does down-regulation of photosynthetic capacity by elevated co2 depend on n supply in dactylis...

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Physiologia PlantarumAn International Journal for Plant Biology

Author guidelinesThe instructions below have been formulated to help authors prepare their manuscript, and if they are fol-lowed carefully the time between receipt, review and publication will be minimised. Failure to follow the instructions may make it necessary for the manuscript be returned for correction, with a consequent delay in review and publication. Failure to follow normal scientific standards or a lack of readability may lead to rejection of the manuscript.

Manuscripts are accepted only in English and spel-ling should conform to that in the Concise Oxford English Dictionary or Merriam-Webster’s Collegiate Dictionary.

Fees and privilegesSix printed pages (one printed page of full text is equi-valent to 5600 characters, including spaces) per pa-per are offered free of charge. Pages in excess of six must be paid for by the author (GBP45/USD85 per page). It is therefore in the interest of the authors to focus their papers as much as is practical. Verbose manuscripts will be returned with a request for con-densation.

Physiologia Plantarum offers authors free colour-on-web service, where they may have colour figures published on the journal website free of charge. In addition, the Journal offers authors three colour figu-res free of charge in the printed version, providing the colour is deemed necessary by the editor. Subsequent printed colour figures will be chargeable (4th figure GBP150, subsequent figures GBP50 each). A Colour Work Agreement form must be submitted to the Publisher before the manuscript can be published.

OnlineOpenOnlineOpen is available to authors of primary re-search articles who wish to make their article freely available to all on publication, or whose funding agency requires grantees to archive the final version of their article. With OnlineOpen, the author, the author’s funding agency, or the author’s institution pays a fee to ensure that the article is made freely available to all upon publication via Wiley Online Library, as well as deposited in the funding agency’s preferred archive. Authors choosing OnlineOpen will retain copyright in their articles and will be offered a choice of creative commons licenses. For the full list of terms and conditions, see http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1399-3054/home-page/FundedAccess.html.

Any authors wishing to send their paper Online-Open will be required to complete the payment form available from our website at: https://authorservices.wiley.com/bauthor/onlineopen_order.asp.

Prior to acceptance there is no requirement to in-

form the Editorial Office that you intend to publish your paper OnlineOpen if you do not wish to. All OnlineOpen articles are treated in the same way as any other article. They go through the journal’s stan-dard peer-review process and will be accepted or re-jected based on their own merit.

CopyrightAuthors submitting a manuscript do so on the under-standing that the work has not been published befo-re, is not being considered for publication elsewhere and has been read and approved by all authors. Your article cannot be published until the publisher has received the appropriate signed license agreement. Once your article has been received by Wiley for production the corresponding author will receive an email from Wiley’s Author Services system which will ask them to log in and will present them with the appropriate license for completion. After submission authors will retain the right to publish their manus-cript in various media/circumstances and authors should take particular note that the Society licenses to them:a. After publication of the final published version,

the right to self-archive the original submitted manuscript on their personal website or in their institution’s/employer’s institutional repository or archive. This right extends to both intranets and the Internet. The Author may not update the sub-mitted version or replace it with the published paper. The version posted must contain a legend as follows: This is the pre-peer-reviewed version of the following article: FULL CITE, which has been published in final form at [Link to final article].

b. The right to transmit, print and share copies with colleagues.

For their full rights concerning re-use of the accepted or final published versions, authors should consult the copyright form. Permission to reuse copyrighted materials can be obtained via the article’s abstract page on the journal’s website.

Plagiarism detectionManuscripts submitted to Physiologia Plantarum will be subjected to plagiarism detection and will be checked against the iThenticate database to safeguard the originality of the published text. Manu scripts that are found to include copied text will either be rejec-ted outright or, if minor, returned to authors for cor-rection.

Cover letterAuthors should include a cover letter detailing the key findings of their manuscript. The cover letter should highlight the novel aspects of their data and

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briefly describe how the authors feel their results will generate progress in their field. If the species investi-gated is not a common experimental species, the aut-hors should indicate what basic scientific/biological reason dictated their choice of species and what new insights have been gained from its use. Furthermore, if the authors feel their work merits publication as a breakthrough paper, they should indicate this in the cover letter and highlight the reasons that they feel justify expedited handling of their manuscript.

Online submissionPhysiologia Plantarum only accepts manuscripts via our online submission site. For online submissions, please visit our ScholarOne Manuscripts site at http://mc.manuscriptcentral.com/ppl. Prepare your manu-script using a word processing program and save it as a DOCX, DOC, or RTF file. Image files such as TIFF, JPEG, PDF or EPS may be uploaded. All uploaded fi-les will be combined to a single PDF and to a tagged HTML proof for peer review. The original files that you upload will be saved and can be accessed by the editorial office if necessary. Note: PDF should not be used for the original text file. Please submit names and email addresses of 4 potential reviewers. Infor-mation may also be given to avoid potential conflicts of interests.

Formats, style and organization

General

In order for manuscripts to be suitable for publishing as Online Accepted papers, authors are required to adhere strictly to the following formatting guidelines. The entire manuscript must be typed 1.5-spaced. Text should be typed in Times New Roman, 11 point. Left and right margins should be 25 mm. All pages must be paginated. Italics should be used only for scienti-fic names, genes and other terms where appropriate. Footnotes should be avoided.

Units and numerals

The SI system should be used throughout. Use nega-tive indices rather than solidus (e.g. mg l–1, not mg/l). Use space between numeral and unit (e.g. 14 h, not 14h) and between units (e.g. µmol m–2 s–1). Thousands should be separated by spaces (e.g. 1000, 10 000, 100 000).

Layout

The layout of the manuscript should be as follows:• Title• Fullname(s)ofauthor(s)(thecorrespondingaut-

hor should be indicated)• Address(es) of author(s) (including e-mail ad-

dress for the corresponding author)• Abstract

• Abbreviations• Introduction• Materialsandmethods• Results• Discussion• Authorcontributions• Acknowledgements• References• SupportingInformation• Tables (included in themain textdocumentor

submitted as separate documents)• Figurelegends(includedinthemaintextdocu-

ment or submitted as a separate document)• Figures(submittedasseparatedocuments)

AbstractThe abstract must not exceed 250 words in length and should clearly state the problem being addres-sed, the methods employed and the main results. The scientific name(s) and, where applicable, cultivar or variety of the plant(s) used should be spelled out in full in the abstract. EC numbers should be given for enzymes mentioned. The Abstract should not be divi-ded into paragraphs.

AbbreviationsNecessary explanations of abbreviations used should be listed alphabetically and have the form: DPC, diph enylcarbazide; PQ, plastoquinone; ROS, reac-tive oxygen species.

Standard abbreviations need not be defined in this section. Avoid complicated abbreviations of your own that make reading difficult. Abbreviations in the Introduction and following text should generally be written out in full with each abbreviated form in pa-renthesis the first time that it is mentioned.

IntroductionThis should be concise, giving the reader an out-line of the subject, presenting the “state-of-the-art”, leading into the reasons for the present study and its aims. Text references should have the form: Gould and Lister (2005) or Dixon et al. (2005), if more than two authors. Multiple text references should be cited alphabetically (Bowler et al. 1989a, 1989b, 1991, Chang and Kaufman 2000, Legendre et al. 1993, Tsang et al. 1991). Comma is the separator between references, but there should be no comma between author name and year. Unpublished data and perso-nal communications should have the form: (Smith et al., unpublished data), (Hosino et al. personal com-munication).

Materials and methodsGive the full scientific name(s) of plant(s) used, as well as the cultivar (cv.) or variety (var.), where app-licable. Spell out genus name at first mention abbre-

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viated thereafter: e.g. Eucalyptus globulus in article title, at first mention in Abstract, main text, Tables, Figures or at the beginning of a sentence, but E. glo-bulus thereafter.

All growth conditions should be properly descri-bed. Include the trade name and manufacturer of all lamps used, as well as irradiance (W m–2) or photo-synthetic photon flux density (µmol m–2 s–1); lux or µE are not acceptable. The trade name(s) and suppliers of apparatus and chemicals used should also be gi-ven. EC numbers should be given for enzymes used.

If the data includes microarray analysis, authors should refer to the MIAME recommendations: http://www.mged.org for guidance in preparing their ma-nuscripts.

ResultsMake the text (written in the past tense) as objective and descriptive as possible. Refer to figures and tables as Fig./Figs. and Table/Tables. It is important that re-ferences to figures and tables are consistent through-out the manuscript so that figure and table tags work properly in the HTML proof.

DiscussionThe Discussion should not merely repeat the cata-logue of results. The authors should show how their findings advance our knowledge and understanding of the topic they are investigating. The Discussion should be concisely written with the main points of the work logically presented and with reference to the relevant tables, figures and literature.

Author contributionsThe contributions made by all listed authors should briefly be described. Physiologia Plantarum asks all authors to follow the guidelines outlined in the Com-mittee on Publication Ethics (COPE) report from 2003, derived from the International Committee of Medical Journal Editors (ICMJE). For additional information the COPE report and the International standards for authors report from 2010 detail general guidelines for the preparation of manuscripts and recommenda-tions for ethical authorship. These documents can be downloaded from http://publicationethics.org/.

ReferencesFor formatting instructions regarding in-text reference citations, please see the Introduction section in this document. The reference list should be formatted ac-cording to the Harvard style (name and date):

Journal

Arap RD, Hagbedorn M Jr, Koivunen E, Pasqualini R, Ruoslahti E (1998a) Molecular heterogeneity of the vascular endothelium revealed by in vivo phage display. J Clin Invest 102: 430–437

Harakeh S, Jariwalla RJ, Pauling L (1998b) Suppres-sion of human immunodeficiency virus replication by ascorbate in chronically and acutely infected cells. Proc Natl Acad Sci USA 87: 1745–1749

Hossain M, Asada K (1987) Ascorbate-regenerating enzymes in chloroplasts. Ind J Biochem Biophys 24(suppl 6): 52–57

Book

Freshney RI (1994) Culture of Animal Cells, 3rd Edn, Vol. 11. Wiley-Lisds, New York, pp 86–95

Marsh JF (2001) Analysis of arbuscular mycorrhizas, 2nd Edn. Kluwer Academic, Dordrecht

Edited book

Galston AW, Kaur-Sawhney R (1995) Polyamines as endogenous growth regulators. In: Davies PJ (ed) Plant Hormones: Physiology, Biochemistry and Molecular Biology, 2nd Edn. Kluwer Academic, Dordrecht, pp 158–178

Marsh JF (ed) (2001) Analysis of arbuscular mycorrhi-zas, 2nd Edn. Kluwer Academic, Dordrecht

Herrmann S, Munch J-C, Buscot F (2001) Assays to study angiogenesis. In: Voest EE, D’Amore PA (eds) Tumor Angiogenesis and Microcirculation, 2nd Edn. Marcel Dekker Inc., New York, pp 91–102

Report

Kramer PJ, Boyer JS (1995) Yield and quality of green-house grown tomatoes irrigated with saline water. Annual Report of ARO, The Volcani Center 307-0227: 1–18 (in Hebrew)

Online report

Estonian Human Development Report 2002. The quality of greenhouse grown tomatoes. Available at http://www.iiss.ee/nhdr/2002 (accessed 17 Sep-tember 2008)

Biogenex (2006) Data sheet for liquid DAB substrate pack. Available at http://www.biogenex.com/doc/datasheets/English/932-HK130.pdf (accessed 17 September 2008)

Thesis

Gullberg J (2005) Metabolomics: A tool for studying plant biology. DPhil Thesis. Swedish University of Agricultural Sciences, Umeå. Acta Universitatis Agriculturae Sueciae 2005:88, ISBN 91-576-6987-2

In press

Fernandez V, Winkelmann G The determination of ferric iron in plants using the microbial iron che-lator desferrioxamine E and HPLC. Biometals, in press

Conference

Anderson B, Katus K, Puur A, Silver B (1993) Cha-

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racteristics of women having abortions in Estonia. International Population Conference, Vol. 1. IUSSP, Montreal

Herrera-Carillo Z, Torres MS, Singh AP, Vorsa N, Gi-anfagna T, Meyer W, White JF Jr (2009) Phenolic, flavonoid and antioxidant profiling for cool-season grasses with and without endophyte. Proceedings of the 18th Annual Rutgers Turfgrass Symposium, 12 Jan 2009, Rutgers University, New Brunswick, NJ, USA, pp 43

Monograph

Arap RD, Hagbedorn M Jr, Koivunen E, Pasqualini R, Ruoslahti E (1998) Category of young children un-derstanding of pretense. Monographs of the Socie-ty for Research in Child Development, 58(1, Serial No. 231)

Brundrett M, Bougher N, Dell B, Grave T, Malajczuk N (1996) Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research Monograph 32, Canberra, pp 374

References should be listed alphabetically according to the first named author. Where there are multiple references by the same first author, the order in the list should be:• Singleauthor.Wheremorethanonereferenceis

given for a single author the publications should be listed chronologically.

• Twoauthors(shouldcomebefore3ormoreaut-hors). These should be arranged first alphabeti-cally, then chronologically.

• Threeormoreauthors.Thesereferencesshouldbe arranged chronologically.

List all authors (no et al.). Journal titles are abbre-viated without full points (e.g. J Clin Dermatol). Do not abbreviate one-word journal titles (e.g. Phyto-chemistry). Do not give issue numbers for journal ar-ticles. No full points at end of references. Publisher name and place of publication [city, state (if USA)] should be given for book references.

Unpublished material (except for accepted manu-scripts in press) and personal communications should only be cited in the text, and not in the references. In the text, the initials and last names of all colla-borators should be given where unpublished work is cited. Theses may not be cited in References unless they have been published (include ISBN, or number in Dissertation Abstracts). Unpublished theses may be cited only in the text as follows: (R. Y. Smith 2007. Thesis, Univ. of California, Davis, CA, USA).

Supporting InformationAuthors are welcome to submit additional ”Sup-porting Information”, such as data sets or additional figures or tables. This material will not be published

in the print edition of the journal but it will be view-able via the online edition. Such supporting informa-tion should be referred to in the text as, for example, ”see Appendix S1 in Supporting Information”.

Supporting files are hosted by the Publisher in the format supplied by the author and are not copy-edited by the Publisher. It is the responsibility of the author to supply supporting information in an app-ropriate file format and to ensure that it is accurate and correct. Prior to publication, authors will be sent the URL of their Supporting Information for them to check the content. Extensive editing of material is not possible at this stage and the author has the responsi-bility to ensure that material is sent in a correct form at the time of submission.

Authors should include a ’Supporting Information’ section immediately after their References section, which should be in the following form:

Supporting InformationAdditional Supporting Information may be found in the online version of this article:Appendix S1. Short title of supplementary ap-pendix S1Appendix S2. Short title of supplementary ap-pendix S2

Only short titles to appendices should be given in this section; full titles can be given with the Supporting Information itself.

TablesEach table should be on a separate page furnished with explanatory headings. Tables must be planned to fit a printed width of either 80 or 166 mm. Footnotes are usually not allowed. Tables should be submitted as editable text files, such as DOCX, DOC, or RTF.

FiguresEach complete figure should be placed in a sepa-rate file. Save photographic images in TIFF or JPEG format at a resolution of no less than 300 dpi. Line art and combination figures should be saved in PDF, EPS, TIFF, or JPEG format at a resolution of no less than 600 dpi. The colour mode for black and white figures should be greyscale. Colour images should be saved in RGB colour mode. Save TIFF files using LZW compression. JPEG files must be saved with a com-pression setting for high graphical quality rather than small file size.

Figures should be planned to appear with a maxi-mum final width of 80 mm (single-column), 125 mm (1.5 column) or 166 mm (double-column). The font used in figures should be either Helvetica regular or Arial regular. Letters, numbers and symbols must ap-pear clearly but not oversized. A suitable final size for lettering is 1–2 mm at reproduction size. One uni-

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form size throughout is generally recommended.Avoid complicated symbols or patterns. Use open

and closed circles, squares and triangles; open, stri-ped and closed bars in histograms. Graphs and his-tograms should be boxed in and scale marks (turning inwards) provided. Lines should be clear, but not thick and heavy.

Full artwork guidelines are available from the Publisher’s website: http://authorservices.wiley.com/bauthor/illustration.asp.

Colour vision impaired readers

When preparing your figures please take into account that some readers have deficient colour vision. Ima-ges of fluorescent double-staining micrographs and DNA chips should preferably not contain a combina-tion of red and green. Please use magenta and green

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instead. Also avoid using red characters on a dark background. In colour graphs and line art, use both colour and shape (different symbols and line types) to convey information. More detailed information can be found at: http://jfly.iam.u-tokyo.ac.jp/color/.

Image processing

Photographic images submitted to the journal should be minimally processed. No parts, regions or specific features of an image may be changed, moved, remo-ved, obscured or enhanced. Changes to brightness, contrast and colour balance are allowed if they are applied to the whole image and equally to controls and provided no information in the original is misre-presented by the adjustments.

Images from different parts of a gel, or from diffe-rent gels, that are grouped into a single figure must be clearly separated, e.g. with dividing lines.

All images in manuscripts accepted for publication will be scrutinized by the editorial office. Any indica-tion of improper image manipulation will be repor-ted to the Editor-in-Chief, who may request original image files and/or data from the authors. Failure to comply may lead to the acceptance of the manuscript being revoked.

Colour figures

Physiologia Plantarum offers authors free colour-on-web service, where they may have colour figures published on the journal website free of charge. In addition, the Journal offers authors three colour figu-res free of charge in the printed version, providing the colour is deemed necessary by the editor. Subse-quent printed colour figures will be chargeable (4th

figure GBP150, subsequent figures GBP50 each). A Colour Work Agreement form must be submitted to the Publisher before the manuscript can be publis-hed (http://www.blackwellpublishing.com/pdf/SN_Sub2000_F_CoW.pdf).

Cover photographsAuthors are welcome to submit high quality photo-graphs suitable for the cover of Physiologia Planta-rum. They should be supplied as digital images with a resolution of no less than 300 dpi at a reproduction size of 213 mm wide × 174 mm tall. Digital images should be saved in TIFF format using LZW compres-sion. Potential cover photographs should be sent to the Editorial Office and be accompanied by a brief descriptive summary.

Production contactThe Colour Work Agreement Form should be retur-ned to the Production Editor:

Customer Services (OPI)John Wiley & Sons Ltd,

European Distribution CentreNew Era EstateOldlands WayBognor RegisWest SussexPO22 9NQUK

The Colour Work Agreement Form can also be acqui-red by contacting the Production Editor who will be able to email or fax a form to you.

PublicationPublication in Physiologia Plantarum implies an obli-gation on the part of authors to deposit any novel nucleic acid sequence data referred to in their pa-pers with the EMBL Data Library. Deposition of such data should, at the latest, be made immediately after the paper has been accepted for publication, so that the authors can add the relevant accession number from the Data Library at the end of the text at the page proof stage. Details of how to submit data to the EMBL Data Library can be found in Nucleic Acids Research, Vol. 18, issue 1.

After editorial review and acceptance, the manus-cript is sent to the Publisher. The attention of the Edi-torial Office should be drawn to significant errors that are discovered after publication, so that they may be included in a list of Errata.

Proofs

The corresponding author will receive an email alert to download a PDF (portable document format) file of the proof. Adobe Reader or similar PDF viewer will be required in order to read this file. This will enable the file to be opened, read on screen, and printed out in order for any corrections to be added. Further instructions will be sent with the proof. Changes against the manuscript at this stage will be billed at cost if they exceed 5% of the total.

Offprints

Corresponding authors will receive PDF offprints of their paper, with web-optimised figures, via email free of charge. Printed copies with high resolution fi-gures may be ordered at prices quoted on the order form which accompanies proofs, provided that the form is returned with the proofs. The cost is more if the order form arrives too late for the main print run. Printed offprints are normally dispatched within three weeks of publication of the issue in which the pa-per appears. These will be charged in GBP by Wiley-Blackwell Publishing.

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Online AcceptedArticles in this journal which have been peer-reviewed and accepted, but not yet copy-edited, are published online through our Online Accepted feature in advan-ce of print publication. Go to http://onlinelibrary.wi-ley.com/journal/10.1111/(ISSN)1399-3054/accepted to see the articles currently available. At this time Sup-porting Information cannot be published at the Online Accepted stage. Any required Supporting Information will be available online from the Early View stage.

Early ViewPhysiologia Plantarum is covered by Wiley-Blackwell Publishing’s Early View service. Early View articles are complete full-text articles published online in advance of their publication in a printed issue. Artic-

les are therefore available as soon as they are ready, rather than having to wait for the next scheduled print issue. Early View articles are complete and final. They have been fully reviewed, revised and edited for publication, and the authors’ final corrections have been incorporated. Because the articles are in final form, no changes can be made after online publica-tion. The nature of Early View articles means that they do not yet have volume, issue or page numbers, so Early View articles cannot be cited in the traditional way. They are therefore given a Digital Object Iden-tifier (DOI), which allows the article to be cited and tracked before it is allocated to an issue. After print publication, the DOI remains valid and can continue to be used to cite and access the article.

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Guidelines for preparing Minireview and Special Issue manuscriptsPhysiologia Plantarum has a long history of publish-ing short, timely, topical reviews with the aim of hel-ping both researchers and students keep up-to-date with the most recent developments in the plant sci-ences. We also publish Special Issues of the Journal that contain collection of papers providing an in-depth review of recent developments in selected re-search fields.

In keeping with the objective of providing up-to-date information to researchers and students in fast moving research fields, Minireview and Special Issue papers should focus only on the recent advances in the field, not the history of the subject, emphasizing aspects that are most important for the future deve-lopment of the field or highlighting the implications of some recently published primary research data.

Minireview and Special Issue papers, which are sub-ject to peer-review, must be written concisely, with no more than 50 references and a maximum of 6 printed pages, including all references and data displays (one printed page of full text is equivalent to around 5600 characters, including spaces). The manuscript should include a maximum of 4 data displays, with an emp-hasis on integrative models rather than primary data. Authors are encouraged to submit these data displays in colour and, if found suitable, colour displays will be published free of charge.

Minireview manuscripts will be considered in all areas of experimental plant science and it is recom-mended that the Editor-in-Chief or the relevant Sub-ject Editor be contacted in advance to confirm the suitability of the chosen topic.

does down-regulation of photosynthetic capacity by elevated co2 depend on n supply in dactylis...

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