BIO-ON srlvia dante 7/b

San Giorgio di Piano – 40016, Bologna - Italyweb: www.bio-on.itmail: info@bio-on.it

phone: +39051893001Primary contact: Marco Astorri, CEO

Bio-on’s strength is the ability to produce highly pure PHAs at a competitive price with comparable mechanical features to PP, PE, PET, HDPE, LDPE.

PHA Polyhydroxyalkanoate is a 100% natural polymer of the polyester family. To this day, it is considered to be the best polymer in the world.

TARGETED PROBLEM

Bio-on plastic is 100% biodegradable in bacteriological impure water in few days

Plastic pollution is "the accumulation in the environment of man-made plastic products to the point where they create problems for wildlife and

their habitats as well as for human populations”

• Today plastics and synthetic polymers are produced mainly from hydrocarbons (oil)

Bio-on can obtain plastic from the production of PHA: a truly biological plastic, result natural bacterial fermentation of agricultural wastes

• Today plastic recycle processes use large quantities of energy and chemical products

TO SOLVE THE PROBLEM• Bio-on’s aim is to work in the field of the modern biotechnologies applied to widely

used materials. Bio-on intention is to create 100% natural products/solutions based on renewable resources or agricultural processing waste materials;

• Bio-on offers a polymer technology: PHAs-Polyhydroxyalkanoates, a truly biological plastic for the full range of strategic applications. PHAs can compete with traditional plastics, PP, PE, PET, HDPE, LDPE;

• TARGET: Bio-on directly targets the following sectors: food-processing (production and distribution), medical and pharma pac, electronics, automotive, design and clothing and others. It provides all players with the technology required to produce and use PHAs in line with their own characterizations;

• Bio-on’ PHAs production:– uses agricultural by-products– does not use any raw material which is considered food (It does not use cereals)– does not use large quantities of energy nor chemical products for the production of biomaterials– does not use any of the traditional chemical products used for biopolymer recovery – modular production unit, easy to connect to existing plants and easy to expand anywhere– “Zero-miles” philosophy for the use of raw materials– No genetically modified plants or bacteria are used (OGM Free)

• Bio-on PHAs is 100% biodegradable both in soil and water, fresh and sea water: non forced biodegradability!

MARKET

• Bio-on issues licences enabling the production of MINERV-PHA™ to produce:

• April 2012 : Bio-on and FLOS present the first product in the world made entirely of PHAs bio plastic, naturally 100% biodegradable in water. To test this revolutionary material Bio-on has chosen MISS SISSI, the lamp PHILIPPE STARK designed for FLOS in 1991.

• March 2012: Bio-on has chosen to work with Techint Engineering & Construction to build plants for the production of PHAs bioplastic. The technology developed over the last five years by bio-on can now be created worldwide on an industrial scale, with plants producing 10 thousand tons/year.

• The plants currently producing PHA in the Italian head-quarter of Bio-On are on laboratory scale (6 liters fermentors) and pre-industrial scale (1.400 liters fermentors); the availability of these facilities allows the fine tuning of the conditions for fermentation, to direct the production of PHAs with different molecular structures.

TECHNOLOGY

A huge work of selection of natural bacteria along the years, avoiding any use of GMOs, allowed the development of several grades of PHAs, that are spontaneously accumulated as energy storage by bacteria feeded with sugars. Polyhydroxyalkanoate PHAs are 100% bio-based, fully biodegradable plastic materials that can be considered as the best candidates for traditional oil-based plastics replacement thanks to their excellent balance of physical, thermal and mechanical properties. Bio-on produces on its own and licences world-wide the production of PHA through its patented technology.

• From the selection of the bacterial strains isolated by Bio-on to the fermentation of beet sub products waste comes PHAs (Polyhydroxyalkanoate);

• The “white discs” represent the organic polymer minerv PHAs obtained from sugar beet. These elements are the result of increased bacteria nourished by the juices of beet (waste molasses): natural bacterial fermentation in a few dozen hours;

• The next step is the process of ‘recovery’: PHAs are retrieved and separated from the rest of the organic material, filtered and dried without any solvents;

• All waste material (in small quantity) is re-entered in the initial production cycle in order to feed new bacterial colonies together with the beet intermediate juices;

• From the PHAs powder extracted from bacteria we obtain PHA pellet for either injection or extrusion.

MARKETING STRATEGY• Scale up industrial processes by defining production modules. We create complete Engineering from the laboratory

to the production plant on an industrial scale, with production modules from 5 to 10 thousand tons per year.• Bio-on offers production technology licensing with design and implementation by qualified industry partners.• Bio-on has developed a partnership with the architect Enrico Iascone.• The low environmental impact of PHAs produced by Bio-on have encouraged him to design an eco-friendly

production module in the most sustainable way possible. It is a large, yet reasonably priced project that makes the optimal use of space for people, materials used and environmental impact. The modular structure is expandable and can be built anywhere in the world.

• The design incorporates an expandable PHA production capacity of 10 thousand tons per year.

Bio-on 1st Industrial PHAs production plant (2013) from sugar beet waste: Production, Labs, Office, Handling

NEXT STEP - 2013: Industry

COMPETITORS

Agricultural “waste” Material Food Material

GMO free

NO chemical solvent

Low energy use

IP & Engineering companyManufactory units

Low production costsHigh production costs

Heavy energy use

Heavy chemical solvent use

GMO

High logistics costs

Slow time to market

GMO Ww territory problem

NO licensing

Assuming a standard end user price per Kg. using the exclusive method Bio-on to extract the polymer have costs equal to 7%, while with the methods known today, which use large amounts of traditional chemistry, the cost is 70%.

Fast time to market

New Best bio polymers standard

PHA COMPETITORS

• Mr Marco Astorri, funded Bio-on in 2007 and at present he is President of the Company. He is the key administrative manager of Bio-On, coordinator of all R&D activities and in charge of defining the company strategies for industrial development.

• Mr Guido Cicognani, co-funded Bio-on in 2007 and at present he is the vice President of the Company. He collaborates with Astorri in the administrative, legal and financial tasks, he is in charge of innovation activities and knowledge exploitation.

• Dr Simone Begotti, Bio-on's head biologist, manages the laboratory research tasks needed to the develop several grades of PHAs. Dr Begotti is an experienced biotechnologist with wide skills in the field of biofermentation and biorefinery; in Bio-on labs he is supported by 4 technical chemist-assistants and strictly collaborates with fully equipped external research centers for the characterization of the PHA collected by the fermentors.

• Dr Luca Giamperi, Bio-on's chief materials development. His expertise in the field of polymer processing supports the team in the identification of the best chemical structures candidates for satisfying specific needs.

• 10 exclusive advisors experienced in different R&D themes and marketing strategy are involved in the Bio-on project assuring the quality of the products, their impact in the near future and the efficiency of the marketing and sales strategy.

TEAM

• Bio-on is an autonomous research entity funded in the form of small enterprise; figures in 2011:

– Employees: 6;– Turnover: €. 1.154.618,33 – Balance sheet: €. 104.113

• Bio-on’s richness is the know-how regarding the production of PHAs.• Bio-on has never raised money from banks, friends, family or angels. The company is

self-financed and almost all profit is annualy re-invested in Bio-on’s staff, labs and facilities.

• Bio-On would like to scale up industrial processes by realising industrial plants for the production of PHAs, from 5 to 10.000 tons/year, for the full range of strategic applications

• The expected/estimates revenue are:– In three years: redouble the actual one and reach around €. 2.300.000;– In five years: triplicate the actual one and reach around €. 3.500.000

• The capital needed for the next step is difficult to estimate and is linked to different factors: the marketing strategy is not fully yet decided (JV, investors, alliance, new partnership). The estimations on number of costumers and the conversion rate will follow it.

FINANCIALS

• Bio-on plastics are plastics in which all carbon is replaced by 100% renewable feedstocks.

• Bio-on plastics are 100% biodegradable both in soil and water, fresh and sea water in few days.

• To produce its plastics Bio-on:– uses agricultural “waste” material (no GMO, no food)– not use chemical solvents neither in the production nor in the extraction of PHAs– uses low energy– “zero-miles” philosophy in the whole chain– contributes to healthier rural economies

• Minerv PHAs (Bio-on plastic) is certified with the prestigious acknowledgment of its total and natural biodegradability in water and soil issued by AIB-VINCOTTE International. Bio-on is the first company in the world producing PHAs to get this important Certification year 2008.

• Bio-on has designed a low-cost project for the production of PHAs as sustainable and environmental-friendly as possible that increases the value of space for people and reduce the environmental impact: the modular structure is expandable and can be built all over the world.

SUSTAINABILITY

• 2012/2013: Bio-on is finalising the tests on its pilot plan and is mature enough to begin the 1st industrial plant for PHAs:

– Dimension: 10.000 mq.– Production: 10.000 tons/year PHAs from sugar beet waste.– Eco Building: no pollution.– New Modular concept: “The same PHAs Building around

the World”.

CURRENT STATUS• April, 2012: MISS SISSI designed by Philippe Stark and

produced with minerv PHAs is presentated world wide in Milan.

• The $50,000 First Prize would be invested in the Bio-on pilot plan to co-fund the purchase of a new dryer in order to conduct new research activities on PHA characterization.