chapter 8 an introduction to metabolism. overview: the energy of life the living cell is a miniature...
TRANSCRIPT
Chapter 8
An Introduction to Metabolism
Overview: The Energy of Life
• The living cell is a miniature chemical factory where thousands of reactions occur
• The cell extracts energy and applies energy to perform work
• Some organisms even convert energy to light, as in bioluminescence
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
Metabolism
• Metabolism is the entire collection of an organism’s chemical reactions
• A metabolic pathway begins with a specific molecule and ends with a product
• Each step is catalyzed by a specific enzyme
Enzyme 1 Enzyme 2 Enzyme 3
DCBAReaction 1 Reaction 3Reaction 2
Startingmolecule
Product
Two Types of Metabolism
• Catabolic pathways release energy by breaking down complex molecules into simpler compounds
GLUCOSE CO2 + H2O + ATP
• Anabolic pathways consume energy to build complex molecules from simpler ones
Amino acids Proteins
Energy
• Energy is the capacity to cause change• Types of energy
– Kinetic energy is energy associated with motion
– Potential energy is energy that matter possesses because of its location or structure
• Energy can be converted from one form to another
Fig. 8-2
Climbing up converts the kineticenergy of muscle movementto potential energy.
A diver has less potentialenergy in the waterthan on the platform.
Diving convertspotential energy tokinetic energy.
A diver has more potentialenergy on the platformthan in the water.
The Laws of Energy Transformation
• Thermodynamics is the study of energy transformations
• First law of thermodynamics
– Energy can be transferred and transformed, but it cannot be created or destroyed
• Second law of thermodynamics
– During every energy transfer or transformation, some energy is unusable, and is often lost as heat (entropy)
Free Energy• Free Energy (∆G) - measures a system’s energy
that is available to perform work.
∆G = ∆H – T∆S
• Spontaneous
– a process that can occur without the input of energy; ∆G must be a negative value
• Nonspontaneous
– a process that requires the input of energy; ∆G will be a positive value or zero
As Biologists, we want to know which rxns of life are spontaneous. These rxns canbe harnessed to do work (to drive the nonspontaneous rxns.
When we apply the free energy concept to processes of life and with respect to metabolism, we describe rxns as exergonic or endergonic.
• An exergonic reaction proceeds with a net release of free energy and is spontaneous
Glucose + O2 CO2 + H2O ∆G = -686 kcal/mol
• An endergonic reaction absorbs free energy from its surroundings and is nonspontaneous
CO2 + H2O Glucose + O2 ∆G = +686 kcal/mol
3 Main Types of Cellular Work
• Mechanical
• Transport
• Chemical
Fig. 8-8
Phosphate groupsRibose
Adenine
Fig. 8-9
Inorganic phosphate
Energy
Adenosine triphosphate (ATP)
Adenosine diphosphate (ADP)
P P
P P P
P ++
H2O
i
Fig. 8-11
(b) Mechanical work: ATP binds noncovalently to motor proteins, then is hydrolyzed
Membrane protein
Pi
ADP+
P
Solute Solute transported
Pi
Vesicle Cytoskeletal track
Motor protein Protein moved
(a) Transport work: ATP phosphorylates transport proteins
ATP
ATP
Fig. 8-12
P iADP +
Energy fromcatabolism (exergonic,energy-releasingprocesses)
Energy for cellularwork (endergonic,energy-consumingprocesses)
ATP + H2O
Fig. 8-14
Progress of the reaction
Products
Reactants
∆G < O
Transition state
Fre
e en
erg
y EA
DC
BA
D
D
C
C
B
B
A
A
Enzyme Inhibitors