLAU % Positive

PLAUR % Positive

FEF 25-75 % P <60% 89 ±28 90 ± 16 68 ± 33 69 ± 23 FEF 25-75 % P >60% 80 ±22 69 ± 32 68 ± 32 65 ± 26 P Value 0.261 0.033 0.96 0.66

Agilent microarray gene profiling

Quantitative real-time RT-PCR

731patients who gave consent and had lung function and lung/lobar resection

185 patients whose lung tissue was frozen at -80oC

• Quantitative morphometric analysis • Immunohistochemical validation

Figure 1

74 patients with a range of lung function

48 patients whose mRNA passed QC

Figure 2

SNelson

Figure 3

0 50 100 150-0.6

-0.5

-0.4

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

FEF 25-75 %P

PLA

UR

PLAUR = 0.06 - 0.0023 * FEF 25-75 %P

Figure 4

Figure 5

A

FE

CB

D

Figure 6

Figure 7

ONLINE DATA SUPPLEMENT

Gene Expression Profiling in the Lungs of Patients with Chronic Obstructive

Pulmonary Disease (COPD) and Lung Cancer

I-Ming Wang, Sergey Stepaniants, Yves Boie, James R Mortimer, Brian Kennedy,

Mark Elliott, Shizu Hayashi, Leanna Loy, Silvija Coulter, Sandra Cervino, Jennifer

Harris, Michele Thornton, Richard Raubertas, Chris Roberts, Jim C Hogg , Michael

Crackower, Gary O’Neill and Peter D Paré

ONLINE SUPPLEMENT

ONLINE SUPPLEMENT: Tissue processing: Immediately following resection, the lung or lobe was obtained from

the operating room and after the clinical specimens of the lesion, lymph nodes and the

resection margin were obtained the lobes and lungs were inflated using a 50% mixture of

CryomatrixR and saline and frozen in liquid nitrogen fumes. The frozen lungs and lobes

were then cut into 7-15 two cm thick slices using a band saw and multiple randomly

stratified blocks were acquired (1-3/slice) using a power driven hole saw fitted with a 1.5

cm diameter bit. The frozen “cores” were stored at -80o C for later cryosectioning and

RNA extraction. Prior to RNA extraction the blocks were removed from the freezer and

while on dry ice were cut in half with one half of the core being used for RNA extraction

and one half for frozen sections for quantitative morphometery and

immunohistochemistry.

Frozen sections were obtained from the surface of the ½ core immediately

adjacent to the portion used for mRNA extraction. The 10 micron sections were stained

with hematoxylin and eosin and a digital image of the entire sample was captured. The

fraction of the tissue area occupied by air and tissue was determined and the tissue

content was further divided into alveolar tissue, membranous airway, cartilaginous

airway and blood vessel tissue using a standard point counting method. (E1 )

RNA processing and Quality Control: Homogenization of the lung cores for RNA

extraction was performed in 10 ml of Trizol reagent. Phase separation and RNA

precipitation were performed according to reagent protocol. RNA pellets were dissolved

in 100 ul of RNase-free water. The Qiagen RNeasy Mini Protocol for RNA cleanup was

then followed for further RNA cleanup and on-column DNase digestion. Elution steps

were performed twice to maximize RNA recovery.

Assessment of sample concentration and integrity are essential for ensuring the quality of

expression data. A fixed volume (5 µL total) of each total RNA sample was used to

determine both sample concentration and integrity. Samples concentrations were assayed

by determining the OD260 by UV spectrophotometer, with a passing concentration range

set at >0.11 ug/ul. Samples whose concentrations exceed the upper limits of the

amplification protocol were diluted to a target concentration of 0.2 ug/ul and re-assayed

to confirm accurate dilution. RNA sample integrity was evaluated by calculating the

1

ONLINE SUPPLEMENT

rRNA ratio of 28S/18S using the Agilent Bioanalyzer capillary electrophoresis system.

The passing criteria for use in RNA microarray experimentation was a 28S/18S rRNA

ratio between 0.75 - 3.02. Samples passing RNA sample QC were released for

subsequent amplification and hybridization.

As an array is scanned in the Agilent DNA Microarray Scanner carousel, a TIFF image is

produced revealing the fluorscence from the Cy3 (570 nm) and the Cy5 (670 nm)

channels. Several quality metrics were used to assess the quality of the microarray and

the quality of the RNA. These metrics provide guidance into in probable causes of failure

and likelihood of correction upon repeat. Potential failures include inefficiencies in

amplification or subsequent processing, or physical defects on the array features. Profile

QC metrics are based on numerous criteria including co-amplified synthetic spike-ins,

full array summary statistics, array manufacturing assessment gridline spike-ins, and

individual feature assessment. All QC metrics were evaluated through an automated

process and displayed in Resolver. By default, metrics which indicate defective arrays or

array processing were automatically recoupled, whereby a second aliquot of the amplified

cRNA samples was coupled to Cy dyes, then combined and hybridized to a new array.

cRNA Labeling and Expression Profiling. cDNA was produced from 5 μg total RNA by

reverse transcription (RT) using Moloney murine leukemia virus (MMLV) RTase and

then transcribed into cRNA by in vitro transcription (IVT) using T7 RNA polymerase. 5-

(3-Aminoallyl)uridine 5V-triphosphate (Sigma) was incorporated into cRNA in the IVT

reaction. For cRNA labeling, the allylamine-derivatized cRNA products were reacted

with N-hydroxysuccinimide esters of Cy3 or Cy5 dyes (Amersham Pharmacia Biotech,

Piscataway, NJ) as described previously (E 2). The resulting labeled probes were

hybridized to hu25k oligonucleotide microarrays. All hybridizations were done in

duplicate with fluor reversal on two microarrays to compensate for potential biases due to

the different chemical properties of Cy3 and Cy5 dyes. The arrays were scanned to detect

the level of gene expression for 21,000 genes as described previously (E3). Fluorescence

intensities of scanned images were quantified, normalized and corrected to yield the

transcript abundance of a gene as an intensity ratio with respect to that of the signal of the

reference pool.

2

ONLINE SUPPLEMENT

Microarray study design and methodology: Figure E1 shows the design of the

microarray experiment. RNA from 8 of the 21 GOLD 0 subjects (non-obstructed

smokers) was pooled to form the reference RNA. For 2-color array experiment, a

reference pool with sufficient RNA to compare with each individual sample within the

study (including samples used as the reference pool) is necessary. This pool forms the

“control”. The amount of RNA from each sample to be included in the reference pool

needs to be the same to keep this pool balanced. We chose smokers who did not develop

airflow obstruction (GOLD 0) as our “control”. We tried to include as many GOLD 0

samples in the pool as possible and the 8 samples chosen are those on whom we had

sufficient RNA to contribute to the pool and still have enough RNA to be compared back

to the pool.

The reverse transcribed cDNA from this reference was used for competitive

hybridization against all of the other samples including all of the GOLD 0 samples which

made up the reference pool. Microarray profiling was done as previously described (E-

4). The two-colour microarrays were scanned using the Agilent scanner and proprietary

image acquisition software. Rigorous image QC using proprietary software was

performed. The raw data were warehoused in a proprietary database. Experimental QC

was performed in MATLAB (Mathworks, Inc. Natick, MA, http://www.mathworks.com).

At this point, spiked-in exogenous mRNA hybridizations were examined for a large

number of known problems and attempts were made to explain any abnormal trends or

outlier arrays. Expression data were loaded into the Resolver (Rosetta proprietary

software database for transformation, http://www.rosettabio.com/

products/resolver/default.htm), normalization and error modeling. Fluor-reversed pairs

for each sample were combined to give a single log-ratio and a p-value for technical

variability for each biological sample compared to its appropriate control. Next, 1D and

2D clustering and classifier analysis was used to get an overview of the experiment. Data

mining of these clusters was performed using prior biological knowledge, known

pathways, and gene ontology or keyword over-representation. Data was exported to

3

ONLINE SUPPLEMENT

Spotfire/decisionsite for further analysis and visualization (Spotfire, Somerville MA,

http://spotfire.com).

TaqMan validation: A number of the genes whose level of expression proved to be

related to the level of lung function (COPD signature genes) were tested using TaqMan

real-time PCR. Table E1 and Figure E2. The level of gene expression for selected COPD

signature genes was measured with quantitative real-time PCR utilizing commercially

available TaqMan assays (Applied Biosystems). PCR primers in these assays are

designed to span introns to minimize potential problems from genomic DNA

contamination. All measurements were made in triplicate using the TaqMan Universal

PCR Master Mix (Applied Biosystems). Relative expression results were obtained from

the ratio of the mean of triplicate target gene measurements to the mean of triplicate

house-keeping gene measurements. qPCR results represent the fold change for each gene

in each subject compared to the level in the pooled reference sample.

Immunohistochemistry: Frozen human lung specimens were cut at 10 µm thickness

onto slides and stored at -70°C until used. For PLAUR and thrombospondin staining the

APAAP method (E5) was used while for PLAU the ABC method (DAKO Handbook)

was employed. (E6) Briefly, sections were thawed at room temperature, and fixed for 10

minutes in 10% neutral buffered formalin. Slides were rinsed in water then with Tris-

buffered saline (TBS, pH 7.6). Sections were blocked with Universal Protein Block

Serum-Free (DAKOCytomation, Mississauga, ON) and incubated at room temperature

for 20 minutes before application of primary antibody. All antibodies were diluted in 1%

bovine serum albumen (BSA, Sigma, St. Louis, MO) in TBS. Primary antibodies were

incubated overnight at 4°C in a humid chamber. Mouse monoclonal antibody against

PLAUR, clone IID7 (10ug/mL Santa Cruz,CA), rabbit polyclonal antibody against

PLAU, clone H-140 (10ug/mL Santa Cruz,CA), and mouse monoclonal antibody against

thrombospondin 1, clone A6.1 (40ug/mL Lab Vision, Fremont, CA) were used. For

negative controls, isotype matched mouse non-immune immunoglobulins (BD

Biosciences Pharmingen, Mississauga, ON) or Rabbit Immunoglobulin Fraction

(DAKOCytomation) replaced the primary antibody. Non-small cell lung carcinomas

were used as positive control tissue for PLAU and PLAUR staining. Tonsil was used as

positive control tissue for thrombospondin staining.

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ONLINE SUPPLEMENT

The following morning all slides were rinsed for 20 minutes in TBS on an orbital shaker

at a speed of 100 RPM to reduce non-specific binding of primary antibody.

For PLAUR and thrombospondin staining, rabbit anti-mouse IgG (DAKOCytomation)

was added to slides and incubated for 30 min followed by a 10 min rinse in TBS.

Subsequently, APAAP mouse monoclonal antibody (DAKOCytomation) was added to

slides and incubated for 30 min and then rinsed for 10 min in TBS.

For PLAU staining, biotinylated goat anti-rabbit IgG (DAKOCytomation) was added to

slides and incubated for 30 min followed by a 10 min rinse in TBS. Subsequently, ABC

complex (DAKOCytomation) was added to slides and incubated for 30 min and then

rinsed for 10 min in TBS.

All slides were visualized using Naphthol/New Fuschin as chromogenic substrate

(DAKO Handbook). After immunohistochemical staining, slides were counterstained

with Gill’s hematoxylin (Sigma), dehydrated and coverslipped.

Image Analysis: For PLAU and PLAUR, all images were captured using a Nikon

Eclipse E600 microscope fitted with a SPOT camera. The image analysis was performed

using Image-Pro Plus 4.0 (Media Cybernetics, Silver Spring, MD). Alveolar

macrophages positive for PLAU and PLAUR were expressed as a percent of total

macrophages and the length of the membranous bronchiolar epithelium which stained

positively for these proteins was expressed as a percent of the total epithelial length.

Only selected sections were stained for thrombospondin.

The histological sections were examined by observers who were blinded to all

information except that present on the slide. From each slide, 3 random fields were

sampled. To quantify PLAU/PLAUR staining in alveolar macrophages the numbers of

positive and negative cells were determined by counting cells from images taken at 120x

magnification. The total positive cells per high power field and the % of alveolar

macrophages positive for PLAU and PLAUR were calculated. Positive airways were

determined by any positive staining in any airway compartment and quantified as the

length of positive staining/length of airway epithelium expressed as a percent. The extent

of PLAU and PLAUR staining was compared to FEF 25-75 % P and Pack Years using

linear regression analysis and in addition the mean % positive macrophages and %

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ONLINE SUPPLEMENT

positive airway epithelial length in subjects who had a FEF 25-75 % P <60% predicted

were compared to those who had a FEF 25-75 % P >60% predicted.

The inclusion of % parenchyma did not substantially impact the slope and p-values for

the relationships with FEF 25-75 % P suggesting that the relationship between %

parenchyma and gene expression, although strong, does not confound the relationship

between FEF 25-75 % P and gene expression. Fig E3 is a Venn diagram showing the

overlap of the number of genes which correlated with FEF 25-75 % P when % parenchyma

was, and was not, included in the model.

FEF 25-75 % P was used for comparison with gene expression data since it provided a

wider range of values than FEV1% predicted. To compare the genes which correlated

with FEF 25-75 % P and FEV1% Predicted we also ran the model using this measure of

lung function. Figure E4 is a Venn diagram showing the overlap of the number of genes

which correlated with FEF 25-75 % P and with FEV1 % Predicted.

The genes which we report to be correlated with lung function showed very little overlap

with the genes whose level of expression has been correlated with lung function in three

published reports. (E 7-9). Similar discordance is evident in a comparison between the

other studies. (Table E2). This lack of concordance could occur for a variety of reasons.

Some of the relationships in all of the studies are likely to be spurious and related to the

large number of transcripts that are profiled on very few subjects. We used stringent

criteria to limit the possibility of false positive relationships and adjusted for transcripts

whose expression was related to smoking rather than airflow limitation. It is likely that

much of the discrepancy between studies is methodologic. As shown in table E3 the

patient characteristics, disease severity and method of gene profiling were quite different

between studies.

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ONLINE SUPPLEMENT

Figure Legends:

Figure E1. The relative level of expression of all of the genes whose expression was

assayed by TaqMan and microarray is shown. The equation for the relationship is:

Microarry value = 0.69 TaqMan value + 0.48 (R2 = 0.63). See table E1.

Figure E2. Experimental design for microarray analysis. RNA from 8 non-obstructed

smokers (GOLD 0) was pooled as the reference. Gene expression in all 48 samples was

compared to this reference.

Figure E3. Venn diagram showing the overlap of FEF 25-75 % P related genes in a model

which did not, and did, incorporate % parenchyma.

Figure E4. Venn diagram showing the overlap of genes related to FEF 25-75 % P and to

FEV1%Predicted in independent models. Of the 203 most highly associated genes using

FEF 25-75 % P 67 were among the 187 most highly associated using FEV1% Predicted.

ONLINE SPREAD SHEETS: (see separate Excel files)

A. An excel spread sheet listing the 203 signature COPD related genes and their fit to

the model are listed in order of the lowest to highest p value for the relationship

between gene expression and FEF 25-75 % P.

B. An excel spread sheet listing all transcripts whose level of expression was

positively (r ≥ + 0.3) or negatively (r ≥ - 0.3) related to the % of the sample made

up of lung parenchyma.

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References: E1. Coxson HO, Hogg JC, Mayo JR, Behzad H, Whittall KP, Schwartz DA.

Quantification of idiopathic pulmonary fibrosis using computed tomography and histology. Am J Respir Crit Care Med 1997; 155: 1649-1656.

E2. Hughes TR, Mao M, Jones AR, et al. Expression profiling using microarrays

fabricated by an ink-jet oligonucleotide synthesizer. Nat Biotechnol, 2001;19:342 – 7.

E3. van’t Veer LJ, Dai H, van de Vijver MJ, et al. Gene expression profiling predicts

clinical outcome of breast cancer. Nature, 2002;415:530 – 6. E4. Lampe, Johanna W., Stepaniants, Sergey B., Mao, Mao, Radich, Jerald P., Dai,

Hongyue, Linsley, Peter S., Friend, Stephen H., Potter, John D. Signatures of Environmental Exposures Using Peripheral Leukocyte Gene Expression: Tobacco Smoke. Cancer Epidemiol Biomarkers Prev 2004 13: 445-453

E5. Cordell, JL, Falini, B, Erber, WN, Ghosh, AK, Abdulaziz, Z, MacDonald, S, Pulford,

KAF, Stein, H and Mason, DY. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Hisotchem. Cytochem. 32: 219-229, 1984.

E6. DAKO Handbook for Immunochemical Staining Methods, Naish, SJ (ed). DAKO

Corporation, Carpenteria, CA. 1989. E7. Spira A, Beane J, Pinto-Plata V, Kadar A, Liu G, Shah V, Celli B, BrodyJS. Gene

expression profiling of human lung tissue from smokers with severe emphysema. Am J Respir Cell Mol Biol 2004;31:601–610.17.

E8. Golpon HA, Coldren CD, Zamora MR, Gosgrov GP,Moore MD, TuderRM, Geraci

MV, Voekel NF. Emphysema lung tissue gene expression profiling. Am J Respir Cell Mol Biol 2004;31:595–600.

E9. Ning W, Li CJ, Kaminski N, Feghali-Bostwick CA, Alber SM, Di YP,Otterbein SL,

Song R, Hayashi S, Zhou Z, et al. Comprehensive gene expression profiles reveal pathways related to the pathogenesis of chronic obstructive pulmonary disease. Proc Natl Acad Sci USA 2004;101:14895–14900.

8

Table E1: Microarray and Taqman Results

Gene Patient # qPCR Array 2425 2.84 2.06 2427 1.89 1.54 3040 2.46 1.28 BMPR2

6280 0.92 1.82 2086 2.32 2.21 2131 6.67 4.16 2427 2.57 2.61 3385 2.09 2.08 6191 1.74 1.82 3380-14 1.57 1.31

TSP1

3492-6 2.54 1.33 2076 2.03 1.74 2086 1.28 1.42 2131 2.30 1.77 2427 2.82 2.46 3404 1.31 1.29 3380-14 0.70 0.71

PLAUR

3492-6 2.46 1.25 2131 1.20 1.36 3404 1.38 1.38 PLAU 6191 1.51 1.76 2427-12 3.90 4.23 3380-14 1.43 1.89 CXCL1 3492-6 5.60 3.12 2427-12 5.17 7.05 3380-14 0.64 0.68 IL-8 3492-6 3.37 2.05 2427-12 2.66 3.73 3380-14 0.48 0.69 CCL2 3492-6 2.94 2.30 2427-12 0.32 0.63 3380-14 0.67 1.23 MMP9 3492-6 1.13 1.53

Table E1. The level of expression of each gene for each of the subjects relative to the level of expression in the pooled sample is shown. Although the level of gene expression by TaqMan is systematically greater there is excellent concordance between the methods. See Figure E1.

Study Current study Spira et al Golpon et al Ning et al Current study 203 Spira et al 1 102 Golpon et al 0 1 74 Ning et al 1 5 3 325

Table E2: A comparison of the overlap of differentially expressed genes in 4 COPD gene expression studies. The gene which was common to the current and Spira’s study (E7) was RAB1A*; the gene which was common to the current and Ning’s study (E9) was CDKL1; the 5 genes common to the Ning and Spira studies were C1R*, C3, HSPA8, SDC1* and TPM2*; the 3 common to the Golpon (E8) and Ning studies were ALCAM, GYPC and SET*; the single gene common to the Golpon and Spira studies was TOMM20. Genes indicated with an asterisk were differentially expressed in the same direction (ie up or down in both studies)

Study Current Study Spira et al Golpon et al Ning et al

Category Diseased

(Gold 1,2&3)

Normal Gold 0&

non-smokers

Severe Emphysema

Mild/No Emphysema

Emphysema AAT

deficiency

Normal non-

smokers Gold 2 Gold 0

No of Patients 22 26 18 12 5

6 5 14* 12*

Age (yrs) 64 62 64 69 61 51 50 63 63

Female/Male 7/15 10/16 10/8 4/8 1/ 4 1/ 5 3/2 ND ND

Pk Years (±SD) 51(18) 29(22) 61(31) 43(30) 34(4)

14(8) 0 40(19) 31(15)

FEV1 % predicted

(±SD) 67(18) 95(12) 21 (6) 65 (12) ? ? 64 (7) 102 (13)

Platform Agilent Affymetrix Affymetrix

SAGE Incyte cDNA Array

Table E3: A comparison of gene expression studies in COPD. There are differences in the number, age, gender, smoke exposure and severity of lung disease in the COPD and control groups. In addition different gene expression platforms were used. * indicates that the mRNA from some of these subjects was pooled. . ND = gender was not reported.

Table E 4: The number of genes whose expression related to FEF 25-75 % P (overlap) in different GO Molecular and Biological Function Pathways relative to the number of genes in the pathways (Set Gene)

12

0

2

4

6

8

0 2 4 6 8TaqMan values of gene expression

Mic

roar

ray

valu

es fo

r gen

e ex

pres

sion

Figure E 1

Ref. Pool, N=8

GOLD 1-1

Non-Smokers(NS) N=5

GOLD 0N=21

GOLD-1 N=9GOLD-2 N=10GOLD-3 N=3

GOLD 0-1

GOLD 0-2

GOLD 0-3

GOLD 0-4

GOLD 0-5

GOLD 0-6

GOLD 0-7

GOLD 0-8

GOLD 0-2 GOLD 0-3 GOLD 0-21

GOLD 1-2

GOLD 1-3

GOLD 1-9

GOLD 2-1

GOLD 2-2

GOLD 2-3

GOLD 2-10

GOLD 3-1

GOLD 3-2

GOLD 3-3

NS1

NS2

NS3

NS4

GOLD 0-1

NS5

Figure E 2

Without Parenchyma With Parenchyma

101102 31

Genes in which the FEF 25-75 and whole model p values were 0.02

Figure E 3

Venn diagram of gene selection using Post Bronchodilator FEF 25-75% P and Post Bronchodilator FEV1% P without parenchyma

FEF 25-75%P without parenchyma FEV1%P without parenchyma

67136 120

Gene set 1: FEF 25-75 p < 0.02 & Model p < 0.02 = 203 genesGene set 2: FEV1%P p < 0.05 & Model p < 0.02 = 187 genes

Figure E 4

SPREAD SHEET A: THE 203 COPD SIGNATURE GENES, RANKED IN ASCENDING P VALUES FOR RELATIONSHIP WITH FEF 25-75%P

Sequ

ence

cod

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gene

nam

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inte

rcep

t

inte

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FEF

25-7

5%P

slop

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FEF

25-7

5% p

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pack

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nt

Contig53260_RC LOC196264 0.2755 4.39E-06 -0.002302 2.20E-05 -0.001981 0.005774 9.94E-05 -0.5554NM_016371 HSD17B7 -0.3253 5.05E-08 0.002052 4.72E-05 0.002813 7.38E-05 3.43E-05 0.3653NM_016013 CIA30 0.1628 0.002132 -0.002053 5.89E-05 -0.000843 0.2012 0.0001896 -0.3808Contig47222_RC GPI 0.2212 2.28E-05 -0.001713 0.000251 -0.001198 0.05428 0.001133 -0.5318AK027027 AK027027 -0.1908 0.00398 0.002257 0.0003382 0.001195 0.151 0.001328 -1.205NM_013368 RBT1 0.1823 0.001236 -0.001885 0.0003637 -0.000985 0.1587 0.001411 0.1527NM_015523 DKFZP566E144 0.2003 0.0001201 -0.00169 0.0003662 -0.001176 0.06305 0.00162 0.03845AL137597 C20orf110 0.2654 1.75E-05 -0.001944 0.000396 -0.001963 0.008755 0.001267 -0.8477Contig51675_RC -0.1144 0.02498 0.001743 0.0004179 0.001161 0.07797 0.001823 -1.045Contig48542_RC C1GALT1 0.1727 0.03177 -0.002728 0.0004658 -0.000604 0.555 0.0008747 -1.198ENST00000275206 ENST00000275206 -0.2055 7.46E-05 0.001629 0.0004973 0.000768 0.215 0.001764 -0.05148NM_004961 GABRE 0.3473 6.68E-07 -0.002054 0.0005324 -0.003038 0.000319 0.0003218 -1.244NM_016346 NR2E3 -0.118 0.05431 0.002047 0.0005903 0.001626 0.04279 0.002486 -1.276AL137403 -0.2503 0.0005999 0.002268 0.0007329 0.001206 0.1777 0.002796 -1.102NM_000935 PLOD2 0.23 0.002162 -0.00236 0.000757 -0.001772 0.06036 0.003199 -1.275NM_000805 GAS -0.2248 0.004221 0.00246 0.0008706 0.0015 0.1292 0.003543 0.02776NM_032044 REG4 -0.1473 0.03048 0.002177 0.0008808 0.001453 0.09814 0.003682 -1.482NM_000963 PTGS2 0.4696 0.001941 -0.00468 0.0009122 -0.004468 0.02038 0.003165 -0.2248Contig6164_RC -0.2855 0.00384 0.003067 0.0009359 0.00174 0.1602 0.003665 0.1098NM_003637 ITGA10 -0.1292 0.058 0.002176 0.000958 0.001031 0.24 0.00334 -1.442Contig25228_RC -0.4985 0.0001284 0.003872 0.000959 0.003481 0.02909 0.003586 -0.765NM_018847 KIAA1354 0.2807 2.89E-06 -0.001698 0.0009794 -0.002052 0.004177 0.001876 -0.8194

NM_006541 TXNL2 -0.4108 4.34E-06 0.00254 0.0009946 0.001526 0.1399 0.003991 -1.126NM_014182 ORMDL2 -0.3636 3.95E-06 0.002227 0.001037 0.002098 0.02356 0.003648 -0.6231NM_134431 SLC21A3 0.4348 2.33E-05 -0.002958 0.001078 -0.004015 0.001626 0.001243 -1.435NM_018429 BDP1 -0.3469 6.98E-05 0.002543 0.001089 0.001953 0.06348 0.004477 -0.58NM_178542 DKFZp762C2414 -0.2227 3.12E-05 0.001537 0.001141 0.001192 0.06207 0.004663 0.07653NM_004606 TAF1 -0.1838 0.002317 0.001826 0.001142 7.18E-05 0.9231 0.001189 -1.076NM_182568 FLJ36492 -0.1468 0.04189 0.002253 0.001165 0.001668 0.07494 0.004805 -0.5187NM_006145 DNAJB1 0.2899 2.74E-05 -0.001972 0.001202 -0.002914 0.000769 0.000858 -0.2545NM_170734 BDNF 0.5213 0.0001586 -0.004024 0.001205 -0.005317 0.002315 0.00161 -1.113ENST00000271276 FLJ37099 -0.2446 0.01329 0.002987 0.001412 0.001451 0.2465 0.004933 -0.579NM_021244 RRAGD -0.3195 3.90E-05 0.002189 0.001424 0.002582 0.00676 0.003016 0.516AL137315 LOC116068 0.1916 0.01642 -0.002413 0.001456 -0.00199 0.05327 0.005706 -0.6415NM_006661 PDE10A -0.09264 0.09658 0.001712 0.001492 0.00084 0.2446 0.005232 -0.901NM_033130 SIGLEC10 -0.3098 0.0004816 0.002566 0.001499 0.003182 0.004785 0.002668 -1.104NM_003246 THBS1 0.3122 0.01539 -0.003825 0.00171 -0.000961 0.5544 0.003711 -0.07766NM_015936 CGI-04 -0.2125 1.15E-05 0.001314 0.001771 0.0009 0.1129 0.007101 0.2604Contig54718_RC FLJ37562 0.2231 0.001401 -0.002007 0.001794 -0.0016 0.06703 0.007054 -0.4272NM_148894 FLJ33215 -0.3022 0.0001232 0.002193 0.001819 0.001041 0.2697 0.006192 -0.5864BC010117 BCAN -0.2118 0.01317 0.002504 0.001896 0.001719 0.1148 0.007568 -0.5961AK000942 -0.3016 1.43E-05 0.001875 0.001918 0.00115 0.1581 0.007451 -0.09722NM_006130 BMP1 -0.219 0.01757 0.002706 0.001943 0.001202 0.3045 0.006308 -0.8919NM_138287 LOC151636 -0.2592 0.008999 0.002884 0.001973 0.002516 0.04802 0.00731 1.237AK025156 AK025156 -0.1652 0.03067 0.002252 0.001979 0.000987 0.3118 0.006365 -0.9074AK021682 COL9A2 -0.1458 0.05701 0.00227 0.00199 0.001798 0.07174 0.00779 -0.9858NM_002148 HOXD10 -0.1099 0.07806 0.001854 0.001992 0.00119 0.1415 0.007827 -0.8439Contig16694_RC LOC150297 -0.2491 0.0209 0.003157 0.002014 0.001398 0.3078 0.006518 -0.7827NM_004075 CRY1 0.2017 0.003741 -0.001996 0.002025 -0.002747 0.0026 0.002356 -0.9231NM_006186 NR4A2 0.4997 0.0026 -0.004716 0.002129 -0.003906 0.0619 0.008127 -0.2252NM_003405 YWHAH 0.1062 0.1309 -0.002085 0.002129 -0.000461 0.6112 0.004294 -0.08724NM_139131 NUP98 0.1914 0.001379 -0.001682 0.002195 -0.000905 0.221 0.007974 -0.04812AK024868 ELKS -0.3295 2.21E-07 0.001601 0.002208 0.001609 0.02541 0.006862 -1.161NM_017954 CADPS2 -0.1124 0.04361 0.001627 0.002212 0.001029 0.1519 0.008593 0.2073NM_004230 EDG5 -0.2862 0.001157 0.002468 0.002218 0.002142 0.05196 0.008193 0.1918NM_054028 AMAC -0.1373 0.01725 0.001666 0.002233 0.000305 0.6748 0.004064 -1.044Contig52994_RC 0.2757 0.00131 -0.002404 0.00225 -0.002759 0.01155 0.005239 -0.3439NM_018608 PRO1905 -0.07234 0.1559 0.001505 0.002254 0.000964 0.1477 0.008777 -1.324NM_000165 GJA1 0.2857 0.001924 -0.002587 0.002295 -0.00324 0.006322 0.00405 0.3434

NM_000651 CR1 -0.4495 1.19E-06 0.002365 0.002344 0.00352 0.001458 0.001823 -1.497AL133611 ARL8 0.296 0.003616 -0.002868 0.002364 -0.002418 0.06039 0.008871 -0.1646Contig29287_RC SIMP -0.1968 0.0008315 0.001627 0.002433 0.000624 0.386 0.007093 -0.02042NM_001550 IFRD1 0.2044 0.007882 -0.002182 0.002436 -0.001042 0.2819 0.008214 -0.4777NM_001745 CAMLG 0.2453 3.00E-05 -0.001558 0.002446 -0.001568 0.0268 0.007561 -0.03362Contig31461_RC FLJ11457 -0.1743 0.03744 0.002413 0.002466 0.000686 0.5206 0.005855 0.00333Contig57908_RC C6orf145 0.2342 2.48E-05 -0.001461 0.002569 -0.001835 0.006779 0.004514 -0.8648NM_004004 GJB2 -0.1381 0.04921 0.002018 0.002599 0.001393 0.1252 0.01014 -1.028Contig12432_RC -0.1058 0.05111 0.001555 0.002652 0.001192 0.09033 0.01027 -1.407AK057728 FLJ10276 -0.1398 0.03545 0.001895 0.002696 0.000385 0.6486 0.005241 -0.9963ENST00000257780 ENST00000257780 -0.2803 0.00609 0.002851 0.002738 0.002287 0.078 0.01043 -0.1298Contig46591_RC -0.1782 0.0006773 0.001422 0.002813 0.000939 0.1456 0.01085 -0.01965ENST00000218091 ENST00000218091 -0.15 0.0534 0.002208 0.002855 0.001247 0.2124 0.01045 -0.6339NM_030881 DDX17 -0.5747 0.0009728 0.00473 0.00289 0.003076 0.1529 0.01109 0.3193RSE_00000869470 olfactory receptor -0.2192 0.001257 0.001849 0.002908 0.001727 0.04283 0.009876 -0.2616Contig1254_RC RANBP2 0.2388 0.0002272 -0.001725 0.002926 -0.002093 0.009679 0.005827 0.0857AB015616 12MelaCE5B3CD -0.148 0.02743 0.001887 0.002979 0.001515 0.08041 0.01127 -1.419NM_181536 PKD1L3 -0.1213 0.07223 0.001914 0.003006 0.001399 0.1108 0.01161 -0.7304AL137619 DKFZp434G0812 -0.1928 0.02186 0.00234 0.003175 0.001949 0.07228 0.01176 0.4146Contig36809_RC -0.3738 4.56E-05 0.002368 0.003211 0.00106 0.3278 0.01027 -0.8161NM_006900 IFNA13 -0.17 0.0321 0.002212 0.003235 0.002067 0.04522 0.01092 -1.311NM_005321 HIST1H1E -0.3406 0.000135 0.002324 0.003363 0.001441 0.1798 0.0126 -1.412NM_152829 TES 0.1351 0.06409 -0.00204 0.003365 -0.001286 0.173 0.01267 -1.13NM_032009 PCDHGA2 -0.1094 0.1032 0.001883 0.003418 0.000708 0.4136 0.009757 -1.245NM_005655 TIEG 0.272 0.01585 -0.003098 0.003446 -0.002746 0.0586 0.01218 0.2298ENST00000298035 LOC220213 0.2889 0.007246 -0.002926 0.003472 -0.003359 0.01587 0.008143 -0.6167NM_173172 NR4A2 0.4482 0.005454 -0.004366 0.00352 -0.003523 0.0848 0.01313 -0.3028NM_018054 RICH1 -0.3099 0.0001661 0.002136 0.00353 0.001371 0.1679 0.0133 0.04821NM_138775 LOC91801 -0.287 0.0001927 0.001999 0.003576 0.000937 0.3116 0.01171 -0.8259NM_032578 MYOP -0.1183 0.1066 0.002044 0.003604 0.00159 0.09707 0.01358 -1.205NM_006804 STARD3 -0.5153 0.00138 0.00428 0.003607 0.004226 0.03704 0.01131 0.445RSE_00000772559 RSE_00000772559 -0.639 0.00059 0.004888 0.003619 0.004695 0.04235 0.01177 0.2414NM_024848 FLJ13941 -0.3509 0.001902 0.003012 0.003623 0.002929 0.03998 0.0116 0.3129NM_002503 NFKBIB -0.1884 0.04 0.00253 0.003633 0.001129 0.3359 0.01154 -0.5143ENST00000283060 DKFZP434B1727 -0.1316 0.1078 0.002277 0.003667 0.001332 0.2099 0.01338 -0.6416NM_014086 PRO1073 -0.1631 0.02025 0.001919 0.003684 0.001884 0.03863 0.01164 0.6928ENST00000285726 ENST00000285726 -0.1688 0.08572 0.002725 0.003699 0.001713 0.1788 0.01382 -0.4096

ENST00000278009 ENST00000278009 -0.2009 0.04652 0.002773 0.003788 0.002897 0.02875 0.01085 -1.288NM_014166 VDRIP 0.04703 0.4666 -0.001812 0.003819 -0.000173 0.837 0.005598 0.1688ENST00000296311 ENST00000296311 -0.176 0.004592 0.001658 0.003879 0.000243 0.7526 0.006603 -0.5831NM_017600 DKFZp434M0331 -0.07669 0.2454 0.001838 0.003898 4.03E-05 0.9625 0.004536 -1.195Contig66028_RC RECK 0.2206 0.005392 -0.002111 0.004036 -0.00104 0.2949 0.0135 0.1614NM_004419 DUSP5 0.2117 0.06516 -0.003134 0.004112 -0.000583 0.6913 0.007804 -0.5905NM_173665 MGC34713 -0.07346 0.2306 0.001693 0.004115 0.001015 0.2052 0.01503 -0.9984NM_017884 PINX1 -0.1653 0.02657 0.002014 0.004149 0.001274 0.1819 0.01539 -0.1311Contig46525_RC FLJ22490 -0.06832 0.2264 0.001558 0.00417 -0.001093 0.1399 0.0001748 -0.1537NM_018554 B3GNT1 0.1307 0.1849 -0.002713 0.004193 -0.00161 0.2107 0.01524 -0.7627ENST00000293782 ENST00000293782 -0.1767 0.04296 0.002366 0.004202 0.001164 0.2978 0.01401 -0.4528ENST00000301682 ENST00000301682 -0.5027 0.002778 0.004419 0.004204 0.004262 0.0451 0.01349 -0.1791NM_138336 LOC150678 -0.1839 0.03448 0.002351 0.004226 0.001252 0.2606 0.01467 -1.183ENST00000300366 ENST00000300366 -0.3025 5.41E-05 0.001867 0.004427 0.001292 0.1482 0.01658 -0.4882Contig33882_RC -0.2308 0.008899 0.002336 0.004429 -4.44E-05 0.9679 0.00458 -1.126AF144233 PRKCBP1 -0.2804 5.14E-05 0.001722 0.004463 0.002247 0.008277 0.007123 -1.431NM_003506 FZD6 0.2198 0.009494 -0.00224 0.004522 -0.001852 0.0865 0.01638 -0.4759NM_024321 MGC10433 -0.5156 2.12E-05 0.002963 0.004709 0.002203 0.1239 0.01752 -0.07107NM_018606 PRO1787 -0.1021 0.1291 0.001818 0.004725 0.001184 0.1764 0.01745 -1.288Contig43464_RC HOMER1 0.1497 0.04674 -0.002011 0.00478 -0.001647 0.09152 0.01731 -0.8584NM_006751 SSFA2 0.1767 0.01484 -0.001911 0.004828 -0.001938 0.03873 0.0144 0.6744NM_002697 POU2F1 -0.2542 0.01545 0.002786 0.004913 0.002971 0.02872 0.01326 1.368Contig45549_RC 0.2536 0.0001502 -0.001664 0.004966 -0.001004 0.2125 0.01791 -1.467NM_032765 TRIM52 -0.2485 0.0001346 0.001616 0.004967 0.001038 0.1851 0.01822 -0.8355NM_017423 GALNT7 0.1216 0.1112 -0.002046 0.004985 0.000645 0.5123 0.001658 -0.5551NM_014897 KIAA0924 -0.3956 5.55E-06 0.002077 0.004992 0.001976 0.05261 0.01613 -1.153NM_014817 KIAA0644 0.3017 6.26E-05 -0.001852 0.00501 -0.003507 0.000284 0.0008132 -1.248NM_015544 DKFZP564K1964 0.3176 1.18E-05 -0.001744 0.005031 -0.002699 0.00235 0.003673 -0.3774NM_015387 PREI3 0.1483 0.04902 -0.002 0.005032 -0.001408 0.1479 0.01866 -0.7341Contig52494_RC SLC30A7 0.07077 0.3739 -0.002153 0.00504 -0.000194 0.8512 0.007479 -0.6124Y11162 RNU68 -0.3537 1.01E-06 0.001684 0.005072 0.002044 0.01498 0.01033 -0.852Contig58333 TRA2A -0.1053 0.1011 0.001709 0.00521 0.001261 0.1314 0.01923 -1.23NM_145174 LOC63929 -0.1583 0.08507 0.002442 0.005224 0.002011 0.09357 0.01874 -1.111NM_000043 TNFRSF6 0.1611 0.02394 -0.00187 0.005233 -0.001801 0.05125 0.01663 -0.7347NM_032876 JUB 0.3112 0.0002118 -0.002078 0.005346 -0.003687 0.000656 0.001567 0.2214NM_003120 SPI1 -0.1399 0.01897 0.001553 0.005391 0.001541 0.04566 0.01649 -1.042Contig48019_RC LIM -0.07255 0.2243 0.001594 0.005403 0.000558 0.4715 0.0146 -0.9203

AK054892 AK054892 -0.1926 0.008057 0.001875 0.005422 0.001771 0.05667 0.01756 -1.475NM_014913 KIAA0863 0.08738 0.1766 -0.001722 0.00544 -0.000188 0.822 0.008577 -0.445Contig27934_RC -0.1495 0.1225 0.002556 0.005659 0.001463 0.2441 0.01984 -0.2704NM_014570 ARFGAP3 0.06029 0.3102 -0.001578 0.00575 0.000408 0.5977 0.002569 -0.2375NM_018214 LANO 0.2322 0.0001528 -0.001495 0.005801 -0.002767 0.000463 0.001246 -0.3621NM_006401 ANP32B -0.2496 7.99E-05 0.00153 0.005806 0.001459 0.05665 0.01856 1.002AK021538 AK021538 0.2885 0.0002606 -0.001929 0.00603 -0.002515 0.01085 0.009895 -1.108Contig45782_RC DYRK3 0.3322 0.0003251 -0.002262 0.00606 -0.002312 0.04271 0.0177 -1.164Contig14375_RC -0.258 0.000577 0.001843 0.006151 0.000693 0.4471 0.01727 -0.2127Contig12814_RC -0.05702 0.4478 0.001982 0.006191 -0.000162 0.8683 0.0055 -0.2719NM_015480 PVRL3 0.2151 0.003548 -0.001848 0.006279 -0.00215 0.02263 0.01435 -1.101NM_024765 FLJ12401 -0.07577 0.263 0.001773 0.006341 -0.000399 0.6501 0.003348 -0.6887NM_003406 YWHAZ 0.02908 0.6275 -0.001579 0.00636 0.000373 0.6333 0.003209 -0.03604NM_014720 SLK 0.0798 0.2033 -0.001631 0.006599 -0.000587 0.4706 0.01804 -0.1718Contig60157_RC KIAA1244 0.2045 0.005154 -0.001824 0.006683 -0.002822 0.003274 0.005245 0.3063Contig36385_RC -0.2786 0.003227 0.002344 0.006774 0.000292 0.803 0.01134 -0.2356Contig55727_RC SLC7A11 0.1874 0.09037 -0.002842 0.006819 0.000434 0.7602 0.004828 -0.7453NM_032018 DKFZP547N043 0.1256 0.07224 -0.001787 0.006932 -0.000374 0.676 0.01423 -0.4993NM_153023 SPATA13 -0.2203 0.0009433 0.001592 0.007928 0.000105 0.8974 0.01153 -0.7152NM_020412 CHMP1.5 0.05855 0.4122 -0.001808 0.008344 2.72E-05 0.9767 0.009705 -0.5215NM_002483 CEACAM6 0.1659 0.2129 -0.003348 0.00846 0.000343 0.8423 0.007502 -1.014NM_022130 GOLPH3 0.05149 0.4093 -0.001577 0.00846 -0.000393 0.6287 0.01895 0.3994AK023565 AK023565 -0.1037 0.2297 0.00217 0.008525 -0.000288 0.7966 0.006843 -0.3864Contig47150_RC 0.2756 0.0004148 -0.001813 0.009068 -0.002519 0.01029 0.01233 -0.9444NM_005257 GATA6 0.2316 0.004881 -0.001952 0.009591 -0.002561 0.01572 0.016 -0.1132NM_006504 PTPRE 0.09811 0.2552 -0.002126 0.009787 0.000237 0.8323 0.008712 -0.5061NM_031453 C10orf45 0.1258 0.09132 -0.001817 0.009852 -0.00029 0.7618 0.01837 0.6647NM_000764 CYP2A7 -0.4962 0.008856 0.004503 0.009966 0.00052 0.8265 0.0168 -0.8172AF263613 IPLA2(GAMMA) 0.1359 0.05377 -0.001705 0.01007 -0.000279 0.7567 0.01898 -0.1374NM_145690 YWHAZ 0.01935 0.7409 -0.001447 0.01008 0.000446 0.5596 0.004519 0.1171NM_014670 BZW1 0.06415 0.283 -0.001465 0.01024 -0.000246 0.7506 0.0195 0.155NM_173658 FLJ36870 -0.2011 0.005609 0.001707 0.01031 -0.000391 0.6658 0.006252 -1.041NM_152523 FLJ40432 0.08769 0.1801 -0.001586 0.01077 -0.000154 0.8559 0.01748 -0.189NM_014399 TM4SF13 0.2319 0.008875 -0.002081 0.01078 -0.0033 0.004645 0.008342 0.3277AF116682 C6orf115 -0.3 7.31E-06 0.001439 0.01088 0.00255 0.001787 0.004328 0.6002ENST00000295733 ENST00000295733 -0.2321 0.02567 0.002467 0.01092 -0.000215 0.8703 0.01073 -1.026AK000776 0.254 0.006695 -0.002185 0.01101 -0.00322 0.008306 0.01214 -0.9592

NM_006393 NEBL 0.2629 0.002874 -0.002022 0.01164 -0.003544 0.002168 0.005116 -0.1853NM_181720 LOC257106 -0.2904 0.0001159 0.00166 0.0117 0.002416 0.009651 0.01373 0.5974Contig47483_RC 0.2755 9.07E-05 -0.001541 0.01193 -0.002864 0.001288 0.003518 -0.8621AK026286 AK026286 -0.08926 0.2255 0.001752 0.01239 -0.000184 0.8472 0.01186 0.0562NM_002658 PLAU 0.09439 0.3091 -0.002212 0.01243 0.00027 0.8226 0.01129 -1.104NM_021105 PLSCR1 0.0741 0.2817 -0.001638 0.0125 -8.52E-05 0.924 0.01844 -0.288NM_004196 CDKL1 -0.1664 0.005971 0.001376 0.01294 -0.000157 0.8347 0.01215 -1.181NM_006963 ZNF22 -0.1581 0.02004 0.001565 0.01303 -0.000232 0.7873 0.01105 0.1235NM_000762 CYP2A6 -0.4935 0.01065 0.004408 0.01322 0.000238 0.9216 0.01973 -0.7885Contig12755_RC HIPK2 -0.11 0.1086 0.001603 0.01325 5.19E-05 0.9531 0.01874 -0.9338NM_000323 RET 0.1698 0.1096 -0.002465 0.01384 0.00024 0.8602 0.01386 -1.202Contig54961_RC KPI2 0.2218 0.0002799 -0.001324 0.01386 -0.002081 0.0067 0.01197 -0.5107Contig51328_RC DKFZp434H2226 0.0327 0.6105 -0.001511 0.01392 8.14E-05 0.9225 0.0158 -0.3792NM_032251 FLJ37970 -0.2977 0.0002627 0.001758 0.0144 0.002757 0.007126 0.01269 0.3167AB011171 KIAA0599 -0.07448 0.3516 0.001862 0.01453 -0.000299 0.7736 0.0122 0.2571Contig23803_RC MLLT4 0.2793 0.0001923 -0.001601 0.01479 -0.002888 0.002346 0.005961 -1.198Contig57290_RC 0.2528 0.0001901 -0.001445 0.01496 -0.002569 0.00272 0.006671 -1.23NM_004760 STK17A 0.1931 0.005883 -0.001553 0.01527 -0.002233 0.01377 0.01958 -0.6676NM_130435 PTPRE 0.07261 0.4274 -0.002116 0.01529 0.000762 0.5227 0.0067 -0.2598NM_018134 FLJ10547 -0.002399 0.9709 0.001523 0.01543 -0.000361 0.6741 0.01035 -0.3826Contig57124_RC 0.3233 0.0007161 -0.002053 0.01584 -0.004202 0.000752 0.002525 -0.8079NM_001346 DGKG -0.04713 0.4981 0.001599 0.01604 -0.000155 0.8643 0.0166 -0.5204Contig23475_RC FLJ23471 0.1164 0.3503 -0.00285 0.01629 0.00065 0.6885 0.01145 -0.9696ENST00000258318 ENST00000258318 0.04197 0.4776 -0.00134 0.01737 0.0006 0.4369 0.005861 -0.9833NM_024590 FLJ23548 0.2745 0.0007507 -0.00172 0.01763 -0.003098 0.003062 0.007757 -0.8323NM_004161 RAB1A 0.01908 0.8212 -0.00191 0.01792 0.000277 0.8019 0.0166 0.3389NM_138763 BAX -0.3178 2.34E-05 0.001517 0.01792 0.002653 0.003999 0.009468 -0.2694NM_002659 PLAUR 0.0586 0.5664 -0.002303 0.01814 0.00093 0.4864 0.007345 -0.6472NM_032597 NYD-SP21 -0.5354 0.0008354 0.003372 0.01816 0.007658 0.000301 0.001208 -0.3586AK025209 COQ6 0.128 0.005181 -0.000982 0.01845 0.000181 0.7512 0.01537 -0.2316NM_002039 GAB1 0.2356 0.0009376 -0.001485 0.01932 -0.002592 0.00453 0.01067 -1.339NM_174938 MGC20553 0.2186 0.00616 -0.001699 0.01947 -0.003105 0.00311 0.008149 0.05156NM_003357 SCGB1A1 -0.3432 0.09396 0.004494 0.01947 -0.002046 0.4383 0.00678 1.286

SPREAD SHEET B. GENES WHOSE EXPRESSION WAS RELATED TO %

PARENCHYMA. ORDERED IN DESCENDING POSITIVE AND

ASCENDING NEGATIVE R VALUES Sequence Code Gene Name r

NM_181428 TMSL6 0.7312AJ303366 CHR3SYT 0.6276AK055696 AK055696 0.6275NM_020909 EPB41L5 0.626NM_000121 EPOR 0.6109NM_032495 HOP 0.6101NM_004041 ARRB1 0.6089Contig14761_RC KIAA1202 0.6075NM_002837 PTPRB 0.6028NM_016580 PCDH12 0.6012AK022093 AK022093 0.6008NM_015949 C7orf20 0.5996NM_020529 NFKBIA 0.5972NM_173160 FXYD4 0.5972NM_031413 CECR2 0.5951NM_018035 FLJ10241 0.5946NM_016533 NINJ2 0.5887AB020677 KIAA0870 0.5865NM_006701 DIM1 0.5858AF131834 0.5854NM_000092 COL4A4 0.5814NM_182643 DLC1 0.5801NM_022748 TENS1 0.5769NM_024565 FLJ14166 0.5747NM_006030 CACNA2D2 0.5733NM_001932 MPP3 0.5729NM_017898 FLJ20605 0.5726Contig50206_RC KIAA0977 0.5722NM_007039 PTPN21 0.5697NM_000176 NR3C1 0.5696NM_000136 FANCC 0.5694NM_032770 MGC16291 0.5687NM_001988 EVPL 0.5678NM_006319 CDIPT 0.5676NM_015234 GPR116 0.5666Contig58239_RC RBMS2 0.5655NM_014059 RGC32 0.5648NM_001955 EDN1 0.56NM_030965 SIAT7E 0.559Contig56276_RC 0.5586AK024556 AK024556 0.5584NM_004669 CLIC3 0.5578NM_021106 RGS3 0.5575

2

5

NM_016620 KIAA1221 0.5562NM_013279 C11orf9 0.5532NM_003598 TEAD2 0.5532NM_014247 PDZGEF1 0.5524NM_005904 MADH7 0.5513NM_001424 EMP2 0.551AB029025 KIAA1102 0.5482Contig54860_RC HIP1 0.5472Contig28034_RC 0.5469NM_018308 FLJ11042 0.5467Contig53226_RC DKFZp762C111 0.5466NM_002444 MSN 0.5464ENST00000284082 COL10A1 0.5464AL049397 PNAS-4 0.5449AF086016 NRP1 0.5443AF074993 CAV1 0.5442Contig48928_RC FLJ00138 0.5439NM_020455 GPR126 0.5436Contig15529_RC 0.5435Contig27464_RC SPIN 0.5433NM_006293 TYRO3 0.5427Contig28522_RC FLJ11127 0.5419NM_032800 FLJ14525 0.5407NM_001753 CAV1 0.5405NM_004557 NOTCH4 0.5401Contig21514_RC 0.5398Contig47005_RC 0.5395NM_003242 TGFBR2 0.5395NM_001709 BDNF 0.5393NM_024760 FLJ14009 0.5366NM_153612 HS3ST5 0.5363Contig21185_RC OCR2 0.5358NM_002933 RNASE1 0.5354NM_022304 HRH2 0.535NM_014100 PRO1770 0.5346NM_018211 KIAA1579 0.5332NM_006901 MYO9A 0.5323NM_030804 DKFZP434E213 0.5315NM_000827 GRIA1 0.5311NM_016929 CLIC5 0.5305ENST00000300760 ENST0000030076 0.5298NM_003468 FZD5 0.5294AB037722 NEDL2 0.5293AL162032 GPR133 0.5293NM_004337 C8orf1 0.5288NM_080920 GGTLA4 0.5282NM_016297 PCYOX1 0.528NM_016519 AMBN 0.5269NM_013324 CISH 0.5266NM_080865 GPR62 0.5262Contig28285_RC 0.5261

NM_052934 SLC26A9 0.526Contig37871_RC HKE2 0.5255NM_019086 FLJ20674 0.5245NM_001946 DUSP6 0.5244Contig44829_RC RGL3 0.5241NM_176792 MRPL43 0.5237NM_005388 PDCL 0.5236NM_030964 SPRY4 0.5228NM_152717 MGC35295 0.5227NM_017753 FLJ20300 0.5226Contig50520_RC 0.5205Contig39226_RC 0.5202NM_182908 DHRS2 0.52NM_018440 PAG 0.5194NM_005267 GJA8 0.5193NM_003979 RAI3 0.5191AK023803 ARF1 0.5187Contig10670_RC 0.5185Contig51369_RC LAF4 0.518Contig45859_RC CAV2 0.518NM_138375 LOC91768 0.5178AF131764 ATP6V1D 0.5176NM_000052 ATP7A 0.5174D86982 ANKS1 0.517ENST00000278232 ENST0000027823 0.5169NM_002845 PTPRM 0.5165Contig39389_RC 0.5164AF086461 DKFZP434L0718 0.5164NM_033159 HYAL1 0.5154NM_019074 DLL4 0.5152NM_147150 AKAP2 0.5151NM_153675 FOXA2 0.5151AK055564 RAI3 0.5145NM_021634 LGR7 0.5144Contig38603_RC 0.5143NM_003620 PPM1D 0.5139NM_178136 PDIP46 0.5136ENST00000296566 ENST0000029656 0.5131NM_000231 SGCG 0.5127Contig49512_RC FLJ20674 0.5125NM_177424 STX12 0.5125NM_178565 MGC35555 0.512ENST00000288059 ENST0000028805 0.5119NM_134421 HPCAL1 0.5112NM_014461 CNTN6 0.5106NM_004657 SDPR 0.5102NM_019601 BK65A6.2 0.5102NM_002898 RBMS2 0.5101Contig50276_RC MGC15887 0.5089NM_015691 KIAA1280 0.5087NM_032866 FLJ14957 0.5084

Contig37538_RC 0.5079NM_025252 ALS2CR9 0.5078AF052094 EPAS1 0.5075NM_017706 FLJ20195 0.5074NM_003128 SPTBN1 0.5066NM_024738 FLJ21415 0.5065NM_004636 SEMA3B 0.5063NM_016529 ATP8A2 0.5063NM_007312 HYAL1 0.5055NM_006059 LAMC3 0.5053NM_002149 HPCAL1 0.505AL050154 0.5048NM_000091 COL4A3 0.5046NM_181846 HKR2 0.5046NM_000815 GABRD 0.5046NM_170734 BDNF 0.5046NM_015993 TM4SF11 0.504Contig34811_RC ARRB1 0.5039NM_006243 PPP2R5A 0.5026NM_152457 FLJ33071 0.5025AL359053 ITGB6 0.5021Contig39087_RC ZDHHC3 0.502AF174600 LMO7 0.5019NM_001200 BMP2 0.5018NM_016647 LOC51337 0.5015NM_138331 RNASE8 0.5014NM_018971 GPR27 0.5009NM_006092 CARD4 0.5007NM_017582 UBE2Q 0.5001NM_020130 C8orf4 0.5NM_138445 GPR146 0.4998NM_004469 FIGF 0.4996Contig63683_RC EPB41L5 0.4996NM_181600 KRTAP13-4 0.4996NM_182793 TMSL2 0.4995AF116637 CaMKIINalpha 0.4988NM_014725 STARD8 0.4982NM_024743 FLJ21934 0.4979Contig36832_RC 0.4977NM_031479 INHBE 0.4976NM_000038 APC 0.4965AL117657 ZNF197 0.4962NM_012237 SIRT2 0.496NM_032233 FLJ23027 0.4956NM_052855 MGC15396 0.4955Contig40418_RC IHH 0.4954Contig1389_RC MAPK9 0.4951NM_002516 NOVA2 0.4951ENST00000287674 ENST000002876 0.4949NM_173540 FUT11 0.4949NM_152748 FLJ31340 0.4948

NM_005308 GPRK5 0.4943NM_002246 KCNK3 0.4939Contig39531_RC FLJ30681 0.4939Contig49480_RC MGC3040 0.4937NM_018192 MLAT4 0.4935NM_018584 CaMKIINalpha 0.4934Contig28079_RC 0.4932NM_001430 EPAS1 0.4931Contig38500_RC SSTR1 0.493AK056954 HSD17B7P1 0.493AJ012492 ETV1 0.4929NM_000908 NPR3 0.4925NM_031845 MAP2 0.4925NM_005794 DHRS2 0.4923NM_032181 FLJ13391 0.4919NM_019855 CABP5 0.4919Contig35927_RC 0.4918NM_131915 LOC150236 0.4912NM_005402 RALA 0.4911NM_002020 FLT4 0.491NM_006005 WFS1 0.4902NM_006331 C2F 0.4899NM_003376 VEGF 0.4897NM_022062 PKNOX2 0.4894M30474 GGT2 0.4892NM_007324 MADHIP 0.4892NM_152611 C20orf75 0.4892ENST00000277309 ENST0000027730 0.4889NM_175733 LOC143425 0.4887NM_024419 PGS1 0.4887NM_032890 DISP1 0.4884NM_015313 ARHGEF12 0.488AK026099 C20orf155 0.4878NM_005323 HIST1H1T 0.4874Contig46668_RC PAG 0.4873NM_002252 KCNS3 0.4871Contig25670_RC 0.4868NM_152900 MAGI-3 0.4863NM_024033 MGC5242 0.4863NM_182499 DKFZp434M202 0.4862NM_032755 MGC15634 0.486NM_001052 SSTR4 0.4853NM_013384 LASS2 0.4851NM_002966 S100A10 0.4849NM_000513 OPN1MW 0.4849AB046859 KIAA1639 0.4848NM_022101 FLJ22965 0.4848NM_014547 TMOD3 0.4847NM_013399 C16orf5 0.4844NM_012294 GFR 0.4843NM_006588 SULT1C2 0.4843

0

NM_001945 DTR 0.4834NM_013421 GGT1 0.4832NM_001795 CDH5 0.483NM_019084 FLJ10895 0.4828NM_003317 TITF1 0.4827NM_017790 RGS3 0.4827NM_014656 KIAA0040 0.4823Contig40145_RC QKI 0.4821NM_007221 PMF1 0.4821Contig27827_RC 0.4818Contig26434_RC 0.4818Contig43182_RC TITF1 0.4815NM_022833 DKFZP434H082 0.4814AL080215 0.4812NM_144639 FLJ31300 0.4812AL512695 MGC20785 0.4808NM_002310 LIFR 0.4807NM_016133 INSIG2 0.4807AK000001 C9orf28 0.4805Contig28085_RC BRD4 0.4801NM_003725 RODH 0.4801AF142421 QKI 0.4798Contig55749_RC 0.4798Contig42141_RC DCOHM 0.4797NM_015141 KIAA0089 0.4797NM_002608 PDGFB 0.4793AK000799 CCNK 0.4792NM_000024 ADRB2 0.4792NM_022351 EFCBP1 0.4791NM_019018 FLJ11127 0.479NM_014721 C6orf56 0.4789AF069135 FLJ32783 0.4788NM_032402 PCDHGC3 0.4788NM_033397 KIAA1754 0.4787Contig75_RC RPS6KA2 0.4785NM_002744 PRKCZ 0.4784NM_006505 PVR 0.4784NM_080625 C20orf160 0.4783Contig39710_RC 0.4778NM_032609 COX4I2 0.4777NM_012334 MYO10 0.4777Contig33759_RC 0.4776NM_014333 IGSF4 0.4772NM_018458 KIAA1280 0.4769NM_003180 SYT5 0.4769AB033027 KIAA1201 0.4766NM_006675 NET-5 0.4765NM_021135 RPS6KA2 0.4763NM_014580 SLC2A8 0.4762AL117611 DKFZP434D193 0.4756NM_022154 SLC39A8 0.4756

NM_152543 FLJ25371 0.4755NM_004392 DACH 0.4754NM_024847 TMC7 0.4753NM_016511 CLEC1 0.4751NM_025214 SE57-1 0.475AL157484 0.4748NM_173496 FLJ32798 0.4744NM_000144 FRDA 0.4744NM_014572 LATS2 0.4743NM_000060 BTD 0.4742NM_152310 ELOVL3 0.4737AF088500 TCRBV19S1P 0.4735NM_021647 KIAA0626 0.4735Contig42867 LRRFIP1 0.4734AB067499 KIAA1912 0.4733Contig34983_RC 0.4732NM_007023 CGEF2 0.4731NM_173673 FLJ34503 0.4731Contig47922_RC ANKRD13 0.4728NM_080839 GGTL4 0.4728Contig28807_RC 0.4724NM_021210 TRAPPC1 0.4724AB002374 KIAA0376 0.4721NM_152830 ACE 0.4717AB033100 KIAA1274 0.4715D86971 KIAA0217 0.4714Contig45090_RC 0.4713NM_002025 FMR2 0.4711Contig44088_RC 0.4709NM_001195 BFSP1 0.4709NM_024778 RNF127 0.4706NM_022109 CTL1 0.4704NM_014178 STXBP6 0.4703NM_080672 SCAPIN1 0.4699NM_032255 DKFZp434I1930 0.4696NM_001136 AGER 0.4696NM_003597 TIEG2 0.4695NM_003683 D21S2056E 0.4695NM_024767 DLC1 0.4692NM_006122 MAN2A2 0.4691NM_178313 SPTBN1 0.4691NM_032867 FLJ14966 0.4688AF064843 0.4686Contig48249_RC FLJ10849 0.4685NM_024693 ECHDC3 0.4684NM_014330 PPP1R15A 0.4683NM_005768 C3F 0.468NM_000542 SFTPB 0.4674NM_013241 FHOD1 0.4673NM_033281 MRPS36 0.4673ENST00000295535 DKFZp761I1011 0.4673

NM_033119 NKD1 0.4671NM_015316 PPP1R13B 0.467NM_019071 ING3 0.4669NM_005619 RTN2 0.4669NM_020546 ADCY2 0.4669NM_000459 TEK 0.4668NM_014603 HUMPPA 0.4668NM_172318 KCNG1 0.4668NM_003189 TAL1 0.4666NM_052946 NOSTRIN 0.4666NM_002151 HPN 0.4665NM_173642 MGC47816 0.466NM_001204 BMPR2 0.4659NM_002849 PTPRR 0.4659NM_003460 ZP2 0.4658Contig26584_RC KCNK3 0.4655NM_005996 TBX3 0.4655NM_181604 KRTAP6-2 0.4652NM_032897 MGC14436 0.4652NM_006575 MAP4K5 0.4649AL137508 ARHGEF10 0.4647NM_032196 KIAA1259 0.4644AL049990 0.4643NM_005795 CALCRL 0.4643NM_016325 ZNF274 0.4642NM_003280 TNNC1 0.4637AK056723 LOC158376 0.4637AF038171 0.4631Contig21797_RC NRP1 0.463Contig28941_RC ATP6V0E 0.4626NM_004938 DAPK1 0.4626NM_020248 CTNNBIP1 0.4624NM_052889 COP 0.4624NM_170736 KCNJ15 0.4624NM_000020 ACVRL1 0.4623AL133641 TOM1L2 0.4622NM_144777 SCEL 0.4622Contig47453_RC FLJ36748 0.4621NM_032111 MRPL14 0.4619NM_005550 KIFC3 0.4618Contig40026_RC 0.4617NM_016532 SKIP 0.4615Contig16208_RC EDNRA 0.4614Contig26835_RC 0.4611NM_018064 C6orf166 0.4611NM_003305 TRPC3 0.461NM_005424 TIE 0.461NM_016561 BFAR 0.461NM_017750 FLJ20296 0.4609NM_000717 CA4 0.4609Contig50979_RC ADRB1 0.4609

NM_145307 RTKN2 0.4609NM_004625 WNT7A 0.4608NM_014421 DKK2 0.4607NM_001058 TACR1 0.4607NM_152721 MGC20785 0.4607NM_005139 ANXA3 0.4606NM_022739 SMURF2 0.4606Contig50921_RC 0.4605NM_002538 OCLN 0.4603U79257 FBXO21 0.4602NM_002072 GNAQ 0.4598AL080103 C22orf1 0.4596Contig659_RC PPIG 0.4592Contig50156_RC 0.4592NM_079836 TUBA2 0.4592NM_007227 GPR45 0.459NM_138961 ESAM 0.4589NM_054022 GOSR2 0.4589AL122049 C12orf2 0.4588NM_004816 X123 0.4588NM_138492 MGC21644 0.4582NM_173686 NM_173686 0.4579Contig27451_RC ETV1 0.4577NM_181703 GJA5 0.4576AK025126 AK025126 0.4575Contig41035 GOLPH4 0.4574NM_152690 DPM2 0.4574NM_004799 MADHIP 0.4573NM_001543 NDST1 0.4573NM_006668 CYP46A1 0.457AF088007 AF088007 0.457NM_147202 C9orf25 0.457NM_002204 ITGA3 0.4569NM_000888 ITGB6 0.4567NM_014505 KCNMB4 0.4566NM_014758 SNX19 0.4564NM_005338 HIP1 0.4564AF387612 gm117 0.4557NM_133282 RAD1 0.4557NM_001386 DPYSL2 0.4556NM_001650 AQP4 0.4554NM_052851 STARD13 0.4553NM_003709 KLF7 0.4553NM_002737 PRKCA 0.4552AL049449 GAB1 0.4549NM_004841 RASAL2 0.4549NM_006549 CAMKK2 0.4547Contig53165_RC FLJ42654 0.4544NM_021092 PPY2 0.4543NM_007118 TRIO 0.4542AF086503 PTTG1IP 0.4541

5

NM_006287 TFPI 0.454NM_001233 CAV2 0.4539NM_000899 KITLG 0.4539NM_001707 BCL7B 0.4539NM_004574 PNUTL2 0.4538NM_006377 UNC13 0.4537NM_054032 MRGX4 0.4536NM_152536 FLJ39957 0.4536AL117595 GIT1 0.4535NM_003919 SGCE 0.4535Contig45185_RC GAB1 0.4534NM_031859 PCDHA10 0.4533NM_017919 STX17 0.4533AF131806 0.4532NM_138969 RDH-E2 0.4532NM_015946 PELO 0.4531NM_007322 RANBP3 0.4531NM_003391 WNT2 0.4529NM_016586 MBIP 0.4528NM_003165 STXBP1 0.4526NM_003159 CDKL5 0.4525NM_014588 VSX1 0.4525NM_016281 JIK 0.4522Contig39234 0.4521NM_003193 TBCE 0.452NM_015617 PYGO1 0.4517NM_181534 KRT25A 0.4515NM_006077 CBARA1 0.4515NM_000015 NAT2 0.4513NM_013940 OR10H1 0.4512Contig56096 IL17D 0.451AB033076 KIDINS220 0.4508NM_014646 LPIN2 0.4507NM_032219 FLJ22269 0.4505NM_002089 CXCL2 0.4504NM_015193 ARC 0.4504NM_024934 FLJ22659 0.4503Contig21464_RC 0.4502Contig19205_RC S100A10 0.4502AF086064 SLC8A3 0.4502ENST00000279413 ENST000002794 0.45NM_145864 KLK3 0.45NM_032435 KIAA1804 0.4498NM_020974 SCUBE2 0.4497Contig58475_RC PSMB2 0.4495AL133047 DKFZp434D021 0.4495NM_021819 LMAN1L 0.4494NM_033302 ADRA1A 0.4494NM_000440 PDE6A 0.4491NM_003877 SOCS2 0.449NM_144623 FLJ32784 0.4485

NM_020456 C13orf1 0.4485NM_032291 DKFZp761D221 0.4483NM_001604 PAX6 0.4483NM_012072 C1QR1 0.4483NM_000873 ICAM2 0.4481NM_144962 MGC22776 0.4476ENST00000299487 ENST0000029948 0.4473Contig47994_RC RAP1A 0.4472NM_002394 SLC3A2 0.4472NM_025074 FRAS1 0.447NM_152727 CPNE2 0.447NM_005512 GARP 0.4469Contig55886_RC 0.4468NM_013245 VPS4A 0.4468NM_003693 SCARF1 0.4467NM_025076 UXS1 0.4467NM_178520 FLJ38792 0.4466NM_020753 CASKIN2 0.4465NM_004970 IGFALS 0.4464NM_080759 DACH 0.4462Contig331_RC LOC90799 0.4461NM_017705 MPRG 0.446NM_014767 SPOCK2 0.446ENST00000300034 ENST0000030003 0.4457NM_033375 MYO1C 0.4457Contig46598_RC MJD 0.4456NM_080608 C20orf165 0.4455NM_133377 RAD1 0.4453NM_182536 GDDR 0.4452NM_001112 ADARB1 0.4451NM_173075 APBB2 0.4449NM_015277 NEDD4L 0.4448NM_003238 TGFB2 0.4447AK054795 MGC15396 0.4445AK021881 HIF3A 0.4445AK000297 KIAA0601 0.4444NM_032098 PCDHGB4 0.4442NM_024894 FLJ14075 0.4442NM_002282 KRTHB3 0.4438NM_000520 HEXA 0.4435AK026728 AQP4 0.4433NM_175060 C14orf27 0.4431NM_017595 KBRAS2 0.4427NM_019559 F11 0.4426AL049313 0.4425NM_139032 MAPK7 0.4424NM_002739 PRKCG 0.4423NM_130469 JDP2 0.4422NM_000067 CA2 0.4419NM_016839 RBMS1 0.4416NM_001234 CAV3 0.4416

NM_181486 TBX5 0.4416ENST00000299100 ENST0000029910 0.4414AK022044 AK022044 0.4413NM_016438 CLST11240 0.4411NM_020142 LOC56901 0.441Contig47262_RC 0.4408AB002438 SEMA6A 0.4408NM_053284 WFIKKN 0.4408NM_017584 ALDRL6 0.4407Contig35088_RC 0.4406NM_152421 MGC20262 0.4405NM_004160 PYY 0.4404NM_005205 COX6A2 0.4402Contig54727_RC FGD5 0.4399NM_001171 ABCC6 0.4397NM_145345 SOC 0.4397NM_017893 SEMA4G 0.4393Contig43639_RC UCHL1 0.439AL359062 COL8A1 0.4389NM_014745 KIAA0233 0.4389NM_016369 CLDN18 0.4387NM_002386 MC1R 0.4385Contig52770_RC IMP-1 0.4385NM_005551 KLK2 0.4385NM_004177 STX3A 0.4381NM_018910 PCDHA7 0.4381NM_024032 MGC3130 0.4376NM_001649 APXL 0.4376NM_007288 MME 0.4375NM_024016 HOXB8 0.4374NM_018058 CRTAC1 0.4373NM_032903 MGC14425 0.4373AL157495 SMARCA5 0.4373AB040883 KIAA1450 0.4372ENST00000237296 KIAA0408 0.4372NM_001290 LDB2 0.4371NM_015959 TMX2 0.4371NM_000305 PON2 0.437NM_032053 PCDHGA4 0.437NM_001752 CAT 0.4367ENST00000293220 ENST0000029322 0.4366NM_153264 FLJ35880 0.4363NM_024068 MGC2731 0.4363NM_001331 CTNND1 0.4359NM_021232 PRODH2 0.4359NM_000525 KCNJ11 0.4359NM_182918 ERG 0.4359NM_006281 STK3 0.4358NM_032311 PDIP46 0.4358NM_020828 ZFP28 0.4358NM_000442 PECAM1 0.4355

NM_003827 NAPA 0.4354NM_000902 MME 0.4352NM_003996 GPX5 0.4352NM_003045 SLC7A1 0.4352NM_002253 KDR 0.4351NM_005656 TMPRSS2 0.4349AK054768 LOC56181 0.4348NM_153365 FLJ90013 0.4348Contig20685_RC 0.4346NM_017748 FLJ20291 0.4345NM_003843 SCEL 0.4344NM_006375 COVA1 0.4344Contig20441_RC NAP5 0.4343NM_005854 RAMP2 0.4343Contig43791_RC TGFB2 0.4342NM_013281 FLRT3 0.4341NM_024574 FLJ23191 0.434NM_014214 IMPA2 0.4339NM_024709 FLJ14146 0.4339AF131852 Unknown 0.4337Contig52957_RC 0.4336AK021730 AK021730 0.4336NM_133265 AMOT 0.4336NM_000801 FKBP1A 0.4336NM_012338 TM4SF12 0.4335NM_018110 DOK4 0.4335NM_020796 SEMA6A 0.4334ENST00000270547 ENST0000027054 0.4333NM_005020 PDE1C 0.433NM_007021 C10orf10 0.433AL137414 AL137414 0.4329NM_000725 CACNB3 0.4328NM_018901 PCDHA10 0.4328BC001437 FLJ20674 0.4328ENST00000298672 ENST000002986 0.4326NM_001528 HGFAC 0.4322AB037810 SIPA1L2 0.4321Contig57398_RC KIAA1450 0.4316NM_182524 LOC152687 0.4316Contig58491_RC TRIP11 0.4315NM_000821 GGCX 0.4313NM_031231 APBA2BP 0.4313NM_002374 MAP2 0.4312NM_024563 FLJ14054 0.4312Contig12419_RC 0.4311NM_024534 FLJ12684 0.431NM_021959 PPP1R11 0.4308NM_014859 KIAA0672 0.4307NM_018243 FLJ10849 0.4307Contig48769_RC LOC131873 0.4306NM_012090 MACF1 0.4305

NM_002381 MATN3 0.4305NM_002692 POLE2 0.4304AL117662 ZDHHC5 0.4303NM_016652 CRNKL1 0.4302NM_001146 ANGPT1 0.43AB075832 KIAA1952 0.43NM_021622 PLEKHA1 0.4299NM_024768 FLJ12057 0.4299NM_025051 FLJ23022 0.4298Contig38962_RC CUL1 0.4295NM_006312 NCOR2 0.4294AF085871 CLDN11 0.4294NM_000754 COMT 0.4292Contig36104_RC GOLGA1 0.4292NM_000684 ADRB1 0.4292NM_001742 CALCR 0.429NM_000148 FUT1 0.429NM_003940 USP13 0.4289Contig21843_RC 0.4289X89666 OR2M4 0.4289NM_007310 COMT 0.4288NM_006044 HDAC6 0.4286NM_004234 ZNF235 0.4286NM_007121 NR1H2 0.4284NM_018381 FLJ11286 0.428NM_004976 KCNC1 0.428NM_031313 ALPPL2 0.4278AL137356 NRXN2 0.4278AF088326 IGHG3 0.4276AK023877 FLJ13815 0.4275NM_022307 ICA1 0.4275NM_003206 TCF21 0.4274AB053318 ALS2CR17 0.4274NM_007203 AKAP2 0.4272NM_058184 C21orf42 0.4272Contig42328_RC EG1 0.4271NM_024600 FLJ20898 0.427NM_006718 PLAGL1 0.4269NM_000394 CRYAA 0.4265NM_017624 FLJ20019 0.4265Contig27487_RC 0.4264NM_004028 AQP4 0.4264AB007974 0.4264NM_014826 CDC42BPA 0.4264NM_007289 MME 0.4263NM_005434 BENE 0.4263NM_032845 FLJ14816 0.4262NM_005992 TBX1 0.4262Contig29067_RC 0.4261NM_080878 ITLN2 0.4261NM_002376 MARK3 0.4261

NM_016653 ZAK 0.426NM_004851 NAP1 0.4258NM_021245 MYOZ1 0.4256Contig1682_RC RCP 0.4256Contig21177_RC 0.4254NM_004420 DUSP8 0.4253NM_174936 PCSK9 0.4253NM_019108 FLJ12886 0.4251NM_052867 VGCNL1 0.425NM_032562 PLA2G13 0.4248NM_181552 CUTL1 0.4247NM_033027 AXUD1 0.4246NM_002821 PTK7 0.4244Contig54395_RC CLDN18 0.4244AK024653 AK024653 0.4243NM_177995 PTP9Q22 0.4243NM_000458 TCF2 0.4242NM_003019 SFTPD 0.4242NM_031467 SLC4A9 0.4242NM_000831 GRIK3 0.4241NM_005988 SPRR2A 0.4239AL133031 HCAP-G 0.4239NM_005983 SKP2 0.4238NM_001452 FOXF2 0.4235X99687 MECP2 0.4235NM_030923 DKFZP566N034 0.4235NM_000565 IL6R 0.4233NM_130468 D4ST-1 0.4232NM_152547 FLJ32535 0.4232AK057980 AK057980 0.423NM_080605 B3GALT6 0.4229NM_002428 MMP15 0.4226NM_015868 KIR2DL3 0.4225NM_031232 APBA2BP 0.4224NM_079422 MYL1 0.4223NM_016365 nebulette 0.4222NM_178493 LOC147111 0.4222NM_001965 EGR4 0.4221NM_001976 ENO3 0.4221NM_145272 LOC146853 0.4221NM_138288 C14orf147 0.4221ENST00000275760 MTPN 0.422AK000493 AK000493 0.4218NM_002442 MSI1 0.4217NM_020439 CAMK1G 0.4216Contig42884_RC EHD3 0.4215AB046806 KIAA1586 0.4215AB051470 KIAA1683 0.4214NM_153029 FLJ31821 0.4213ENST00000285156 FLJ34907 0.4211NM_015692 VIP 0.4211

Contig8597_RC 0.4207Contig38093_RC 0.4207NM_032016 STARD3NL 0.4205NM_001451 FOXF1 0.4204NM_178140 PDZK3 0.4203NM_014419 DKKL1-pending 0.4203NM_145207 SPATA5 0.4202NM_005454 CER1 0.42NM_173560 RFXDC1 0.42NM_022901 LRRC19 0.4199NM_145811 CACNG5 0.4199NM_007036 ESM1 0.4197Contig36931_RC 0.4197NM_138808 LOC132200 0.4197NM_022566 MESDC1 0.4196NM_024315 C7orf23 0.4194NM_022475 HHIP 0.4192NM_173646 FLJ39660 0.4191NM_002185 IL7R 0.4189NM_152299 384D8-2 0.4189NM_000855 GUCY1A2 0.4189NM_015914 LOC51061 0.4189NM_014232 VAMP2 0.4188NM_004696 SLC16A4 0.4187X89671 OR5E1P 0.4187AK023058 AK023058 0.4186NM_025151 RCP 0.4186NM_005840 SPRY3 0.4186NM_031941 MCC2 0.4184NM_003991 EDNRB 0.4182Contig42728_RC CRTAC1 0.4179NM_005354 JUND 0.4177NM_013293 TRA2A 0.4176NM_032045 KREMEN1 0.4176NM_002817 PSMD13 0.4176NM_005757 MBNL2 0.4176NM_000860 HPGD 0.4174NM_020451 SEPN1 0.4174NM_024551 ADIPOR2 0.4173NM_057093 CRYBA2 0.4173NM_020801 KIAA1376 0.4172Contig55990_RC ADPRH 0.4171NM_134264 WSB1 0.4171NM_004093 EFNB2 0.417AF098485 NAP1L 0.4168Contig23977_RC 0.4166Contig6615_RC 0.4165NM_014070 C6orf15 0.4165NM_021937 SELB 0.4164Contig33579_RC 0.4163Contig56229 EZI 0.4163

NM_004730 ETF1 0.4161NM_005012 ROR1 0.416AL117623 0.4158NM_000240 MAOA 0.4157NM_176783 PSME1 0.4153Contig16346_RC IGSF4 0.4152NM_001642 APLP2 0.4152AK027246 SC5DL 0.4152NM_024498 ZNF117 0.4152Contig43708_RC 0.4151NM_152631 FLJ35782 0.415NM_015068 PEG10 0.415NM_014811 KIAA0649 0.4149AL049260 AL049260 0.4148NM_015103 PLXND1 0.4147NM_031852 PCDHA7 0.4147X81005 HCG4P6 0.4146NM_002233 KCNA4 0.4145NM_000227 LAMA3 0.4145NM_004454 ETV5 0.4144NM_020127 TUFT1 0.4144NM_004664 LIN7A 0.4143NM_152321 FLJ32115 0.4143NM_024758 AGMAT 0.4143NM_012478 WBP2 0.4142NM_025170 FLJ12987 0.4142AB011123 KIAA0551 0.4141AL049450 LOC339287 0.4141NM_130847 AMOTL1 0.414NM_130783 LOC90139 0.414NM_000832 GRIN1 0.4138NM_012129 CLDN12 0.4138Contig52705_RC CREBBP 0.4137NM_000401 EXT2 0.4137NM_014489 FRAG1 0.4135NM_006814 PSMF1 0.4135NM_152588 DKFZp762A217 0.4135NM_005549 KCNA10 0.4135NM_000226 KRT9 0.4134AB032962 GPR158 0.4134NM_001727 BRS3 0.4134AB002339 N4BP3 0.4131AB037778 KIAA1357 0.4131NM_001300 COPEB 0.4131NM_176883 TAS2R41 0.413NM_021023 FHR-3 0.4129NM_014426 SNX5 0.4128NM_018469 HT008 0.4127NM_024949 BOMB 0.4126NM_004861 CST 0.4125NM_017791 C14orf58 0.4125

NM_000364 TNNT2 0.4124Contig48988_RC ABHD5 0.4123Contig25622_RC 0.4122NM_080491 GAB2 0.4122NM_006034 TP53I11 0.4121ENST00000272035 ENST0000027203 0.4119NM_018400 SCN3B 0.4119NM_053013 ENO3 0.4118NM_015178 RHOBTB2 0.4118NM_002465 MYBPC1 0.4117NM_014672 KIAA0391 0.4117NM_014474 ASML3B 0.4115NM_177524 MEST 0.4114NM_018978 CSE-C 0.4114Contig35822_RC LMCD1 0.4113NM_020205 ZA20D1 0.4113NM_017715 ZNF3 0.4113NM_006593 TBR1 0.4111Contig57124_RC 0.411NM_005060 RORC 0.411NM_016322 RAB14 0.4109NM_017542 POGK 0.4108NM_002716 PPP2R1B 0.4107NM_014275 MGAT4B 0.4106NM_014092 PRO1575 0.4106NM_023918 TAS2R8 0.4106AL109716 0.4104NM_006481 TCF2 0.4103NM_004529 MLLT3 0.4103NM_014181 HSPC159 0.4102NM_153823 GSG1 0.4102Contig19216_RC 0.4099NM_015564 LRRTM2 0.4098NM_031207 HT036 0.4098NM_001043 SLC6A2 0.4097Contig6452_RC 0.4097NM_021126 MPST 0.4097NM_002732 PRKACG 0.4096NM_020647 JPH1 0.4096NM_025217 ULBP2 0.4095NM_002291 LAMB1 0.4094NM_173633 FLJ90805 0.4094NM_002066 GML 0.4092AB032961 Spir-1 0.4089NM_005262 GFER 0.4089NM_003941 WASL 0.4087NM_004815 PARG1 0.4085NM_017723 FLJ20245 0.4085NM_030957 ADAMTS10 0.4085AF075087 AF075087 0.4084NM_003971 SPAG9 0.4084

NM_053279 C8orf13 0.4082NM_018969 SREB3 0.4081NM_178172 LOC338328 0.4081NM_016836 RBMS1 0.408NM_032515 BOK 0.4079NM_013938 OR10H3 0.4078NM_145661 APOL4 0.4078Contig46242_RC 0.4077Contig17322_RC 0.4076ENST00000298888 ENST0000029888 0.4076ENST00000276514 ENST000002765 0.4076AK026499 AK026499 0.4073Contig39152_RC 0.4072NM_001957 EDNRA 0.4072NM_013390 TMEM2 0.4072NM_003595 TPST2 0.4072NM_015328 KIAA0828 0.4072NM_032812 PLXDC2 0.4071NM_006192 PAX1 0.4071Contig52358_RC 0.407NM_003277 CLDN5 0.407Contig41195_RC 0.407AF052117 CLCN4 0.4069NM_000039 APOA1 0.4069NM_002090 CXCL3 0.4068Contig21353_RC PROK2 0.4067NM_032859 FLJ14906 0.4067NM_018948 MIG-6 0.4067NM_012164 FBXW2 0.4065AB046788 ROBO2 0.4064NM_144703 C20orf40 0.4064NM_178536 MGC48935 0.4064AL117627 0.4062AK055674 MAP2 0.4062NM_004286 GTPBP1 0.4061NM_020346 SLC17A6 0.406NM_007191 WIF1 0.406NM_006056 GPR66 0.4059U17626 U17626 0.4058NM_002141 HOXA4 0.4058NM_024740 DIBD1 0.4057NM_020323 ABCD4 0.4057AK001875 0.4056NM_002192 INHBA 0.4056NM_003893 LDB1 0.4056NM_015117 KIAA0150 0.4056Contig55540_RC PRKAG1 0.4055NM_022897 RANBP17 0.4055AL512721 AL512721 0.4053NM_024954 FLJ11807 0.4053NM_033044 MACF1 0.4053

AI127067_RC SLC29A2 0.4051NM_000102 CYP17A1 0.4051ENST00000296831 ENST0000029683 0.4051NM_002959 SORT1 0.4051X98834 SALL2 0.405NM_018567 TNS 0.405Contig28161_RC 0.405NM_020527 C10orf1 0.4048NM_000033 ABCD1 0.4048RSE_00000478328 RSE_0000047832 0.4047NM_000859 HMGCR 0.4047Contig36574 KPNA3 0.4045NM_006771 KRTHA8 0.4044NM_002785 PSG11 0.4044NM_006131 BMP1 0.4043NM_152662 QSCN6 0.4043NM_002641 PIGA 0.4042NM_018964 SLC37A1 0.4041Contig11027_RC 0.4041NM_022758 C6orf106 0.4041NM_021046 LOC57830 0.4041Contig51846_RC 0.4039NM_018488 TBX4 0.4039NM_002944 ROS1 0.4037NM_001875 CPS1 0.4037NM_014458 AB026190 0.4037NM_006424 SLC34A2 0.4036NM_001608 ACADL 0.4035NM_182926 KTN1 0.4034NM_012392 PEF 0.4033NM_005684 GPR52 0.4032AK001903 0.403NM_005358 LMO7 0.4028NM_002999 SDC4 0.4027AL050013 DAAM1 0.4027NM_181512 MRPL21 0.4027Contig51293_RC 0.4026NM_015156 RCOR 0.4026NM_153342 FLJ90024 0.4026NM_138799 LOC129642 0.4025NM_002011 FGFR4 0.4025NM_025243 SLC19A3 0.4025NM_003873 NRP1 0.4023D79998 KCTD2 0.4022NM_182623 FLJ36766 0.4022NM_015094 HIC2 0.4021NM_014836 RHOBTB1 0.4021NM_001879 MASP1 0.4021NM_005865 PRSS16 0.402NM_020134 DPYSL5 0.4019NM_004062 CDH16 0.4018

6

NM_152850 PIGO 0.4018NM_170719 FLJ12661 0.4017NM_182646 CPEB2 0.4017Contig48007_RC 0.4016NM_005942 MOCS1 0.4016AB037749 KIAA1328 0.4015Contig32558_RC USP13 0.4015NM_024591 FLJ11749 0.4015NM_017856 FLJ20514 0.4013Contig56261_RC GPR153 0.4012NM_000316 PTHR1 0.4011NM_017834 FLJ20464 0.4011NM_022068 FLJ23403 0.4009NM_130797 DPP6 0.4007Contig56262_RC 0.4006NM_001667 ARL2 0.4005NM_001115 ADCY8 0.4004NM_021247 PRM3 0.4004NM_002563 P2RY1 0.4003NM_002773 PRSS8 0.4001NM_015511 C20orf4 0.4001NM_013960 NRG1 0.4001NM_004827 ABCG2 0.3998NM_005206 CRK 0.3998NM_022834 FLJ22215 0.3998AL137535 0.3997NM_005474 HDAC5 0.3995NM_017509 KLK15 0.3994NM_006987 RPH3AL 0.3993NM_015623 DKFZP564D166 0.3992NM_002336 LRP6 0.3992NM_020699 P66beta 0.3992NM_152526 ALS2CR19 0.3988AF007149 0.3986Contig51252 0.3986NM_032134 DKFZP434P031 0.3986AK025924 AK025924 0.3985NM_182487 LOC169611 0.3985Contig53619_RC BCL6B 0.3983NM_025140 FLJ22471 0.3981NM_024505 NOX5 0.3981NM_024621 FLJ12604 0.3981ENST00000298494 NRG3 0.398ENST00000266507 ENST0000026650 0.398NM_080616 C20orf112 0.398NM_017931 FLJ20699 0.3979NM_152223 PPEF1 0.3979AB033092 MTA3 0.3978NM_000543 SMPD1 0.3978Contig27636_RC 0.3977NM_014696 KIAA0514 0.3977

4

NM_004795 KL 0.3976NM_015987 HEBP1 0.3974NM_032510 PARD6G 0.3972NM_152834 DKFZp434C171 0.3971NM_004317 ASNA1 0.3971NM_145034 IFRG15 0.3971NM_025195 C8FW 0.3971NM_014746 RNF144 0.397NM_175727 IL5RA 0.397Contig40464_RC 0.3969NM_022052 NXF3 0.3967NM_006806 BTG3 0.3965NM_000777 CYP3A5 0.3964NM_000778 CYP4A11 0.3964NM_004922 SEC24C 0.3964NM_019053 SEC15L1 0.3964NM_001097 ACR 0.3964NM_014600 EHD3 0.3963NM_014357 SPRL1B 0.3963NM_020426 LOC57151 0.3963NM_012420 IFIT5 0.3962NM_133629 RAD51L3 0.3961RSE_00000024784 RSE_0000002478 0.396NM_139161 CRB3 0.396NM_000115 EDNRB 0.3959NM_173854 SLC41A1 0.3959AK000535 ETS2 0.3958NM_004484 GPC3 0.3957AJ420555 IBRDC2 0.3956NM_032086 PCDHGA6 0.3956NM_014167 HSPC128 0.3955NM_006187 OAS3 0.3954NM_001139 ALOX12B 0.3953NM_153635 LOC151835 0.3953NM_030772 GJA10 0.3953NM_000789 ACE 0.3952NM_005711 EDIL3 0.3952NM_173475 MGC48972 0.3952NM_015977 WBSCR14 0.3951NM_020794 KIAA1365 0.395NM_178519 FLJ39421 0.395NM_030615 KIF25 0.395AB018324 SIK2 0.3949NM_018296 FLJ11004 0.3949NM_153024 FLJ31393 0.3949NM_032035 C14orf141 0.3948AK024689 AK024689 0.3948AK023598 LEPR 0.3948NM_002781 PSG5 0.3947NM_016076 PNAS-4 0.3946Contig55562_RC 0.3943

NM_139248 LIPH 0.3942Contig51953_RC 0.3941NM_005240 ETV3 0.394Contig52647_RC FLJ10312 0.3939NM_014686 KIAA0355 0.3939NM_002906 RDX 0.3938Contig44076_RC 0.3937AF052103 0.3937Contig38736_RC 0.3936NM_005808 C3orf8 0.3935AK002016 LOC196996 0.3935AB002373 RUSC2 0.3933NM_004790 SLC22A6 0.3933NM_021936 PLAC3 0.3933AF075103 AF075103 0.3932RSE_00000220256 RSE_0000022025 0.393NM_004605 SULT2B1 0.3928NM_021137 TNFAIP1 0.3928NM_133264 WIRE 0.3928NM_007211 C12orf2 0.3927Contig57290_RC 0.3927NM_017739 FLJ20277 0.3927Contig21039_RC 0.3926Contig54258_RC RAB21 0.3924NM_001264 CDSN 0.3924NM_004139 LBP 0.3923NM_152561 FLJ32205 0.3922NM_153361 MGC42105 0.3922ENST00000298035 LOC220213 0.3921NM_022648 TNS 0.392Contig13690_RC 0.3919NM_020364 DAZ3 0.3919Contig11032_RC 0.3918AB011542 EGFL5 0.3918Contig9053_RC 0.3917Contig41973_RC 0.3917NM_153449 SLC2A14 0.3917L08240 OATL1 0.3916NM_178512 FLJ37201 0.3916AL133117 THOC2 0.3915NM_004263 SEMA4F 0.3915NM_025149 FLJ20920 0.3915NM_153014 FLJ30634 0.3914Contig33979_RC 0.3913NM_000823 GHRHR 0.3913NM_148172 PEMT 0.3913Contig34789 PAG 0.3912NM_004781 VAMP3 0.3912NM_012409 PRND 0.3912NM_152520 FLJ25270 0.3908NM_153330 DNAJB8 0.3907

2

2

NM_005283 XCR1 0.3907AF169158 CABP3 0.3906NM_033303 ADRA1A 0.3905Contig46218_RC 0.3904NM_024834 FLJ13081 0.3904NM_000675 ADORA2A 0.3903AL122077 DNAH17 0.3903NM_006717 SPIN 0.3903NM_032998 DEDD 0.3903ENST00000298809 ENST0000029880 0.3902NM_145308 LOC220070 0.3898NM_002977 SCN9A 0.3897NM_006945 SPRR2B 0.3896NM_003582 DYRK3 0.3896NM_015497 DKFZP564G202 0.3896Contig36977_RC 0.3895Contig8223_RC DKFZp779M065 0.3894Contig46002_RC CRK7 0.3894NM_002017 FLI1 0.3894Contig62901_RC KIAA2026 0.3894NM_001429 EP300 0.3894Contig30499_RC 0.3893NM_024830 FLJ12443 0.3893AF038190 HMGA2 0.3892AB033049 KIAA1223 0.3892NM_013449 BAZ2A 0.3892NM_006403 NEDD9 0.3892NM_012395 PFTK1 0.3891NM_171982 TRIM35 0.3891NM_019055 ROBO4 0.389NM_020895 KIAA1533 0.389NM_182658 C20orf185 0.3889NM_014035 SNX24 0.3889NM_007037 ADAMTS8 0.3889AK022898 LOC149351 0.3888NM_138693 KLF14 0.3888NM_021969 NR0B2 0.3887ENST00000296361 ENST0000029636 0.3886ENST00000279503 ENST0000027950 0.3885NM_153455 C21orf87 0.3885NM_001830 CLCN4 0.3884NM_002508 NID 0.3884NM_006802 SF3A3 0.3884Contig46015_RC CALCRL 0.3883NM_173640 FLJ40906 0.3882NM_016160 APLP2 0.3881NM_032162 AKAP13 0.3881AL157458 AL157458 0.3881NM_000575 IL1A 0.3878AF086156 BPAG1 0.3877Contig20623_RC 0.3876

Contig16740_RC 0.3876NM_032144 RAB6C 0.3876NM_004599 SREBF2 0.3876NM_173647 RNF149 0.3875AF088735 TCRBV20S1 0.3873NM_080740 SUHW1 0.3873NM_001406 EFNB3 0.3873NM_000729 CCK 0.3872D42043 RAFTLIN 0.3871NM_005680 TAF1B 0.3871NM_175892 NM_175892 0.3871NM_000320 QDPR 0.387NM_025198 LOC80298 0.387Contig3057_RC BMPR2 0.3868NM_006035 CDC42BPB 0.3868AF087965 AF087965 0.3867NM_003281 TNNI1 0.3867NM_001760 CCND3 0.3866NM_003737 PCDH16 0.3866NM_003088 FSCN1 0.3865NM_014134 PRO0628 0.3865NM_181615 KRTAP20-1 0.3865NM_018059 FLJ10324 0.3864NM_000076 CDKN1C 0.3861NM_003056 SLC19A1 0.386Contig35510_RC OBSCN 0.3859Contig42631_RC 0.3858NM_030658 DKFZP564D166 0.3858NM_153008 FLJ30277 0.3858NM_005282 GPR4 0.3858NM_019009 TOLLIP 0.3857NM_014469 HNRNPG-T 0.3856NM_133498 SPACA4 0.3856NM_020806 GPHN 0.3856NM_014583 LMCD1 0.3854Contig37188_RC 0.3853NM_000025 ADRB3 0.3852NM_003055 SLC18A3 0.3851NM_152459 MGC45438 0.3851NM_003036 SKI 0.385Contig47741_RC DPP6 0.385NM_003278 TNA 0.3849NM_014892 RBM16 0.3849NM_021191 NEUROD4 0.3848NM_014228 SLC6A7 0.3848AL117542 DGAT2 0.3847NM_014729 TOX 0.3846NM_006811 TDE1 0.3846NM_175610 TJP1 0.3844Contig6352_RC 0.3843NM_001632 ALPP 0.3842

ENST00000301718 ENST000003017 0.3842NM_002019 FLT1 0.3842NM_015371 HS322B1A 0.3842NM_004170 SLC1A1 0.3841NM_182541 MGC39655 0.3841NM_018699 PRDM5 0.384NM_002889 RARRES2 0.384NM_175571 hIAN6 0.384NM_138385 LOC92305 0.384NM_148914 WBSCR21 0.3839NM_012470 TNPO3 0.3839NM_012302 LPHN2 0.3838NM_153345 FLJ90586 0.3835NM_133456 APXL2 0.3834AJ132820 tMDC II 0.3832NM_138460 CKLFSF5 0.3831NM_024047 NUDT9 0.3831NM_003183 ADAM17 0.383Contig45279_RC 0.3829NM_015854 LOC51036 0.3828NM_173525 MGC34805 0.3828NM_012114 CASP14 0.3827AK000205 AK000205 0.3827NM_014261 TRIF 0.3827Contig14461_RC 0.3826NM_014790 KIAA0555 0.3826NM_016598 ZDHHC3 0.3826NM_001348 DAPK3 0.3825NM_001094 ACCN1 0.3824AK058116 RNF148 0.3823NM_031271 TEX15 0.3823NM_152467 KLHL10 0.3823NM_021196 SLC4A5 0.3823NM_003798 CTNNAL1 0.3822NM_022113 KIF13A 0.3822Contig9042_RC 0.3821AK057669 PURB 0.3821NM_017534 MYH2 0.382NM_021073 BMP5 0.382NM_014867 KIAA0711 0.3818NM_147196 TMIE 0.3818Contig46329_RC 0.3817AA485939_RC 0.3817NM_005133 RCE1 0.3817Z70717 Z70717 0.3817NM_018704 DKFZp547A023 0.3817NM_014694 KIAA0605 0.3815NM_178339 APRG1 0.3815NM_006438 COLEC10 0.3814NM_002856 PVRL2 0.3814NM_080431 ARPM2 0.3813

AK056448 SYT6 0.3813Contig21391_RC 0.3812Contig17288_RC SNX25 0.3812Contig26096_RC 0.3811Contig23809_RC 0.3811NM_033341 BIRC8 0.3809AK022321 AK022321 0.3808ENST00000301456 FLJ46061 0.3807NM_054034 FN1 0.3806NM_021016 PSG3 0.3806Contig43416_RC 0.3804NM_024115 MGC4309 0.3804NM_152901 PYC1 0.3804NM_130777 GAGED3 0.3804NM_004485 GNG4 0.3803NM_004272 HOMER1 0.3803AB033063 KIAA1237 0.3803NM_002243 KCNJ15 0.3802AK001125 AK001125 0.3802Contig55770_RC GSK3B 0.38NM_173682 FLJ40288 0.38NM_018370 FLJ11259 0.3799NM_181847 AMIGO2 0.3799NM_002277 KRTHA1 0.3799NM_001400 EDG1 0.3798AF088056 FLJ13611 0.3798NM_000448 RAG1 0.3797NM_003734 AOC3 0.3795NM_016348 C5orf4 0.3794Contig34823_RC 0.3794NM_005007 NFKBIL1 0.3793NM_015852 H-plk 0.3793NM_015259 ICOSL 0.3792NM_023074 FLJ12644 0.379NM_032552 DAB2IP 0.3789NM_002976 SCN7A 0.3789Contig28760_RC 0.3787AK057672 AK057672 0.3787NM_174920 LOC201191 0.3784NM_015002 FBXO21 0.3784Contig46942_RC SKIP 0.3783NM_001821 CHML 0.3783AL390143 0.3781ENST00000289853 ENST0000028985 0.3781Contig36081_RC C14orf43 0.378NM_004428 EFNA1 0.3779NM_174906 MGC39724 0.3778NM_181539 KRT25B 0.3778NM_000527 LDLR 0.3776NM_015281 KIAA1043 0.3776NM_006238 PPARD 0.3774

NM_004982 KCNJ8 0.3774AB011146 KIAA0574 0.3773NM_001356 DDX3X 0.3771NM_005989 AKR1D1 0.377NM_005630 SLC21A2 0.377NM_016390 HSPC109 0.377NM_004723 ARHGEF2 0.377NM_004126 GNG11 0.3768NM_025185 DKFZP564D166 0.3767Contig43586_RC LOC134218 0.3766NM_006991 ZNF197 0.3766AB007932 PLXNA2 0.3765T83642_RC 0.3765Contig30372_RC 0.3765NM_170699 GPBAR1 0.3765NM_138611 HCC-4 0.3765NM_004504 HRB 0.3763NM_152794 HIF3A 0.3762NM_138329 NALP6 0.3762NM_001174 ARHGAP6 0.376NM_020384 CLDN2 0.376AK026267 AK026267 0.376NM_139056 ADAMTS16 0.376NM_181712 NM_181712 0.3758Contig8685_RC 0.3757NM_002606 PDE9A 0.3756U61096 U61096 0.3756NM_021195 CLDN6 0.3756NM_001542 IGSF3 0.3756NM_144690 FLJ30927 0.3755NM_020663 ARHJ 0.3754NM_032303 MGC10940 0.3753NM_152407 GRPEL2 0.3753Contig14555_RC 0.3752Contig24417_RC MGC12909 0.3751AB011145 TXNDC4 0.375NM_002224 ITPR3 0.375NM_015296 DOCK9 0.3749NM_014664 N4BP1 0.3748AK026868 AK026868 0.3748NM_015645 C1QTNF5 0.3748U00946 PRKWNK1 0.3746NM_003347 UBE2L3 0.3746NM_000180 GUCY2D 0.3745NM_000258 MYL3 0.3744ENST00000284286 ENST0000028428 0.3744NM_003018 SFTPC 0.3743Contig47178_RC 0.3743NM_006622 SNK 0.3742NM_022361 POPDC3 0.3742NM_014338 PISD 0.3742

NM_004099 STOM 0.3742NM_005730 CTDSP2 0.3741NM_032147 USP44 0.3741Contig44124_RC 0.3739NM_002368 MAGEB3 0.3739AK026701 AK026701 0.3739NM_138791 LOC122945 0.3738NM_005601 NKG7 0.3737NM_030756 TCF7L2 0.3737NM_152688 SLM1 0.3737NM_012468 TCL6 0.3736NM_000451 SHOX 0.3735Contig56160_RC 0.3733NM_182830 MAMDC1 0.3732NM_000080 CHRNE 0.3732Contig7974_RC 0.373NM_002885 RAP1GA1 0.3728NM_006225 PLCD1 0.3726AL161977 PCTK3 0.3726NM_007200 AKAP13 0.3726Contig81_RC ILF1 0.3725NM_021188 ZNF410 0.3725NM_173613 FLJ35785 0.3725NM_004579 MAP4K2 0.3724NM_172234 IL17RB 0.3724ENST00000300664 ENST0000030066 0.372AB051514 KIAA1727 0.372NM_153815 RASGRF1 0.3719NM_006212 PFKFB2 0.3719NM_016524 LOC51760 0.3717ENST00000277224 ENST0000027722 0.3717NM_002469 MYF6 0.3716AL137668 0.3715NM_032663 USP30 0.3715AK022342 CPN2 0.3714NM_018157 hSyn 0.3712NM_181532 ERAS 0.3712NM_023935 C20orf116 0.3712NM_012296 GAB2 0.3711NM_000063 C2 0.3711Contig41983_RC 0.3711NM_173557 RNF152 0.3711NM_004956 ETV1 0.371Contig44492_RC TOX 0.3709ENST00000297789 TRAM1 0.3709NM_152451 FLJ30973 0.3708NM_153371 PDZRN1 0.3708NM_001904 CTNNB1 0.3706Contig47225_RC 0.3706NM_020348 CNNM1 0.3706NM_022061 MRPL17 0.3706

AK000311 CRKL 0.3705NM_022482 ZNF336 0.3705NM_000738 CHRM1 0.3703NM_015930 TRPV2 0.3702AB007964 KIAA0495 0.3702Contig32344_RC 0.3702ENST00000298744 ENST0000029874 0.3702AF035315 ATP8A1 0.3701NM_080675 SPAG4L 0.3701NM_033046 RTKN 0.37NM_003765 STX10 0.37NM_020870 SH3MD2 0.37NM_002371 MAL 0.3699NM_016525 UBAP1 0.3699NM_173091 NFATC2 0.3699NM_006390 IPO8 0.3697NM_173648 NM_173648 0.3697NM_178543 LOC339221 0.3696NM_032664 FUT10 0.3695M65066 PRKAR1B 0.3695NM_012369 OR2F1 0.3695NM_000128 F11 0.3695NM_174946 NM_174946 0.3695NM_018320 RNF121 0.3691AK000160 MYO1B 0.369Contig37267_RC ST7OT1 0.369NM_003227 TFR2 0.369NM_006095 ATP8A1 0.369D80004 KIAA0182 0.3689ENST00000282639 ENST0000028263 0.3689AK074231 AK074231 0.3689NM_031362 COL4A3 0.3689NM_016201 AMOTL2 0.3688NM_006096 NDRG1 0.3688NM_016730 FOLR1 0.3688NM_002102 GYPE 0.3686NM_182774 FLJ36525 0.3686NM_170663 MINK 0.3684AK021752 LOC283464 0.3683NM_022747 C14orf136 0.3682AF486844 CASP12P1 0.3681NM_032310 MGC11115 0.3681NM_016946 F11R 0.368NM_032091 PCDHGA11 0.368NM_001441 FAAH 0.3679NM_032012 C9orf5 0.3676ENST00000275162 C6orf212 0.3675NM_025107 MYCT1 0.3675NM_130391 PTPRD 0.3674NM_020428 CTL2 0.3674NM_002656 PLAGL1 0.3673

NM_032758 PHF5A 0.3673NM_182642 CTDSP1 0.3673NM_006079 CITED2 0.3673NM_001050 SSTR2 0.3671NM_001398 ECH1 0.3671NM_014734 KIAA0247 0.3671NM_003490 SYN3 0.3671NM_001657 AREG 0.367NM_032639 FAPP2 0.3669Contig38744_RC 0.3668NM_078487 CDKN2B 0.3668NM_000802 FOLR1 0.3666NM_000453 SLC5A5 0.3665NM_153444 JCG3 0.3665Contig54142_RC DCBLD1 0.3663NM_003835 RGS9 0.3663Contig49341_RC ZDHHC9 0.3662Contig36872_RC SNX22 0.3661AL050297 LOC203069 0.3661NM_014868 RNF10 0.3661NM_004490 GRB14 0.3659Contig48270_RC LOC144097 0.3659NM_006874 ELF2 0.3659NM_020871 NM_020871 0.3658NM_032329 ING5 0.3658NM_018962 DSCR6 0.3657NM_006708 GLO1 0.3657NM_017549 UCC1 0.3656ENST00000281770 ENST000002817 0.3655NM_012165 FBXW3 0.3655NM_173641 FLJ33655 0.3655AK000216 ZDHHC3 0.3654NM_022463 NXN 0.3654NM_003028 SHB 0.3654Contig21271_RC RPLP2 0.3653NM_152264 SLC39A13 0.3652NM_024812 BAALC 0.3651NM_031899 GORASP1 0.3651NM_178511 LOC255783 0.365NM_017767 SLC39A4 0.3647NM_031993 PCDHGA1 0.3647NM_005272 GNAT2 0.3645NM_003692 TMEFF1 0.3644Contig34748_RC 0.3644NM_052896 CSMD2 0.3644NM_000387 SLC25A20 0.3643Contig32055_RC SRL 0.3642NM_181425 FRDA 0.3641NM_172350 MCP 0.3641NM_153204 C21orf90 0.364NM_006256 PRKCL2 0.3639

0

NM_003955 SOCS3 0.3639NM_144628 C20orf140 0.3638NM_006011 SIAT8B 0.3638AK023426 BMPR2 0.3637Contig22194_RC TEGT 0.3636NM_004787 SLIT2 0.3635NM_005203 COL13A1 0.3635NM_024078 MGC3162 0.3635Contig23991_RC KIAA1171 0.3634NM_005332 HBZ 0.3634NM_054033 FKBP1B 0.3634NM_014468 VENTX2 0.3633NM_181617 KRTAP21-2 0.3632NM_152710 C10orf27 0.3632NM_138435 LOC113828 0.3631NM_007174 CIT 0.3631NM_032272 MAF1 0.3631ENST00000245662 BTBD4 0.363NM_033656 WDR9 0.363NM_012403 ANP32C 0.363NM_144972 MGC23940 0.363NM_020777 SORCS2 0.3629NM_002633 PGM1 0.3628Contig50855_RC SLIT2 0.3628NM_000373 UMPS 0.3628NM_006210 PEG3 0.3628AB007970 0.3627AK056115 AK056115 0.3627NM_173505 LOC147463 0.3626Contig34108_RC 0.3625ENST00000282422 DKFZp313M072 0.3625NM_014617 CRYGA 0.3622NM_148896 NPB 0.3621NM_015547 THEA 0.3621NM_003963 TM4SF5 0.362NM_005326 HAGH 0.362NM_000066 C8B 0.3619NM_022003 FXYD6 0.3619NM_053278 GPR102 0.3618NM_022567 NYX 0.3617NM_024907 FBXO26 0.3617NM_005556 KRT7 0.3616ENST00000301730 RNPS1 0.3616NM_032582 USP32 0.3615NM_021115 SEZ6L 0.3615NM_003994 KITLG 0.3614NM_152579 FLJ38564 0.3614NM_177437 TAS2R60 0.3613Contig438_RC FLJ00133 0.3612NM_014360 NKX2-8 0.3612NM_001035 RYR2 0.3612

NM_025080 ASRGL1 0.3612NM_014348 POM121L1 0.3612NM_007368 RASA3 0.361NM_152339 MGC26885 0.361NM_022572 MR-1 0.361NM_004094 EIF2S1 0.361Contig54676_RC FNBP2 0.3609NM_018145 FLJ10579 0.3609AL137332 DRCTNNB1A 0.3609AJ406945 KRTAP4-15 0.3609NM_032173 FLJ12747 0.3609NM_152596 MGC33637 0.3609NM_080823 SRMS 0.3609NM_001735 C5 0.3608AL137436 SPTBN5 0.3607AK001590 C14orf132 0.3606Contig36888_RC 0.3606AB029030 KIAA1107 0.3606NM_005910 MAPT 0.3605NM_002853 RAD1 0.3605NM_058163 DT1P1A10 0.3605Contig1034_RC YY1 0.3602ENST00000300499 ENST0000030049 0.3602NM_033312 CDC14A 0.3602NM_016267 VGLL1 0.3601NM_152608 FLJ35382 0.3601NM_145762 GFRA4 0.36NM_152696 HIPK1 0.3599NM_007309 DIAPH2 0.3599Contig53364_RC SCN7A 0.3598ENST00000295445 ENST0000029544 0.3598NM_032883 C20orf100 0.3598NM_054021 GPR101 0.3597NM_003928 CXX1 0.3596AL162042 0.3596NM_152704 FLJ25477 0.3596NM_152933 PPEF2 0.3596NM_003669 INE1 0.3594NM_018379 FLJ11280 0.3594NM_000574 DAF 0.3593NM_001104 ACTN3 0.3593NM_177998 OTOP1 0.3593Contig28762_RC 0.3592NM_023089 CAPN10 0.3592NM_005104 BRD2 0.3592NM_001240 CCNT1 0.3591Contig47966_RC TCF7L2 0.359Contig50368 0.359NM_018687 LOC55908 0.3587RSE_00000885112 RSE_000008851 0.3587NM_153481 IL17RE 0.3587

e

NM_003042 SLC6A1 0.3587NM_006651 CPLX1 0.3587Contig41210_RC 0.3586NM_173573 MGC35138 0.3586NM_007222 ZHX1 0.3584AF038202 STX6 0.3582NM_173499 MGC44294 0.3582Contig41877_RC 0.3581Contig63302_RC 0.3581NM_024585 FLJ22160 0.3581NM_005535 IL12RB1 0.3579NM_004247 U5-116KD 0.3579NM_153324 DEFB123 0.3579AK001134 PCDH17 0.3578NM_138424 KIF12 0.3578NM_014398 LAMP3 0.3577Contig773 FLJ20989 0.3576ENST00000296148 KIAA0540 0.3576Contig27456_RC C14orf114 0.3575Contig475_RC 0.3574ENST00000297039 LOC285924 0.3574NM_152737 MGC33993 0.3574NM_022087 GALNT11 0.3573AA598803_RC LOC139886 0.3572NM_001977 ENPEP 0.3572NM_054024 MIA2 0.3572NM_004138 KRTHA3A 0.3571Contig12356_RC NEBL 0.3571NM_002340 LSS 0.3571NM_003813 ADAM21 0.3571NM_152614 MGC35154 0.3571NM_032391 PRAC 0.357NM_005592 MUSK 0.357Contig54961_RC KPI2 0.3569X86141 IGHG3 0.3569AF086164 AF086164 0.3568NM_032648 MGC10820 0.3568NM_145202 UPA 0.3567NM_002569 FURIN 0.3566NM_003259 ICAM5 0.3566Contig23085_RC 0.3565NM_015278 SASH1 0.3563Contig27840_RC 0.3562AK002036 0.3561AL365410 hypothetical prot 0.356NM_032327 ZDHHC16 0.356NM_004540 NCAM2 0.356AB020633 KIAA0826 0.3558NM_014961 RIPX 0.3558NM_016057 COPZ1 0.3558NM_000570 FCGR3B 0.3557

Contig55048_RC JAM2 0.3556NM_004558 NRTN 0.3556NM_014235 UBL4 0.3556NM_015230 CENTD1 0.3556NM_020684 NPD007 0.3555Contig45634_RC LOC286073 0.3555NM_002543 OLR1 0.3555NM_017805 FLJ20401 0.3554NM_006934 SLC6A9 0.3554NM_001544 ICAM4 0.3554NM_001856 COL16A1 0.3554NM_052953 LRP15 0.3552NM_012107 BP75 0.3551NM_012283 KCNG2 0.3551NM_000400 ERCC2 0.3551RSE_00000113972 RSE_0000011397 0.355NM_005828 HAN11 0.355Contig36447 0.3549NM_024092 MGC5508 0.3549NM_031477 MGC10500 0.3549NM_021013 KRTHA4 0.3548NM_031889 ENAM 0.3548NM_005109 OSR1 0.3548Contig43189_RC CCBP2 0.3546NM_001891 CSN2 0.3546NM_016948 PARD6A 0.3544NM_013387 HSPC051 0.3544NM_015331 NCSTN 0.3544Contig22775_RC MOB3B 0.3543NM_012261 C20orf103 0.3543NM_020448 DJ462O23.2 0.3543AK074181 FLJ00254 0.3542NM_016062 CGI-128 0.3542Contig36512_RC 0.3541NM_020770 CGN 0.3541NM_021213 PCTP 0.354NM_005626 SFRS4 0.354ENST00000279058 ENST0000027905 0.3539ENST00000290220 ENST0000029022 0.3539NM_032764 MGC16153 0.3539Contig46464_RC 0.3538NM_004881 TP53I3 0.3538NM_007292 ACOX1 0.3536NM_006540 NCOA2 0.3536NM_152544 FLJ35725 0.3535NM_006876 B3GNT6 0.3534AK023357 IGSF4 0.3534NM_001230 CASP10 0.3533Contig19210_RC 0.3532NM_002835 PTPN12 0.3532NM_018328 MBD5 0.3532

NM_004206 SEC22L3 0.3532NM_173538 FLJ35802 0.3531Contig8371_RC 0.353Contig20839_RC 0.353NM_004803 ORCTL4 0.3529Contig52305_RC 0.3528NM_022744 FLJ13868 0.3528NM_024933 FLJ12056 0.3528NM_024882 C6orf155 0.3527NM_004581 RABGGTA 0.3527NM_004609 TCF15 0.3525NM_014899 RHOBTB3 0.3525ENST00000300766 ENST0000030076 0.3525NM_002026 FN1 0.3525Contig26713_RC 0.3524AL117448 RAB6IP1 0.3524NM_006721 ADK 0.3523AF086538 AF086538 0.3523NM_145012 C10orf9 0.3523AK025893 AK025893 0.3522NM_014817 KIAA0644 0.3521NM_005195 CEBPD 0.3521AB067489 FHOD2 0.3521NM_181624 KRTAP23-1 0.3521AI681039_RC 0.352NM_020644 C11orf15 0.352NM_006041 HS3ST3B1 0.3518NM_177478 MTF 0.3518NM_004765 BCL7C 0.3517NM_000578 SLC11A1 0.3517NM_006965 ZNF24 0.3516NM_148887 MRPL10 0.3516NM_019850 NGEF 0.3516AL117578 C21orf30 0.3514NM_144990 FLJ23878 0.3514NM_004959 NR5A1 0.3514NM_032250 DKFZp434A171 0.3514Contig11106_RC 0.3513NM_175064 WBSCR19 0.3513NM_016942 OR51A1P 0.3512ENST00000287819 ENST000002878 0.3512AK056904 AK056904 0.3512ENST00000248346 ENST0000024834 0.3511NM_181521 CKLFSF4 0.3511NM_153228 FLJ38335 0.3511NM_005697 SCAMP2 0.3511NM_153446 GALGT2 0.351NM_181809 BMP8A 0.351Contig5471_RC 0.3509NM_003802 MYH13 0.3509NM_005938 MLLT7 0.3508

NM_002402 MEST 0.3507NM_033419 CAB2 0.3507NM_004390 CTSH 0.3505NM_002338 LSAMP 0.3505NM_001460 FMO2 0.3505Contig53623_RC 0.3504NM_003949 HAP1 0.3504NM_020438 DOLPP1 0.3504AK023330 ANXA1 0.3502Contig53223 PPP1R15B 0.35NM_001089 ABCA3 0.3498NM_080474 SERPINB12 0.3498Contig48621_RC C6orf189 0.3497NM_001959 EEF1B2 0.3497NM_004831 CRSP7 0.3497NM_016138 COQ7 0.3497NM_024895 FLJ23209 0.3496NM_004807 HS6ST1 0.3496NM_031866 FZD8 0.3495NM_004019 DMD 0.3493Contig23881_RC 0.3492NM_002189 IL15RA 0.3492NM_020778 MIDORI 0.3491NM_000786 CYP51A1 0.349NM_001049 SSTR1 0.349ENST00000292772 ENST000002927 0.3489NM_080657 cig5 0.3489NM_144626 MGC17299 0.3489NM_001972 ELA2 0.3488Contig48021_RC FLJ37034 0.3487NM_007347 AP4E1 0.3487NM_017816 LYAR 0.3486NM_000361 THBD 0.3486NM_014437 SLC39A1 0.3486NM_005450 NOG 0.3486NM_006568 CGRRF1 0.3483Contig46375_RC 0.3482NM_003529 HIST1H3A 0.3482NM_002854 PVALB 0.3481NM_002676 PMM1 0.3481NM_023938 MGC2742 0.3481NM_006856 ATF7 0.3481NM_001817 CEACAM4 0.348NM_022772 EPS8L2 0.348NM_004726 REPS2 0.348NM_004378 CRABP1 0.3479ENST00000256580 ENST0000025658 0.3479NM_022075 LASS2 0.3478Contig37562_RC PARD6B 0.3476Contig42215_RC 0.3475NM_003564 TAGLN2 0.3473

NM_182617 LOC348158 0.3473NM_004621 TRPC6 0.3472NM_000221 KHK 0.3472NM_000237 LPL 0.3471NM_000561 GSTM1 0.3471NM_016234 FACL5 0.3471NM_145254 LOC124491 0.3471NM_003003 SEC14L1 0.3471NM_015595 DKFZP434D146 0.3471NM_144582 TEX261 0.3471AL117587 KIAA1281 0.347ENST00000259355 ENST0000025935 0.347AF070554 TCEB3 0.3469Contig55448_RC BAIAP1 0.3468AK023152 FLJ10099 0.3468NM_153030 FLJ31958 0.3468NM_000209 IPF1 0.3468NM_003337 UBE2B 0.3468NM_001420 ELAVL3 0.3467Contig49462_RC KIAA0211 0.3467Contig44736 PROX1 0.3467NM_002827 PTPN1 0.3466NM_052961 SLC26A8 0.3466NM_005225 E2F1 0.3465Contig729_RC RIN2 0.3464Contig26648_RC LOC123722 0.3464NM_022481 ARAP3 0.3464NM_024539 RNF128 0.3462NM_014420 DKK4 0.3462NM_012234 RYBP 0.3462NM_002622 PFDN1 0.3462U37028 ITGAD 0.3461NM_025003 ADAMTS20 0.3461RSE_00000869609 RSE_0000086960 0.346NM_176814 LOC168850 0.3459NM_024342 GRLF1 0.3459NM_003944 SELENBP1 0.3458NM_001347 DGKQ 0.3458NM_020215 C14orf132 0.3457NM_003364 UPP1 0.3455NM_033168 B3GALT3 0.3455NM_012330 MYST4 0.3455AK057857 AK057857 0.3454NM_024761 MOB3B 0.3454Contig28045_RC 0.3453NM_003149 STAC 0.3452NM_173728 ARHGEF15 0.3452Contig35108_RC 0.3451NM_004444 EPHB4 0.3451NM_024560 FLJ21963 0.3451NM_003604 IRS4 0.345

Contig26766_RC IGSF4 0.3449NM_031953 SNX25 0.3449NM_018553 HSA277841 0.3449Contig29373_RC 24b2/STAC2 0.3448Contig32538_RC HNRPA0 0.3448NM_003654 CHST1 0.3447NM_015725 RDH8 0.3447Contig32137_RC 0.3445NM_182594 ZNF454 0.3445Contig8926_RC LHFPL3 0.3444AL049370 UNC5B 0.3444NM_145865 FLJ38819 0.3444NM_145747 TXNRD2 0.3444NM_032209 FLJ21777 0.3443NM_020137 GRIPAP1 0.3443NM_024046 MGC8407 0.3442NM_031445 MGC4268 0.3442NM_000735 CGA 0.3441NM_000118 ENG 0.344NM_016522 HNT 0.3439NM_024640 FLJ23476 0.3438NM_014470 RHO6 0.3437Contig20749_RC 0.3435AJ406929 KAP2.1B 0.3435NM_013362 ZNF225 0.3435NM_018993 RIN2 0.3435Contig40232_RC LAT1-3TM 0.3434AF131827 HMGCLL1 0.3433NM_006326 NIFIE 14 0.3433ENST00000281047 ENST0000028104 0.3433NM_138612 HAS3 0.3433AF052107 LOC129642 0.3432NM_014418 TCL6 0.3432NM_153036 C6orf78 0.3432NM_004690 LATS1 0.3431NM_000385 AQP1 0.343NM_004617 TM4SF4 0.343ENST00000267406 ENST0000026740 0.343NM_006766 MYST3 0.3429Contig43338_RC FLJ32535 0.3429NM_178168 JCG6 0.3429NM_005477 HCN4 0.3429NM_014735 PHF16 0.3428NM_181686 KRTAP12-1 0.3428NM_000914 OPRM1 0.3428Contig32798_RC 0.3427NM_006465 DRIL2 0.3427NM_003260 TLE2 0.3426NM_003215 TEC 0.3425NM_004073 CNK 0.3424NM_080872 UNC5D 0.3424

AK056629 MCOLN1 0.3423Contig47102_RC DKFZp761P0423 0.3421NM_139028 ADAMTS13 0.3421AK057297 AK057297 0.3419ENST00000300077 ENST000003000 0.3419NM_005819 STX6 0.3419NM_152335 FLJ38190 0.3419NM_178428 LEP9 0.3419NM_003609 HIRIP3 0.3419NM_018412 ST7 0.3418Contig41079_RC NCRMS 0.3416NM_002528 NTHL1 0.3415NM_005698 SCAMP3 0.3415NM_015250 BICD2 0.3415NM_022746 FLJ22390 0.3415NM_018725 IL17RB 0.3414NM_003531 HIST1H3C 0.3414Contig57903_RC SASH1 0.3413NM_001176 ARHGDIG 0.3413NM_006277 ITSN2 0.3413NM_014828 C14orf92 0.3413NM_003809 TNFSF12 0.3413Contig35186_RC SNRPE 0.3411NM_002928 RGS16 0.3411NM_032445 MEGF11 0.3411Contig25503_RC 0.341AK024121 LOC162073 0.341NM_145914 ZNF38 0.3409NM_017413 APLN 0.3409ENST00000271219 ENST000002712 0.3408NM_031894 FTHL17 0.3408NM_173452 FCN3 0.3408NM_004638 BAT2 0.3408NM_033018 PCTK1 0.3407NM_033167 B3GALT3 0.3407NM_032266 DKFZp434G118 0.3407NM_032054 PCDHGA5 0.3407NM_000390 CHM 0.3407NM_181715 LOC200186 0.3407NM_002216 ITIH2 0.3406AK026891 ZNF445 0.3405AL713738 IL7R 0.3405NM_032717 MGC11324 0.3405NM_003764 STX11 0.3405NM_004393 DAG1 0.3404Contig49013_RC MGC45731 0.3404NM_003445 ZNF155 0.3404NM_003602 FKBP6 0.3403NM_016341 PLCE1 0.3403NM_032227 FLJ22679 0.3402NM_178469 LOC138307 0.3402

NM_152554 C6orf195 0.3401NM_178868 CKLFSF8 0.3401NM_003879 CFLAR 0.3401NM_014879 GPR105 0.34NM_175068 K6IRS3 0.34NM_022347 IFRG15 0.34ENST00000293981 DKFZp761A132 0.3399NM_005430 WNT1 0.3399AB007925 FNBP2 0.3398NM_181791 GPR141 0.3398NM_000963 PTGS2 0.3397AL117605 SPTBN1 0.3397Contig52137_RC PFKFB2 0.3397Contig23386_RC DKFZP564O043 0.3397AL049312 AL049312 0.3397NM_005435 ARHGEF5 0.3397NM_001534 TLX2 0.3396NM_001935 DPP4 0.3396NM_005497 GJA7 0.3395NM_139280 ORMDL3 0.3395NM_005567 LGALS3BP 0.3395NM_000818 GAD2 0.3394NM_181647 RFT1 0.3394NM_020198 GK001 0.3393NM_018555 ZNF331 0.3392NM_002770 PRSS2 0.3392NM_080655 MGC17337 0.3392NM_031886 KCNA7 0.3392NM_013958 NRG1 0.3391NM_003728 UNC5C 0.339AK024135 AK024135 0.339ENST00000292523 KIAA0014 0.3389NM_052970 HSPA12B 0.3388NM_014322 OPN3 0.3387NM_002363 MAGEB1 0.3387NM_130844 WWOX 0.3386NM_033093 TRIM5 0.3386NM_006603 STAG2 0.3385NM_181738 PRDX2 0.3385NM_152525 FLJ25351 0.3385NM_021093 PYY2 0.3385NM_144578 C14orf32 0.3385NM_002869 RAB6A 0.3385NM_005239 ETS2 0.3384NM_004429 EFNB1 0.3384NM_153712 TTL 0.3384Contig45303_RC 0.3383NM_032461 SPANXB1 0.3383Contig55351_RC MGC45871 0.3382NM_014847 NICE-4 0.3382NM_001846 COL4A2 0.3382

NM_018582 PRO1483 0.3382NM_000069 CACNA1S 0.3381NM_030812 LOC81569 0.338NM_003240 EBAF 0.3379NM_004422 DVL2 0.3378NM_018065 FLJ10346 0.3378NM_032982 CASP2 0.3378NM_178861 ZNF183L1 0.3378NM_018113 LIMR 0.3375NM_001654 ARAF1 0.3375NM_001084 PLOD3 0.3374NM_032195 SON 0.3374NM_020457 THAP11 0.3374NM_021242 STRAIT11499 0.3373NM_004850 ROCK2 0.3373NM_001123 ADK 0.3373NM_032869 CML66 0.3373NM_013370 OKL38 0.3372NM_006788 RALBP1 0.3371AK056136 AK056136 0.3371NM_032676 MGC10955 0.3371Contig21742_RC 0.337AK026905 MICAL2 0.337NM_000496 CRYBB2 0.3369AB033112 BRPF3 0.3369NM_152860 SP7 0.3369NM_005654 NR2F1 0.3368Contig13822_RC 0.3367NM_000877 IL1R1 0.3366NM_017540 GALNT10 0.3366Contig9524_RC 0.3366Contig52186_RC ZFP67 0.3366NM_006553 HFL-EDDG1 0.3365NM_003636 KCNAB2 0.3365NM_000915 OXT 0.3364NM_006259 PRKG2 0.3364NM_003213 TEAD4 0.3363NM_017724 LRRFIP2 0.3363NM_147132 GALT 0.3363NM_032094 PCDHGA12 0.3363NM_017580 ZRANB1 0.3362Contig57908_RC C6orf145 0.3361AF087995 LMOD2 0.3361NM_017744 ST7L 0.3361AL122085 TSRC1 0.3358Contig31192_RC MYO7B 0.3358AK057259 AK057259 0.3358NM_024821 FLJ22349 0.3358NM_153379 KREMEN1 0.3358ENST00000300552 ENST0000030055 0.3357NM_032587 CARD6 0.3357

NM_002961 S100A4 0.3356Contig27060_RC 0.3356NM_005963 MYH1 0.3356RSE_00000873612 RSE_000008736 0.3356NM_174880 USH3A 0.3356NM_080924 LOC91219 0.3356NM_032951 WBSCR14 0.3356NM_182604 FLJ36131 0.3356U84511 U84511 0.3355NM_178275 DKFZp434B1231 0.3354NM_024636 FLJ23153 0.3354NM_003047 SLC9A1 0.3353NM_001496 GFRA3 0.3353Contig43853_RC 0.3352AF086477 AF086477 0.3351NM_005636 SSX4 0.3351NM_000531 OTC 0.3351Contig21869_RC C9orf4 0.335NM_005274 GNG5 0.335AA644150_RC 0.3349NM_052891 PGLYRPIalpha 0.3349NM_001658 ARF1 0.3349AK025053 AK025053 0.3348NM_032888 COL27A1 0.3348NM_174977 SEC14L4 0.3348Contig37397_RC 0.3347NM_012464 TLL1 0.3347NM_003781 B3GALT3 0.3347NM_006612 KIF1C 0.3346NM_004112 FGF11 0.3346NM_014952 KIAA0945 0.3346NM_004613 TGM2 0.3345NM_023013 LOC65121 0.3345AK023912 AK023912 0.3344NM_172211 CSF1 0.3344NM_032760 MGC14966 0.3344Contig40251_RC 0.3343NM_001675 ATF4 0.3343Contig30032_RC KIAA0960 0.3342NM_014907 KIAA0967 0.3342NM_013289 KIR3DL1 0.334ENST00000294746 ENST0000029474 0.334NM_020843 ZNF291 0.334NM_145245 LOC115704 0.3339NM_017617 NOTCH1 0.3339NM_013978 BNIP1 0.3338NM_024013 IFNA1 0.3338NM_006642 SDCCAG8 0.3338NM_020340 KIAA1244 0.3338Contig15216_RC 0.3337NM_014958 ARHGEF15 0.3336

NM_001874 CPM 0.3335NM_176811 NALP8 0.3335NM_014880 DCL-1 0.3334ENST00000298710 KIAA0599 0.3334NM_152478 FLJ31030 0.3334Contig46756_RC SYN2 0.3333NM_002903 RCV1 0.3331X77690 TIMP3 0.3331Contig27799_RC 0.333NM_020400 GPR92 0.333AK054894 TRAP240 0.333NM_014240 LIMD1 0.333NM_002657 PLAGL2 0.3328Contig49670_RC FGD6 0.3327NM_002272 KRT4 0.3327NM_032362 HEIL1 0.3327NM_170607 TCFL4 0.3327NM_153609 TMPRSS6 0.3327AL110139 0.3325NM_012262 HS2ST1 0.3325NM_152545 GPIG4 0.3324NM_032099 PCDHGB5 0.3324NM_002438 MRC1 0.3323Contig59481_RC KPNB1 0.3323NM_014186 HSPC166 0.3323NM_001323 CST6 0.3322NM_052954 CYYR1 0.3322AK027082 AK027082 0.3321Contig40389_RC 0.332NM_032833 PPP1R15B 0.332NM_030913 SEMA6C 0.3319NM_173484 FLJ40160 0.3319NM_003957 STK29 0.3319Contig1152_RC 0.3318NM_018719 RAM2 0.3318NM_014964 EPN2 0.3318NM_018463 MDS028 0.3318NM_024667 FLJ12750 0.3317NM_003271 TM4SF7 0.3316NM_080794 MRPL39 0.3316NM_001560 IL13RA1 0.3315NM_014885 APC10 0.3314AL355708 NEO1 0.3313Contig27275_RC 0.3313AK001008 0.3313AJ297964 TTY2 0.3313Contig36843_RC RBBP1 0.3311BC024006 BC024006 0.3311ENST00000274083 ENST0000027408 0.3311NM_024076 KCTD15 0.3311NM_033546 MRLC2 0.3311

9

4

8

NM_002144 HOXB1 0.331NM_006898 HOXD3 0.3309NM_015603 DKFZP586M101 0.3309NM_012098 ANGPTL2 0.3309NM_002004 FDPS 0.3308AK057905 KRTHB6 0.3308NM_005057 RBBP5 0.3308AF070580 0.3306NM_013422 ARHGAP6 0.3306NM_012264 C22orf5 0.3304Contig43083_RC FOS 0.3304Contig28952_RC 0.3303AB051487 DUSP16 0.3303Contig22749 0.3302NM_006202 PDE4A 0.3301AF086028 ERBB3 0.3301NM_014634 PPM1F 0.3301NM_007059 KPTN 0.3301Contig20807_RC 0.33NM_004778 GPR44 0.3299Contig43944_RC 0.3298AK055553 AK055553 0.3298NM_021098 CACNA1H 0.3298NM_014573 MAC30 0.3298NM_001357 DHX9 0.3297NM_173674 DCBLD1 0.3297Contig760_RC GSPT1 0.3296Contig25362_RC DKFZP566A152 0.3295NM_018373 SYNJ2BP 0.3295NM_022894 PAPOLG 0.3295NM_001771 CD22 0.3294NM_002571 PAEP 0.3294NM_003558 PIP5K1B 0.3293NM_002218 ITIH4 0.3293NM_032124 DKFZP564D137 0.3293NM_015613 LRRC21 0.3293NM_001054 SULT1A2 0.3292Contig40181_RC ALAD 0.3292ENST00000298903 ENST0000029890 0.3292AK057470 AK057470 0.3291ENST00000300671 ENST000003006 0.3291Contig36963_RC HSPC129 0.329NM_014905 GLS 0.3288NM_022449 RAB17 0.3288NM_032976 CASP10 0.3288NM_024334 MGC3222 0.3288Contig54180 PERQ1 0.3287ENST00000294945 ENST0000029494 0.3287NM_139047 MAPK8 0.3287Contig58339_RC FLJ21616 0.3286NM_016173 HEMK 0.3286

NM_033083 EAF1 0.3286NM_003150 STAT3 0.3285NM_032374 MGC2562 0.3285NM_032923 MGC16025 0.3285AK057786 AK057786 0.3284NM_031246 PSG2 0.3284NM_000804 FOLR3 0.3283NM_000610 CD44 0.3282NM_006309 LRRFIP2 0.3282NM_002691 POLD1 0.328AF009267 0.328NM_018903 PCDHA12 0.328NM_004915 ABCG1 0.3279NM_015238 KIBRA 0.3278NM_021221 LY6G5B 0.3275NM_014629 ARHGEF10 0.3275NM_020550 TCL6 0.3275NM_000229 LCAT 0.3274NM_004554 NFATC4 0.3273NM_001308 CPN1 0.3273NM_182528 LOC165257 0.3273AK022198 GALNT5 0.3272NM_001798 CDK2 0.3271NM_130388 ASB12 0.3271NM_015899 LOC51054 0.3271NM_133457 COL26A1 0.3271NM_147147 BVES 0.3271NM_013360 ZNF222 0.3271NM_031285 PP1057 0.327NM_145074 PRSS25 0.327AI373835_RC PLCE1 0.3269NM_000070 CAPN3 0.3269NM_080670 SLC35A4 0.3269NM_005411 SFTPA1 0.3269NM_002718 PPP2R3A 0.3268ENST00000298824 ENST0000029882 0.3268Contig42330_RC 0.3267AL080221 DOT1L 0.3267NM_003665 FCN3 0.3267NM_018334 LRRN3 0.3267NM_015488 MR-1 0.3267NM_006111 ACAA2 0.3265NM_000332 SCA1 0.3265Contig37262 0.3265AK021630 AK021630 0.3264NM_001986 ETV4 0.3264NM_005811 GDF11 0.3264NM_182535 LOC200261 0.3264NM_020184 CNNM4 0.3263NM_013343 LOH3CR2A 0.3263NM_001190 BCAT2 0.3263

AB033103 KIAA1277 0.3262NM_000344 SMN1 0.3262Contig35700_RC TWISTNB 0.3261NM_018908 PCDHA5 0.326NM_015716 MINK 0.326NM_016353 ZDHHC2 0.326NM_001845 COL4A1 0.326NM_002566 P2RY11 0.3259NM_173616 FLJ35894 0.3259NM_001850 COL8A1 0.3259NM_173856 VN1R2 0.3259NM_006662 SRCAP 0.3258NM_014067 LRP16 0.3258NM_016366 CABP2 0.3258AK000776 0.3257Contig46925 0.3257AB007946 PDE4DIP 0.3257NM_032491 RFX4 0.3257NM_001332 CTNND2 0.3257NM_001816 CEACAM8 0.3256Contig45841_RC 0.3255NM_024327 MGC2508 0.3255NM_014312 CTXL 0.3254AF361356 PR1 0.3254NM_002005 FES 0.3253NM_018286 FLJ10970 0.3253NM_006841 SLC38A3 0.3253NM_015582 DKFZP564B147 0.3252Contig25653_RC CDH11 0.3252NM_024017 HOXB9 0.3252ENST00000301630 ENST0000030163 0.3251AF086516 MGC22793 0.3251ENST00000289991 GAS8 0.3251AK022320 AK022320 0.3251NM_173468 MOB4A 0.325NM_003840 TNFRSF10D 0.325NM_001277 CHK 0.3247NM_030572 MGC10946 0.3246NM_002581 PAPPA 0.3246NM_001510 GRID2 0.3246NM_138632 HRIHFB2122 0.3245NM_004124 GMFB 0.3244NM_013381 TRHDE 0.3244M19668 TTN 0.3244NM_024862 FLJ13962 0.3244NM_007283 MGLL 0.3243NM_002686 PNMT 0.3243AL365404 GPR108 0.3243NM_172210 CSF1 0.3243NM_020866 KLHL1 0.3243NM_001822 CHN1 0.3242

Contig51621_RC USP13 0.3242Contig57896_RC DC-UbP 0.3242AK054829 AK054829 0.3242NM_016324 ZNF274 0.3242NM_014876 KIAA0063 0.3241NM_014464 TINAG 0.324NM_018907 PCDHA4 0.324AF039023 RANBP6 0.3239NM_021974 POLR2F 0.3239NM_182685 EFNA1 0.3239AK025352 MAST205 0.3238NM_130770 HTR3C 0.3238NM_015411 SUMF2 0.3238NM_033635 SCAND2 0.3238NM_003264 TLR2 0.3238NM_018654 GPRC5D 0.3238NM_006562 LBX1 0.3238Contig49768_RC PDE4D 0.3237NM_006963 ZNF22 0.3237NM_004353 SERPINH1 0.3236NM_002409 MGAT3 0.3236NM_001797 CDH11 0.3235NM_000189 HK2 0.3235NM_017689 FLJ20151 0.3235NM_005585 MADH6 0.3234NM_002918 RFX1 0.3234NM_152473 FLJ32214 0.3233NM_014184 HSPC163 0.3232NM_139322 ATRN 0.3232NM_000367 TPMT 0.3232AL110150 0.3231ENST00000244032 C20orf174 0.3231NM_000696 ALDH9A1 0.323NM_000923 PDE4C 0.3229NM_014674 EDEM1 0.3228Contig16481_RC 0.3228NM_000486 AQP2 0.3228NM_003118 SPARC 0.3228NM_153229 FLJ33318 0.3228NM_000716 C4BPB 0.3226NM_005137 DGCR2 0.3226NM_000629 IFNAR1 0.3226NM_031860 PCDHA10 0.3225NM_017960 FLJ20808 0.3225NM_002159 HTN1 0.3224NM_030927 DC-TM4F2 0.3224NM_032933 MGC11386 0.3224NM_022549 FEZ1 0.3224NM_003548 HIST2H4 0.3224NM_172108 KCNQ2 0.3224AB075856 KIAA1976 0.3223

NM_000940 PON3 0.3223NM_020998 MST1 0.3223NM_004349 CBFA2T1 0.3222NM_005194 CEBPB 0.3222NM_013306 SNX15 0.3222Contig28032_RC 0.3221NM_006064 RRAGB 0.3221NM_000330 RS1 0.322NM_152256 RAI1 0.322NM_012312 KIR2DS2 0.322AK055479 PRICKLE2 0.3219NM_018906 PCDHA3 0.3219Contig28098_RC 0.3218NM_021630 PDLIM2 0.3218NM_182577 FLJ40059 0.3218NM_007252 RPF-1 0.3218Contig45316_RC PTPLB 0.3217NM_003141 SSA1 0.3217Contig30146_RC 0.3216NM_004772 C5orf13 0.3215NM_152749 MGC33190 0.3215NM_018639 WSB2 0.3214Contig40600_RC 0.3214AF088020 AF088020 0.3213NM_020361 CPA6 0.3212Contig47895_RC 0.3212AB011136 KIAA0564 0.3212Contig42428_RC MGC17839 0.3211NM_018003 UACA 0.3211NM_001427 EN2 0.3208NM_001704 BAI3 0.3208NM_003733 OASL 0.3208AK024164 AK024164 0.3208NM_002299 LCT 0.3208NM_000703 ATP1A3 0.3207NM_002742 PRKCM 0.3207NM_031412 GABARAPL1 0.3207NM_170605 INADL 0.3207NM_002290 LAMA4 0.3206NM_153460 IL17RC 0.3206Contig38939_RC 0.3204AI076473_RC RIPX 0.3204Contig55764_RC KIAA2028 0.3204NM_019596 C21orf62 0.3204NM_018979 PRKWNK1 0.3204NM_002111 HD 0.3204NM_033247 PML 0.3203Contig27884_RC 0.3202NM_005803 FLOT1 0.32NM_020637 FGF22 0.32NM_018905 PCDHA2 0.32

AB018310 KIAA0767 0.3199Contig28181_RC 0.3197NM_016335 PRODH 0.3197NM_001258 CDK3 0.3196D87450 KIAA0261 0.3195NM_001045 SLC6A4 0.3195NM_000437 PAFAH2 0.3195NM_144767 AKAP13 0.3195NM_031912 SYT15 0.3195NM_017907 FLJ20625 0.3194NM_003463 PTP4A1 0.3194NM_018899 PCDHAC2 0.3194Contig43380_RC 0.3193NM_000964 RARA 0.3192NM_019839 LTB4R2 0.3192NM_181713 LOC165324 0.3192NM_032482 DOT1L 0.3192NM_182705 MGC45871 0.3191NM_006695 RPIP8 0.319NM_153250 RNF3 0.319Contig42022_RC CAPN3 0.3189AK022347 AK022347 0.3188AF130051 PRO0898 0.3188NM_018217 C20orf31 0.3188NM_178462 C20orf78 0.3188NM_001554 CYR61 0.3187Contig53674_RC GNAS 0.3187NM_016032 ZDHHC9 0.3187NM_148888 CCL25 0.3187NM_178453 MGC52282 0.3187NM_014188 HSPC182 0.3187NM_152638 MGC26598 0.3185NM_001605 AARS 0.3185NM_000678 ADRA1D 0.3185Contig14810_RC 0.3184ENST00000292000 ENST0000029200 0.3184NM_031439 SOX7 0.3184NM_181844 BCL6B 0.3184NM_002074 GNB1 0.3183NM_018902 PCDHA11 0.3182X65705 FXYD2 0.3182NM_033178 DUX4 0.3182NM_019618 IL1F9 0.3181NM_014152 HSPC054 0.3181NM_024120 C20orf7 0.318NM_144614 MBD3L2 0.318NM_172131 WFDC10B 0.318NM_178844 NOD3 0.318L13433 L13433 0.3179NM_181334 ARHGAP8 0.3179NM_145649 GCNT2 0.3176

2

NM_000112 SLC26A2 0.3175NM_002701 POU5F1 0.3175NM_001489 NR6A1 0.3175ENST00000293257 ENST0000029325 0.3174NM_031290 DKFZP434K117 0.3174NM_144627 SSTK-IP 0.3174NM_147148 GSTM4 0.3174NM_000479 AMH 0.3172ENST00000291710 ENST000002917 0.3172NM_012345 NUFIP1 0.317NM_130762 MADCAM1 0.317NM_018900 PCDHA1 0.317NM_016069 Magmas 0.317NM_000306 POU1F1 0.3169ENST00000288306 ENST0000028830 0.3169Contig45266 0.3168NM_148959 HUS1B 0.3168AK056930 IRF2BP2 0.3167NM_014066 ZNF7 0.3167NM_020678 HT017 0.3167AB033059 KIAA1233 0.3165Contig30692_RC 0.3165Contig8963_RC DDX46 0.3165Contig29754_RC 0.3164NM_014410 CLUL1 0.3163RSE_00000890621 RSE_0000089062 0.3163NM_000510 FSHB 0.3163AB040944 ZSWIM5 0.3162NM_005802 TOPORS 0.3162NM_005973 PRCC 0.3162NM_173050 SCUBE1 0.3162NM_139155 ADAMTS14 0.316Contig43410_RC 0.3159NM_018397 CHDH 0.3159NM_024508 ZBED2 0.3158NM_130442 ELMO1 0.3158AF086189 ALS2CR9 0.3157NM_002186 IL9R 0.3157NM_139205 HDAC5 0.3157NM_004055 CAPN5 0.3157Contig32345_RC 0.3156NM_000924 PDE1B 0.3156NM_032892 MGC14161 0.3156NM_003626 PPFIA1 0.3156Contig55022_RC ASRGL1 0.3155NM_138619 GGA3 0.3155ENST00000281234 FLJ25621 0.3154AF086207 AF086207 0.3153NM_001361 DHODH 0.3153NM_000664 ACACA 0.3153NM_005736 ACTR1A 0.3152

NM_018911 PCDHA8 0.3152NM_001246 ENTPD2 0.3151NM_016169 SUFU 0.3151Contig24098_RC 0.3151NM_130768 GASZ 0.3151NM_001934 DLX4 0.315NM_000901 NR3C2 0.315NM_030931 DEFB126 0.315NM_032874 FLJ10101 0.3149NM_017626 DNAJB12 0.3148AB076400 FAT3 0.3148NM_004672 MAP3K6 0.3148NM_003710 SPINT1 0.3148NM_004224 GPR50 0.3147NM_177444 PPFIBP1 0.3147NM_030657 LIM2 0.3146NM_018643 TREM1 0.3146Contig34129_RC 0.3145BC004544 MGC13010 0.3145ENST00000280915 ENST000002809 0.3145NM_002067 GNA11 0.3144Contig13815_RC DAPK1 0.3144NM_080618 CTCFL 0.3144NM_174918 LOC199675 0.3144NM_004452 ESRRB 0.3144NM_000399 EGR2 0.3143NM_002429 MMP19 0.3143NM_006246 PPP2R5E 0.3143Contig52222_RC 0.3142NM_014213 HOXD9 0.3142NM_000845 GRM8 0.3142NM_012288 TRAM2 0.3141NM_033005 NALP1 0.3141NM_020526 EPHA8 0.3141Contig461_RC KIAA1836 0.314NM_004785 SLC9A3R2 0.314NM_019555 ARHGEF3 0.314NM_021823 MDS018 0.314Contig25703_RC 0.3139ENST00000229824 ENST0000022982 0.3139NM_017623 CNNM3 0.3138NM_176871 PDLIM2 0.3138NM_003273 TM7SF2 0.3137NM_021006 CCL3L1 0.3137NM_003420 ZNF35 0.3136NM_020172 SPPL2B 0.3134AF131756 SLC1A2 0.3133NM_014909 KIAA1036 0.3133Contig31495 TRIP11 0.3133NM_030783 PTDSS2 0.3133NM_006485 FBLN1 0.3133

NM_004096 EIF4EBP2 0.3133ENST00000267491 C14orf35 0.3132NM_016148 SHANK1 0.3132NM_032364 DNAJ 0.3132NM_180991 SLCO4C1 0.3132NM_002855 PVRL1 0.3132NM_032407 PCDHGC5 0.3132NM_003930 SCAP2 0.3132NM_016151 PSK 0.3132NM_006511 RSC1A1 0.3132NM_004920 AATK 0.3131NM_016056 CGI-119 0.3131NM_022489 FLJ22056 0.3131NM_021197 WFDC1 0.3131AF009314 SEMA5A 0.313NM_000339 SLC12A3 0.313NM_005386 NNAT 0.313NM_004561 OVOL1 0.313NM_004854 CHST10 0.313NM_004519 KCNQ3 0.3129NM_014900 KIAA0977 0.3129NM_003896 SIAT9 0.3129NM_181620 KRTAP22-1 0.3129NM_153619 SEMA6D 0.3128Contig33987 KIAA1371 0.3126AJ406938 KRTAP4-6 0.3126ENST00000300779 ENST000003007 0.3126NM_020642 C11orf17 0.3126NM_015507 EGFL6 0.3125NM_139174 LOC161931 0.3125Contig49943_RC CLCN5 0.3124NM_001840 CNR1 0.3124NM_144991 C21orf29 0.3122NM_033196 LOC91120 0.3121NM_020144 PAPOLB 0.3121NM_015848 HUMCYT2A 0.3121Contig27444_RC 0.312NM_144604 LOC124245 0.3118AK000757 SORT1 0.3117NM_006663 RAI 0.3117NM_024077 SBP2 0.3117AL049357 AL049357 0.3117NM_020749 ATIP1 0.3117NM_001748 CAPN2 0.3117NM_018470 HT009 0.3117NM_005429 VEGFC 0.3115NM_018952 HOXB6 0.3115NM_014412 SIP 0.3115NM_144595 FLJ30046 0.3115ENST00000228567 ENST0000022856 0.3114NM_002147 HOXB5 0.3113

NM_031857 PCDHA9 0.3113NM_152594 SPRED1 0.3113Contig44325_RC 0.3111AK055153 AK055153 0.3111NM_001900 CST5 0.311ENST00000300069 ENST0000030006 0.311NM_015070 KIAA0853 0.311NM_033664 CDH11 0.311NM_000302 PLOD 0.311NM_005633 SOS1 0.3109NM_004213 SLC28A1 0.3109NM_033006 NALP1 0.3109NM_004779 CNOT8 0.3108NM_001221 CAMK2D 0.3108NM_006654 FRS2 0.3107NM_000866 HTR1F 0.3107NM_016190 C1orf10 0.3106Contig25619_RC HSPC043 0.3106Contig35138_RC 0.3105NM_019102 HOXA5 0.3105NM_017766 FLJ20321 0.3105NM_013423 ARHGAP6 0.3104NM_005856 RAMP3 0.3104Contig30775_RC 0.3104NM_152351 FLJ25217 0.3104NM_023037 13CDNA73 0.3103NM_180990 LGICZ 0.3103NM_014081 CG018 0.3103NM_152566 TDH 0.3102NM_016848 SHC3 0.3101Contig21904 IGSF2 0.3101NM_153257 GIOT-1 0.3101NM_172163 KCNQ4 0.3101Contig33235_RC 0.31Contig29721_RC 0.31NM_002585 PBX1 0.31NM_015396 HSPC056 0.31NM_012482 ZNF281 0.3099NM_001917 DAO 0.3099NM_015981 CAMK2A 0.3099Contig54206_RC TNFRSF10D 0.3098NM_172345 SPAG9 0.3098NM_032019 HDAC10 0.3098NM_024087 ASB9 0.3098NM_173802 MGC50559 0.3098NM_032793 FLJ14490 0.3097NM_014862 ARNT2 0.3096NM_006730 DNASE1L1 0.3096NM_032873 KIAA1959 0.3096NM_002359 MAFG 0.3096NM_021603 FXYD2 0.3095

Contig56036_RC CD47 0.3094NM_000840 GRM3 0.3094NM_000054 AVPR2 0.3094NM_148897 SDR-O 0.3094Contig23859_RC 0.3093AB020684 KIAA0877 0.3093NM_000875 IGF1R 0.3093NM_178343 APRG1 0.3093NM_033310 KCNK4 0.3092Contig22251_RC 0.3091Contig13568_RC STK35 0.3091NM_178351 LEP3 0.3091AB014557 KIAA0657 0.309NM_019841 TRPV5 0.309NM_006937 SMT3H2 0.309AK057452 ATP8B3 0.3089Contig45792_RC 0.3088NM_000922 PDE3B 0.3088NM_004225 MFHAS1 0.3087NM_004357 CD151 0.3086NM_033668 ITGB1 0.3086NM_000319 PXR1 0.3085NM_013272 SLC21A11 0.3084Contig51018_RC 0.3084NM_006868 RAB31 0.3083Contig1080_RC PSCD3 0.3083AL713630 AL713630 0.3083NM_001629 ALOX5AP 0.3082NM_024781 FLJ23594 0.3082Contig29277_RC 0.3081Contig48877_RC 0.308Contig33859_RC 0.308NM_032959 POLR2J2 0.308Contig54576_RC FLJ11896 0.3079ENST00000280230 ENST0000028023 0.3079Contig5384_RC 0.3078NM_024733 FLJ14345 0.3078NM_004215 EBAG9 0.3078AK021616 AK021616 0.3076NM_000483 APOC2 0.3076NM_031888 PMCHL2 0.3076NM_005401 PTPN14 0.3076NM_002169 IFNA5 0.3075NM_145268 LOC136263 0.3075NM_015066 TRIM35 0.3075NM_019848 SLC10A3 0.3074AL137406 TIMM9 0.3074NM_025230 WDR23 0.3074NM_014762 DHCR24 0.3074NM_052876 BTBD14B 0.3073NM_014281 SIAHBP1 0.3072

Contig57359_RC LOC51159 0.3071NM_144609 FLJ31795 0.3071NM_012344 NTSR2 0.3071NM_004187 SMCX 0.3071NM_004297 GNA14 0.307NM_002630 PGC 0.307NM_004258 IGSF2 0.307NM_005191 CD80 0.307NM_004670 PAPSS2 0.3069Contig34605_RC 0.3067NM_020465 NDRG4 0.3067NM_000307 POU3F4 0.3067NM_015024 XPO7 0.3067NM_020672 S100A14 0.3066Contig23803_RC MLLT4 0.3066NM_144671 FLJ32356 0.3065NM_175767 IL6ST 0.3065NM_018909 PCDHA6 0.3065Contig25969_RC 0.3064NM_022102 C6orf79 0.3064NM_080652 MGC15397 0.3064AK025156 AK025156 0.3063NM_001914 CYB5 0.3063NM_016545 IER5 0.3062NM_001646 APOC4 0.3062NM_000634 IL8RA 0.3062NM_000590 IL9 0.3062NM_015443 DKFZP727C091 0.3062NM_173657 FLJ31139 0.3061NM_012410 PSK-1 0.306AK055661 AK055661 0.3059Y19237 SYT7 0.3059NM_002177 IFNW1 0.3059NM_001696 ATP6V1E1 0.3058AK025344 AK025344 0.3057NM_025205 EG1 0.3057NM_000720 CACNA1D 0.3057NM_002316 LMX1B 0.3057NM_033025 7h3 0.3056NM_021160 BAT5 0.3056NM_014683 ULK2 0.3055AL080095 0.3055NM_022790 MMP19 0.3055NM_005143 HP 0.3054AL137519 DNCI2 0.3053Contig33654_RC 0.3053NM_006141 DNCLI2 0.3052NM_020314 MGC16824 0.3052NM_016569 TBX3 0.3052AK024580 NRP1 0.3052AK022759 USP42 0.3052

NM_014702 KIAA0408 0.3052NM_130760 MADCAM1 0.3052Contig41377_RC 0.3051NM_002050 GATA2 0.3051Contig7857_RC 0.3051NM_138795 LOC127829 0.3051NM_017629 EIF2C4 0.305NM_018904 PCDHA13 0.305NM_015669 PCDHB5 0.305NM_003505 FZD1 0.3049NM_020061 OPN1LW 0.3049NM_018489 ASH1L 0.3049NM_153267 MAMDC2 0.3049NM_139274 ACAS2 0.3049Contig32439_RC CDIPT 0.3048Contig31613_RC 0.3048NM_024108 MGC2650 0.3048NM_147156 MOB 0.3048NM_033158 HYAL2 0.3047AK000933 CHML 0.3046NM_018239 FLJ10751 0.3045NM_005181 CA3 0.3045NM_012421 RLF 0.3045NM_020204 LHX9 0.3044NM_018295 FLJ11000 0.3043NM_177435 PPARD 0.3043NM_020217 SPTBN1 0.3042NM_014779 KIAA0669 0.3042NM_152679 SLC10A4 0.3042NM_032202 FLJ21404 0.3041NM_000862 HSD3B1 0.3041AL117461 MGC21416 0.304NM_002651 PIK4CB 0.304Contig30029_RC LOC51233 0.3039AL157476 BTBD9 0.3039AL133633 LOC91181 0.3039U00948 CEBPD 0.3039NM_002763 PROX1 0.3039AB020672 MYR8 0.3038NM_003026 SH3GL2 0.3038Contig57662_RC STARD4 0.3038Contig16476_RC 0.3038NM_002410 MGAT5 0.3038NM_004155 SERPINB9 0.3038NM_017847 C1orf27 0.3038NM_003966 SEMA5A 0.3037Contig34779_RC SH3KBP1 0.3036Contig47835_RC KIAA0346 0.3036Contig50939_RC MAP4K5 0.3036NM_005671 D8S2298E 0.3036NM_000776 CYP3A4 0.3035

NM_013382 POMT2 0.3035NM_145277 LOC148738 0.3035NM_004455 EXTL1 0.3034Contig24609_RC 0.3034ENST00000300759 ENST0000030075 0.3034NM_020533 MCOLN1 0.3034NM_024814 CBLL1 0.3034NM_002346 LY6E 0.3034NM_145639 APOL3 0.3034NM_018936 PCDHB2 0.3034AK000787 0.3033NM_138372 LOC91661 0.3033NM_181784 SPRED2 0.3033NM_006471 MRCL3 0.3032U00962 U00962 0.3032NM_182558 FLJ33810 0.3032NM_173831 LOC286075 0.3032NM_145248 LOC122258 0.3031NM_032673 NSPC1 0.3031NM_019554 S100A4 0.3029NM_153711 C6orf188 0.3029NM_175871 FLJ35119 0.3029Contig47416_RC 0.3028NM_005416 SPRR3 0.3028NM_145640 APOL3 0.3028Contig51338_RC HSPC019 0.3027ENST00000297077 ASSP2 0.3027NM_178170 NEK8 0.3027NM_024494 WNT2B 0.3027NM_173664 FLJ39249 0.3027NM_014554 SENP1 0.3026NM_016028 CGI-85 0.3026NM_005037 PPARG 0.3026NM_015649 IRF2BP1 0.3026AK056651 PURB 0.3025NM_170724 PKHD1 0.3025NM_003607 CDC42BPA 0.3024NM_016231 NLK 0.3024NM_016178 OAZ3 0.3024NM_138351 FLJ21941 0.3024NM_020957 PCDHB16 0.3024NM_014601 EHD2 0.3023Contig49891_RC HARC 0.3023NM_178568 NGRH2 0.3023Contig18326_RC 0.3022Contig1018_RC LOC56932 0.3022NM_016639 TNFRSF12A 0.3022Contig30665_RC 0.3021NM_005587 MEF2A 0.302NM_002445 MSR1 0.302NM_020444 KIAA1191 0.302

RSE_00000626103 ODZ2 0.3019NM_181788 LOC341567 0.3019Contig5636_RC 0.3018NM_002217 ITIH3 0.3018NM_133168 OSCAR 0.3018NM_032234 FLJ23059 0.3018AK023547 FLJ21941 0.3017NM_138572 TBN 0.3017Contig6333_RC 0.3016NM_018027 FLJ10210 0.3016NM_002804 PSMC3 0.3015NM_005276 GPD1 0.3015NM_053056 CCND1 0.3015NM_138454 LOC115861 0.3014NM_002931 RING1 0.3014NM_005972 PPYR1 0.3013AF119865 SLCO4C1 0.3013NM_018074 FLJ10374 0.3013NM_007367 RALY 0.3013NM_023931 MGC2474 0.3013NM_017761 PNRC2 0.3012Contig54130_RC DKFZp762G199 0.3012NM_015480 PVRL3 0.3012NM_000709 BCKDHA 0.3012NM_000550 TYRP1 0.3011NM_003447 ZNF165 0.3011NM_033621 CDC2L2 0.3011NM_016040 CGI-100 0.301NM_031460 KCNK17 0.301ENST00000299234 ENST0000029923 0.3009AF258564 VAV2 0.3009NM_181430 ILF1 0.3009AL110157 DUSP7 0.3008NM_013319 TERE1 0.3008NM_000753 PDE3B 0.3007NM_018233 FLJ10826 0.3007NM_181333 ARHGAP8 0.3006NM_013942 PAX3 0.3006Contig23352_RC 0.3005NM_024957 MYO15B 0.3004NM_052948 SNX26 0.3002NM_021908 ST7 0.3001NM_130466 UBE3B 0.3NM_147223 NCOA1 0.3NM_006883 SHOX 0.3NM_001999 FBN2 -0.3AB032953 ODZ2 -0.3002NM_025137 FLJ21439 -0.3002NM_016087 WNT16 -0.3002NM_004888 ATP6V1G1 -0.3002NM_001353 AKR1C1 -0.3004

NM_173481 LOC126353 -0.3005NM_024943 FLJ23235 -0.3005Z70255 Z70255 -0.3006NM_017832 FLJ20457 -0.3006Contig28179_RC -0.3007NM_001606 ABCA2 -0.3007NM_003236 TGFA -0.3009NM_015894 STMN3 -0.3009NM_024748 FLJ11539 -0.3011Contig16262_RC -0.3012NM_030578 MGC4093 -0.3012NM_024306 FAXDC1 -0.3013NM_001928 DF -0.3014Contig38320_RC -0.3014NM_022366 TFB2M -0.3014AK024870 DYRK2 -0.3014NM_148174 OAZIN -0.3014NM_031917 ANGPTL6 -0.3014Contig453_RC C14orf78 -0.3017NM_005902 MADH3 -0.3017AF211972 LENG7 -0.3018NM_006233 POLR2I -0.3018NM_006042 HS3ST3A1 -0.3018NM_006694 JTB -0.3022AK024156 AK024156 -0.3022 4AF055029 -0.3023NM_020639 ANKRD3 -0.3023NM_020655 JPH3 -0.3023AF070587 KIAA1509 -0.3024NM_002315 LMO1 -0.3024NM_005159 ACTC -0.3025NM_005470 SSH3BP1 -0.3026Contig11097_RC -0.3027NM_181652 PRDX5 -0.3027NM_002822 PTK9 -0.3028NM_006066 AKR1A1 -0.3028NM_003357 SCGB1A1 -0.3029NM_004115 FGF14 -0.303NM_016310 POLR3K -0.3031NM_138720 HIST1H2BD -0.3031NM_006847 LILRB4 -0.3032AK025953 MYLK -0.3033NM_025208 SCDGF-B -0.3033NM_005132 REC8L1 -0.3034NM_133636 HEL308 -0.3034NM_013249 ZNF214 -0.3036AF218942 FMN2 -0.3037NM_001248 ENTPD3 -0.3037NM_014679 KIAA0092 -0.3037NM_000993 RPL31 -0.3038NM_145271 LOC146542 -0.3038

e

8

NM_022487 DCLRE1C -0.304S80864 cytochrome c-lik -0.3041NM_012446 SSBP2 -0.3041RSE_00000212796 PIGPC1 -0.3041U90916 SORL1 -0.3042RSE_00000915850 RSE_0000091585 -0.3043NM_025155 FLJ11848 -0.3043AB011099 KIAA0527 -0.3044NM_018103 LRRC5 -0.3044NM_032814 FLJ14627 -0.3045NM_025132 PWDMP -0.3048NM_000655 SELL -0.3049Contig36093_RC -0.3053NM_030979 PABPC3 -0.3053NM_002422 MMP3 -0.3054NM_144717 MGC34923 -0.3054Contig19384_RC -0.3055NM_004812 AKR1B10 -0.3056NM_178452 NM_178452 -0.3061NM_006280 SSR4 -0.3062NM_138461 LOC116211 -0.3063NM_152268 DKFZp727A071 -0.3063NM_003134 SRP14 -0.3063NM_025005 FLJ13315 -0.3063Contig46086_RC -0.3064Contig47683_RC LMLN -0.3066Contig46859_RC SYNPO2 -0.3067NM_002470 MYH3 -0.3071NM_017998 C9orf40 -0.3072NM_058187 C21orf63 -0.3072NM_017773 LAX -0.3072Contig40920 AK3 -0.3073NM_004310 ARHH -0.3074AK055725 MEG3 -0.3075NM_016575 TU12B1-TY -0.3075AF086534 AF086534 -0.3076NM_016280 CES1 -0.3077NM_002129 HMGB2 -0.3077AL359211 AL359211 -0.3078NM_024723 FLJ23471 -0.3078NM_138699 LOC93622 -0.3078AL122107 DKFZp761O201 -0.3079AB020626 KIAA0819 -0.3081NM_017646 IPT -0.3081NM_170726 ALDH4A1 -0.3081NM_006851 GLIPR1 -0.3082NM_031310 PLVAP -0.3085NM_005153 USP10 -0.3085X63945 X63945 -0.3087NM_006086 TUBB4 -0.309Contig31833_RC -0.309

G

NM_003081 SNAP25 -0.309NM_138430 ADPRHL1 -0.309NM_017671 C20orf42 -0.309Contig16786_RC -0.3092NM_014262 LEPREL2 -0.3092NM_177925 H2AFJ -0.3093AL080199 ELOVL2 -0.3094RSE_00000600673 RSE_0000060067 -0.3094NM_004560 ROR2 -0.3094NM_178865 TDE2L -0.3094NM_014965 OIP106 -0.3094NM_172213 CD8B1 -0.3094AL096749 -0.3095NM_181780 BTLA -0.3098NM_014364 GAPDS -0.3099Contig42547 FLJ43842 -0.3099ENST00000245227 RUVBL2,LHB,C -0.3099NM_005608 PTPRCAP -0.31NM_033642 FGF13 -0.31NM_017982 FLJ10052 -0.3101AB028947 KIAA1024 -0.3101Contig32641_RC -0.3102NM_030774 OR51E2 -0.3102NM_004526 MCM2 -0.3102NM_022344 NJMU-R1 -0.3102Contig54295_RC -0.3104NM_002688 PNUTL1 -0.3104X81747 X81747 -0.3106NM_052938 FCRH1 -0.3106NM_024793 KIAA0643 -0.3107NM_001786 CDC2 -0.3108NM_007364 P24B -0.3108NM_018982 DJ167A19.1 -0.3108Contig40743_RC -0.3109NM_024662 FLJ10774 -0.3109NM_017983 FLJ10055 -0.3111NM_032642 WNT5B -0.3111NM_002764 PRPS1 -0.3111NM_024773 FLJ13798 -0.3113AK001906 AK001906 -0.3114Contig23975_RC -0.3115Contig46178_RC -0.3116NM_006875 PIM2 -0.3116NM_000177 GSN -0.3116NM_006682 FGL2 -0.3118NM_024792 CT120 -0.3118NM_014934 DZIP1 -0.3121NM_024649 BBS1 -0.3121NM_030968 C1QTNF1 -0.3121Contig9514_RC -0.3124AK026893 AK026893 -0.3125

0

NM_014264 STK18 -0.3126Contig48076_RC -0.3126Contig34080_RC -0.3127AF086094 AF086094 -0.3127NM_002666 PLIN -0.3128NM_020123 SMBP -0.3128NM_013239 PR48 -0.3129NM_080430 SELM -0.3129NM_018939 PCDHB6 -0.313NM_153610 CMYA5 -0.313Contig36638_RC HNRPD -0.3131M12025 IGLV@ -0.3132NM_172177 MRPL42 -0.3132Contig56093_RC -0.3134Contig46468 LOC123722 -0.3134NM_014707 HDAC9 -0.3134NM_007074 CORO1A -0.3134NM_145258 MGC22773 -0.3134NM_016157 TRO -0.3134NM_153685 DKFZp547D221 -0.3135NM_016350 NIN -0.3135NM_002574 PRDX1 -0.3136AJ132086 DNAH6 -0.3136NM_000572 IL10 -0.3136NM_006366 CAP2 -0.3137NM_014388 MGC29875 -0.3137NM_004538 NAP1L3 -0.3141ENST00000078131 ENST0000007813 -0.3142AK054755 AK054755 -0.3144Contig35884_RC -0.3147NM_001734 C1S -0.3147NM_030934 C1orf25 -0.3147NM_152275 FLJ13946 -0.3148NM_001006 RPS3A -0.3149NM_000057 BLM -0.315NM_001733 C1R -0.3151NM_007215 POLG2 -0.3151NM_025030 FLJ20972 -0.3151NM_003753 EIF3S7 -0.3151NM_000925 PDHB -0.3152AK055386 AK055386 -0.3152NM_144994 DARP -0.3152Contig53962_RC RARS -0.3153NM_001179 ART3 -0.3155NM_030930 UNC93B1 -0.3155NM_033309 MGC4655 -0.3156NM_001901 CTGF -0.3157NM_014677 RIMS2 -0.3158NM_022371 TOR3A -0.316AB028999 KIAA1076 -0.3161NM_032330 CAPNS2 -0.3162

NM_015170 SULF1 -0.3163NM_032361 THOC3 -0.3164NM_001354 AKR1C2 -0.3166NM_173490 LOC134285 -0.3166NM_003864 SAP30 -0.3169NM_080667 MGC15407 -0.3169NM_005145 GNG7 -0.3169NM_012152 EDG7 -0.3169Contig41905_RC -0.317NM_012419 RGS17 -0.3171NM_000847 GSTA3 -0.3171Contig16453_RC CAPN13 -0.3172NM_004415 DSP -0.3173NM_021831 FLJ21839 -0.3174Contig51373_RC KIAA1688 -0.3175AB007883 KIAA0423 -0.3176Contig51558_RC ARGBP2 -0.3176NM_145063 C6orf130 -0.3176AF086158 AF086158 -0.3177NM_024035 MGC3113 -0.3177NM_052947 HAK -0.3178NM_006827 TMP21 -0.3181NM_182552 MGC43690 -0.3182NM_003576 STK24 -0.3185ENST00000295072 ENST000002950 -0.3186NM_024507 KREMEN2 -0.3186NM_005527 HSPA1L -0.3187Contig16366_RC -0.3188NM_016025 DREV1 -0.3189NM_020128 MDM1 -0.3189NM_007054 KIF3A -0.319NM_000050 ASS -0.3191NM_174890 ANUBL1 -0.3193Contig47629_RC -0.3196NM_005233 EPHA3 -0.3196Contig37593_RC URB -0.3197NM_004931 CD8B1 -0.3199NM_000903 NQO1 -0.32NM_006431 CCT2 -0.32NM_003254 TIMP1 -0.3203NM_145739 OSBPL6 -0.3204AK055841 ATP6V0A4 -0.3205NM_178432 CCRK -0.3205NM_018456 EAF2 -0.3207NM_080913 ASGR2 -0.3207NM_003366 UQCRC2 -0.3208NM_005689 ABCB6 -0.3208NM_002699 POU3F1 -0.3208RSE_00000581377 NPM1 -0.3209NM_058193 TRIM29 -0.3209NM_014274 TRPV6 -0.321

X57819 IGHG3 -0.3211NM_001100 ACTA1 -0.3212NM_024826 FLJ21159 -0.3212NM_145006 MGC26847 -0.3212NM_145312 LOC220992 -0.3213NM_012205 HAAO -0.3214NM_033655 CASPR3 -0.3215Contig44604_RC ADCY5 -0.3216Contig41698_RC DJ667H12.2 -0.3216Contig14671_RC -0.3219NM_130843 PTPRN2 -0.3219NM_006422 AKAP3 -0.322AK024106 AK024106 -0.322NM_033199 SRP -0.3222Contig14385_RC -0.3223AB051466 KIAA1679 -0.3223NM_031942 CDCA7 -0.3224Contig14796_RC SRrp35 -0.3225NM_001792 CDH2 -0.3226NM_005249 FOXG1B -0.3227NM_002638 PI3 -0.3227Contig13862_RC -0.3228Contig53969_RC -0.3229NM_018134 FLJ10547 -0.3229NM_152598 FLJ35757 -0.323Contig39909_RC -0.3231Contig54661_RC HOOK1 -0.3231Contig39100_RC -0.3232NM_001263 CDS1 -0.3233NM_145725 TRAF3 -0.3233NM_016004 C20orf9 -0.3235NM_001927 DES -0.3236NM_138788 LOC120224 -0.3236NM_178129 P2RY8 -0.3238NM_001445 FABP6 -0.3241NM_152571 FLJ36779 -0.3242AF038201 -0.3243Contig47781_RC -0.3244NM_012144 DNAI1 -0.3244NM_002614 PDZK1 -0.3245NM_178470 KIAA1892L -0.3245NM_145260 OSR1 -0.3246NM_002431 MNAT1 -0.3247NM_006765 N33 -0.3248Contig28123_RC -0.3248AK056037 FUBP3 -0.3249NM_014599 MAGED2 -0.3249NM_014014 NM_014014 -0.325Contig15441_RC -0.3251Contig9643_RC MYCD -0.3252NM_001911 CTSG -0.3252

NM_005588 MEP1A -0.3253Contig20989 KIAA2022 -0.3253NM_013367 ANAPC4 -0.3253NM_021181 SLAMF7 -0.3254NM_006457 LIM -0.3258NM_016570 PTX1 -0.3259NM_006232 POLR2H -0.3261NM_001652 AQP6 -0.3261NM_017573 PCSK4 -0.3261ENST00000298818 HBLD1 -0.3262AK054570 NCAM1 -0.3262NM_032661 MGC5139 -0.3263NM_003068 SNAI2 -0.3263NM_031308 EPPK1 -0.3264NM_006406 PRDX4 -0.3266NM_002512 NME2 -0.3266NM_005014 OMD -0.3268NM_014041 SPC12 -0.327ENST00000300811 MGC51082 -0.327NM_175738 RAB37 -0.327NM_002237 KCNG1 -0.3271NM_000972 RPL7A -0.3272NM_005923 MAP3K5 -0.3273Contig55734_RC LOC63929 -0.3274NM_007258 VPS45A -0.3275NM_002507 NGFR -0.3277Contig52433_RC -0.3278Contig43688_RC CDS1 -0.3278NM_032848 FLJ14827 -0.3278NM_153202 ADAM33 -0.3278NM_177990 PAK7 -0.3278NM_003360 UGT8 -0.3279NM_025087 FLJ21511 -0.3279NM_000618 IGF1 -0.3279Contig37708_RC SLC35F1 -0.3281Contig38724_RC -0.3283NM_152323 SPIC -0.3283NM_176891 IFNT1 -0.3283ENST00000300244 ENST0000030024 -0.3284NM_138784 LOC116123 -0.3285NM_000826 GRIA2 -0.3286D31887 SLC39A14 -0.3286NM_017843 BCAS4 -0.3286Contig20793_RC -0.3287NM_000803 FOLR2 -0.3287NM_016459 PACAP -0.3289NM_030956 TLR10 -0.3293NM_004245 TGM5 -0.3295NM_021827 FLJ23514 -0.3295NM_016627 LOC51321 -0.3296NM_003518 HIST1H2BG -0.3296

2

NM_178555 FLJ25770 -0.3296NM_020739 CPR8 -0.3296NM_017939 FLJ20718 -0.3297NM_003610 RAE1 -0.3299NM_000702 ATP1A2 -0.3303Contig24780_RC -0.3303NM_003121 SPIB -0.3304NM_003918 GYG2 -0.3307NM_001369 DNAH5 -0.3307NM_178274 URB -0.3307NM_145756 ZNF396 -0.3308NM_032574 LOC84661 -0.3309NM_016086 MK-STYX -0.3311NM_024007 EBF -0.3311NM_176791 C20orf65 -0.3312NM_002591 PCK1 -0.3312NM_019064 SDK2 -0.3312NM_138483 LOC151568 -0.3313NM_005887 DLEU1 -0.3313NM_014161 MRPL18 -0.3315NM_004279 PMPCB -0.3315NM_003063 SLN -0.3316NM_005235 ERBB4 -0.3318NM_003086 SNAPC4 -0.3318Contig46564_RC SIAT8D -0.3319Contig40158_RC ITGA2 -0.332NM_004523 KIF11 -0.332NM_002893 RBBP7 -0.3323NM_014809 KIAA0319 -0.3323NM_021203 APMCF1 -0.3324NM_025228 T3JAM -0.3324NM_152392 AHSA2 -0.3324ENST00000287529 ENST0000028752 -0.3325NM_182746 MCM4 -0.3327NM_022147 IFRG28 -0.3327NM_021810 CDH26 -0.3328NM_024726 FLJ22527 -0.3329NM_145341 PDCD4 -0.333NM_017938 FLJ20716 -0.3331NM_005822 DSCR1L1 -0.3332NM_032117 GAJ -0.3332NM_173576 C10orf48 -0.3334NM_019892 INPP5E -0.3336NM_030796 DKFZP564K082 -0.3336NM_003701 TNFSF11 -0.3336NM_152328 ADSSL1 -0.3337Contig31101_RC XPO1 -0.3338NM_006665 HPSE -0.3338ENST00000296941 ENST0000029694 -0.334Contig57239_RC KIAA0114 -0.3341NM_032254 DKFZp434F142 -0.3343

NM_002095 GTF2E2 -0.3348Contig45418_RC FLJ35283 -0.3348AK025062 SLC12A2 -0.3348NM_006461 SPAG5 -0.3351NM_002703 PPAT -0.3351NM_018464 MDS029 -0.3352NM_033559 CAPN13 -0.3352RSE_00000623559 RSE_0000062355 -0.3354Contig40000_RC KIAA1674 -0.3354NM_002621 PFC -0.3355NM_000764 CYP2A7 -0.3356NM_005987 SPRR1A -0.3357NM_000732 CD3D -0.3358NM_012094 PRDX5 -0.3358NM_182598 FLJ36980 -0.3358AK002088 -0.3359NM_002712 PPP1R7 -0.336NM_018672 ABCA5 -0.3362NM_006227 PLTP -0.3364NM_005581 LU -0.3364AB018317 KIAA0774 -0.3365Contig15728_RC -0.3365AL049969 LIM -0.3367NM_001102 ACTN1 -0.3369NM_025103 CCDC2 -0.337NM_003615 SLC4A7 -0.337NM_182497 FLJ36600 -0.3371Contig40018_RC SR140 -0.3372NM_020383 XPNPEP1 -0.3372NM_138458 LOC116143 -0.3373NM_173509 MGC16664 -0.3373NM_178840 MGC24047 -0.3375NM_002887 RARS -0.3377AF103458 IGKC -0.3379NM_014383 TZFP -0.338NM_001426 EN1 -0.338NM_006429 CCT7 -0.3381NM_001333 CTSL2 -0.3383NM_000095 COMP -0.3383NM_003114 SPAG1 -0.3383NM_022843 PCDH20 -0.3384NM_178122 LOC90529 -0.3384NM_020632 ATP6V0A4 -0.3385AB058769 FNDC1 -0.3385NM_020347 LZTFL1 -0.3386ENST00000224199 KIAA0534 -0.3389NM_173341 PHF7 -0.3389NM_031455 DKFZP761F241 -0.3389NM_002677 PMP2 -0.339Contig32649_RC -0.3391ENST00000267799 ENST0000026779 -0.3391

M87789 IGHG3 -0.3392NM_006685 PROL3 -0.3397NM_014622 LOH11CR2A -0.3397Contig48043_RC ABCC5 -0.3398ENST00000295853 ENST0000029585 -0.3399NM_006139 CD28 -0.34NM_002492 NDUFB5 -0.34NM_032910 C21orf119 -0.3403ENST00000297930 ENST0000029793 -0.3403NM_000130 F5 -0.3404NM_004409 DMPK -0.3404NM_014920 ICK -0.3404NM_012452 TNFRSF13B -0.3404NM_000275 OCA2 -0.3404Contig51441_RC -0.3405NM_173512 FLJ39822 -0.3406NM_178557 FLJ37478 -0.3407NM_001553 IGFBP7 -0.3408NM_024604 FLJ21908 -0.3409ENST00000301513 ENST000003015 -0.3411NM_025126 RNF34 -0.3412Contig46075_RC -0.3414NM_002161 IARS -0.3415ENST00000299546 ENST0000029954 -0.3415AF052141 -0.3416NM_004595 SMS -0.3417AF086019 AF086019 -0.3417AK023027 AK023027 -0.3419NM_000526 KRT14 -0.3421NM_054020 CATSPER2 -0.3422NM_176827 NM_176827 -0.3423NM_013417 IARS -0.3424NM_032756 MGC15668 -0.3424Contig48358_RC PLXNC1 -0.3426NM_145298 APOBEC3F -0.3428NM_002474 MYH11 -0.3428NM_002168 IDH2 -0.3431ENST00000286095 ENST0000028609 -0.3433AL157492 C14orf171 -0.3437NM_024823 EPB41L4B -0.3437NM_139166 STARS -0.3437L23563 IGHG3 -0.3438NM_152702 FLJ35283 -0.3438NM_022121 PERP -0.3439NM_005662 VDAC3 -0.3442NM_014051 TMEM14A -0.3443NM_005173 ATP2A3 -0.3443NM_022098 LOC63929 -0.3444NM_173799 FLJ39873 -0.3444NM_014659 KIAA0377 -0.3445Contig48506_RC KIAA1799 -0.3445

NM_032991 CASP3 -0.3448NM_002481 PPP1R12B -0.345Contig39268_RC -0.3452NM_019847 ANKH -0.3454NM_004658 RASAL1 -0.3455NM_002765 PRPS2 -0.3455NM_007366 PLA2R1 -0.3456NM_012132 CLDN8 -0.3457Contig48400_RC ENPP1 -0.3457NM_000792 DIO1 -0.3459NM_001002 RPLP0 -0.346NM_054110 GALNT7 -0.346Contig6654_RC -0.3461Contig53296_RC -0.3461NM_012111 AHSA1 -0.3464NM_000643 AGL -0.3464NM_033254 BOC -0.3465NM_001697 ATP5O -0.3465NM_015557 CHD5 -0.347AB037798 KIAA1377 -0.3472NM_173570 MGC42530 -0.3472NM_005531 IFI16 -0.3473NM_017423 GALNT7 -0.3474NM_000599 IGFBP5 -0.3474NM_004308 ARHGAP1 -0.3474NM_052945 TNFRSF13C -0.3475AF131846 GFPT1 -0.3476NM_009588 LTB -0.3476NM_000053 ATP7B -0.3476NM_032499 HH114 -0.3479NM_001279 CIDEA -0.3483NM_018646 TRPV6 -0.3484NM_018063 HELLS -0.3485Contig16239_RC -0.3486NM_014350 GG2-1 -0.3486NM_000028 AGL -0.3487NM_002520 NPM1 -0.3489Contig42213_RC -0.3489NM_004181 UCHL1 -0.349Contig47326 -0.349NM_001833 CLTA -0.3492NM_001458 FLNC -0.3493NM_182767 NTT73 -0.3493Contig5392_RC -0.3496AL133591 -0.3496NM_001778 CD48 -0.3497NM_033316 MFI2 -0.3504NM_178232 HAPLN3 -0.3506NM_020927 KIAA1576 -0.3507NM_016567 BCCIP -0.3507NM_024837 ATP8B4 -0.3507

ENST00000272822 ENST0000027282 -0.351NM_032932 RAB11-FIP4 -0.351NM_152506 C21orf129 -0.351NM_005578 LPP -0.3511NM_003687 RIL -0.3511NM_014792 KIAA0125 -0.3513NM_004998 MYO1E -0.3513ENST00000285188 ENST0000028518 -0.3513NM_005824 LRRC17 -0.3514NM_005003 NDUFAB1 -0.3515NM_024865 NANOG -0.3515NM_006941 SOX10 -0.3516NM_018446 AD-017 -0.3518NM_004766 COPB2 -0.352NM_001192 TNFRSF17 -0.3521Contig43373_RC -0.3522NM_003202 TCF7 -0.3522NM_133326 ATP6V1G3 -0.3524NM_138379 LOC91937 -0.3525NM_130810 DYX1C1 -0.3525NM_181714 C6orf152 -0.3526NM_004876 ZNF254 -0.3526AF075091 AF075091 -0.3527NM_020443 NAV1 -0.3527NM_001177 ARL1 -0.3527Contig21192 -0.3529NM_001555 IGSF1 -0.3529NM_004747 DLG5 -0.3529NM_173623 FLJ35808 -0.3531Contig37236_RC -0.3534NM_032511 C6orf168 -0.3535NM_014177 HSPC154 -0.3539NM_006798 UGT2A1 -0.354NM_018365 MNS1 -0.3541Contig16902_RC -0.355Contig38980_RC -0.3551Contig46589_RC GFPT1 -0.3553NM_002497 NEK2 -0.3554NM_006398 UBD -0.3555AK024123 AK024123 -0.3556NM_004092 ECHS1 -0.3557NM_020432 PHTF2 -0.3558Contig42161_RC -0.3559ENST00000295471 ENST000002954 -0.3559NM_030764 SPAP1 -0.3563NM_001729 BTC -0.3565NM_176782 MGC27169 -0.3566Contig15795_RC -0.3568NM_004506 HSF2 -0.3568Contig14831_RC -0.3569NM_153377 NM_153377 -0.3569

Contig15031_RC HOXA2 -0.357NM_000518 HBB -0.357NM_005292 GPR18 -0.3571NM_014479 ADAMDEC1 -0.3573NM_025206 FER1L4 -0.3573NM_001216 CA9 -0.3574NM_001203 BMPR1B -0.3574NM_015385 SORBS1 -0.3575NM_025237 SOST -0.3575Contig66615_RC MGC71745 -0.3576NM_147161 THEA -0.3576BC014342 TTC6 -0.3578NM_003880 WISP3 -0.3578NM_004036 ADCY3 -0.3579AK022789 AK022789 -0.358NM_007308 SNCA -0.3581NM_182798 FLJ39155 -0.3581NM_002036 FY -0.3584NM_053277 CLIC6 -0.3584Contig29015_RC -0.3587NM_152357 ZNF440 -0.3587NM_012108 BRDG1 -0.3588NM_016237 ANAPC5 -0.3588Contig23513_RC -0.359NM_005336 HDLBP -0.359AK024886 AK024886 -0.359NM_005653 TFCP2 -0.359ENST00000283696 ENST0000028369 -0.3591NM_024735 MGC15419 -0.3591X72465 X72465 -0.3593Contig27908_RC NPAS3 -0.3596NM_152385 FLJ31438 -0.3597Contig49175_RC DKFZp313A2432 -0.3598NM_015964 CGI-38 -0.3598AK001164 -0.3599Contig9904_RC KIAA1229 -0.3602NM_138732 NRXN2 -0.3608NM_152587 MGC33948 -0.3608Contig6641_RC -0.361NM_000421 KRT10 -0.361NM_003248 THBS4 -0.3611NM_016140 CGI-38 -0.3613NM_000691 ALDH3A1 -0.3614NM_033111 LOC88523 -0.3616NM_057157 CYP26A1 -0.3617AL389942 AL389942 -0.362NM_016594 FKBP11 -0.3623AK056229 AK056229 -0.3624NM_013243 SCG3 -0.3625AL137342 UGT8 -0.363NM_014428 TJP3 -0.363

NM_021121 EEF1B2 -0.3631NM_003797 EED -0.3631NM_005260 GDF9 -0.3633NM_003720 DSCR2 -0.3633ENST00000300016 ENST000003000 -0.3634NM_016339 Link-GEFII -0.3637NM_012267 HSPBP1 -0.3639NM_006638 RNASEP1 -0.364NM_033128 SCIN -0.3643NM_018142 FLJ10569 -0.3644NM_152723 FLJ38159 -0.3644NM_014366 NS -0.3645NM_181361 KCNMB2 -0.3646NM_017861 FLJ20522 -0.3646Contig25994_RC EYA4 -0.3647NM_138408 C6orf51 -0.3651AB014513 LDB3 -0.3654NM_004361 CDH7 -0.3654NM_018176 LGI2 -0.3656NM_018139 C14orf104 -0.3656NM_002153 HSD17B2 -0.3657Contig55487_RC SLC39A10 -0.3659NM_018137 PRMT6 -0.366NM_138738 SPAP1 -0.3662NM_014321 ORC6L -0.3663ENST00000267485 C14orf37 -0.3664NM_025220 ADAM33 -0.3664NM_145014 FLJ32915 -0.3666NM_014141 CNTNAP2 -0.3666NM_000253 MTP -0.3668NM_001838 CCR7 -0.3669NM_021052 HIST1H2AE -0.367NM_024571 C16orf33 -0.367NM_002989 CCL21 -0.3672NM_002341 LTB -0.3674NM_018556 SIRPB2 -0.3675NM_144666 FLJ32752 -0.3675AK023367 FLJ13305 -0.3677NM_139076 FLJ13614 -0.3677NM_012333 MYCBP -0.3681NM_053001 OSR2 -0.3681NM_012119 CCRK -0.3682NM_030919 C20orf129 -0.3682NM_021200 PLEKHB1 -0.3683NM_022055 KCNK12 -0.3683NM_032289 DKFZp761B0514 -0.3683NM_015286 DMN -0.3683NM_174953 ATP2A3 -0.3683NM_003776 MRPL40 -0.3687NM_015994 ATP6V1D -0.3687NM_003090 SNRPA1 -0.3689

NM_005548 KARS -0.369NM_024945 FLJ12888 -0.369NM_033058 RNF29 -0.369NM_005348 HSPCA -0.369NM_015444 RIS1 -0.369NM_030936 RNF32 -0.3692NM_014932 NLGN1 -0.3696Contig38472_RC FLJ21195 -0.3697Contig28909_RC -0.3697NM_003157 NEK4 -0.3697NM_004833 AIM2 -0.3698NM_173812 FLJ32949 -0.3699AK026466 AK026466 -0.37Contig41762 FLJ36032 -0.3701NM_006659 TUBGCP2 -0.3701NM_014675 CROCC -0.3704NM_145004 ADAM32 -0.3706NM_178127 ANGPTL5 -0.3706NM_014332 SMPX -0.3707NM_000884 IMPDH2 -0.3707NM_016619 PLAC8 -0.3708NM_178820 FBXO27 -0.3709NM_003617 RGS5 -0.371NM_152517 FLJ30990 -0.371NM_017662 TRPM6 -0.3712NM_145017 FLJ32771 -0.3714NM_003270 TM4SF6 -0.3714NM_024702 FLJ13841 -0.3716NM_013304 ZDHHC1 -0.3718NM_002514 NOV -0.3718NM_012157 FBXL2 -0.372NM_006274 CCL19 -0.3721NM_017412 FZD3 -0.3722Contig35984_RC MICAL3 -0.3727NM_001584 C11orf8 -0.3729NM_000065 C6 -0.373Contig26077_RC -0.373NM_019118 RP4-622L5 -0.3733NM_003247 THBS2 -0.3735NM_015162 BG1 -0.3737NM_001537 HSBP1 -0.3738NM_000672 ADH6 -0.3742Contig42490_RC -0.3742NM_018968 SNTG2 -0.3743Contig22995_RC -0.3745NM_153191 SLC22A2 -0.3745NM_133639 ARHV -0.3748AK056227 C17orf36 -0.3749NM_030574 STARD5 -0.3749NM_031966 CCNB1 -0.375NM_012189 CABYR -0.3751

AK021801 AK021801 -0.3751Contig34768_RC C21orf59 -0.3752NM_013378 VPREB3 -0.3754Contig24875_RC -0.3755NM_016284 KIAA1007 -0.3755NM_024110 CARD14 -0.3755NM_000646 AGL -0.3756Contig53965_RC HS3ST3B1 -0.3757NM_030674 SLC38A1 -0.376Contig16654_RC T3JAM -0.3761NM_022726 ELOVL4 -0.3763NM_001775 CD38 -0.3767NM_018073 RNF137 -0.3769NM_005039 PRB1 -0.377Contig20361_RC KIAA1755 -0.3771NM_005618 DLL1 -0.3772NM_152403 FLJ39155 -0.3773AF131799 SDK1 -0.3776NM_005038 PPID -0.3777NM_006769 LMO4 -0.3779Contig31800_RC -0.3779NM_025164 KIAA0999 -0.3779NM_024606 DNCH2 -0.3781NM_016080 CGI-150 -0.3782NM_017668 NDE1 -0.3782NM_032902 PPP1R16A -0.3783RSE_00000613195 RSE_0000061319 -0.3784NM_178558 FLJ90430 -0.3785NM_006414 RPP38 -0.3785NM_172165 MSH5 -0.3785NM_000938 POLR2B -0.3787NM_002276 KRT19 -0.3787NM_004166 CCL14 -0.3788NM_002667 PLN -0.3788NM_014452 TNFRSF21 -0.3793NM_000783 CYP26A1 -0.3794NM_004338 C18orf1 -0.3797NM_021601 CD79A -0.3797NM_005030 PLK -0.3797Contig33749_RC RPLP2 -0.3801NM_025069 FLJ14299 -0.3801NM_032738 FREB -0.3802NM_014715 RICS -0.3803ENST00000295298 ENST0000029529 -0.3805NM_134433 RFX2 -0.3805NM_015271 TRIM2 -0.3806Contig29982_RC MGC45780 -0.3807NM_025236 RNF39 -0.3807NM_002644 PIGR -0.3807K03208 PRB2 -0.3811NM_052832 SLC26A7 -0.3812

NM_014010 ASTN2 -0.3818Contig27084_RC -0.382Contig20748_RC -0.3823NM_005876 APEG1 -0.3824NM_001170 AQP7 -0.3825NM_052904 KIAA1900 -0.3825NM_000213 ITGB4 -0.3826NM_001135 AGC1 -0.3827NM_004711 SYNGR1 -0.3828Contig263_RC C14orf168 -0.3828ENST00000239121 FLJ35908 -0.383NM_174912 FLJ31204 -0.383NM_002379 MATN1 -0.3831NM_000149 FUT3 -0.3832NM_144725 FLJ25439 -0.3832ENST00000272799 ENST0000027279 -0.3833NM_030814 C9orf45 -0.3834NM_152597 FLJ35989 -0.3834NM_014205 C11orf5 -0.3837NM_002349 LY75 -0.3837Contig23454_RC -0.3838Contig48910_RC -0.3839AF085900 AF085900 -0.3839NM_014424 HSPB7 -0.3843NM_024753 FLJ11457 -0.3843NM_004631 LRP8 -0.3843NM_003951 SLC25A14 -0.3843Contig39385_RC -0.3844NM_005752 CLECSF1 -0.3844NM_016499 MGC:13379 -0.3844NM_005449 TOSO -0.3845NM_152759 MGC35140 -0.3845NM_018004 FLJ10134 -0.3846NM_172233 EHF -0.3847NM_000624 SERPINA5 -0.3849NM_001395 DUSP9 -0.3852NM_003719 PDE8B -0.3853Contig40997_RC LOC157657 -0.3854NM_052966 C1orf24 -0.3855NM_005657 TP53BP1 -0.3857NM_024728 C7orf10 -0.3857RSE_00000670375 RSE_0000067037 -0.3858Contig53838_RC -0.386NM_014226 RAGE -0.386AF052152 -0.3862NM_002698 POU2F2 -0.3862NM_016599 MYOZ2 -0.3862AB033055 KIAA1229 -0.3863NM_052873 MGC16028 -0.3864ENST00000298009 ENST0000029800 -0.3865NM_001716 BLR1 -0.3865

AF086342 AF086342 -0.3868Contig1998_RC ZNF36 -0.3869AB037820 KIAA1399 -0.3869NM_018234 TSAP6 -0.3871L27560 IGFBP5 -0.3872NM_001047 SRD5A1 -0.3875Contig13874_RC -0.3876NM_015080 NRXN2 -0.3877NM_005164 ABCD2 -0.3881AL157504 -0.3882NM_000667 ADH1A -0.3884NM_024506 MGC10771 -0.3884NM_172097 CATSPER2 -0.3885NM_031938 BCDO2 -0.3886NM_014055 CDV-1 -0.389NM_005804 DDX39 -0.389Contig42354 LDB3 -0.3892Contig24818_RC -0.3893NM_004476 FOLH1 -0.3893NM_012326 MAPRE3 -0.3893NM_002046 GAPD -0.3894Contig45730_RC DJ667H12.2 -0.3894AF085951 MCSC -0.3894Contig13173_RC -0.3895ENST00000295549 LOC375295 -0.3897NM_003866 INPP4B -0.39NM_016175 LOC51149 -0.39NM_152485 FLJ25078 -0.3901Contig39242_RC SDK1 -0.3902NM_021956 GRIK2 -0.3904Contig31398_RC FLJ21062 -0.3906NM_152341 FLJ30002 -0.3908NM_013280 FLRT1 -0.3909NM_144633 KCNH8 -0.391NM_021101 CLDN1 -0.3911Contig22517_RC -0.3912NM_152512 FLJ25421 -0.3914NM_172218 SPAG1 -0.3914NM_016194 GNB5 -0.3917NM_152448 MGC33951 -0.3917NM_178510 ANKK1 -0.3919Contig9369_RC -0.392NM_033549 TRIM41 -0.3922NM_018319 TDP1 -0.3925NM_006099 PIAS3 -0.3933Contig24252_RC RECQL5 -0.3934AK024907 AK024907 -0.3936NM_022097 LOC63928 -0.3937NM_003784 SERPINB7 -0.3938NM_030958 SLC21A15 -0.3938NM_002031 FRK -0.3939

e

NM_002061 GCLM -0.3941NM_000087 CNGA1 -0.3942NM_024788 FLJ21062 -0.3944Contig47919_RC ARHN -0.3946NM_006207 PDGFRL -0.3948NM_022893 BCL11A -0.3948AB051431 KIAA1644 -0.3956Contig46187_RC ADAMTS14 -0.3957AK002039 MRVI1 -0.3966NM_014926 KIAA0848 -0.3967Contig47624_RC OACT1 -0.3967NM_004666 VNN1 -0.3968Contig17350_RC hypothetical prot -0.3968NM_002358 MAD2L1 -0.3974NM_182635 LOC349236 -0.3974NM_001362 DIO3 -0.3978NM_144576 FLJ32452 -0.3978ENST00000295220 ENST0000029522 -0.3979Contig30964_RC FLJ32783 -0.398NM_152647 FLJ32800 -0.3981NM_004435 ENDOG -0.3984NM_024888 FLJ11535 -0.3984NM_002557 OVGP1 -0.3989NM_003803 MYOM1 -0.3991Contig39875 -0.3992NM_003384 VRK1 -0.3994NM_000239 LYZ -0.3994NM_004619 TRAF5 -0.3995Contig21929_RC -0.3997NM_000261 MYOC -0.3998NM_021602 CD79B -0.3998NM_019598 KLK12 -0.3999NM_000852 GSTP1 -0.3999NM_031919 CSDUFD1 -0.3999NM_002576 PAK1 -0.4001NM_013371 IL19 -0.4002Contig32706_RC RICS -0.4002NM_005582 LY64 -0.4002NM_130849 SLC39A4 -0.4002NM_002561 P2RX5 -0.4003NM_181785 LOC283537 -0.4005NM_052958 VEST1 -0.4007NM_152709 C10orf24 -0.4007NM_016629 TNFRSF21 -0.401NM_138617 RHCE -0.4011AL157488 -0.4012NM_014057 OGN -0.4019NM_003914 CCNA1 -0.4019NM_016406 HSPC155 -0.4021Contig52427_RC ITGB8 -0.4021NM_018037 FLJ10244 -0.4021

NM_032256 DKFZp434K2435 -0.4022NM_000443 ABCB4 -0.4024NM_152559 WBSCR27 -0.4024NM_024303 ZNF495 -0.4024Contig19224_RC LMLN -0.4025Contig40208_RC ARNTL2 -0.4025ENST00000259983 C6orf52 -0.4026NM_145243 MPRP-1 -0.4026Contig47865 PRO2893 -0.4027Contig48217_RC MAP6 -0.4028NM_021911 GABRB2 -0.4031NM_005097 LGI1 -0.4032NM_177972 TUB -0.4032NM_001615 ACTG2 -0.4038NM_138967 SCAMP5 -0.4039NM_175873 LOC134548 -0.4039NM_002860 PYCS -0.404Contig46136_RC -0.4042NM_004487 GOLGB1 -0.4042NM_017421 COQ3 -0.4043NM_014284 NCDN -0.4043NM_174959 LOC136306 -0.4043NM_018946 NANS -0.4044NM_080877 SLC34A3 -0.4045NM_033103 RHPN2 -0.4046NM_003986 BBOX1 -0.4047Contig78_RC SLMAP -0.4047NM_002427 MMP13 -0.4048NM_019069 WDR5B -0.4048Contig42760_RC USP2 -0.405NM_000349 STAR -0.405AK026320 PIGR -0.4053NM_012472 TSLRP -0.4054NM_173695 FLJ36601 -0.4054NM_002515 NOVA1 -0.4055NM_032211 LOXL4 -0.4056NM_153707 MGC35182 -0.4059NM_006640 MSF -0.406Contig20562_RC -0.406NM_004910 PITPNM1 -0.406NM_022129 MAWBP -0.4062NM_017691 FLJ20156 -0.4063NM_013330 NME7 -0.4069NM_058173 LOC118430 -0.4069Contig25138_RC FLJ32702 -0.407NM_006152 LRMP -0.4071NM_005906 MAK -0.4071NM_014112 TRPS1 -0.4072NM_005917 MDH1 -0.4073Contig36539_RC -0.4075NM_017699 FLJ20174 -0.4076

NM_144593 RHEBL1 -0.4076NM_014174 THY28 -0.408NM_003289 TPM2 -0.4085NM_144964 FLJ31455 -0.4085NM_014446 ITGB1BP3 -0.4087NM_005832 KCNMB2 -0.4089NM_017806 FLJ20406 -0.4092NM_178507 NS5ATP13TP2 -0.4092Contig31699_RC -0.4093NM_032965 CCL15 -0.4094Contig29949_RC -0.4097NM_025063 FLJ23550 -0.4097NM_175870 LOC90925 -0.41NM_015693 NM_015693 -0.4102Contig27623_RC -0.4103NM_002071 GNAL -0.4105AL157425 SOX2OT -0.4106Contig16161_RC LOC129881 -0.4106NM_013391 DMGDH -0.4107NM_006741 PPP1R1A -0.411NM_025227 BPIL1 -0.411NM_030906 STK33 -0.4111NM_139172 MDAC1 -0.4111NM_152361 FLJ38944 -0.4112NM_031898 TEKT3 -0.4115Contig26144_RC -0.4119NM_024809 FLJ12975 -0.412NM_001888 CRYM -0.4122NM_178034 cPLA2delta -0.4122NM_033014 OGN -0.4127NM_014804 KIAA0753 -0.4127NM_006183 NTS -0.4128AF108756 FGF13 -0.4128RSE_00000674442 RSE_0000067444 -0.413NM_019845 REPRIMO -0.4131ENST00000286557 ENST0000028655 -0.4132NM_173622 FLJ36674 -0.4137Contig26837_RC -0.4138NM_018203 FLJ10748 -0.4139NM_153611 MGC20446 -0.4139NM_003115 UAP1 -0.414NM_145720 TIGD4 -0.4141NM_002247 KCNMA1 -0.4144Contig54847_RC -0.4145NM_020892 DTX2 -0.4145AK056076 FLJ31514 -0.4149NM_017600 DKFZp434M0331 -0.4149NM_138962 MSI2 -0.4151NM_017777 FLJ20345 -0.4153AL117421 SLB -0.4154NM_032029 FKSG87 -0.4154

NM_152394 MGC39662 -0.4154Contig297_RC -0.4155NM_017436 A4GALT -0.4156NM_017950 FLJ20753 -0.4157Contig20953_RC -0.4158NM_018956 C9orf9 -0.4163NM_017696 C6orf61 -0.4163NM_057176 BSND -0.4165NM_006167 NKX3-1 -0.4165NM_007271 STK38 -0.4167NM_017918 FLJ20647 -0.4168ENST00000216471 ENST000002164 -0.4172ENST00000286203 FLJ34497 -0.4173NM_001844 COL2A1 -0.4175NM_000772 CYP2C18 -0.4175NM_002300 LDHB -0.4176NM_138342 LOC89944 -0.4177NM_173193 KCNIP2 -0.4178NM_002947 RPA3 -0.4179Contig6568_RC -0.418AL110240 DKFZP566F084 -0.4181AK001469 GNPNAT1 -0.4181NM_006419 CXCL13 -0.4181NM_005847 SLC23A1 -0.4182AK025378 AK025378 -0.4184Contig51981_RC LGR6 -0.4185Contig58512_RC SFRP2 -0.4185NM_006644 HSPH1 -0.4187NM_138816 C6orf198 -0.4187Contig56583_RC PF20 -0.4192NM_000695 ALDH3B2 -0.4192NM_006519 TCTEL1 -0.4194NM_033625 RPL34 -0.4194NM_173076 ABCA12 -0.4197NM_152932 AD-017 -0.4197NM_025044 BICC1 -0.4198NM_024780 TMC5 -0.4202NM_145644 MRPL35 -0.4202AL110170 AL110170 -0.4203NM_014562 OTX1 -0.4203NM_006430 CCT4 -0.4203NM_145241 WDR31 -0.4205NM_015341 BRRN1 -0.4206NM_018405 HSA272196 -0.4208NM_004403 DFNA5 -0.4209Contig55201_RC -0.4211Contig42395_RC WDR5B -0.4212NM_016240 SCARA3 -0.4217AL359582 AL359582 -0.4224ENST00000294805 ENST0000029480 -0.4225NM_013233 STK39 -0.4226

AK021549 AK021549 -0.4227NM_134428 RFX3 -0.4227NM_015453 DKFZP434F091 -0.4228Contig39732_RC FGF14 -0.4231AK023391 FBXO32 -0.4232NM_130808 CPNE4 -0.4232AJ420416 GCNT1 -0.4233NM_032297 DKFZp761D112 -0.4237NM_005340 HINT1 -0.4237NM_003014 SFRP4 -0.4239NM_023928 AACS -0.4241NM_139177 C17orf26 -0.4241Contig26724_RC PVT1 -0.4242NM_012317 LDOC1 -0.4243NM_000676 ADORA2B -0.4245Contig52937_RC -0.4246NM_006197 PCM1 -0.4249NM_001438 ESRRG -0.425NM_080827 WFDC6 -0.425NM_022725 FANCF -0.4251NM_022754 SFXN1 -0.4253AL122120 DKFZp434A128 -0.4255AB037734 PCDH19 -0.4255NM_004798 KIF3B -0.4255NM_020939 CPNE5 -0.4257NM_017495 RNPC1 -0.426NM_144649 FLJ33069 -0.426NM_006884 SHOX2 -0.4261NM_024921 POF1B -0.4261NM_173677 FLJ40852 -0.4262NM_024677 FLJ14001 -0.4262NM_000343 SLC5A1 -0.4264NM_175853 LOC150759 -0.4266AF301222 NY-REN-41 -0.4267NM_175605 TG737 -0.4267AB037766 KIAA1345 -0.4269NM_145294 KIAA1924 -0.4269ENST00000277146 ENST0000027714 -0.4272NM_145173 DIRAS1 -0.4273Contig48371 MDH1 -0.4274NM_000970 RPL6 -0.4276NM_015412 DKFZP434F2021 -0.4276NM_022659 EBF2 -0.4277Contig51309_RC RAB6B -0.4281NM_006584 CCT6B -0.4282NM_021048 MAGEA10 -0.4285AK025613 STRBP -0.4285AK056063 AK056063 -0.4285NM_080818 GPR80 -0.4285NM_017732 PH-4 -0.4286NM_006249 PRB3 -0.4286

NM_012449 STEAP -0.4287NM_006317 BASP1 -0.429Contig25659_RC GALNT4 -0.4291NM_022144 TNMD -0.4291AK021853 AK021853 -0.4294NM_032107 L3MBTL -0.4295NM_018057 NTT73 -0.4296NM_024764 C14orf161 -0.4296Contig54491_RC DSCR1L2 -0.4299NM_153696 PSMAL/GCP III -0.43NM_138415 LOC112885 -0.4302NM_007029 STMN2 -0.4304NM_130387 ASB14 -0.4306NM_006666 RUVBL2 -0.4306NM_032849 FLJ14834 -0.4307NM_030770 TMPRSS5 -0.4308Contig34348_RC NCAM1 -0.4309AF086431 AF086431 -0.4309NM_006670 TPBG -0.431NM_025125 FLJ13263 -0.4317NM_005441 CHAF1B -0.4317NM_058229 FBXO32 -0.432Contig44877_RC ST6GalII -0.4321NM_017868 TTC12 -0.4322NM_012128 CLCA4 -0.4324NM_020416 PPP2R2C -0.4325NM_153370 PI16 -0.4326NM_005555 KRT6B -0.4327NM_138803 LOC130940 -0.4327NM_138787 LOC119710 -0.4331NM_012423 RPL13A -0.4332AF075049 AF075049 -0.4334NM_006942 SOX15 -0.4335Contig47308_RC C20orf17 -0.4336NM_015690 STK36 -0.4336NM_001450 FHL2 -0.4338NM_031475 ESPN -0.4338NM_024652 FLJ23119 -0.4338NM_018081 FLJ10385 -0.4343NM_002079 GOT1 -0.4344NM_002295 LAMR1 -0.4345NM_173485 C20orf17 -0.4346NM_005954 MT3 -0.4348NM_006360 GA17 -0.435NM_023944 CYP4F12 -0.4352NM_182508 FLJ40919 -0.4359NM_014666 ENTH -0.4362NM_004994 MMP9 -0.4364NM_139285 GAR17 -0.4364NM_152866 MS4A1 -0.4365NM_173216 SIAT1 -0.4366

5

NM_178562 MGC50844 -0.4367NM_152486 MGC45873 -0.4369NM_000346 SOX9 -0.437NM_025135 KIAA1695 -0.4372AK054808 LOC126520 -0.4373NM_178537 FLJ25045 -0.4376NM_014642 KIAA0036 -0.4381NM_015550 OSBPL3 -0.4381NM_004923 MTL5 -0.4382Contig36285_RC ZFHX2 -0.4384AK022635 AK022635 -0.4385NM_152499 MGC45441 -0.4388NM_005647 TBL1X -0.4389NM_138436 LOC114926 -0.439NM_003890 FCGBP -0.4392NM_138980 MAPK10 -0.4393NM_017984 ZCWPW1 -0.4395NM_170768 ZFP91 -0.4395NM_012243 SLC35A3 -0.4396NM_014851 KIAA0469 -0.4398NM_020211 RGMA -0.4399NM_000274 OAT -0.44NM_024800 NEK11 -0.44AL050021 SLC7A1 -0.4405AL390084 LOC56964 -0.4405NM_152376 FLJ25429 -0.4406ENST00000284158 ENST0000028415 -0.4407Contig12541_RC KIAA1906 -0.441NM_032132 DKFZP434A131 -0.441NM_153269 C20orf96 -0.4411NM_152665 FLJ40873 -0.4414NM_153840 GPR110 -0.4414NM_015415 DKFZP564B167 -0.4414ENST00000296660 ENST0000029666 -0.4416NM_145047 NOR1 -0.4418Contig44068_RC E2F7 -0.4419NM_025057 C14orf45 -0.442AL080120 ODZ4 -0.4423NM_002986 CCL11 -0.4425AL137389 TPTE -0.4425NM_017558 DKFZp434L0850 -0.4427NM_015668 DKFZP434I092 -0.4431NM_002662 PLD1 -0.4432NM_182629 FLJ35107 -0.4434NM_006531 TG737 -0.4435NM_170662 CBLB -0.4435NM_001855 COL15A1 -0.4437AK056178 C20orf121 -0.4437NM_003483 HMGA2 -0.4439NM_002723 PRB4 -0.4441Contig19635_RC -0.4445

AK023829 AK023829 -0.4445Contig42711_RC -0.4446ENST00000229913 ENST000002299 -0.4449NM_138468 LOC130026 -0.4451RSE_00000871014 RSE_000008710 -0.4456NM_025216 WNT10A -0.4457Contig52405_RC EDG3 -0.4458NM_006946 SPTBN2 -0.4458NM_004598 SPOCK -0.4459U28831 KIAA1641 -0.4461NM_001482 GATM -0.4462Contig49606_RC FLJ40298 -0.4465NM_004137 KCNMB1 -0.4468NM_130852 PLUNC -0.4469NM_001998 FBLN2 -0.4474NM_003777 DNAH11 -0.4476NM_145299 LOC200383 -0.4479NM_021179 LOC57821 -0.448ENST00000267844 similar to SHC -0.448NM_032301 MGC10870 -0.4483Contig39328 LOC118491 -0.4484NM_013227 AGC1 -0.4486NM_022448 RGNEF -0.4489ENST00000284765 ENST0000028476 -0.4491NM_024680 FLJ23311 -0.4494NM_004098 EMX2 -0.4498NM_005167 MGC19531 -0.4499NM_152685 SLC23A1 -0.4499NM_020153 FLJ21827 -0.4501NM_080680 COL11A2 -0.4502NM_182495 FLJ25224 -0.4502Contig25938_RC EBF2 -0.4505NM_025184 FLJ22843 -0.4505NM_005688 ABCC5 -0.4508NM_152718 FLJ32009 -0.4508NM_153425 TRADD -0.4509Contig28319_RC -0.451NM_138924 GAMT -0.4511AF131825 NALP1 -0.4515NM_001402 EEF1A1 -0.4515NM_024867 FLJ23577 -0.4517L35592 -0.4522NM_001944 DSG3 -0.4523NM_007173 SPUVE -0.4525Contig45891_RC -0.4525Contig33936_RC MGC35043 -0.4529AK025209 COQ6 -0.4529NM_032929 USP45 -0.4532Contig21465_RC -0.4533NM_006029 PNMA1 -0.4533AL137333 LOC92017 -0.4534

NM_144682 FLJ31952 -0.4536Contig42740_RC FLJ20753 -0.4537ENST00000271463 ENST0000027146 -0.4537NM_175059 NM_175059 -0.4537NM_017794 KIAA1797 -0.4537NM_014443 IL17B -0.4537AF086014 AF086014 -0.4538Contig55883_RC -0.4541AK026805 AK026805 -0.4541NM_130385 MRVI1 -0.4541AL137259 HYDIN -0.4542NM_130897 DNCL2B -0.4542NM_173554 MGC44593 -0.4542NM_015512 DKFZP434A236 -0.4542NM_006235 POU2AF1 -0.4543ENST00000297078 ENST000002970 -0.4544NM_025052 FLJ23074 -0.4544NM_006842 SF3B2 -0.4545NM_153454 C21orf86 -0.4545NM_001941 DSC3 -0.4546NM_152498 FLJ32000 -0.4546NM_001651 AQP5 -0.4549NM_002753 MAPK10 -0.4552NM_181874 GRM7 -0.4555NM_052863 SCGB3A1 -0.4556ENST00000288254 ENST0000028825 -0.4557NM_144698 FLJ25124 -0.4559NM_145046 CALR3 -0.4561NM_003933 BAIAP3 -0.4561NM_015910 LOC51057 -0.4562Contig33416_RC FLJ20972 -0.4564NM_014286 FREQ -0.4565AF086216 SERPINB5 -0.4566NM_018422 DKFZp761K1423 -0.4567Contig36350_RC -0.4568NM_173581 FLJ90231 -0.4568ENST00000296128 ENST0000029612 -0.457NM_145027 C6orf102 -0.4571Contig26760 -0.4573NM_145172 NYD-SP29 -0.4574NM_000597 IGFBP2 -0.4575NM_013300 HSU79274 -0.4576AB037857 PTGFRN -0.4576NM_016950 SPOCK3 -0.4579NM_018679 TCP11 -0.4583NM_001770 CD19 -0.4583NM_144980 C6orf118 -0.4584Contig22599_RC -0.4585Contig38580_RC FARP1 -0.4586NM_138818 LOC158471 -0.4587NM_007050 PTPRT -0.4591

NM_018208 FLJ10761 -0.4591NM_024694 FLJ23121 -0.4592NM_018897 DNAH7 -0.4594NM_012443 SPAG6 -0.4595NM_001302 CORT -0.4595AL359605 -0.4598NM_003480 MAGP2 -0.4599NM_001453 FOXC1 -0.4599NM_024916 CD22 -0.4599NM_145038 MGC16372 -0.4599NM_024838 FLJ22002 -0.4602NM_000640 IL13RA2 -0.4603NM_144577 FLJ32926 -0.4604NM_170665 ATP2A2 -0.4605AK021986 AK021986 -0.4612NM_022128 RBSK -0.4612ENST00000299815 ENST000002998 -0.4612NM_145045 MGC20983 -0.4612NM_017681 FLJ20130 -0.4614NM_003462 DNALI1 -0.4614NM_182826 SCARA3 -0.4619NM_177980 CDH26 -0.4624NM_145314 LOC221044 -0.4627NM_172242 SPAG6 -0.4627Contig40889_RC -0.4628NM_152785 GCET2 -0.4629NM_001500 GMDS -0.4632NM_006860 RABL4 -0.4634NM_001212 C1QBP -0.4636NM_013334 GMPPB -0.4639AB058744 KIAA1841 -0.4639NM_001647 APOD -0.4639NM_014449 GRCA -0.464NM_024593 FLJ11767 -0.464NM_003707 RUVBL1 -0.4642NM_014944 CLSTN1 -0.4643NM_173543 FLJ32844 -0.4647NM_144668 MGC33630 -0.4647Contig31985_RC -0.465NM_005375 MYB -0.465NM_024763 FLJ23129 -0.4654Contig25110_RC -0.4655NM_031457 MS4A8B -0.4655NM_000614 CNTF -0.4656NM_014353 RAB26 -0.4658NM_032483 HTPAP -0.4658Contig36363_RC -0.466NM_152666 FLJ40773 -0.4661NM_174892 TREM5 -0.4662NM_145020 FLJ32743 -0.4662NM_020643 C11orf16 -0.4663

NM_000846 GSTA2 -0.4663NM_152770 MGC35043 -0.4664NM_015143 METAP1 -0.4664NM_001877 CR2 -0.4665ENST00000297220 ENST0000029722 -0.4665NM_152384 DKFZp762I194 -0.4667NM_003746 DNCL1 -0.4668NM_000441 SLC26A4 -0.4669NM_032861 SERAC1 -0.4669Contig63748_RC -0.4674NM_138573 LOC145957 -0.4675NM_019858 GRCA -0.4676NM_014359 OPTC -0.4677AB040929 CNTN3 -0.4679NM_002523 NPTX2 -0.468AK021858 FOXC1 -0.4681NM_032898 MGC14126 -0.4681NM_018837 SULF2 -0.4683AK056999 AK056999 -0.4687NM_032313 MGC3232 -0.4693NM_012188 FOXI1 -0.4693NM_017609 DKFZp434A1721 -0.4695ENST00000296996 ENST0000029699 -0.4695NM_000396 CTSK -0.4696NM_145054 LOC146845 -0.4698NM_016552 ANKMY1 -0.47NM_173672 PPIL6 -0.4701NM_007244 PROL4 -0.4702NM_006087 TUBB5 -0.4705NM_004496 FOXA1 -0.4707NM_018981 ERdj5 -0.4708Contig57441_RC NPR2L -0.4709NM_031472 MGC11134 -0.471NM_018278 KIF24 -0.471NM_177988 MRPL47 -0.4711Contig22551_RC -0.4714NM_003705 SLC25A12 -0.4714NM_004344 CETN2 -0.4715NM_006596 POLQ -0.4717AK055362 AK055362 -0.4717NM_002457 MUC2 -0.4722AL049354 -0.4722NM_017440 MDM1 -0.4722NM_173855 LOC283385 -0.4722ENST00000282823 ENST0000028282 -0.4723NM_020039 ACCN2 -0.4725Contig23169_RC MGC35261 -0.4727NM_018152 C20orf12 -0.4729NM_012210 TRIM32 -0.473NM_152505 MGC33295 -0.4732NM_138705 LOC163688 -0.4735

NM_173476 FLJ34512 -0.4736NM_016606 LOC51308 -0.4737NM_173568 FLJ36335 -0.4739AL122071 C10orf36 -0.4741NM_015310 EFA6R -0.4741Contig16329_RC -0.4742NM_033426 KIAA1737 -0.4742NM_018431 DOK5 -0.4742NM_004242 HMGN3 -0.4743Contig37946_RC EDG7 -0.4744NM_024687 FLJ23049 -0.4744Contig30480_RC -0.4745NM_002250 KCNN4 -0.4746NM_032606 CAPS2 -0.4746NM_003806 HRK -0.4747NM_032832 LRP11 -0.4749NM_002178 IGFBP6 -0.4749NM_053276 VIT -0.475ENST00000290628 ENST0000029062 -0.4752NM_005998 CCT3 -0.4754ENST00000295858 ENST0000029585 -0.4755NM_144992 MGC26733 -0.476AL161972 ICAM2 -0.4762NM_032627 SSBP4 -0.4762NM_152784 MGC39581 -0.4764NM_152645 FLJ22944 -0.4767NM_006088 TUBB2 -0.4768NM_033413 MGC16309 -0.4768NM_006159 NELL2 -0.477Contig34450_RC LOC161577 -0.4772NM_144715 FLJ25200 -0.4773NM_020779 KIAA1336 -0.4773NM_145010 MGC26778 -0.4777NM_053285 TEKT1 -0.4779U80736 TNRC9 -0.4781NM_017835 C21orf59 -0.4782NM_016150 ASB2 -0.4783NM_138770 LOC90557 -0.4784ENST00000287402 ENST0000028740 -0.4785AK024968 BCAS1 -0.4787Contig25944_RC -0.4788AB040926 KIAA1493 -0.4788AL133599 BOC -0.4788NM_024703 FLJ10640 -0.4788NM_173549 FLJ39553 -0.479NM_139163 ALS2CR12 -0.479Contig38906_RC TSARG3 -0.4794Contig47247_RC -0.4794NM_178813 AKAP28 -0.4794Contig23080_RC DKFZP667C165 -0.4795NM_000309 PPOX -0.4795

ENST00000280921 ENST0000028092 -0.4795NM_152632 MGC34831 -0.4796NM_145035 ADMP -0.4799NM_003104 SORD -0.4804NM_018076 FLJ10817 -0.4808NM_032943 SYTL2 -0.4809NM_014395 DAPP1 -0.481NM_004520 KIF2 -0.4811NM_018418 SPATA7 -0.4813NM_014026 DCPS -0.4815NM_012337 NESG1 -0.4816NM_144702 FLJ32884 -0.4816NM_178827 FLJ35834 -0.4817NM_002439 MSH3 -0.4817NM_020817 KIAA1407 -0.4817ENST00000280575 DNAH10 -0.4818NM_175896 LOC283476 -0.4818AB011114 KIAA0542 -0.482NM_014711 CP110 -0.4821NM_032872 JFC1 -0.4824NM_145740 GSTA1 -0.4824NM_020775 KIAA1324 -0.4825NM_032596 C9orf24 -0.4828Contig36499_RC -0.483NM_001036 RYR3 -0.483AB011128 KIAA0556 -0.483NM_173081 FLJ32827 -0.4831Contig31966_RC -0.4832U79242 GRM5 -0.4832ENST00000244841 ENST0000024484 -0.4832NM_177964 NM_177964 -0.4832NM_014466 TEKT2 -0.4838NM_006809 TOMM34 -0.484NM_004662 DNAH9 -0.4842Contig29505_RC FLJ12303 -0.4843NM_013410 AK3 -0.4844NM_139170 LOC146562 -0.4844NM_172367 LOST1 -0.4845NM_023036 DNAI2 -0.4845NM_152327 AK7 -0.4846NM_182902 KIF9 -0.4848Contig45908_RC DKFZp547I048 -0.4851NM_018645 HES6 -0.4851ENST00000292207 ENST0000029220 -0.4852NM_032010 MAP1B -0.4854Contig39492_RC -0.4855NM_000669 ADH1C -0.4857NM_153274 VMD2L2 -0.4858NM_018306 FLJ11036 -0.4861Contig39297_RC LOC148898 -0.4863NM_025019 TUBA4 -0.4867

3

5

NM_152689 MGC9712 -0.4867NM_144978 FLJ32745 -0.4868NM_144644 TSARG2 -0.4868NM_016235 GPRC5B -0.4871NM_152999 STEAP2 -0.4876ENST00000297068 LOC340277 -0.4878NM_002045 GAP43 -0.4879NM_015478 L3MBTL -0.4882NM_173528 FLJ38615 -0.4882Contig1111_RC -0.4883NM_002888 RARRES1 -0.4886NM_173516 PNLDC1 -0.4886NM_182628 FLJ20123 -0.4886NM_015681 EPPB9 -0.489NM_032785 FLJ11588 -0.4891NM_152290 MGC35194 -0.4893NM_012436 SPAG8 -0.4897NM_004878 PTGES -0.4897NM_175922 MGC35308 -0.4897NM_178456 C20orf85 -0.4898NM_002206 ITGA7 -0.49ENST00000222983 ENST0000022298 -0.49NM_004734 DCAMKL1 -0.4901NM_080590 CAPS -0.4902Contig27082_RC -0.4903NM_138722 BCLG -0.4903NM_153270 CNK2 -0.4906NM_001454 FOXJ1 -0.4909NM_018659 C17 -0.491Contig30411_RC OMG -0.491Contig14658_RC -0.491NM_032561 C22orf23 -0.491X17117 MGC27165 -0.491NM_139072 DNER -0.491NM_005460 SNCAIP -0.4911NM_032946 NXF5 -0.4911NM_021777 ADAM28 -0.4912NM_152628 MGC39715 -0.4912NM_153699 GSTA5 -0.4916NM_001882 CRHBP -0.4919NM_152892 DKFZp434K1815 -0.492AL137581 FLJ34497 -0.4921NM_152548 FLJ25333 -0.4921NM_152377 MGC34837 -0.4922NM_133170 PTPRT -0.4922NM_018409 DKFZp761O011 -0.4924NM_001808 CELP -0.4925NM_005106 DLEC1 -0.4926NM_031421 DKFZP434H011 -0.4928NM_144647 MGC26610 -0.4928NM_138796 LOC128153 -0.4929

5

NM_018718 TSGA14 -0.493ENST00000288276 ENST000002882 -0.493AL049365 FTL -0.4931NM_024926 FLJ12571 -0.4931AK058070 LOC130752 -0.4933NM_018423 DKFZp761P1010 -0.4934NM_022742 DKFZP434G156 -0.4934Contig4382_RC MAP1B -0.4936NM_032809 C9orf54 -0.4936NM_032133 DKFZp434N141 -0.4936NM_145913 SLC5A8 -0.4936Contig56270_RC FBXO32 -0.4937Contig48473_RC LOC120376 -0.4938NM_153244 MGC35468 -0.4941NM_003613 CILP -0.4942NM_182510 FLJ32252 -0.4947NM_022138 SMOC2 -0.495NM_005980 S100P -0.4951Contig38614_RC -0.4953NM_145755 STI2 -0.4953ENST00000281357 ENST0000028135 -0.4954NM_002628 PFN2 -0.4956NM_001852 COL9A2 -0.4956NM_152761 FLJ25444 -0.4959NM_144965 FLJ32780 -0.4961AB051484 FLJ23529 -0.497NM_022784 FLJ12476 -0.497NM_015896 ZMYND10 -0.4971NM_178033 CYP4X1 -0.4972ENST00000229554 RSHL3 -0.4973NM_182766 FLJ32940 -0.4975NM_144570 C16orf34 -0.4976NM_018399 VNN3 -0.4977NM_005114 HS3ST1 -0.4979NM_000673 ADH7 -0.4982AB002365 KIAA0367 -0.4983NM_033548 MGC12518 -0.4985NM_013412 RABL2A -0.4985NM_032471 PKIB -0.4986NM_016172 GBDR1 -0.4987NM_014157 HSPC065 -0.4987NM_173559 FLJ25791 -0.4987NM_018272 LAS1 -0.4991NM_000096 CP -0.4991NM_032794 C6orf29 -0.4993NM_001107 ACYP1 -0.5ENST00000278924 ENST0000027892 -0.5004NM_032165 NM_032165 -0.5004NM_152534 FLJ32685 -0.5007NM_173565 LOC222967 -0.5009NM_007081 RABL2B -0.501

NM_015417 C20orf28 -0.5011NM_145286 STOML3 -0.5011NM_080860 TSGA2 -0.5012Contig50106_RC KIF21A -0.5013NM_024059 MGC5356 -0.5014NM_017821 RHBDL2 -0.5016Contig54581_RC TSBF1 -0.5018NM_181676 PPP2R2B -0.5019AB018332 KIAA0789 -0.502NM_003035 SIL -0.5024NM_005855 RAMP1 -0.5028NM_002864 PZP -0.5032NM_182832 PLAC4 -0.5032Contig48935_RC -0.5033NM_002589 PCDH7 -0.5034NM_033495 BKLHD2 -0.5034NM_032490 C14orf142 -0.5034NM_145170 LOC118491 -0.5035NM_145263 LOC132671 -0.5037NM_174949 NM_174949 -0.5037Contig48764_RC -0.504NM_025244 TSGA10 -0.504NM_130446 KLHL6 -0.5047NM_032118 FLJ12953 -0.505NM_016311 ATPIF1 -0.5051NM_031916 ASP -0.5052NM_181643 LOC128344 -0.5052Contig43096_RC -0.5053NM_138790 LOC122618 -0.5056NM_018387 STRBP -0.5057ENST00000286784 ENST0000028678 -0.5057NM_138451 LOC115811 -0.506NM_006083 IK -0.506NM_013989 DIO2 -0.5061NM_145309 LOC220074 -0.5062NM_031956 NYD-SP14 -0.5063NM_152732 C6orf206 -0.5064NM_178504 FLJ40427 -0.5064NM_005769 CHST4 -0.5067AK057637 AK057637 -0.5072Contig26436_RC -0.5074NM_006113 VAV3 -0.5077Contig12742_RC FLJ42117 -0.5079AB032983 KIAA1157 -0.508NM_175903 LOC284033 -0.508NM_001847 COL4A6 -0.508NM_181656 LOC284018 -0.5081Contig55814_RC MOPT -0.5083NM_031422 CHST9 -0.5084NM_152366 MGC33338 -0.5085AL137578 -0.5087

6

NM_138409 C6orf117 -0.5088Contig49811_RC FLJ46909 -0.509NM_178821 FLJ25955 -0.5091NM_152325 MGC40178 -0.5094NM_178545 LOC339456 -0.5096NM_000429 MAT1A -0.5097Contig33121_RC EPN3 -0.5099NM_003712 PPAP2C -0.51NM_001717 BNC -0.5102NM_058242 KRT6C -0.5106NM_001085 SERPINA3 -0.5107NM_001069 TUBB -0.511NM_005932 MIPEP -0.5112NM_002407 SCGB2A1 -0.5113NM_133443 GPT2 -0.512NM_003296 CRISP2 -0.5123NM_178837 LOC352909 -0.5123NM_016338 IPO11 -0.5123NM_013259 NP25 -0.5126NM_153353 MGC27085 -0.5127NM_024548 FLJ23047 -0.5127NM_017957 EPN3 -0.513NM_015653 DKFZP566F054 -0.5132Contig2657_RC MGC16207 -0.5133NM_032411 ECRG4 -0.5136NM_019077 UGT1A7 -0.5136NM_030941 LOC81691 -0.5137NM_003382 VIPR2 -0.5137NM_024549 FLJ21127 -0.5139NM_178824 FLJ33620 -0.514NM_145026 SPATS1 -0.514Contig48613_RC PCDH7 -0.5144NM_006443 C6orf108 -0.5145AK021527 -0.5145NM_152291 MUC7 -0.5145NM_152322 BTBD11 -0.5146NM_018430 TSNAXIP1 -0.5147AB040938 KIAA1505 -0.5149NM_152750 FLJ23834 -0.5149NM_000463 UGT1A1 -0.5155NM_152773 MGC33212 -0.5155NM_025059 C6orf97 -0.5157NM_020873 LRRN1 -0.516Contig41585_RC C6orf198 -0.5161NM_181696 PRDX1 -0.5163NM_018602 DNAJA4 -0.5168RSE_00000160398 RSE_0000016039 -0.5173NM_152676 FBXO15 -0.5175NM_000436 OXCT -0.5177NM_016307 PRRX2 -0.5178NM_003551 NME5 -0.5179

AB051471 KIAA1684 -0.518AK024495 DKFZp761L1518 -0.5181NM_006217 SERPINI2 -0.5182NM_003059 SLC22A4 -0.5183NM_001231 CASQ1 -0.5183NM_174978 C14orf39 -0.5185AK023156 -0.5186NM_020707 KIAA1173 -0.5187NM_058180 C21orf58 -0.5188NM_018194 FLJ10724 -0.5188NM_181645 FLJ25393 -0.519Contig41398_RC FLJ46675 -0.5193NM_016932 SIX2 -0.5195NM_024783 FLJ23598 -0.52NM_001345 DGKA -0.52NM_018100 EFHC1 -0.5204NM_000300 PLA2G2A -0.5204NM_080475 SERPINB11 -0.5205NM_032930 MGC13040 -0.5206NM_153376 FLJ90575 -0.5207NM_012450 SLC13A4 -0.5208NM_005982 SIX1 -0.5212AL137334 LOC123872 -0.5212NM_016126 LOC51668 -0.5214NM_000817 GAD1 -0.5217NM_001719 BMP7 -0.5218NM_153020 RNPC6 -0.5218NM_002905 RDH5 -0.5222Contig46563_RC -0.5226NM_031461 LOC83690 -0.5227NM_006648 PRKWNK2 -0.5227NM_017425 SPA17 -0.5228NM_152410 PACRG -0.5232NM_032263 DKFZp434B227 -0.5243AB007880 ALG1 -0.5244NM_130840 ATP6V0A4 -0.5246NM_144654 MGC29761 -0.5248NM_032457 PCDH7 -0.5248NM_152500 FLJ33084 -0.5251NM_002113 HFL1 -0.5253X56210 HFL2 -0.5254Contig27175_RC -0.5254NM_014265 ADAM28 -0.5254NM_001189 BAPX1 -0.5255AK001139 AK001139 -0.5258NM_018658 KCNJ16 -0.5265Contig50891_RC PAK1 -0.5265NM_002373 MAP1A -0.5265Contig31424_RC C6orf154 -0.5268NM_021225 PROL1 -0.5269NM_170742 KCNJ16 -0.5269

0

NM_016364 DUSP13 -0.527NM_173662 LOC285533 -0.5271NM_177532 RASSF6 -0.5272NM_005830 MRPS31 -0.5274NM_005080 XBP1 -0.5278NM_004473 FOXE1 -0.528Contig38529_RC KIAA1889 -0.5281NM_004914 RAB36 -0.5281NM_182634 FLJ36166 -0.5281NM_000793 DIO2 -0.5286NM_145032 MGC21636 -0.5289NM_153208 MGC35048 -0.529NM_003106 SOX2 -0.529NM_033124 NYD-SP28 -0.5292NM_001298 CNGA3 -0.5292Contig53033_RC LOC119587 -0.5293NM_000454 SOD1 -0.5293NM_006608 PHTF1 -0.5297NM_173582 FLJ32029 -0.5297NM_178550 MGC48998 -0.5298NM_031294 DKFZP586M112 -0.5299AK056976 STARS -0.5299NM_173605 KCNRG -0.5304NM_004058 CAPS -0.5305NM_000186 HF1 -0.5305NM_004046 ATP5A1 -0.5307NM_138789 LOC120379 -0.5309NM_144707 PROM2 -0.5309NM_178012 MGC8685 -0.531NM_138812 LOC143241 -0.5317Contig40831_RC -0.5322NM_021778 ADAM28 -0.5332NM_031456 C17orf1A -0.5333NM_032269 DKFZp434I099 -0.5334NM_006250 PRH1 -0.5335NM_181613 B7 -0.5336NM_178190 ATPIF1 -0.5341NM_172366 FBXO16 -0.5341AK027233 AK027233 -0.5342NM_001140 ALOX15 -0.5345NM_000348 SRD5A2 -0.5352NM_024053 C22orf18 -0.5353Contig38911_RC -0.5354NM_015585 C20orf26 -0.5357NM_033150 COL2A1 -0.5358NM_144648 FLJ32786 -0.5361NM_003657 BCAS1 -0.5362NM_004751 GCNT3 -0.5364NM_058179 PSAT1 -0.5371NM_032304 HAGHL -0.5378NM_144605 FLJ25410 -0.5379

ENST00000283902 ENST0000028390 -0.5382X98307 SERPINB13 -0.5388NM_024603 FLJ11588 -0.539NM_005672 PSCA -0.5395NM_000782 CYP24A1 -0.5396NM_021151 CROT -0.5396NM_006952 UPK1B -0.5405NM_019028 HIP14L -0.5406NM_001063 TF -0.5409NM_006536 CLCA2 -0.541NM_080661 MGC15937 -0.5414NM_152673 MUC20 -0.5418Contig49591_RC ATF7IP2 -0.5419NM_021800 JDP1 -0.5427NM_003695 E48 -0.5428NM_000392 ABCC2 -0.5429NM_006061 CRISP3 -0.5429NM_145814 CACNG6 -0.5429NM_052831 C6orf192 -0.5431NM_033342 TRIM7 -0.5431Contig27818_RC HNF4G -0.5433NM_015533 DKFZP586B1621 -0.5436NM_023007 FLJ12517 -0.5437NM_000662 NAT1 -0.5439NM_152680 FLJ32028 -0.544AB040959 CIP98 -0.5441NM_032131 ARMC2 -0.5441NM_024697 FLJ22419 -0.5443NM_019075 UGT1A10 -0.5447NM_001498 GCLC -0.5449NM_005727 TSPAN-1 -0.545Contig2313_RC DKFZp667B0210 -0.5456Contig54365_RC EHF -0.5456NM_138634 MSMB -0.5458NM_019093 UGT1A3 -0.5462NM_015873 VILL -0.5465NM_021147 UNG2 -0.5466NM_145235 FANK1 -0.5478Contig52114_RC HTPAP -0.548NM_018147 FAIM -0.5481NM_002615 SERPINF1 -0.5486AK002107 RAB3B -0.5493NM_001710 BF -0.5495NM_002202 ISL1 -0.5495NM_002443 MSMB -0.5497NM_005629 SLC6A8 -0.5499NM_000480 AMPD3 -0.5508NM_017539 DNAH3 -0.5509NM_018414 SIAT7A -0.551ENST00000256921 KCTD1 -0.5512AK002208 -0.5517

NM_032257 ZMYND12 -0.552NM_153212 GJB4 -0.5522NM_004051 BDH -0.5529NM_004237 TRIP13 -0.5532NM_005013 NUCB2 -0.5533NM_003355 UCP2 -0.5533NM_182848 CLDN10 -0.5534NM_024889 FLJ23537 -0.5535NM_015931 LOC51066 -0.5539NM_001072 UGT1A6 -0.5543NM_000681 ADRA2A -0.5546NM_003645 SLC27A2 -0.5551NM_002993 CXCL6 -0.5556NM_021027 UGT1A9 -0.5556NM_003256 TIMP4 -0.5559NM_007120 UGT1A4 -0.5561NM_024997 ATF7IP2 -0.5566NM_006384 CIB1 -0.5567NM_052924 RHPN1 -0.5567NM_181875 GRM7 -0.5572NM_000160 GCGR -0.5577NM_001197 BIK -0.5581AK024864 AK024864 -0.5586NM_019076 UGT1A8 -0.5588NM_006790 TTID -0.5593NM_001262 CDKN2C -0.5596NM_019894 TMPRSS4 -0.5599NM_001793 CDH3 -0.5604NM_003066 SLPI -0.5611Contig29193_RC -0.5611NM_000503 EYA1 -0.5612NM_006194 PAX9 -0.5615NM_000775 CYP2J2 -0.5617NM_152701 ABCA13 -0.5626NM_003064 SLPI -0.563NM_152997 C4orf7 -0.564NM_017738 C9orf39 -0.5652Contig28286_RC -0.5652NM_173815 NM_173815 -0.5655NM_030567 MGC10772 -0.5656NM_004883 NRG2 -0.5657NM_172365 C14orf50 -0.5657NM_013230 CD24 -0.5667Contig44656_RC -0.5678NM_178175 LHFPL1 -0.5694NM_001236 CBR3 -0.5701NM_021615 CHST6 -0.5704ENST00000278207 ENST0000027820 -0.5705NM_006984 CLDN10 -0.5708NM_130898 CREB3L4 -0.5712NM_004616 TM4SF3 -0.5717

NM_016425 TMPRSS4 -0.5719AK055485 AK055485 -0.5719NM_014638 KIAA0450 -0.5722NM_020990 CKMT1 -0.5725NM_152450 MGC26690 -0.573AF086374 AF086374 -0.5744NM_014433 RTDR1 -0.5749NM_172240 TUWD12 -0.5755NM_000420 KEL -0.5758NM_001831 CLU -0.5764Contig39045_RC -0.577NM_012391 SPDEF -0.5783Z48314 MUC5AC -0.5787NM_005368 MB -0.5789NM_006512 SAA4 -0.58ENST00000276154 ENST0000027615 -0.5801NM_001751 CARS -0.5804Contig33767_RC NS5ATP13TP2 -0.5805NM_006017 PROM1 -0.5805NM_024626 B7-H4 -0.5805Contig20629_RC -0.5806Contig57081_RC MSI2 -0.5808Contig66573_RC FLJ12567 -0.5809NM_013981 NRG2 -0.5815NM_000233 LHCGR -0.5821NM_006614 CHL1 -0.5827NM_016548 GOLPH2 -0.5829AB029009 KIAA1086 -0.583NM_144613 COXVIB2 -0.583NM_181814 C14orf29 -0.5835AK022390 AK022390 -0.5838NM_014278 APG-1 -0.5839NM_032380 EFG2 -0.584NM_020646 ASCL3 -0.5846NM_152599 FLJ35773 -0.5857Contig37501_RC -0.5866NM_014850 SRGAP2 -0.5866U39573 LPO -0.5868Contig41669_RC -0.5868NM_175887 LOC222171 -0.5869NM_017878 HRASLS2 -0.5871NM_013982 NRG2 -0.5872Contig44729_RC -0.5872NM_003154 STATH -0.5887NM_152623 FLJ37927 -0.5888NM_003038 SLC1A4 -0.5889NM_001853 COL9A3 -0.5889Contig29482_RC FOXN4 -0.589AK024927 AK024927 -0.5896NM_004572 PKP2 -0.59NM_018692 C20orf17 -0.5901

NM_004527 MEOX1 -0.5908NM_145051 MGC4734 -0.5914NM_005476 GNE -0.5923NM_172110 EYA2 -0.5924NM_015678 NBEA -0.5925NM_003722 TP73L -0.5925NM_002627 PFKP -0.5928NM_013999 MEOX1 -0.5932NM_173807 MGC33370 -0.5939NM_000693 ALDH1A3 -0.5942Contig49319_RC -0.5943Contig47879_RC -0.5949NM_001095 ACCN2 -0.5953NM_000474 TWIST1 -0.5956NM_004858 SLC4A8 -0.5982NM_004887 CXCL14 -0.5988NM_003226 TFF3 -0.5988NM_006919 SERPINB3 -0.6008ENST00000297667 ENST0000029766 -0.6009NM_002275 KRT15 -0.6014NM_002851 PTPRZ1 -0.6018NM_025071 MSF -0.6019NM_000774 CYP2F1 -0.602Contig33811_RC -0.6025ENST00000293937 ENST0000029393 -0.6032NM_006516 SLC2A1 -0.6037NM_023932 EGFL9 -0.6039NM_007281 SCRG1 -0.6044NM_148672 CCL28 -0.605Contig48834_RC TINP1 -0.6054NM_030754 SAA2 -0.6056NM_012105 BACE2 -0.6062NM_025034 USH1C -0.6062NM_138569 C6orf142 -0.607NM_017578 ROPN1 -0.6073NM_001676 ATP12A -0.6081NM_001062 TCN1 -0.6083NM_002425 MMP10 -0.6087AK001432 -0.6092NM_023915 GPR87 -0.6098Contig42355_RC SLC25A21 -0.6099NM_181533 C14orf29 -0.6115Contig36606_RC -0.6123AF308287 CLN6 -0.6123NM_033197 C20orf114 -0.6138AK056995 AK056995 -0.6155NM_003225 TFF1 -0.616AL137310 DKFZp761E1312 -0.6173NM_005268 GJB5 -0.6178NM_019846 CCL28 -0.6179Contig51943_RC -0.62

NM_147204 TRPV4 -0.6201NM_024122 MGC4825 -0.6209NM_005709 USH1C -0.6212NM_020659 TTYH1 -0.6221NM_001267 CHAD -0.6235NM_016358 IRX4 -0.625Contig25319_RC -0.6251NM_145016 BXMAS2-10 -0.6257NM_005771 DHRS9 -0.6264NM_022369 FLJ12541 -0.6265NM_000150 FUT6 -0.6284NM_152672 OSTalpha -0.6287NM_021240 DMRT3 -0.6287NM_014211 GABRP -0.6288AB007969 KIAA0500 -0.6306NM_138397 LOC93082 -0.6314NM_007063 TBC1D8 -0.6314NM_002652 PIP -0.6319NM_025165 ELL3 -0.6321NM_005042 PRH2 -0.6323NM_139273 CARS -0.6326Contig49342_RC GALNT6 -0.6335NM_000422 KRT17 -0.6339NM_003778 B4GALT4 -0.6352NM_182763 MCL1 -0.6368NM_012447 STAG3 -0.6375NM_032865 CTEN -0.6405NM_005021 ENPP3 -0.6416NM_006408 AGR2 -0.6427NM_152428 MGC35285 -0.6434NM_015515 KRT23 -0.6437NM_012190 FTHFD -0.6458NM_024690 MUC16 -0.6466NM_138991 BACE2 -0.6489NM_138299 MUC4 -0.6493NM_000094 COL7A1 -0.6508NM_014256 B3GNT3 -0.6531NM_000661 RPL9 -0.654Contig47381_RC -0.6547AF075063 AF075063 -0.6551NM_005564 LCN2 -0.6554NM_002343 LTF -0.6589AK026761 AK026761 -0.661NM_000795 DRD2 -0.6636NM_024734 CLMN -0.6657NM_005978 S100A2 -0.6662NM_000424 KRT5 -0.6712NM_012101 TRIM29 -0.672NM_007210 GALNT6 -0.6721NM_002639 SERPINB5 -0.6725NM_004252 SLC9A3R1 -0.6758

NM_000331 SAA1 -0.6787NM_138805 FAM3D -0.6831NM_001723 BPAG1 -0.6873NM_022112 P53AIP1 -0.6937NM_024423 DSC3 -0.6939NM_002974 SERPINB4 -0.6942NM_145252 LOC124220 -0.6972NM_032034 SLC4A11 -0.6988NM_017648 MUC13 -0.7045NM_130778 COL17A1 -0.7075NM_025153 FLJ21477 -0.7088NM_033049 MUC13 -0.7091AK021694 CXorf10 -0.7098NM_006533 MIA -0.7156NM_006103 WFDC2 -0.7276NM_001961 EEF2 -0.7923NM_000968 RPL4 -0.7971