biotecnology journal

7/29/2019 Biotecnology Journal

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Updated: June 2013

Biotechnology Journal

Instructions to Authors

Manuscript submission:

http://mc.manuscriptcentral.com/btj

Contact the Editorial Office at:[email protected]

Authors are requested to follow these Instructions carefully. Manuscripts not prepared accordingly

will be returned to authors and this will inevitably lead to a delay in the editorial processing of the

manuscript. Please refer to our Appendix B for Authors to comply with all requirements.

1. Terms of publication ........................................................................................................................ 2

2. Manuscript preparation .................................................................................................................. 5

3. Online submission of manuscripts ................................................................................................ 13

4. Manuscript publication ................................................................................................................. 14

Appendix A: BTJ Keywords .................................................................................................................... 16

Appendix B: Checklist for Authors ......................................................................................................... 17
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www.biotechnology-journal.com

Manuscript submission: http://mc.manuscriptcentral.com/btj

Contact the Editorial Office at: [email protected]

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1. Terms of publicationAll scientific contributions will be peer-reviewed on the criteria of originality and quality. Authors

may suggest up to five potential referees, including their e-mail addresses, as well as individuals

whom they wish to be excluded from the review process. The reason for excluding certain refereesshould be stated. Upon acceptance, papers may be subjected to editorial changes. A revised paper

will retain its original date of receipt only if it is resubmitted to the Editors within three months after

revision was requested. Responsibility for the factual accuracy of a paper rests entirely with the

author.

Upon acceptance of the manuscript the corresponding author is required to fill in the Copyright

Transfer Agreement, sign it and submit it to the Editorial Office.

1.1. Aims and scopeBiotechnology Journal (BTJ) is an international resource for both biotechnology researchers and

professionals in related disciplines. Fully comprehensive in its scope, the Journal publishes strictly

peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Special

attention is also paid to the public, legal, ethical and cultural aspects of biotechnological research in

the Forum section. Some issues are devoted to a special topic, providing the latest comprehensive

information on the most crucial areas of research and technological advances. In addition to special

issues, the journal welcomes unsolicited submissions both for primary research articles and for

review articles (see 4) for additional review article).

The Journal contains original papers in the form of Research Articles, Rapid Communications and

Technical Reports, illustrating the latest research results. BTJ welcomes submission of papers in areasincluding DNA/protein engineering, all -omics fields (genomics, proteomics, metabolomics, systems

biology etc.), bioinformatics (algorithms and modeling), imaging, analytical biotech

(sensors/detectors for analytes/macromolecules), plant/agricultural, food or environmental

biotechnology, health and therapeutical biotechnology (including antisense/siRNAs, enzymes,

peptide, etc.), regenerative medicine (stem cells, tissue engineering and biomaterials), translational

immunology (antibody engineering, xenotransplantation, T-cell therapies), and biosafety or

biosecurity.

BTJ promotes a special emphasis on:

Systems Biotechnology

High-Throughput and Microarray Technologies Synthetic Biology and Metabolic Engineering

Nanobiotechnology

Medical Biotechnology

Translational Biotechnology and Applied Microbiology

Methods and Advances

Biosafety, Biotech Ethics, Science Communication
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In particular, BTJ welcomes submissions on

Systems Biology/Bioinformatics: Overview and interplay between biological system components and

their functional implications (e.g. enzymes and metabolites in a metabolic pathway). Computational

analysis of all topics mentioned below (modeling, algorithms, data deconvolution).

Synthetic Biology: The design and construction of new biological parts, devices and systems; re-

design of natural biological systems for useful purposes.

Nanobiotech/ Biomaterials: Nanomaterials for industrial (white) biotech, drug delivery therapeutics,

biochips, micro- and nanofluidics, nanosensors, and nanosystems for imaging; engineering materials

for biological application, molecular imprinting, and biomimetics.

Medicine/ Red Biotech/ Stem cells: Therapeutic cloning (somatic cell nuclear transfer), tissue

engineering, xenotransplantation; DNA or RNA therapeutics (gene therapy, targeting,

immunogenicity); vaccines and applied immunology (Ab engineering, T-cell therapies, therapies

exploiting innate immunity, antigen delivery vectors and approaches; viral or non-viral strategies for

drug delivery.

Mammalian Biotech/Genetics: Manipulating gene structure and control of gene expression;

transgenic animals, knockouts, reproductive cloning, biopharmaceutical and enzyme production,

transgene targeting and expression strategies.

Metabolic Engineering: Genetic manipulation of species of interest to modify or allow the

production of a commercially or therapeutically relevant compound.

Biocatalysis: Evolution and protein design (antibodies, enzymes, drugs, etc.)

White/Industrial Biotechnology: Chemical synthesis by bacteria, yeast or other organisms, use of

enzymes as industrial catalysts, biopharmaceutics, biofuels.

Omics, Analytical Biotech, Biosensors: (Meta)genomics, structural genomics, gene function analysis

(e.g. arrays, SAGE), pharmacogenomics (SNPs), gene structure analysis, chemogenomics, proteomics

(e.g. mass spectrometry, yeast two hybrid, arrays and chips, NMR, SPR), metabolomics

(chromatography, mass spectrometry). Analytical methods and systems for monitoring research,

development and production of biotechnological products.

Methods: Technical aspects and practical protocols for all biotech applications; expression systems in

mammalian, insect, bacterial, fungal or plant cells; screening methods; novel

organism/cell/molecular imaging techniques; high-throughput imaging tools for drug screening,

fluorescence microscopy, electron microscopy/tomography, confocal imaging, etc.

Biochemical Engineering/ Downstream Processing: Chemical engineering aspects of biological

processes from primary material to product recovery; separation, purification, and packaging after

fermentation or bioconversion.

Plant Biotechnology: Crop improvement (resistance to stress, disease, pests), nutraceuticals, forest

biotech, plant vaccines or bioreactors, biosecurity and gene-containment strategies, bioenergy,

microbial biodegradation, food biotech, environmental biotech: bioremediation, biomining,

phytoremediation, monitoring.

Food Biotechnology: Application of microbiology and metabolic aspects to food and beverage

systems; food safety and pathogens, food fermentation, food bioactive compounds.


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Biosafety, Biotech Ethics, Science Communication

Out of scope

Biotechnology Journal will NOT consider submissions consisting of marginal advancements, for

example cloning without functional relevance, characterizations without functional data orapplications, fermentation optimizations without technical novelty, adsorption and chromatography

studies without a deeper insight into the process, or separation studies without a modeling support.

In silico predictions with no supporting experimental data or implications for further studies will also

not be considered.

Readership

Including, but not limited to: biotechnologists, microbiologists, bioengineers, medical and

pharmaceutical scientists, food and nutrition scientists, agriculturists, environmental scientists,

patent lawyers, and public authorities.

1.2. Publication Ethics1.2.1. Ethical misconductThis Journal endorses the COPE (Committee on Publication Ethics) guidelines and will pursue cases of

suspected research and publication misconduct. In such cases, the Journal will follow the processes

set out by COPE. For more information about COPE please visit the COPE website at

http://www.publicationethics.org.uk. All instances of publishing misconduct, including, but not

limited to, plagiarism, data fabrication, image/data manipulation to falsify/ enhance results, etc. will

result in rejection/retraction of the manuscript.

It is the authors responsibility to obtain permissions for reproduction of figures, tables, or text from

published works. In many cases, the publisher of a journal or book is the copyright owner from whom

the written permission must be obtained. Permission from authors is also encouraged as a

professional courtesy.

1.2.2. Sharing of materialAll materials and reagents that are not commercially available (antibodies, cell lines, constructs, etc.)

and associated protocols detailed in manuscripts published in Biotechnology Journalare to be freelyavailable to academic researchers in a timely manner upon request. The authors agree to this

condition by submitting a manuscript to Biotechnology journal.
http://www.publicationethics.org.uk/http://www.publicationethics.org.uk/


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2. Manuscript preparation2.1. Language assistanceBiotechnology Journalpublishes articles in English. American spelling is preferred. Manuscripts mustbe grammatically and linguistically correct, and authors less familiar with English usage are advised to

seek the help of English-speaking colleagues. These authors may also choose to have their

manuscript professionally edited prior to submission. Help and suggestions for language services can

be found at http://authorservices.wiley.com/bauthor/english_language.asp.

2.2. Types of contributions2.2.1. Scientific contributionsFour categories ofscientific contributions are published in Biotechnology Journal:

1) Research Articles

2) Rapid Communications

3) Technical Reports

4) Review Articles

Research Articles describe complete investigations. Original research articles should not exceed 6500

words; this limit includes references, figure legends and tables. Longer manuscripts will be

considered but only if the increased length is a reflection of the amount of data presented and not a

result of inclusion of unnecessary information. Manuscripts may not have been published previously,

except in the form of a preliminary communication such as meeting abstracts. Research Articles

should be divided into the following sections: Introduction, Materials and methods, Results,

Discussion,Acknowledgementand References. We recommend a maximum of five display elements

(figures/tables).

Rapid Communications describe the latest biotechnological discovery in a brief and succinct form.

These manuscripts should bear the words Rapid Communication immediately above the title on

the first page. Rapid communications should not exceed 2500 words (including references as well as

figure and table legends) and contain no more than three display elements (figures and tables). Rapid

Communications should be divided into the following sections: Introduction, Materials and methods,

Results, Discussion,Acknowledgementand References.

Technical Reports describe the development of a novel method or an improvement or noteworthymodification of an already existing technique or platform used in biotechnology. These manuscripts

should bear the words Technical Report immediately above the title on the first page. Technical

Reports should not exceed 4000 words (including references as well as figure and table legends) and

contain no more than three display elements (figures and tables). Technical Reports should be

divided into the following sections: Introduction, Materials and methods, Results, Discussion,

Acknowledgementand References.

Reviews Articles are to be invited by the Editor. Authors planning to submit unsolicited reviews

should send a brief outline of its contents to the Editorial Office([email protected])prior to writing the

full article. The articles should be divided into sections that are appropriate to the topic. Authors

should also submit a short biography of the corresponding and optionally, one additional author (up
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to 100 words each) accompanied by a portrait image (TIFF or JPEG file). There are two types of

reviews:

Reviews (full-length) should be reserved for subject areas that have not been previously

reviewed in detail. Full-length reviews should not exceed 8000 words (including references

and figure/table legends).

Mini-Reviews provide an update of the latest research. Authors should refer to previous

reviews for detailed background of the subject area. Mini-reviews should not exceed 4000

words (including references and figure/table legends).

For both review types, the article should provide novel insights and synthesis of available literature

within the topic of the review; reviews SHOULD NOT be a mere listing of available information;

reviews should provide a balanced view of the field by citing and discussing relevant data. Authors

are also encouraged to raise controversial opinions - these however, must be supported by relevant

literature and NOT the author's unpublished data. Authors are encouraged to avoid excessive

reference to their own unpublished data. As the peer-review process of Reviews do not allow for

detailed scrutiny of research data, Review articles must not contain previously unpublished data.

IMPORTANT: due to rising printing costs, BTJ must levy page charges on lengthy manuscripts

manuscripts that exceed the above limitations by more than 25% will be requested to cover the costs

of the extra pages. We appreciate the authors understanding in the matter.

2.2.2. Other contributionsBiotechnology journalalso accepts Other contributions of the following types:

Commentaries describe the latest research results published in the same issue of

Biotechnology Journal. These articles are invited by the Editors. Commentaries discuss the

studies in light of current knowledge. Recommended word limit is 2000 words (including

references and figure/table legends); authors are also encouraged to submit summary

figures that support the thesis of the Commentary.

Forum Articles describe the latest news and events in biotechnology, and also relevant

ethical and socioeconomic issues. Examples include:

- Important publications and awards

-Institute or company highlights

- New trends or policies

- Meetings reports

- Industry highlights

- Ethical issues surrounding biotechnology

- Biotech around the world

Please contact the Editorial Office ([email protected])before writing or submitting a contribution to

the BTJ Forum.
mailto:[email protected]:[email protected]:[email protected]:[email protected]


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2.3. Cover letterThe manuscript should be accompanied by a letter of transmittal that includes the following

statements:

1) Why the paper is suitable for publication in Biotechnology Journal?

2) All authors concur with the submission and have seen a draft copy of the manuscript and

agree with its publication.

3) The work has not been published elsewhere, either completely, in part, or in another form.

4) The manuscript has not been submitted to another journal and will not be published

elsewhere within one year after its publication in this journal. Publication in any reasonably

retrievable source constitutes prior publication. Meeting abstracts or preprints do not

constitute prior publication.

5) Conflict of Interest statement: authors are responsible for disclosing all financial and

personal relationships between themselves and others that might bias their work. To prevent

ambiguity, authors must state explicitly whether potential conflicts do or do not exist. Thisstatement will not influence the decision. Intentional omission of any potential conflict of

interest may however, negatively impact the decision to publish the manuscript.

6) The manuscript does/does not contain experiments using animals. The permission of the

national or local authorities (giving the permission or the accreditation no. of the laboratory

and of the investigator) should be stated if animal experiments are included. If no such rules

or permissions have been implicated in the particular country, this must be stated.

7) The manuscript does/does not contain human studies. If such studies are included, it should

be stated that local Ethical Committee approval was received for the studies and that the

informed consent of all participating subjects was obtained. Permission statements relating

to points 4 and 5 should also be included in the text of the manuscript.

Permission statements relating to points 6) and 7) should also be included in the text of the

manuscript.

2.4. Format and style of manuscripts2.4.1. Title pageThe first page of all manuscripts should contain only the following:

i. Title of the paper - containing only the most important keywords pertaining to the subject

matter.ii. Full names (including full first name, middle initial/s, and full family name) of the authors and

the name of their institute(s). If the publication originates from several institutes the

affiliations of all authors should be clearly stated by using superscript numbers after the

name and before the institute.

iii. Name, title and full postal address of the author to whom all correspondence (including

galley proofs) is to be sent. This should also include the e-mail address.

iv. Keywords: provide a list of 3-5 keywords

v. A list of abbreviations used in the paper. Keeping in mind that abbreviations may be a

hindrance to those not familiar with the field of study. Therefore, abbreviations should be

restricted to a minimum. Abbreviations should be introduced only when repeatedly used.

Abbreviations used only in a table or a figure may be defined in the legend.


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2.4.2. AbstractAn Abstract of the manuscript should be included. This should provide a brief background to the

study rationale; present the hypothesis; highlight significant findings/results; and emphasize the

take-home message of the study. This must be self-explanatory and intelligible without reference tothe main text. It should not exceed 200 words. The abstract should not contain any references.

2.4.3. Practical applicationsWhen submitting your manuscript the submission system will ask you to provide the Practical

Applications of your work. The limit is 150 words. This text should highlight the uses either actual

or potential of the research you are presenting. Providing this information will ensure the content

is widely accessible by both academic and corporate audiences. This text should also be included on

the second page of the manuscript. This does not apply to Reviews and Other contributions.

2.4.4.

Guidelines to manuscript sections

Introduction

The Introduction section should contain a description of the problem under investigation and a brief

survey of the existing literature on the subject. The study rationale/hypothesis should also be clearly

described.

Materials and methods

The Materials and Methods section should describe all materials and methods used in the study in

sufficient detail so that an independent, trained reader may repeat the studies. Any materials and

experiments that are used only in the Supporting Information, do not need to be described in the

main text and instead should be included in the Supporting Information. Authors are encouraged to

refer to previously published descriptions of methods and provide only a brief summary of the

method. Any modifications to published methods should be described in detail. Sources (name and

location) of reagents and equipment should be provided.

Results

The Results section describes the main experimental finding of the study. Sub-sections should be

provided to reflect the key messages of the study. Full statistical analysis should be performed. The

name of each test used and its outcome should be detailed, as should the number of samples,

replicates and data presentation (SD vs SEM).

Discussion

The Discussion section describes the findings in light of current knowledge. It should enrich, but not

repeat, the Introduction and the Results sections.

Concluding remarks

The Concluding Remarks section is only applicable to Rapid Communications and Technical Reports,

in which the Results and Discussion sections are combined. Concluding Remarks should provide the

take-home message in a short and succinct manner.


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Acknowledgements

Acknowledgements should be provided on a separate page at the end of the text (before the

References). ALL funding sources for the study and each authors contribution to the article should be

stipulated.

Conflict-of-interest statement

Authors are responsible for disclosing all financial and personal relationships between themselves

and others that might bias their work (e.g. an author writing on advances in basic research on

antibiotics might be a paid consultant, or external contractor, to a biotech or pharmaceutical

company). To prevent ambiguity, authors must state explicitly whether potential conflicts do or do

not exist. At the time of first submission a statement about any conflict of interest MUST be included

in the cover letter and in the manuscript after theAcknowledgements section.

Either: The authors declare no commercial or financial conflict of interest

Or: Conflict of interest: Nature of the conflict of interest, e.g. "Author X is a paid consultant forcompany Y."

This statement will not influence the peer review and the decision on the manuscript. Intentional

omission of any potential conflict of interest however, may negatively impact the decision to publish

the manuscript.

References

References, including those in tables and figure legends, should be numbered sequentially in the

order in which they appear in the text. The numbers should be set in square brackets in the text i.e.

[2, 18]. References are to be collected in numerical order at the end of the manuscript under the

heading References, they should also be typed with double spacing throughout. Titles of journals

should be abbreviated according to the practice ofPubMed. The abbreviated journal title and the

volume number should be in italics.

If necessary, cite unpublished or personal workin the text but do not include it in the reference list.

Articles that have been submitted but not yet accepted should NOT be included in the reference list.

The DOI for the reference should be included at the end of the reference, if no print reference is

available. Abstracts and posters in meeting books must not be cited unless they are generally

accessible. Please note that website addresses must not be included as a reference but should be

inserted in the text directly after the data to which they refer.
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Please note the following examples:

Journals:

[1] Liebeton, K., Eck, J., Identification and

Expression in E. coliof novel nitrile hydratasesfrom the metagenome. Eng. Life Sci. 2004, 4,

557562.

[2] Schmoekel, F. E.,Weber, J. C., Schense, J. C.,

Gratz, K.W. et al., Bone repair with a form of

BMP-2 engineered for incorporation into fibrin

cell ingrowth matrices. Biotechnol. Bioeng. 2005,

DOI: 10.1002/bit.20168.

Books:

[3] Geis, A., Perspectives of genetic engineering

of bacteria used in food fermentations, in:Heller,K. J. (Ed.), Genetically Engineered Food

Methods and Detection, Wiley-VCH Verlag,

Weinheim 2003, pp. 100118.

[4] Chrisou, P., Klee, H. (Ed.), Handbook of Plant

Biotechnology, John Wiley & Sons, 2004

Patents:

[5] Pandey, R., Ratnani, K., Ahmed, S.,Williams, J.,

Direct conversion of methane to hythane.

US Patent 5516967, 1996.

[6] Nadeau, E., Fairbrother J., Use of live bacteriafor growth promotion in animals. US Patent

Application 2007/0218035 A1.

Note that

Papers with multiple authors should be limited to listing the first four authors,

followed by et al.

Papers published online in advance of print should be cited with their DOI.

Other serial publications should be cited in the same manner as journals.

Responsibility for the accuracy of bibliographic references rests entirely with the author.

Tables

Tables with suitable captions at the top and numbered with Arabic numerals should be collected at

the end of the text on separate pages for different tables. Column headings should be kept as brief as

possible and indicate units. Footnotes to tables should be indicated with a), b), c), etc. and typed at

the bottom of the Table.

Figures and legends

Figures should be numbered in the order of their appearance in the text with Arabic numerals. Each

Figure must have a separate legend, which should be self-explanatory and allow readers tounderstand the data presented without reference to the main text. The legends should not appear

under the figures, but be gathered in a separate section (Figure legends) after the references and

tables. The figures should be submitted separately and not included into the main document. The

responsibility for obtaining permissions to reprint Figures and Tables and any associated costs rests

entirely with the author.

Color figures can only be printed if the author is prepared to pay for the extra cost incurred. At the

time of submission the author is asked to state whether he/she is willing to cover these expenses.

The prices (incl. VAT) are EUR 595 for one Figure, 990 for two, 1485 for three and 1980 for four. Click

hereto download the color figure order form.
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314/homepage/2446_color_fig.pdfhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314/homepage/2446_color_fig.pdfhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314/homepage/2446_color_fig.pdfhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314/homepage/2446_color_fig.pdfhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314/homepage/2446_color_fig.pdfhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314/homepage/2446_color_fig.pdf


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2.4.5. Guidelines for figure preparationFor initial submission, it is not necessary to submit graphics with high resolution; however, if your

manuscript is accepted, high resolution graphics will be required as detailed in this section. Each

figure should be given in a separate file. Please prepare your figures according to the following

guidelines: Crop, or scale, art to the size intended for publication; no enlargement or reduction should

be necessary. If this is not possible. Figures should be submitted in a format which can be

reduced to publication size i.e. a width of 5585mm or 120175mm.

Figure panels should be indicated by capital letters (A, B, C, etc.) on the top left hand corner

As far as possible, all comparable labels should have the same size lettering, and the font size

should be consistent throughout the Figures. Use standard fonts such as Times, Times New

Roman, Arial and Helvetica. Symbols and labels should be aheight of 1.52.0 mm at their

final published size.

Make sure that any labeling is legible against the background, and that lines are of a suitable

thickness. The recommended minimum line weight is 0.3 pt for black lines on a light

background, and 0.4 pt for white lines on a black background; do not define lines as hairlinewidth.

Gridlines are not allowed except for log plots.

Remove excess space and elements from around the image.

Type, lines or other elements not intended for publication should be removed before

submission.

Make the image orientation the same as that intended for publication.

Flatten images prior to submission, i.e. they should not contain layers and/or transparent

objects.

Figures should have the following resolution at their final published size:

Graphs 800-1200 DPIPhotos 400-800 DPI

Color (RGB) 300-400 DPI

Do not use higher resolutions than those given in the Table above as these will not improve

the quality of your image but will produce very large files.

An easy way to check the quality (resolution) of Figures files is to use the zoom function: if an

image viewed at 400% on screen is blurry (pixellated), then the image will not reproduce well

in print. An image viewed at 100% on screen may look fine but will not necessarily reproduce

well as the screen resolution is much lower (only 72-96 dpi) than that of a printing press.

Changing the size of a digital image changes the resolution; however, as the area is increased

the overall dpi actually remains the same; as an example, a photographic image that is threeinches (76.2 mm) across at final size should be saved as TIFF with a width of 900 pixels (final

size: 3 in; resolution: 300 dpi). If the size is increased to six inches, a width of 1800 pixels

would be required to obtain a resolution of 300 dpi.

Images (photos) with lettering should be saved at a higher resolution (minimum 600 dpi)

than a photographic image alone (minimum 400 dpi) to avoid the text and line art appearing

jagged.

Photographic images often produce very large files; however, most software has an option to

compress the file using LZW compression and this will produce smaller files, especially when

the image contains large areas of single color or repeating textures and patterns.

Keeping an image simple is the best way to produce good output. Try to avoid adding more

to your graph or illustration than is necessary. Avoid 3D charts, excessive shading, stipples,


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lines and symbols (if there are several symbols, try and add them to the legend rather than a

key).When using shades of grey or other tints, be wary of using shades too close together

an ideal separation is 20%.

Remember that supplying high-quality electronic artwork can reduce delays in production

time as it minimizes the need for artwork to be resupplied.

Image manipulation

Manipulation of images is unacceptable. All figures must accurately reflect the original data.

Information should not be enhanced, eliminated, added, obscured or moved. In cases where

manipulation is unavoidable, this should be clearly detailed in the Figure legend. All instruments,

software and processes used to obtain the images must be fully detailed in the manuscript either in

the Figure legends or in the Materials and methods section. Acceptable image manipulation includes

uniformly adjusting the contrast of an entire image and any control images, ensuring that all original

data, including the background, remains visible and that no new features are introduced. Cropping of

gels, or re-positioning of lanes/fields, is permitted providing that all alterations are clearly indicatedby the use of dividing lines in the image itself, vital data are not removed, an explanation of the

alterations is included in the Figure legend and images of full blots or gels that the figures are derived

from are supplied in the supporting information. Unacceptable manipulation includes, but is not

limited to, the enhancement of one feature/ band over others, removal of background noise/bands,

etc. Authors must be able to produce all data in their raw format upon editorial request.

2.4.6. Other guidelinesStatistical analysis standard requirements

Full statistical analysis of your data should be detailed in the manuscript, preferably in the Figurelegends to allow each Figure to be easily understood without reference to the main text of the

article. The name of each statistical test used and the results obtained (including a description of the

comparisons made to obtain the p values, if not clearly indicated in the Figures themselves) should

be stated. The number of samples, experiments, replicates etc. should be detailed; data presentation

(mean +/ SD or SEM etc.) should be noted. Authors are encouraged to analyze data across multiple

experiments, if at all possible, and should note that analyzing replicates within a single experiment

does not provide information regarding experimental reproducibility.

Structural formulae

Structural formulae should be drawn in the manuscript in the position where they belong. They mustbe numbered in consecutive order with the other figures.

Equations

Mathematical and chemical equations are to be written in the manuscript at the place in which they

belong and should be marked by Arabic numerals in parentheses in the right margin in the order of

their appearance.


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2.4.7. Supporting InformationSupporting Information can be a useful way for an author to include important but ancillary

information with the online version of an article. Examples of Supporting Information include

additional tables, data sets, figures, movie files, audio clips, 3D structures, and other related

nonessential multimedia files. Supporting Information should be cited within the article text, and adescriptive legend should be included. It is published as supplied by the author, and a proof is not

made available prior to publication; for these reasons, authors should provide any Supporting

Information in the desired final format. Where possible, authors are encouraged to submit

Supporting Information as a single PDF file.

For further information on recommended file types and requirements for submission, please visit:

http://authorservices.wiley.com/bauthor/suppinfo.asp

It is at the Editor-in-Chiefs discretion to decide which part of the manuscript will be published as

Supporting Information.

3. Online submission of manuscripts3.1. Original manuscriptsBiotechnology journaloffers a web-based manuscript submission and peer review system accessible

at: http://mc.manuscriptcentral.com/btj

To submit your manuscript online, please proceed along the following steps:

Prepare your manuscript and illustrations in the appropriate format, according to the

instructions given (see Section Format and style of manuscripts) and ensure that data are

given in the correct order and style for the journal.

Main text (including front material) as well as figure legends and tables (in this order) should

be given in one file, preferably saved in .doc or .rtf format. Figures should be submitted

separately (not included in the main document) in TIFF, EPS, PPT or the original format.

If you have not already done so, create an account for yourself in the system by clicking on

the Create an Account button.

The system will guide you through the submission process. Online help is available to you at

all times during the process. You are also able to exit/re-enter at any stage before finallysubmitting your work. All submissions are kept strictly confidential. To monitor the

progress of your manuscript throughout the review process, just login periodically and check

your Author Center.

All submissions will be converted to PDF format during the upload process. The system

automatically generates one PDF file which contains all parts of the manuscript.

Check the final PDF! The information you see will be the same as that seen by the editors

and reviewers, it is therefore critical that you ensure all information is provided in this file

prior to clicking the submit button.

If you have any questions concerning the online submission program, do not hesitate to contact the

Editorial Office [email protected].
http://authorservices.wiley.com/bauthor/suppinfo.aspmailto:[email protected]:[email protected]:[email protected]:[email protected]://authorservices.wiley.com/bauthor/suppinfo.asp


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3.2. Revised manuscriptsThe revised manuscript, as a file, on which all alterations are clearly marked and visible, should be

submitted via the submission site. The revised manuscript must be accompanied by a point-by-pointletter summarizing the changes that have been made in response to the referees comments. This

letter must be uploaded with the manuscript files as Supporting information for the Editor and Peer

review, which should appear as the first file in the final PDF, which is sent to the reviewers. Please

note that when revised manuscripts are submitted online, only the changed files need to be

replaced.

4. Manuscript publication4.1. Author ServicesOnce an article is accepted, Wileys Author Services provides the functionality for authors to track

the production progress of their articles. Authors will also receive free access to the final published

version of their articles.

When an article is accepted, the corresponding author receives an e-mail with a unique code and

link. Logging in to Author Services with the same e-mail address where the alert is received

automatically connects the author to the article. Alternatively, authors may register with any e-mail

address: use the "add article" feature and enter the unique code contained in the initial e-mail to

connect to the article. There may be a short delay from when the article is accepted by the journal

and when it has been received at Wiley-Blackwell.

Go tohttp://authorservices.wiley.com/bauthor/ for further information.

4.2. Proofs and reprintsGalley proofs will be sent as low-resolution PDF-file by e-mail together with a reprint order form. The

proofs should be carefully corrected following the instructions provided with the galley proofs. The

proofs together with the reprint order form should be completed and returned by e-mail to the

Editorial Office ([email protected]) within 48 h. Reprints ordered later in the production process are

more expensive. Authors may be charged for extensive alterations of their article that are a result ofwrong files supplied by the authors. Reprints and/or high resolution PDF can be ordered at prices

shown on the reprint order form. Note that authors will be provided a low resolution final PDF via

Wileys Author Services. Reprints always have the BTJ cover page of the issue in which the article is

printed.
http://authorservices.wiley.com/bauthor/http://authorservices.wiley.com/bauthor/mailto:[email protected]:[email protected]://authorservices.wiley.com/bauthor/http://authorservices.wiley.com/bauthor/http://authorservices.wiley.com/bauthor/mailto:[email protected]://authorservices.wiley.com/bauthor/


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4.3. Copyright transfer agreement / OnlineOpenThe need to sign the copyright transfer agreement depends on whether you would like to publish

your article Open Access:

4.3.1. Open Access: for authors choosing OnlineOpenIf the Open Access / OnlineOpen option is selected the corresponding author will sign the Open

Access Agreement (www.wiley.com/go/ctalsweinheimglobaloaa) which offers a choice of the:

Creative Commons Attribution License;

Creative Commons Attribution Non-Commercial License; or,

Creative Commons Attribution Non-Commercial -NoDerivs License

To preview the terms and conditions of these open access agreements please visit the Copyright

FAQs hosted on Wiley Author Services

http://authorservices.wiley.com/bauthor/faqs_copyright.aspand visit

http://www.wileyopenaccess.com/details/content/12f25db4c87/Copyright--License.html

If you select the OnlineOpen option and your research is funded by The Wellcome Trust or members

of the Research Councils UK (RCUK) you will be given the opportunity to publish your article under a

CC-BY license supporting you in complying with Wellcome Trust and Research Councils UK

requirements. For more information on this policy and the Journals compliant self-archiving policy

please visit:http://www.wiley.com/go/funderstatement.

4.3.2. No Open Access: authors sign the copyright transfer agreementIf the OnlineOpen option is not selected, the corresponding author will sign the copyright transfer

agreement (www.wiley.com/go/ctalsweinheimglobal). The terms and conditions of the copyright

transfer agreement can be previewed in the samples associated with the Copyright FAQs:

http://authorservices.wiley.com/bauthor/faqs_copyright.asp

4.4. Funder arrangementsCertain funders, including the NIH, Howard Hughes Medical Institute, members of the Research

Councils UK (RCUK) and Wellcome Trust require deposit of the Accepted Version in a repository after

an embargo period. Details of funding arrangements are set out at the following website:

http://www.wiley.com/go/funderstatement

4.4.1. InstitutionsWiley has arrangements with certain academic institutions to permit the deposit of the Accepted

Version in the institutional repository after an embargo period. Details of such arrangements are set

out at the following website:http://www.wiley.com/go/funderstatement
http://www.wiley.com/go/ctalsweinheimglobaloaahttp://www.wiley.com/go/ctalsweinheimglobaloaahttp://www.wiley.com/go/ctalsweinheimglobaloaahttp://authorservices.wiley.com/bauthor/faqs_copyright.asphttp://authorservices.wiley.com/bauthor/faqs_copyright.asphttp://www.wileyopenaccess.com/details/content/12f25db4c87/Copyright--License.htmlhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/ctalsweinheimglobalhttp://www.wiley.com/go/ctalsweinheimglobalhttp://www.wiley.com/go/ctalsweinheimglobalhttp://authorservices.wiley.com/bauthor/faqs_copyright.asphttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://www.wiley.com/go/funderstatementhttp://authorservices.wiley.com/bauthor/faqs_copyright.asphttp://www.wiley.com/go/ctalsweinheimglobalhttp://www.wiley.com/go/funderstatementhttp://www.wileyopenaccess.com/details/content/12f25db4c87/Copyright--License.htmlhttp://authorservices.wiley.com/bauthor/faqs_copyright.asphttp://www.wiley.com/go/ctalsweinheimglobaloaa


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Appendix A: BTJ Keywords

BTJ primary keywords/areas of expertise: select at least 1

Analytical biotechnology Biocatalysis

Biochemical engineering Biomaterials

Bioprocess engineering Downstream processing

Food biotechnology Computational biology

Green/plant biotechnology Metabolic engineering

Nanobiotechnology Omics

Red/medical biotechnology Science communication

Systems biology Synthetic biology

White/industrial biotechnology Other

BTJ secondary keywords/areas of expertise: select 3-4

Algae Antibodies Bacteria Biochemistry

Bioelectronics Bioenergy Bioengineering Bioethics

Biofuels Bioinformatics Biomaterials Biomedical engineering

Biophysics Biopolymers Bioreactor Biorefinery

Bioremediation Biosafety Biosensors Bioseparation

Biosynthesis Biotransformation Blue/marine

biotechnology

Cancer

Cell biology Cell culture Cellular engineering Cellular therapy

Chromatography Crystallization Diagnostics Diseases

Drug delivery Environmental

biotechnology

Enzymes Expression systems

Fermentation Food Fungi Gene expression

Gene regulatory network Gene therapy Genetic engineering Genetics

Genomics Glycobiotechnology High-throughput Imaging

Insect cells Mammalian cells Mass spectrometry Materials

Medical applications Metabolic flux analysis Metabolic network Metabolomics

Micro/nanofluidics Microarrays Microbiology Microreactors

Modeling Molecular biology Molecular engineering Plants

Polymers Process development Process monitoring Protein engineering

Protein expression Protein folding Protein purification Protein stability

Proteomics Recombinant proteins Regenerative medicine RNA interference

Scale-up/down Screening Signaling network Stem cellsTextile biotechnology Tissue engineering Transcriptomics Transgenic organisms

Translational medicine Vaccines Viruses Yeast


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Appendix B: Checklist for Authors

CHECKLISTWhich manuscript type?

1. Research Article: Describing complete

investigations. Maximum of 6500 words,including references, figure legends and tables.

Not more than 5 figures.

2. Review:Only invited by the Editors.

- Full-length Review: 8000 words maximum

- Mini-Review: 4000 words maximum

3. Technical Report:Short description ofthe

developmentof a novel method or an

improvement or noteworthy modification of an

already existing technique or platform used in

biotechnology. Maximum 4000 words, 3 display

elements (Figures and/or Tables)

4. Rapid Communication: Brief, timely and/or of

such importance that rapid publication is

warranted. Maximum 2500 words, 3 displayelements (Figures and/or Tables)

5. Other contribution:Forum column (500 to 2000

words), letter, meeting report, book review

Manuscript style and format OK?

Manuscripts should be submitted in English (American

spelling).

The first page of the manuscript should contain:

1. Title (concise, only standard abbreviations).2. Full author names (including first name) and the

name of their institute(s), using superscript

numbers after name to indicate multiple

affiliations.

3. Keywords (3 to 5).4. Correspondence: name, title, full postal address

and e-mail address of the author to whom all

correspondence (including galley proofs) is to be

sent.

5. Abbreviations: alphabetical order.The second page: abstract (maximum 200 words, only

standard abbreviations allowed)

Number the sections:

1 Introduction

2 Materials and methods

3 Results (or Results and discussion)

4 Discussion (or Concluding remarks)

5 References

Reference format OK?

References, including those in tables and figure

legends, should be numbered sequentially in square

brackets [2, 18] in the order in which they appear in

the text.

Reference list (collected at the end of the manuscript)

as in the following examples:

Journals:

[1] Liebeton, K., Eck, J., Identification and Expression in

E. coli of novel nitrile hydratases from the

metagenome. Eng. Life Sci. 2004, 4, 557562.

[2] Hugo, G., Schmoekel, F. E., Weber, J. C., Jason C., et

al., Bone repair with a form of BMP-2 engineered for

incorporation into fibrin cell ingrowth matrices.

Biotechnol. Bioeng. 2005, in press.

DOI:10.1002/bit.20168.

Books:

[3] Geis, A., Perspectives of genetic engineering of

bacteria used in food fermentations, in: Heller, K.J.

(Ed.), Genetically Engineered FoodMethods and

Detection, Wiley-VCH Verlag, Weinheim 2003, pp.

100118.

[4] Chrisou, P., Klee, H. (Ed.), Handbook of Plant

Biotechnology, John Wiley & Sons, 2004.

Note that

When more than 5 authors: list 4, et al. Papers in press: cite DOI.

Other serial publications: cite in the same style asjournals

Website addresses not as references but insertedin the text directly after the data to which they

refer

Abstracts and posters in meeting books must notbe cited unless they are generally accessible

Personal communication or unpublished datashould be cited in the text.

Figures and tables OK?

Tables with title and footnotes included at theend of the text

Figure legends at the end of the text Figures should be submitted in original format

(preferably TIFF, EPS or PPT) at high resolution

(300 dpi)

Please note that a fee has to be charged forcolor figure printing

Supporting information online

Extensive tables Text exceeding the maximum word count Other files like animations and video sequencesFigures and tables have to be accompanied by

appropriate legends. Has to be submitted along with

the manuscript body and in final form, preferably as

PDF. (no typesetting or galley proofs beforepublication)Cover letter complete?

Please state:

1. Why is the paper suitable for publication in BTJ?2. Do all the authors concur with the submission?3. Can you confirm that the work has not been

submitted to another journal and will not be

published elsewhere within one year after its

publication in this journal?

4. Does the manuscript contain experiments usinganimals?

5. Does the manuscript contain human studies?

biotecnology journal

Documents