Guide to the Animal Use Statistics ReportAnimal Welfare Act 2002, Part 2 Use of Animals for Scientific Purposes

Government of Western Australia

01 January 2018 - 31 December 2018

What is the Animal Use Statistics Form?A condition of UWA’s licence to use animals for scientific purposes requires UWA to provide Animal Use Statistics to the State Government, each calendar year, i.e., 1 January to 31 December.This guide is prepared to assist you when completing the Animal Use Statistics Excel spreadsheet.Collecting statisticsAnimals must be counted in each project and must be included each calendar year they are in a project.Only animal use in Western Australia is to be included in the spreadsheet.If no animals used (or supplied) the tables must be returned with ‘0’s in the first line (‘Nil’ return)I only do wildlife observations (no trapping). Do I need to submit the Animal Use Statistics?Observational studies are considered ‘use’ and researchers are required to submit a statistics spreadsheet Use Procedure Code ‘1’ - Observation involving no or minor interference. If the exact number used is unknown for an observational study please provide a general indication of the numbers observed.Procedures CodeProcedure Codes are intended to give an indication of the impact of procedures on the animals used. Examples are listed below: Column 3: Procedure. See also Section 19 of the AEC application approval.Multiple animal species categories and/or procedures in a single AEC projectSome projects will have more than one animal species code (01-113). Examples are listed below in Column 4. Species Categories and some projects will have animals which are subject to different Procedure Codes. In these cases, the projects should be split into appropriate ‘sub-projects’ which each only having one species category and one procedure code and each of these ‘sub-projects’ occupy a single line of the spreadsheet. Each line should have the same AEC project number. Only count animals once per year per project. See Example Animal Use StatisticsStages of developmentThe State Government is seeking data on the use of prepartum or embryonic animals which have reached over half gestation or incubation (e.g. embryonated eggs) but have not been allowed to progress to independent viability (e.g. they are killed before they are born/hatched). Only include embryonic or foetal animals once the development has progressed beyond half the gestation or incubation period for the animal. Stages earlier than this should not be counted (e.g. do not count chicken embryos used for cell culture at 9 days of incubation (Chicken incubation is 21 days)Fish and Cephalopods (e.g. squid, octopus)The reporting of fish and cephalopods is not mandatory under the current WA legislation but it is useful.Re-use of animalsEach year, an animal should be counted once for each project in which it is used. If the project is renewed the following year, then that animal is counted once again in that subsequent year. If an animal is transferred to another project it must be counted twice - once for each project.When an animal has been re-used, this must be noted in the spreadsheet in Column 6 Comments. Production of genetically modified animalsThe production of genetically modified animals may involve a wide range of procedures, making it difficult to assign these to a particular Procedure Code. The production of genetically modified animals is animal “use”. A category has been included for these animals to permit easier collection of data. It effectively includes ALL animals used in genetically modified animal production other than the final progeny.

Column 1: PROJECT NUMBEREnter your AEC approval number for: Research 100/XXXX, Teaching 200/XXX or Breeding 300/XXX

Column 2: PURPOSEEnter the most appropriate numerical code (1-9) from those listed below to describe the primary purpose of the project (one only for each project should be entered).

1 Stock maintenance2A Stock Breeding (genetically modified)2B Stock breeding (not genetically modified)

3 Education: Projects carried out to achieve educational objectives.4 Research: Human or animal biology5 Research: Human or animal health & welfare6 Research: Animal management or production7 Research: Environmental study

COLUMN 3: PROCEDUREEnter the highest appropriate numerical code (1-9) from those listed below to describe the type of procedures carried out on the animals in the project, as indicated in the AEC approvalQ19. Classification of Procedures.

1 Observation involving no or minor interference2 Animal unconscious without recovery3 Minor conscious intervention4 Minor surgery with recovery5 Minor physiological challenge6 Major surgery with recovery7 Major physiological challenge

Death as an end point testing (NB: UWA does not support death as an end point testing)9 Production of genetically modified animals

Column 4: SPECIES CATEGORIES

Enter numerical code (01-113) from those listed below to describe the species category used in the projectNote: Where more than one species category is used in a project, enter additional lines for each species

*Definitions of terms used:Domestic - any animal that has been tamed over a number of generations and has adapted to a human environment.

Native - an animal that occurs naturally in Australia (eg. Kangaroo).

Native Non-endemic – an animal that occurs naturally in Australia but not naturally occurring in WA (eg Koalas).

Native endemic - an animal that occurs naturally in WA (eg. Numbat or Emu).

Poultry – domestic fowls, such as chickens, quails, turkeys, ducks or geese that are commonly raised for meat or eggs.

Exotic - an animal that has been introduced into Australia (eg foxes).

Zoo animals - an animal that is being kept in a zoo. Only include animals that are used for educational purposes.

Species Category Species Category

Laboratory mammals

01 Mouse

Native mammals

50 Macropod02 Rat 51 Possums and gliders03 Guinea Pig 52 Native rats and mice04 Rabbit 53 Dasyurid06 Ferret 54 Wombat07 Other laboratory

mammal (not primates)55 Dingo

Domestic* mammals

10 Sheep 56 Echidna11 Cow 57 Bandicoot12 Pig 58 Bilbie13 Horse 59 Bat14 Goat 60 Other native mammal15 Deer

Non - endemic pest mammals

70 Goat16 Cat 71 Camel17 Dog 72 Cat18 Other domestic

mammal73 Rat

Birds

20 Poultry* 74 Mouse21 Exotic Captive 75 Pig22 Exotic* Wild 76 Rabbit23 Native* Captive 77 Wild Dog (not dingo)24 Native Non-endemic* 78 Cow25 Native endemic* 79 Horse

Aquatic animals

30 Fish (reporting not mandatory)

80 Fox

31 Cephalopod (reporting not mandatory)

81 Other non-endemic pest mammal

32 Whale and dolphin Amphibians 90 Native amphibian33 Dugong 91 Exotic amphibian34 Seal Zoo animals 100 Zoo animal

Reptiles

40 Lizard

Primates

110 Marmoset41 Snake 111 Macaque42 Turtle/Tortoise -

Freshwater112 Baboon

43 Turtle/Tortoise - Marine

113 Other primate

44 Crocodile45 Other reptile

Column 5: NUMBER USEDEnter the number of animals that were used / ordered / generated (from breeding) in the calendar year 1 January – 31 December. Animals must be counted in each project and must be included for each calendar year they are in a long-term project, (i.e., not the total number the AEC approved)

Column 6: COMMENTSUse this column to communicate any other information, eg. use of pre-term animals / culls.

Column 7: PRE-TERM (Y/N)If information is available, indicate whether the animals used in the project are pre-term using:‘Y’ for yes or leave blank for no.

Column 8: SOURCE

Name of Source / Vendor were animals were obtained e.g. Animal Resource Centre, Peel Pork.

If the animals were obtained from UWA, i.e., Animal Care Service (ACS), Ridgefield Farm, Zebra fish breeding facility, use: ‘internal’.

If animals are wild caught e.g. Swan river, Abrolhos etc. use: ‘wild’.

Column 9: CONCLUSION OF USE (this column is optional)This column is to contain information on provisions made for the animal at the conclusion of its use. This may include rehousing, return to natural habitat, humane killing and/or reuse.

The Animal Welfare Act imposes penalties for providing false or misleading information. A licence may be suspended or revoked for providing false or misleading information.

Example Animal Use Statistics

1. Project No. 2. Purpose 3. Procedure

Code4. Species category

5. Number used 6. Comments 7. Pre-term

(Y/N) 8. Source 9. Conclusion of use

100/1240 4 3 01 20 Internal Humanelyeuthanased

100/1240 5 6 01 5 Not used this year but

were allocated to this project

Internal N/A

100/1240 4 5 01 25 Not used this year

Internal N/A