atp and mitochondria

Click here to load reader

Post on 16-Jul-2015




0 download

Embed Size (px)


  • !!"






  • !" ##

    !$% !$! $$ $#$&

  • '

  • Vitamin SupplementationSome authors have recommended the following:25-150mg of the "basic Bs" - B1, B2, B3, B5, B6; 300-10,000mcg biotin100-1,000mcg B12400-2,000mcg folic acidMagnesium 400-800mg dailyAlpha Lipoic AcidCarnitineNADH

  • &!""""""")*+&





  • Goldstein S, Moerman EJ, Porter K. High-voltage electron microscopy of humandiploid fibroblasts during ageing in vitro. Morpho-metric analysis of mitochondria.Exp Cell Res. 1984;154:101111

    Brantova O, Tesarova M, Hansikova H, et al. Ultrastructural changesof mitochondriain the cultivated skin fibroblasts of patients with point mutations in mitochondrialDNA. Ultrastruct Pathol 2006;30:239245.

    Feldman D, Bryce GF, Shapiro SS. Mitochondrial inclusions in keratinocytes ofhairless mouse skin exposed to UVB radiation. J Cutan Pathol. 1990;17:96100.

    Guillery O, Malka F, Frachon P, et al. Modulation of mitochondrial morphology bybioenergetics defects in primary human fibroblasts. Neuromuscul Disord.2008;18:319330.

  • Lazarou M, McKenzie M, Ohtake A, Thorburn DR, Ryan MT.Analysis of the assembly profiles for mitochondrial and nuclear DNAencoded subunits into complex. Mol Cell Biol. 2007;27:42284237.

    Papa S. Mitochondrial oxidative phosphorylation changes in thelife span. Molecular aspects and physiopathological implications.Biochim Biophys Acta. 1996;1276:87105.

    Zwerschke W, Mazurek S, Stockl P, et al. Metabolic analysis ofsenescent human fibroblasts reveals a role for AMP in cellularsenescence. Biochem J. 2003;376:403411.

    Sugrue MM, Tatton WG. Mitochondrial membrane potential in agingcells. Biol Signals Recept. 2001;10:176188.McLennan HR, Degli Esposti M. The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species. J Bioenerg Biomembr. 2000;32:153162.

  • Crane FL. Biochemical functions of coenzyme Q10.J Am Coll Nutr. 2001;20:591598.

    Lopez-Lluch G, Barroso MP, Martin SF, et al. Role of plasma membranecoenzyme Q on the regulation of apoptosis. Biofactors. 1999;9:171177.

    Mellors A, Tappel AL. The inhibition of mitochondrial peroxidation by ubiquinone and ubiquinol. J Biol Chem. 1966; 241:43534356.

    Meyer LE, Machado LB, Santiago AP, et al. Mitochondrial creatinekinase activity prevents reactive oxygen species generation: Antioxidant role of mitochondrial kinase-dependent ADP re-cyclingactivity. J Biol Chem. 2006;281:3736137371.

    Lenz H, Schmidt M, Welge V, et al. Inhibition of cytosolic and mitochondrial creatine kinase by siRNA in HaCaT- and HeLaS3-cells affects cell viabilityand mitochondrial morphology. Mol Cell Biochem. 2007;306:153162.

  • Ponticos M, Lu QL, Morgan JE, et al. Dual regulation of the AMP-activatedprotein kinase provides a novel mechanism for the control of creatine kinase inskeletal muscle. EMBO J. 1998;17:16881699.

    Hornig-Do HT, von Kleist-Retzow JC, Lanz K, et al. Human epidermalkeratinocytes accumulate superoxide due to low activity of Mn-SOD,leading to mitochondrial functional impairment. J Invest Dermatol. 2007;127:10841093.

    Dzeja PP, Terzic A. Phosphotransfer networks and cellular energetics.J Exp Biol. 2003;206:20392047.

    Bessman SP, Carpenter CL. The creatine-creatine phosphate energy shuttle. Annu Rev Biochem. 1985;54:831862.

    Blatt T, Lenz H, Koop U, et al. Stimulation of skins energy metabolismprovides multiple benefits for mature human skin.Biofactors. 2005;25:179-185.

  • Birch-Machin MA. The role of mitochondria in ageing and carcinogenesis.Clin Exp Dermatol. 2006;31:548552.

    Jendrach M, Pohl S, Voth M, et al. Morpho-dynamic changes of mitochondria during ageing of human endothelial cells.Mech Ageing Dev. 2005;126:813821.Hansford RG. Bioenergetics in aging. Biochim Biophys Acta. 1983;726:4180.

    Frei B, Kim MC, Ames BN. Ubiquinol-10 is an effective lipid-soluble antioxidant at physiological concentrations. Proc Natl Acad Sci USA. 1990;87:48794883.

    Lass A, Kwong L, Sohal RS. Mitochondrial coenzyme Q content and aging. Biofactors. 1999;9:199205.

    Hoppe U, Bergemann J, Diembeck W, et al. Coenzyme Q10, a cutaneous antioxidant and energizer. Biofactors. 1999;9:371378.

  • Crayhon, R. The Carnitine Miracle. NYC: M. Evans, 1998

    Leibovitz, B. & Mueller, J. (1993) Carnitine J Opt Nutr 2: 90-119.

    Siliprandi, N. et al (1990) Metabolic changes induced by maximal exercise in human subjects following l-carnitine administration Biochim Biophys Acta 1034: 17-21.

    Birkmayer, G. NADH The Energizing Coenzyme. New Canaan: Keats. 1998.

    Berneburg M, Gremmel T, Kurten V, et al. Creatine supplementation normalizes mutagenesis of mitochondrial DNA as well as functional consequences. J Invest Dermatol. 2005;125:213220.

    Wolber R, Stab F, Max H, et al. Alpha-glucosylrutin, a highly effective flavonoid for protection against oxidative stress. J Dtsch Dermatol Ges. 2004;2:580587.

  • Lu CY, Lee HC, Fahn HJ, Wei YH. Oxidative damage elicited byimbalance of free radical scavenging enzymes is associated withlarge-scale mtDNA deletions in aging human skin.Mutat Res. 1999;423:1121.

    . Berneburg M, Grether-Beck S, Kurten V, et al. Singlet oxygenmediates the UVA-induced generation of the photoaging-associated mitochondrial common deletion. J Biol Chem.1999;274:153451534

    Hipkiss AR. Does chronic glycolysis accelerate aging? Could thisexplain how dietary restriction works?Ann N Y Acad Sci. 2006; 1067:361368

    Alikhani Z, Alikhani M, Boyd CM, et al. Advanced glycation endproducts enhance expression of pro-apoptotic genes and stimulatefibroblast apoptosis through cytoplasmic and mitochondrial path-ways. J Biol Chem. 2005;280:1208712095.

  • . Eshaghian A, Vleugels RA, Canter JA, et al. Mitochondrial DNAdeletions serve as biomarkers of aging in the skin, but are typically absentin nonmelanoma skin cancers. J Invest Dermatol. 2006;126:336344.

    Schroeder P, Gremmel T, Berneburg M, Krutmann J. Partialdepletion of mitochondrial DNA from human skin fibroblasts inducesa gene expression profile reminiscent of photoaged skin.J Invest Dermatol. 2008;128:22972303.

    Berneburg M, Plettenberg H, Medve-Konig K, et al. Induction of thephotoaging-associated mitochondrial common deletion in vivo innormal human skin. J Invest Dermatol. 2004;122:12771283.

    Yang JH, Lee HC, Wei YH. Photoageing-associated mitochondrialDNA length mutations in human skin.Arch Dermatol Res. 1995;287:641648.

  • Wei YH, Lu CY, Wei CY, Ma YS, Lee HC. Oxidative stress in humanAging and mitochondrial disease-consequences of defectivemitochondrial respiration and impaired antioxidant enzymesystem.Chin J Physiol. 2001;44:111.

    Cadenas E, Davies KJ. Mitochondrial free radical generation,oxidative stress, and aging. Free Radic Biol Med. 2000;29:222230.

    Droge W. Free radicals in the physiological control of cellfunction.Physiol Rev. 2002;82:4795.