Gene Therapy—the Application of Adenovirus Vectors

Prepared by Creative Biogene

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• In terms of genetic defect, abnormal genome is the bad source. If we could find the exact abnormal part and fix it, then the illness could be avoided, at least theoretically.

 • With thinking the same, many

attempts have been made to treat the disorders by adding back the correct genetic information to individual cells. This is what we are going to talk today—gene therapy by adding foreign genetic material to treat disorders under this setting.

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• Dating back to 1970s the conceptual emerging, gene therapy trials used to treat human disorders actually really started after twenty years. However like every new thing stumbles, these initial trials did not add color to this exciting and promising field, might because of short of background and related knowledge. So the results at that time were initial dramatic clinical failures.

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• With the development of technology and constant accumulation of understanding, people begun to think about an alternate—if we want the gene therapy to work, the correct genetic information has to be packaged and delivered to target cells, but how?

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The answer is adenovirus vectors. Adenoviral packaging vectors are attractive options for its unique features:

• Low pathogenicity, • High viral yields, • Carry large genes, and • Have transgene specific B

and T cell responses

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• In addition to this, when packaged with recognizable antigens from various diseases, adenovirus vectors may serve as vaccines. Novel vaccines targeting complex pathogens like HIV, HCV, TB, malaria, influenza as well as cancer is a long-standing goal of human gene therapy.

• A team of scientists from Creative Biogene has developed exclusive techniques in adenovirus vector design and construction, as well as the generation of the adenovirus in high titer. With unique processes for the generation of new adenovirus constructs, Creative Biogene can provide the fastest turn-around time of any supplier in the industry.

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The services include:   • Clone gene of interest into

appropriate transfer vector. • Transfect HEK 293 cells with recombinant viral DNA to produce recombinant adenoviruses.  • Create high-titer recombinant adenovirus stock.

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Contact to order• USA

45-1 Ramsey Road, Shirley, NY 11967, USATel: 1-631-626-9181Email： info@creative-biogene.comUnited Kingdom St John's Innovation Centre, Cowley Road, Cambridge CB4 0WS, UK Tel: 44-207-048-3343 Email： info@creative-biogene.com

• Web: http://www.creative-biogene.com/Services/Custom-Viral-Service/Adenovirus-service.html