gary killian, curriculum vitae

Gary Killian, Curriculum Vitae

EDUCATION DEGREE YEAR FIELD OF STUDY

Kansas State University B.S. 1967 Zoology

Kansas State University M.S. 1969 Embryology

Penn State University Ph.D. 1973 Reproductive Physiology

Los Alamos National Lab Sabbatical 2001 Flow Cytometry

EMPLOYMENT EXPERIENCE

Kansas State University

Teaching Assistant 1967-1969

Penn State University

Graduate Assistant 1969-1971

NSF Trainee 1971-1972

Research Assistant 1972-1973

Assistant Professor of Biology 1973-1974

Research Associate in Dairy Science 1974-1975

Assistant Professor of Dairy Science 1975-76

Kent State University

Assistant Professor of Biology 1976-1980

Associate Professor of Biology 1980-1984

Penn State University

Associate Professor of Animal Science 1984-89

Professor of Reproductive Physiology 1989-2000

University Distinguished Professor of Reproductive Physiology 2001-2006

Emeritus Professor of Reproductive Physiology 2007-present

United States Department of Agriculture Wildlife Services

National Wildlife Research Center

Project Coordinator, 2007-present

PROFESSIONAL ORGANIZATIONS

Phi Kappa Phi

Society for the Study of Reproduction

American Society of Andrology

Wildlife Society

TEACHING EXPERIENCE

In the early years of his career at Penn State (1973-76), Dr. Killian taught Mammalian Anatomy,

Mammalian Physiology, Zoology, and Biological Sciences I, II, and IV, and Reproductive

Physiology Laboratory. He also gave several lectures each year on male reproductive physiology in

the graduate course Physiology 573. From 1976-1984, while a faculty member in the Department of

Biological Sciences at Kent State University, he taught an undergraduate course in Human

Physiology and a graduate course in Reproductive Physiology. Both courses had laboratory sections

in which Dr. Killian was actively involved in teaching. During the eight years he taught Human

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Physiology approximately 2000 students took the course. In 1982, he received the College of Arts

and Sciences Distinguished Teaching Award.

After returning to Penn State in 1984 Dr. Killian’s “official” faculty appointment has had more

emphasis on research, with resident education accounting for only 15-25% effort. Nevertheless,

each year he has taught 1-2 sections of Reproductive Techniques in Cattle. This popular, high

demand elective course emphasizes “hands on” learning experiences for students to gain practical

experience with artificial insemination techniques and pregnancy palpation. For several years he

also was involved in teaching Animal Science Colloquium for undergraduates. On alternate years he

offers a graduate course in Gamete Physiology, which focuses on the cell biology of the gametes

and fertilization. In many of the years since 1984 he has given lectures on male reproductive

physiology in the graduate course Physiology 573.

In 1986, Dr. Killian and his graduate students were responsible for developing the first Animal

Physiology course for students in the Governor’s School for Agriculture. This course was taught

each summer for 5 years to 65 scholars to provide them with “hands on” laboratory experiences for

in vitro fertilization and embryo development, as well as an appreciation for the use of reproductive

technology in cattle. Some 16 years after the course was developed, another faculty member is still

teaching this very popular course.

Throughout his career, Dr. Killian has had a genuine interest in providing research experiences for

undergraduate students either through employment (3-4 students per year) and or individual research

projects for credit (3-4 students per year).

SERVICE AT PENN STATE 1990-2006

Dr. Killian has served on numerous committees at the university, college, and department levels.

The committee assignments reflect his interests in graduate education, research and inter college

programs such as the Life Sciences Consortium and Physiology. At the college level his committee

assignments have focused on service in the area of diversity. In the department he has served on

committees in a variety of areas in graduate and undergraduate studies as well as faculty search

committees, faculty advisory, and promotion and tenure committees.

University: Research Initiation Grant Proposal Review Committee, Graduate Council, Graduate

Council Fellowship Committee, Graduate Council Research Committee, Physiology Program

Steering Committee, Physiology Program Candidacy Exam Committee-Chairman, Graduate

Council Subcommittee for Post-doc Appointments at PSU-Chairman, Graduate Research Exhibition

Committee, AD-14 Review of Director LAR, Physiology Program Course Review Committee,

Large Animal Attending Veterinarian Advisory Committee, Life Sciences Consortium Faculty

Steering Committee, Faculty Scholar Selection Committee, Conflict of Interest Committee

College of Agricultural Sciences: Diversity Advisory Committee, Ad Hoc Selection Committee-

Diversity Achievement Award, Diversity Planning Committee, Diversity Achievement Award

Selection Committee, Graduate Student Travel Awards Committee, Graduate Council

Subcommittee on New and Revised Programs and Courses, Distinguished Lecturer Selection

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Committee-Chairman, College Diversity Task Force, Reproductive Biology Initiative Search

Committee (Chair)

Department of Dairy and Animal Science: Animal Science Candidacy Exam Committee, Student

Recruitment Brochure Committee, Telephone Committee, Reproduction Course Revisions

Committee-Chairman, Distinguished Alumni Selection Committee, Dairy Breeding Research Center

Operations Report Committee-Chairman, Faculty Advisory Committee, Promotion and Tenure

Committee, Horse Operations Report Committee, Golden Anniversary of AI Committee-Chairman,

Undergraduate Program Committee, Graduate Student Research Award Committee-Chairman,

Dairy Facilities Committee, Awards Committee-Chairman, Support Unit Users Group, Deer

Research Center-Chairman, Undergraduate Studies Committee, Graduate Program Committee,

DBRC 50th

Anniversary Celebration-Chairman, Honors Thesis Advisor for Department, Promotion

and Tenure Committee-Poultry Science, LSC Faculty Position Search Committee-Chairman,

Transgenic Advisory Committee, Genomics Position Search Committee, Promotion and Tenure

Committee (DAS)

Efforts to Enhance Equal Opportunity and Cultural Diversity: Dr. Killian has been involved since

1979 with research training of minority students at the undergraduate and graduate levels. In 1981

and 1982, he hosted one student each summer for 10 weeks for the NIH Minority High School

Student Research Apprenticeship Program at Kent State. During the summers of 1985, 1986 and

1987, the candidate was a faculty instructor in the Agricultural Institute for Minority Students

(AIMS) program in the College of Agriculture. In 1986 Dr. Killian's laboratory hosted an AIMS

student for two weeks of research training in the summer. In the summers of 1988, 1989, 1990 the

candidate's laboratory hosted a minority student for 6 weeks, as part of the Minority Student

Apprenticeship in Agriculture Program at PSU. At the graduate level, he trained one M.S. and one

Ph.D. minority student while at Kent State and 3 M.S. and 2 Ph.D. minority students at PSU. In

November of 1992, Dr. Killian's contributions to enhancing equal opportunity and cultural diversity

were recognized by the College of Agricultural Sciences when they presented him with the College's

first Annual Diversity Achievement Award. He also served as mentor and research supervisor for

an undergraduate minority student who participated in the CURO program. (1997-1998) and as

Mentor and Research Supervisor for an SROP Scholar Minority Student during 2 Months in

Summer (1999). Since 1999 he is one of 11 faculty in the University who serve as a Sloan

Foundation Fellowship Mentor. The Sloan Foundation provides fellowships to minority Ph.D.

students in the sciences. Dr. Killian currently serves as Sloan mentor for 4 minority graduate

students.

AWARDS AND HONORS

NSF Predoctoral Trainee, 1970-72; Summer Research Fellowship, 1977; American Men and

Women of Science; World Health Organization Recognition for Training Program in Reproduction,

1981; College of Arts and Sciences Distinguished Teaching Award, 1982; Research and Creative

Activity Award, 1984-85; Reviewer, USDA Competitive Grants Program, Reproductive Biology

Panel Member, 1992; College of Agricultural Sciences Annual Diversity Achievement Award,

1993, NIH- NICHHD Reproductive Biology Study Section, 1994, 1996, 1998, 2003. Named

University Distinguished Professor of Reproductive Physiology in January 2001. Gamma Sigma

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Delta Research Award, 2002; Scientific Advisory Committee, USGS: Development of contraceptive

agents as a management tool to limit fertility in wild horses, 2001-02.

GRADUATE and POSTDOCTORAL STUDENTS ADVISED

Major advisor to 14 M.S. students, 17 Ph.D. students, 25 post-doctoral students/visiting professors.

RESEARCH BIOGRAPHY OF DR. GARY KILLIAN

Dr. Killian’s general research goal is to understand and define factors that influence the fertility of

mammals at the level of the gametes. Fertilization is a membrane related event that results from the

fusion of the spermatozoan and egg. At the cellular level, the success of fertilization is influenced

by signaling and effector molecules that ensure the sperm-egg binding and fusion will occur. Dr.

Killian has been interested in identifying molecules that affect these events, because their

identification may lead to methods for improving livestock or human fertility, or strategies for

contraceptive development in wildlife or humans.

Dr. Killian’s research career provides repeated evidence of originality and creativity. Early in his

career at Penn State (1974-1976) he was the first scientist in his field to develop techniques to

isolate epithelial cells from the epididymis for in vitro study. The epididymis of the male

reproductive tract is the site where sperm become motile and acquire the ability to fertilize an egg.

The techniques developed by Dr. Killian enabled studies aimed at defining secretions of the

epididymis, and how these secretions might influence sperm function.

After returning to Penn State in 1984, Dr. Killian focused his efforts almost entirely on cattle, taking

advantage of the unique facilities available for studying bulls and cows. He and veterinarian Dr.

Kavanaugh were the first to develop a dual cannulation technique that involves placing catheters in

the isthmus and ampulla of the same oviduct for fluid collection throughout the estrous cycle. Fluid

obtained from these catheters was analyzed biochemically and used to study how the components of

the fluid interact with spermatozoa and eggs. These studies led to discoveries by his students that a

specific protein was secreted by the oviduct ampulla around estrus and ovulation, that this protein

was bound by the sperm membrane and egg zona pellucida, and that this protein was able to rapidly

prepare sperm for fertilization in vitro. Collectively, these studies have shed considerable light on

how the oviduct influences the gametes during events surrounding fertilization.

The efforts of Dr. Killian’s research group also are directed toward understanding what components

of the male reproductive system contribute to fertility. One innovative approach has involved

methods for cannulation of the vasa deferentia of bulls, to provide a chronic supply of accessory sex

gland fluid and cauda epididymal sperm and fluid to assess how each fluid contributes to the fertility

of sperm. These studies were the first to demonstrate that accessory sex gland fluid influences the

fertility of sperm at a level that corresponds with the fertility of the bull. In addition, two proteins

have been identified in semen that are associated with high fertility, and two proteins have been

identified as prevalent in lower fertility animals. Studies are in progress to develop a diagnostic test

for predicting sub fertility in young bulls, for which a patent has been issued.

Dr. Killian’s students and lab associates have successfully identified and purified the two high

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fertility proteins from semen and tentatively identified one of the lower fertility proteins. An

application being explored is the addition of high fertility proteins to semen to improve sperm

fertility. These practical applications are not only relevant to the cattle industry, since the three

fertility proteins identified in bulls have also been detected in the semen of stallions, dogs and

humans.

Dr. Killian has also had a long standing interest in contraceptive development, particularly as it is

applied to overabundant wildlife and feral species. Working with colleagues at the National Wildlife

Research Center he has been involved in the development and testing of contraceptive vaccines for

feral swine, white-tailed deer and wild horses. These studies have led to the development of a

single-shot GnRH vaccine that has proven highly effective in contraception of these species. Studies

underway or recently completed involve contraception of penned wild pigs in Florida and wild

horses in Nevada.

The impact of Dr. Killian’s research and mentoring efforts are evident on several levels. He has

served as advisor to 14 M.S. students, 16 Ph.D. students, 21 post-doctoral students/visiting

professors and 5 Honors thesis students. Dr. Killian has authored/co-authored more than 215 papers

and abstracts in leading scientific journals of his field. He has received numerous invitations to

speak in other countries about his research. In addition, he has been invited collaborate on research

projects in Holland, Denmark, New Zealand, Japan, Sweden, Italy, the United Kingdom, Australia

and Brazil. He has consistently, throughout his career, maintained significant grant funding from

both government agencies and industry. This funding has enabled pursuit of both fundamental and

applied research objectives leading to new discoveries and potential applications. These funding

successes attest to the quality of science conducted and to the potential practical benefits derived of

the research in animal agriculture, and animal and human fertility. Articles Published in Refereed Journals 1972-Present

1. Killian, G. J. and R. P. Amann. Reproductive capacity of dairy bulls. IX. Changes in

reproductive organ weights and semen characteristics of Holstein bulls during the first thirty weeks

after puberty. J. Dairy Sci. 55:1631-1635. (1972).

2. Amann, R. P., G. J. Killian and A. W. Benton. Differences in the electrophoretic

characteristics of bovine rete testis fluid and cauda epididymal plasma. J. Reprod. Fertil. 35:321-

330. (1973).

3. Amann, R. P. and G. J. Killian. Electrophoretic and immunological differences between

bovine rete testis fluid and cauda epididymal plasma. Proc. VII Inter. Congr. Anim. Reprod.,

Munich. 3:2239-2241. (1973).

4. Killian, G. J. and R. P. Amann. Immunoelectrophoretic characterization of fluid and sperm

entering and leaving the bovine epididymis. Biol. Reprod. 9:489-499. (1973).

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5. Killian, G. J. and R. P. Amann. Detection of 3α-, 3β- and 17β-hydroxy-5β-steroid

dehydrogenases in epididymal sperm of Holstein bulls. J. Reprod. Fertil. 38:59-64. (1974).

6. Killian, G. J. and R. P. Amann. Reproductive capacity of dairy bulls. X. Changes in

chemical components and immunoelectrophoretic characteristics of seminal plasma before and after

puberty. J. Dairy Sci. 57:703-706. (1974).

7. Killian, G. J. and R. P. Amann. Histochemical localization of neutral lipid and choline-

containing phospholipid in the epididymides of neopuberal Holstein bulls. Biol. Reprod. 11:198-

204. (1974).

8. Killian, G. J. and R. P. Amann and J. Snyder. Isolation of principal and basal cells from the

epithelium of the hamster caput epididymidis by unit gravity sedimentation. Biol. Reprod. 15:266-

279. (1976).

9. Killian, G. J., J. Snyder, and R. P. Amann. Application of unit gravity sedimentation for

separation of cells comprising the rat caput epididymidis. Cell and Tissue Research. 183:371-378.

(1977).

10. Kemp, W. R. and G. J. Killian. Glycosidase activity in epididymal epithelial cells isolated

from normal and α-chlorohydrin treated male rats. Contraception. 17:93-101. (1978).

11. Robb, G. W., R. P. Amann and G. J. Killian. Daily spermatozoal production and epididymal

sperm reserves of neopuberal and adult Wistar rats. J. Reprod. Fertil. 54:103-107. (1978).

12. Killian, G. J. and D. A. Chapman. Glycerlyphosphorylcholine, sialic acid and protein levels

in epithelial cells isolated from the rat caput epididymidis by elutriation. Biol. Reprod. 22:846-850.

(1980).

13. Gelerinter, E., S. Narra and G. J. Killian. Effect of ejaculation frequency on the water

volume of rabbit spermatozoa as determined by electron spin resonance spectroscopy. Biol. Reprod.

23:513-517. (1980).

14. Hoffman, D. S. and G. J. Killian. Isolation of epithelial cells from the corpus epididymidis

and analysis of glycerylphosphorylcholine, sialic acid and protein. J. Exp. Zool. 217:93-102.

(1981).

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15. Wang, C. Y., G. J. Killian and D. A. Chapman. Association of 14C-phosphatidylcholine

with epididymal sperm and its conversion to 14C-glycerylphosphorylcholine by sperm and principal

cells. Biol. Reprod. 25:969-976. (1981).

16. Killian, G. J. A rapid and simple method for isolating principal cells from the rat caput

epididymidis. J. Androl. 5:255-258. (1981).

17. Singleton, C. L. and G. J. Killian. A study of phospholipase in albumin and its role in

inducing the acrosome reaction of guinea pig spermatozoa in vitro. J. Androl. 4:150-156. (1983).

18. Miller, R. J. and G. J. Killian. Application of a FORTRAN computer program to quantify

rat epididymal tissue and cell alterations induced by basectomy. Acta Stereol. 2/Suppl. I-223:226.

(1983).

19. Miller, R. J., G. J. Killian and P. Vasilenko. Effects of long and short term vasectomy on

structural and functional parameters of the rat. J. Androl. 5:381-388. (1984).

20. Chapman, D. A. and G. J. Killian. Glycosidase activity in principal cells, basal cells,

fibroblasts and spermatozoa isolated from the rat epididymis. Biol. Reprod. 31:627-636. (1984).

21. Chapman, D. A., G. J. Killian, E. Gelerinter and M. T. Jarrett. Reduction of the spin label

TEMPONE by ubiquinol in the electron transport chain of intact rabbit spermatozoa. Biol. Reprod.

32:884-893. (1985).

22. Killian, G. J., E. Gelerinter and D. A. Chapman. Alterations of oxygen uptake and the redox

state of ubiquinone in rabbit sperm exposed to a variety of physiological treatments. Biol. Reprod.

33:859-869. (1985).

23. Hashlamoun, L. A. and G. J. Killian. Effects of timing of ovum recovery, cumulus cells,

sperm preincubation time, and pH on in vitro fertilization in C57BL/6 mice. Arch. Androl. 15:159-

171. (1985).

24. Hall, J. C. and G. J. Killian. Changes in rat sperm membrane glycosidase activities and

carbohydrate and protein contents associated with epididymal transit. Biol. Reprod. 36:709-718.

(1987).

25. Shabanowitz, R. B. and G. J. Killian. Two-dimensional electrophoresis of proteins in

principal cells, spermatozoa and fluid associated with the rat epididymis. Biol. Reprod. 36:753-768.

(1987).

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26. Miller, J. R. and G. J. Killian. Morphometric analysis of epididymides from normal and

vasectomized rats. J. Androl. 8:279-291. (1987).

27. Wang, C. Y. and G. J. Killian. Studies on the function of L-α-glycerylphosphorylcholine in

rat epididymal sperm and principal cells. Annals, N. Y. Acad. Sci. 513:549-550. (1987).

28. Honadel, T. E. and G. J. Killian. Cryopreservation of murine embryos with trehalose and

glycerol. Cryobiology 25:331-337. (1988).

29. Kavanaugh, J. F. and G. J. Killian. Bovine oviductal cannulations. J. Investigative Surgery

1:201-208. (1988).

30. Killian, G., Honadel, T., McNutt, T., Henault, M., Wegner, C., and D. Dunlap. Evaluation

of butylated hydroxytolune as a cryopreservative added to whole or skim milk diluent for bull

semen. J. Dairy Sci. 72:1291-1295. (1989).

31. Hall, J. C. and G. J. Killian. Two-dimensional gel electrophoretic analysis of rat sperm

membrane interaction with cauda epididymal fluid. J. Androl. 10:64-76. (1989).

32. Killian, G. J., D. A. Chapman, J. F. Kavanaugh, D. R. Deaver and H. B. Wiggin. Changes in

phospholipids, cholesterol and protein content of oviductal fluid of cows during the oestrous cycle.

J. Reprod. Fert. 86:419-426. (1989).

33. McNutt, T. L., G. J. Killian. Changes in guinea pig and bovine sperm protein carbohydrates

and glycosidase activities during in vitro capacitation. Molec. Androl. 1:67-86. (1989).

34. Henney, S. R., G. J. Killian and D. R. Deaver. Libido, hormone concentrations in blood

plasma and semen characteristics in Holstein bulls. J. Anim. Sci. 68:2784-2792. (1990).

35. Gerena, R. L. and G. J. Killian. Electrophoretic characterization of proteins in oviduct fluid

of cows during the estrous cycle. J. Exp. Zool. 256:113-120. (1990).

36. Page, K. C., G. J. Killian and S. E. Nyquist. Sertoli cell glycosylation patterns as affected by

culture age and extracellular matrix. Biol. Reprod. 43:659-664. (1990).

37. Dunlap, D. C. and G. J. Killian. Changes in phospholipids and phospholipase A2 activity of

guinea pig sperm membranes during in vitro capacitation. Mol. Androl. 3:1-17. (1991).

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38. McNutt, T. L. and G. J. Killian. Influence of bovine follicular and oviduct fluid on sperm

capacitation in vitro. J. Androl. 12:244-252. (1991).

39. Wegner, C. C. and G. J. Killian. In vitro and in vivo association of an oviduct estrus

associated protein with bovine zona pellucida. Mol. Reprod. Develop. 29:77-84. (1991).

40. Grippo, A. A., M. A. Henault, S. R. Henney and G. J. Killian. Cation concentration in fluid

from the oviduct ampulla and isthmus of cows during the estrous cycle. J. Dairy Sci. 75:58-65.

(1992).

41. Kavanaugh, J. K., A. A. Grippo and G. J. Killian. Cannulation of the bovine ampullary and

isthmic oviduct. J. Investigative Surgery. 5:11-17. (1992).

42. Wegner, C. C. and G. J. Killian. Origin of estrus-associated glycoproteins in bovine oviduct

fluid. J. Reprod. Fert. 95:841-854. (1992).

43. McNutt, T., L. Rogowski, R. Vasilatos-Younken and G. Killian. Uptake of oviductal fluid

proteins by the bovine sperm membrane during in vitro capacitation. Molec. Reprod. Develop.

33:313-323. (1992).

44. King, R. S., S. H. Anderson and G. J. Killian. Bovine estrus-associated protein facilitates

sperm capacitation and fertilizing ability. Proc. 12th Internat. Congr. on Anim. Reprod. 1:487-489.

(1992).

45. Killian, G. J. and A. A. Grippo. Incubation in ampullary oviductal fluid improves the ability

to sperm to fertilize bovine oocytes in vitro. Proc. 12th Internat. Congr. on Anim. Reprod. 2:650-

652. (1992).

46. Anderson, S. H., R. S. King and G. Killian. Effect of in vivo and in vitro derived bovine

estrus-associated protein on sperm capacitation, motility and agglutination. Proc. 12th Internat.

Congr. on Anim. Reprod. 1:396-398. (1992).

47. Henault, M. A. and G. J. Killian. Neutral lipid droplets in bovine oviductal epithelium and

lipid composition of epithelial cell homogenates. J. Dairy Sci. 76:691-700. (1993).

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48. Henault, M. A. and G. J. Killian. Synthesis and secretion of lipids by bovine oviduct

mucosal explants. J. Repro. Ferti. 98:431-438. (1993).

49. Henault, M. A. and G. J. Killian. Composition and morphology of lipid droplets from

oviduct epithelial cells. Anat. Rec. 237:466-474. (1993).

50. Killian, G. J., D. A. Chapman and L. A. Rogowski. Fertility associated proteins in Holstein

bull seminal plasma. Biol. Reprod. 49:1202-1207. (1993).

51. Yanagimachi, R., H. Katayose, G. Killian, C. N. Lee, D. T. Carrell and T. T. F. Huang.

Moderate heat treatment increases the penetrability of zona pellucida of salt-stored mammalian

oocytes by spermatozoa. Zygote 1:345-351. (1993).

52. Anderson, S., W. Harkness, Y. Akin, M. Kaproth and G. Killian. Categorical data analysis

of the effect of butylated hydroxytoluene as a cryoprotectant on bull fertility. J. Dairy Sci. 77:2302-

2307. (1994).

53. King, R. S. and G. J. Killian. Purification of bovine estrus-associated protein and

localization of binding on sperm. Biol. Reprod. 51:34-42. (1994).

54. McNutt, T. L., P. Olds-Clark, A. L. Way, S. S. Suarez and G. J. Killian. Effect of follicular

or oviductal fluids on movement characteristics of bovine sperm during capacitation in vitro. J.

Androl. 15:328-336. (1994).

55. King, R. S., S. H. Anderson and G. J. Killian. Effect of bovine oviductal estrus-associated

protein on the ability of sperm to capacitate and fertilize oocytes. J. Androl. 15:468-478. (1994).

56. Anderson, S. H. and G. J. Killian. Effect of macromolecules from oviductal conditioned

medium on bovine sperm motion and capacitation. Biol. Reprod. 51:795-799. (1994).

57. Skinner, S. M., G. J. Killian, L. A. Miller and B. S. Dunbar. Characterization of antigenicity

and immunogenicity patterns of native and recombinant zona pellucida (ZP) proteins in the white-

tailed deer (Oidocoileus virginianus). J. Reprod. Fert. 101:295303. (1994).

58. Grippo, A. A., S. H. Anderson, D. A. Chapman, M. A. Henault, and G. J. Killian.

Cholesterol phospholipid and phospholipase activity of ampullary and isthmic fluid from the bovine

oviduct. J. Reprod. Fert. 102:87-93. (1994).

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59. Grippo, A. A., A. L. Way and G. J. Killian. Effect of bovine ampullary and isthmic

oviductal fluid on motility, acrosome reaction and fertility of bull spermatozoa. J. Repord. Fert.

105:57-64. (1995)

60. Henault, M. A., G. J. Killian, J. F. Kavanaugh and L. C. Griel. Effect of accessory sex gland

fluid from bulls of differing fertilities on the ability of cauda epididymal sperm to penetrate zona-

free bovine oocytes. Biol. Reprod. 52:390-397. (1995).

61. Henault, M. A. and G. J. Killian. Effect of sperm preparation and bull fertility on in vitro

penetration of zona-gree bovine oocytes. Theriogenology 43:739-749. (1995).

62. Way, A. L., M. A. Henault and G. J. Killian. Comparison of four staining methods for

evaluating acrosome status and viability of ejaculated and cauda epididymal bull sperm.

Theriogenology 43:1301-1316. (1995).

63. Henault, M. A. and G. J. Killian. Effect of homologeous and heterologous seminal plasma

on the fertilizing ability of ejaculated bull spermatozoa assessed by penetration of zona-free bovine

oocytes. J. Reprod. Fert. 108:199-204. (1996).

64. Way, A. L., A. M. Schuler, and G. J. Killian. Influence of bovine ampullary and isthmic

oviductal fluid on bovine sperm-egg binding and fertilization in vitro. J. Reprod. Fert. 109:95-101.

(1997).

65. Cancel, A. M., D. A. Chapman, and G. J. Killian. Osteopontin is the 55-kilodalton fertility-

associated protein in Holstein bull seminal plasma. Biol. Reprod. 57:1293-1301. (1997).

66. Gerena, R. L., D. Irikura, Y. Urade, N. Eguchi, D. A. Chapman and G. J. Killian.

Identification of a fertility-associated protein in bull seminal plasma as lipocalin type prostaglandin

D synthase. Biol. Reprod. 58:826-833. (1998).

67. Staros, A. L. and G. J. Killian. In vitro association of six oviductal fluid proteins associated

with the bovine zona pellucida. J. Reprod. Fertil. 112:131-137. (1998).

68. Rodriguez, C. and G. Killian. Identification of ampullary and isthmic oviductal fluid

proteins that associate with the bovine sperm membrane. Anim. Reprod. Sci. 54:1-12. (1998).

69. Cancel, A. M., D. A. Chapman and G. J. Killian. Osteopontin localization in the Holstein

Bull Reproductive Tract. Biol. Reprod. 60:454-460. (1999).

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70. Topper, E. K., G. J. Killian, A. Way, B. Engel and H. Woelders. Influence of capacitation

and fluids from the male and female genital tract on the zona binding ability of bull spermatozoa. J.

Reprod. Fert. 115:175-183. (1999).

71. Miller, L. A., B. E. Johns, D. J. Elias and G. J. Killian. Oral vaccination of white-tailed deer

using a recombinant Bacillus Calmette-Guerin vaccine expressing the Borrelia burgdorferi outer

surface protein A: Prospects for immunocontraception. Amer. J. Reprod. Immunol. 41:279-285.

(1999).

72. Killian, G. J., Chapman, D. A., Cancel, A. M., Gerena, R. L., Rodriguez, C. M. and J. R.

Day. Male factors affecting sperm fertility. Rev. Bras. Reprod. Anim. 23:83-85. (1999).

73. Miller, L. A., Johns, B. E. and G. J. Killian. Long-term effects of PZP immunization on

reproduction in white-tailed deer. Vaccine 18:568-574. (2000).

74. Way, A. L., Griel, L. C. and G. J. Killian. Effects of accessory sex gland fluid on viability,

capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa. J. Androl. 21:213-

219. (2000).

75. Gerena, R. L., Irikura, D., Eguchi, N., Urade, Y. and G. J. Killian. Immunocytochemical

localization of lipocalin-type prostaglandin D synthase in the bull testis and epididymis and on

ejaculated sperm. Biol. Reprod. 62:547-556. (2000).

76. Rodriguez, C. M., Day, J. R. and G. J. Killian. Osteopontin gene expression in the Holstein

bull reproductive tract. J. Androl. 21:414-420. (2000).

77. Miller, L. A., Johns, B. E. and G. J. Killian. Immunocontraception of white-tailed deer

using native and recombinant zona pellucida vaccines. Animal Reprod. Sci. 63:187-195 (2000).

78. Gerena, R. L., Eguchi, N., Urade, Y. and G. J. Killian. Stage and region-specific localization

of lipocalin type prostaglandin D synthase in the adult murine testis and epididymis. J. Adrol.

21:848-854 (2000).

79. Miller, L. A., Johns, B. E. and G. J. Killian. Immunocontraception of white-tailed deer with

GnRH vaccine. Amer. J. Reprod. Immun. 44:266-274. (2000).

80. Rodriguez, C. M., Day, J. R. and G. J. Killian. Expression of the lipocalin-type

prostaglandin D synthase gene in the reproductive tracts of Holstein bulls. J. Reprod. Fert. 120:303-

309. (2000).

Dr. Gary Killian

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81. Wijayagunawardane, M. P. B., Miyamoto, A., Taquahashi, Y., Acosta, T. J., Gabler, C.,

Nishimura, M., Killian, G. and K. Sato. In vitro regulation of local secretion and contraction of the

bovine oviduct: Stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and

inhibition by oxytocin. J. Endocrinology 168:117-130 (2001).

82. Gabler, C., Killian, G. J., and R. Einspanier. Differential expression of extracellular matrix

components in the bovine oviduct during the oestrous cycle. Reproduction 122:121-130 (2001)

83. Miller, L., Crane, K., Gaddis, S., and Killian, G.J. Porcine Zona Pellucida

Immunocontraception: Long-term health effects on white-tailed deer. J. Wildl. Manag. 65L:941-

945.(2001).

84. Way, A.L., Barbato, G.F. and G. J. Killian. Identification of norepinephrine in bovine

oviductal fluid by high performance liquid chromatography. Life Sci. 2001 Dec 21;70:567-76.

(2001).

85. Killian, G.J. and L.A. Miller.. Behavioral Observations and Physiological Implications for

White-tailed Deer Treated with Two Different Immuocontraceptives. Proceedings of the Wildlife

Damage Management Conference 9:283-291.( 2001).

86. Miller, L.A. and G.J. Killian. 2001. Seven Years of White-tailed Deer Immunocontraceptive

Research at Penn State University: A Comparison of Two Vaccines. Proceedings of the Wildlife

Damage Management Conference 9:60-69. (2001).

87. Way, A. L. and Killian G. J. Capacitation and induction of the acrosome reaction in bull

spermatozoa with norepinephrine. J. Androl. 23:352-357. (2002).

88. Lazarus, M. , Mundy, C. J., Eguchi, N., Matsumoto, S., Killian, G. J., Kubata, B. K., and

Urade, Y. Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases

in male mouse reproductive organs. Endocrinology 143:2410-2419. (2002).

89. Killian, G. Estrogen-associated glycoproteins in oviduct secretions: structure and evidence

for a role in fertilization. Pp. 187-201. In “Introduction to Mammalian Reproduction” D. Tulsiani,

ed. Kluwer Acad. Pub. Boston. (2002).

90. Miller, L.A. and Killian, G.J. In search of the active PZP epitope in white-tailed deer

immunocontraception. Vaccine 20:2735-2742. (2002).

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91. Fagerstone, K. A., Coffey, M.A., Curtis, P.D., Dolbeer, R.A., Killian, G.J., Miller, L.A. and

Wilmot, L.M. Wildlife Fertility Control. Wildl. Soc. Tech. Rev. 02-2, 29 pp. (2002)

92. Lavery, K. Way, A. and Killian, G. Identification of haptoglobin in oviduct fluid and tissues

of the cow reproductive tract. Reproduction 125:837-846. 2003.

93. Taitzoglou, I. A., Chapman D. A. and G. J. Killian. Induction of the acrosome reaction in

bull spermatozoa with plasmin. Andrologia 35: 112-116. 2003

94. Wijayagunawardane, M. P. B, Gabler, C., Killian, G. and A. Miyamoto. Tumor Necrosis

Factor in the Bovine Oviduct during the Estrous Cycle: mRNA Expression and Affect on

Secretion of Prostaglandins, Endothelin-1 and Angiotensin II, Biol. Reprod.69: 1341-1346. 2003.

95. Gabler C, Chapman DA, and GJ Killian. Expression and localization of osteopontin and

integrins in the bovine oviduct during the estrous cycle. Reproduction 126: 721-729. (2003).

96. Miller, L.A., Rhyan, J. and Killian, G. Evaluation of GnRH contraceptive vaccine using

domestic swine as a model for feral hogs. Proceedings of the 10th

Wildlife Damage Management

Conference. (K. A. Fagerstone, G. W. Witmer, Eds.) pp. 120-127. (2003).

97. Killian, G., Miller, L, Rhyan, J., Dees, T. and Doten, H. Evaluation of GnRH contraceptive

vaccine in captive feral swine in Florida. Proceedings of the 10th

Wildlife Damage Management

Conference. (K. A. Fagerstone, G. W. Witmer, Eds.) pp. 128-133. (2003).

98. Lavery, K., Gabler, C. Day, J. and Killian, G. Expression of haptoglobin mRNA in the liver

and oviduct during the oestrous cycle of cows (Bos taurus). Anim. Reprod. Sci., 84:13-26. (2004)

99. Gabler, C., Plath-Gabler, A.,Killian, G.J., Berish, B. and Schams, D. Expression pattern of

fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) system members in

bovine corpus luteum endothelial cells during treatment with FGF-2, VEGF or oestradiol. Reprod.

Domest. Anim. 39: 321-327. (2004).

100. Killian, G. Evidence for the role of oviduct secretions in sperm function, fertilization and

embryo development. Anim. Reprod. Sci. 82-83:141-153. (2004).

Dr. Gary Killian

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101. Lazarus, M. Eguchi, N. Matsumoto, S. Nagata, N., Yano T., Killian, G.J. and Urade, Y.

Expression of microsomal prostaglandin E synthase-1 and cyclooxygenases in male monkey

reproductive organs. Prostaglandins, Leukotrienes and Essential Fatty Acids. 71: 233-240. (2004).

102. Killian, G., Miller, L., Diehl, N.K. Rhyan, J and Thain, D. Evaluation of three contraceptive

approaches for population control of wild horses. Proc. Vertebr. Pest Conf. 21:263-268. (2004).

103. Miller, L. A., Rhyan, J and G.J. Killian. GonaConTM

, a versatile GnRH Contraceptive for a

Large Variety of Pest Animal Problems. Proc. Vertebr. Pest Conf. 21:269-273. (2004).

104. Taitzoglou IA, Chapman DA, Zervos IA, Killian GJ. Effect of plasmin on movement

characteristics of ejaculated bull spermatozoa.

Theriogenology 62:553-61. (2004).

105. Bergqvist, A.S., Killian, G., Erikson, D., Hoshino, Y., Bage, R., Sato, E., Rodriguez-

Martinez, H. Detection of Fas ligand in the bovine oviduct. Anim Reprod Sci. 86:71-88. 2005

106. Killian, G., Wagner, D. and Miller, L. Observations on the Use of the GnRH Vaccine

GonaConTM

in Male White-tailed Deer (Odocoileus virginianus). Proceedings of the 11th

Wildlife

Damage Management Conference. (K. A. Fagerstone, G. W. Witmer, Eds.) pp. 256-263. 2005

107. Moura A.A., Koc H., Chapman D.A., Killian G.J. Identification of proteins in the accessory

sex gland fluid associated with fertility indexes of dairy bulls: a proteomic approach. J Androl.

27:175-185. 2005

108. Way, A.L and Killian, G.J. Sperm Binding, In vitro fertilization, and in vitro embryonic

development of bovine oocytes fertilized with spermatozoa incubated with norepinephrine. Anim.

Reprod. Sci. 96:1-9. 2006

109. Killian, G., Miller, L., Rhyan, J. and Doten, H. Immunocontraception of Florida Feral

Swine with a Single-Dose GnRH Vaccine. Amer. J. Reprod. Immunol. 55: 378-384. 2006.

110. Moura, A.A., Koc, H., Chapman, D.A., Killian, G.J. Proteins of the cauda epididymal fluid

associated with fertility of mature dairy bulls. J. Androl. 27:534-541. 2006.

111. Killian, G., Miller, L., Diehl, N.K. Rhyan, J. and Thain, D. Long-term efficacy of three

contraceptive approaches for population control of wild horses. Proc. Vertebr. Pest Conf. 22: 67-71.

2006.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15226011

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Dr. Gary Killian

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112. Killian, G., Eisemann, J., Wagner, D. Werner, J., Shaw D, Brooks, J. Jenny Fisher, J.,

Hattel, A. and Miller, L. Safety and toxicity evaluation of GonaConTM

immunocontraceptive

contraceptive vaccine in white-tailed Deer (Odocoileus virginianus). Proc. Vertebr. Pest Conf. 22:

82-87. 2006

113. Miller, L.A., Talwar, G.P. and Killian, G. Contraceptive effect of a recombinant GnRH

vaccine in adult female pigs. Proc. Vertebr. Pest Conf. 22:106-109. 2006.

114. Miller, L.A., Rhyan, J.C., Gionfriddo, J.P., Bynum, K.S., and Killian, G.J. GonaConTM

vaccine suppresses boar taint in male pigs: an alternative to surgical castration. Submitted to J.

Anim. Sci. Dec. 2005.

115. Moura, A.A., Chapman, D.A and Killian, G.J Proteins of the accessory sex glands associated

with the oocyte-penetrating capacity of cauda epididymal sperm from Holstein bulls of documented

fertility. Mol Reprod Dev. 74:214-222. 2007.

116. Goncalves, R.F. and Killian, G.J. Influence of arginine-glycine-aspartic acid (RGD),

integrins (alpha(V) and alpha(5)) and osteopontin on bovine sperm-egg binding, and fertilization in

vitro. Theriogenology. 67:468-474. 2007.

117. Taitzoglou, I.A., Kokoli, A.N. and Killian, G.J. Modifications of surface carbohydrates on

bovine spermatozoa mediated by oviductal fluid: a flow cytometric study using lectins.

Int J Androl. 2007. 30:108-14.

118. Brandt GA, Parks TE, Killian G, Ealy AD, Green JA A cloning and expression analysis of

pregnancy-associated glycoproteins expressed in trophoblasts of the white-tail deer placenta. Mol

Reprod Dev. 2007. 74:1355-62.

119. Moura AA, Chapman DA, Killian GJ A comprehensive proteomic analysis of the accessory

sex gland fluid from mature Holstein bulls. Anim Reprod Sci. 2007. 98:169-88.

120. Erikson DW, Way AL, Chapman DA, Killian GJ Detection of osteopontin on Holstein bull

spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine

fertilization. Reproduction. 2007.133:909-17.

121. Erikson, D.W., A.L. Way A.L., Bertolla, R.P., Chapman, D.A. and G.J. Killian. Influence of

osteopontin, casein and oviductal fluid on bovine sperm capacitation. Anim. Reprod. 2007. 4:1-3-

112.

122. Goncalves, R.F., Barnabe, V.H. and Killian, G.J. Pre-treatment of cattle sperm and/or oocyte

with antibody to lipocalin type prostaglandin D synthase inhibits in vitro fertilization and increases

sperm-oocyte binding. Anim Reprod Sci. 2008. 106:181-193.

123. Killian G., Fagerstone K., Kreeger T., Miller L., Rhyan J. Management strategies for

addressing wildlife disease transmission: Case for fertility control. Wildl Damage Manage Conf

2008; 12: 265-271.

124. Souza, C.E.A., Moura, A.A., Monaco, E. and Killian G.J. Binding patterns of bovine

seminal plasma proteins A1/A2, 30kDa and osteopontin on ejaculated sperm before and after

incubation with isthmic and ampullary oviductal fluid. Anim. Reprod. Sci. 2008. 105:72-89.

125. Goncalves, R.F., Chapman, D.A., Bertolla, R.P., Eder, I. and Killian, G.J. Pre-treatment of

cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo

development. Anim. Reprod. Sci. 2008. 108:375-383.

Dr. Gary Killian

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126. Miller, L. A., Gionfriddo, J.P., Rhyan, J.C., Fagerstone, K.A., Wagner, D.C. and Killian,

G.J. 2008. GnRH immunocontraception of male and female white-tailed deer fawns. Human-

Wildlife Conflicts 2:93-101.

127. Killian, G., Thain, D. Diehl, N.K., Rhyan, J., and Miller, L. 2008. Four-year contraception

rates of mares treated with single-injection porcine zona pellucida and GnRH vaccines and

intrauterine devices. Wildl. Res. 35: 531-539.

128. Killian, G. J., D. Thain, N. K. Diehl, J. C. Rhyan, and L. A. Miller. 2008. Four-year

contrception rates of mares treated with single-injection porcine zona pellucida and GnRH vaccines

and intrauterine devices. Wildlife Research 35:531-539.

129. Killian, G. J., D. Wagner, K. A. Fagerstone, and L. A. Miller. 2008. Long-term efficacy and

reproductive behavior associated with GonaContm

use in white-tailed deer (Odocoileus virginianus).

Proceedings of the Vertebrate Pest Conference 23:240-243.

130. Miller, L. A., K. A. Fagerstone, J. Kemp, and G. J. Killian. 2008. Immune mechanisms and

characterization of injection site reactions involved in the multi-year contraceptive effect of the

GonaContm

vaccine. Proceedings of the Vertebrate Pest Conference 23:244-249.

131. Miller, L. A., J.Gionfriddo, K. A. Fagerstone, J. Rhyan, and G. Killian. 2008. The single-

shot GnRH imunocontraceptive vaccine (GonaConTM

) in white-tailed deer: comparison of several

GnRH preparations. American Journal of Reproductive Immunology 60:214-223.

132. Gonçalves RF, Staros AL and Killian GJ. 2008. Oviductal fluid proteins associated with the

bovine zona pellucida and the effect on in vitro sperm-egg binding, fertilization and embryo

development. Reprod Domest Anim. 43:720-9.

133. Gonçalves RF, Chapman DA, Bertolla RP, Eder I and Killian GJ. 2008. Pre-treatment of

cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo

development. Anim Reprod Sci. 108:375-83.

134. Killian, G., T. J. Kreeger, J. Rhyan, K. Fagerstone, and L. Miller. 2009. Observations on the

use of Gonacontm

in captive female elk (Cervus elaphus). Journal of Wildlife Diseases 45:184-188.

135. Miller, L. A., K. A. Fagerstone, D. C. Wagner, and G. J. Killian. 2009. Factors contributing

to the success of a single-shot, multiyear PZP immunocontraceptive vaccine for white-tailed deer.

Human-Wildlife Conflicts 3:103-115.

136. Monaco E, Gasparrini B, Boccia L, De Rosa A, Attanasio L, Zicarelli L, Killian G. 2009.

Effect of osteopontin (OPN) on in vitro embryo development in cattle. Theriogenology. 71:450-7.

137. Gonçalves RF, Wolinetz CG, Barnabe VH, Killian GJ. 2009. Influence of osteopontin in

bovine uterine tube fluid on sperm binding and fertilization in RCA-1 lectin-treated oocytes. Reprod

Domest Anim. 44:152-5.

Technical Papers Published 1992-Present

1. Killian, G. 1992. Fertility factors in seminal plasma. In: Proceedings of the 14th

National

Association of Animal Breeder’s Technical Conference. P. 33-38.

2. Killian, G. 1992. How does the oviduct influence fertility? In: Proceedings of the 14th

National

Association of Animal Breeder’s Technical Conference. P. 104-108

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Dr. Gary Killian

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3. Allen, C. H., H. B. Wiggin and G. J. Killian. 1992. Experiences with paper bedding with bulls.

In: Proceedings of the 14th

National Association of Animal Breeder’s Technical Conference. P.

89-92.

4. Killian, G., D. Chapman, E. Cash, J. Griel, Jr., C. Allen, G. Gilvert, M. Kaptorth, R. Lenz, C.

Marshall. 1996. Conditions which affect the abundance of fertility-associated proteins in

seminal plasma. In: Proceedings of the 16th

National Association of Animal Breeder’s

Technical conference. P. 57-60.

5. Shumake, S., G. Killian. 1997. White-tailed deer activity, contraception, and estrous cycling.

In: 13th

Great Plains Wildlife Damage Control Workshop Proceedings. P. 124-131.

Published Abstracts 1973-Present

1. Killian, G. J. and R. P. Amann. Localization of neutral lipid and choline-phospholipid in the

epididymal epithelium of neopuberal Holstein bulls (Abstr.). Biol. Reprod. 9:90 (1973).

2. Killian, G. J. and R. P. Amann. Separation of cell types comprising the caput epididymidis

by sedimentation at unit gravity (Abstr.). Fed. Proc. 34:255 (1975).

3. Robb, G. W., G. J. Killian, R. P. Amann and W. W. Wilfinger. Reproductive development

of male rats: Effect CPZ (Abstr.). J. Anim. Sci. 42:1578 (1976).

4. Malinak, D. A. and G. J. Killian. Histochemical characterization of hydroxysteroid

dehydrogenases in testes and epididymides from adult rats and hamsters (Abstr.). Trans.

Penn. Acad. Sci. 50 (1977).

5. Killian, G. J. Separation of dispersed rat epididymal cells by centrifugal elutriation (Abstr.).

Fed. Proc. 37:380 (1978).

6. Maggio, M. J. and G. J. Killian. Electrophoresis of proteins from sperm and somatic cells

comprising the rat epididymis (Abstr.). Biol. Reprod., 20, Suppl. 1, 63A (1979).

7. Dorsey, W. F., D. A. Chapman, G. J. Killian. Carnitine content in rat epididymal sperm and

epithelial cells isolated from the caput and corpus epididymidis by centrifugal elutriation

(Abstr.). Biol. Reprod. Suppl. 1, 22:41A (1980).

8. Gelerinter, E., Z. Yaniv, G. Killian, L. Ritzert, D. Chapman, R. Chung. A study of the

interaction between a nitroxide spin probe (TEMPONE) and rabbit spermatozoa (Abstr.).

Bull Am. Physical Soc. 26:247 (1981).

9. Singleton, C. L. and G. J. Killian. Phospholipase A2 in albumin induces the acrosome

reaction in vitro of guinea pig sperm (Abstr.). Physiologist 24:45 (1981).

Dr. Gary Killian

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10. Miller, R. J. and G. J. Killian. A morphometric system employing stereologic techniques

and FORTRAN computer programming for quantifying cellular dynamics in the rat

epididymis (Abstr.). Ohio J. Sci. 81:72 (1981).

11. Killian, G. J. and D. A. Chapman. Differences in glycosidase activity among cell types

comprising the rat epididymis (Abstr.). J. Andrology 3:30 (1982).

12. Miller, R. J. and G. J. Killian. Effects of short- and long-term vasectomy of anatomic,

physiologic, immunologic and hematologic parameters of rats (Abstr.). J. Andrology 3:37

(1982).

13. Wang, C. Y. and D. A. Chapman and G. J. Killian. Synthesis of glycerylphosphorylcholine

(GPC) from phosphatidylcholine (PTC) in vitro by rat epididymal sperm and principal cells

(Abstr.). J. Andrology 3:29 (1982).

14. Miller, R. J., J. M. Purtillo and G. J. Killian. A user simplified FORTRAN computer

program for stereological analysis of complex multicellular organs (Abstr.). Trans. Amer.

Micro. Soc. 101:113 (1982).

15. Shabanowitz, R. K. and G. J. Killian. Isoelectric focusing and two-dimensional

electrophoresis of caudal epididymal fluid of the rat (Abstr.). Ohio J. Sci. 82:6 (1982).

16. Miller, R. J. and G. J. Killian. Rat epididymal halo cells have lymphocytic characteristics

(Abstr.). Fed. Proc. (1983).

17. Shabanowitz, R. and G. J. Killian. Electrophoretic characterizations of cytoplasmic proteins

from the caput and corpus principal cells of the rat epididymis (Abstr.). Fed. Proc. (1983).

18. Miller, R. J., P. Vasilenko, C. Y. Wang, W. F. Dorsey and G. J. Killian. Age dependent

effect of hemicastration on male rat reproductive capacity (Abstr.). J. Andrology 4:38

(1983).

19. Gelerinter, E., D. A. Chapman, G. J. Killian and M. Jarrett. Reduction of the spin probe

TEMPONE by live rabbit sperm (Abstr.). Bull. Amer. Phys. Soc. 29:366 (1984).

20. Killian, G. J., D. A. Chapman and E. Gelerinter. ESR studies on electron flow through

ubiquinone in epididymal, ejaculated, capacitated and decapacitated rabbit spermatozoa

(Abstr.). Biol. Reprod., Suppl. 1 30:94 (1984).

21. Shabanowitz, R. B. and G. J. Killian. Loss of 20,000 dalton protein from cauda epididymal

sperm following thirty-six hours of efferent duct ligation (Abstr.). Biol. Reprod. Suppl. 1

32:126 (1985).

22. Miller, R. J. and G. J. Killian. Regional analyses of rat epididymal cells. Trans. Amer.

Microscopical Soc. 105:93 (1986).

Dr. Gary Killian

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23. McNutt, T. L. and G. J. Killian. Changes in membrane protein, carbohydrate and

glycosidase activities during in vitro capacitation of guinea pig sperm. (Abstr.). Biol.

Reprod. Suppl. 1, 34:93 (1986).

24. Dunlap, D. C. and G. J. Killian. Changes in phospholipase activity and phospholipid

composition of guinea pig spermatozoal membranes isolated during in vitro capacitation

(Abstr.). Biol. Reprod. Suppl. 1, 34:92 (1986).

25. Wegner, C., G. Killian and D. Chapman. Oxygen consumption rate varies with the

concentration of bull spermatozoa (Abstr.). J. Androl. 8:p-42 (1987).

26. Henault, M., G. Killian and D. Chapman. Changes in cytosolic protein and phospholipase

activity during in vitro capacitation of sperm from fertile and subfertile bulls (Abstr.). J.

Androl. 8:p-41 (1987).

27. McNutt, T., G. Killian and D. Chapman. Glycosidase activities in bovine sperm during in

vitro capacitation and the acrosome reactions (Abstr.). J. Androl. 8:p-41 (1987).

28. Killian, G. J., D. A. Chapman, J. Kavanaugh and D. R. Deaver. Changes in the lipid

composition of oviductal fluid during the cow estrous cycle (Abstr.). Biol. Reprod. Suppl. 1,

36, 84 (1987).

29. Kavanaugh, J. K. and G. J. Killian. Bovine oviductal cannulations (Abstr.). Investigative

Surgery (1988).

30. Killian, G., Honadel, T., McNutt, T., Henault, M., Wegner, C. and D. Dunlap. Evaluation

of butylated hydroxytoluene (BHT) in whole milk diluent for the cryopreservation of bull

semen. Proc. 12th Ann. Tech. Conf. AI and Reproduction (1988).

31. Gerena, R. L. and G. J. Killian. Estrus-associated 98 kDa protein in bovine oviductal fluid

(ODF). Biol. Reprod., Suppl. 1, 38:130 (1988).

32. Page, K. C., Nyquist, S. E. and G. J. Killian. Effects of culture age and substratum on sertoli

cell glycopeptide profiles. Biol. Reprod. Suppl 1, 38:88 (1988).

33. McNutt, T. and G. Killian. Effect of bovine oviduct fluid (ODF) on sperm capacitation in

vitro. J. Androl. 10:22-P. (1989).

34. Henney, S. R., Killian, G. J. and D. R. Deaver. Estradiol concentration in seminal plasma is

negatively correlated with sperm concentration in Holstein bulls. J. Androl. 10:24-P.

(1989).

35. Grippo, A. A., Chapman, D. A. and G. J. Killian. An improved assay for phospholipase A2

activity in sperm. J. Androl. 10:P47. (1989).

Dr. Gary Killian

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36. Henault, M. A. and G. J. Killian. Neutral lipid content of the oviduct epithelium varies with

region and the estrous cycle. (Abstr.). P. 428 In: "Fertilization in Mammals". B. Bavister,

J. Cummins, E. Roldan, eds. Serono Symposia, USA. Norwell, MA (1990).

37. McNutt, T. L. and G. J. Killian. Influence of bovine follicular fluid on sperm capacitation in

vitro. (Abstr.). P. 430 In: "Fertilization in Mammals". B. Bavister, J. Cummins, E. Roldan,

eds. Serono Symposia, USA. Norwell, MA (1990)

38. Wegner, C. C. and G. J. Killian. Estrus-Associated protein in synthesized by the bovine

oviduct. (Abstr.). P. 436 In: "Fertilization in Mammals" B. Bavister, J. Cummins, E.

Roldan, eds. Serono Symposia, USA. Norwell, MA (1990)

39. King, R. S., McNutt, T. L., Henault, M. A. and G. J. Killian. Effect of cumulus oophorus

and follicular cells on meiotic maturation in bovine oocytes. Biol. Reprod. Suppl. 1. 42:91.

(1990)

40. Ravnik, S. E., Killian, G. J. and C. H. Muller. Bovine reproductive fluids have lipid transfer

protein and stimulate human sperm capacitation. Biol. Reprod. Suppl. 1, 42:86 (1990).

41. King, R. S., Grippo, A.A. and G. J. Killian. Bovine estrus-associated protein stimulates

capacitation of bull sperm. Biol. Reprod. Suppl. 1, 44:135 (1991).

42. Henault, M. A. and G. J. Killian. Synthesis of lipids by bovine oviductal explants. Biol.

Reprod. Suppl. 1, 44:161 (1991).

43. McNutt, T., Rogowski, L. and G. Killian. Uptake of oviduct fluid (ODF) proteins by bovine

sperm membrane during in vitro capacitation. J. Androl. 12:41 (1991).

44. King, R. S., S. H. Anderson, and G. J. Killian. In vitro synthesized bovine estrus-associated

protein capacitates bull sperm. (Abstr.). J. Androl. Suppl. 13:P-24 (1992).

45. Anderson, S. H. and G. J. Killian. Sperm enhance the secretion of a 35-40 kDa and

oviductal protein during co-culture. (Abstr.). J. Androl. Suppl. 13:P-40 (1992).

46. Henault, M. A., A. L. Way, A. A. Grippo and G. J. Killian. A heterospermic competitive

penetration assay utilizing zona-free bovine oocytes. Biol. Reprod. Suppl. 1, 46:69 (1992).

47. Anderson S. H. and G. J. Killian. Bovine sperm alter the electrophoretic pattern of proteins

produced by ampullar oviductal explants. Biol. Reprod. Suppl. 1, 46:75 (1992).

48. Way A. L. and M. A. Henault. Comparison of staining techniques used for analysis of

bovine sperm viability and acrosomal integrity. Biol. Reprod. Suppl. 1, 46:93 (1992).

49. Anderson, S. H. and G. J. Killian. Detection of a 48 kDa, methionine-enriched oviductal

protein in conditioned media from bovine isthmic explants, but not ampullar explants. J.

Anim. Sci. Suppl. 1, 70:256 (1992).

Dr. Gary Killian

22

50. Taft, R., R. King, S. Anderson and G. Killian. In vitro capacitation of sperm from fertile and

subfertile bulls using oviductal fluid. J. Anim. Sci. Suppl. 1, 70256 (1992).

51. Way, A. L., M. A. Henault and G. J. Killian. Incubating bovine cauda epididymal sperm

(CES) in accessory gland fluid (AGF) affects sperm viability. J. Anim. Sci. Suppl. 1, 70:257

(1992).

52. Henault, M. A., A. L. Way and G. J. Killian. A sperm penetration assay utilizing zona-free

bovine oocytes. J. Anim. Sci. Suppl. 1, 70:257 (1992).

53. Henault, M. A. and G. J. Killian. Morphological characterization of bovine oviduct

epithelial cell lipid inclusions. Molec. Biol. Cell Suppl. Vol. 3 Abstract #1605. (1992).

54. Henault, M. A. and G. J. Killian. Positive effect of accessory gland fluid (AGF) on fertility

of bovine cauda epididymal sperm. J. Androl. 14:44. (1993).

55. Way, A. L., M. A. Henault and G. J. Killian. Evaluation of two dual acrosome and viability

stains for bovine cauda epididymal sperm. J. Androl. 14:53. (1993).

56. King, R. S., D. J. Zaczek and G. J. Killian. Localization of bovine estrus-associated protein

on sperm. Biol. Reprod. Suppl. 1, 49:116. (1993).

57. Killian, G. and D. Chapman. Fertility associated proteins in bull seminal plasma. Biol.

Reprod. Suppl. 1, 49:146. (1993).

58. Anderson, S. H. and G. J. Killian. Oviductal conditioned medium macromolecules affect

sperm capacitation. J. Androl. 15:22. (1994).

59. Cancel, A., D. A. Chapman and G. J. Killian. Isolation and detection of a 55 kDa fertility-

associated protein in bovine seminal plasma components. Biol. Reprod. Suppl. 1, 50:123.

(1994).

60. Cancel, A. and G. J. Killian. Osteopontin is the 55 kDa fertility-associated protein in bovine

seminal plasma. J. Androl. Suppl. 1, 16:39. (1995).

61. Rodriguez, C. M. and G. J. Killian. Effect of estrous cycle stages and region of the oviduct

on oviduct fluid proteins that associate with the sperm membrane. Biol. Reprod. Suppl. 1,

52:117. (1995).

62. Staros, A. L. and G. J. Killian. Six oviductal fluid proteins (ODF) associate with the bovine

zona pellucida in vitro. Biol. Reprod. Suppl. 1, 52:117. (1995).

63. Xia, P., G. J. Killian. Ampullar and isthmic oviduct fluid (ODF) have different effects on

gametes and fertilization in vitro. Biol. Reprod. Suppl. 1,52:123. (1995).

Dr. Gary Killian

23

64. Gerena R., D. A. Chapman and G. J. Killian. Identification of prostaglandin D synthase in

bovine sperm membranes, seminal plasma and tissue homogenates. Biol. Reprod. Suppl. 1,

54:219. (1996).

65. Killian, G., R. Gerena, D. Chapman and Y. Cao. A fertility-associated protein in bull

seminal plasma is prostaglandin D2 synthase. Prostaglandins 51:296. (1996).

66. Staros, A. L. and G. J. Killian. Improvement of in vitro fertilization rates following

treatment of oocytes with bovine estrus-associated protein (EAP). Biol. Reprod. Suppl. 1,

56:86. (1997).

67. Way, A. L., Zaczek, D. J. and G. J. Killian. Presence of a haptoglobulin-like protein in

bovine oviductal fluid (ODF). Biol. Reprod. Suppl. 1, 56:134. (1997).

68. .Xie, X. and G. J. Killian. Identification and characteristics of a 34 kDa bovine oviduct

specific protein. Biol. Reprod. Suppl. 1, 56:134. (1997).

69. Rodriguez, C. M., Gerena, R. L., Day, J. R., and G. J. Killian, Prostaglandin D synthase

mRNA and protein expression in the bull testis and epididymis. Biol. Reprod. Suppl. 1,

58:355. (1998).

70. Way, A. L., Hoffman, H. L., Shope, N.R., Miller, M. R. and G. J. Killian, Immunodetection

of a 40 kDa haptoglovin-like protein in bovine oviductal fluid (ODF) and reproductive tract

tissues. Biol. Reprod. Suppl. 1, 58:48 (1998).

71. Gabler C., Plath-Gabler, A., Killian, G. J., Spanel-Borowski, K. and D. Schams. Regulation

of angiogenic factors in bovine endothelial cells of the corpus luteum by estradiol, vascular

endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2). Exp. Clin.

Endocrinol Diabetes Suppl. 1, 107:S30 (1999)

72. Vieira, E. G., Chapman, D. A. and G. J. Killian. Evidence for multiple forms of estrus-

associated protein in bovine oviductal fluid associated with oviduct region and stage of the

estrous cycle. Biol. Reprod. Suppl. 1, 60:351 (1999).

73. Gabler, Christoph, Chapman, D. A., Gerena, R. L., Sorensen, E. S. and G. J. Killian.

Osteopontin is present in the bovine oviduct epithelium and luminal fluid. Biol. Reprod.

Suppl. 1, 60:186. (1999).

74. Way, A. L., Miller, L. L., Dunbar, B. S., Hagen, D. R. and G. J. Killian. In vitro sperm-egg

binding assay for use in evaluating efficacy of different zona pellucida (ZP) immunogens for

contraception in white-tailed deer (Odecoileus Virginianus). Biol. Reprod. Suppl. 1, 60:432

(1999).

75. Gabler, C., Einspanier, R., Schams, D. and G. J. Killian. Regulation of the insulin-like

growth factor (IGF) system in the bovine oviduct. Reprod. Dom. 35:14 (2000).

Dr. Gary Killian

24

76. Way, A. L. and G. J. Killian. In vitro capacitation and acrosome reaction of bovine sperm

facilitated by norepinephrine (NE). J. Androl. Suppl. 1, 62:47 (2000).

77. Gabler C., Lavery, K. and G. J. Killian. Haptoglobin is expressed in bovine reproductive

tissues. Biol. Reprod. Suppl. 1, 62:262 (2000).

78. Lavery, K., Daniel, S., and G. Killian. Immunohistochemical localization of a bovine

haptoglobin-like protein in the female reproductive tract. Biol. Reprod. Suppl. 1, 62:344

(2000).

79. Killian, G., Chapman, D., Cancel, A. and R. Gerena. Protein markers for fertility in seminal

plasma. Proceedings of the 14th

International congress on Animal Reproduction, Vol.

2:W8:5 (2000).

80. Way, A. L. and Killian, G.J. Investigation into the existence of adrenergic receptors on bull

sperm. Biol. Reprod. 64, Suppl. 1: 297. (2001).

81. Golash, C.D. and Killian, G.J. Effect of staged oviductal fluid on lectin receptor sites of the

bovine zona pellucida. Biol. Reprod. 64, Suppl. 1: 318.( 2001).

82. Goncalves, R. F. Way, A.L. and Killian, G.J. Influence of osteopontin and lipocalin-type

prostaglandin D synthase in sperm binding and fertilization in vitro. Biol. Reprod. 64, Suppl.

1: 111-112. ( 2001).

83. Galantino-Homer, H. Megee, S.O., Killian, G.J. Kopf, G.S. and Dobrinski, I. Effecrt of the

oviduct on bovine sperm capacitation and protein phosphorylation. Biol. Reprod. 64, Suppl.

1: 274-275. (2001).

84. Bergqvist, A-S. I. , Killian, G. and Rodriguez-Martinez, H. FAS ligand in the bovine oviduct

may contribute to its immune-privilaged status. Biol. Reprod. Suppl. 1, 66:315.(2002).

85. Golash, C. D., Phillips, A. T. and Killian, G. J. Fucosyltransferase and galactosyltransferase

activities in bovine oviductal fluid: variation with the estrous cycle and in vitro sperm

binding and embryo effects. Reprod. Suppl. 1, 66:317. (2002).

86. Killian, G.J. and Goncalves, R.F. Negative affect of antibodies to bovine osteopontin,

lipocalin prostaglandin D synthase and serum albumin on in vitro embryo development of

oocytes exposed to oviduct fluid. Theriogenology 57:520. (2002).

87. Way, A., Goncalves, R.F. and Killian, G.J. Influence of osteopontin, lipocalin prostaglandin

D synthase and bovine serum albumin on sperm-oocyte binding and in vitro fertilization.

Theriogenology 57:669.(2002).

88. Erikson, D., Golash, C. and Killian, G.J. Increased binding of the oviductal glycoprotein

osteopontin, following treatment with RCA-1 lectin. Biol. Reprod. Suppl. 1, 66:168. (2002)

Dr. Gary Killian

25

89. Wijayagunawardane, M.P.B., Gabler, C. Killian, G. and Miyamoto, A. Tumor necrosis

factor in the bovine oviduct during the estrous cycle: mRNA expression and effect on

secretion of prostaglandins, endothelin-1 and angiorensin II. Biol. Reprod. Suppl. 1, 68:266.

(2003).

90. Goncalves, R. F. Chapman D.A. and Killian, G.J. Effect of osteopontin on in vitro bovine

embryo development. Biol. Reprod. Suppl. 1, 68:336. (2003).

91. Erikson, D.W., Chapman, D.A., Ealy, A.D. and Killian, G.J. Immunodetection of

osteopontin on Holstein bull sperm and of αV and α5 integrins on bovine oocytes. Biol.

Reprod. Suppl. 1, 68:349. (2003).

92. Erikson, D. W. and Killian, G. J. Evidence for binding of osteopontin from oviductal fluid to

integrins on sperm plasma membranes. Abstract presentation, 15th International Congress on

Animal Reproduction(ICAR). Porto Segoro, BA, Brazil. August 1-5, 2004. ICAR

Proceedings 1:1. (2004).

93. Gonçalves, R.F., Erickson, D., Ealy, A.D., Killian, G.J. Influence of argenine-glycine-

aspartic acid (RGD) in bovine sperm-egg binding, and fertilization in vitro Reprod. Fert.

Develop 16:256. (2004).

94. Gonçalves, R. F., Garcia, J. M., Tomita, S. Y. and Killian, G. J. Pretreatment of bovine

sperm or ova with antibody to osteopontin inhibits fertilization. Abstract presentation, 15th

International Congress on Animal Reproduction. Porto Segoro, BA, Brazil. August 1-5,

2004. ICAR Proceedings 1:232. (2004).

95. Souza, C.E.A., Radis-Batista,G , Oliveira,J.T.A, Araujo,A.A. Lima, A.C.B.,Killan, G.J. and

Moura, A.A. Osteopontin and 17b secretion in seminal plasma of Santa Ines hairy rams.

Abstract presentation, 15th International Congress on Animal Reproduction. Porto

Segoro,BA, Brazil. August 1-5, 2004. ICAR Proceedings 1:202. (2004).

96. Moura, A.A., Chapman, D.A. and Killian, G.J. Proteins in the accessory gland and

epididymal fluid as related to dairy bull fertility. J. Androl. Suppl. 26:132. (2005).

97. Moura, A. A., Chapman, D. A. and Killian, G. J. Identification of proteins from accessory

gland fluid the influence the oocyte penetrating ability of bovine cauda epididymal sperm in

vitro, Biol. Reprod Suppl. 73:86. (2005).

98. Erikson, D.W. and Killian, G.J. Localization of osteopontin on ejaculated and epididymal

Holstein sperm and flow cytometric analysis of osteopontin on ejaculated Holstein bull

sperm. Biol. Reprod. Suppl. 73:W528. (2005).

99. Goncalves, R. and Killian, G. Influence of integrins αV and α5 in bovine sperm-egg binding

and fertilization in vitro. Biol. Reprod. 73:T32. (2005).

100. Moura, A.A., Chapman, D.A., Koc, H. and Killian, G.J. A comprehensive proteomic

analysis of cauda epididymal fluid and identification of proteins associated with fertility

Dr. Gary Killian

26

scores of mature Holstein bulls. J. Androl. Suppl. 27:77. (2006).

Patents

PSU Inv. Disc. No. 93-1274

U.S. Patent No. 5,569,581 (November 1996)

Killian, G., D. Chapman, A. Cancel and M. Henault;

Alteration and Prediction of Male Fertility Using Seminal Plasma and Its Components

Dr. Gary Killian

27

Funding

Date

Title

Submitted To Amount

Funded

Indirect

1974-76

Separation and Culture of

Epididymal Epithelial Cells (HD-

07244)

NIH

$103,058

1975-76

Male Reproductive Physiology

Research Initiation Grant

Program (PSU)

$4,242

1982-85

ESR Studies on Sperm Capacitation

(HD13422)Last year at PSU

NIH

$256,411

1985-86

Studies on the Role of Epididymal

Glycosidases in Modifying Sperm

Membrane Glycoproteins

Biomedical Research

Support Grant Program

(PSU)

$5,500

1985-86

Evaluation of Butylated

Hydroxytoluene as a

Cryopreservative for Freezing Bull

Semen

Eastern Artificial

Insemination Cooperative

$4,000

1985-87

Protein Analyses of Bull Sperm

Membranes and Seminal Plasma

Sire Power, Inc.

$12,000

1987-88

Studies on Semen Physiology and

Sexual Behavior of Bulls

Atlantic Breeders

Cooperative

$6,000

1987-88

Evaluation of Antioxidants in the

Preservation of Bull Semen

Eastern Artificial

Insemination Coop.

$4,000

1985-89

Bovine Oviductal Fluid Biochemistry

and its Relationship to Gamete

Physiology (85-CRCR-1-1841)

USDA Competitive Grant

Program

$215,850

1988-89

Evaluation of Antioxidants and

Polymeric Compounds in the

Cryopreservation of Bull Semen

Eastern Artificial

Insemination Cooperative

$4,000

1986-87

Eval.of Trehalose as a Cryoprotectant

for Freezing Bull Semen

Atlantic Breeders

Cooperative

$6,000

1988

Electron Paramagnetic Resonance

Spectrometer. Co-PI with R.

Hammerstedt, (RR04232)

NIH

$188,000

1988-89

Evaluation of the Relationship

Between Bull Libido and Blood

Atlantic Breeders

Cooperative

$5,000

Dr. Gary Killian

28

Date

Title

Submitted To

Amount

Funded

Indirect

Hormone Concentrations 1986-90

ESR Studies on Sperm Capacitation

(HD20272)

NIH

$338,482

1987-92

Food and Agricultural Sciences

National Needs Graduate

Fellowships Co-PI with Craig

Baumrucker, (#87-GRAD-9-0082)

USDA

$96,000

1989-91

Early Detection of Subfertility in

Holstein Bulls

Atlantic Breeders, Eastern

AI, Sire Power

$39,000

1989-92

Effect of Oviduct Fluid Composition

on Sperm Motility and Capacitation.

(89-372-4771)

USDA-NRI Competitive

Grant

$179,000

1991-92

Diagnostic Test for Subfertility in

Bulls

(92C. 1023R2)

Ben Franklin Atlantic

Breeders

Sire Power

$30,000

1990-93

Male Factors Affecting Fertility of

Bovine Sperm (90-37240-5512)


Grant

$142,664 $19,973

1991-92

Effect of Feeding Commingled

Recyclable Paper on Semen

Characteristics of Holstein Bulls

Atlantic Breeders

Cooperative

$13,000

1991-95


on Sperm Motility and Capacitation

(91-37203-6554)


Grant

$258,200 $36,148

1992

Immunocontraception of Deer, Co-

PI, D. Hagen (#12-34-74-0218)

USDA-APHIS

$71,220 $7,122

1993

Immunocontraception of Deer, Co-

PI, D. Hagen (#12-34-74-0218)

USDA-APHIS

$69,439 $6,944

1992-93

Diagnostic Test for Subfertility in

Bulls

Ben Franklin, Atlantic

Breeder, Sire Power

$30,000 $6,450

1993

Post-doctoral Training Support for

Dr. Omar Salama (6 months)

Egyptian National

Research Project (USAID)

$6,025 $2,591

1993

Post-doctoral Training Support for

Arab Republic of Egypt

$5,650 $2,429

Dr. Gary Killian

29

Date

Title

Submitted To

Amount

Funded

Indirect

Dr. Omar Salama (6 months)

1993 Post-doctoral training support for Dr.

Mohamed Gabr

Egyptian National


$6,025 $2,591

1993

Technical Exchange, Dr. E. Shehata

Egyptian National


$4,600

1993 Technical Training for Ms. Doaa

Teleb

Egyptian National


$8,579

1993 Travel grant for G. Killian to go to

Egypt

College of Ag. Sciences $5,000

1993-

1997


Bovine Sperm (93-37203-9069)

USDA-NRI

Competitive Grant

$212,000 $29,680

1994 Development of a Diagnostic Test for

Predicting Bull Feritlity

A.I. Cooperatives

Consortium

$49,000

1994 Immunocontraception of Deer, Co-

PI, D. Hagen

USDA-APHIS $83,643 $8,389

1995 Immunocontraception of Deer, Co

PI-D. Hagen

USDA-APHIS $77,753 $7,775

1996 Studies on the Bovine Oviduct

Holland Genetics

$22,550

$0

1996 Improved Technology for Bovine

Embryo Production

Ben Franklin Challenge

Grant $52,284 $0

1996 Immunocontraception of Deer, Co P

I-D. Hagen USDA-APHIS $77,753 $7,775

1996

Improved Technology for Bovine

Embryo Production

EM TRAN, Inc.

$52,284

$22,482

1996-

1999


on Sperm Motility and Capacitation

USDA-NRI

Competitive Grant

$194,462 $27,225

1997 Immunocontraception of Deer USDA-APHIS

Cooperative Agreement

$82,488 $8,249

1997 Improved Technology for Bovine

Embryo Production

Ben Franklin Challenge

Grant

$61,643 $0

Dr. Gary Killian

30

Date

Title

Submitted To

Amount

Funded

Indirect

1997 Development of a Diagnostic Test for

Predicting Bull Fertility

Genex $20,000 $0

1997-

2000


Bovine Sperm

USDA-NRI

Competitive Grant

$250,000 $35,000

1998

Support for Studies on the Bovine

Oviduct

Holland Genetics

$10,000

$0

1998

Diagnostic Tests Predicting Fertility

Genex.

$20,000

$0

1998

Immunocontraception of Deer

USDA-APHIS


$73,096

$7,310



$79,808 $7,981

2000 Dairy Profitability Training for Baltic

Specialist II Co-PI with Don Evenson

USDA Cooperative

Agreement

$48,674 $4,867



$10,687 $1,069

Dr. Gary Killian

31



$78,329 $7,833

2001 Contraception of Feral Swine USDA-APHIS


$75,000 $7,500



$40,000 $4000

2001 Improved Fertility of Dairy Cattle USDA Special Grant $78,000 $7,800



$45,000 $4500

2002 Contraception of Feral Swine USDA-APHIS


$75,000 $7,500

2002 Improved Fertility of Dairy

Cattle(with A. Ealy)

USDA Special Grant $65,000 $6,500

2003 Technology to Improve Fertility of

Dairy Cattle


2003 Immunocontraception of White-

tailed Deer

USDA-APHIS


$20,000 $2000



$45,000 $4500

3/1/04-

2/28/05

Immunocontraception of Deer USDA-APHIS


$48,600 $4,860

9/1/04-

4/30/05

Target Safety trial testing the GnRH

Vaccine in Deer.

USDA-APHIS


$39,000 $3,900

10/1/04-

9/30/05

Evaluation of PZP

immunocontraceptive vaccine in deer

USDA-APHIS


$7,118 $711

9/1/04-

9/30/07

Improving reproductive efficiency of

dairy cattle G. Killian, PI, R.

Ramachandran and S. Stefanou, are

Co-I


3/01/06-

9/30/06

Immunocontraception of swine

comparing two GnRH vaccines

USDA-APHIS


$27,478 2,748

9/30/06- Immunocontraception of deer. USDA-APHIS $18,000 1,800

Dr. Gary Killian

32

9/29/07


Summary of funding by Year (1993-2007)

Year # Grant Application # Grants Funded $ Funded $ Indirect

1993 9 9 $489,982 $76,325

1994 2 2 $132,643 $8,364

1995 5 2 $342,092 $44,537

1996 4 4 $399,333 $57,402

1997 5 5 $484,774 $52,779

1998 3 3 $103,096 $7,310

1999 2 1 $79,808 $7,981

2000 4 3 $137,690 $13,769

2001 4 3 $193,000 $19,300

2002 3 3 $185,000 $18,500

2003 3 3 $181,000 $18,100

2004 4 4 $519,818 $51,982

2005 1 0 0

2006 3 2 $27,478 $2,748

2007 1 1 $18,000 $1,800

Graduate Theses Supervised, Type of Degree, Year Granted, Status of Student

Student Degree/Major

Year

Title

Present Position

George

William

Robb

M.S.

Dairy Science

Penn State

1976

Reproductive Development

of Wistar Rats With and

Without Postnatal

Chlorpromazine Treatment

Faculty member/

Cooperative

Extension

Michigan State

University William

Robert

Kemp

M.S.

Physiology

Penn State

1977

Glycosidase Activity in

Epithelial Cells Isolated

From Caput Epididymidis of

Chlordrydrin Treated Wister

Rats

Medical doctor

working in Lancaster,

PA.

David Scott

Hoffman

M.S.

Biological

Sciences

Kent State

1980 Separation of Epithelial Cells

From the Rat Corpus

Epididymidis and Analysis

of

Glycerylphosphorylcholine,

Lawyer in Akron,

OH.

Dr. Gary Killian

33

Student

Degree/Major

Year

Title

Present Position

Sialic Acid and Total Protein

Crystal

Singleton

M.S.

Biological

Sciences

1981 A Study of the Role of

Albumin in Guinea Pig

Sperm Capacitation

Physician in Ft.

Valley, Georgia

Janice A.

Granieri

Honors Thesis

Biological

Sciences

Kent State

1982 The Effect of Relaxin on

Glycogen and Protein

Content of the Reproductive

Tract in the Male Rat

Unknown

L. Jean

Patterson

Honors Thesis

Biological

Sciences

Kent State

1982 Effect of Relaxin in Sialic

Acid and

Glycerylphosphorylcholine

Levels in the Reproductive

System of the Male Rat.

Unknown.

Roman J.

Miller

Ph.D.

Physiology

Kent State

1983 The Rat Epididymis:

Morphometric Parameters,

Consequences of Vasectomy

and Halo Cell Characteristics

Professor - Holds the

Suter Endowed Chair

in the Biology Dept.

at Eastern Mennonite

College,

Harrisonburg, VA.

Linda Ann

Hoshlamoun

M.S.

Biological

Science

Kent State

1984 A Study to Define the

Optimum Conditions for In

Vitro Fertilization of

C57BL/6 Mouse Eggs.

Employed in a

human in vitro

fertilization lab at

Cornell University

Medical School.

Christina Yu

Wang

Ph.D.

Physiology

Kent State

1984 Modification of Sperm

Membrane Lipids During

Epididymal Maturation and

Regulation of Lecithin

Degradation by GPC in

Principal Cells and Sperm In

Vitro

Research Scientist at

Abbott Laboratories,

North Chicago, IL.

Robert Brian

Shabanowitz

Ph.D.

Physiology

Kent State

1984 Two-Dimensional

Electrophoresis of Proteins

in Somatic Cells,

Spermatozoa, and Fluid

From Specific Regions of the

Rat Epididymis

Associate Director of

the Human In Vitro

Fertilization Lab

Geisinger Medical

Center, Danville.

Dr. Gary Killian

34

Student

Degree/Major

Year

Title

Present Position

Raymond P.

Perez

B.S.-M.D.

Honors Thesis

College of

Medicine

Kent State

1984 Early Events in Herpes

Simplex Virus Replication:

A Model for Cellular

Activation

Medical doctor,

Akron City Hospital,

Akron, OH.

Carole

Wegner

Honors Thesis

Biological

Sciences

Kent

State/Penn

State

1985 Purification of -N-

Acetylhexosaminidase From

Rat Cauda Epididymal Fluid

NIH Postdoctoral

M.D. Anderson

Cancer Institute

Tamara

McNutt

M.S.

Biological

Sciences

Kent

State/Penn

State

1985

Changes in Spermatozoal

Membrane Proteins,

Carbohydrates Activity

During In Vitro Capacitation

in the Guinea Pig.

Associate Professor

Augusta College

David

Dunlap

M.S.

Biological

Sciences

Kent

State/Penn

State

1985

Changes in Phospholipid

Composition and

Phospholipase Activity of

Guinea Pig Sperm

Membranes During In Vitro

Capacitation

IVF Laboratory

Director, St.

Elizabeth's

Medical Center,

Youngstown, OH.

Joseph Hall,

III

Ph.D.

Chemistry

Kent State

1985

Analysis of Plasma

Membrane Polypeptides and

Glycosidase Activity of

Sperm Isolated From the Rat

Epididymis

Assoc. Prof. of

Biochemistry at

North Carolina State.

Thomas

Honadel

M.S.

Animal

Science

Penn State

1987

Cryopreservation of Murine

and Bovine Embryos Using

Glycerol in Combination

With Trehalose

Veterinarian in South

Dakota.

Sharon

Henney

M.S.

Animal

Science

Penn State

1988

The Relationship Between

Bull Libido at Semen

Collection and Plasma

Concentrations of

Testosterone, Estradiol,

Prolactin Hormone

Research Director

Mainline Fertility

Center, Asst. Prof. of

OB/GYN, Temple

Univ. Med. School.

Dr. Gary Killian

35

Student

Degree/Major

Year

Title

Present Position

Robyn

Gerena

M.S.

Animal

Science

Penn State

1990

Changes in the Protein

Composition of Oviduct

Fluid During the Cow

Estrous Cycle

Staff Scientist –

Rockland

Immunochemicals

Carole

Wegner

Ph.D.

Physiology

Penn State

1990

Estrus-Associated Protein

Synthesized by the Bovine

Oviduct

NIH Postdoctoral

Fellow M.D.

Anderson Cancer

Institute. Tamara

McNutt

Ph.D.

Physiology

Penn State

1990

Influence of Oviduct and

Follicular Fluids on Bovine

Spermatozoa During In Vitro

Capacitation

Associate Professor

Augusta College

Augusta, Georgia.

Kathleen

Page

Ph.D.

Physiology

Penn State

1991

Regulation of

Oligosaccharide Biosynthesis

in the Rat Testis

Associate Professor

of Biology, Bucknell

University. Margaret

Henault

Ph.D.

Animal

Science

Penn State

1991

Lipid Droplet

Characterization and In Vitro

Lipid Synthesis of Bovine

Oviduct Epithelia

IVF Laboratory

Director, Carolinas

Medical Center,

Charlotte, NC Sharon

Henney

Ph.D.

Physiology

Penn State

1992

Interaction of the Oviduct

Epithelium with Sperm

Research Director

Mainline Fertility

Center, Assistant

Professor, OB/GYN,

Temple Univ. Med.

School. Robin King

USDA

Fellow

Ph.D.

Physiology

Penn State

1993

Isolation and

Characterization of the

Bovine Estrus-Associated

Protein

Associate Professor,

Delaware Valley

College

Carmen

Rodriguez

MS

Animal

Science

Penn State

1995

Effect of oviduct region and

stage of the estrous cycle on

oviduct fluid proteins that

associate with sperm

Assistant Professor

Commonwealth

University of VA

Dr. Gary Killian

36

Xiang Jing

Xie

MS

Physiology

Penn State

1996

Purification and

characterization of a 34 kDa

oviduct specific protein

Embryologist, IVF

Laboratory Div. of

Gynecologic

Endocrinology and

Infertility,

Washington, D.C. Aida Cancel

NIH MARC

Fellow

Ph.D.

Physiology

Penn State

1996

Isolation, characterization

and localization of the

fertility-associated protein,

BSP55, in the Holstein bull

reproductive tract.

Research Associate,

Clinical Research

Department, Family

Health International,

Chapel Hill

Carmen

Rodriguez

Ph.D.

Animal

Science

1999 Prostaglandin D synthase and

osteopontin gene expression

in the Holstein bull

reproductive tract.

Post Doctoral Fellow

Department of

Anatomy, Univ. of

Virginia Medical

School

Alexandra

Staros

Ph.D.

Physiology

1999 Role of the bovine estrus-

associated protein in

fertilization and embryonic

development in vitro.

Staff Scientist, Fox

Chase Cancer

Institute

Karen

Lavery

Ph.D.

Physiology

2001

Presence of haptoglobin-like

protein in bovine oviductal

fluid

Senior Scientist

Sequintor Scientific

Amy Way

Ph.D.

Physiology

2001

The role of oviductal fluid

catecholamines in gamete

function and early embryonic

development

Associate Professor

Lockhaven

University

Carrie

Golash

Ph.D.

Animal

Science

2002

Effect of zona pellucida

carbohydrates on sperm-egg

binding , fertilization and

embryo development.

Science Policy

Analyst

Federation of

American Societies

for Experimental

Biology

Jose Robles M.S.

Physiology

2005 Determining and Identifying

Cofactors that are Required

for Isotype Specific Class

Switch Recombination

Currently applying to

medical schools

Dr. Gary Killian

37

Thesis and Dissertations

Student

Degree/

Major

Completion

Proposed Thesis Title

David Erickson Ph.D.

Physiology

2006 Association of osteopontin with bovine

spermatozoa and its role sperm

capacitation and fertilization.

Post-doctoral Fellows under Direction of Dr. Killian

Dr. S. B. Lall, India World Health Organization Fellow, 1981-1982

Dr. Anne Grippo (Ph.D. in Medicinal Chemistry) 1987-92

Dr. Margaret Henault, 1991-93

Dr. Robin King, 1993

Dr. Omar Salama, Egypt 1993-94

Dr. Mohamed Gabr, Egypt 1993-94

Dr. Robyn Gerena, 1994-1999

Dr. Einko Topper, The Netherlands, 1996

Dr. Rene Hoier, Denmark, 1997

Dr. Dorte Johansen, Denmark, 1997-1998

Dr. Christoph Gabler, Germany, 1998-2000

Dr. Edson Guilherme Vieira, Brazil, 1998-2000

Roseli Goncalves, DVM, PhD. Brazil, 2000-2001, 2002, 2003

Ioannis Taitzoglou, DVM, Ph.D., Greece 2000, 2003

Ann-Sofi Bergqvist DVM, Sweden 2001, 2002

Ricardo Bertolla, DVM, Brazil 2001, 2003-2004

Ingrid Eder, DVM, Brazil, 2003-2004

Dr. Gary Killian

38

Carlos Eduardo Souza, DVM, M.S., Brazil 2005, 2006

Jorge Martins, DVM, Brazil 2005

Elisa Monaco, DVM, Italy 2004-2006

Arlindo Moura, Ph.D., Brazil 2003-2006

Alethia Lima Souza, DVM, M.S., Brazil 2005-2006

Killian, Invited External Presentations, 1975 – Present

1975

1. Harvard Medical School. Laboratory for Human Reproduction and Reproductive Biology,

Boston, MA. March 21 "Separation of Cell Types Comprising the Hamster Caput Epididymis

by Unit Gravity Sedimentation."

1976

2. West Virginia University, Dept. of Biology, April 12. "A New Approach to Studying the

Function of the Epididymis."

3. University of California, Riverside, Department of Biology. February 24. "Separation of

Epithelial Cells from the Epididymis."

4. Louisiana State University, Department of Zoology and Physiology. March 26. "A New

Method for Separating Cells from the Hamster and Rat Epididymis."

5. Kent State University, Kent, OH. Department of Biological Sciences. March 15. "Studies on

the Function of the Epididymis."

1985

6. Cornell University, Department of Animal Science, Ithaca, NY. March 20-21. "Studies on the

Saga of the Mammalian Spermatozoon."

1986

7. Kent State University, Kent, OH. Department of Biological Sciences. March 6, "The Male

Duct System."

8. Bucknell University, Department of Biology, Lewisburg, PA. April 25. "ESR Studies on Sperm

Capacitation."

1987

9. Eastern Mennonite College & Seminary. Suter Science Center. November 19. "Studies on

How Sperm are Regulated by Their Environment."

Dr. Gary Killian

39

10. Eastern Mennonite College & Seminary. November 20. Bioethics: A Forum Exploring the

Issues Surrounding Human Conception and Development. Two presentations: "Biomedical

Overview of Human Reproduction; Biotechnological Advances in Conception and

Development."

1988

11. University of North Carolina at Chapel Hill, Dept. of Obstetrics and Gynecology. March 15.

"Studies on the Composition of Bovine Oviduct Fluid and Its Interaction with Spermatozoa."

12. Clarion University, Biology Department, Clarion, PA. March 22. "Application of Reproductive

Biotechnology to Mammals and Man."

13. National Association of Animal Breeders, Milwaukee, WI. April 8. "Evaluation of Butylated

Hydroxytoluene (BHT) in Whole Milk Diluent for the Cryopreservation of Bull Sperm."

14. University of Pennsylvania Medical Center, Dept. Obstetrics and Gynecology. October 25 and

26. "Studies on the Composition of Bovine Oviduct Fluid and How it Influences Sperm In

Vitro" and "Studies on Signal Transduction in Sperm."

1989

15. Eastern Artificial Insemination Cooperative, Ithaca, NY. March 8. "An overview of Research

at the Dairy Breeding Research Center."

16. Cytogam, Phoenix, AZ. October 20. "Protein and lipid analyses of bovine oviduct fluid."

1990

17. University of Hawaii, Dept. of Animal Science. November 13. "Studies on the influence of

oviduct fluid on sperm and fertilization."

1991

18. Clemson University, Dept. of Animal, Dairy and Veterinary Science. December 3. "Studies on

fluid recovered by cannula from the cow oviduct."

19. University of Georgia, Depts. of Large Animal Surgery and Physiology and Pharmacology.

December 2. "Relationship between bovine oviductal proteins and the gametes."

1992

20. Denver Wildlife Research Center, Denver, CO. April 13. "Penn State's Deer Research

Facility."

21. National Association of Animal Breeders Technical Conference, Milwaukee, WI. April 24.

"Fertility Factors in Seminal Plasma."

22. National Association of Animal Breeders Technical Conference, Milwaukee, WI. April 25.

"How Does the Oviduct Influence Fertility?"

Dr. Gary Killian

40

23. American Association of Animal Science Annual Meeting, Pittsburgh, PA. August 10. "Origin

and Function of Estrus-Associated Protein in the Cow Oviduct."

24. Dept. of Obstetrics and Gynecology, Jessop Hospital for Women, University of Sheffield,

Sheffield, UK. August 21. "Role of the Oviduct in Sperm Capacitation."

25. International Congress on Animal Reproduction, The Hague, The Netherlands, August 25.

Workshop discussion leader "Zona-sperm interaction in the bovine and sperm treatment for in

vitro fertilization."

1993

26. USDA-APHIS, DWRC. Denver, CO. August 4. Research programs at the Dairy Breeding

Research Center.

27. Animal Production Research Institute, Ministry of Agriculture, Cairo, Egypt. September 29.

"Role of the Accessory Sex Glands in Affecting Sperm Fertility."

28. Department of Animal Production, Alexandria University, Alexandria, Egypt. October 2. "The

role of the Oviduct in Affecting Sperm Function."

29. Research for Animal Production (IVO-DLO) 'Schoonoord', Zeist, The Netherlands. October 8.

"Overview of Research at the Dairy Breeding Research Center."

30. Contraception in Wildlife Management Symposium. Denver, CO. October 26. "In vitro

fertilization as a bioassay for screening antisera to native and recombinant zona pellucida (ZP)

immunogen preparations developed for immunocontraception."

1994

31. Land Management and Wildlife Management Workshop, PA Game Commission, Altoona, PA.

February 23. "Deer Contraception."

1995

32. Eastern Artificial Insemination Cooperative, Ithaca, NY. March 25. “Are There Fertility

Factors in Seminal Plasma?”

33. University of Utrecht, Department of Reproduction and Herd Health, College of Veterinary

Medicine, The Netherlands, Demonstration of Surgical Techniques to Cannulate the Cow

Oviduct and Bull Vas Deferens, Utrecht, The Netherlands, April.

34. Holland Genetics, Arnham, The Netherlands. April. “Overview of Male and Female Factors

Affecting Bovine Fertility.”

35. North American Wildlife Enforcement Officers Association Annual Conference, Carlilse, PA.

July 24. “Wildlife Contraception.”

Dr. Gary Killian

41

36. International Colloquium on -Trace, Osaka, Japan. November 17. “A Fertility Associated

Protein in Bull Seminal Plasma is Prostaglandin D Synthase.”

37. University of Hawaii Medical School, Department of Anatomy and Reproductive Biology.

Honolulu, HI November 21. “Determinants of Male Fertility.”

38. Sheffield University, Jessop Womens Hospital, Dept. of OBGYN. Sheffield, UK. December

15. “Role of Seminal Plasma Affecting the Fertility of Sperm.”

39. British Fertility Society and British Andrology Society Joint Meeting. Warwick, UK.

December 19. “Role of Oviductal Fluid Modulating Sperm Physiology.”

1996

40. National Association of Animal Breeders. Milwuakee, WI. April 12. “Seminal Plasma

Proteins.”

41. Université de Montréal, Department of Medicine, Montreal, Quebec, Canada. June 12.

“Evidence for the Existence and Identity of Fertility-Associated Proteins in Seminal Plasma.”

42. Laval University, Centre of Research in Biology of Reproduction, Quebec City, Quebec,

Canada. June 13. “Seminal Plasma and Oviductal Fluid: Connections Related to Spermatozoan

Function.”

43. Royal Veterinary and Agricultural University, Department of Clinical Studies. Frederiksburg C,

Denmark. July 19. “Update of Research on Approaches to Studying the Bovine Oviduct.”

44. Technical University of Munich, Institute of Physiology, Freising, Germany. September 19.

“Proteins in Seminal Plasma and Oviductal Fluid Influencing Spermatozoan Function.”

45. International Conference on Prostalandins and Related Compounds. Vienna, Austria.

September 26. “Prostaglandin D Synthase: Connection to Male Fertility.”

1997

46. New Zealand Agricultural Research Institute, LTD. Hamilton, NZ. “Demonstration of Surgical

Techniques to Cannulate the Bull Vas Deferens”. February 26, 28, March 4, 6, 1997.

47. New Zealand Agricultural Institute Hamilton, NZ. Seminar, “Proteins in the oviduct and the

male reproductive system that influence bovine sperm fertility”: March 5, 1997.

48. Utrecht University Faculty of Veterinary Medicine, Utrecht, The Netherlands. “Male fertility

associated proteins.” April 29, 1997.

49. Holland Genetics, Arnham, The Netherlands. “Recovery of Sperm from the Bovine Oviduct

Using Indwelling Catheters.” May 2, 1997.

50. NOVA Post Graduate School for Animal Reproduction, Embryo Technology.

Dr. Gary Killian

42

Royal Veterinary and Agricultural University, Department of Clinical Studies. Frederiksburg C,

Denmark. August 29. “Fertility Associated Proteins in Bovine Seminal Plasma.”

1998

51. Osaka Bioscience Institute, Osaka, Japan, “Localization and expression of Prostaglandin D

Synthase in the Bull Reproductive Tract.” February 16, 1998.

52. National Wild. Research Center, Ft. Collins CO. Penn State results of 1997-98 for the Deer

Contraceptive Study. June 1998.

53. Wildlife Society Annual meeting, Buffalo, NY. Symposium on Status and Future of Wildlife

Fertility Control, “Effects of contraceptives on deer behavior.” September 24, 1998.

54. Royal Agricultural and Veterinary University, Dept. of Clincial Studies, Copenhagen, Denmark.

Studies on in vivo fertilization and embryo development of in vitro matured oocytes transferred

to the bovine oviduct. November 6, 1998.

1999

55. Utrecht University Faculty of Veterinarian Medicine, Utrecht, The Netherlands. Special

Symposium on Sperm Oviduct Interactions, “Influence of oviductal secretions on sperm

function.” March 1, 1999.

56. Depto. Reproducao Animal FCVA UNSD Department of Animal Reproduction, Faculty of

Veterinary Medicine, Natural University, Sao Palo State. Jaboticabol-SP, Brazil. Three day

course, “Factors affecting fertility in reproduction” concerning 1. Evidence that seminal plasma

and its components affect bovine male fertility. 2. The role of bovine oviduct secretions in

influencing sperm fertility. 3. Animal contraceptives. July 19, 20, 21, 1999.

57. XIII Brazilian Congress of Animal Reproduction, Belo Horizonte-MG, Brazil. Plenary paper,

“Male factors affecting sperm fertility.” July 29, 1999.

58. Cornell University, Reproductive Physiology Seminar Series, Ithaca, NY. “Bovine seminal

plasma protein characteristics and their relationship to fertility.” October 19, 1999.

2000

59. Tartu University, Tartu, Estonia. Two lectures, “Improving Reproductive Management of

Dairy Cattle and Dairy Cattle Genetics.” January 24, 2000.

60. Latvia Ministry of Agriculture, Riga, Latvia. Two lectures, “Improving Reproductive

Management of Dairy Cattle and Dairy Cattle Genetics.” January 28, 2000

61. Lithuania Ministry of Agriculture, Vilnius, Lithuania. Two lectures, “Improving Reproductive

Management of Dairy Cattle and Dairy Cattle Genetics.” February 1, 2000.

62. National Wildlife Research Center. Summary of Deer Contraceptive Research at Penn State

1999-2000. June 16, 2000.

Dr. Gary Killian

43

63. 14th

International Congress on Animal Reproduction Stockholm, Sweden. “New trends in

estimating fertility of semen from bulls, boars and stallions.” July 3, 2000.

64. Eastern Wildlife Damage Management Conference at Penn State University, “Behavioral

Observations and Physiological Implications for White-tailed Deer Treated with Two Different

Immunocontraceptives.” October 7, 2000.

65. University of Pennsylvania College of Veterinary Medicine, New Bolton Center. “Role of

Native Proteins in Oviductal Fluid in Modulating Gamete Function and Fertility.” October 19,

2000.

66. Institute for Hormone and Fertility Research at the University of Hamburg, Germany.

“Identification and Origin of Protein Markers for Fertility in Semen”. November 13, 2000.

2001

67. Harley Davidson, Inc, York, PA. “Strategies for Deer Management involving Contraception”

October 2, 2001.

2002

68. Texas A&M University, Keynote Speaker Reproduction Symposium. “Male Fertility Proteins in

Seminal Plasma” January 7, 2002

69. Texas A&M University, “Role of Native Proteins in Oviductal Fluid in Modulating Gamete

Function and Fertility.” January 8, 2002.

70. National Wildlife Research Center, Ft Collins, CO. June, 2002. Research Report on

Contraception of Feral Swine. For 5 yr review.

2003

71. Evaluation of GnRH contraceptive vaccine in captive feral swine in Florida. , 10th

Wildlife

Damage Management Conference, Hot Springs, AR April 5, 2003

72. Demonstration of surgical techniques to cannulate the oviducts of Italian buffalo. DISCIZIA

Facoltà di Medicina Veterinaria, Università Federico II, Naples, Italy December 1-4.

2004

73. Evaluation of three contraceptive approaches for population control of wild horses. Vertebrate

Pest Conference, Vasilia, CA. March 1, 2004

74. DISCIZIA Facoltà di Medicina Veterinaria, Università Federico II, Naples, Italy. Invited lecturer

to present research on male and female reproductive tract secretions, surgical models, contraception

of wildlife, and US dairy farm profitability. May 14-21.

Dr. Gary Killian

44

75. Central Science Laboratory, York, United Kingdom "Contraception of Wildlife and Feral

Species, Potential Tools

for Population Control" May 23.

76. University of San Paulo, School of Veterinary Medicine and Animal Science. San Paulo, Brazil.

Invited lecturer to present research on male and female reproductive tract secretions, surgical

models, contraception of wildlife, and US dairy farm profitability. June 7-14.

77. Evidence for the role of oviduct secretions in sperm function, fertilization and embryo

development. Plenary Paper, 15th

International Congress on Animal Reproduction. Porto Seguro,

BA-Brazil. August 8-12.

78. Lecturer in course on Diagnostic Andrology, The Nordic Course, Swedish University of

Agricultural Science, Uppsala. September 6-11.

2005

79. Observations on the Use of the GnRH Vaccine GonaConTM

in Male White-tailed Deer

(Odocoileus virginianus). Wildlefe Damage Management Conference, Traverse City, MI. May 16-

18.

80. Annual Report on Deer Contraception. National Wildlife Research Center, Ft. Collins, CO. July

5-6.

81. Identification of proteins from the accessory sex gland fluid that influence the oocyte penetrating

capacity of bovine cauda epididymal sperm in vitro. Annual Meeting of the Society for the Study of

Reproduction, Quebec City, August 3-4.

2006

82. Twelve Years of Contraception Research on White-tailed Deer: What have we learned?

2nd

International Workshop on Wildlife Fertility Control. February 28-March 2. Ft. Collins, CO.

83. Safety and Toxicity Evaluation of GonaConTM

Immunocontraceptive Contraceptive Vaccine in,

White-tailed Deer (Odocoileus virginianus). 22nd

Vertebrate Pest Conference, March 6-9 Berkley,

CA

84. Long-term Efficacy of Three Contraceptive Approaches for Population Control of Wild Horses.

22nd

Vertebrate Pest Conference, March 6-9 Berkley, CA

85. Annual Report and Project Program Review of Contraception Research at Penn State. USDA-

APHIS, National Wildlife Research Center, Ft Collins, CO. August 8-9.

Dr. Gary Killian

45

86. Justification for evaluating the GnRH vaccine GonaConTM

for population control of male and

female dogs. Alliance for Contraception of Cats and Dogs, Annual meeting, Washington D.C.

November 9-10.

87. Changes sperm undergo in the female reproductive tract. Rupert Amann 75th

Birthday

Symposium, Ft Collins December 15

Summary Totals of Invited Presentations, 1995 - 2006

Year Domestic International Total

1995 3 5 8

1996 1 5 6

1997 0 5 5

1998 2 2 4

1999 3 3 6

2000 3 5 8

2001 1 0 1

2002 3 0 3

2003 1 1 2

2004 1 4 5

2005 2 1 3

2006 5 1 5

Total all years 25 31 56

Reviews of Journal Articles and Grants Conducted by Dr. Killian, 1993-Present

Journal Papers (several each year for each journal)

Biology of Reproduction

Journal of Reproduction and Fertility

Journal of Andrology

Domestic Animal Reproduction

Thereogenology

Animal Reproduction Science

Grants Reviewed

The Welcome Trust

Medical Research Council of Canada (1-2 per year)

Lalor Foundation (1-2 per year)

NRI-USDA - Reproductive Efficiency (Ad hoc reviews 2-3 grants per year)

NRIUSDA Reproductive Efficiency Grant Award Panel 1992

NIH-NICHD - Reproductive Biology Study Section member 1994, 1996, 1998, 2003

Dr. Gary Killian

46

(approximately 15 grants each year for NIH)

gary killian, curriculum vitae

Documents