csiro national research flagship - nuffield international
TRANSCRIPT
CSIRO National Research Flagship
Dr Bruce Lee
Director, Food Futures Flagship
Nuffield International Contemporary Scholars Conference
25 February 2008
Melbourne
“..the most promising mechanism yet to drive
large-scale activity addressing National Research Priorities…”
Review of the National Research Flagships 2006
National Research Flagships Program
• A powerful mechanism for addressing National Research Challenges based on world-class science
• We form the very best multidisciplinary teams with our partners from the business and research communities
• We focus on delivery, impact and adoption of research outputs
• Flagships emphasise large scale, long duration, integrated research programs
• Flagship goals are based on deep analysis of challenges and opportunities and how research can contribute to solutions
• The Flagships funding model allow flexible responses to emerging issues through the mechanisms of the CSIRO Science Investment Process and the Flagship Collaboration Fund
Six Flagships (+ 3)
Transforming the international competitiveness of and adding $3B of value to Australian Agrifood
Minerals Down UnderNiche ManufacturingClimate Adaptation
Flagships: A Collaborative Venture
Resources
Processing
Markets
Production
Nutrition
Food Engineeringand processing
Genetics and Breeding – plant & animal
Consumer & sensoryScience & psychology
Tissue Engineering
Food matrix
Materials Science
Maths & Information
ICT
SeparationScience
Biosensing
Marine &Atmospheric
EntomologyPlantIndustry Livestock
Industries
Food ScienceAustralia
Human Nutrition
ICT Centre
Maths & Information Sciences
Land
& Water
Manufacturing & Infrastructure Technology
Molecular & Health Technologies
Materials Science & Engineering
Textile Fibre Technology
Australian Grains Industry under significant
pressure:
• Recent entry of low cost high volume producers.
• Established competitors heavily subsidised
• Constantly rising input costs
�Causing annual erosion in terms of trade 2% per annum
�Countered by annual increase in performance 1.7%
Setting the scene
• Modern starchy processed foods are digested quickly and efficiently in
the small intestine. These foods are low in RS and high GI.
• This means the small intestine is flooded with glucose while the colon
is starved (leading to dysfunction and cancer risk)
• Low digestibility grains containing Resistant Starch are good for health,
having a low glycaemic index and promoting a healthy bowel
• High amylose products are a good source of Resistant Starch
Healthy Grains – Redesigning Starches
High Amylose Wheat
Consumer Driver
0
5
10
15
20
25
30
35
0 10 20 30 40 50
Large bowel cancer incidence (cases/100,000)
Estimated R
S intake (g/day)
Source: Cassidy et al 1994
US
Aust.
Mechanisms for generating starches with increased amylose content
ADPglucose
Amylose
GBSSI
Amylopectin
SSI
SSIIa
SSIII
Isa1
BEI
BEIIa
BEIIb
3. Increase
amylose
synthesis?
1. Decrease
Amylopectin
Synthesis
2. Decrease
Branching
Activity
RNAi Suppression BEII
Genetics: World-first proof-of-concept using RNAi technology via GM*
0
0.5
1
1.5
2
0 50 100 150
Elution volume
Glucose concentration
-1
0
1
2
3
4
5
6
0 50 100 150
Elution volume
Glu
cose c
oncentr
ation
25%Amylose
75%Amylose
Technology being further developed for commercialisation by non- GM mechanism. GM ready to go if regulatory and consumer issues allow.
*Regina et al (2006) Proc Natl Acd Sci USA 103: 3546- 3551
High Amylose Wheat
Nutrition Substantiation: Rat Trial
Feeding High Amylose wheat to rats leads to:
• Increased large bowel digesta
• Reduced caecal pH
• Increased SCFA production
• No deleterious effects
Strong package of gastrointestinal health attributes justifying further trials in large
animals and humans
High Amylose Wheat
High Fibre Grains Research Cluster
• Dietary fibre conventionally characterised as
• ‘insoluble’ [e.g. cellulose, lignin] or
• ‘soluble’ [e.g. pectin, cereal non-starch polysaccharides]
• Two major cereal non-starch polysaccharides (NSP):
arabinoxylan and (1,3;1,4)-β-glucan
= xylose = arabinose
Long Chain PUFAs (Omega-3)
WHY?- Global fish stocks depleted
- Demand for PUFA Health Benefits increasing
Alternate renewable sources: Terrestrial plants
5 CSIRO Divisions3 Universities3 Partners
Health Benefits of Omega-3 Long-Chain PUFA
Microalgae make
long-chain Omega-3
Fish eat
microalgae
We eat
fish
Benefits:
-Cardiovascular
-Brain function
-Inflammatory disease arthritis
LC-Omega-3 PUFA Biosynthesis
18:118:0
Humans
20:5
22:6
22:5
18:2 αααα−−−−18:3Many
Plants
18:4SDASome
Plants
Metabolic Engineering Solutions Required to Bridge the Elongation/Desaturation Gap
Humans
DHA
EPA
Target
Compounds
Proof of Concept Engineering Strategy
Humans
18:118:016:0
18:4 SDA
∆6-des
∆5-elo
∆4-des
∆5-des
∆6-elo
DHA
EPA20:5
20:4
22:6
22:5
18:2 αααα−−−−18:3
Genes
Added
EPA 2.4%
DHA 1%
Consumer Engagement
Key Research Findings
�Communicate anticipated benefits eg: GM/Health
�Consumer preference for GM “one step removed”
�Community and consumer acceptance is paramount in determining the successful adoption of new technologies and novel products in the Agrifood supply chain
Nitrogen Use Efficiency (NUE)
NUE Canola Conventional Canola
No GeneGene
0 50 100 150 200 2501200
1600
2000
2400
2800
3200
3600
4000
4400
Seed yield [lb/ac]
Nitrogen application level [lb N/ac]
Transgenic
Control
1
Arrow #1
Same yield, 66% less N
Arrow #2
33% Higher yield, same N
NUE Canola
2
0 50 100 150 200 2501200
1600
2000
2400
2800
3200
3600
4000
4400
Seed yield [lb/ac]
Nitrogen application level [lb N/ac]
Transgenic
Control
1
Arrow #1
Same yield, 66% less N
Arrow #2
33% Higher yield, same N
NUE Canola
2
NUE Technology Summary
• 50-60% reduction nitrogen fertilizer requirement
• Demonstrated in canola (through 9 field trials over
4 years) as a representative broadleaf crop.
• Cotton, soybeans, sunflower, sugarbeets
• Demonstrated in rice as a representative grass
crop.
• Corn, wheat, barley, oats, sugarcane
0
1
2
3
4
5
6
1 2 3
Value adding goalsBillions of dollars
Livestock Breeds Aquaculture Breeds Aquafeeds
4.8B
0.4B
0.8B
0.8B
100MCurrent value
Added value
Added value
• Improved animal products via
advanced genetics and breeding
technologies
• Increased food exports and
production of healthier and
safer foods
Comparative maturity of Beef & Aquaculture
Production efficiency
Apply advanced genetic technology to
enhance the rate of progress
Health
Product
quality
Environment
NutritionGenetics
Time (Years)
Breed Engineering
Bos indicus (recipient)Inferior meat quality thrives
in tropical Northern Australia
Bos taurus (donor)
superior meat quality poor survival in
tropical Northern Australia
Improved growth rate
yield and meat quality
Germ cell transfer in cattle
Global aquaculture and the novel aquafeeds opportunity
• Aquaculture has been the fastest growing food-producing sector in the world since the 1950s
0 2 4 6 8 10
Cattle meat
Wild fish
Population
Sheep meat
Pig meat
Fruit & veges
Poultry meat
Aquaculture
Annual production increase (%)
8.9
4.9
3.4
3.0
2.0
1.3
1.2
1.0
Cereal grain 1.5
Aquaculture Breed Enhancement
• Selective breeding
• Molecular genetics
• Virology & health
• Reproductive sterility
• Food quality
CMAR, CLI, FSA
Prawns
Partners: APFA, FRDC, GCMA, Seafarm Pty Ltd, AIMS, QDPIF
• Selective breeding
• Molecular genetics
• Sex pre- selection
• Health • Vaccine development
• Disease resistance
CMAR, CLI, CMIS
Atlantic salmon
Partners: Aquafin CRC, FRDC, TSGA, SALTAS, Tassal Group, HuonAquaculture
• Selective breeding
• Molecular genetics
• Reproductive sterility
• Food quality
CMAR, CLI, FSA
Partners: Tasmanian Selected Abalone,Abalone Aquaculture, Abalone Farms Aust., Aust. Ocean Biotechnology, Cold Gold
Abalone
Develop and apply genetic technology to produce healthy, genetically superior strains of key aquaculture species with enhanced production efficiency, profitability and market penetration
Global opportunities
• A key opportunity for Australia is as a seed-stock, genetic technology and aquafeeds supplier to the global aquaculture industry – key focus on prawns & aquafeeds
China
Indonesia
Vietnam Thailand
India
Australia
Current projects
Expressions of interest
Naturally Structured Foods
and Bioactives
Concentrated Proteins
Energy
Controlled
Foods
New
Healthier
Foods
Scouting for new ideas and
scientific breakthroughs
Theme Structure
Stream 1
Stream 2
Stream 3
Bioactives
Innovative Ingredients and Food
Theme Goal
$700M by 2013
Novel Separation Technologies9 Bioactives
WHY?•Value add to waste•Health Benefits
Dermatan Sulphate (DS)
Anti-thrombin activity
0
0.05
0.1
0.15
0.2
0.25
0.3
0 5 10 15 20 25 30 35 40 45
Time in minutes
A405nm
HC11
Throm
Trial 5
Sigma CSB
Anti-thrombin activity
• Consistent and
predictable style and
quality
• Avoiding taints,
contaminants and off
flavours
• Maximising price point
• From a raw material that
is far from consistent
using a biological
process that is variable
Quality Biosensors
Winemakers want to be able to make wine to a precise flavour and aroma specification
BmOr22 NDBmOr17
BmOr8 *BmOr21HvCr19
BmOr20HvCr21BmOr19 ****BmOr12 ****
BmOr15 ***BmOr13 ***
HvCr8BmOr29 ND
BmOr27 ** BmOr36 **
BmOr4 **BmOr9 **
HvCr13BmOr1 ****
BmOr5 ***BmOr7HvCr14HvCr15HvCr16HvCr6
HvCr11BmOr3 ****
BmOr6 ***BmOr40 **
BmOr24 *BmOr25HvCr12BmOr11 **
HvCr7 BmOr23
HvCr9BmOr42
BmOr32 NDBmOr14 *
HvCr20BmOr28 *
HvCr18BmOr33 *** BmOr34 ***BmOr30 **
BmOr37 NDBmOr39 ND
BmOr38 NDBmOr35 ***
BmOr16 **BmOr26 *HvCr17
HvCr10BmOr31 *
BmOr41 **BmOr10 ***
BmOr18 **HvCr3
BmOr2 ****SeOr2HvOr2AiOr2
0.5 Changes
Male Biased(exceptions areBmOr9 & HvCr6 )
99
100
100
100
69
89
99
100
97
100
100
100
100
100
100
100
100
91
Female Biased
Female Biased
Novel receptors identified by CSIRO
Biosensor Research Cluster
• Control flavour and aroma more precisely• deliver a consistent style of wine
• even out the variations between grape lots, seasons and localities
• maximise the price point for every crush and every bottle
• Developing Cybernose to establish the link between viticulture and flavour potentialof grapes
Partners: GWRDC, Wingara Group, Fosters Group, Partners: GWRDC, Wingara Group, Fosters Group,
Yalumba, Orlando WyndhamYalumba, Orlando Wyndham
Flagship Collaboration Cluster