december 2008 month in review

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Herron Todd White Month In Review December 2008


  • 1. Biosafety in Microbiological and Biomedical Laboratories3rd EditionJY Richmond & RW McKinney (eds.) 5th National Symposium on Biosafety:A Rational Basis for Biocontainment

2. BMBL Introduction1941 - Meyer and Eddie 74 lab associated brucellosis infections in US 1949 - Sulkin and Pike 222 viral infections (21 fatal) Only 27% related to known accidents 3. BMBLIntroduction1951,1965, 1976 - Sulkin and Pike Surveys for lab-associated infections More than 5,000 labs Cumulative total of 3,921cases cited Most commonly reported:i Hepatitis i Brucellosisi Tuberculosis i Tularemiai Typhoidi Venzuelan Equine Encephalitis 4. BMBLIntroduction1951,1965, 1976 - Sulkin and Pike (cont.) Surveys for lab-associated infections Fewer than 20% associated with knownaccidents Exposure to infectious aerosolsplausible (but unconfirmed) for >80% ofreported cases 5. Principles of BiosafetyIntroductionBiosafety Levels 1-3 Guidelines to describe combinations of: Laboratory Practices and Techniquesi Standard Practicesi Special Practices Safety Equipment (Primary Barriers) Laboratory Facilities (Secondary Barriers) 6. Principles of Biosafety IntroductionBiosafety Levels 1-3 Provide Increasing levels of personnel and environmental protection Guidelines for working safely in microbiological and biomedical laboratories 7. Lab Practices and Techniques Introduction Knowledgeable supervisor Personnel i Aware of potential hazards i Proficient in practices/techniques Biosafety manual specific to lab 8. Safety Equipment (Primary Barriers) - Introduction Biosafety cabinets (BSCs) [BSL-2/3] Personal protective clothingi Glovesi Gowns Pipetting Devices Safety centrifuge cups and rotors Eye and face protection Respiratory protection [BSL-3] 9. Biosafety Level 1IntroductionSuitable for work involving well- characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment. 10. Biosafety Level 1 Introduction Examples: Bacillus subtilis Naegleria gruberi Infectious canine hepatitis virus E. coli 11. Biosafety Level 1 Laboratory Facilities (Secondary Barriers) 12. Biosafety Level 1 Laboratory Facilities (Secondary Barriers) Sink for handwashing Work surfaces easily cleaned Bench tops Sturdy furniture Windows fitted with flyscreens