design of experiments for germination of vigna radiata
DESCRIPTION
Design of experiments for germination of vigna radiata. 徐任飞,金雯,马莹,包璐璐,余贞富. Agenda. Introduction Design of experiment Data analysis Data analysis after data transformation Conclusion Q&A. Introduction. Background and Objective. Temperature Humidity Air Moisture Light condition - PowerPoint PPT PresentationTRANSCRIPT
Design of experiments for germination of vigna radiata徐任飞,金雯,马莹,包璐璐,余贞富
Agenda
• Introduction• Design of experiment• Data analysis• Data analysis after data transformation• Conclusion• Q&A
INTRODUCTION
Background and Objective
• Temperature• Humidity• Air• Moisture• Light condition• Germinating bed• Soak time• Solution strength for soak
Literature ReviewTitle Author Conclusion
三种豆科植物种子荫发特性的研究
廖建良,贺握权,许灿英,尹艳
Temperature: 15 to 20 centigradeIllumination: darkGerminating bed: on or between paper
绿豆的浸泡工艺及其对绿豆种子萌发的影响研究
吴小勇,曾庆孝,田金河,柳明鲜 Soak time: 6 to 10 days
盐胁迫对绿豆种子萌发的影响
张秀玲Solution strength for soak: neutral salt has no effect on germination, while has effect on growing of sprout. Basic salt has effect on both the two.
DESIGN OF EXPERIMENT
Design methodology
• Response variable– Germination percentage
– Where n is the number of germinated seeds– N is the total number
– Germination rate
– Where Dt is the number of days from growing– Gt is the number of seeds germinated at that day
Design methodology
• Input variable
Variable Control level
Low ( -1) High ( 1)Light condition No sunlight
Night and day
alternate
Seed soaking time 1 day 5 day
Culture medium Above napkin Soil surface
Seed soaking solution
400mmol/L NaCl Water
Design methodology
• Noise – Quality of the air
• The same experiment location and time – Quality of the seeds
• The same lot– Temperature
• The same experiment location and time
Design of the experiment
• The 24-1 fractional factorial design– Alias structure– Resolution IV design
Defining relation: I=ABCD[A]->A+BCD
[B]->B+ACD
[C]->D+ABD
[D]->C+ABC
[AB]->AB+CD
[AC]->AC+BD
[AD]->AD+BC
Design of the experiment
• Design matrix
Run
Basic designSeed
soaking solution
( D=ABC)
Light
condition
( A)
Seed
soaking time
( B)
Culture
medium
( C)1 -1 -1 -1 -1
2 1 -1 -1 1
3 -1 1 -1 1
4 1 1 -1 -1
5 -1 -1 1 1
6 1 -1 1 -1
7 -1 1 1 -1
8 1 1 1 1
Experiment implementation
– 8 groups with 30 Vigna radiatas each– Soak the vigna radiatas which need 5 days
soaking on the May, 22 at 23:20– Soak the vigna radiatas which need 1 days
soaking on the May, 26 at 23:20– Plant all of them on the May, 27 at 23:20– Water 2 or 3 times per day to keep culture
medium humid– Check the germination condition at 23:20
each day and record the data– Calculate the germination rate and
percentage
DATA ANALYSIS
Main effects
1-1
100
90
80
701-1
1-1
100
90
80
701-1
光照条件
平均值
浸种时间
培养基类别 浸种溶液
发芽率 主效应图数据平均值
Main effects
1-1
25
20
15
101-1
1-1
25
20
15
101-1
光照条件
平均值
浸种时间
培养基类别 浸种溶液
发芽速率 主效应图数据平均值
Interactions
1-1 1-1100
80
60100
80
60100
80
60
1-1
100
80
601-1
光照条件
浸种时间
培养基类别
浸种溶液
-11
条件光照
-11
时间浸种
-11
类别培养基
-11
溶液浸种
发芽率 交互作用图数据平均值
1-1 1-1
24
16
824
16
824
16
8
1-1
24
16
81-1
光照条件
浸种时间
培养基类别
浸种溶液
-11
条件光照
-11
时间浸种
-11
类别培养基
-11
溶液浸种
发芽速率 交互作用图数据平均值
Factor effects estimation
3020100- 10- 20- 30
99
9590
80706050403020
105
1
效应
百分比
A 光照条件B 浸种时间C 培养基类别D 浸种溶液
因子 名称
不显著显著
效应类型
效应的正态图 Al pha = . 05)(响应为 发芽率,
Lenth PSE = 11. 2463的
302520151050
98
95
908580
706050403020100
绝对效应
百分比
A 光照条件B 浸种时间C 培养基类别D 浸种溶液
因子 名称
不显著显著
效应类型
效应的半正态图 Al pha = . 05)(响应为 发芽率,
Lenth PSE = 11. 2463的AB
B
C
AC
D
AD
A
403020100
项
效应
42. 33A 光照条件B 浸种时间C 培养基类别D 浸种溶液
因子 名称
Pareto 效应的 图 Al pha = . 05)(响应为 发芽率,
Lenth PSE = 11. 2463的
Statistical testing
Model refinement
3210- 1- 2- 3- 4
99
9590
80706050403020
105
1
标准化效应
百分比
不显著显著
效应类型
光照条件
标准化效应的正态图 Al pha = . 05)(响应为 发芽率,
543210
1. 0E+02
99. 98
9895
8580
50
200
绝对标准化效应
百分比
不显著显著
效应类型
光照条件
标准化效应的半正态图 Al pha = . 05)(响应为 发芽率,
光照条件
43210
项
标准化效应
2. 447
Pareto 标准化效应的 图 Al pha = . 05)(响应为 发芽率,
Statistical testing
发芽率 =83.75-14.58*光照条件
Residual analysis
20100-10-20
99
90
50
10
1
残差
百分比
100908070
20
10
0
-10
拟合值
残差
20151050-5-10
4. 8
3. 6
2. 4
1. 2
0. 0
残差
频率
87654321
20
10
0
-10
观测值顺序
残差
正态概率图 与拟合值
直方图 与顺序
发芽率 残差图
Factor effects estimation
50- 5- 10- 15
99
9590
80706050403020
105
1
效应
百分比
A 光照条件B 浸种时间C 培养基类别D 浸种溶液
因子 名称
不显著显著
效应类型
A
效应的正态图 Al pha = . 05)(响应为 发芽速率,
Lenth PSE = 1. 72125的
14121086420
98
95
908580
706050403020100
绝对效应
百分比
A 光照条件B 浸种时间C 培养基类别D 浸种溶液
因子 名称
不显著显著
效应类型
A
效应的半正态图 Al pha = . 05)(响应为 发芽速率,
Lenth PSE = 1. 72125的AC
AB
C
D
AD
B
A
14121086420
项
效应
6. 48A 光照条件B 浸种时间C 培养基类别D 浸种溶液
因子 名称
Pareto 效应的 图 Al pha = . 05)(响应为 发芽速率,
Lenth PSE = 1. 72125的
Statistical testing
Model refinement
420- 2- 4- 6- 8- 10- 12
99
9590
80706050403020
105
1
标准化效应
百分比
不显著显著
效应类型
浸种时间
光照条件
标准化效应的正态图 Al pha = . 05)(响应为 发芽速率,
121086420
1. 0E+02
99. 98
9895
8580
50
200
绝对标准化效应
百分比
不显著显著
效应类型
浸种时间
光照条件
标准化效应的半正态图 Al pha = . 05)(响应为 发芽速率,
浸种时间
光照条件
121086420
项
标准化效应
2. 57
Pareto 标准化效应的 图 Al pha = . 05)(响应为 发芽速率,
Statistical testing
发芽速率 =17.081-6.416*光照条件 -2.189*浸种时间
Residual analysis
3. 01. 50. 0-1. 5-3. 0
99
90
50
10
1
残差
百分比
25201510
1
0
-1
-2
拟合值
残差
10-1-2
3
2
1
0
残差
频率
87654321
1
0
-1
-2
观测值顺序
残差
正态概率图 与拟合值
直方图 与顺序
发芽速率 残差图
The response surface(GP)
• Soaking time has no significant effect and darkness can maximize GP
• No interaction
The response surface(GR)
• One day soaking and darkness can maximize GR
• No interaction
Response optimization
DATA ANALYSIS AFTER DATA TRANSFORMATION
Data Transformation
• Data transformation– Bounded response variable ->
unbounded
Main effects
Main effects
Interactions
Factor effects estimation
Model refinement
Statistical testing
发芽率 (变换 )=3.608-2.681*光照条件 -1.614*浸种时间 +1.307*光照条件 *浸种时间
Residual analysis
Factor effects estimation
Model refinement
Statistical testing
发芽速率 =17.081-6.416*光照条件 -2.189*浸种时间
Residual analysis
The response surface(transformed GP)
• One day soaking and darkness can maximize GP• Interaction effect between soaking time and light
condition.
Response optimization
Conclusion
• Light condition and seed soaking time affect germination percentage and germination rate significantly, while culture medium and seed soaking solution do not have great influence on GP and GR.
• Darkness and one day soaking time promote germination separately. Interaction effect between soaking time and light condition affects germination percentage.
• The best solution is no sunlight and one day soaking time for both maximal GP and GR.
Q&A