chemosensory perception we taste what we smell? taylor.pdf · 2017-05-24 · •triangle test...
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UCD Institute of Food & Health1
Chemosensory perceptionWe taste what we smell?
Andy Taylor
Flavometrix Limited
Loughborough, UK
May 17 2017
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Title is a generalisation!
UCD Institute of Food & Health 2
Difficult to differentiate apple and
onion when wearing noseclips
Key differentiator is mouthfeel
due to different particle size
Odour of cheese changes
significantly when in mouth
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UCD Institute of Food & Health 3
Definition of flavour
Sound
Appearance
& colour
Taste
Smell
Mouthfeel
Texture
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UCD Institute of Food & Health 4
How are the senses stimulated?
Tasty
Ortho and
retronasal
aroma routes
Taste buds on
tongue
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UCD Institute of Food & Health 5
How can we measure flavour stimuli?
• Sensory is the ultimate test for acceptability
• Instrumental gives qualitative and quantitative data but not sensory impact
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Sensory-instrumental
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Productperceptualdifferences
Quantitative data
Identifycomponents
causing change
Sensory
attributes
attributes
Instrumental
Detailed composition
Individual components
Sensory + Instrumental
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UCD Institute of Food & Health 7
Measuring the flavour profile that reaches the receptors during eating
Aroma release during eating APCI-MS & PTR-MS
Tastant release during eating;
sample in mouth; off-line MS
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UCD Institute of Food & Health 8
Does in-nose measurement
correlate with perception?
15.20 15.40 15.60 15.80 16.00 16.20 16.40 16.60 16.80Time0
100
%
TTR34 Unused
ANALOG
8.10e5
Height
Black line
Time-intensity sensory
perception
Red and pink
Breath by breath
aroma release
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UCD Institute of Food & Health 9
Applications of science approach to real foods
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UCD Institute of Food & Health 10
Effect of fat content on aroma release during eating in yogurt
0
100000
200000
300000
400000
500000
600000
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6
Time (min)
Inte
nsit
y
0% Fat
3.5% Fat
10% Fat
Each yogurt contains the
same amount of
flavour
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UCD Institute of Food & Health 11
Shape of aroma release with different fat contents
0.0
20.0
40.0
60.0
80.0
100.0
120.0
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6
Time (min)
% Im
ax
0% Fat
3.5% Fat
10% Fat
Zero fat
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UCD Institute of Food & Health 12
Sensory data on yogurts
• Triangle test analysis (30 panellists)
Test Assessors Perceiving Difference
Significance Positively
Sure of Choice
Unsure Of Choice
Fat free vs
Higher fat 22 < 0.0001 20 2
Fat free + -CD vs
Higher fat
15 0.0435 8 7
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UCD Institute of Food & Health 13
Fat and flavoured milk
Fat content of milk• Changes partition of aromas
– Amount and rate of aroma release affected
• Changes viscosity of product• Changes mouthfeel of food• Which stimuli are significant?• Hypothesis: aroma signal most important
so if reformulation gives same aroma stimuli, perceived flavour is the same
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UCD Institute of Food & Health 14
Aroma release in regular & low fat flavoured milks – same aroma content
0 4 8 12
Lipid Effect
0
25
50
75
100
Fre
qu
ency
a
-0.5 0.0 0.5 1.0 1.5
Log Lipid Effect
0
15
30
Fre
quen
cy
b
0 4 8 12
Lipid Effect
0
25
50
75
100
Fre
qu
ency
a
-0.5 0.0 0.5 1.0 1.5
Log Lipid Effect
0
15
30
Fre
quen
cy
b
Result
Most values above 0 so greater release from low
fat milks (expected)
Can calculate mean release
18 instances of log LE less than 0 were
random
May 17 2017
90 panellists
duplicate samples
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UCD Institute of Food & Health 15
Aroma release in regular & low fat flavoured milks – adjusted aroma content
-0.50 0.00 0.50
Log Lipid Effect
0
15
30
45
Fre
qu
en
cy
Result
Mean value for log LE lies at 0 – no
statistical difference in aroma release when
aroma content adjusted
May 17 2017
Shojaei et al., 2006, IJFST 41, 1192
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UCD Institute of Food & Health 16
Sensory testing – statistics
Replicate
Number
Number of
panelists
LF>RF a Significant
b
1 98 80 <0.0001
2 98 80 <0.0001
3 98 88 <0.0001
4 98 85 <0.0001
1
Replicate
Number
Number of
panelists
LF>RF
a
Significant
b
1 90 41 NS c
2 90 51 NS
3 90 44 NS
4 90 43 NS
1
Same aroma content Adjusted aroma content
Forced choice difference test, LF>RF means low fat rated as higher aroma intensity than regular fat; significance by t-test;
panelists who got it wrong were randomMay 17 2017
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STUDYING TASTE-AROMA INTERACTIONS
UCD Institute of Food & Health 17May 17 2017
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UCD Institute of Food & Health 18
Chewing gum dragees: Aroma and sugar release & sensory TI
0
50
100
150
200
250
300
0 1 2 3 4 5 6
Time (min)
Inte
ns
ity
Aroma TR
Sugar
In - Mouth
Sensory TI
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UCD Institute of Food & Health 19
Stick chewing gum: Aroma and sugar release & sensory TI
0
100
200
300
400
500
600
700
800
0 1 2 3 4 5 6
Time (min)
Inte
nsit
y
Aroma TR
Sensory TI
Sugar
In - Mouth
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Commercial applications
UCD Institute of Food & Health 20
Taste Tech
Prolonging gum flavor
Advert April 2017
Stride gum
“Ridiculously long lasting flavor”
Cadbury Adams
2006
May 17 2017
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Other interactions
UCD Institute of Food & Health 21
ViscosityAffects taste release rather than aroma
release
EthanolComplex interactions with interfacial
layer, partition and proteins
Acid/sugar/fruit aroma
Contour plots indicate ratios of sugar
and acid that give equal flavour with
fixed aroma content
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BRAIN IMAGINGMEASURING INTERACTIONS AT THE COGNITIVE LEVEL
UCD Institute of Food & Health 22May 17 2017
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Set-up of subjects and scanner
May 17 2017 UCD Institute of Food & Health 23
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Aroma delivery during fMRI paradigm
May 17 2017 UCD Institute of Food & Health 24
APcI-MS
Aroma delivery
Delivery system
Interface
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Time courses at 2 different voxels
May 17 2017 UCD Institute of Food & Health 25
-0.2
0
0.2
0.4
0.6
0 5 10 15 20
Time (s)
% s
ignal c
hange
Right anterior insula (taste)Left superior insula (aroma)
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fMRI results
May 17 2017 UCD Institute of Food & Health 26
Cortical activation n=11
taste+aroma > unimodal aroma + unimodal taste
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Future?
UCD Institute of Food & Health 27
Current view of flavourHedonics
Developing view of flavour
Mouth to brain to gut to metabolism
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UCD Institute of Food & Health 28
Acknowledgements
Flavometrix -University of NottinghamZahra Shojaei, Rob Linforth, Avinash Kant, Jim Davidson, Joanne Hort, David Cook, Tracey Hollowood, Michael Brauss, Jamie Langridge
Flavometrix works in partnership with Nichirei Corporation, Japan to solve commercial flavour problems
May 17 2017