anais congresso natal

Annals Of

2011 Symposium Organizing Committee

Presidents Dr. Arnóbio Antônio da Silva Júnior, UFRN/RN Dra. Maria das Graças Almeida, UFRN/RN Executive Committee Dra. Adriana Augusto de Rezende, UFRN/RN Dra. Aline Schwarz, UFRN/RN Dra. Ana Paula Barreto Gomes, UFRN/RN Dra. Ivanise Marina Moretti Rebecchi, UFRN/RN Dra. Janaína C. de Oliveira Crispim Freitas, UFRN/RN Dr. Guilherme Maranhão Chaves, UFRN/RN Dra. Lúcia Maria da Cunha Galvão, UFRN/RN Scientific Committee Dr. Anselmo Gomes de Oliveira, UNESP/SP Dr. Egler Chiari, UFMG/MG Dr. Eryvaldo Sócrates Tabosa do Egito, UFRN/RN Dr. Luiz Alberto Lira Soares, UFPE/PE Dr. Mario Hiroyuki Hirata, USP/SP Dr. Matheus de Freitas Fernandes Pedrosa, UFRN/RN Dra. Raquel Brandt Giordanni, UFRN/RN Dra. Silvana Zucolotto Langassner, UFRN/RN Dra. Vivian Nogueira Silbiger, UFRN/RN Scientific Evaluation Committee Dr. Bolívar P.G.L. Damasceno, UEPB-CG/PB Dr. Cícero Flávio Soares Aragão, UFRN/RN Dra. Cristiane C.G.O. Lopes, UFC/CE Dra. Francilene Amaral da Silva, UFS/SE Dr. Lívio César Cunha Nunes, UFPI/PI Dr. José Alexsandro da Silva, UEPB-CG/PB Dr. José Otávio Carréra Silva Júnior, UFPA/PA Dra. Júlia Beatriz Pereira de Souza, UFCG-CG/PB Dr. Najeh Maissar Khalil, UNICENTRO/PR Dra. Paula da Silva Kujbida, UFRN/RN Dr. Rand Randall Martins, UFCG-CG/PB Dr. Toshiyuki Nagashima Júnior, UFCG-CG/PB Dr. Geraldo Barroso Cavalcanti Júnior, UFRN/RN Dra. Antônia Claudia, UFRN/RN Dr.Túlio Moura, UFRN/RN Dr. Anselmo Oliveira Gomes, UNESP/SP Dr. Mário Hiroyuki Hirata, USP/SP Dr. Luiz Alberto Lira Soares, UFPE/PE Dr. Matheus de Freitas Fernandes Pedrosa, UFRN/RN Dra. Raquel Brandt Giordanni, UFRN/RN Dra. Silvana Zucolloto Langassner, UFRN/RN Dra. Vivian Nogueira Silbiger, UFRN/RN Dra. Julieta Genre, UFRN/RN Dra. Paula Machado, UFRN/RN Dra. Maria Joselice, UFRN/RN Dr. Euzébio Guimarães Barbosa, UFRN/RN

Student Organizing Committee Alessandro Brito dos Santos (Undergradute Pharmacy student-UFRN) Alice Rodrigues de Oliveira (graduate student-PPGCF-UFRN) Andréa Caroline Costa de Arruda (Undergradute Pharmacy student-UFRN) Clélio Diogo Soares (graduate student-PPGCF-UFRN) Cristhiano Sibaldo (graduate student-PPGCF-UFRN) Daliana Maria Berenice de Oliveira Souza (graduate student-PPGCF-UFRN) Elyson Diego Salustino Saravia Osorio (Undergradute Pharmacy student-UFRN) Emerson Michell da Silva Siqueira (Undergradute Pharmacy student-UFRN) Fabiana Paiva Galvão (graduate student-PPGCF-UFRN) Francisco Humberto Xavier Júnior (graduate student-PPGCF-UFRN) Gabriel Araujo da Silva (graduate student-PPGCF-UFRN) Heglayne Pereira Vital da Silva (graduate student-PPGCF-UFRN) Ipojucan Silva Fontes (Undergradute Pharmacy student-UFRN) Jahamunna Abrantes Andrade Barbosa (graduate student-PPGCF-UFRN) Janaina da Silva Goes (graduate student-PPGCF-UFRN) Julia Morais Fernandes (Undergradute Pharmacy student-UFRN) Juliana F. Carvalho (graduate student-PPGCF-UFRN) Juliana Félix Da Silva (Undergradute Pharmacy student-UFRN) Julieta Genre (graduate student-PPGCF-UFRN) Karla Samara Rocha Soares (graduate student-PPGCF-UFRN) Karla Simone Costa de Souza (graduate student-PPGCF-UFRN) Layany Carolyny da Silva Mourão (graduate student-PPGCF-UFRN) Leandro Vinicius Fernandes de Morais (Undergradute Pharmacy student-UFRN) Letícia Streck (graduate student-PPGCF-UFRN) Lidyane Neves Miranda (graduate student-PPGCF-UFRN) Margarete Moreno de Araujo (graduate student-PPGCF-UFRN) Monique Christine Salgado Gomes (graduate student-PPGCF-UFRN) Patrícia Fernandes Barbosa (Undergradute Pharmacy student-UFRN) Polyanne Nunes de Melo (graduate student-PPGCF-UFRN) Rafaela de Melo Pereira (graduate student-PPGCF-UFRN) Rayllan de Oliveira Rodrigues (Undergradute Pharmacy student-UFRN) Vitor Luiz de Brito Lemos (Undergradute Pharmacy student-UFRN) Waleska Rayane Dantas Bezerra de Medeiros (Undergradute Pharmacy student-UFRN) Walicyranison Plinio da Silva (graduate student-PPGCF-UFRN) Wenna Gleyce Araújo do Nascimento (graduate student-PPGCF-UFRN) Yamara Arruda Silva de Menezes (graduate student-PPGCF-UFRN) Yonara Monique da Costa Oliveira (graduate student-PPGCF-UFRN)

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PARTICIPANT AUTHOR TITLE PAGE

1 Paulo Ricardo Silva Pereira PEREIRA, PRS IMMUNOPHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF HEMATOPOIETIC STEM CELLS AFTER MOBILIZATION IN MALNOURISHED MICE

1

2 Arthur Ladeira Macedo MACEDO, AL IN VITRO ACARICIDAL AND CYTOTOXIC ACTIVITY OF AQUEOUS EXTRACTS OF AGAVE SISALANA PERRINE AND FURCRAEA SELLOA K. KOCH

2

3 Renata C. Vasconcelos VASCONCELOS, RMC PRELIMARY STUDY OF BORNEOL SALICILATE ANTIINFLAMATORY ACTIVITY 3

4 Mônica Oliveira Da Silva Simões

SIMOES MOS ESTIMATION OF CARDIOVASCULAR RISK BY THE FRAMINGHAM SCORE IN HYPERTENSIVE PATIENTS 4

5 André Leandro Silva SILVA, AL CHARACTERIZATION OF A CATIONIC NANOEMULSION INTENDED TO GENE THERAPY AFTER STERILIZATION PROCESSES

5

6 Celina Maria Pinto Guerra Dore CELINA M. P. G. DORE SV1: ONE POLYSACCHARIDE POPULATION FROM SARGASSUM VULGARE WITH ANTICOAGULANT AND ANTITHROMBOTIC ACTIONS AND THROMBIN INHIBITOR

6

7 Cíntia R. C. Porto PORTO, CRC ANTI-INFLAMMATORY AND ANTINOCICEPTIVE ACTIVITIES OF PHYLLANTHUS NIRURI L SPRAY-DRIED EXTRACT 7

8 Fernanda Rodrigues Salazar SALAZAR, FR DETERMINATION OF RALOXIFENE HYDROCHLORIDE USING ULTRAVIOLET SPECTROPHOTOMETRY AND LC STABILITY-INDICATING METHOD

8

10 Andressa Santa Brigida Da Silva

SILVA, ASB AQUEOUS EXTRACT OF MIKANIA LINDLEYANA DC. PRESENTS POTENTIAL ANTI-INFLAMMATORY EFFECT 9

12 Ana Carolina Kogawa KOGAWA, A.C. DEVELOPMENT AND VALIDATION OF DOXYCYCLINE HYCLATE BY HPLC METHOD 10

13 Anderson Angel Vieira Pinheiro PINHEIRO, A.A.V. STRATEGY FOR TREATMENT OF WATER, FEDERAL UNIVERSITY OF CAMPINA GRANDE / CENTER FOR EDUCATION AND HEALTH-PB

11

14 Áurea Marcela De Souza Pereira

PEREIRA, A.M.S. ADVERSE EVENTS IDENTIFIED IN A HOSPITAL OF THE SENTINEL NETWORK 12

15 Bárbara Coeli SILVA, B. C. O STANDARDIZATION OF TECHNIQUES FOR EVALUATION OF HEMATOPOIESIS IN MALNOURISHED MICE 13

16 Clarissa Nunes De Assis ASSIS, C. N. REFLECTIONS OF A CUSTODY CHAIN IN CENTER OF PHARMACEUTICAL EQUIVALENCE 14

17 Débora Freitas Do Nascimento NASCIMENTO, D.F. NANOCOSMETICS IN PHOTOPROTECTION: DEVELOPMENT AND CHARACTERIZATION OF NANOPARTICLES WITH SUNSCREENS

15

20 Margareth Linde Athayde ATHAYDE, M. L. ANTIMYCOBACTERIAL ACTIVITY OF THE FRACTIONS AND COMPOUNDS FROM SCUTIA BUXIFOLIA REISSEK 16

21.1 Monique Gabriela ALVES, M. G. C. F DIRECT INHIBITORY ACTION OF SULFATED POLYSACCHARIDES FROM RED SEAWEED HYPNEA MUSCIFORMIS ON THROMBIN ENZYMATIC ACTIVITY EVALUATED BY CHROMOGENIC SUBSTRATE ASSAY

17

21.2 Monique Gabriela ALVES. M. G. C. F INHIBITORY ACTION ON LIPID PEROXIDATION AND PROTECTIVE ACTIVITY ON H2O2-INDUCED HEMOLYSIS OF SULFATED POLYSACCHARIDES F1.0 FROM RED SEAWEED HYPNEA MUSCIFORMIS

18

23 Nially Karen Morais Do Amaral NENHUM AUTOR SUBLINHADO

DETERMINATION OF ACIDITY IN CURD CHEESE OF CAICÓ AND JUCURUTU 19

II INTERNATIONAL SYMPOSIUM ON PHARMACEUTICAL SCIENCES: Challenge and opportunities for development new diagnosis methods and pharmaceutical products

ABSTRACTS

24 Adalgiza D. V. Silva SILVA. A. D. V. TOXICOLOGIC ASSAY OF ETHANOLIC EXTRACT FROM XYLOPIA CAYENNENSIS 20

25 Everton Do Nascimento Alencar

ALENCAR. E. N ANTIMICROBIAL ACTIVITY EVALUATION OF COPAIBA OIL MICROEMULSION SYSTEM: A PRELIMINARY STUDY 21

26 Julius Roberto Martins De Oliveira

OLIVEIRA. J. R. M. DE PROFILE AND QUALITY OF MEDICATIONS PRESCRIPTION IN THE CITY OF CUITÉ – PB. BRAZIL 22

27.1 Mallone Lopes Da Silva NENHUM AUTOR SUBLINHADO

ANÁLISE FITOQUÍMICA E CROMATOGRÁFICA DE MIKANIA GLOMERATA 23

27.2 Mallone Lopes Da Silva NENHUM AUTOR SUBLINHADO

INVESTIGATION OF METABOLITES FROM XYLOPIA CAYANNENSIS 24

28 Rosilene Rodrigues Santiago SANTIAGO. R. R. ZINC OXIDE EMULSION: A NEW ALTERNATIVE TO DIAPER DERMATITIS TREATMENT 25

30 Almária Mariz Batista BATISTA. A. M. LEGAL ANALYSIS OF MEDICINES ADVERTISING TRANSMITTED IN RADIO STATION FROM NATAL/RN ACCORDING TO RDC N° 96/08

26

31.1 Rosalina Coelho Jácome JÁCOME. R. C STUDY OF ISONIAZID ADSORPTION ONTO CHITOSAN: ASSAYS OF EQUILIBRIUM AND KINETICS USING STIRRED TANK

27

31.2 Rosalina Coelho Jácome JÁCOME. R. C HOUSEHOLD SURVEY ABOUT THE PROFILE OF DRUG USE BY ELDERLY IN THE CITY OF CUITÉ-PB 28

32 Cristhiano Sibaldo ALMEIDA. C.S. ORAL ADMINISTRATION OF EXTRACT CYANOBACTERIAL BLOOM IN PREGNANT RATS: A REPRODUCTION ASSESSMENT

29

33 Daisy Dayane Formiga De Souza Lima

LIMA. D.D.F.S COMPARATIVE ANLYSIS OF PHYSICAL AND CHEMICAL PROPERTIES OF TWO SOURCES OF WATER FOR USE ON SCHOLL PHARMACY MANUEL CASADO ALMEIDA. CUITÉ-PB

30

34.1 Eliane Gandolpho Tótoli TÓTOLI. E. G. QUALITATIVE TESTS OF AMPICILLIN SODIUM POWDER FOR INJECTION 31

34.2 Eliane Gandolpho Tótoli TÓTOLI. E. G. DEVELOPMENT AND VALIDATION OF TURBIDIMETRIC ASSAY TO POTENCY EVALUATION OF AMPICILLIN SODIUM FOR INJECTION

32

35 Ivson Medeiros MEDEIROS JR. I. J. TECHNICAL AND ADMINISTRATIVE MANAGEMENT OF SCHOOL PHARMACY'S WAREHOUSE MANOEL CASADO DE ALMEIDA – UFCG/CES.

33

36 Jose Marcos Dos Santos Oliveira

OLIVEIRA. J. M. S. VALIDATION OF A SPECTROPHOTOMETRIC METHOD (UV-VIS) FOR DETERMINATION OF TOTAL FLAVONOIDS OF THE RED PROPOLIS FROM ALAGOAS: USE OF CATECHIN AS BIOMARKER

34

37.1 Keine Monteiro Castro CASTRO K.M. CONSTITUENTS STUDY OF FIXED PIKE NORTHEAST ( CARYOCAR CORIACEUM WITTMER.) 35

37.2 Keine Monteiro Castro CASTRO K.M. GUIDANCE ON PHARMACEUTICAL PREPARATIONS MEDICINAL PLANTS WITHHOMEMADE: A STRATEGY FOR FAMILY HEALTH

36

38 Larice Brito De Vasconcelos VASCONCELOS. L. B. STUDY OF THE TOXICITY OF ESSENTIAL OIL OF CYMBOPOGON CITRATUS (DC) STAF. FOR A POSSIBLE APPLICATION AS ANTIFUNGAL DRUG

37

39.1 Luis Fabio Dos Santos Gomes GOMES.L.F.S. PRELIMINARY STUDY OF THE TOXICITY OF ASPIDOSPERMA EXCELSUM BENTH (APOCYNACEAE). 38

39.2 Luis Fabio Dos Santos Gomes GOMES.L.F.S. ACUTE ORAL TOXICITY EVALUATION OF THE DRY ETHANOLIC EXTRACT OF HIMATANTHUS ARTICULATUS AND ELEUTHERINE PLICATA

39

40.1 Mayara Ladeira Coelho COÊLHO. M. L.

HISTOPATHOLOGICAL STUDIES OF THE EFFECTS OF THE AQUEOUS EXTRACT OF THE ORBIGNYA PHALERATA MART (BABASSU) MESOCARP POWDER IN ADULT MICE TREATED ORALLY

40

40.2 Mayara Ladeira Coelho COÊLHO. M. L.

THERMAL CHARACTERIZATION OF BABASSU (ORBIGNYA PHALERATA MART) MESOCARP POWDER COMPARED WITH THE RAW MATERIAL MICROCRYSTALLINE CELLULOSE

41

41 Severina Denise Sales De Oliveira

NENHUM AUTOR SUBLINHADO

STUDY AND CHARACTERIZATION SPECTROSCOPIC OF THE COMPLEX POTASSIUM HEXACYANOFERRATE 42

42 Tassia Tatianna Firmino FIRMINO. T.T.D. MEASUREMENT OF TOTAL FLAVONOIDS IN THE HYDROALCOHOLIC EXTRACTS OF RED PROPOLIS FROM ALAGOAS

43

43 Yohana Souza Pinto PINTO. Y.S. COMPARISON OF METHODS FOR DETERMINATION OF CONTENT PROTEIN IN THE AQUEOUS AND ACETONE EXTRACT ANNONA SQUAMOSA

44

44 Alaine Maria Dos Santos Silva SILVA. A.M.S PHYSICO-CHEMICAL PARAMETERS OF LANETTE® AND POLAWAX® EMULSIONS FOR “MANOEL CASADO DE ALMEIDA”/UFCG. PB PHARMACY SCHOOL PRODUCTION

45

45 Clarissa Nunes De Assis ASSIS. C. N MICROBIOLOGICAL ANALYSIS OF WATER FROM DIFFERENT SOURCES OF FORTALEZA IN THE PERIOD JANUARY TO SEPTEMBER 2011

46

46 Lisllie Dayse Araújo Santos SANTOS. L.D.A UTILIZATION OF MG. AL LAYERED DOUBLE HYDROXIDES (LDHS) AS A CARRIER OF DIGOXINE FOR VECTORIZATION IN CHEMOTERAPY OF CANCER

47

47 Livaldo Costa E Silva SILVA. LIVALDO COSTA E

CREATION OF DIGITAL'S MAGAZINES ON RATIONAL USE OF DRUGS: NEW PERSPECTIVES ON EDUCATION 48

48 Marana Silveira Ali ALI. M.S. CHRONIC. BUT NOT ACUTE. ETHANOL WITHDRAWL AFTER CHRONIC CONSUMPTION PROMOTES DEPRESSIVE-LIKE BEHAVIOUR WITHOUT ALTERING LOCOMOTION IN RATS

49

49.1 Rayanne Karen Cunha Gurgel GURGEL. R. K. C APPLICATION OF THERMOANALYTICAL METHODS IN EVALUATING THE BEHAVIOR OF TUBERCULOSTATICS DRUGS PRODUCED BY NUPLAM / UFRN: ISONIAZID AND PYRAZINAMIDE

50

49.2 Rayanne Karen Cunha Gurgel GURGEL. R. K. C DETERMINATION OF PHYSICAL AND CHEMICAL COMPOSITION FOR THE QUALITY CONTROL OF MILK CONSUMED IN THE CITY OF NATAL / RN

51

50 Lorena Citó Lopes Resende Santana

SANTANA. LCLR. STUDY OF PHYSICOCHEMICAL PROPERTIES AND ACUTE TOXICITY EVALUATION OF ETHANOLIC EXTRACT OF THE LEAVES FROM MIKANIA GLOMERATA SPRENGEL.

52

51 Nayara Maciel Mendonça MENDONÇA. N.M EPIDEMIOLOGY OF CANDIDEMIA IN PUBLIC HOSPITALS OF ALAGOAS. BRASIL 53

52.1 Andréia Cristina Wildner Campos Lopes

WILDNER A. C. VALIDATION OF ANALYTHICAL METHODOLOGY FOR PASSIFLORA INCARNATA L. EXTRACT BY HPLC AND ANALYISIS OF COMMERCIALS EXTRACTS FROM RS

54

52.2 Andréia Cristina Wildner Campos Lopes

MENEGHINI. LC DEVELOPMENT AND VALIDATION OF A METHOD OF DISSOLUTION FOR DARIFENACIN EXTENDED-RELEASE TABLETS BASED ON IN VIVO DATA

55

53.1 Luiza Sheyla Evenni Porfírio Will

WILL. L.S.E.P. LOBOPHORA VARIEGATA AND ITS ACTIVITY IN PAW EDEMA AND HEPATOTOXICITY IN RATS 56

53.2 Luiza Sheyla Evenni Porfírio Will

WILL. L.S.E.P. Β-GLUCANS OF CARIPIA MONTAGNEI MUSHROOM SHOWS ANTI-INFLAMMATORY ACTIVITY: NEWS PERSPECTIVE OF PPAR ATION

57

54 Maria Tatiane Dias Moura NENHUM AUTOR SUBLINHADO

EVALUATION OF HYPOTENSIVE RESPONSES OF LYOPHILIZED AYAHUASCA TEA IN SHR RATS 58

55.1 Marilia Da Silva Nascimento NASCIMENTO. M.S. ANGIOGENESIS INHIBITION AND B-GLUCANS ANTITUMOR ACTION OF CARIPIA MONTAGNEI MUSHROOM 59

55.2 Marilia Da Silva Nascimento NASCIMENTO. M.S. CARIPIA MONTAGNEI MUSHROOM B-GLUCANS SHOWING NON HEMOLYTIC ACTION AND ANTIOXIDANT ACTIVITY: POTENTIAL FUNCTION IN LIPIDIC PEROXIDATION INHIBIT AND OXIDATIVE STRESS

60

56 Luciane De Lira Teixeira NENHUM AUTOR SUBLINHADO

DETERMINATION OF MOISTURE AND LIPIDS IN FRUITS OF LICANIA TOMENTOSA BETH (OITI) 61

57 Prycila Rodrigues Feitoza NENHUM AUTOR SUBLINHADO

EVALUATION OF THE CARDIOVASCULAR EFFECTS OF SYZIGIUM CUMINI SEEDS 62

58.1 Thuane De Sousa Pinheiro PINHEIRO. T.S. POLYSACCHARIDES EFFECTS FROM LOBOPHORA VARIEGATA IN A TOPICAL INFLAMMATORY MODEL 63

58.2 Thuane De Sousa Pinheiro PINHEIRO. T.S. ANTIOXIDANT ASSAYS OF BRUTE EXTRACTED FROM PLECTTRANTUS BARBATUS ANDR. 64

59 T T SILVA JÚNIOR. J.O.C. EVALUATION OF DENTIN ROUGHNESS AFTER USE OF DIFFERENTS CHELATING AGENTS EMPLOYED IN ENDODONTICS

65

61 Mariana De Macedo Costa COSTA. M.M TOPICAL APPLICATION OF PROPOLIS FOR HEALING OF WOUNDS SURGICALLY INDUCED AND EVALUATION OF BIOCHEMICAL PARAMETERS IN WISTAR RATS

66

62 Márcia Natália Da Silveira Pinto PINTO. M. N. S EVALUATION CYTOTOXIC IN VITRO OF NEW COMPOUND LPSF/MS-02 67

63 Roberta Da Silva Campos CAMPOS. R.S. DETERMINATION OF PROTEIN CONTENT OF ZIZYPHUS JOAZEIRO 68

64 Thales Allyrio Araújo De Medeiros Fernandes

FERNANDES. T.A.A.M. COMPARISON OF LABORATORY CHARACTERISTICS OF PATIENTS WITH SICKLE CELL DISEASE 69

65.1 Tahisa Marcela Pedroso NENHUM AUTOR SUBLINHADO

UV SPECTROPHOTOMETRIC PROFILE OF CEFAZOLIN SODIUM 70

65.2 Tahisa Marcela Pedroso NENHUM AUTOR SUBLINHADO

A NEW. SAFE AND EASY METHOD OF THIN-LAYER CHROMATOGRAPHY FOR CEFAZOLIN SODIUM IDENTIFICATION

71

66 José Couras Silva Filho SILVA-FILHO. J. C. VOLTAGE-OPERATED POTASSIUM CHANNELS ARE INVOLVED IN (-)-BORNEOL-INDUCED VASORELAXANT RESPONSE ON RAT MESENTERIC ARTERY

72

67 Katyane Rodrigues RODRIGUES. K. PREPARATION OF NANOSTRUCTURED SYSTEMS SUNSCREENS ORGANIC – INORGANIC 73

68 Luíza Xavier XAVIER. L.A ANTIBACTERIAL EVALUATION OF NEW DERIVATIVES OF THIOPHENE 74

69 Camila Alves De Moura MOURA.C.A THEORETICAL AND PRACTICAL HANDBOOK: A TOOL FOR THE TRAINEESHIP LIVE PHARMACIES 75

70 Elyson Diego Salustino Saravia Osorio

OSORIO. E. D. S. S. DEVELOPMENT OF STRATEGIES OF MOLECULAR DIAGNOSTIC APPLIED TO DYSLIPIDEMIA 76

71 Flávia Danniele Frota Machado MACHADO. F.D.F

EVALUATION OF THE ROLE OF THE NITRIC OXIDE IN GASTROPROTECTIVE ACTIVITY OF PARTITION FRACTIONS OF NEOGLAZIOVIA VARIEGATA MEZ. (BROMELIACEAE) IN MODEL OF ULCERS INDUCED BY ETHANOL

77

72 Izadora De Souza DE SOUZA. I. OPTIMIZATION OF AN EXTRACTION PROCEDURE FOR THE BIOPOLYMER CHITIN 78

73.1 Danielle Cristina Zimmermann Franco

ZIMMERMANN-FRANCO. D. C.

USE OF POTENTIALLY INAPPROPRIATE MEDICATIONS AND THE RISK OF POSSIBLE DRUG INTERACTIONS BETWEEN INSTITUTIONALIZED FRAIL ELDERLY IN A CITY IN THE ZONA DA MATA MINEIRA

79

73.2 Danielle Cristina Zimmermann Franco

ZIMMERMANN-FRANCO. D. C.

ANTIFUNGAL ACTIVITY OF OLEORESIN EXTRACTED FROM COPAIFERA LANGSDORFFII DESF AGAINST DERMATOPHYTES FUNGI

80

74 Margarete Moreno De Araujo DE ARAUJO. M. M. EVALUATION OF ACCELERATED STABILITY OF SOYBEAN O/W EMULSION CONTAINING ETHANOL AND BENZYL ALCOHOL AS COSURFACTANTS

81

75 Mariana Araújo Paulo De Medeiros

MEDEIROS. M. A. P EVALUATION OF MORPHOGENESIS AND PROTEINASE ACTIVITY OF CLINICAL ISOLATES OF C. ALBICANS OBTAINED FROM PATIENTS WITH VULVOVAGINAL CANDIDIASIS

82

76 Cristiane Franco Codevilla CODEVILLA C.F. DETERMINATION OF LODENAFIL CARBONATE USING CAPILLARY ZONE ELECTROPHORESIS METHOD 83

77 Melissa Alexandra Da Rosa Costa

COSTA. M .A. R. ANALYSIS OF EPIDEMIOLOGICAL SITUATION OF LEPROSY IN CHILDREN UNDER 15 YEARS OLD IN FORTALEZA IN THE PERIOD OF 2001 TO 2010

84

78.1 Érika Yoko Suzuki SUZUKI. E.Y IN VITRO INHIBITION OF CLINICAL PSEUDOMONAS AERUGINOSA ADHESION BY XYLITOL 85

78.2 Érika Yoko Suzuki SUZUKI. E.Y ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL FROM BACCHARIS TRIMERA (LESS) DC 86

80 Daiane Soares SOARES. D.S PHYSICAL-CHEMICAL COMPATIBILITY BETWEEN ATTAPULGITE. RIFAMPICIN AND ISONIAZID 87

81 Elquio Eleamen Oliveira MARCELINO. H. R. NEW METHOD TO PRODUCE XYLAN MICROPARTICLES USING SODIUM TRIMETAPHOSPHATE AS CROSS-LINKING AGENT

88

82 Jéssica Guedes GUEDES. J. S. SYNTHESIS OF TIN (IV) COMPLEXES WITH POTENTIAL ANTIMICROBIAL ACTIVITY 89

83 Jôseffer Wianey Trindade Medeiros

MEDEIROS. J. W. T. ADVERSE DRUG REACTIONS AS A CAUSE OF HOSPITAL ADMISSION 90

84.1 Juliana Félix Da Silva FÉLIX-SILVA. J ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS LEAF EXTRACT OF JATROPHA GOSSYPIIFOLIA L. IN CARRAGEENIN-INDUCED PAW EDEMA IN RATS

91

84.2 Juliana Félix Da Silva FÉLIX-SILVA. J ANTI-INFLAMMATORY ACTIVITY OF BULLFROG (RANA CATESBEIANA) OIL IN CARRAGEENIN INDUCED PAW EDEMA IN RATS

92

86 Maria Luiza Gois Campos CAMPOS. M. L. G INCLUSION COMPLEXES OF FUROSEMIDE WITH Β-CYCLODEXTRIN AND PVP 93

87.1 Nathália Alexandra De Oliveira Cartaxo

CARTAXO. N.A.DE O. RATIONAL USE OF DRUGS IN HYPERTENSION: AN ACHIEVEMENT OF PHARMACEUTICAL CARE 94

87.2 Nathália Alexandra De Oliveira Cartaxo

CARTAXO. N.A.DE O. EXTRACTION OF FLAVONOIDS AND POLYPHENOLS IN LEAVES OF MENTHA X PIPERITA L. PROCESSED IN FIXED BED CONVECTIVE DRYER

95

90 Andressa Noronha Barbosa Da Silva

SILVA. A. N. B. DISTRIBUTION OF TRIATOMINES FAUNA AND NATURAL INFECTION BY TRYPANOSOMA CRUZI IN THE SEMIARID OF THE STATE OF RIO GRANDE DO NORTE. BRAZIL

96

91 Michelle De Oliveira Pedrosa PEDROSA. M.O. DEVELOPMENT OF THIOPHENES DERIVATIVES BIOCOMPATIBLES MICROEMULSIONS 97

92 Karolyne Dos Santos Silva SILVA. K. S INFLUENCE OF PLURONIC® F68 ON THE BENZATHINE PENICILLIN G ANTIBACTERIAL ACTIVITY 98

93 Máyra Furtado Dias Filgueira Thé

THÉ. M. F. D. F. EVALUATION OF THE ANTIFUNGAL ACTIVITY OF NOR-LAPACHONE DERIVATIVE AGAINST ISOLATES OF CANDIDA SPP.

99

94 Jacyra Antunes Dos Santos Gomes

GOMES. J. A. S. QUALITATIVE PHYTOCHEMICAL PROFILE OF JATROPHA GOSSYPIIFOLIA L. EXTRACTS PREPARED BY DIFFERENT METHODS OF EXTRACTION

100

95.1 Magda Rhayanny Assunção Ferreira

FERREIRA. M. R. A EVALUATION OF ANTIFUNGAL ACTIVITY OF CRUDE EXTRACT FROM CAESALPINIA FERREA MART AGAINST CLINICAL ISOLATES FROM THE ORAL CAVITY OF RENAL TRANSPLANTATION

101

95.2 Magda Rhayanny Assunção Ferreira

FERREIRA. M. R. A BIOASSAY-GUIDED PURIFICATION OF CRUDE EXTRACT FROM STEM BARKS FROM CAESALPINIA FERREA MART (PAU FERRO)

102

96 Walicyranison Plinio Da Silva SILVA. W.P IDENTIFICATION AND PHOSPHOLIPASE ACTIVITY OF FUNGI ISOLATED FROM DERMATOMYCOSES OF CHILDREN ATTENDING MUNICIPAL CENTERS OF CHILDREN EDUCATION FROM NATAL-RN

103

97 Patrícia Barbosa BARBOSA. P.F. EVALUATION OF THE REPRODUCTIVE AND FETAL TOXICITY OF CYLINDROSPERMOPSIN IN THE RAT 104

98.1 Maria Aparecida Alves Leite Dos Santos Almeida

ALMEIDA. M.A.A.L.S DEVELOPMENT OF A LIQUID DETERGENT FORMULATION FOR USE IN UFCG/CES AND EVALUATION OF VISCOSITY AND POWER FOAM

105

98.2 Maria Aparecida Alves Leite Dos Santos Almeida

ALMEIDA. M.A.A.L.S RETROSPECTIVE STUDY OF ADVERSE DRUG REACTIONS IN THE PEDIATRIC WARD OF THE UNIVERSITY HOSPITAL ALCIDES CARNEIRO. CAMPINA GRANDE / PB

106

99 Alexandre Medeiros Dantas OLIVEIRA. A. R. IN VITRO RELEASE OF METHOTREXATE-LOADEDSPRAY DRIED PLGA MICROPARTICLES 107

101 Letícia Streck STRECK. L. BENZNIDAZOLE-LOADED SOYBEAN O/W EMULSION SYSTEM 108

102 Milene Evelyn Da Silva NENHUM AUTOR SUBLINHADO

EVALUATION OF CITRONELLA (CYMBOPOGON WINTERIANUS JOWITT EX BOR.) OIL COMPOSITION EXTRACTED FRESH AND DRY PLANT AND INFLUENCE OF HARVESTING PERIOD

109

103 Cynthia Layse Ferreira De Almeida

ALMEIDA. C. L. F.

SCREENING FOR ANTIBACTERIAL ACTIVITY ANTIMICROBIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF ANADENANTHERA MACROCARPA BENTH AND MYRACODRUOM URUNDEUVA ALL. AGAINST STRAIN OF STAPHYLOCOCCUS AUREUS

110

104 Anne Kaliery De Abreu Alves ALVES. A.K.A. OUABAIN REDUCES THE PHAGOCYTOSIS OF PERITONEAL MACROPHAGES 111

105 Manuelle Prestrelo De Oliveira NENHUM AUTOR SUBLINHADO

EVALUATION OF THE RELAXING ACTIVITY OF ETHANOL EXTRATCT FROM LEAVES ASPIDOSPERMA MACROCARPUM IN ISOLATED RAT MESENTERIC ARTERY

112

106 Luis Paulo Da Penha Ferino FERINO. L.P.P. EVALUATION OF TESTS PRE-TRANSFUSIONAL IMMUNO-HEMATOLOGICAL IN RECEPTORS OF BLOOD 113

107 Ricardo Bizogne Souto SOUTO R. B. DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE LIQUID CHROMATOGRAPHY METHOD FOR THE EVALUATION OF RECOMBINANT HUMAN INTERLEUKIN-11. CORRELATION WITH THE BIOASSAY

114

108 Fernanda Pavani Stamm STAMM. F. P STUDY OF A RP-LC METHOD FOR THE ANALYSIS OF RECOMBINANT PARATHYROID HORMONE IN BIOPHARMACEUTICAL FORMULATIONS

115

109 Polyanne Nunes De Melo MELO. P. N. EFFECT OF TRIETHANOLAMINE/METHYL-1-PYRROLIDONE-2/BETACYCLODEXTRIN MULTICOMPONENT COMPLEXES SYSTEMS ON THE BENZNIDAZOLE APPARENT SOLUBILITY

116

111.1 Carolina Moraes Cabé CABÉ. C. M PHYSICAL PARAMETERS OF QUALITY ASSESSMENT OF DIFFERENT LOTS OF HOMEOPATHIC GLOBULES IN USE IN NATAL /RN/BRAZIL

117

111.2 Carolina Moraes Cabé CABÉ. C. M SPECTRAL ANALYSIS (FT-IR. UV) OF RELEASE OF LEACHABLES FROM NATURAL RUBBER BULBS 118

112 Horacinna Maria De Medeiros Cavalcante

CAVALCANTE. H. M. M. TOXICITY OF DENTAL VARNISH CHITOSAN 119

113 Edeildo Ferreira SILVA JÚNIOR. E. F. NEW TALENT PROGRAM – EDUCATION FOR HEALTH – ANALYSIS AND EDUCATION IN WATER USE IN THE OF TRAIPU – ALAGOAS

120

114 Katharina Rodrigues De Lima Porto Ramos

RAMOS. K. R. L. P. EVALUATION OF THE CARDIOVASULAR RISK IN CONNECTION TO C-REACTIVE PROTEIN 121

115 Antônio Vinícius Doriguetto Ferreira

FERREIRA. A. V. D CHEMICAL PROFILE VERIFICATION OF THE EXTRACTS AND SEMI-PURIFIED FRACTIONS OF ANDIRA NITIDA MART. EX BENTH BY TLC

122

116 Leonardo Augusto Rêgo De Souza


PRO-INFLAMMATORY AND CYTOTOXIC ACTION CARRAGEENAN OF THE SEAWEED AMANSIA MULTIFIDA 123

117.1 Geovani Pereira Guimarães GUIMARÃES. G. P ANTIFUNGAL ACTIVITY OF MEDICINAL PLANTS AGAINST CANDIDA SPECIES 124

117.2 Geovani Pereira Guimarães GUIMARÃES. G. P DEVELOPMENT OF A LSVM-5CN05 TRANSDERMIC MICROEMULSION 125

118 Mallu Hemmer Gomes De Menezes

MENEZES. M.H.G. ACUTE AND SUBACUTE TOXICITY OF CAESALPINIA FERREA STEM BARK EXTRACT 126

119.1 Paula Regina Rodrigues Salgado

SALGADO. P. R. R. ANTINOCICEPTIVE ACTIVITY OF CURINE ON THE ACETIC ACID ABDOMINAL CONSTRICTION TEST (WRITHING TEST) IN MICE

127

119.2 Paula Regina Rodrigues Salgado

SALGADO. P. R. R. EVALUATION OF THE ANXIOLYTIC EFFECT OF ETHANOLIC EXTRACT OF SIDASTRUM MICRANTHUM (MALVACEAE) ON THE ELEVATED PLUS-MAZE TEST IN MICE.

128

122 Severino Antônio De Lima Neto VARGAS. M. R. W. DE SCANNING ELECTRON MICROSCOPY (SEM) / ENERGY-DISPERSIVE X-RAY SPECTROSCOPY (EDS) AS AN METHOD FOR THE CHARACTERIZATION OF MICROPARTICLES.

129

123 Gabriel Chaves Neto CHAVES NETO. G1 CLINICAL MODELS AND EVALUATION MEASURES IN THE STUDY OF EXPERIMENTAL ANXIETY 130

125 Ademar Soares Melo MELO. A.S EVALUATION OF THE TYPE ACTION ANTIDEPRESSANT OF THE EXTRACT HYDROALCOHOLIC OF EUPATORIUM AYAPANA VENT.

131

126 Yamara Arruda Silva De Menezes

MENEZES. Y. A. S.1 EXTRACTION. MEASUREMENT AND ANALYSIS OF TOTAL PROTEIN OF NATIVE PLANTS FROM RN 132

128 Mayara Marilia Ferreira Limeira LIMEIRA. M.M.F. REPEAT-DOSE TOXICITY OF THE AQUEOUS EXTRACT OF CAESALPINIA FERREA STEM BARK 133

129 Marco Navarro NAVARRO. M. V. M. PRELIMINARY STUDIES FOR THE USE OF ARTIFICIAL NEURAL NETWORKS (ANN) IN THE STUDY OF RHEOLOGIC PROPERTIES OF TUBERCULOSTATICS AND SOLID EXCIPIENTS

134

130 Alexandre Medeiros Dantas DANTAS. A.M. GASTROPROTECTIVE EFFECTS OF ETHANOLIC EXTRACT FROM SPONDIA PURPUREA ON EXPERIMENTAL GASTRIC ULCER MODELS IN RODENTS

135

131 Romélia Pinheiro Gonçalves BANDEIRA. I.C.J IMPACT ON HEMATOLOGICAL PARAMETERS OF L-ARGININE SUPPLEMENTATION IN PATIENTS WITH SICKLE CELL ANEMIA IN USE OF HYDROXYUREA

136

132 Izabel Cristina Justino Bandeira BANDEIRA. I.C.J ALTERAÇÕES HEMATOLÓGICAS EM CRIANÇAS COM TETRALOGIA DE FALLOT SUBMETIDAS À CIRURGIA CARDÍACA COM CIRCULAÇÃO EXTRACORPÓREA

137

133 Thiago Peixoto Rodrigues RODRIGUES. T.P PROFILE OF PATIENTS WITH CHRONIC DISEASE NON-TRANSMISSIBLE PARTICIPANTS OF A PHARMACEUTICAL CARE PROGRAM

138

134 Gabriel Azevedo De Brito Damasceno

DAMASCENO GAB PREPARATION AND CHARACTERIZATION OF INCLUSION COMPLEXES BETWEEN CHLORPROPAMIDE AND Β-CYCLODEXTRIN

139

135 Maria Aline Barros Fidelis De Moura

MIRANDA. N. TOXICOLOGICAL PROFILE OF EMERGENCY ATTENDED AT VIÇOSA-AL HOSPITAL 140

136 Gabriel Araujo Da Silva ARAUJO-SILVA. G. DEVELOPMENT AND VALIDATION OF HPLC FAST METHOD FOR SIMULTANEOUS DETERMINATION OF NINE POLYPHENOLS COMPOUNDS IN CRUDE EXTRACTS OF PLANTS

141

137.1 Denise Michelle Costa Barros BARROS. D.M.C. APPLICATION OF DIFFERENTIAL THERMAL ANALYSIS (DTA) TO DETERMINE OF TRIOXSALEN-EXCIPIENT COMPATIBILITY

142

137.2 Denise Michelle Costa Barros BARROS. D.M.C. COMPATIBILITY STUDY OF VITAMIN D3 AND MANNITOL IN PHYSICAL MIXTURES 143

138 Jonas Ivan Nobre Oliveira OLIVEIRA. J. I. N A QUANTUM STUDY OF DRUG-RECEPTOR INTERACTION: RECONHECENDO AS BINDING ENERGIES BETWEEN 16S RRNA RESIDUES AND HYGROMYCIN B

144

140 Yonara Monique Da Costa Oliveira

OLIVEIRA. Y.M.C STUDY OF 599 C>T POLYMORPHISM OF THE GPX1 GENE IN TYPE 1 DIABETES: IMPLICATIONS IN ANTIOXIDANT DEFENSE AND DIABETIC NEPHROPATHY

145

141.1 Thereza Mylene PEREIRA. T. M. M PRELIMINARY STUDY FOR THERMAL CHARACTERIZATION OF BIOIDENTICAL HORMONE ESTRADIOL 146

141.2 Thereza Mylene PEREIRA. T. M. M LINEARITY IN DTA METHOD: A COMPARISON OF TWO QUANTIFICATION STRATEGIES OF BIOIDENTICAL HORMONE

147

142 Walteçá Louis Lima Da Silveira SILVEIRA. W. L. L. INTERFERENCE ON ADHERENCE OF CANDIDA SSP IN CONTACT LENSES BY MICROEMULSION SYSTEMS 148

143.1 Thays Oliveira OLIVEIRA. T.S. CHARACTERIZATION OF THERMAL EVENTS OF HYDROQUINONE DEPIGMENTANT BY DTA 149

143.2 Thays Oliveira OLIVEIRA. T.S. COMPATIBILITY STUDY OF TRETINOIN AND EMULSIFYING AGENT IN PHYSICAL MIXTURES 150

144 Fabrício Alexopulos Ferreira MELO. A.S. EVALUATION OF THE ANTINOCICEPTIVE EFFECT OF HYDROETANOLIC EXTRACT OF PORTULACA PILOSA L (PORTULACACEAE). IN MICE

151

145 Mara Rúbia Winter De Vargas VARGAS. M. R. W EVALUATION OF PHYSICOCHEMICAL PROPERTIES OF POORLY WATER SOLUBLE DRUG (SIMVASTATIN) OBTAINED BY RECRYSTALLIZATION

152

146 Erlhane Irineu Lourenço Silva SILVA; E.I.L ANALYSIS AND CHARACTERIZATION OF OILS AND FATS 153

148 Franklin Ferreira De Farias Nóbrega

NÓBREGA. F.F.F. EVALUATION OF ANTIOXIDANT ACTIVITY IN SEASONAL STUDY OF PHASE ETHYL ACETATE BAUHINIA CHEILANTHA (BONG.) STEUDEL

154

149.1 Renata Cristina De Araujo Valença

VALENÇA. R.C.A PHARMACEUTICAL ASSISTANCE THE ELDERLY: SHARES OF COMMUNITY INTERVENTION IN BRASILIA TEIMOSA 155

149.2 Renata Cristina De Araujo Valença

VALENÇA. R.C.A PROTEIN ANALYSIS BY BRADFORD METHOD IN SAMPLES OF CEREAL 156

150 Gabriela Muniz Felix Araújo ARAÚJO. G. M. F. ANTIMICROBIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF STRYPHNODENDRON ADSTRINGENS MART. AGAINST STRAIN OF STAPHYLOCOCCUS AUREUS

157

151 Kátia Lira Da Silva SILVA. K. L. STUDIES OF MAGNETIC PARTICLES PREPARED BY CO-PRECIPITATION UNDER MAGNETIC FIELD FOR DRUG DELIVERY

158

152 Jéssica Layanne Cabral Azevedo

AZEVEDO. J. L. C A STRUCTURAL AND ENERGETIC INVESTIGATION OF PAROMOMYCIN-16S RRNA COMPLEX 159

153 João Ricardo P. De Carvalho CARVALHO. J.R.P SYNTHESIS AND CHARACTERIZATION OF AMOXICILLIN AND METRONIDAZOLE-BASED CHITOSAN MICROPARTICLES

160

154 Tulio Salgado SALGADO. T.M.M VISCERAL LEISHMANIASIS: SURVEY OF NOTIFIED CASES OF DISEASE IN THE CITY OF ASSU / RN 161

156 Wendell M. De Azevedo. AZEVEDO. W. M. DE OZAWA CALCULATION APPLIED TO THE KINETIC STUDY OF DECOMPOSITION OF FLUCONAZOLE BY THERMOGRAVIMETRY

162

157.1 Edilamar Pereira Da Silva SILVA E. P. THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE014 BY TG/DTA AND DSC 163

157.2 Edilamar Pereira Da Silva GALVÃO. F. P DETERMINATION OF KINETIC PARAMETERS BY OZAWA OF ALKALOID SYNTETIC WE014 164

158 Rachel Ouvinha De Oliveira OLIVEIRA R. DEVELOPMENT OF GOLD NANOPARTICLES CONTAINING DOCETAXEL 165

160 Artur Felipe Costa Amorim AMORIM. A. F. C EVALUATION IN VITRO OF SCHINUS TEREBINTHIFOLIUS ANTIOXIDANT ACTIVITY BY CAPTURE OF FREE RADICAL 2.2-DIFENIL-1-PICRILIDRAZIL (DPPH)

166

161.1 Karla Monik Alves Da Silva SILVA. K. M. A. ANTIMICROBIAL ACTIVITY OF ANNONA CORIACEA L. AGAINST ORAL CAVITY BACTERIA 167

161.2 Karla Monik Alves Da Silva SILVA. K. M. A. MINIMUM INHIBITORY CONCENTRATION OF ACANTHOSPERMUM HISPIDUM DC FRONT OF STREPTOCOCCUS ORALIS BYTHE MICRODILUTION AND AGAR DIFFUSION METHODS

168

162.1 Laianne Carla Batista Alencar ALENCAR. L.C.B. ANTIMICROBIAL ACTIVITY OF ACANTHOSPERMUM HISPIDUM DC. BY MICRODILUTION 169

162.2 Laianne Carla Batista Alencar ALENCAR. L.C.B. MICROBIOLOGICAL COMPARISON OF HYPTIS MUTABILIS BRIQ ACTION. BY THE MICRODILUTION AND AGAR DIFFUSION METHODS

170

163.1 Fernando José De Lima Ramos Júnior

RAMOS JÚNIOR. F. J. L. DIAGNOSIS OF HYGIENIC-SANITARY CONDITIONS AND COMPLIANCE WITH GOOD MANUFACTURING PRACTICES IN FOOD SERVICE AT PUBLIC DAY CARE CENTERS IN CAMPINA GRANDE-PB

171

163.2 Fernando José De Lima Ramos Júnior

RAMOS JÚNIOR. F. J. L. PREVALENCE OF INTESTINAL PARASITES AMONG STUDENTS OF PUBLIC DAY CARE CENTER IN THE CITY OF CAMPINA GRANDE-PB

172

164 Jamilly Kelly Oliveira Neves APOLINÁRIO. A.C. FORMULATION AND EVALUATION OF SUNSCREEN EMULSIONS USING EXPERIMENTAL DESIGN 173

165.1 Maria Luiza Carneiro Moura MOURA. M.L.C INTRINSIC DISSOLUTION: THE INFLUENCE OF THE TECHNIQUE ON THE KINETIC PARAMETERS EVALUATION OF ANTIRETROVIRAL EFAVIRENZ

174

165.2 Maria Luiza Carneiro Moura MOURA. M.L.C INTRINSIC VERSUS APPARENT DISSOLUTION: INFLUENCE OF COMPRESSION ON THE KINETIC EVALUATION OF ANTIRETROVIRAL EFAVIRENZ

175

167 Joedna Cavalcante Pereira PEREIRA. J.C. INVESTIGATION OF THE SPASMOLYTIC ACTION OF SOLANUM STRAMONIFOLIUM JACQ. ON RAT AORTA AND GUINEA-PIG ILEUM

176

168 Vanessa Duarte De Almeida ALMEIDA. V. D. DE KINETIC STUDIES FOR ITRACONAZOLE USING OZAWA MODEL BY DYNAMIC THERMOGRAVIMETRY 177

169 Mariana Angélica Oliveira Bitencourt

BITENCOURT. MAO PHYTOCHEMICAL STUDY AND ANTI-INFLAMMATORY ACTIVITY OF AQUEUS EXTRACTS AND FRACIONS OF MIMOSA TENUIFLORA (JUREMA PRETA) AND HANCORNIA SPECIOSA (MANGABEIRA)

178

170 Jahamunna Abrantes Andrade Barbosa

BARBOSA. J. A. A. THERMAL ANALYSIS MULTCOMPONET COMPLEX WITH Β-CD AND METHOTREXATE 179

171 Dayanne Tomaz Casimiro Da Silva

SILVA. D.T.C. PSEUDO-TERNARY PHASE DIAGRAMS AS A TOOL TO PRODUCE EMULSION E MICROEMULSION 180

172 Rayanne Sales De Araújo Batista

BATISTA. R. S. A. DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF HYDROALCOHOLIC EXTRACT PLANT ON CANDIDA SPECIES OF MEDICAL INTEREST

181

174 Adriano Francisco Alves ALVES. A. F THE CURINE ANTIINFLAMMATORY EFFECT IS A CYTOKINE DEPENDENT MECHANISM. 182

175.1 Rayllan Rodrigues RODRIGUES. R. O. DTA/TGA/DTG ANALYSIS OF EUTERPE OLERACEA (AÇAÍ) GLYCOLIC EXTRACT 183

175.2 Rayllan Rodrigues RODRIGUES. R. O. KINETICS STUDIES OF UVB SUNSCREENS: ETHYLHEXYL TRIAZONE AND ETHYLHEXYL METHOXYCINNAMATE 184

176 Ariane Priscilla M. Tenório TENÓRIO. A. P. M LECTIN ANTINOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY EXTRACTION. PURIFICATION AND EVALUATION OF CANNA LIMBATA ROSCOE SEEDS

185

177.1 Ipojucan Silva Fontes FONTES. I. S. RHEOLOGICAL ANALYSIS IN PRELIMINARY STABILITY STUDIES OF SUNSCREEN EMULSIONS CONTAINING AÇAI EXTRACT (EUTERPE OLERACEAE).

186

177.2 Ipojucan Silva Fontes FONTES. I. S. EVALUATION OF SUNSCREENS THERMAL ANALYSIS BY THERMOANALYTIC TECHNIQUES 187

179 Melina Bezerra Loureiro NENHUM AUTOR SUBLINHADO

STUDY OF ASSOCIATION BETWEEN OPG POLYMORPHISMS (- 163A>G; – 1181G>C) AND MRNA EXPRESSION IN TYPE 1 DIABETES PATIENTS

188

180 Denise Porfirio Emerenciano EMERENCIANO. D.P. THIRD ORDER EXPERIMENTAL APPROACH FOR HUMIDITY DETERMINATION IN THE LEAVES OF AZADIRACHTA INDICA A. JUSS

189

181 Heglayne Pereira Vital Da Silva SILVA. H.P.V HUMAN LEUCOCYTE ANTIGEN REGION AND DIABETIC NEPHROPATHY IN CHILDREN AND ADOLESCENTS WITH TYPE I DIABETES OF RIO GRANDE DO NORTE. BRAZIL

190

182 Layany Carolyny Da Silva Mourão


PREPARATION AND CHARACTERIZATION OF INCLUSION COMPLEXES FORMED OF ESSENTIAL OILS OF LIPPIA SIDOIDES CHAM AND LIPPIA ORIGANOIDES HBK WITH Β-CYCLODEXTRIN

191

183 Karla Simone Costa De Souza SOUZA. K.S.C ASSOCIATION BETWEEN IGF-1 (-1245G>A). TGF-Β1 (-509C>T) AND LRP5 (126188 C>T) POLYMORPHISMS IN THE DEVELOPMENT TYPE 1 DIABETES

192

184 Daiane Rafaela Meira Ribeiro Batista

BATISTA. D.R.M.R

VALIDATION OF UV – SPECTROPHOTOMETRIC ANALYTICAL METHOD FOR THE DETERMINATION OF PRAZIQUANTEL IN INCLUSION COMPLEXES WITH Β-CYCLODEXTRIN IN PRESENCE OF HYDROXYPROPYLMETHYLCELLULOSE

193

185 Menilla Maria Alves De Melo MELO. M.M.A. EVALUATION OF COMPONENTS OF METABOLIC SYNDROME IN ADOLESCENTS WHO ARE OVERWEIGHT ACCORDING TO THE NATIONAL CHOLESTEROL EDUCATION PROGRAM - ADULT TREATMENT PANEL III

194

186 Jéssica De Fátima Vianna VIANNA. J. F ENERGETIC ASPECTS OF CHEMICAL INTERACTION BETWEEN THE VIOMICYN ANTIBIOTIC AND BACTERIAL RIBOSOME

195

187 Raquel De Melo Barbosa BARBOSA. R. M VALIDATION OF A HPLC METHOD FOR THE QUANTIFICATION OF DIBUCAINE IN SOLUTION AND ENCAPSULATED IN SOLID LIPID NANOPARTICLES OR NANOSTRUCTURED LIPID CARRIERS

196

188.1 Itavielly Layany França Feitosa FEITOSA. I. L. F EVALUATION OF ANTIBACTERIAL POTENTIAL OF THE LEAVES OF SENNA OCCIDENTALIS (L.) LINK 197

188.2 Itavielly Layany França Feitosa FEITOSA. I. L. F EVALUATE OF THE ANTIFUNGAL ACTIVITY OF SENNA OCCIDENTALIS (L.) LINK.’S LEAVES FORWARD TO STRAINS OF CANDIDA SPP

198

189 Karla Samara Rocha Soares. SOARES. K.S. R CHITOSAN NANOPARTICLES FOR PROTEIN DELIVERY OBTAINED BY IONIC GELATION 199

190 Melissa Alexandra Da Rosa Costa

COSTA. M.A.R CLINICAL-EPIDEMIOLOGICAL PROFILE OF PATIENTS WITH BREAST CANCER IN TO THE COMBINATION CANCER CHEMOTHERAPY 5-FLUOROURACIL. EPIRUBICIN AND CYCLOPHOSPHAMIDE

200

191.1 Geisa Nobre Oliveira OLIVEIRA. G. N. ATIMICROBIAL ACTIVITY OF BIOACTIVE METABOLITES ISOLATED FROM STREPTOMYCES SPP. AGAINST PATOGENIC FUNGI YEAST

201

191.2 Geisa Nobre Oliveira OLIVEIRA. G. N. ACUTE TOXICITY OF CARVACROL 202

192 Liliane De Queirós Souza SOUZA. L.Q.S. EVALUATION OF THE TOXICITY OF PRADOSIA HUBERI HYDROALCOHOLIC EXTRACT ON THE HYPOPHYSIS-GONADAL AXIS OF PREGNANT FEMALE RATS

203

193 Deysiane Oliveira Brandão BRANDÃO. D. O. DRUG PROFILE OF ELDERLY DIABETES AND DYSLIPIDEMIA ATTENDED BY PHARMACEUTICAL CARE PROGRAM IN A COMMUNITY PHARMACY

204

194 Paulo Ricardo Silva Pereira PEREIRA. PRS EVALUATION OF HEMATOPOIESIS IN MALNOURISHED MOUSE MODEL 205

195 Raquel Araújo De Oliveira OLIVEIRA. R. A REPORTING OF DRUGS INDICATORS OF ADVERSE DRUG REACTIONS: STUDY IN A UNIVERSITY HOSPITAL 206

196 Gustavo Henrique De Medeiros Oliveira

OLIVEIRA. G.H.M. STUDY OF TAQI/TGFΑ POLYMORPHISM IN NON-SYNDROMIC CLEFT LIP AND/OR PALATE 207

197 Maryanne Melo. MELO. M. N. PHYLLANTUS NIRURI L. REDUCE DAMAGE IN THE MUCOSAL IN MODEL OF COLITIS 208

198 Janaina Da Silva Goes GOES. J. S. COMPATIBILITY BETWEEN CAPTOPRIL AND EXCIPIENTS THROUGH THERMAL ANALYSIS 209

199 José Izak Ribeiro De Araújo ARAÚJO. J.I. R INTESTINAL PARASITES IN CHILDREN AND FOOD MANIPULATORS OF SCHOOL AND RESIDENTIAL OF A MUNICIPAL NURSERY IN JOÃO PESSOA- PB

210

200.1 Evelyn Mirella Lopes Pina PINA. E. M. L CHARACTERIZATION OF SPRAY-DRIED PRODUCT AND LIOPHILIZED DRIED EXTRACT OF BAUHINIA FORFICATA LINK BY INFRARED SPECTROSCOPY

211

200.2 Evelyn Mirella Lopes Pina PINA. E. M. L EVALUATION OF INTERFERING LIPOPHILIC ON DETERMINATION OF CONTENT OF TOTAL FLAVONOIDS BY SOLID PHASE EXTRACTION

212

201.1 Monize Santos Peixoto PEIXOTO. M.S PHYSICAL CARACTERIZATION OF SPRAY-DRIED PRODUCT AND LIOPHILIZED DRIED EXTRACT OF BAUHINIA FORFICATA LINK BY SCANNING ELECTRON MICROSCOPY (SEM) AND X-RAY DIFFRACTION.

213

201.2 Monize Santos Peixoto PEIXOTO. M.S EVALUATION OF HYGROSCOPIC IN SPRAY-DRIED PRODUCT (SDP) AND LYOPHILISATE DRIED EXTRACT (LDE) OF LEAVES OF BAUHINIA FORFICATA LINK

214

202 Rafael Jorge Melo De Omena OMENA. RAFAEL JORGE MELO

SYNTHESIS. CHARACTERIZATION AND EVALUATION OF THE LEISHMANICIDAL ACTIVITY OF MEGLUMINE ANTIMONIATE IN PROMASTIGOTE FORMS OF LEISHMANIA MAJOR

215

203 Nataly Miranda Do Nascimento NASCIMENTO. N. M. INTERACTIONS OF ANTI-HYPERTENSIVE THERAPY DISPENSED BY THE MUNICIPAL HEALTH NETWORK OF MACEIO / AL

216

204.1 João Paulo Tavares Malheiro MALHEIRO. J. P.T USE OF STATISTICAL PROCESS CONTROL AS A TOOL IN OPTIMIZING THE QUALITY OF THE PRODUCTION OF DIPYRONE 500 MG/ML ORAL SOLUTION DROPS

217

204.2 João Paulo Tavares Malheiro MALHEIRO. J. P.T ENVIRONMENTAL MONITORING IN THE FILLING LINE DROPS: AN ANALYSIS OF THE ENVIRONMENT. PRODUCTIN PROCESS AND FINISHED PRODUCT DIPYRONE 500MG

218

205.1 Fabíola Lélis De Carvalho CARVALHO. F. L. PRELIMINARY EVALUATION OF THE ANTINOCICEPTIVE ACTIVITY OF THE SESQUITERPENE NEROLIDOL 219

205.2 Fabíola Lélis De Carvalho CARVALHO. F. L. SEROLOGICAL STUDY OF CHAGAS DISEASE IN THE CITY OF SALGADINHO/PB 220

206 Alan Leite Moreira. MOREIRA. A.L. EVALUATION OF PHYSICAL AND CHEMICAL COMPOSITION OF THREE CULTIVARS OF PAPAYA SEEDS PRODUCED IN THE NORTHEAST OF BRAZIL FOR POSSIBLE RECOVERY IN FOOD INDUST

221

207.1 Sarah Rafaelly Araújo Santos SANTOS. S.R.A. EXTRACTION AND CHARACTERIZATION OF CHITIN FROM SHRIMP WASTE OF RIO GRANDE DO NORTE - BRAZIL 222

207.2 Sarah Rafaelly Araújo Santos SANTOS. S.R.A. EVALUATION OF THE AUTHENTICITY OF A SAMPLE OF SENNA MARKETED IN NATAL-RN. 223

208.1 Matheus Angêlos ANGELOS. M.A. EVALUATION OF QUALITY OF SAMPLES OF BACCHARIS TRIMERA MARKETED IN DIFFERENT CAPITALS OF THE BRAZILIAN NORTHEAST THROUGH HPLC ANALYSIS

224

208.2 Matheus Angêlos ANGELOS. M.A. ANALYSIS OF EXTENDED RELEASE TABLETS BENZNIDAZOLE BY RAMAN SPECTROSCOPY AND MICROSCOPY 225

209.1 Fabiana Paiva Galvão GALVÃO. F. P DETERMINATION OF KINETIC PARAMETERS BY OZAWA FOR ALKALOID SYNTETIC WE010 226

209.2 Fabiana Paiva Galvão GALVÃO. F. P THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE010 BY TG/DTA AND DSC 227

210 Monique Dielly JANUÁRIO. M.D.A. PARTICIPATION IN CLINICAL PAIN. FOLLOWING THE TREATMENT OF ANTIINFLAMMATORY NON-STEROIDAL ONCOLOGY WARD ASSISTANCE FOUDATION OF PARAIBA – FAP

228

211 Elton Max Nascimento Do Egito

EGITO. E. M. N. THE QUEST FOR ION CHANNEL MEMORY USING A LIPID MEMBRANE 229

213 Tamires Andrade Da Silva NENHUM AUTOR SUBLINHADO

PREPARATION OF DAPSONE-SILICATE NANOCOMPOSITES 230

214 Raul Hernandes Bortolin BORTOLIN. R.H. INFLUENCE OF HIGH GLUCOSE CONCENTRATION ON BONE METABOLISM IN MICE C57BL/6 INDUCED WITH HIGH-FAT DIET

231

215.1 Dayane Beserra Costa COSTA. D.B. CLINICAL AND EPIDEMIOLOGICAL ANALYSIS OF SCORPION STINGS IN CAMPINA GRANDE: PART II 232

215.2 Dayane Beserra Costa COSTA. D.B. SCORPIONIC AND OPHIDIAN ACCIDENT EPIDEMIOLOGIC PROFILE NOTIFIED IN CAMPINA GRANDE CITY 233

216 Mírian Letícia Carmo Bastos BASTOS. M. L. C HYPERTENSION: EDUCATIONAL ACTIVITY IN PUBLIC HEALTH 234

218.1 Leslie Raphael De Moura Ferraz

FERRAZ. L.R.M. ANALYSIS OF DIFFERENT BATCHES OF BENZIDAZOLE BY HPLC AND FTIR 235

218.2 Leslie Raphael De Moura Ferraz

FERRAZ. L.R.M. UTILIZAÇÃO DE TÉCNICAS ESPECTROMÉTRICAS PARA A CARACTERIZAÇÃO DO 3-(4-CLORO-BENZIL)-5-(4-NITRO-BENZILIDENO)-IMIDAZOLIDINA-2.4-DIONA (LFPS/FZ4)

236

220 Mizia Martins COSTA. M. K. C. M. IDENTIFICAÇÃO E SUSCETIBILIDADE ANTIMICROBIANA DE ENTEROCOCOS ISOLADOS NA CIDADE DO NATAL/RN. BRASIL

237

222 Daliana Maria Berenice De Oliveira Souza

SOUZA. DMBO HUMAN LEUKOCYTE ANTIGEN-G AND -E EXPRESSION IN GASTRIC SPECIMENS IN PATIENTS WITH HELICOBACTER PYLORI

238

223 Joanda Paolla SILVA. J. P. R. COST-EFFECTIVENESS OF ANTIHYPERTENSIVE THERAPIES IN BASIC HEALTH SYSTEM 239

224.1 Wende Soares FREITAS. W.S. SURVEY OF RISK FACTORS FOR PREVALENCE OF CASES OF TYPE 2 DIABETES MELITTA IN THE CITY OF ARARA-PB

240

224.2 Wende Soares FREITAS. W.S. EPIDEMIOLOGICAL SURVEY IN CASES OF HEPATITIS TOWN OF CUITE - PB. BRAZIL. 2011. 241

225 Gubbio Matos Da Silva SILVA. G. M. S USE OF BABASSU IN THE ADSORPTION OF CHEMICALS FOR WASTE TREATMENT IN INDUSTRY CHEMICAL AND PHARMACEUTICAL

242

226 Lidyane Neves Miranda MIRANDA. L.N HUMAN LEUKOCYTE ANTIGEN-G AND TH17 EXPRESSION IN CERVICAL INTRAEPITHELIAL NEOPLASIA 243

227.1 Antônia Rosângela Soares Penha

PENHA. A. R. S1 POSSIBLE ANTINOCICEPTIVE EFFECT OF LIPPIA MICROPHYLLA (VERBENACEAE) ESSENTIAL OIL IN MICE 244

227.2 Antônia Rosângela Soares Penha

PENHA. A. R. S1 INVESTIGATION OF ACUTE TOXICITY AND MIORRELAXANT EFFECT OF LIPPIA MICROPHYLLA (VERBENACEAE) ESSENTIAL OIL IN MICE

245

228 Cinthya Maria Pereira De Souza

SOUZA. C.M.P. CHARACTERIZATION OF THE OPUNTIA FICUS-INDICA (L.) MILL DRIED EXTRACT BY THERMAL ANALYSIS AND INFRARED SPECTROSCOPY

246

229 Clélio Diogo Soares NENHUM AUTOR SUBLINHADO

EXPRESSION STUDY OF THE GENES INVOLVED ON FOLIC ACID METABOLISM IN ASSOCIATION WITH ORAL CLEFT DEVOLOPMENT

247

230 Flávia Câmara De Souza SOUZA. F. C PARAMETERS KINETICS BY OZAWA MODEL FOR NICOTINAMIDE BY DYNAMIC TG 248

231 Arsênio Rodrigues Oliveira OLIVEIRA. A.R DEVELOPMENT AND VALIDATION OF A STABILITY INDICATIVE METHOD OF ASCORBIC ACID (VIT C) FOR 500 MG TABLETS

249

232 Laura Da Fonseca Ferreira FERREIRA. L.F DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR QUANTIFICATION OF SIMVASTATIN IN MICROEMULSION BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

250

232.2 Arsênio Rodrigues Oliveira OLIVEIRA. A.R RETROSPECTIVE AND CONCURRENT VALIDATION OF THE MANUFACTURING PROCESS OF ZIDOVUDINE 300MG + LAMIVUDINE 150MG

251

233 Regina Carmen Espósito ESPÓSITO. R.C. A STUDY OF CYTOTOXICITY BY BIOASSAY TECHNIQUES OF THE HOMEOPATHIC PRODUCTS CONTAINING MOMORDICA CHARANTIA

252

234 Thaciane Da Cunha Soares SOARES. T.C UV-VIS ANALYSIS OF CHEMICAL MARKERS OF THE LEAVES OF PASSIFLORA EDULIS SIMS VARIETY EDULIS 253

236 Pedro Henrique Dos Santos Soares

SOARES. P.H.S. ANTICONVULSIVANT AND ANXIOLITIC ACTION EVALUATION OF INDIGOFERA SUFFRUTICOSA MILLS AND INDIGO CARMINE IN MICE

254

237 Lais Farias Azevedo AZEVEDO. L. F DEVELOPMENT OF NEW METHODS OF ANALYSIS AND CONTROL OF METRONIDAZOLE TABLETS USING NEAR INFRARED

255

238.1 Marcelo Amorim AMORIM. M.V.P. CARACTERIZATION OF RIFAMPICIN AND THEIR DEGRADATION PRODUCTS BY CHEMICAL STRESS 256

238.2 Marcelo Amorim AMORIM. M.V.P. COMPARISON STUDY OF TWO ANALYTICALS METHODS OF 4-FDC USING A SINGLE COLUMN 257

239 Leandro Vinicius Fernandes De Morais

MORAIS. L. EVALUATION OF ANALYTICAL METHODS APPLIED TO QUANTIFICATION OF LIPID PEROXIDATION IN LIVER HOMOGENATE OF RATS

258

240 Gabriela Diniz Fonseca FONSECA. G. D. VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF PROPRANOLOL IN CAPSULES USING QUITOSAN AS EXCIPIENT

259

241 Julia Morais Fernandes FERNANDES. J. M. CHROMATOGRAPHIC PROFILE OF THE CRUDE EXTRACT AND FRACTIONS FROM K. BRASILIENSIS CAMBESS (SAIÃO) LEAVES

260

242.1 Cláudia Cecilio Daher DAHER. C. C. KINETCS STUDIES OF EUTERPE OLERACEA (AÇAÍ) EXTRACTS 261

242.2 Cláudia Cecilio Daher DAHER. C. C. SUNSCREENS O/W EMULSIONS STABILIZED BY RHEOLOGICAL MODIFIERS: PRELIMINARY STABILITY TESTS 262

243 José Xavier De Lima Neto LIMA NETO. J. X. STUDY BY QUANTUM MECHANICAL OF INTERACTIONS BETWEEN AMPA RECEPTORS AND FLUORINE WILLARDIINE

263

244 Vanessa Santos Temponi TEMPONI. VS EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL ETHANOLIC EXTRACT FROM VERNONIA POLYANTHES 264

245 Jucelia Barbosa Da Silva. SILVA. J.B EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL ETHANOLIC EXTRACT FROM VERNONIA CONDENSATA 265

246 Vanda Lucia dos Santos SANTOS, V.L EVALUATION OF MUTAGENICITY AND ACUTE TOXICITY IN MICE OF Spondias purpurea (Siriguela) ETHANOLIC EXTRACT.

266

II INTERNATIONAL SYMPOSIUM ON PHARMACEUTICAL SCIENCES:

Challenge and opportunities for development new diagnose methods and pharmaceutical products

1

CODE 1

IMMUNOPHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF HEMATOPOIETIC STEM CELLS AFTER MOBILIZATION IN MALNOURISHED MICE Pereira, P. R. S.1; Silva, B. C. O.1; Avelino, C. U. R.1; Cavalcanti Junior, G. B.2; Borelli, P.3; Schimieguel, D. M.1 1Laboratório de Hematologia Clínica; Departamento de Análises Clínicas e Toxicológicas/CCS/UFRN. 2Laboratório de Imunologia Clínica; Departamento de Análises Clínicas e Toxicológicas/CCS/UFRN. 3Faculdade de Ciências Farmacêuticas/ Departamento de Análises Clínicas e Toxicológicas/USP. Corresponding author: [email protected] Introduction: Hematopoietic stem cell transplantation (HSCT) is a therapy performed in persons with hematologic malignancies. Recruitment of stem cells from the bone marrow (BM) into peripheral blood (PB) is termed mobilization. Bone marrow hypoplasia has generally treated intensively may decrease the effectiveness of therapy, similar to that found in malnutrition. Objectives: To assess the functional status and immunophenotypic profile of hematopoietic stem cells after mobilization in a murine model of malnutrition and bone marrow hypoplasia. Materials and Methods: We studied eight adult male BALB/c separated in two groups: malnourished (D) and control (C). The group D (n = 5) received low-protein diets for five weeks, and group C (n = 3) received normal protein diets ad libitum. All animals were mobilized with G-CSF fo 5 d ys dose 100μg/Kg/d y. On sixth day, animals were euthanized to obtain PB and BM, by washing the medullary cavities of femurs and tibias. BM samples were cultured in methylcellulose plus growth factors (IL-3, G-CSF, GM-CSF) for functional evaluation by counting the colony-forming units (CFU); G-CFU and GM-CFU. Quantification of HSC was performed by immunophenotyping (CD34+ and Sca-1+). Results: There was no significant difference in quantification of HSC present in BM/PB between the two groups. Total CFU and CFU-G were significantly decreased in samples of group D than in group C (p = 0, 0357). Conclusion: There was no difference in amount of cells mobilized from the BM niche of malnourished group. However, functionally the HSC seem somehow damaged, with reduced differentiation and proliferation. Financial support from UFRN.



2

CODE 2 IN VITRO ACARICIDAL AND CYTOTOXIC ACTIVITY OF AQUEOUS

EXTRACTS OF Agave sisalana PERRINE AND Furcraea selloa K. KOCH Macedo, A. L.1; Prata, M. C. A.2; Chedier, L. M.3; Sousa, O. V.4; Pimenta, D. S.3; Pires, M. F. A.2.

1Ph m ceu c sc ences m s e ’s s uden , UFJF. 2Embrapa Dairy Cattle researchers. 3Botanical Department, Biologic Sciences Institute, UFJF. 4Pharmaceutic Department, Pharmacy College, UFJF. Corresponding author: [email protected] Introduction: Agave sisalana Perrine e Furcraea selloa K. Koch, part of Ag v ce e f m y nd popu y known s “p ”, h ve been used s pesticides and antimicrobials agents in ethnoveterinary, especially as acaridal. Objective: Evaluate the acaricial and cytotoxic activities of the leaves infused of A. sisalana and F. selloa. Material and Methods: F. selloa and A. sisalana leaves were collected in Juiz de Fora, Minas Gerais, Brazil. The aqueous extract was obtained by infusion of the dried and crushed leafs. Acaricidal activity was evaluated against Rhipicephalus (Boophilus) microplus at extracts using the extracts in concentration ranging of 40mg/mL to 200mg/mL. The citotoxic activity was tested against Artemia

salina exposed to extracts in concentrations ranging of 10 to 1000 g/mL. Results and Discussion: Extracts don’ n e fe ed s gn f c n y n y ng and hatching, but A. sisalana extract showed a light increase in the laying and the F. selloa extract showed a light decrease of the same. The test population showed resistance to pyrethroids, organophosphates and m d nes s ng y nd n ssoc on. The ex c s we en’ ox cs g ns A. salina in the tested concentrations, having F. selloa killed 22,5% and A. sisalana 10,0% of the nauplii in the higher concentration. These results corroborate with previous publication were the acaricidal activity were associated with the cytotoxicity probably linked with the saponins presents in these species. Conclusion: The obtained results indicate that the leaves nfused of bo h spec es don’ h ve c c d nd c o ox c c v es. Nex studies should focus on the leaves methanolic extracts of F. selloa. Financial support from FAPEMIG and master scholarship from Embrapa dairy cattle.



3

CODE 3 PRELIMARY STUDY OF BORNEOL SALICILATE ANTIINFLAMATORY

ACTIVITY Vasconcelos, R. M. C.¹; Leite, J. A.; Leite, F. C.¹; Rodrigues, L. C.²; Piuvezam, M. R.¹ ¹Laboratório de Imunofarmacologia, Centro de Biotecnologia, Universidade Federal da Paraíba (UFPB). ²Laboratório de Farmacoquímica, Centro de Biotecnologia, Universidade Federal da Paraíba (UFPB). Corresponding author: [email protected] Introduction: Borneol Salicilate (BoS) is a synthetic compound obtained by esterification reaction of salicylic acid and (-)-borneol alcohol. The diastereomer (-)-borneol has been described as a potential anti-inflammatory molecule in neuronal cells. Objective: The goal of this study was to evaluate BoS in murine models of inflammation. Material and Methods: The experimental models used were carragenaan-induced paw edema and zymosan-induced peritonitis. Swiss mice (n=6) orally pretreated with 50, 100 or 200 mg/kg BoS or 200 mg/kg acetylsalicylic acid (AAS) as control drug rece ved n njec on of 20 μL of c gen n (2,5%) in left paw and NaCl 0,9% solution in right paw. The paw volume was measured in different times to evaluate the edema formation. In the peritonitis experimental model, pretreated animals (n=8) received an n pe one njec on of 500 μL of zymos n (2 mg/mL) nd 4 hou s f e the peritoneum was exposed and the fluid was collected with 1 mL ice-cold PBS. The aspirate was centrifuged and the cells were ressuspended and counted in hemocitometer. Results and Discussion: The pretreatment with BoS (200 mg/kg) was significantly (p<0.05) effective in inhibiting the paw edema in 1h, 2h, 6h and 24h, as well as the AAS; however, the minor doses of BoS were not effective. The BoS (200 mg/kg) also reduced significantly (p<0.05) the neutrophil migration to peritoneum. Conclusion: These preliminary data suggest that BoS has anti-inflammatory effects related with eicosanoid mediators. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil

mailto:[email protected]



4

CODE 4 ESTIMATION OF CARDIOVASCULAR RISK BY THE FRAMINGHAM

SCORE IN HYPERTENSIVE PATIENTS Portela, A. S.1,2; Silva, P. C. D.2; Falcão, R. A.2; Malheiro, J. P. T.2; Montenegro Neto, A. N.2; Almeida, M. G.1,3; Simões, M. O. 2

1Programa de Desenvolvimento e Inovação Tecnológica de Medicamentos, Universidade Federal do Rio Grande do Norte (UFRN). 2Laboratório de Pesquisa em Bioquímica Clínica, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 3Laboratório Multidisciplinar, Departamento de Análises Clínicas e Toxicológicas, UFRN. Corresponding author: [email protected] Introduction: Cardiovascular diseases are the leading cause of death in

Brazil. The main risk factors for these diseases are Arterial

Systemic Hypertension, dyslipidemia, smoking and Diabetes

Mellitus (DM). Material and Methods: Based on this framework, this

study aims to evaluate the cardiovascular risk of hypertensive

patients by the Framingham risk score. It is a cross-sectional study which

included a random sample of 53 individuals with hypertension treated at

the Municipal Health Service of Campina Grande, in Paraíba. It was

adopted the Framingham score, taking into account the sum

of points obtained from the set of risks, from the age, presence of

diabetes, the practice of smoking, blood pressure and the ratio

between total cholesterol and HDL. Results and Discussion: From the

results, it was observed that 81.25% of the male population had risk equal

or more than 10% for cardiovascular complications estimated at 10

years, according to Framingham scores. In women, on the contrary,

statistical analysis showed that this risk was 76.47% less than or equal to

8%. Conclusion: The results showed the importance of implementing

the score in Primary Care, which allow for early diagnosis of people at risk

and identify remedial measures that may be effective in treating

and preventing cardiovascular events, many of them fatal, avoiding

personal suffering and reducing costs to the health system and society.

Financial support: Incentive Program for Graduate Studies and Research (PROPESQ) UEPB




5

CODE 5 CHARACTERIZATION OF A CATIONIC NANOEMULSION INTENDED

TO GENE THERAPY AFTER STERILIZATION PROCESSES Silva, A. L.¹,²; Alexandrino-Junior, F.¹,²; Silva, K. S.¹; Veríssimo, L. M.¹,²; Lima, L. F. A.²; Egito, E. S. T.¹ ¹Laboratório de Sistemas Dispersos (LaSiD), Departamento de Farmácia (UFRN); ²Laboratório de Biologia Molecular e Genética (LBMG), Centro de Biociências (UFRN). Corresponding author: [email protected] Introduction: Gene therapy is based on the transfer of genetic material into the target defective tissue or cell. For its success, it is necessary to develop carriers able to bypass both, membrane and citosol barriers, reaching the nucleus. Most of the researchers deals with viral approaches. However, concerns about safety have increased studies on non viral gene delivery systems like nanoemulsions. Objective: The aim of this work was to evaluate autoclaving and filtration as methods to guarantee a cationic nanoemulsion system sterility, which makes in vitro assays possible. Material and Methods: The nanoemulsion, prepared by sonication method, was made of Captex 355®, Span 80®, Tween 80®, water and stearylamine. Sterilization processes was done as follow: filtration by 0.22 µm filter and autoclaving for 15 min at 1 atm and 121°C. Then, characterization by macroscopic analyses, zeta potential and size measurements were performed. Results and Discussion: The results showed that, as expected, phase separation happens when autoclaving was used to sterilize the nanoemulsion (a thermodynamically unstable system). Concerning the filtration method, the characteristics of the system were maintained. The unsterilized nanoemulsion presented size of 240 nm and zeta potential value of +26.6 mV. After filtration, the system presented 233 nm of size and +23.9 mV of Zeta Potential. Polidispersivity Index decreased from 0.201 to 0.176 after filtration, meaning that size distribution was more homogenous after the filtration process. Conclusion: These results demonstrate that sterile filtration is a suitable process to sterilize cationic nanoemulsion for gene therapy making possible the future use of cationic nanoemulsions for gene delivery.




6

CODE 6 SV1: ONE POLYSACCHARIDE POPULATION FROM Sargassum

vulgare WITH ANTICOAGULANT AND ANTITHROMBOTIC ACTIONS AND THROMBIN INHIBITOR Dore, C. M. P. G.1; Alves, M. G. C. F.1; Medeiros, H. N.1; Paiva, A. A. O.1; Filgueira, L. G. A.1; Leite, E. L.1

1Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN). Introduction: The activation of the blood coagulation cascade results in

the formation of the factor Xa which consequently catalyzes the formation of thrombin. Thrombin converts fibrinogen to fibrin, which polymerizes to form a clot. Sulfated fucans (fucoidans) were isolated of species from brown seaweed showing anticoagulant activity. Objective: In this study

was evaluated the anticoagulant action of fucans extracted from Phaeophyceae, Sargassum vulgare, investigating its possible course of action on blood anticoagulation. Material and Methods: The algae S.

vulgare was collected on the southern coast of Rio Grande do Norte, washed, processed,delipided and isolated with increasing concentrations of acetone, obtaining a sulfated polysaccharide called F1.0 fraction. This compound was chemically characterized and its effect on the extrinsic and intrinsic coagulation tests was performed by the thrombin time (PT) and activated partial thromboplastin time (aPTT). In addition, was analyzed the direct effect of polysaccharides on the enzymatic assay of thrombin and factor Xa on its chromogenic substrates, as well as in vivo antithrombotic action of these compounds. Results and Discussion: Evaluation of PT and aPTT

tests showed that the polysaccharides have action on the intrinsic coagulation pathway. The enzyme inhibition assays showed maximum inhibition of thrombin in the order of 50.2% and not demonstrated direct inhibition on factor Xa at 25 µg of polysaccharides. The F1.0 fraction shows strong antithrombotic action with 83.3% inhibition of thrombus formation in vivo. Conclusion: The results suggest that fraction F1.0 has

a direct effect on thrombin as evidenced by its strong anticoagulant and antithrombotic actions.



7

CODE 7 ANTI-INFLAMMATORY AND ANTINOCICEPTIVE ACTIVITIES OF

Phyllanthus niruri L. SPRAY-DRIED EXTRACT Porto, C. R. C.1; Soares, L. A. L.3; Ferreira, A. A. A.2; Souza, T. P.4; Petrovick, P. R.5; Lyra, I. L.6; Gavioli, E. C.2; Guerra, G. C. B.2. 1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte. 2Departamento de Biofísica e Farmacologia, Universidade Federal do Rio Grande do Norte. 3Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco. 4Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas. 5Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul. 6Iniciação científica, Departamento de Biofísica e Farmacologia, Universidade Federal do Rio Grande do Norte. Corresponding author: [email protected] Introduction: Phyllanthus niruri L., a species long used by peoples of tropical and subtropical regions. A large number of preclinical studies have not only confirmed, but also expanded the medicinal uses of species of the Phyllanthus genus mentioned in traditional medicine. Objective: investigate possible anti-inflammatory and antinociceptive effects of Phyllanthus niruri spray-dried extract (PnSDE). Materials and Methods: Two models were used to evaluate anti-inflammatory properties of PnSDE: carrageenan-induced paw edema and thioglycolate-induced leukocyte migration inhibition. Three animal models were use to evaluate antinociceptive effects: Randall Selitto, tail flick and hot plate. Results and Discussion: In carrageenan-induced paw edema, PnSDE (100, 200, 800, and 1600 mg/kg i.p.), evoked considerable edema reduction, suggesting that PnSDE inhibits different chemical inflammation mediators. It was observed that leukocyte migration to the peritoneal cavity was greatly reduced with 100 and 400 mg/kg doses of extract. In the Randall and Selitto test, PnSDE (100 mg/kg and 200 mg/kg i.p.), showed an important peripheral analgesic activity with increased nociceptor threshold to painful stimuli. PnSDE (100 and 200 mg/kg i.p.) in the tail flick and hot plate methods showed significant central analgesic activity, as evidenced by an increase in response time to the thermal stimulus. Conclusions: The PnSDE showed anti-inflammatory properties in both models: leukocyte migration inhibition and paw edema. PnSDE was also able to reduce vascular response in the inflammatory process and inhibit leukocyte migration to the inflammatory site. It can be concluded that PnSDE has antinociceptive effects in antinociceptive models at both peripheral and central levels. Financial support from CNPq/Brazil



8

CODE 8 DETERMINATION OF RALOXIFENE HYDROCHLORIDE USING

ULTRAVIOLET SPECTROPHOTOMETRY AND LC STABILITY-INDICATING METHOD Salazar, F. R.1,*; Bergold, A. M.1

1Laboratório de Química Farmacêutica, Faculdade de Farmácia, UFRGS. Corresponding author: [email protected] Introduction: Raloxifene (RLX) is a selective oestrogen receptor modulator approved in the USA for the prevention of postmenopausal osteoporosis. Due to its relevance and the interesting of INCT- IF to synthesize this molecule, it becomes important to establish methodologies to assure the quality of the pharmaceutical formulation. Objective: The aim of the present work was to develop and validate methods by ultraviolet spectrophotometry (UV) and liquid chromatography (LC) for the determination of RLX in tablets. Materials and Methods: Samples of Evista® were furnished by INCT-IF and reference chemical substance was purchased in the local commerce. The UV method was performed with methanol as solvent and detection at 284nm. The LC analysis used a NST C18 reverse phase 250x4.6mm column, with mobile phase water:acetonitrile:trietilamine (67:33:0.3) pH 3.5 running at flow-rate of 1.0ml/min with temperature of 30°C and detection at 287nm. The methodologies were validated and comparative study was performed. Results and Discussion: Both methods were validated according to ICH guidelines acceptance. The UV method presented linearity in the range of 3.0 – 12.0µg/ml (r=0.9994). Also it presented specificity, precision and accuracy. The LC analysis presented linearity in the range of 4.0-16.0µg/ml (r=0.992). Precision, accuracy and no interference from the degradation products were observed. These methods were compared by statistical n ys s (S uden ’s es ) nd p esen ed no s gn f c n d ffe ences be ween them. Conclusions: The proposed methods were successfully applied for the quantitative determination of RLX. They were statistically equivalent (t-test), assuring the quality of the pharmaceutical dosage form. Financial support from INCT_IF/Brazil and master scholarship from CNPQ/Brazil.



9

CODE 10 AQUEOUS EXTRACT OF Mikania lindleyana DC. PRESENTS

POTENTIAL ANTI-INFLAMMATORY EFFECT Silva, A. S. B.1; Pinheiro, B. G.1; Figueiredo, J. G.3; Souza, G. E. P.2; Cunha, F. Q.3; Lahlou, S. 4; Barbosa, W. L. R.1; Sousa, P. J. C.1

1Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Belém, Pará, Brazil. 2Laboratório de Farmacologia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. 3Departamento de Farmacologia, Faculdade de Medicina, Universidade de São Paulo, Ribeirão Peto, São Paulo, Brazil. 4Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.

Corresponding author: [email protected] Introduction: Mikania lindleyana DC. (Asteraceae), popularly named sucuriju, is a common plant in Brazilian Amazonia. Ethnopharmacological studies show a diversity of uses of this species such in treatment of gastritis, infection, pain and inflammation. Objective: Investigate the effects of aqueous extract of M. lindleyana (AEML) on several models of inflammation in rodents. Materials and Methods: The anti-inflammatory activity was evaluated using the acetic acid in mice, carrageenan-induced paw edema in rats as well as cell migration, rolling and adhesion of leukocytes in carrageenan-induced peritonitis. Results and Discussion: The AEML, at doses 125, 250, 500, 750, 1000 and 1500 mg/kg, significantly inhibited (in a dose-dependant way) the number of contortions induced by acetic acid on writhing test. The median effective dose (ED50) of 692.6 mg/kg was able to inhibit the erythema formation when compared to control group. The AEML inhibited the paw edema carrageenan-induced from 2nd hour and reduced neutrophil migration to peritoneal cavity. Conclusions: Our results indicate that AEML demonstrate an anti-inflammatory activity through inhibition of prodution of inflammatory mediators as PGs, and also on adhesion molecules with involvement of cytokines. Financial support from FAPESP/Brazil and CAPES/Brazil



10

CODE 12 DEVELOPMENT AND VALIDATION OF DOXYCYCLINE HYCLATE BY

HPLC METHOD Kogawa, A. C.1; Salgado, H. R. N.1

1Laboratório de Controle de Qualidade, Departamento de Fármacos e Med c men os, Un ve s d de Es du P u s “Jú o de Mesqu F ho” (UNESP), Araraquara, São Paulo, Brazil Corresponding author: [email protected] Introduction: Doxycycline hyclate (DOX), a broad-spectrum antibiotic, is

part of the Unified Health System (SUS) in Brazil. The HPLC methods described in the USP (2008), British (2010) and Portuguese (2005) Pharmacopoeia are time-consuming. Objective: A simple, sensitive and

accurate HPLC method was developed and validated for the assay of DOX in raw material and tablets. Material and Methods: The method was

developed in a HPLC Waters® composed of pump chromatographic Waters® 1525, manual injector Rheodyne Breeze 7725i and UV-Vis detector Waters® 2487, Luna CN column (250 mm x 4.6 mm), mobile phase water + 0.1% TFA : ACN + 0.1% TFA (60:40, v/v), HCl 0.01M solutions of DOX and wavelength at 360 nm. Results and Discussion: The linearity of the method was proven by the components of parallelism, correlation coefficient, 0.9999, and variance analysis of the results of the analytical curve. The values obtained for the test intraday and interday precision were 1.08 and 0.52%, respectively. The amount of DOX in the samples analyzed, 110.07%, is not consistent with the official compendium that is 95.0 to 105.0%. The accuracy of the method was proven by the recovery test, with an estimated average of 100.18%. The robustness was confirmed by measuring values at wavelength, proportion of mobile phase, injection volume, flow rate and ACN. Conclusion: The developed HPLC

method was considered suitable for quantification of doxycycline hyclate tablets. Regarding chromatographic methods described in the literature, this method demands less time to prepare the mobile phase and has running time of approximately 5 minutes.

Financial support: CAPES, CNPq, FAPESP, UNESP, União Química

Pharmaceutical Industry.



11

CODE 13 STRATEGY FOR TREATMENT OF WATER, FEDERAL UNIVERSITY OF

CAMPINA GRANDE / CENTER FOR EDUCATION AND HEALTH-PB Pinheiro, A. A. V.; Lima, W. S., Araújo, K. A. M.; Alencar, J. S. Farmácia Escola Manoel Casado de Almeida, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG). Corresponding author: [email protected] Introduction: The UFCG/CES-PB offers several courses in education and health. Given the expansion of activities within the CES as the implementation of the Pharmacy College (PC), the University Restaurant, research, among others, it came the need to design a Water Treatment Center (WTC), because the current situation of water is of poor quality for the projected activities. Objective: Therefore, the objective of this work includes the development of the floor plan of the WTC, Standard Operating Procedure (SOP) of the equipment and distribution logistics. The proposed equipment is designed to work during 4h/day 5days/week, providing drinking and purified water according to the law. Material and Methods: The logistics of distribution is designed as follows: water coming from a natural source with a flow rate of 3.000L/h and 2.22mS of conductivity will supply a water tank of 5.000L. This water will be treated by three 5µm pre-filters to remove solids, with a desalination capacity of 99% and three reverse osmosis membranes, acquiring at this stage purified water. This water will go to a 3.000L reservoir and to a 500L conical bottom for the PC, obtaining purified water. Thus, the conical reservoir is coupled to an ultraviolet system, flow of 0.23m3/h, 6w-220volts bacterial protection lamp, and an deionizer of 100L/h with a high degree of chemical purity, obtaining water with a maximum of 1,3μS/cm(25 °C) of conductivity. The monitoring and maintenance of the WTC shall obey the SOP’s developed by students of the PC, which will be routinely revised for answering better the needs of the UFCG/CES.



12

CODE 14 ADVERSE EVENTS IDENTIFIED IN A HOSPITAL OF THE SENTINEL

NETWORK Pereira, A. M. S.1; Silva, M. L. M.1; Porto, M. L. S.2; Farias, A. D.3 1Academic of Pharmacy, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG). 2Msc Pharmaceutical Biochemist, University Hospital Alcides Carneiro, Campina Grande (UFCG). 3Professor of Pharmacy, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG). Corresponding author: [email protected] Introduction: The University Hospital Alcides Carneiro is part of the Sentinel Network of Hospitals, which aims to do the surveillance in health products after they are sold, through the investigation of adverse events in the areas of technical surveillance, pharmacovigilance and haemovigilance. Objective: To identify and analyze adverse events occurred in September-2010/September2011 in that hospital. Material and Methods: It was conducted a retrospective study, through validated questionnaires of active finding from Anvisa, recorded in the period. Results and discussion: We recorded 15.0% of adverse events in haemovigilance, 6.0% of adverse reactions, 38.0% of technical complaints in pharmacovigilance and 41.0% in technical surveillance. In the last one, the largest number of adverse events occurred due to the lack of quality in syringes (63.7%), equipment (12.0%) and urine collection (7.1%). In pharmacovigilance, the parenteral use of isotonic solutions contributed to the higher frequency of events (69.9%). Regarding adverse reactions to medications, dipyrone and ciprofloxacin caused itching and erythematous dermatitis was completed as causal. Hypertension and cyanosis (2.5%) was the most significant reaction to the haemovigilance. It was noted that the events of technical complaints are related to the lack of technical quality in the fabrication of the product and that the most frequently observed adverse reactions were allergic. Conclusions: The reported events bring negative economic consequences for the institution and risks for the patient, requiring a greater involvement of professionals so that can be established a continuous monitoring and thus improve the quality of the treatment with safety to the patient.



13

CODE 15 STANDARDIZATION OF TECHNIQUES FOR EVALUATION OF

HEMATOPOIESIS IN MALNOURISHED MICE Silva, B. C. O.1; Avelino, C. U. R1; Pereira, P. R. S.1; Schimieguel, D. M.1

1 Clinical Laboratory Hematology, Department of Pharmacy of the Federal University of Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Protein-calorie malnutrition (PEM) is a direct result of inadequate dietary protein together with a deficient intake of the carbohydrates and lipids necessary to provide an adequate energy source. Among the most well documented functional consequences of PEM are growth impairment, a reduced immune response, and a disruption in cognitive ability. Moreover, PEM also induces leucopenia, bone marrow hypoplasia and commitment components of the immune response in animal model studies. Objectives: Standardization of anesthetic dosage; standardization of blood sample and bone marrow collection methods, standardization of hematological techniques in peripheral blood; standardization of leukocyte count in samples of bone marrow and induction of malnutrition. Material and Methods: The study employed 14 BALB/c for standardization of anesthesia, peripheral blood (PB) and bone marrow (BM), hematologic determinations, and induction of malnutrition. The animals were submitted to protein malnutrition separated by the control group (CG) and malnourished group (MG). After the initial weight loss, the animals were anesthetized with sodium thiopental with dose of 150 mg/kg for collecting PB and BM. Results and Discussions: The results showed no significant difference between the two groups. Average leukocyte in control group PB:1,45 x103/mm3 and BM: 1,12 x107/mm3; and malnourished group PB: 1,83 x103/mm3 and BM: 9,7 x106/mm3. Parameters of red series (average) in control group: hemoglobin: 12,7g/dL; hematocrit: 45%; erythrocytes: 5,94 x106/mm3. Malnourished group:14,0g/dL hemoglobin, hematocrit 47%, erythrocytes: 4,59 x106/mm3. Conclusion: These preliminary results shows that the test pilots and the standard technique used create ideal experimental conditions to performance and continue the project. Financial support from UFRN/Brazil.




14

CODE 16 REFLECTIONS OF A CUSTODY CHAIN IN CENTER OF

PHARMACEUTICAL EQUIVALENCE

Oliveira, Y. S.¹; Assis, C. N.¹,*; Lopes, C. C. G. O.¹

¹ Department of Pharmacy, Federal University of Ceará (UFC). Corresponding author: [email protected]

Introduction: The Custody Chain is used to maintain and document the

movement of the sample in the laboratory from reception to retain custody

in the documentation and archiving. Objective: Evaluate the results

obtained with the implementation of Custody Chain in order to check if

there is an improvement in the quality system of a Center of

Pharmaceutical Equivalence/CPE and if it is in accordance with the

NBR/ISO/IEC 17025/05. Methods: We adopted tools, including:

preparation of protocols, adoption of master log, logbooks for equipment,

documents and forms. It was also provided training for technical staff to

better use the tools mentioned. The measurement of non-compliance was

accomplished through registration on a form specified and validated.

Results and Discussion: Implementation of Custody Chain benefited the

CPE enabling a more efficient tracking of drugs and providing better

maintenance and calibration of equipments and quality of reagents used.

Using these documents, the CPE can justify the amount of samples

requested for quality control analysis. If any error occurred in the analysis,

it was easier to detect the source. It were observed a considerable

decrease of non-compliances(93,7%) and a faster achievement of analysis

without lost in quality of results, allowing an expansion of the number of

tests and customers. There was an increase of credibility with ANVISA and

customers; resulting from the work according to the standards.

Conclusion: The custody chain is a valuable resource for the organization

of samples from a CPE and it is in accordance with the recommended by

the NBR/ISO/IEC 17025/05.



15

CODE 17 NANOCOSMETICS IN PHOTOPROTECTION: DEVELOPMENT AND

CHARACTERIZATION OF NANOPARTICLES WITH SUNSCREENS Nascimento, D. F.1; Silva, A. C.; Freitas, Z. M. F.1; Ricci-Junior, E.1; Santos, E. P. 1

1 Departamento de Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro. Corresponding author: [email protected] Introduction: As cancer has increased in the last decades, photoprotection has been largely recommended as a preventive measure against the harmful results of the ultraviolet radiation on skin. However, some studies show toxicity associated with sunscreen actives substance, and the most serious effect is the formation of free radicals. The sunscreens combined with nanotechnology are interesting because they decrease the systemic absorption, by improving retention on the most superficial skin layers. Objective: The purpose of this work was to develop and characterize poly-ε-caprolactone nanoparticles (Np) with octilmethoxycinnamate, octocrylene and benzophenone-3. Materials and Methods: Np were prepared by emulsion/evaporation method. Available characterization techniques were used for the evaluation of morphology (TEM), size (DLS), Zeta potencial (ZP) and encapsulation efficiency (EE%). Results and Discussion: All Np stood at the nanometric scale changing from 214.5 to 265.6 nm, with Polidispersity Index (PI) from 0.058 to 0.161, with predominantly elliptical and spherical morphologies. The ZP was slightly negative and changed from -6.05 to -11.01. The values of EE% changed from 84 to 90%. The content of the actives substances changed from 44 to 65µg/mg of Np. The process yield changed from 60 to 76%. Conclusion: The ZP values obtained suggest good stability of the system, because it hinders the flocculation. The low PI valuesdemonstrated the quality of distribution size with a good correlation between the two techniques (TEM and DLS). The values obtained for yield, EE% and content were satisfactory, suggesting the applicability of the method for developing nanocosmetics.



16

CODE 20 ANTIMYCOBACTERIAL ACTIVITY OF THE FRACTIONS AND

COMPOUNDS FROM Scutia buxifolia REISSEK. Athayde, M. L.1; Boligon, A. A.1; Cruz, R. C.1; Janovik, V.1; Agertt, V.2; Campos, M. M. A.2; Guillaume, D.3 1Phytochemical Research Laboratory at Department of Industrial Pharmacy, Federal University of Santa Maria (UFSM). 2Mycobacterial Laboratory Research at Department of Clinical and Toxicological Analysis, Federal University of Santa Maria (UFSM). 3Department of Medicinal Chemistry, Université Reims-Champagne Ardenne, 51 Rue Cognacq Jay, Reims, France. Corresponding author: [email protected] Introduction: The use of medicinal plants for the treatment of diseases, including fungal and bacterial infections is typical in Brazil and worldwide research groups screen plant extracts to detect secondary metabolites with antimicrobial properties in an attempt to find new promising compounds. Material and Methods: The antimycobacterial activity of Scutia buxifolia (Rhamnaceae) leaves extracts and fractions were evaluated for the first time against Mycobacterium smegmatis, M. tuberculosis, and M. avium using the broth microdilution method. The flavonols quercetin and quercitrin and the phenolics acids gallic and caffeic, were isolated and further quantified by HPLC-DAD. Results and Discussion: Promising anti-Mycobacterium smegmatis activity was observed with ethyl acetate extract (MIC = 312.50 μg/mL), wh ch w s f c on ed n o s c ge column chromatography and their fractions presented MIC values ranging f om 78.12 o bove 312.50 μg/mL. The f c ons III nd VI of S. buxifolia leaves showed a high level of activity against M. smegmatis (MIC= 78.12 nd 156.25 μg/mL, espec ve y), M. tuberculosis (MIC = 156.25 μg/mL) and M. avium (MIC = 312.50 μg/mL), whe e s o he o he f c ons he v ues v ed f om 312.50 o 1250.00 μg/mL g ns hese s ns. Quercetin and quercitrin were predominantly present in fractions III and VI. Flavonoids are well known for their antimicrobial potencies, as they can inhibit enzymes involved in the fatty acid and mycolic acid biosynthesis in vivo. Mycolic acids are one of the most distinctive features of the mycobacteria cell wall, essential for its survival. Conclusion: The results provided evidence that the studied plants fractions might be potential sources of new antimicrobial drug. Financial support from CNPq and CAPES, Brazil



17

CODE 21.1 DIRECT INHIBITORY ACTION OF SULFATED POLYSACCHARIDES

FROM RED SEAWEED Hypnea musciformis ON THROMBIN ENZYMATIC ACTIVITY EVALUATED BY CHROMOGENIC SUBSTRATE ASSAY Alves, M. G. C. F1.; Dore, C. M. P. G1.; Pinheiro,T. S1.; Monteiro, N. K. V1.; Costa, T. G1.; Leite, E. L1. 1 Laboratório de Glicobiologia, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN). Introduction: Thrombosis is related with cardiovascular diseases one of

the most common in the world and for its treatment is used heparin, a

sulfated polysaccharide used as anticoagulant agent but was observed

some side effects and possible contamination by prions and viruses.

Materials and Methods: In this study, we extracted a sulfated

polysaccharides, fraction 1.0 (F1.0) from red seaweed Hypnea musciformis

and assessment anticoagulant effect by activated parcial thromboplastin

time (aPTT) and prothrombin time (PT) by commercial kits. We also studied

the effect of this polysaccharydes on chromogenic substrate assay of

thrombin and FXa enzymatic activity. Results and Discussion: The

results demonstrated that this fraction was mainly composed of

carbohydrates (49.70 ± 0.10%), with high sulfate content (44.59±0.015%)

and low protein contamination (0.92±0.001%) and low phenolic compounds

(4.79±0.016%). Results obtained by aPTT displays an anticoagulant action

at different concentrations of F1.0 (p<0.001) showing an increasing in the

clot formation time at 25, 50, 100, 150, 200, 250, 350 µg. F1.0 showed in all

tested concentrations small clot formation time by PT when compared with

negative control (p<0.05). Using the chromogenic substrate assay of

thrombin and FXa were observed that F1.0 in tested concentrations (150,

250, 350 µg) displayed moderated inhibitory action on enzymatic activity of

thrombin (40.00 ± 2.26%, 50.67 ± 2.64% and 45.73 ± 6.60%) and

stimulatory action on FXa (8.51%, 4.93% and 9.70%), respectively.

Conclusion: We conclude that this sulfated polysaccharide (F1.0) showed

low anticoagulant activity determined by aPTT with direct inhibitory action of

thrombin.

Financial support from CNPq/Brazil



18

CODE 21.2 INHIBITORY ACTION ON LIPID PEROXIDATION AND PROTECTIVE

ACTIVITY ON H2O2-INDUCED HEMOLYSIS OF SULFATED POLYSACCHARIDES F1.0 FROM RED SEAWEED Hypnea musciformis Alves, M. G. C. F1.; Dore, C. M. P. G1.; Medeiros, H. N1.; Paiva A. A. O1., Filgueira, L. G. A1., Rocha, H. A. O1., Benevides, N. M. B2. Leite, E. L1. 1 Laboratório de Glicobiologia, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN). 2 Universidade Federal do Ceará (UFC).

Introduction: The normal metabolic process promotes reactive oxygen species (ROS) generation, which can induce several pathologic effects. Antioxidant agents slows or prevents oxidizable cellular substrate oxidation leading to ROS generation inhibition or scavenging. In their natural habitat, marine seaweed are exposed to high oxygen concentrations and light, promoting free radical generation, but they do not suffer any photodynamic damage, which suggests the presence of antioxidant components. Objective: In this study was evaluated in vitro antioxidant activity of sulfated polysaccharides F1.0 from red seaweed Hypnea musciformis. Results and Discussion: F1.0 was mainly composed of carbohydrates (49.70 ± 0.10%), with high sulfate content (44.59±0.015%) and low protein contamination (0.92±0.001%) and low phenolic compounds (4.79±0.016%). In the lipid peroxidation system induced in egg yolk sulfated polysaccharides F1.0 promotes the inhibition around 57.92% at 0.08 mg/mL, showing the high inhibition power of F1.0 with IC50 of 0.0030 mg/mL. Sulfated polysaccharides F1.0 at 5 mg/mL promotes 67.89% hemolysis inhibition with an IC50 of 0.3750 mg/mL in H2O2-induced hemolysis model. These polysaccharides F1.0 showed no cytotoxic activity on erythrocytes in direct hemolysis model, demonstrated by the non-occurence of significative hemolysis in all tested concentrations. No statistically significant difference was seen between erythrocytes of the Rh positive and negative groups or among the erythrocytes of the B, AB, and O groups. Conclusion: Besides the protective action on H2O2-induced hemolysis, sulfated polysaccharides F1.0 does not cause significant damage action on erythrocyte membranes of different ABO and Rh blood groups and showed strong inhibitory action on lipid peroxidation assay. Financial support from CNPq/Brazil



19

CODE 23 DETERMINATION OF ACIDITY IN CURD CHEESE OF CAICÓ AND

JUCURUTU Amaral, N. K. M.1; Teixeira, L. L.1; Silva, A. P. O1.; Sousa, P. R. F.1.; Carvalho, B. G.1; Moura, M. F. V.1 1Programa de Pós – graduação em Química – PPGQ Introduction: Cheese is a concentrated milk consists of proteins, lipids,

carbohydrates, minerals, calcium, phosphorus and vitamins. The

classification of cheese based on characteristics due to the type of milk

used, the type of coagulation, the consistency of pulp, fat, the type of shell,

healing time, etc. Curd cheese is a dairy product widely produced in the

Northeast region of Brazil. Objective: This work stood the determination of

acidity in the cheese sample of cities Caico and Jucurutu in Rio Grande do

Norte. Material and Methods: We followed the methodology

recommended by the Institute Adolfo Lutz (2008) for determination of

acidity in lactic acid. Results and Discussion: We observed a lactate

curve with four points for each sample, which initially Jucurutu cheese had

the lowest levels, but during the 60 days the positions were reversed.

Comparing the results obtained by Leopoldina (2008) who analyzed

cheese curd Currais Novos presenting results of 0.27 g lactic acid per 100g

of cheese, one can see that the samples analyzed in this study showed

acidity of lactic acid lower that Leopoldina (2008).



20

CODE 24 Toxicologic assay of ethanolic extract from Xylopia cayennensis

Silva, A. D. V.¹; Pontes, A. C. A. de A.¹; Campos, D. M.¹; Da Silva, M. L.¹; Nascimento, D.; Martins, M.; Dolabela, M. F.² 1Discente do Curso de Graduação em Farmácia da Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará (UFPA). 2Docente da Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará (UFPA). Corresponding author: [email protected] Introduction: Xylopia cayannensis is popularly known as "embira." This plant is rich in reducing sugars, tannins, catechins, alkaloids, steroids and triterpenoids, depsides and depsidones. Objective: This study aimed to evaluate the toxicity from the ethanolic extract of branches and leaves of Xylopia cayannensis (EEX). Materials and Methods: The lethality test of EEX on Artemia salina Lech. to obtain the median lethal concentrations (LC50) was performed according to Meyer et al (1982). Twenty Artemia salina larvae were transferred to test tubes containing artificial sea water and EEX in six different concentrations. The tests were done in triplicate. The counting of live and dead larvae was performed after 24 hours. Results and Discussion: The lowest concentration (0, 46875 mg/mL) had confirmed mortality larvae between 35% (7) and 40% (8). The intermediate concentrations (1.875 mg/mL and 3.75 mg/mL) caused mortality of 75% (15) and 90% (18) respectively. The highest concentrations (7.5 mg/mL and 15 mg/mL) had of lethality 100% (20). The LC50 was 0, 9375 mg/mL, less than 1mg/mL, indicating that EEX presents a high level of toxicity when compared to the study of the species Xylopia emarginata, in which was verified LC50 of 0.127 mg/mL. Conclusions: The methodology with Artemia salina Lech. was a simple and inexpensive test that provides results in a short time. However, for a more specific evaluation from the toxicological activities of EEX, cell-culture toxicological bioassays must be done. Financial support from CNPq/Brazil and SESu/MEC/Brazil.



21

CODE 25 ANTIMICROBIAL ACTIVITY EVALUATION OF COPAIBA OIL

MICROEMULSION SYSTEM: A PRELIMINARY STUDY Alencar, E. N.1,4; Xavier - Júnior, F. H.2,4; Morais, A. R. V.3,4; Silva, K. G. H. 3,4; Paula, P. R.4; Egito, E. S. T.1,2,3,4 1Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde (CCS), Universidade Federal do Rio Grande do Norte (UFRN), Natal – RN. 2Programa de Pós-graduação em Biotecnologia (Renorbio), UFRN. 3Programa de Pós-graduação em Ciências da Saúde, CCS, UFRN. 4Laboratório de Sistemas Dispersos (LaSiD), Departamento de Farmácia, CCS, UFRN. Corresponding author: [email protected] Introduction: Copaiba oil is used as a popular anti-inflamatory and anti-infective agent to treat several diseases. However, it is necessary to use a system that makes its use more pleasant and efficient, such as the microemulsions. These systems are colloidal dispersions thermodynamically stable of water and oil stabilized by a surfactant. Objective: The aim of this work was to perform a preliminary antimicrobial activity study of Copaiba oil free and loaded into microemulsions against bacterial and yeasts strains. Material and Methods: The screening test by modified agar diffusion was performed for all strains and the minimum inhibitory concentration (MIC) was evaluated by agar dilution, for bacteria (Staphylococcus aureus, Enterococcus faecalis, Salmonella Typhimurium, Pseudomonas aeruginosa), and broth microdilution, for yeasts (Candida albicans, Candida krusei and Cryptococcuss neoformans). Results and Discussion: The copaiba oil had activity against the yeasts, however the microemulsions were unable to inhibit the fungal growth. These systems had a lower MIC value to inhibit Staphylococcus growth lower than the oil. For example, the MIC of copaiba oil was 1mg/mL and the microemulsion value was lower than 0.25mg/mL for the same clinical strain of S. aureus. In short, it can be suggested that microemulsions were able to increase the Copaiba oil activity against bacterial strains. Also it can be speculated that these drug delivery system can modify the release and delivery of the oil compounds for specifics targets. Thus, further study is important in order to confirm the results on the antimicrobial activity of copaiba oil microemulsions, which can became a therapeutic choice to treat several diseases. Financial support from CNPq and CAPES/Brazil.




22

CODE 26 PROFILE AND QUALITY OF MEDICATIONS PRESCRIPTION IN THE

CITY OF CUITÉ – PB, BRAZIL. Oliveira, J. R. M. de1; Souza, F. B. de; A. D.1; Martins, R. R.1

1 Federal University of Campina Grande - UFCG, Health and Education Center – CES, Cuité-PB, Brazil. Corresponding author: [email protected]. Introduction: The prescription has a direct action on qualitative and quantitative aspects of drug use. It is essential that accurate and legible to avoid errors during interpretation by the patient or health professionals. Prescription medications must contain the appropriate, correct amount and proper dosage. Objective: to assess the quality and profile of drug prescription in Cuité – PB, Brazil. Material and methods: the random sample was made up from 10% of prescriptions issued in the first half of 2010 (1772 prescriptions). The prescribed drugs were categorized according to the ATC. Results and Discussion: drugs used to treat hypertension, analgesics and antipyretics, antidiabetics, antibiotics and anti-inflammatory drug classes correspond frequently. The average number of drugs per prescription was 1.7. The parameters were: 44.8% of prescriptions had no concentration, 21.5% lack of pharmaceutical form, without the amount 8.8%, 14.8% non-dosage, 2.5% without the duration of treatment and 2 5% without the prescriber's signature. Conclusion: Overall, the data show serious deficiencies in the writing of prescriptions. It might be noted that the most representative classes of medications are drugs for treatment of chronic diseases, antibiotics, analgesics and anti-inflammatory.




23

CODE 27.1 INVESTIGAÇÃO DAS CLASSES METABÓLICAS DA ESPÉCIE

BOTÂNICA Xylopia cayennensis Da Silva, M. L.¹; Silva, A. D. V.¹; Pontes, A. C. A. A.¹; Campos D. M.¹; Nascimento, D.; Dolabela, M. F.² 1Discente do Curso de Graduação em Farmácia da Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará. 2Docente da Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará. Corresponding author: [email protected] Introduction: The botanic species Xylopia cayennensis (XC) is a member from the family Annonaceae, popularly known as "embira" and is used by traditional populations for its cardiovascular effects. Objective: This work aimed to carry out phytochemical approach to the identification of XC metabolic classes. Materials and Methods: The XC aerial parts (stems and leaves) were subjected to grinding process to powder obtaining. To obtain the ethanolic extract (EE), 200g of the powder were measured with a precision scale and taken to the percolator, which were added 2L of ethylic alcohol (ratio 1/10). After seven days the EE was removed from the percolator and filtered. The filtrate was taken to the rotary evaporator to be concentrated. Then, the EE was placed in a becker, weighed and left in an heater at 40 ° C during one week to complete solvent drying and obtaining of the dry extract (DE). The phytochemical tests were carried out to identify metabolic classes using the methodology previously described by Barbosa et al. (2004). Results and Discussion: From all the tests performed during phytochemical approach, six showed positive results, indicating the presence of the following metabolic classes: reducing sugars, tannins, catechins, alkaloids, steroids and triterpenoids, depsides and depsidones. Some of these classes were previously described in other studies. Conclusions: There are few studies of the pharmacological activities with XC, so this first approach was important because it will serve as guidance for identification tests of XC pharmacological activities. Financial support from CNPq/Brazil and SESu/MEC/Brazil.



24

CODE 27.2 Phytochemical and chromatographic analysis of Mikania glomerata

Da Silva, M. L.¹; Malcher, N. S.¹; Nascimento, M. S.²; Nascimento, J.²; Barbosa, W. L. R.³ 1Discente do Curso de Graduação em Farmácia da Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará (UFPA). 2Discente do Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará (UFPA). 3Docente da Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará (UFPA). Corresponding author: [email protected] Introduction: The species Mikania glomerata Sprengel and Mikania laevigata Schultz Bip are popularly known as "Guaco". However, Mikania glomerata has been more used as a medicinal plant. These species are primarily intended for the respiratory diseases treatment. Although, other pharmacological properties of Mikania glomerata were already reported, as anti-inflammatory, analgesic, antioxidant, which are associated with the presence of flavonoids, tannins, saponinas and coumarins (ROCHA et al., 2008). Objective: Expand the scientific knowledge about Mikania glomerata, showing the phytochemical approach and chromatographic character of the extract. Materials and Methods: From the botanical material tincture were performed phytochemical tests, fractionation of solid-liquid partition, spectrophotometric analysis and thin layer chromatography (TLC). Results and Discussion: The phytochemical approach had positive results for: reducing sugars, phenols and tannins, alkaloids, and depsides depsidones, and coumarin derivatives. In the analysis by TLC, the bands formation was observed with the use of the eluents hexane/acetone and toluene/ dichloromethane/acetone, with the aid of ultraviolet light at 365 nm and 254 nm; and after revelation through specific reagents as vanillin and potassium hydroxide (5%). On the spectrophotometric analysis it was observed that there is a greater light absorbance when the wavelength is between 300 to 400 nm. Similar findings were previously observed (BOLINA et al., 2009). Conclusions: It is possible to conclude that the results corroborate other studies previously reported in the literature and that the species M. glomerata has been a great research target due to their pharmacological potential. Financial support from CNPq/Brazil and SESu/MEC/Brazil.



25

CODE 28 ZINC OXIDE EMULSION: A NEW ALTERNATIVE TO DIAPER

DERMATITIS TREATMENT Santiago, R. R.1,2; Ferreira, M. R. A.1; Silva, K. G. H.2; Silva, K. C. H.2, Svidizinsk, T. I. E.3; Egito, E. S. T.1,2

1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte (UFRN). 2Laboratório de Sistemas Dispersos, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 3Laboratório de Micologia Clínica, Departamento de Análises Clínicas, Universidade Estadual de Maringá (UEM). Corresponding author: [email protected] Introduction: Diaper dermatitis, popularly known as diaper rash, is an inflammation of the skin covered by the diaper, affecting 50% of children. Treatment usually involves increasing the frequency at diaper changing, use of super-absorbent disposable diapers and topical agents such as creams or barrier creams such as those using zinc oxide (ZnO). Thus, appears as a new perspective for treatment of diaper dermatitis the incorporation of zinc oxide in emulsified vehicles where the component of lipophilic phase had a synergistic effect with the active drug. Objective: The aim of this work was to develop and to characterize emulsion systems with sesame oil as lipophilic phase added of zinc oxide. Materials and Methods: Therefore, pseudo-phase ternary diagrams were used as tool for identification of viscous emulsion region. Results and Discussion: The dispersed systems thus obtained were macroscopically classified, and only seven viscous emulsion formulations were selected for further studies. The stability of all formulations was analyzed by macroscopic analysis, stability under storage, stability under centrifugation, freeze/thaw cycles, pH evaluation, conductivity measurements, particle size analysis and rheological behavior. After performing the stability studies, the formulation which was more stable was selected for the addition of ZnO. Conclusions: Moreover, this formulation showed good results when tested against reference bacteria and yeasts, demonstrating that it has considerable antimicrobial activity, particularly against Candida albicans, the microorganism commonly associated with diaper dermatitis. Financial support from master scholarship from CAPES/Brazil



26

CODE 30 LEGAL ANALYSIS OF MEDICINES ADVERTISING TRANSMITTED IN

RADIO STATION FROM NATAL/RN ACCORDING TO RDC N° 96/08 Batista, A. M.1; Carvalho, M. C. R. D.2

1Professora Assistente I do Departamento de Farmácia da Universidade Federal do Rio Grande do Norte (UFRN). Departamento de Farmácia da Universidade Federal do Rio Grande do Norte (UFRN). Laboratório de Monitoração de Propaganda de Produtos Sujeitos à Vigilância Sanitária, Natal/RN. 2Professora Associada I do Departamento de Farmácia da Universidade Federal do Rio Grande do Norte (UFRN). Laboratório de Monitoração de Propaganda de Produtos Sujeitos à Vigilância Sanitária. Natal/RN. Corresponding author: [email protected] Introduction: Medicines advertising is one of the most important marketing resources used by the pharmaceutical industry to induce people to buy these products although they do not have the real necessity to use them. Objective: To evaluate drugs advertisings transmitted on radio stations in Natal/RN, from April 2010 to September 2010. Materials and methods: The capitation of advertising pieces was accomplished through recording the transmitted programs in 9 radio stations, during 2 hours transcribed integrally. For that, was elaborated a schedule of transmitted programs in each station with their respective timetable, in way to include the 6:00h to 18:00h period. Legal analysis was based on RDC n° 96/08 from ANVISA, specific for propaganda control of medicines. Results: The analysis of 06 captured advertising pieces demonstrates that none of them accomplishes in full legislation demands; 100% omit active principle; 83,3% omit contraindications; 16,7% announce no registrated medicines in ANVISA; 16,7% omit indications; 16,7% suggest or stimulate diagnosis. Conclusions: The study indicates the necessity of the topic drugs advertisements to be treated in a wide context, that is, to be discussed as a public health concern. Although the advertising regulation and inspection is the State responsibility, this should be shared with the advertising agency, pharmaceutical industry and media. Furthermore, it is indispensable to inform and to conscience the population of their rights in such mistaken situation.



27

CODE 31.1 STUDY OF ISONIAZID ADSORPTION ONTO CHITOSAN: ASSAYS OF

EQUILIBRIUM AND KINETICS USING STIRRED TANK Jácome, R. C.1; Gonçalves, L.R.B 2; Adriano, W. S.1*

1Laboratório de Ciências Farmacêuticas, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG). 2Laboratório de Processos Biotecnológicos, Departamento de Engenharia Química, Universidade Federal do Ceará (UFC). Corresponding author: [email protected] Introduction. Tuberculosis is a health problem in Brazil and other twenty one countries, which, together sum 80% of all cases in the world. For the treatment of this disease, isoniazid remains the drug of choice. Adsorption has been used as one of the promising techniques seeking low-cost adsorbents with high adsorption capacity. Objective. Thus, objective was to investigate isoniazid adsorption on chitosan promoting an alternative using of regional products as adsorbents and bringing potential social-economic benefits by developing an innovative technology. Material and Methods. Maximum adsorption capacity isotherm was determined from tests in stirred tank trough Langmuir`s isotherm, using isoniazid prepared in 20 mM sodium acetate buffer, pH 4.0 at different concentrations, with constant mass of adsorbent during one hour, being carried out kinectics assays and desorption tests. Results and Discussion. Results showed that Langmuir isotherm model fitted to experimental datum with maximum adsorption capacity of 55.2mg of isoniazid per gram of adsorbent and dissociation constant of 0.20mg.mL-1 being kinetic process about 10 min which facilitates process of drug separation. Previous desorption assays showed isoniazid releasing under high ionic strength condition (NaCl 2M). Conclusions. Use of chitosan is feasible for the isoniazid separation process by adsorption, as their preparations are simple, presenting low cost combined with high adsorption capacity. Financial support from CNPq/Brazil.



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CODE 31.2 HOUSEHOLD SURVEY ABOUT THE PROFILE OF DRUG USE BY

ELDERLY IN THE CITY OF CUITÉ-PB. Jácome, R. C.1; Almeida, M. A. A. L. S.1; Batista, M. T.1; Martins, R. R.2; Nagashima Jr, T.2

1Discentes do Curso de Bacharelado em Farmácia, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG), Cuité-PB. 2Docentes do Curso de Bacharelado em Farmácia, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG), Cuité-PB. Corresponding author: [email protected] Introduction. The population aging is a global phenomenon that has repercussions on changing habits and behaviors. In this context the elderly need new assistive technology for the maintenance of health care and for these cases, drugs are being widely used Objective. The aim of this work was to evaluate the profile of drug use by elderly people residing in Cuité-PB. Material and Methods. An observational and descriptive study carried out in 60 households randomly selected. Data collection took place through individual structured interviews realized in april/2010 and data analysis used the software excel 2007. Results and Discussion. The sample was composed of 54 interviewed aged between 60 - 95 years. Results shows that there was an average of 3.7 person by house living of which 27.5% of them were elderly. Was observed among the elderly, low income and low educational level, these factors may be related to inappropriate use of medicines. The classes of medications most prevalent by elderly people were the anti-hypertensive (34.8%), analgesics and anti-inflammatory drugs (23.2%) and hypoglycemic (12.5%). Conclusions. This work demonstrates that the chronic use of drugs is associated with a low income and low educational levels. This information is essential for adequate planning of health care, contributing to the rational use of medicines.




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CODE 32 ORAL ADMINISTRATION OF EXTRACT CYANOBACTERIAL BLOOM

IN PREGNANT RATS: A REPRODUCTION ASSESSMENT Almeida, C. S.1; Arruda, A. C. C.1; Barbosa, P. F.1; Dantas, D. H. M.1; Costa, H. T. M. L.1; Costa, I. A. S. O.2; Lemos, T. M. A. M.1; Schwarz, A.1; Pinto, E.3; Kujbida, P.1

1Laboratório de Toxicologia, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN). 2Laboratório de Ecologia e Toxicologia de Microrganismos Aquáticos, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte UFRN). 3Laboratório de Toxinas e Produtos Naturais de Algas, Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo (USP). Corresponding author: [email protected] Introduction: Lots of researches have been focused on the pure cyanobacterial toxins, but little on the natural cyanobacterial bloom. Objective: This study was undertaken to investigate the effect of the natural cyanobacterial bloom extract by oral via (gavage) on the female reproductive performance. Materials and Methods: Hydrophilic Interaction Chromatography/ fluorescence Jetection and Liquid Chromatography/tandem mass spectrometry toxin analyses were performed on surface water samples from 37 reservoirs in the Rio Grande do Norte and some microcystins (MCs) congeners were detectable in 14 samples. Sum of all variants the values of MCs varied from 0,22 to 51,86 ng/mL. It was used the most concentrated sample to exposure adults females Wistar rats. The extract of cyanobacteria bloom was administered at first gestational day (GD) to 4 different groups (10/group) in different doses (0, 250, 100 and 40 µg MCs/ Kg body weight/day. At GD 20 the rats were euthanized for biochemical and reproductive studies. Results and Discussion: The statistical analysis showed absence of alterations at body weight, body weight gain, food and water intakes, organ weight/body weight ratios and at AST, ALT, creatinine and urea serum levels in both studies. The reproductive performance (the number of implanted embryos, pre-implantation loss, resorptions, corpora luteo, implantation, live and dead offspring) was similar between control and expepemental dams. Conclusions: These findings suggest that the toxic cyanobacterial bloom administered at this concentration of MCs during gestation is not able to promote maternal toxicity and to impair gestation or reproductive performance of rats. Financial support from CNPq/Brazil



30

CODE 33 COMPARATIVE ANLYSIS OF PHYSICAL AND CHEMICAL

PROPERTIES OF TWO SOURCES OF WATER FOR USE ON SCHOLL PHARMACY MANUEL CASADO ALMEIDA, CUITÉ-PB Lima, D. D. F. S1; Lima, W. S.1; Alencar, J. S.1; Silva, P. S. G.1; Almeida, M. A. A. L. S.1

1 Universidade Federal de Campina Grande / Centro de Educação e Saúde, Unidade Acadêmica de Saúde, O ho D’águ d B c , s/ n, Cuité, PB, Brasil 58175-000. Corresponding author: [email protected] Introduction: According to the RDC 67/07, the water used in the manipulation must be obtained from drinking water, treated in a system that ensures the attainment of water with pharmacopoeial specifications for purified water. Objective: The study aimed to the physical and chemical analysis of two different types of water by comparing them with a benchmark to determine the font to be used for treatment and use in the scholl pharmacy Manuel Casado Almeida. Materials and Methods: Analysis were performed with natural spring water and tap water, and deionized water as standard. The methodology used was to measure pH, turbidity, conductivity, content total solids and content chloride. Results and Discussion: The analysis showed values of pH approximate between tap water and deionized water, and 6,73 and 6,90, different from the natural spring that was 3,89. In the determination of conductivity, total solids and chlorides, the natural source and tap water obtained similar values, according to the following values: 2.65 and 2.72 mS /cm 0.20g and 0.18 g and 40.64 g/100 ml and 40 , 07 g/100 ml respectively. The turbidity of the 2.25 NTU natural source was lower than 5.86 NTU tap water. Conclusions: The results have properties compatible between the two waters, differing in the aspects of pH and turbidity, confirming the economic viability of using natural source for the supply. Corrections of the parameters of chosen water can occur through interventions in the process of purification that meets the quality specifications required by law



31

CODE 34.1 QUALITATIVE TESTS OF AMPICILLIN SODIUM POWDER FOR

INJECTION Tótoli, E. G.1; Salgado, H. R. N.1 1Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP) Corresponding author: [email protected] Introduction: Amp c n sod um s β-lactam antibiotic for parenteral use, belonging to the penicillin group. Ampicillin was the first semi-synthetic penicillin that showed activity against Gram-negative bacteria, because of its greater ability to penetrate the outer membrane of these micro-organisms, opening the field of broad-spectrum penicillins. Objective: This study aims to develop qualitative tests for ampicillin sodium injectable. Materials and Methods: The tests performed with ampicillin sodium powder for injection were as follows: solubility (in solvents with different polarities), determination of average weight, moisture content determination (by an infrared moisture balance), pH determination and thin-layer chromatography (TLC) (mobile-phase: ethanol : water, 80:20 / stationary phase: silica). Results and Discussion: It is easily soluble in water, acetic acid 0.1 M, sodium hydroxide 0.1 M and hydrochloric acid 0.1M. In addition, it is soluble in methanol and slightly soluble in ethanol. However, it is insoluble in dichloromethane, acetonitrile, ethyl ether, chloroform, acetone, ethyl acetate and butanol. The average weight of the vials is in accordance to specifications. It has 2.87% ± 0.04% of moisture. The determination of pH showed that ampicillin sodium injectable is basic, with pH of 8.72. The Rf value for the TLC is 0.84. Conclusion: The tests performed are excellent to identify ampicillin sodium powder for injection. Qualitative methods are essential because they allow determining if the product is in accordance to specifications and they also enable the realization of predictions for quantitative methods. Finalcial support: FAPESP, CNPq, PADC-FCF and União Química – Pharmaceutical Industry.




32

CODE 34.2 DEVELOPMENT AND VALIDATION OF TURBIDIMETRIC ASSAY TO

POTENCY EVALUATION OF AMPICILLIN SODIUM FOR INJECTION Tótoli, E. G.1; Salgado, H. R. N.1 1Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP) Corresponding author: [email protected] Introduction: Ampicillin sodium is a semi-synthetic β-lactam antibiotic for parenteral use, belonging to the penicillin group. Microbiological assays are described for several antimicrobial agents, because they make possible to check the potency of the product against a microorganism. Objective: The objective of this study was to develop and validate simple, sensitive, a precise and accurate microbiological assay by turbidimetry, to quantify ampicillin sodium injectable. Materials and Methods: In six test tubes con n ng 10 mL of TSB b o h, w s dded 600 μL of a Staphylococcus aureus 25923 suspension, standardized to 25% transmittance in spec opho ome e (580 nm). In h ee of hese ubes w s dded 200 μL of s nd d so u on nd n he o he h ee, 200 μL of s mp e so u on he same concentrations of stand d so u ons (2.0; 4.0 nd 8.0 μg/mL). I w s performed in triplicate. The test-tubes were incubated in a Shaker, in water bath, for 4 hours, and then the absorbances were checked in a spectrophotometer (530 nm). A curve of absorbance by the log of concentration was constructed. Results and Discussion: The potency verified was 97.82%. The method showed linearity, with correlation coefficients of 0.9999 (standard) and 0.9998 (sample); accuracy (mean recuperation = 100.76%); precision inter-assay (R.S.D. = 0.76%) and robustness. Conclusion: The potency method was validated and showed to be adequate for determining the of ampicillin sodium injectable. In addition, it is fast, easy, economical, and can be used in routine analysis of Quality Control. Finalcial support: FAPESP, CNPq, PADC-FCF and União Química – Pharmaceutical Industry.




33

CODE 35 TECHNICAL AND ADMINISTRATIVE MANAGEMENT OF SCHOOL

PHARMACY'S WAREHOUSE MANOEL CASADO DE ALMEIDA – UFCG/CES. Medeiros J. R. I. J.1; Lima, W. S.; Alencar, J. S. Farmácia Escola Manoel Casado de Almeida, Curso de Bacharelado em Farmácia, Universidade Federal de Campina Grande/Centro de Educação e S úde, O ho D’águ d B c , s/n, Cu é, PB, B s . Corresponding author:[email protected] Introduction: The School Pharmacy, besides acting as an academic support, should, in fact, have returns for their maintenance and competitiveness with other establishments present in the town of Cuité – PB. To do so, ’s necess y o eso o m n gemen oo s n o de o reduce production costs and obtain products with good quality. One way to achieve this is the stock management related to the implementation of the Good Manufacturing Practice s in Pharmacy (GMPs). Objective: Given this fact, this study aimed to plan and organize the warehouse of the School Pharmacy Manoel Casado de Almeida UFCG/CES. Material and Methods: Following the Resolution No. 67, October 8, 2007 ANVISA – which establishes the GMPs in pharmacies, the layout of the school pharmacy was designed within the required standards and Standard Operating Procedures (SOPs) of the cleaning and material inflow and outflow. Results and Discussion: The layout of the school pharmacy is a key to the development of GMPs, once that a physical structure facilitates proper working conditions and avoids storage of pharmaceutical input. SOPs designed to ensure integrity of the received and stored material, which along with the stock quantitative management (curve ABC) can m n m ze he o c p nves ed n s ock. I ’s expec ed h w h he implementation of management measures, the costs may be reduced and that the material received may be in ideal conditions for use, thus providing more competitiveness in pharmacies compared to other pharmacy outlets.




34

CODE 36 VALIDATION OF A SPECTROPHOTOMETRIC METHOD (UV-VIS) FOR

DETERMINATION OF TOTAL FLAVONOIDS OF THE RED PROPOLIS FROM ALAGOAS: USE OF CATECHIN AS BIOMARKER Oliveira, J. M. S.1; Silva, L. R.1; Neto, J. C1.; Arruda, R. E. S.1; Nascimento, T. G.1; Junior, I. D. B.1

1Laboratório de Análises e Controle de qualidade de Fármacos e Medicamentos, Grupo de Pesquisa em Tecnologia e Controle de Qualidade de Medicamentos,Curso de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas (ESENFAR/UFAL) Maceió, Alagoas. Corresponding author: [email protected] Introduction: The red propolis obtained from mangroves region of Alagoas presents organoleptic, chemical and biological characteristics, which are different from other types of propolis from Brazil. Currently, the phytotherapy industries have necessity to develop low cost methods and quick for quality control assays of propolis. OBJECTIVE: The aimed of this work was to develop and validate a spectrophotometric method (UV-vis) for determination of total flavonoids of red propolis. Material and Methods: Catechin was purchased from Sigma-Aldrich (98%). Propolis (in nature) was collected in the region of mangroves in Marechal Deodoro city. Tincture of propolis (20%) was obtained after extraction by maceration and concentration in rotaevaporador. Catechin standard solution (2000μg/mL) nd nc u e of p opo s (2000μg/mL) we e p ep ed n e h no nd d u ed o concen on nge of 8.5 o 85μg/mL nd 5.0 o 70 mg / mL, respectively, for purpose of establish parameters of validation described in the RDC 899/2003 of ANVISA. Results and Discussion: The spectrophotometric profile of tincture and sub-fractions of red propolis was similar to catechin with maximum wavelengths of 212 and 280nm. The method proved to be easy turn and quick execution, besides to present excellent selectivity and adequate limit of quantification for phytotherapics and opotherapics. The method showed linearity and less variation (2.6% and 6.9%) of intermediate precision and accuracy (3.5% and 7.1%) for catechin and tincture of propolis, respectively. Conclusion: The UV method complies with the validation requirements. Catechin may be a marker for determination of total flavonoids of red propolis. Financial support from CNPq/Brazil.



35

CODE 37.1 CONSTITUENTS STUDY OF FIXED Pike NORTHEAST Caryocar

coriaceum Wittmer. Castro, K. M.1; Rodrigues, N. R.1; Bandeira, M. A. M.1 1Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Ceará (UFC). Corresponding author: [email protected] Introduction: Piquizeiro tree size is a robust Caryocar belonging to the genus with about 20 different species. In Brazil, the species occurs on

Wittmer Caryocar coriaceum Cariri CE . In addition to its importance in food, is widespread in folk medicine the use of regional peak oil added to the honey against colds and bronchitis. In our previous work we determined the major fatty acids that make up this oil with their respective levels (palmitic, oleic acid and 27.87%, 65.71%) and also the presence of carotenoids.Objective: To study the constituents of the fruits fixed in order to determine bioactive chemical constituents. Methodology: The ripe fruits were collected in the mountains of Araripe, Cariri_CE region. For the work of isolating the chemical constituents fixed prepared the ethyl acetate extract through cold maceration epicarp and mesocarp.Evaporated at room temperature. Purified extract was obtained by chromatographic column of silica gel using as eluent dichloromethane and acetone in increasing polarity.The obtained fractions were analyzed by thin layer chromatography, collected and coded. Results: The fraction F (10-13) presented in the form of white solid, which was subsequently purified on Sephadex using methanol as eluente.Houve prominently in fractions that contained a white crystalline solid, coded as F (7). Conclusion: The spectra of RMN1H, RMN13C, DEPT, HMQC and HMBC of this substance show that it was a triterpene.



36

CODE 37.2 GUIDANCE ON PHARMACEUTICAL PREPARATIONS MEDICINAL

PLANTS WITHHOMEMADE: A STRATEGY FOR FAMILY HEALTH Sousa, L. C.1; Pinto F. A.2; Castro, K.M.1; D. Marino, R. S.1; Flag, M. A. M.1 1Department of Pharmacy, Dean of Extension ( UFC) Fortaleza, Brazil. 2Prefeitura Hall of Fortaleza, Fortaleza, CE, Brazil. Corresponding author: [email protected] Introduction: Project's CEDEFAM Phytotherapy (Center for Family Development) came the need for drugs in the community do Planalto do Pici and was structured to providepharmaceutical care in the field of Herbal Medicine in Public Health. It consists of a Garden of Medicinal Plants, which consists of plants that have proven efficacy and safety, based on the Project of Living Pharmacies UFC (FJA Matos). The present work aims to develop a strategy for the health of the family through guidance onpharmaceutical preparations homemade herbal. Methods: The guidance on pharmaceutical preparations was performed using homemade leafletindicated for each medicinal plant and type of preparation. At the time of guidanceusers received free plant material fresh and joined. Results conclusions: The preparation was the most suitable home for leaking chamber. Other preparationswere also targeted: basil infusion, infusion of eucalyptus, lemongrass tea among others. Most people who received such guidance were mothers (89%). Medicinal plants for being part of the therapeutic family arouse interest and community acceptance for this type of guidance pharmaceutical checking for their scientific importance.



37

CODE 38 STUDY OF THE TOXICITY OF ESSENTIAL OIL OF Cymbopogon

citratus (DC) STAF. FOR A POSSIBLE APPLICATION AS ANTIFUNGAL DRUG Vasconcelos, L. B.1; Carmo, E. S.1,2*, Lima, I. O.2; Estevan, E. C.2; Gayoso,

C. W.3; Diniz, M. F. F. M.4; Araújo, M. S. T.4; Lima, E. O.2

1Academic Unit of Health, Center Education and Health, Federal University of Campina Grande (UFCG). 2Program of Pos-Graduation in Natural and Syntetics Bioactive Products, Pharmaceutical Technology Laboratory, Health Sciences Center, Federal University of Paraíba (UFPB). 3Department of Dermatology, Center of Health Sciences, Federal University of Paraíba (UFPB). 4Laboratory of Toxicology, Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Paraíba (UFPB). Corresponding author: [email protected] Introduction The number of bacterial and fungal infections has increased worldwide, thus the antimicrobial available in the market have become insufficient, especially by the increasing number of resistant microorganisms. In this context, researches have been conducted in order to investigate more effective and low toxicity alternative therapies, like the pre-clinical trials developed with the essential oil of Cymbopogon citratus, oil with antimicrobial activity in vitro scientifically proven. Objective This study was conducted to provide data on acute toxicity and skin irritation in animals exposed to the essential oil of Cymbopogon citratus for a possible application this as antifungal drug. Materials and Methods The acute toxicity test was carried in two groups of six mice (Mus musculus) male and female who received a dose of 2000 mg/kg (oral). To the skin irritation experiment, we used albino rabbits that received topically 0.5mL dose of 1.25 μL/mL of the same oil and were observed for 72 hours, to the presence of edema, erythema or pressure ulcers. Results and Discussion No deaths or significant differences in body weight and organs weight between control and treated animals. Mice showed no changes in general appearance, behavioral or physiological activities significant. No sign of skin irritation in rabbits was observed. Conclusions Data presented contribute to a possible safe use of the essential oil of C. citratus how antifungal in the future.




38

CODE 39.1 PRELIMINARY STUDY OF THE TOXICITY OF Aspidosperma excelsum

BENTH (APOCYNACEAE). Gomes, L. F. S.1; Dolabela, F.1; Moares,.1,Barbosa, W. L. R1,Vasconcelos, F.1 1Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará. Corresponding author: [email protected] Introduction: The genus Aspidosperma (Apocynaceae) occurs from

Mexico to Argentina and is distinguished by the frequent occurrence of indole alkaloids. Amazon is a species used by indigenous and mestizo for various medicinal purposes (anti-inflammatory, fever and cancer, among others) and several indications as potent antimalarial agent. It has been reported the toxicity of A. pyrifolium Mart in goats and has also been demonstrated in mice the toxicity of A. subincanum. and was also reported that A. pyrifolium, was able to induce abortion in rats and cause pathological changes. Objective: Evaluate the cytotoxicity of the

hidroethanolic extract of the A. execelsum Benth (HEEAs) and alkaloidal fractions.Perform the test for acute oral toxicity of the HEAs. Materials and Methods: HEEAs preparaition: 500g powder from the bark of the trunk of

A. excelsum Benth, were kept in ethanol 70º v/v for 10 days.After removal of the ethanol the hydroethanolic extract of the A. excelsum Benth (HEEAs) was lyophilized and were prepared two fractions, methanol and alkaloid. Cell culture: The effects citotoxic of the HEEAS was performed in cultured fibroblast L929 cell line. L929ce s we e m n ned n Du becco’s mod f ed E g e’s méd um supp emen ed w h 10% fe c f se um nd antibiotics (100units/ml of penicillin and 100g/ml of streptomycin) streptomycin), at 37◦C in a humidified atmosphere of 5% CO2. Citotoxicity assay: The viability cell was performed by colorimetric assay MTT, according to the method of Mosmann. L929 cells were incubated (24h, 37°C and 5% CO2) into 96-well plates. This study used the HHEAs and alkaloidal fraction at the concentration of 0.4, 0.8, 1.6, 3.12 and 6.25mg/mL. Absovance was measured in an ELISA microtiter plate reader at 570nm. Viability was defined as ratio (%) of absorvance o treated cells to untreated cells. Acute Oral Toxicity: Was done according to OECD and adopted the fixed dose procedure. Results and discussion: Only C2

alkaloidal fraction showed a small but significant cytotoxicity (concentrations higher than 800 µg/mL). The extract not only cytotoxicity but also did not showed any obvious sign of toxicity in acute oral dose of 5000 mg/mL, indicatiing that these preliminary results , the extract did not show any apparent toxicity. Conclusion: The HEEAs was not cytotoxic, and also did

not cause any apparent toxicity. Financial support: FAPESPA and CAPES.




39

CODE 39.2 ACUTE ORAL TOXICITY EVALUATION OF THE DRY ETHANOLIC

EXTRACT OF Himatanthus articulatus AND Eleutherine plicata Vilhena, T. C.1; Silva, T. L. 1; Gomes, L. F. S.2; Vasconcelos, F.3; Percário, S4; Pereira, W. L. A5. 1 Mestrando no Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém-Pa. 2 Mestre em Ciências Farmacêuticas pelo Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém-Pa. 3 Laboratório de Toxicologia, Faculdade de Farmácia, Universidade Federal do Pará, Belém-Pa.4 Laboratório de Pesquisa do Estresse Oxidativo, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-Pa. 5 Laboratório de Patologia Veterinária, Universidade Federal Rural da Amazônia, Belém-Pa. Corresponding author: [email protected] Introduction: In Brazil, the use of natural products for medicinal purposes dates from before its colonization. The natives used these products for healing and in different religious rituals. Today, this knowledge have been adapted to the needs of the urban populations that acquire these products in markets, and is attracting the attention of researchers who studies the therapeuct potentials of natural products, as well as the identification and isolation of bioactive substances. In this context, products obtained from Sucuuba (Himatanthus articulatus) and Marupazinho (Eleutherine plicata) are used for the treatment of malaria and amoebiasis, respectively; but there are only a few studies of the toxic effects of these species, an important item in the evaluation of the security in the use of medicinal plants. Objective: Evaluate the acute oral toxicity of the dry ethanolic extract from the barks of H. articulatus (EEHa) and bulb of E. plicata (EEEp). Matherial and Methods: The barks of H. articulatus were collected in Marapanim/PA, in the month of june; the bulbs of E.plicata were collected in Traquateua/PA in September; both of them were identified by researcher of the Emílio Goeldi Museum, Dra. Marília Coelho Ferreira. After the collection, the materials were washed, dried and pulverized, and then a aliquot of the drug from both species was used in the pharmacognostic and physicochemical tests. Next, 1kg of each drug were used for the extraction of the metabolites. The method of choice was percolation in 5 L of alchohol 96° GL. The obtained extracts were filtered and concentreted in rotary evaporator. A part of these was used in phytochemical analisys, and the other part was lyophilized. With the EEHa and EEEp, was made the evaluation of the acute toxicity, in Swiss young mice from both genders, following the OECD 420. Results and Discussion: The EEH nd EEEp (5000 mg/kg) d dn’ show ny s gn of toxicity in mice, except for the diarrhea cases in the animals treated with EEHa, on the firsts 24 hours of tests, which was corroborated by the absence of hystopathological modifications. Conclusion: The EEHa and EEEp showed little toxicity on the major dose used on the test. FINANCIAL SUPPORT: master scholarship from CAPES/Brazil



40

CODE 40.1 HISTOPATHOLOGICAL STUDIES OF THE EFFECTS OF THE

AQUEOUS EXTRACT OF THE Orbignya phalerata Mart (BABASSU) MESOCARP POWDER IN ADULT MICE TREATED ORALLY Silva, A. P. S.1; Barros, I. C1; Coêlho, M. L.1, Rios, V. M2; Policarpo, P. R2; Araújo, S. S.3; Borges, E. M. M.3; Tomé, A. R.4; Nunes, L. C. C.1*; Freitas, R. M.1*

1Laboratory of Experimental Neurochemistry Research, Pharmaceutical Technology Center, Federal University of Piauí. (NTF/LAPNEX/UFPI). 2Pharmaceutical Technology Center, Laboratory of Experimental Neurochemistry Research, Federal University of Piauí (NTF/LAPNEX/UFPI). 3Laboratory of Experimental Neurochemistry Research, Federal University of Piauí (NTF / LAPNEX / UFPI) 4State University of Ceará (UECE). Corresponding author: [email protected] Introduction: The babassu mesocarp (Orbignya phalerata Mart) produces a powder that is commonly used among communities in the Northeast of Brazil as medicine and food supplement, yet without the existence of data on its efficacy and safety for human consumption. Objective: The effects of the acute oral administration of the powder aqueous extract (PAE) obtained from the mesocarp of babassu were investigated through the histopathological analysis in adult Swiss mice. Materials and methods: The animals (n=10/group) were divided in four groups and were treated orally with doses of 1, 2 and 3 g/kg with the PAE, respectively group 1, 2 and 3 and, to the fourth group, the control group, a 0.9% saline solution was administered. After 30 days of observation, all groups were euthanized and their brains were removed for histopathological analysis performance. The severity of the injury was expressed as mean ± SEM (standard error of the mean) of the scores of damage. Results and Discussion: The groups treated with babassu PAE were not able to promote injuries worthy of note, as the neurons of the hippocampus region presented morphology in the pattern of nerve cells, with spherical nuclei and central nucleoli, with no vacuolization or polymorphisms. The areas of the striatum were well characterized, without areas of necrosis or vacuolization. There were no significant differences between the test groups and the control group. Conclusions: Thus, under the conditions of the study, the babassu PAE did not produce toxic effects in the animals exposed to it.




41

CODE 40.2 THERMAL CHARACTERIZATION OF BABASSU (Orbignya phalerata

Mart) MESOCARP POWDER COMPARED WITH THE RAW MATERIAL MICROCRYSTALLINE CELLULOSE Barros, I. C.1; Silva, A. P. S1; Coêlho, M. L.1, Policarpo, P. R.2; Freitas, R. M.1*; Nunes, L. C. C.1

1Post-Graduation Program in Pharmaceutical Sciences, Pharmaceutical Technology Center, Laboratory of Experimental Neurochemistry Research, Federal University of Piauí. (NTF/LAPNEX/UFPI), Teresina - Piauí, Brazil. 2Scientific Initiation Program - Bachelor of Pharmacy, Pharmaceutical Technology Center, Laboratory of Experimental Neurochemistry Research, Federal University of Piauí (NTF / LAPNEX / UFPI), Teresina – Piauí, Brazil. Corresponding author: [email protected] Introduction: The thermal analysis is used in the pharmaceutical industry as a fast and reliable technique on the development of new pharmaceutical formulations. Objectives: The thermal characterization of the powder of babassu mesocarp (BM) (Orbignya phalerata Mart.) and the comparison with the microcrystalline cellulose. Materials and methods: The curves of differential thermal analysis (DTA) and the thermogravimetric analysis (TG) were obtained by a differential thermal analyzer in an atmosphere of N2, flow of 50mL.min-1 and a heating rate of 10°C.min-1 up to 600°C. Results and discussion: The BM DTA curve showed an endothermic process in the range of 30°C to 80°C (humidity loss) and two sequential endothermic processes in 283.40 to 348.12°C and from 424,09 to 456.52°C (decomposition of the material). On the BM TG, three events of mass loss at temperatures corresponding to the DTA were observed. In the DTA of cellulose, a loss of surface water in 39.08°C to 93.94°C (confirmed by TG) and another endothermic event around 370°C (decomposition of the excipient) were detected. On the TG, a large mass loss (65.57%) of 342.47°C up to 388.40°C and the decomposition of the rest of the material in the range of 388.40 ° to 571.15°C were noted. Thus, it was perceived the superposition of some events. Conclusions: The babassu mesocarp presented thermal characteristics similar to the microcrystalline cellulose.




42

CODE 41 STUDY AND CHARACTERIZATION SPECTROSCOPIC OF THE

COMPLEX POTASSIUM HEXACYANOFERRATE OLIVEIRA, S. D. S.; TEXEIRA, L. L.; PONTES, D. L. Introduction: Coordination or complex compounds are substances that have a central atom or ion, bound to atoms, ions or groups known as ligands. His study is necessary due to its diverse applications. A very important ligand in the coordination chemistry is the cyanide ion (CN-). Being a strong ligand, together with the carbonyl originates stable complexes with well-known low-spin configuration, for example, hexacyanoferrates. Objective: This work aims to study the spectroscopic characteristics (UV-Vis and IR) of the iron complex: K3[Fe(CN)6] as well as carry out the study of symmetry. Material and Methods: Applying the symmetry operations were able to identify the point group to which the compound belongs. Using this information, it has been obtained the theoretical number of active bands in the infrared region for the complex studied. The vibrational spectrum of the complex K3[Fe(CN)6] has shown only one band n(CN) at 2122 cm-1. The electronic spectrum of the complex K3[Fe(CN)6] 7 has shown absorption bands: 205, 262, 281, 303, 325, 404 and 429 nm. Conclusion: It has been concluded that the complex K3[Fe(CN)6] presented the same number of bands in the infrared, obtained in theoretical study of the symmetry group.



43

CODE 42 MEASUREMENT OF TOTAL FLAVONOIDS IN THE

HYDROALCOHOLIC EXTRACTS OF RED PROPOLIS FROM ALAGOAS Marta, M. F. S1; Firmino, T. T. D.1; Vieira, D. A.1; Azevedo, E. P.1; Nascimento, T. G.2; Diniz Basílio, I. Jr.1

1Laboratório de Tecnologia e Controle de Medicamentos, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas (LabTCon/ESENFAR/UFAL). 2Laboratório de Controle de Qualidade de Medicamentos, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas. Corresponding author: [email protected] Introduction: Propolis presents a mixture of gum, resins and balms originated form diferents vegetals sourcers. Propolis stands out for its antibacterial, antifungal, antiviral, cytostatic and immunostimulant activity which justify its utilization since remote times.Objective:The work consisted in the application of the Spectrophotometry of UV-VIS in order to quantify of total flavonoids in hidroalcoolic extracts of red propolis from Alagoas using spectrophotometry UV-VIS. Materials and Methods:The red propolis was obteined from apiaries of mangrove region of Marechal Deodoro city. The validation of the method of analysis of extracts by ultraviolet spectrophotometry was made after the determination of the total flavonoids. To validate the methods were respected parameters of linearity, interval, accuracy, specificity, accuracy, robustness, limit of quantification and detection limit. Results and Discussion: The sample showed low levels of waxes with water content between 2,99 and 4,83% and 0,67-1,33% for ash content. The scan data revealed the maximum absorption at 425 nm, corresponding to the region of absorption of total flavonoids. The validation results showed to be linear in the concentration range 0.004-0.012-1 mg.mL. The limit of detection and quantitation were 0.00188 and 0.00572 mg.mL-1 respectively. The average content of total flavonoids presented by red propolis from Alagoas was 9.68%. Conclusions: Results obtained in the pre-validation showed that the method meets the requirements of accuracy and linearity, demonstrating reliability required for an analytical method, therefore, considers the method validated. Financial support from CNPq/Brazil



44

CODE 43 COMPARISON OF METHODS FOR DETERMINATION OF CONTENT

PROTEIN IN THE AQUEOUS AND ACETONE EXTRACT Annona squamosa Pinto, Y. S.1; Campos, R. S.1; Araújo, N. K2; Pagnoncelli, M. G. B. 1; Assis, C. F.1

1Laboratório de Bromatologia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte.2 Laboratório de Engenharia Bioquímica, Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte Corresponding author: [email protected] Introduction: Spectrophotometric determination of total protein is used in several areas such as clinical analysis, food science and technology, biochemistry, protein chemistry, physiology. The Annona squamosa is native to tropical America and produces edible fruit that originate medicinal products. The fruits usually contain proteins essential for the body. Thus, quantification is essential for the insertion of the fruit in the diet. Objective: The aim of this work was to quantify the content protein (aqueous and acetone extracts) using spectrophotometric methods. Materials and Methods: Annona squamosa were obtained from supermarket, in Prudente de Moraes Avenue, Rio Grande do Norte, Northest in Brazil, and stored at 5°C. Then, aqueous and acetone extracts were made from the peel and seed. The three methods were used: BCA, Lowry and Biureto methods. Results and Discussion: The acetone showed greater efficiency in the extraction of proteins than water, due to ability of acetone precipitate proteins. Then the acetone extracts showed higher values of the total content protein. According to the methods used, BCA method was more sensitive than Lowry and Biureto. The content of protein in pell (30g protein/100g pell) was higher than seed (1,2 g protein/100 g seed). Conclusion: The higher amount of protein was found in pell and the more sensitive methods for determination protein were BCA, showing that pell in foods presented important nutrients in human diet. Financial support from REUNI/Brazil



45

CODE 44 PHYSICO-CHEMICAL PARAMETERS OF LANETTE® AND POLAWAX®

EMULSIONS FOR “MANOEL CASADO DE ALMEIDA”/UFCG, PB PHARMACY SCHOOL PRODUCTION Silva, A. M. S.1,3; Souza, J. B. P.2,3; Alencar, J. S.2,3

1Farmácia Escola Manoel Casado de Almeida, Faculdade de Farmácia, Universidade Federal de Campina Grande (UFCG). Corresponding author: [email protected] Introduction: The Pharmacy School, a UFCG supplementary organ, will serve as a training field for pharmacy students, providing medicines and cosmetics handled to Seridó and Curimataú Paraiban regions. Therefore, the physico-chemical parameters it is important to the development and achievement products to be handled. For emulsions production was used ionic and nonionic waxes such as Polawax® and Lanette®, respectively. It is important to know the product behavior to ensure its physico-chemical and sensory characteristics. Objective: To evaluate creams and lotions Lanette® and Polawax® waxes based physico-chemical characteristics for “Manuel Casado de A me d ” Pharmacy School / UFCG, PB production. Material and Methods: The formulation used was Lanette® 5% and 10% and Polawax® 10% and 15% for lotion and cream, respectively. For the physico-chemical analysis were performed centrifugation, spreadability, viscosity, electrical conductivity, microscopic analysis and pH tests, and macroscopic homogeneity aspects, odor and color. Results and Discussion: The lotions Lanette® and Polowax® pH were within the standard range (between 5.5 and 7.0), however, the creams showed a slightly lower pH. In Spin, only the lotion Lanette® showed two phases, it was the only formulation with little uniformity under microscopic vision. Lanette® emulsions showed greater spread and higher electrical conductivity. Conclusion: The research showed the importance of physico-chemical evaluation of emulsions, demonstrating that some parameters should be optimized as the pH of creams and the Lanette® lotion concentration should be adjusted to achieve greater stability, as well as improve macroscopic characteristics.



46

CODE 45 MICROBIOLOGICAL ANALYSIS OF WATER FROM DIFFERENT

SOURCES OF FORTALEZA IN THE PERIOD JANUARY TO SEPTEMBER 2011. Assis, C. N.¹,*, Thé, M. F. D. S.¹; Costa, M. A. R.¹; Carvalho, M. M. F.¹, Oliveira, Y. S.¹; Bezerra, R. G.¹; Souza, F. M. B.¹ ¹Department of Pharmacy, Federal University of Ceará, Fortaleza/CE-Brazil Corresponding author:[email protected] Introduction: The water is currently one of the major global concerns regarding its bacteriological quality. The decree Nº 518 of March of 2004 of Ministry of Health establishes the control and surveillance of water quality for human consumption and its potability standards. Therefore, it becomes extremely important to search for bacterial indicators of water quality in order to avoid any problems caused by ingestion or direct contact with contaminated water. Objective: Evaluate the presence of Total and Fecal Coliforms and P.aeruginosa in water intended for human consumption. Materials and Methods: We analyzed 136 water samples in the period January-September 2011 by chromogenic substrate method for the investigation of Total and Fecal coliforms and Seeding Technique in Cetrimide Agar for the investigation of P.aeruginosa. Results and Discussion: Among the samples analyzed, 26.47% were reproved due to the presence of Total Coliforms and 37,78% of these had Fecal Coliforms. Of the samples analyzed, the highest incidence occurred in samples from wells in the region (38.89%). This index indicates that the water that does not undergo previous treatment should not be used by local people. It was detected the presence of P.aeruginosa in one sample. According to the legislation, the presence of these microorganisms evidence that the water is unfit for human consumption. Conclusion: A considerable percentage of water consumed in the local evaluated presented contamination by Total and Fecal Coliforms. Therefore, the authorities should attempt to carry out measures to improve quality of the water commonly used in Fortaleza.



47

CODE 46 UTILIZATION OF Mg, Al LAYERED DOUBLE HYDROXIDES (LDHs) AS

A CARRIER OF DIGOXINE FOR VECTORIZATION IN CHEMOTERAPY OF CANCER Santos, L. D. A.1; Barbosa, V. T.1; Grillo, L. A. M.2; Magalhães, N. S. S.3; Dornelas, C. B.1

1Laboratório de Tecnologia e Controle de Medicamentos (LabTCoM), Curso de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas – UFAL. 2Laboratório de Biociências Farmacêuticas (LabBFar), Curso de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas – UFAL. 3Laboratório de Imunopatologia Keiko Asami (LIKA), Universidade Federal de Pernambuco – UFPE. Corresponding author: [email protected]

Introduction: The search for alternatives in anti-tumor therapy involves the achievement of target-specific drugs for greater efficacy and fewer side effects. Digoxin is a very established drug in cardiovascular therapy, but recent reports also indicate its success in clinical use in chemotherapy of prostate and breast cancer. It will be the drug-test in a LDH nanocarrier system, working with folate, present specifically in tumor cells cited. Objective: This work presents the results obtained from the first step, the synthesis of LDH. Material and Methods: The LDHs will be characterized by: XRD, porosity, TG, FTIR and TEM in a collaboration of UFPE. Results and Discussion: The first two tests are already concluded and the rest are ongoing. The values of interplanar distances (d) were calculated and the main Miller indices from the corresponding plane to each obtained value of d were identified. They were similar to those assigned to hydrotalcite in the mineralogical manuals. d of the plane (003) can fluctuate between 7,6-7,8Å, depending on the hydration degree, a value of 7,86Å was obtained. The values of 4,48m²/g for the specific superficial area, 14,80nm for the diameter and 0,012cm³/g for the volume of pores were determined by the N2 adsorption isotherm.The analysis of the diffractogram suggests the formation of Mg, AI LDH; the porosity test provides greater details of the obtained material. Only with the other methods of characterization it will be possible to assert the success of the synthesis. This step is fundamental for antimonial-LDH nanocomposites development.

Financial support: CNPq and FAPEAL/Brazil.



48

CODE 47 CREATION OF DIGITAL'S MAGAZINES ON RATIONAL USE OF

DRUGS: NEW PERSPECTIVES ON EDUCATION. Silva, L. C.1; Cardoso, E. T. C.1; Silva, A. D. V.1; Pinheiro, A. M.1; Pontes,

A. C. A. de A.1; Souza; S. C. C.1; Campos, D. M.1; Luz, D. A.1; Silva, J. V.

S.1; Silva, M. L.1; Silva, M. C. M.1; Bastos, M. L. C.1; Sarmento1, R. M.;

Carvalho, M. N.3; Dolabela, M. F.2

1Integrante do Grupo PET Farmácia da Universidade Federal do Pará (UFPA). 2Farmacêutica, Professora do Curso de Farmácia da UFPA, Tutora do PET-Farmácia. 3Farmacêutica colaboradora – CEAF. Grupo PET-Farmácia (UFPA). Corresponding author: [email protected] Introduction: Acco d ng o Wo d He h O g n z on, ‘A on use

exists when patients receive medications appropriate to their clinical

needs, in doses that meet their own individual requirements, for an

adequate period of time, and at the lowest cost to them and their

commun y’. Educ ve c on wh ch nvo ves he op c of on use h s

contributed for reduce of the irrational use of medicaments. Objective: The

group PET- FARMÁCIA-UFPA is building a website containing a series of

characters and environments, with the purpose of raise population

consciousness about dangers of incorrect use of medicines. Results and

Discussion: The site is in construction stages where members of the

group are elaborating the stories and creating the characters as the

pharmacist, the hipocondriaco, among other. The program for creation of

vectors Corel Draw X5 is being used to create the character and after will

be used the program Adobe Flash Player 11.0.1.15 to create animation. All

will be available to population though a site with periodic update. In this

way, it is intended, by means of interactivity, stimulate the rational use of

medicines.



49

CODE 48 CHRONIC, BUT NOT ACUTE, ETHANOL WITHDRAWL AFTER

CHRONIC CONSUMPTION PROMOTES DEPRESSIVE-LIKE BEHAVIOUR WITHOUT ALTERING LOCOMOTION IN RATS. Ali, M. S.1; Sousa Pinto, I. A.1; André, E.1; Padovan, C. M.2; Gavioli, E. C.1; Soares-Rachetti, V. P.1 1 Depto. de Biofísica e Farmacologia-CB/UFRN,2 Depto. de Psicobiologia FFCLRP/USP. Corresponding author:[email protected] Introduction: Chronic abuse of ethanol can generate depression-related symptoms [J Consult Clin Psychol. 78(3):362-74, 2010]. Such symptoms persist over time and contribute to relapse [Alcohol Health Res World 22(1):61-6, 1998]. Objective: Observe if the withdrawal of ethanol after chronic consumption generates depressive-like behavior on forced swimming test (FST) or alters locomotor activity in rats. Materials and Methods: Male Wistar rats were subjected to chronic consumption of increasing concentrations of ethanol (2%, 4% and 6%), for 21 days and the control group received water ad libitum. In day 21, ethanol was replaced by water (withdrawn) and 3, 4 and 5 days (experiment 1) and 20, 21 and 22 days (experiment 2) were submitted to open field, pre-test and FST, respectively. FST is based on animal exposure to a cylinder (30cm diameter) with water (25 cm in height) at 24 ± 1 º C for 15 and 5 min in the pre-test and test session respectively. Results and Discussion: Data showed h e h no w hd w d dn’ e he ocomo o c v y of animals in open-field test for both experiments. In relation to immobility time in the FST, the ethanol group (experiment 1) showed no difference from the control group. In experiment 2, the ethanol group showed increased immobility time when compared to control [mean ± standard error of average time (in seconds); Control: 117.43 ± 17.30; Ethanol: 190.56 ± 10.70, n = 7-9].Conclusion: Ethanol withdrawal after chronic intake leads depressive-like behavior after chronic withdrawal but not acute. Further studies are required and can be useful to propose new therapeutic strategies in addiction. Financial support: PROPESQ-UFRN.



50

CODE 49.1 APPLICATION OF THERMOANALYTICAL METHODS IN EVALUATING

THE BEHAVIOR OF TUBERCULOSTATICS DRUGS PRODUCED BY NUPLAM / UFRN: ISONIAZID AND PYRAZINAMIDE Gurgel, R. K. C.1; Valença, R. C. A.1; Moura, M. F. V.1

1Laboratório de Química Analítica e Meio Ambiente, Departamento de Química, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Some medicines are marketed in combination, such as the tuberculostatics. Therefore, it is necessary to use useful tools such as thermoanalytical methods to assess the compatibility among drugs that are in binary mixtures. Objective: To evaluate the thermal behavior and the possible interactions of the binary mixture isoniazid / pyrazinamide, which is in different proportions, through the interpretation of TG, DTG and DTA curves. Materials and Methods: DTG-60 system from Shimadzu was used. It consists of one differential thermal analyzer coupled to a thermal balance for obtainment of measurements, and the TA60 software from the equipment manufacturer, for the treatment of the data. Results and Discussion: Through analysis of the data obtained from the TG curves, it was observed that the thermal decomposition of pyrazinamide alone occurred in two stages, with mass loss. To isoniazid alone, the event occurred in a single stage, given that pyrazinamide had become more stable, with a difference of almost 50.0 ºC. The curves of differential thermal analysis, however, showed a melting point in a temperature lower h n son z d’s. Fo m x u es obse ved, he me ng po n s we e owe than those of the individual drugs, the TG curve showed decomposition behavior profiles at higher temperatures than those observed for pyrazinamide, and lower than those observed for isoniazid. Conclusions: B sed on he esu s, ’s ssumed h hese d ugs h s some fo m of interaction, which will be investigated in future studies involving mixtures of these and other analytical techniques such as infrared spectroscopy. Financial support from Propesq/Brazil and master scholarship from REUNI/Brazil.




51

CODE 49.2 DETERMINATION OF PHYSICAL AND CHEMICAL COMPOSITION FOR

THE QUALITY CONTROL OF MILK CONSUMED IN THE CITY OF NATAL / RN Gurgel, R. K. C1; Valença, R. C. A1; Silva, E. I. L1; Carvalho, J. R. P1; Pagnoncelli, M. G. B1; Assis, C. F1

1Laboratório de Bromatologia, Departamento de Farmácia UFRN, Universidade Federal do Rio Grande do Norte (UFRN) Corresponding author: [email protected] Introduction: Milk is an essential food for human health because it is made up of proteins, carbohydrates, fats, minerals and water. Thus, its quality control is fundamental for ensuring the health of the population. Objective: To evaluate the quality of four samples of milk, raw and pasteurized, by reason of minimum standards required by the Ministry of Agriculture. Materials and Methods: The samples were chosen randomly, one of them being from the city of Barcelona / RN. The following tests were performed: density, acidity, determination of lipid content by Gerber method; dry residue by the indirect method and nonfat dry residue by the direct method; Alizarol test, test of reduction of methylene blue (TRAM) and the peroxidase test. Results and Discussion: Two samples were approved for human consumption. 50% were tampered with sugar, 100% had the density according to the reference values; 100% were above the permissible acidity; for the tests of dry and nonfat dry residue, 25% and 50%, respectively, had values above the minimum allowed; 25% did not contain the peroxidase enzyme, since the sample was of the long life type; for the tests TRAM and Alizarol, 50%, both tests showed normal values, according to the reference; 100% of the samples showed lipid content within the allowed for each type. Conclusions: In most of the milk samples various irregularities in the physical-chemical aspects were identified, therefore, it becomes evident the need for measures to improve the quality of the milk consumed in the city of Natal / RN. Financial support from UFRN/Brazil.




52

CODE 50 STUDY OF PHYSICOCHEMICAL PROPERTIES AND ACUTE TOXICITY

EVALUATION OF ETHANOLIC EXTRACT OF THE LEAVES FROM Mikania glomerata SPRENGEL. Santana, L. C. L. R.1; Brito, M. R. M.1; Sousa, G. F.1; Freitas, R. M.1,*

1Laboratório de Pesquisa em Neuroquímica Experimental do Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal do Piauí. Corresponding author: [email protected] Introduction: The Mikania glomerata is a plant belonging to the Asteraceae family that is widely used in folk medicine due to the actions bronchodilator, asthma, expectorant and antitussive. Objectives: The purpose of this study was to determine the physicochemical properties of the powder obtained from the leaves of M. glomerata, and to evaluate the toxicity in mice after acute treatment with repeated doses of a standardized ethanol extract prepared from the leaves of this specie. Material and Methods: During the study of physicochemical properties of the powder obtained from the leaves of M. glomerata was made to determine the bulk density and compaction, the total ash content, moisture content and particle size, and gross morphological analysis was performed and evaluated the acute toxicity with repeated doses on biochemical and hematological parameters of mice. Results and Discussions: In the physicochemical analysis, the powder flow properties presented. In studies of acute toxicity, the therapeutic dose suggested none alterations in parameters evaluated and found 50% lethal dose (LD50) of the extract was much higher (~ 3000 mg kg-1). Conclusions: According to the results obtained we suggest that the powder can be used in formulating a solid dosage form that the ethanolic extract can be used safely in humans, since it presented a value for the LD50 that can be classified as harmful, and produced no morphological changes in major organs, biochemical and hematological parameters of mice. Financial support: CNPq e FAPEPI



53

CODE 51 EPIDEMIOLOGY OF CANDIDEMIA IN PUBLIC HOSPITALS OF

ALAGOAS, BRASIL Mendonça, N. M.1; Lima, F. S.2; Maranhão, F. C. A.1 1Laboratório de Microbiologia Geral e Clínica, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas (LMGC/ICBS/UFAL). 2Centro de Patologia e Medicina Laboratorial (CPML). Corresponding author: [email protected] Introdution: Species of the Candida are opportunistic pathogens most frequently involved in superficial and mucous-cutaneous mycoses, however is an important cause of bloodstream infections. Candidemias rates vary geographically like a systemic disease associated with high mortality in nosocomial ambient. Objective: Evaluation of Candida spp. incidence in blood samples obtained in the major public hospitals of Maceió, Alagoas (Brasil). Materials and Methods: During the period studied (April to December 2010) we analyzed the frequency of Candida spp in patients treated in three public hospitals of Maceió. Candida species were identified through standard microbiological methods in suspect blood samples by use of Sabouraud Agar (24- 48 h a 37°C), Gram stain in microscopy followed by cultive in CHROMagar Candida® (24- 48 h a 35°C) for presumptive identification. Results and Discussion: In a total of 2346 blood samples, 269 (11,47%) showed presence of microorganisms, being 17 identified like Candida species thought characteristics of colonies in media and localization of blastoconidia (40x). C. tropicalis was confirmed in 35.29% (6) and C. albicans in 23.53% (4), while had 11.76% (2) of C krusei and 29.41% of Candida sp (5), totalizing 29.41% of species non-albicans. Conclusions: The candidemia frequency was low in the period studied, with a prevalence of C. tropicalis followed by C. albicans. Candida non-albicans in fungemia are reemergents during nosocomial infections and the adequate identification is important to guide therapeutic choices, since there is resistance specie-related and the emergence of azole-resistant C. glabrata and C. krusei is an actual problem in hospitals.



54

CODE 52.1 VALIDATION OF ANALYTHICAL METHODOLOGY FOR Passiflora

incarnata L. EXTRACT BY HPLC AND ANALYISIS OF COMMERCIALS EXTRACTS FROM RS. Wildner, A. C.1; Meneghini, L. Z.1; Hexsel, H. T.1; Gnoatto, S. C. B.1; Bergold, A. M.1

1Laboratório de Química Farmacêutica, Faculdade de Farmácia, UFRGS Introduction: The extracts of Passiflora incarnata L. have become very important for the therapeutic of mind diseases. This species is considered as an official plant by WHO, ANVISA and many official codes such European Pharmacopoeia (2002) and French Pharmacopoeia (2007). However, the Farm. Bras. V, 2010, does not support information about this species. Therefore, the present study intents to develop an analytical method to assay of P. incarnata L. extracts appropriate for quality control. Objective: The objective of this study is optimize the HPLC method for analysis of vitexin, a C-glycosil flavonoid, in Passiflora incarnata L. extract; also evaluate the content of commercial extracts available in Rio Grande do Sul. Materials and Methods: The HPLC analyses were performed using a Shimadzu chromatograph, an isocratic system, C-18 column and security guard cartridges. Results and Discussion: For the qualitative analysis the retention times and UV spectra of samples were compared with those of reference standards. The method had validated and applied to quantitative analysis of five commercial formulations of RS. Conclusions: The experimental results proved that the proposed HPLC method is selective, precise, accurate, robust and therefore suitable for detection, separation and quantification of flavonoid constituents and derivatives related to vitexin, present in extractive samples. This method was also employed to analyze commercial samples. Of the seven P. incarnata L. ex c s n yzed 40% d dn’ h d v ex n, v d nd c o of authenticity, and 60% had levels between 0.008% a 0. 031% of vitexin. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil.



55

CODE 52.2 DEVELOPMENT AND VALIDATION OF A METHOD OF DISSOLUTION

FOR DARIFENACIN EXTENDED-RELEASE TABLETS BASED ON IN VIVO DATA Meneghini, L. Z.1; Wildner, A.C.1; Junqueira, C. A.1; Adams, A. I. H.2; Fröehlich, P. E.1; Bergold, A. M.1

1Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul-UFRGS. Porto Alegre, RS, Brasil. 2Universidade Federal de Santa Maria - UFSM, Centro de Ciências da Saúde, Depto. Farmácia Industrial, Santa Maria, RS, Brasil. Introduction: Several antimuscarinic drugs are on the market like darifenacin, chemically (S)-2-{1-[2-(2,3-dihydrobenzofuran-5-yl)ethyl]-3-pyrrolidinyl}-2,2- diphenylacetamide hydrobromide. It is a potent muscarinic receptor antagonist, available as a hydrobromide salt in Enablex®, an extended-release tablet which contains 7.5 mg or 15 mg of the drug. However, there is not pharmacopoeial method for dissolution test. Objective: The aim of this work was to develop and to validate a dissolution test for the Enablex® extended-release tablet based on in vivo data according to current FDA and USP guidelines. Materials and Methods: The sink condition was evaluated and a factorial design 24-1 was employed for method development. In vivo data were obtained from the literature and fraction (and percentage) of dose absorbed (FA) was calculated using Wagner- Nelson method. Results and Discussion: The best dissolution conditions were achieved using a USP apparatus I (basket), with 900 mL of dissolution medium containing 2% SDS at 50 rpm. A successful linear regression model model of fraction of drug absorbed versus dissolved was achieved (R2=0.9997, p<0.05). The validation was performed with an LC/UV method and results for specificity, linearity, precision and accuracy were in accordance with guidelines.Conclusions: The proposed method allowed achieving a level A correlation with the fraction of dose absorbed. The results showed that the test is adequate to evaluate the in vitro profile for extended-release darifenacin tablets. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil.



56

CODE 53.1 Lobophora variegata AND ITS ACTIVITY IN PAW EDEMA AND

HEPATOTOXICITY IN RATS Will, L. S. E. P.1; Castro, A. J. G.1; Nascimento, M. S.1; Magalhães, J. E. M.1; Pinheiro, T. S.1; Almeida, H. W. B.1; Costa, T. G.1; Medeiros, H. N.1; Leite, E. L.1 1Laboratório de Glicobiologia Vegetal, Departamento de Bioquímica, UFRN, Rio Grande do Norte, Brazil. Corresponding author: [email protected] Introduction: Seaweeds or marine algae have been reported to contain many important compounds which act as antibiotics, laxatives, anticoagulants, anti-ulcer products and suspending agents in radiological preparations. The algae have also several pharmacological activities such as anticoagulant, anti-inflammatory, anti-edematous, antitumor and antioxidant activities. Objective: The aim of this study was to investigate the effect of sulfated polysaccharides (F0.8) from Lobophora variegata seaweed on some models of inflammation. Material and Methods: The paw edema and hepatotoxicity were assessment in Wistar rats. Paw edema was used to demonstrate the effect of fucan in the carrageenan-induced inflammation. For hepatotoxicity assay was used as induced of hepatic toxicity the carbon tetrachloride (CCI4). Results and Discussion: An protective anti-edematous effect of F0.8 decreasing of paw thickness at 25 mg/kg occur inhibition dose- dependent in 89.1±10.0%, 50 mg/kg inhibited in 91.1±7.1 % and 75 mg/kg inhibited in 100.0±1.4% the edema in the fourth hour of the experiment, demonstrating to be dose-dependent. The hepatoprotective effect observed showed an effective decrease (p < 0.001) in serum levels of indirect bilirubin (8±0.002%, 16±0.0008% and 64±0.0005%). Hepatics enzymes (AST, ALT, and y-Glutamyl Transferase) in plasm were measured and the best hepatoprotective efects was showed in the concentration 75 mg by animal body-weight (p < 0.05). Histological analysis with H&E confirmed the hepatoprotective effect of this polymer. Conclusion: The results lead us to conclude that the sulfated fucans of F0.8v have significant anti-inflammatory and hepatoprotective activities. Supported by: CNPq and CAPES.

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57

CODE 53.2 Β-GLUCANS OF CARIPIA MONTAGNEI MUSHROOM SHOWS ANTI-

INFLAMMATORY ACTIVITY: NEWS PERSPECTIVE OF PPAR ATION Will, L. S. E. P.1; Castro, A. J. G.1; Nascimento, M. S.1; Pinheiro, T. S.1; Magalhães, J. E. M.1; Almeida, H. W. B.1; Medeiros, H. N.1; Alves, M. G. C. F.1; Filgueira, L. G. A.1; Leite, E. L.1

1Laboratório de Glicobiologia Vegetal, Departamento de Bioquímica, UFRN, Rio Grande do Norte, Brazil. Corresponding author: [email protected]

Introduction: The receptors activated by peroxisome proliferators (PPAR) are the class of linked receptors with the regulation of inflammation. Was known that glucans act as anti-inflammatory influencing directly the phagocytic activity of immunomodulatory cells. Therefore, was verified the action of B Glucans extracted from the Caripia montagnei mushroom in inflammation PPAR way. Material and Methods: Cell viability of fibroblasts was analyzed by MTT, incubated with B-glucans and perfluorooctanoic acid (PFOA). The anti-inflammatory activity of polysaccharides, ligands of PPAR (GW9662, and ciprofibrate PFOA Rosiglitazone) Parecoxib, indomethacin and L-NAME was assessed by the pleurisy model and paw edema induced by carrageenan, being measured leucotaxis rate, nitric oxide levels and inhibition of paw edema. Also evaluated the synergy between the polysaccharides tested with the PPAR ligands, AINEs and L-NAME. Results and Discussion: Was observed a cell viability rate of 91.6 ± 4.5% when incubated for 24 h with B-glucan (50 μg/m ) and of 87.9 ± 0.3% when incubated with B-glucan + PFOA. The glucans (90 mg/kg) inhibited in 81.03 ± 07.03% the leucotaxis and in 47.31% the release of nitric oxide, compared with the control, the edema in 90.7 ± .5% in the fourth hour while indomethacin, Parecoxib, L-NAME and PFOA decreased by 92.4 ± .6%, 47.4% ± 1.7%, 78.5 ± 4.3% and 90.8 ±10.9%, respectively. L-NAME, Parecoxib and PFOA had synergy with B-Glucans tested. Other PPAR ligands tested inhibited in, or less than, 50%. Conclusion: Thus was verified an effective anti-inflammatory activity of B-glucans of Caripia montagnei, being this possibly PPAR way. Supported by: CNPq and CAPES.

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58

CODE 54 EVALUATION OF HYPOTENSIVE RESPONSES OF LYOPHILIZED

AYAHUASCA TEA IN SHR RATS Moura, M. T. D.1; Herculano, E. A.1; Costa, C. D. F.1; Oliveira, M. P.1; Netto, S. M.2; Ribeiro, Ê. A. N.1

1Laboratório de Farmacologia Cardiovascular, Escola de Enfermagem e Farmácia,UFAL, Maceió, AL, Brasil. 2Departamento de Psicobiologia, UNIFESP, São Paulo, Brasil. Corresponding author: [email protected] INTRODUCTION: Ayahuasca tea is a psychoactive drink of South American origin, used in indigenous rituals and the Amazonian region of Brazil certain religions. It is prepared by decoction of the vine Banisteriopsis sp ("mariri") with the leaves of the shrub Psychotria sp ("chacrona"), and species Banisteriopsis caapi and Psychotria viridis the most frequently used. In previous studies our group found hypotensive and vasorelaxant activity of tea ayahuasca in SHR rats (Herculano et al, 2011). OBJECTIVE: The aim of this study was is to elucidate the possible mechanism of action involved in hypotensive effects of lyophilized ayahuasca tea (LYT) in SHR rats, by in vivo approach. MATERIALS AND METHODS: For the measurement of blood pressure and heart rate, male spontaneously hypertensive rats (250-350 g) were anesthetized with thiopental (45 mg / kg, ip) and polyethylene catheters were inserted into the abdominal aorta and inferior vena cava for recording of pressure and drug administration, respectively. The same procedure was used to verify the participation of nitric oxide (NO) in the hypotensive effect by blocking the enzyme NO synthase. For this we used a blocker of NO synthase pathway, NG-nitro-L-arginine methyl ester (L-NAME, 20 mg / kg, iv). The experiments were performed 24 hours after surgery. The results are presented as mean ± standard error of mean. The statistical analysis used the "t" Student test with a significance of p < 0,05. RESULTS AND DISCUSSION: Intravenous administration of LYT (0.5, 1, 5, 10, 20, 30, 50, and 60 mg / kg, randomly) induced hypotension in doses of 20, 30, 50 and 60 mg / kg (-3, ± 1.5, -7.1 ± 0.8, -5.8 ± 2.0, -2.4 ± 1.3, -8.2 ± 0.6, - 18.4 ± 0.4; -9.5 ± 1.2, -22.8 ± 1.1 mmHg, respectively) and a slight bradycardia at doses of 0.5, 1, 5 mg / kg more pronounced at a dose of 60 mg / kg and tachycardia in doses of 10, 20, 30 and 50 mg / kg (-5.8 ± 0.5, -3.8 ± 1.1, -3.0 ± 0.8, 6.3 ± 1.7, 4, 4 ± 1.3, 11.8 ± 1.4, 13.0 ± 1.0 and -35.9 ± 0.3 bpm, respectively. After administration of NG-nitro-L-arginine methyl ester (L-NAME, 20 mg / kg, iv), the hypotensive response to LYT was significantly increased and heart rate was significantly changed, after nitric oxide (NO) synthase blockade (L-NAME, 20 mg/Kg, i.v.). CONCLUSIONS: These results demonstrate that tea promotes hypotensive effect in SHR rats. The L-NAME was also capable of significantly changing LYT-induced effects in rats SHR, suggesting that NO appears be participating of this effect. Sources of research support: FAPEAL, CAPES and CNPq.




59

CODE 55.1 ANGIOGENESIS INHIBITION AND B-GLUCANS ANTITUMOR ACTION

OF CARIPIA MONTAGNEI MUSHROOM Nascimento, M.S. 1, Castro, A.J.G. 1, Will, L.S.E.P.1, Almeida, H.W.B.1, Florentein, K.Q. 1, Paiva, A.A.O. 1, Costa, T.G. 1, Leite, E.L.1

1Laboratório de Glicobiologia Vegetal, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN), Rio Grande do Norte, Brazil. Corresponding author: [email protected] Introduction: B-glucans are homopolymers of D-glucose which have

bioactivity, such anti-inflammatory, antioxidant and antitumor properties, being the latter promoted by one possible antiangiogenesis action, which develop a critical function in the metastasis and growth of solid tumors. Objective: Therefore aimed to analyze the antiangiogenic and antirumor properties of B-glucans from Caripia montagnei mushroom. Material and Methods: Its antitumor action was analyzed by the MTT method, where

the HeLa cell line were incubated at the time 24 and 48 hours in presence or absence of glucans at different concentrations (25, 50 e 100 μg/ml). Was also used the chorioallantoic membrane method (MCA) to evaluate the antiangiogenic activity. Chicken eggs were incubate in a greenhouse, after access to MCA the glucans were administered about this, being counted the number of vessels formed after 24 hours and semi-quantified by score system. Heparin and spironolactone were used as positive and negative controls, respectively. Results and Discussion: Was observed

that the cytotoxicity rate for HeLa cells incubated in 24 hours period with C. montagnei polysaccharides was 83.4 ± 2.3 % e de 94.36 ± 2.3 % for 48 hours period (100 μg). The antiangiogenic activity showed that the 1000 μg/egg concen on of g uc ns p esen ed s gn f c n ng ogenes s inhibition rate, compared the heparin and spironolactone action. Conclusion: Thus, was noted that the C. montagnei B-glucans showed

significant antiangiogenic and antitumor activities, which proposed this mushroom as a potencial drug. Supported by: CNPq and CAPES.



60

CODE 55.2 CARIPIA MONTAGNEI MUSHROOM B-GLUCANS SHOWING NON

HEMOLYTIC ACTION AND ANTIOXIDANT ACTIVITY: POTENTIAL FUNCTION IN LIPIDIC PEROXIDATION INHIBIT AND OXIDATIVE STRESS Nascimento, M.S.1, Castro, A.J.G. 1, Will, L.S.E.P.1, Magalhães, J.E.M. 1, Dore, C.M.P.G.1, Almeida, H.W.B.1, Baseia, I.G. 2, Alves, L.G.F. 1, Leite, E.L.1

1Laboratório de Glicobiologia Vegetal, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN), Rio Grande do Norte, Brazil. 2Laboratório de Micologia, Departamento de Botânica, Ecologia e Zoologia, Universidade Federal do Rio Grande do Norte (UFRN), Rio Grande do Norte, Brazil. Corresponding author: [email protected] Introduction: The oxidative stress was caused by imbalance between the reactive oxygen species producion and antioxidant compounds, being favorable to free radicals liberation which can let to cellular aging, cardiovascular diseases and cancer, trigerred by among others, for lipid peroxidation which is consequent of oxidative actions in plasmatic membrane. Thus, compounds that can act like antioxidants had notorious physiological and medical importance. Objective: Therefore, aimed to analyze the antioxidant and non hemolytic properties of B-glucans extracted from Caripia montangnei mushroom. Material and Methods: The inhibition of Hydroxyl radicals action, Total antioxidant activity, Reducing power, Lipidic peroxidation, Activity of ferric ion chelation and Hemolytic activity of B-glucans in different concentrations were analyzed by spectrophotometry. Results and Discussion: Was verified significant antioxidant activity of the polysaccharides tested. 1 mg/ml concentration of B-glucans extracted from Caripia montangnei inhibits in 37.9 ± 0.01% the hydroxyl radicals formation, showing total antioxidant activity of 47.1% and reducing power of 50% with 4 mg/ml concentration, which compared withal ascorbic acid standard activity. With the same concentration observed reduction in 37.6±0.16% in the lipidic peroxidation process and ferric ion chelation rate of 95± 0.002%. Was also verified that all tested concen ons (25, 50 nd 100 μg/mL) showed no significant hemolytic, with the highest concentration showing 8.4±0.008% hemolysis, compared to positive control (Triton-X). Conclusion: Thus, was verified a significant antioxidant and non-hemolytic activity of glucans of the C. montagnei mushroom. Supported by: CNPq and CAPES.



61

CODE 56 DETERMINATION OF MOISTURE AND LIPIDS IN FRUITS OF Licania

tomentosa Beth (Oiti) Teixeira, L. L.¹; Amaral, N. K. M.¹; Macedo, J. B. M.²; Santos, E. C. G.²; Oliveira, S. D. S.¹; Moura, M. F. V.¹. ¹ Programação de Pós – graduação em Química – PPGQ ² Programa de Pós – graduação em Ciências Farmacêuticas – PPGCF Materials and Methods: Samples of the fruits of Licania tomentosa Beth (Oiti) were collected in February 2011 in the city of Natal - RN. Fruits were collected in relatively mature stage of ripening, a total of 226 fruits. The fruits were divided into peel, pulp and seed. Determination of moisture content is executed by the gravimetric method and for determination of lipids is used the Soxhlet extraction method. Results and Discussion: As a result we found that the average content of moisture to the peel was 60.5 g (+ / -1.6) in 100 g of sample, 72.3 g (+ / -0.9) in 100 g of pulp and 45.7 g (+ / -2.0) moisture in 100 g of seed. For the results of lipid was obtained 3.7 g (+ / - 0.09) of lipids in 100 g of peel, 0.4 g (+ / - 0.03) 100 g of lipids in the pulp and 0, 05 g (+ / -0.004) of lipids in 100 g of seed. Conclusions: Through these results we conclude that the peel is part of the plant with the highest level of lipids.



62

CODE 57 EVALUATION OF THE CARDIOVASCULAR EFFECTS OF Syzigium

cumini SEEDS Herculano, E. A.¹; Feitoza, P. R.¹; Nascimento, W. U. C.¹; Costa, C. D. F.²;

Aquino, P. G. V.²; França, P. H. B.; Araújo-Júnior, J. X.²; Sant'Ana, A. E.

G.²; Ribeiro, Ê. A. N.

¹ Escola de Enfermagem e Farmácia, UFAL, Maceió/AL, ² Instituto de Química e Biotecnologia, UFAL, Maceió/AL. Introduction: Syzigium cumini (Myrtaceae) is a plant popularly known as “b nco-de-v úv ” or “j me ão”. The spec e h s been desc bed n he literature as antifungal (J Antimicrob Chemother. 60; 312, 2007), anti-allergic (Braz J Med Biol Res 40; 1, 2007), anti-diabetic (Planta Med. 9; 139, 1986), and anti-mutagenic (Phytochemistry. 36: 1027, 1994). Objective: To investigate the acute cardiovascular effects of the ethanolic extract of the Syzigium cumini seeds (EESCS) in rats. Materials and Methods: The extract was prepared by the percolation method with 95% ethanol, followed by steam evaporation. Exactly 250 g of dry powder was percolated to get a net yield of 30.6 g of concentrated extract (12.24%). Male Wistar and SHR rats (250-350g total weight) were anesthetized with sodium thiopental (45mg/kg, i.p.). Polyethylene catheters were inserted into the lower abdominal aorta and into the inferior vena cava, for blood pressure measurements and administration of drugs, respectively. After a 24h period, experiments were performed in conscious unrestrained rats. The results are presented as mean±standard error of the mean. The study was approved by the ethics committee of the Federal University of Alagoas (010151/2008-82). Results and Discussion: The EESCS (0.5; 1; 5; 10; 20 and 30 mg.kg-1 i.v., randomly, n=5) in Wistar rats elicited immediate and dose-independent decreases in mean arterial pressure (MAP) (-6.2 ± 3.0%; -4.5 ± 0.1%; -10.4 ± 2.1%; -9.3 ± 0.6%; -7.6 ± 1.2%; -11.8 ± 4.2%, respectively) with low reduction in heart rate (HR) (-3.1 ± 4.2% ; -1.9 ± 1.9%; -1.5 ± 0.4%; -3.1 ± 0.9%; -5.5 ± 3.3%; -1.1 ± 1.1%, respectively). In SHR the effects hypotensive (-3.2 ± 1.0%; -3.9 ± 1.0%; -5.1 ± 0.9%; -3.8 ± 1.0%; -5.4 ± 1.1%; -6.9 ± 2.2%, respectively) and bradicardic were also observed (1.6 ± 0.9%; 0.6 ± 0.7%; 2.7 ± 1.2%; -2.0 ± 0.7%; -4.2 ± 1.1%; -4.4 ± 2.0%, respectively), however, the bradicardic effect just in higher doses 10; 20 and 30 mg/kg, only). Conclusions: These results suggest that the extract is able hypotensive actions with reduction in heart rate at all doses in normotensive rats and just higher doses in hypertensive rats probably due to a genetic differences between animals. Sources of research support: FAPEAL, CAPES and CNPq.



63

CODE 58.1 POLYSACCHARIDES EFFECTS FROM LOBOPHORA VARIEGATA IN

A TOPICAL INFLAMMATORY MODEL

Pinheiro, T.S.¹; Nascimento, M.S.¹; Magalhães, J.E.M.¹; Alves, M.G.C.F.¹; Castro, A.J.G.¹; Paiva, A.A.O.¹; Almeida, H.W.B.¹; Costa, T.G.¹; Florentin, K.Q.¹; Medeiros, H.N.¹; Leite, E.L.¹ ¹Laboratório de Glicobiologia Vegetal. Departamento de Bioquímica, UFRN, Rio Grande do Norte, Brazil. Corresponding author: [email protected] Introduction: Algae are capable to produce large variety of secondary metabolites and are considered rich sources of structurally diverse bioactive compounds with diverse biological activities as an anti-allergic, antitumor and anti-inflammatory. Objective: The aim of the present investigation was to investigate the action of sulfated polysaccharides in contact hypersensitivity reaction induced by croton oil. Materials and Methods: The algae passed through the collection process, spray, delipidation and differential, proteolysis and precipitation with increasing volumes of acetone (0.3-2.0). All fractions showed sulfated polysaccharides. Results and Discussion: The F0.8 fraction, showed a high yield in total weight (19.49%), low percentage of protein contamination (0.90%) and high levels of carbohydrates (66.2%) and sulfate (3.2%). Mouse exhibited very mild dermatitis when treated with croton oil or oxazolone. The Contact Reaction is an induction of irritating oil in local edema with topical treatment in ear mouse. Most animal models used are based on skin inflammation induced inflammatory responses in acute skin by application of agents such as croton oil. The animals ears were measured and histological analysis were made. It was found in treated groups with different concentrations the reduce swelling occurred in a dose-dependent. Dermal response was evaluated by H&E and immunohistochemistry. This initial study suggests the 0.8 fraction of algae L.variegata has anti-inflammatory and anti-allergic activities. Conclusions: These results suggest and are support, for the hypothesis that contact dermatitis is a classic delayed type hypersensitivity, and demonstrate the importance of the interaction between the irritant and algal that the irritant effect of chemicals may be mediated through the cutaneous innate immune system. Supported by CNPq and CAPES.



64

CODE 58.2 ANTIOXIDANT ASSAYS OF BRUTE EXTRACTED FROM

PLECTTRANTUS BARBATUS ANDR. Pinheiro, T.S.¹; Magalhães, J.E.M.¹; Will, L.S.E.P.¹; Castro, A.J.G.¹; Paiva, A.A.O.¹; Dore, C.M.P.G.¹; Costa, T.G.¹; Medeiros, H.N.¹; Filgueira, L.G.A.¹; Leite, E.L.¹ ¹Laboratório de Glicobiologia Vegetal. Departamento de Bioquímica, UFRN, Rio Grande do Norte, Brazil. Corresponding author: [email protected] Introdcution: Latest studies showing that oxidative stress was caused by disbalance between the reactive species of oxygen production and antioxidant compounds, and this is favorable to free radicals liberation. Thus, compounds that can acting like antioxidants had notorious physiological and medical importance. Objective: This study aimed analyze the antioxidant properties of the brute extracted from Plectranthus barbatus Andr. (Boldo Brasileiro). Materials and Methods: The brute extracted used was obtained by gradual fractionation with acetone, and the polysaccharides contained in this extracted were used in the antioxidant assays. The sequestration of DPPH radical, Superoxide radicals, Hydroxyl radical and inhibition of lipidic peroxidation in different concentrations were analyzed by spectrophotometry at 517 nm, 560 nm, 510 nm and 532 nm respectively. Results and Discussion: It was verified one significant antioxidant activity of the polysaccharides tested, which with 2 mg/ml concentration inhibits in 89.96% of the reaction involving DPPH radical. With the same concentration can inhibit 22.0% of superoxide radicals, and the inhibition of hydroxyl radicals formation was 75% in the same concentration. Lastly this concentration can inhibits 15% the lipidic peroxidation and all tests were compared with ascorbic acid standard activity. Conclusions: Thus, was verified a significant antioxidant activity of polysaccharides from Plectranthus barbatus. Supported by CNPq and CAPES.



65

CODE 59 EVALUATION OF DENTIN ROUGHNESS AFTER USE OF DIFFERENTS

CHELATING AGENTS EMPLOYED IN ENDODONTICS Tartari, T.1; Almeida, B.V.N.1; Silva Júnior, J.O.C.2; Duarte Júnior, A.P.2; Negrão, C3; Costa, C.E.F3; Pessoa, O.F.1; Silva e Souza, P.A.R1. 1Laboratório de Materiais Dentários, Faculdade de Odontologia, Universidade Federal do Pará (UFPA). 2Laboratório de P&D Farmacêutico e Cosmético, Faculdade de Farmácia, Universidade Federal do Pará (UFPA). 3Laboratório de Catálise e Oleoquímica, Faculdade de Química, Universidade Federal do Pará (UFPA). Corresponding author: [email protected] Introduction: The chelating agents used during endodontic treatment to promote the cleaning of dentinal walls may cause changes in surface roughness increasing the micromechanical bonding of root canal sealers. Objective: Evaluate the effect of different irrigation schemes employing chelating agents on dentin roughness. Materials and Methods: Fifty root fragments of human teeth were randomly divided, according to irrigation schemes, into five groups: G1- saline (physical control) for 30 minutes; G2- 2,5% sodium hypochlorite (NaOCl) for 30 minutes; G3- 2,5% NaOCl for 30 minutes followed by 17% ethylenediaminetetraacetic acid (EDTA) for 3 minutes; G4- 2.5% NaOCl for 30 minutes followed by 10% citric acid for 3 minutes; and G5-NaOCl 5% associated with 18% etidronate solution (HEBP) for 30 minutes. To compare the results, measurements of dentin roughness were made with a roughness tester before and after treatment with irrigating solutions. Results and discussion: Analysis of the results by T-Test showed that in G1 (p=0,29) and G2 (p=34) there were no changes in surface roughness. The groups G3 (p <0.0001), G4 (p <0.0001) and G5 (p <0.0001) had significant increase in roughness, being numerically superior in G5. The group using saline showed that irrigators do not exercise physical effect on the roughness, but this was significantly increased as a result of chemical action of chelating agents. The NaOCl did not alter the roughness, except when associated with HEBP, suggesting that NaOCl may potentiate the effect of soft chelators. Conclusion: Irrigation schemes which employing chelating agents cause significantly increase in dentin surface roughness.



66

CODE 61 TOPICAL APPLICATION OF PROPOLIS FOR HEALING OF WOUNDS

SURGICALLY INDUCED AND EVALUATION OF BIOCHEMICAL PARAMETERS IN WISTAR RATS. Costa, M. M1; Nascimento, J. S.1; Da Costa, Y. G. F1; Menezes, J. B.1; Bastos, M. L. A.3; Batista, L. L. V.2; Dornelas, C. B2; Grillo, L. A. M.1

1 Laboratório de Bioquímica e Biologia Molecular; 2Laboratório de Tecnologia e Controle de Medicamentos (LabTCoM); 3Laboratório de Tratamento de Feridas; 1,2,3 Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas, Brasil. Corresponding author: [email protected] Introduction: Propolis is considered a prominent natural product, known by its diverse biological properties. The action of propolis in tissue regeneration and granulation is currently suggested as a product that favors wound healing in addition to its natural antibiotic property, free from side effects and its low cost in comparison with other products currently applied on wounds. Nevertheless, little is known about of its use as healer of wounds, systemic absorption and alterations in the biochemical parameters. Objective: The scope of this study was to evaluate alterations on the biochemical parameters caused by treatment with the propolis for the wound healing. Materials and Methods: The methodology consisted of treatment of wounds in Wistar rats randomly divided into three groups of five animals: control (saline solution), topical application of the green and red propolis extract for 15 days. After this period the animals were sacrificed and the blood was used for we evaluated of the biochemical parameters. Results: It was found that of green and red propolis extract significantly decreased of triglycerides, glucose and cholesterol levels against control group. Conclusions: These results indicated that propolis may have roles in the carbohydrates and lipids metabolism. Other parameters are being evaluated, including, aminotransferases, urea, albumin and uric acid. Supported by: Fapeal



67

CODE 62 EVALUATION CYTOTOXIC IN VITRO OF NEW COMPOUND LPSF/MS-

02 Carvalho, M. S.1; Pinto, M. N. S.2*; Lima, M. C. A.3; Galdino, S. L.3; Pitta, I. R.3; Pessoa, C. Ó.4 ; Moraes, M. O.4

¹ Departamento de Biofísica e Farmacologia; Centro de Biociências, UFRN. Av. Salgado Filho, s/n Campus Natal, RN ² Departamento de Farmácia da UFRN, R. Gal. Gustavo Cordeiro de Farias, S/N – Petrópolis. Natal/RN ³ Departamento de Antibióticos da UFPE, Av. Prof. Moraes rego, Cidade Universitária,1235, Recife, PE 4 Laboratório de Oncologia Experimental da UFC, Rua Coronel Nunes de Melo, 1127, Fortaleza, CE Corresponding author: [email protected] Key-words: Hydantoins, imidazolidine, cytotoxicity Introduction: The chemistry and properties of hydantoins and their derivatives have been investigated for more than 140years. The hydantoin moiety represents an important pharmacophore, which is present in various biologically active compounds. Objectives: The compound LPSF/MS-02 [3-(4-methyl-benzyl)-5-(4-chloro-benzylidene)-imidazolidine-2,4-dione was synthesized and tested on the human cancer cell lines: HL-60 (promyelocytic leukemia), MDAMB-435 (melanoma - human), HCT-8 (colon - human) and SF-295 (glioblastoma - human). Materials and Methods: For comparative purposes we used an anticancer drug used in the clinic, doxorubicin (Dox). The IC50 values were calculated by exposure of the compounds tested in human tumor cell lines after 72 h of incubation, the MTT test. The compounds were serially diluted in RPMI to obtain the final concentrations (0,19-25 µg/mL) and added in 96-well plates (100μL/poço). Results and Discussion: The plates were read on spectrophotometer plate at a wavelength of 595nm. Active were considered those that showed IC50 < 4 μg/mL. LPSF/MS-02 was more selective for the melanoma cell line (MDAMB-435) with IC50 equal to 0.63 μg/mL, with a cytotoxic dose very close to the standard dose of the anticancer doxorubicin (0,48 μg/mL). Statistical analysis was performed according to their means and their standard errors and their deviations were made from non-linear regression using GraphPad Prism (version 5). Conclusions: Compound LPSF/MS-02 was synthesized in satisfactory yield and characterized spectroscopically and showed cytotoxic potential on human cell lines, which showed selectivity for lineage MDAMB-435, with a cytotoxic dose very close to the standard dose of the anticancer doxorubicin (0,48 μg/mL).



68

CODE 63 DETERMINATION OF PROTEIN CONTENT OF Zizyphus joazeiro.

Campos, R.S.1; Pinto, Y.S.1; Araújo, N.K.2; Pagnoncelli, M.G.B.1; Assis, C.F.1

1 Laboratório de Bromatologia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte 2 Laboratório de Engenharia Bioquímica, Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte Introduction: The Zizyphus joazeiro is widely distributed throughout the Brasilian Northest, its leaves and fruits are valuable food source for humans and animals. Objectives: The aim of this work was evaluate two ex c s (e h no c nd queous) us ng d ffe en me hods of p o e n’s determination. Materials and Methods: Ziziphus joazeiro fruits were collected in Macaíba city at Escola Agrícola de Jundiaí region of Rio G nde do No e S e, B z , ude 9º27’S nd ong ude 35º27’W of Greenwich, between the months of May and June, 2011.Than, were made aqueous and ethanol pulp and seed for protein quantify by spectroptotometer and content of nitrogen. The three methods spectroptotometer were used: BCA, Lowry and Biureto methods and Kjeldahl determination. Results and Discussion: The content of protein using the aqueous extract was higher than ethanolic extract; this confirms the higher solubility of proteins in water than organic solvent. According to the spectrophometric methods used in this work the most sensitive was BCA (Bicinchoninic Acid) methods. The pulp presented 12,5g protein/100 g sample, while seed 3,02 g protein /100 g sample. The Kjeldahl method present results of 6,66 g protein/100 pulp and 2,91 g protein/100 g seed. In comparison to the absolute values determined by Kjeldahl method, BCA remained the most reliable protein assay when quantifying protein of seed and pulp. Conclusion: According to our results we can conclude that the pulp has more protein and that the BCA had a higher sensibility in comparision of the others methods.



69

CODE 64 COMPARISON OF LABORATORY CHARACTERISTICS OF PATIENTS

WITH SICKLE CELL DISEASE Fernandes, T.A.A.M.1,5; Bezerra, C.M.2; Cabral, C.H.K.3; Leitão, R.L.T.S.3; Nascimento, A.N.C.4; Lopes, D.R.4; Sonati, M.F.5; Medeiros, T.M.D.4

1 Departamento de Ciências Biomédicas, Universidade do Estado do Rio Grande do Norte (UERN)

2 Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte (UFRN)

3 Hemocentro Dalton Barbosa Cunha (HEMONORTE)

4 Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN)

5 Laboratório de Micologia, Natal-RN

6 Departamento de Patologia Clínica, Universidade de Campinas (UNICAMP) Corresponding author: [email protected] Introduction: Sickle cell disease (SCD) is a constellation of similar disorders, all of which have at least 50% HbS in the blood. Homozygotes for the HbS gene are said to have sickle cell anemia; common compound

heterozygous forms of disease include HbSC disease and HbS- thalassemia. The phenotype are highly variable among theses patients. Objective: Compare the laboratory characteristics of patients with different genotypes of sickle cell disease. Materials and methods: We analyzed 44

patients (25 SS, 12 SC and 7 S-) attended at HEMONORTE, Natal-RN. The patients had not received blood transfusions in the preceding 3 months nor had taken hydroxyurea during the last 12 months. They were stratified by age group and we analyzed the following laboratorial parameters: erythrogram (RBC, HGB, HCT, MCV, MCH, MCHC, RDW), reticulocyte count, Hb A2 and Hb Fetal, leukocyte count, bilirubin (total, direct and indirect), creatinine, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and ferritin. Results and Discussion: The levels of RBC, HGB and HCT were

significantly lower in patients SS, and VCM and HCM were lower in S- patients. Besides, we found higher levels of reticulocyte count, Hb F and LDH in patients SS, when compared to SC. These results can be attributed to the higher levels of hemolysis found in patients SS, and the higher level of HbF can be explained by our exclusion criteria. Conclusions: Patients with different genotypes of sickle cell disease present statistically significant differences in some hematological and biochemical parameters. Financial Support: CNPq / Brazil, UERN / Brazil



70

CODE 65.1 UV SPECTROPHOTOMETRIC PROFILE OF CEFAZOLIN SODIUM

Pedroso, T. M.1*; Salgado H.R.N.1

1 Departamento de Fármacos e Medicamentos - Controle de Qualidade, FCF, UNESP, Araraquara, SP, Brasil Corresponding author: [email protected] Introduction: Cefazolin sodium (CFZ) is an antimicrobial agent β-lactam parenteral drug belonging to first generation cephalosporins. Its use in clinical practice stands out for its effectiveness and therapeutic agent as in surgical prophylaxis. Objective: This work proposes quantitative tests for the inclusion of cefazolin sodium in the Brazilian Pharmacopoeia. Material and Method: Spectrophotometer UV Shimadzu, quartz cells, aqueous solutions of CFZ at concentrations of 8, 12, 16, 20, 24 and 28 µg/mL. A standard calibration curve of CFZ was constructed by plotting absorbance versus concentration. The dilutions were analyzed individually in the UV and using their respective absorbance values an absorption curve was constructed. Results and Discussion: In the spectrophotometer, the absorption was obtained at 271 nm. The linearity of the method was proven by the correlation coefficients that were 0.9999 for standard reference and sample. The content of CFZ in the samples analyzed, 98.777%, is consistent with the official compendium from 95.0 to 102.0%. The accuracy of the method was proven by the recovery test, with an average of 100.56%. The robustness was assessed at 2 wavelengths, above and below 271 nm. Conclusions: The CFZ showed a linear relation between absorbance and concentration in wavelength. Thus, the results parameters demonstrated that the spectrophotometric method could be applied for the analysis of the pharmaceutical formulations assuring the quality and efficacy of the CFZ under investigation. Financial support: FAPESP, CAPES, PADC-FCF and ABL – Pharmaceutical industry.



71

CODE 65.2 A NEW, SAFE AND EASY METHOD OF THIN-LAYER

CHROMATOGRAPHY FOR CEFAZOLIN SODIUM IDENTIFICATION. Pedroso, T. M.1*; Salgado H.R.N.1

1 Departamento de Fármacos e Medicamentos - Controle de Qualidade, FCF, UNESP, Araraquara, SP, Brasil Corresponding author: [email protected] Introduction: Cefazolin sodium is an antimicrobial agent β-lactam parenteral drug belonging to first generation cephalosporins. The Thin-Layer Chromatography (TLC) is a common method fo d ug’s den f c on, because it is simple, fast, visual and relatively inexpensive. TLC is based on differential migration of components over a thin layer of adsorbent material retained on a flat surface. Objective: This research aims to develop an easy, safe and economical TLC method for cefazolin sodium identification, which can be used in the routine analysis of quality control. Material and Method: The mobile phases tested in this work were: methanol; absolute ethyl alcohol: water (80:20, v/v). The stationary phase used was silica gel impregnated in aluminum. The standard and sample solutions of cefazolin sodium were prepared in purified water at concentration of 10 mg/mL (w/v). It was applied 5 µL of both solutions on the plate. After the mobile ph se’s e u on, he spo s we e de ec ed by exposing the plate at UV chamber (365 nm) and/or iodine vapor until they appear. Results and Discussion: The chromatogram showed clearly two separated spots. The spot in the chromatogram obtained with the test solution was similar in position, color and size to the spot in the chromatogram obtained with reference solution. Conclusions: The TLC method proposed in this study is easy, safe for the operator, economical and can be used for cefazolin sodium identification, in the routine analysis of quality control.



72

CODE 66 VOLTAGE-OPERATED POTASSIUM CHANNELS ARE INVOLVED IN (-

)-BORNEOL-INDUCED VASORELAXANT RESPONSE ON RAT MESENTERIC ARTERY Silva-Filho, J. C.1; Oliveira, N. N. P. M.1; Ferreira-Filho, E. S.1; Sabino, C. K. B.1; Mendes, M. B.1; Arcanjo, D. D. R.1; Quintans-Junior, L. J.2; Santos, M. R. V.2; Oliveira, R. C. M.1; Oliveira, A. P.1 1 Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí (UFPI). 2 Departamento de Fisiologia, Universidade Federal de Sergipe (UFS). Corresponding author: [email protected] Introduction: The monoterpene (-)-borneol is found in essential oils of several medicinal plants. Previously, (-)-borneol showed analgesic, anti-inflammatory, antioxidant, vasorelaxant and anti-hypertensive properties. Objective: Evaluate the potassium channels involvement in (-)-borneol-induced vasorelaxant effect on rat superior mesenteric artery. Materials and Methods: Endothelium-intact and –denuded superior mesenteric artery rings (1-3 mm) from male Wistar rats were kept in Tyrode (pH 7.4; 37ºC), aerated with 95% O2 and 5% CO2 suspended by cotton threads and attached to force transducers coupled to a data acquisition system (AVS Projetos, Brazil) for registration of isometric tension. After a stabilization (1h / 0.75 g), rings were contracted with phenylephrine (10-5 M) and (-)-borneol (10-9 - 10-3 M) was added on contraction tonic phase. Potassium channels involvement were evaluated by tetraethylammonium (3 mM), 4-aminopyridine (1 mM) or glibenclamide (10 µM) pretreatment. Values were expressed as mean ± S.E.M. and results were considered significant when *p < 0,05. Results: The cumulative administration of (-)-borneol (10-9 - 10-3

M) induced a concentration-dependent vasorelaxant effect and independent of vascular endothelium (pD2 = 4.86 ± 0.18; 4.92 ± 0.14, endothelium-intact and -denuded, respectively; n=5). In endothelium-denuded rings, (-)-borneol-induced vasorelaxant response was attenuated after tetraethylammonium (pD2 = 4.38 ± 0.10*) or 4-aminopyridine (pD2 = 4.41 ± 0.08*) pretreatment, but not in glibenclamide pretreatment (pD2 = 4.86 ± 0.12). Conclusion: The monoterpene (-)-Borneol promoted a endothelium-independent vasorelaxant effect on rat superior mesenteric artery, and voltage-operated potassium channels are probably underlying this effect. Financial support from UFPI, UFS and CAPES/Brazil.



73

CODE 67 PREPARATION OF NANOSTRUCTURED SYSTEMS SUNSCREENS

ORGANIC – INORGANIC Rodrigues, K.1,2; Silva, T. A.3; Grillo, L. A. M.4; Dornelas, C. B.3; Magalhães, N. S.1,2

1 Departamento de Farmácia, Universidade Federal de Pernambuco. 2 Laboratório de Imunopatologia Keizo-Asami (LIKA), Bioquímica, Universidade Federal de Pernambuco Universidade Federal de Pernambuco. 3 Laboratório de Tecnologia e Controle de Medicamentos, Escola de Enfermagem e Farmácia – Curso de Farmácia, Universidade Federal de Alagoas (UFAL). 4 Laboratório de Biociências Farmacêuticas, Escola de Enfermagem e Farmácia – Curso de Farmácia, Universidade Federal de Alagoas (UFAL). Corresponding author: [email protected] Introduction: The improvement of research on products that block the various effects of ultraviolet radiation incident on the human body became the use of sunscreen indispensable. Several studies have demonstrated the necessity of better understanding new and traditional molecules used as sunscreens, as the use of some organic sunscreens can cause undesirable reactions such as photoisomerization and photosensitization, with a reduction of action, and potential systemic absorption. To overcome these limitations, it is necessary to develop safe and effective sunscreen preparations. Objective: The aim of this work was to prepare nanostructured systems sunscreens containing an organic sunscreen liquid-oil and an inorganic filter with lamellar silicate. Materials and Methods: The nanosystems were prepared by the solvent method, and the suspension obtained was submitted centrifugation and evaporation at reduced pressure to remove the solvent. Results and Discussions: After the rotaevaporation the nanosystems maintained the oily appearance but those obtained by centrifugation presented powder aspect. The result suggests that organic filter was intercalated between the silicate lamellae. Through spectrophotometry was possible to indirectly quantify the yield of reaction products obtained by centrifugation, which reached 95.36 ± 1.58%, significant result compared to others nanosystems that can carry drugs. Conclusions: As perspective, it is expected to analyze the possibly reduction of the adverse effects of the organic sunscreen, supposedly achieved by the formation of intercalated type structure, as well as ratify the synergism of the filters on the increase in sun protection factor. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil.



74

CODE 68 ANTIBACTERIAL EVALUATION OF NEW DERIVATIVES OF

THIOPHENE Xavier, L. A.1; Araújo, G. M. 1; Medeiros, R. G. L.1; Pinto, A. C. S.3; Mendonça Junior, F. J. B.4; Lima, M. C. A.4; Galdino, S. L.4; Pitta, I. R.4; Moura, R. O.5; Carvalho, M. S.2; Andrade, V. S.1 rade 1 Laboratório de Micologia Médica e Ambiental (LAMEA), Departamento de Microbiologia e Parasitologia, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN). ² Laboratório de Farmacologia, Departamento de Biofísica e Farmacologia, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN). ³ Curso de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN). 4 Laboratório de Planejamento e Síntese de Fármacos, Departamento de Antibióticos da Universidade Federal de Pernambuco (UFPE). 5 Laboratório de Síntese e Vetorização de Moléculas, CCBSA, Universidade Estadual da Paraíba (UEPB). Corresponding author: [email protected] Introduction: One structural characteristic present in several biologically active compounds, is the presence of the thiophene ring. Compounds with this feature present biological properties such as anti-inflammatory, antiviral and antifungal activities, making this a very attractive structure for medicinal chemists. Given the importance of the thiophene ring indicated in the literature, we pursued new thiophene derivative bioactive compounds like potential antibacterial activity. Objective: Evaluate the antibacterial potential in vitro thiazolidine analogs structurally modified. Materials and Methods: Filter paper discs were impregnated with samples (1mg/mL) and distributed on the surface of Mueller-Hinton agar previously inoculated with bacteria suspension. The strains tested were Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, were positives controls, respectively, oxacillin and norfloxacin (incubated at 36ºC for 24 hours). The determination of minimum inhibitory concentration was performed from the broth microdilution using gentamicin (10µg/mL) as positive control and for both tests: distilled water, 5% DMSO and 5% tween as a negative control. Results and Discussion: Among the series two compounds showed activity against a strain of S. aureus. The formation of small inhibitory zone observed in disk diffusion test and the color displayed in the development of microdilution suggest a possible bacteriostatic action, to be confirmed in bioassays currently in progress. These compounds deserve further structural investigations that may contribute to the enhancement of antimicrobial activity, considering the limitation of adaptive mechanisms of resistance by bacteria and their cytotoxicity. Conclusions: The constant search for new bioactive compounds is the first step in the development of new drugs as an alternative to the treatment of infections caused by resistant microorganisms. Financial support from CAPES, UFRN (PROPESQ-REUNI) /Brazil.



75

CODE 69 THEORETICAL AND PRACTICAL HANDBOOK: A TOOL FOR THE

TRAINEESHIP LIVE PHARMACIES. Moura, C. A.1; Bandeira, M. A. M.1

1 Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Ceará (UFC). Corresponding author: [email protected] Introduction: Setting the list of medicinal plants to be used in Herbal Medicine in Public Health, The State Board of Phytotherapy, based on the UFC Living Pharmacies/LP Project, took into account the epidemiological profile of the population of Ceará and the efficacy of treatment, then thirty medicinal plants selected were scientifically validated. The graduation students of the Course of Pharmacy UFC perform curricular traineeship on the LP that as one of the focus identifying these plants included in that Project, so for these students recognize these medicinal plants in herbal medicine services are required research sources reliable and easily accessible. Objective: Develop a Theoretical and Practical Handbook about characterization of medicinal plants to assist in traineeship in LP. Materials and Methods: We carried out several literature researches on methods of macro and microscopic identification of the component parts of plants. From these data, we prepared a Handbook adapted to the thirty medicinal plants consisting of on the theoretical characterization of leaves, bark and underground organs. Compounding the album yet, put up boards with illustrative drawings and tables that enable gather therapeutic information, botanical identification and major macro and microscopic features of these parts, with room for schematic drawings by the trainees. Results and Discussion:Using the Handbook, during the traineeship is expected the consolidation of knowledge acquired by trainees. Conclusions:The development of this Handbook, in addition to improving the fixation of the knowledge acquired during the traineeship, can be used by students as a source of research on plants of LP.



76

CODE 70 DEVELOPMENT OF STRATEGIES OF MOLECULAR DIAGNOSTIC

APPLIED TO DYSLIPIDEMIA Osorio, E. D. S. S.¹; Santos, A. B.¹; Bastos, R. C.¹; Andre, N. V. C.¹; Azevedo, N. L.¹; Rezende, A. A.¹; Luchessi, A. D.¹; Silbiger, V. N.¹ ¹ Laboratório de Biologia Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] This study select candidate genes and SNPs to establish molecular strategies to genotyping and gene expression associated with cardiovascular diseases (CDs) and dyslipidemia. The selection candidate genes were performed by research in articles published (2008-2011) in PubMed/NCBI followed by gene and diseases interaction analysis using the Ingenuity Pathway Analysis. Candidate SNPs were select using the dbSNP/NCBI. The primers for genotyping strategies were determined using the Primer Express 3.0 to the TaqMan® system, Primer BLAST/NCBI and Web Site NEBcutter V2.0 to the PCR-RFLP and PyroMark® Assay 2.0 to the pyrosequencing of according with this priority order. Firstly 35 were selection as candidate genes of dyslipidemia. Among of them 14, 4 and 5 genes presented direct, indirect and without relationship with dyslipidemia, respectively. Considering the previously association with CDs at the end 11 genes were selected, 6 to elaborate the genotyping strategies (CHI3L1; FABP2; INSIG1; NPC1L1; PCSK9 and TRIB1) and another 5 genes for gene expression (APOA1; APOB; APOC3; APOE and LDLR). It was possible to select 4 SNPs of gene CHI3L1, 8 of FABP2, 10 of INSIG1, 6 of NPC1L1, 1 of PCSK9 and 10 of TRIB1. Fifty-two percent of SNPs can be determinate by TaqMan® real time PCR, 2% by PCR-RFLP and 23% by pyrosequencing. Also it was possible select primers and probes of gene expression by TaqMan® real time PCR. This study created one list of candidate genes, SNPs and mRNAs using tools of interaction between genes and diseases providing the determination of strategies of genotyping and gene expression.



77

CODE 71 EVALUATION OF THE ROLE OF THE NITRIC OXIDE IN

GASTROPROTECTIVE ACTIVITY OF PARTITION FRACTIONS OF Neoglaziovia variegata Mez. (BROMELIACEAE) IN MODEL OF ULCERS INDUCED BY ETHANOL Machado, F. D. F.1; Bezerra, M. A.1; Diniz, J. A.1; Viana, A. F. S. C.1; Oliveira, I. S.1; Lima, J. T.2; Almeida, J. R. G. S.2; Oliveira, F. A.1; Oliveira R. C. M.1 1 Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí (UFPI). 2 Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco (UNIVASF). Corresponding author: [email protected] Introduction: Previous studies have shown that partition fractions of ethanol extract of Neoglaziovia variegata Mez (NV-EtOH), presents cytoprotective activity in animal models of ulcer induced by ethanol and ischemia/reperfusion.Objective: To evaluate the involvement of nitric oxide (NO) in the gastroprotection of ethyl-acetate (NV-AcOEt), hexane (NV-Hex) and chloroform (NV-CHCl3) fractions in models of gastric ulcer induced by ethanol in mice. Materials and Methods: Swiss albino mice (25-30 g, n = 10), fasting 18 h, were treated intraperitoneally with vehicle (0.9 % saline/10 mL/kg) or L-NG-nitro arginina (L-NOARG 70 mg/kg). After 30 min, the animals received NV-AcOEt, NV-Hex, NV-CHCl3 at dose 100 mg/kg p.o, vehicle (0.9 % saline p.o) or L-arginina (600 mg/kg i.p.). 1 h after treatment with NV-AcOEt, NV-Hex, NV-CHCl3 or vehicle and 30 min later administration of L-arginina each animal was given orally 0.2 ml of ethanol. It were euthanized 30 min later. After euthanasia all the stomachs removed, opened and area of injury measured by planimetery (mm2). All animal experiments protocols were approved by Ethics Committee of the Federal University of Piauí (Nº 076/10). The significance level was evaluated for values of *p < 0.05 (ANOVA one way). Results and discussion: L-NOARG reversed the protection gastric mucosa presented by NV-AcOEt (1.8 ± 0.25 *) to (15.9 ± 3.02), NV-Hex (3.5 ± 0.32 ***) to (23.86 ± 3.16), however not inhibited the gastroprotector effect of NV-CHCl3 significantly. Conclusions: The results suggesting a possible participation of NO in the gastrprotective activity NV-AcOEt and NV-Hex. Financial support: UFPI, CAPES, UNIVASF, FACEPE, CNPq/Brazil.



78

CODE 72 OPTIMIZATION OF AN EXTRACTION PROCEDURE FOR THE

BIOPOLYMER CHITIN De Souza, I.1; Santos, S. R. A.1; Pedrosa, M. F. F.1,2; Fonseca, J. L.3; Silva-Júnior, A. A.1,2 1 Undergraduate course of Pharmacy, Federal University of Rio Grande do Norte (UFRN). 2 Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte (UFRN). 3 Department of Chemistry, Federal University of Rio Grande do Norte (UFRN). Introduction: The biggest activity of shrimp farming in Brazil is located in Rio Grande do Norte. The waste from this production is generally discharged. However it is possible to extract chitin, a polymer widely used as raw material for adsorbents, chelating agents and for production of chitosan. Objectives: To optimize an extraction procedure for the biopolymer chitin using the shell waste. Materials and methods: The extraction procedure was conducted in laboratory scale and optimized by varying the concentration of HCl (2.5% w/ v, 5.0% w/ v, 7.5% w/v 10% w/v). The physicochemical characterization included thermal analysis (thermogravimetry - TG), Infrared Spectroscopy (IR), scanning electron microscopy (SEM) and X-ray diffraction (XRD).Results and discussion: The TG analysis showed that chitins treated with different acid solutions presented different mineral residues (22.49, 16.73, 10.19 and 12.99%) for samples treated with 2.5, 5.0, 7.5 and 10.0% respectively. The IR spectrum showed the characteristic stretching bonds at 3444cm-1 (OH axial stretching OH); 3270cm-1 e 3107cm-1 (NH axial stretching)of chitin. The X-ray analysis confirms the crystallinity of the compound extracted. SEM micrographs chitin showed homogeneous, compact and smooth surface particles.Conclusion: The experimental results confirmed that the s mp e ex c ed w s α-chitin. XRD patterns showed the characteristic crystalline domains. TG curves showed that the mineral residues decreased with increasing solvent concentration, and the better acid solution was with HCl 7.5% w/v. The optimization of extraction procedure was effective showing that variations in the solvent concentration can interfere with the purity of this biopolymer.



79

CODE 73.1 USE OF POTENTIALLY INAPPROPRIATE MEDICATIONS AND THE

RISK OF POSSIBLE DRUG INTERACTIONS BETWEEN INSTITUTIONALIZED FRAIL ELDERLY IN A CITY IN THE ZONA DA MATA MINEIRA Zimmermann-Franco, D. C. 1,2; Fochat, R. C.1,2; Horsth, R. B. O.1; Sette, M. S.3; Raposo, N. R. B.1,2; Chicourel, E. L.1,2

1 Núcleo de Identificação e Quantificação Analítica, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF). 2 Núcleo de Pesquisa e Inovação em Ciências da Saúde, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF). 3 Faculdade de Medicina, Universidade Federal de Juiz de Fora (UFJF). Corresponding author: [email protected] Introduction: Drugs have great importance in health care for the elderly and are more consumed with advancing age. However, some drugs are considered potentially unsuitable for the elderly because they are not effective and/or are an unnecessarily high risk. The use of multiple medications also should be supervised because it increases the risk of drug-related problems. Objectives: To evaluate the presence of potentially inappropriate medications and possible drug interactions between institutionalized frail elderly in Juiz de Fora (MG). Materials and Methods: It was consulted personal records and prescriptions of 122 individuals. The drugs were classified according to Anatomical Therapeutic Chemical Classification System and the Beer-Fick criterion to identify those potentially inadequate. Possible interactions were established with Micromedex. Results and Discussion: The results showed that 68.0% of the subjects were women, 55.7% were 80 years or older and 67.2% of them were polymedicated. Diseases of the circulatory system (27.0%) were the most prevalent. There were 219 possible interactions (87.7% severity moderate/severe and 70.8% of scientific evidence good/excellent) involving 75 elderly (61.5%), and the antiepileptics, psycholeptics, diuretics, agents that act in renin-angiotensin system were the main responsible for possible interactions. It was observed that 44 elderly (36.0%) used 58 potentially inappropriate drugs (8.2%), 87.9% could have serious implications. Conclusion: These findings indicate some gaps in prescription and highlight the need to review the drug regimens, aiming towards their rational, safe and effective use. Financial support: CNPq; CAPES; Propesq/UFJF.




80

CODE 73.2 ANTIFUNGAL ACTIVITY OF OLEORESIN EXTRACTED FROM

Copaifera langsdorffii Desf AGAINST DERMATOPHYTES FUNGI Zimmermann-Franco, D. C.1; Baptista, E. B.1; Chaves, M. G. A.2; Raposo, N. R. B.1,3 1 Núcleo de Identificação e Quantificação Analítica, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF). 2 Faculdade de Odontologia, Universidade Federal de Juiz de Fora (UFJF). 3 Núcleo de Pesquisa e Inovação em Ciências da Saúde, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF). Corresponding author: [email protected] Introduction: Essential oils and resin stand out as the main elements associated with the plant's defense against bacteria and fungi. Therefore, it have been demonstrated a promising source of compounds with antimicrobial activity. The cutaneous mycoses are diseases worldwide, which consume millions of dollars with treatment costs each year. The oleoresin extracted from Copaifera langsdorffii Desf. is widely used in Brazilian popular medicine under the name copaiba, predominantly as a healing, antiseptic and antiinflammatory agent. Objective: To evaluate the antifungical activity of oleoresin extracted from copaiba against species of two dermatophytes fungi: Microsporum canis and Trichophyton mentagrophytes. Material and Methods: The qualitative antifungal assays were performed using fragments (2 mm) of Microsporum canis (ATCC 32903) and Tricophyton mentagrophytes (ATCC 11481). The fragments were inoculated on Sabouraud Dextrose agar previously incorporated with oleoresin of copaiba (1000 µg. mL-1) and the antifungal activity was determined by total growth inhibition. The minimal inhibitory concentration (MIC) was performed, according to recommendations of the Clinical and Laboratory Standards Institute (M-38 A2), with adaptations. Results and Discussion: Qualitative results showed that the copaiba oleoresin presented activity against M. canis e T. mentagrophytes. The quantitative evaluation showed that 125 µg.mL-1 oleoresin inhibited the growth of M. canis and 8 µg.mL-1 inhibited the growth of T. mentagrophytes. Conclusion: These findings provide basic information for the use of this oleoresin extract from copaiba in fungical infectious, but further studies are required. Financial support: Propesq/UFJF.



81

CODE 74 EVALUATION OF ACCELERATED STABILITY OF SOYBEAN O/W

EMULSION CONTAINING ETHANOL AND BENZYL ALCOHOL AS COSURFACTANTS De Araujo, M. M.1; De Souza, I.2; Streck, L.1; Santos, S. R. A.2; Oliveira, A.G.3; Silva-Júnior, A. A.1 1 Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte (UFRN). 2 Undergraduate Course of Pharmacy, Federal University of Rio Grande do Norte (UFRN). 3 Graduate Program in Pharmaceutical Sciences, Pharmaceutical Sciences Scholl of Araraquara-SP, Unesp-SP. Corresponding author: [email protected] Introduction: The suitable emulsions (EMs) should present a defined period and pre-determined of physical and chemical stability depending on the application. Objective: This study evaluates different concentrations (5-15%v/v) of ethanol and benzyl alcohol as cosurfactant associated with a mixture of tween 80 and span 80 (HLB=11) in physicochemical stability of a soybean w/o emulsions. Materials and methods: The analytical methodology was validated with success. The stability study of different formulations was conducted during 30 days, in different conditions every day, such as 24 hours refrigeration (RE) (4 ± 2 °C) 24 hours ambient temperature (AM) (25 ± 2 °C) and 24 hours warming (AQ) (45 ± 2 °C). Different parameters such as creaming index, pH, conductivity and turbidity of samples were recorded at intervals of 1, 7, 15 and 30 days. Results and discussion: The ethanol did not lead to statistical differences on the physicochemical stability parameters of the emulsions. However, the benzyl alcohol led to decreasing the stability of the emulsions. Conclusion: The different investigated concentrations of ethanol and benzyl alcohol did not lead to increment in the physicochemical stability. On the contrary, benzyl alcohol lead to unstable emulsions. Financial support: CAPES and CNPq



82

CODE 75 EVALUATION OF MORPHOGENESIS AND PROTEINASE ACTIVITY OF

CLINICAL ISOLATES OF C. albicans OBTAINED FROM PATIENTS WITH VULVOVAGINAL CANDIDIASIS Medeiros, M. A. P.1; Melo, A. P. V.1; Pereira, R. M.1; Milan, E. P.2; Chaves, G. M.1

1 Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN). 2 Departamento de Infectologia, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected]

Introduction: C. albicans is the most virulent species of the genus Candida. This fact can be explained by the presence of a group of virulence attributes which contribute for its pathogenicity. Vulvovaginal candidiasis (VVC) is one of the most common causes of vaginitis and the majority of the cases (80 to 90%) are due to C. albicans. Several virulence attributes are proposed in VVC, but a few of them have been studied. Objective: The present study aims to evaluate bud-to-hypha transition (morphogenesis) and proteinase activity of clinical isolates of C. albicans sequentially obtained from the anus and vagina of patients with VVC by using phenotypic methods. Materials and Methods: We evaluated 24 strains of C. albicans belonging to our yeast culture collection. Hypha formation was induced by incubating Candida cells in bovine fetal serum diluted at 20% in broth YPD, and the morphological index determined by optical microscopy after 3 h incubation. We used the YCB + BSA medium to induce proteinase production, and determined the proteolytic activity as the amount of degraded albumin as a substrate (OD 280 nm). Results and Discussion: The morphological index of the strains ranged from 1,8 to 3,7, while the proteinase activity ranged from 0,03 to 0,09. Conclusions: The vaginal isolates showed in general higher virulence properties, or similar levels to the corresponding anal isolate. While sequential isolates showed an increase of proteinase activity followed by a reduction of the enzyme levels, they had a trend to form filamentous morphologies as the infection succeeded. Financial support from FAPERN/Brazil.



83

CODE 76 DETERMINATION OF LODENAFIL CARBONATE USING CAPILLARY

ZONE ELECTROPHORESIS METHOD Codevilla C.F.1,*; Ferreira P.C.L.1; Sangoi M.S.1; Fröehlich P.E.1; Bergold A.M.1

1Laboratório de Produção de Padrões Secundários (LAPPS), Faculdade de Farmácia, UFRGS. E-mail: [email protected] Introduction: Lodenafil carbonate (LOC) is a phosphodiesterase type 5 inhibitors that show safety and efficacy in treating erectile dysfunction. The literature shows few quantitation studies of LOC in tablets and there is no described method for determination of LOC using capillary zone electrophoresis (CZE). Objective: The aim of this study was the development, optimization and validation of a CZE method for the determination of LOC in tablets, establishing comparison with the validated HPLC and UV spectrophotometric methods. Materials and Methods: CZE experiments were performed on an Agilent 3DCE apparatus. All experiments were carried out on an uncoated fused-silica capillary with 40 cm effective length, at 32.5 ºC. The applied voltage was 15 kV, detection at 214 nm and injection volume of 5 s at 50 mBar. The electrolyte used was borate buffer 50 mM, pH 10. The optimized method was validated according to ICH. Results and Discussion: The effects of background electrolyte concentration, pH, capillary temperature and voltage were investigated by means of response surface methodology. The method shows to have specificity, linearity, accuracy and precision. The robustness was studied using a 2-Level 24-1 fractional factorial design. Results obtained by CZE method were compared statistically by ANOVA with HPLC and UV spectrophotometric methods previously validated and showed no significant difference between the techniques. Conclusions: A simple CZE method was successfully developed for the determination of LOC in tablets. The proposed method can be used as an alternative for the routine quality control of LOC in pharmaceutical formulations. Financial support from CNPq/Brazil.



84

CODE 77 ANALYSIS OF EPIDEMIOLOGICAL SITUATION OF LEPROSY IN

CHILDREN UNDER 15 YEARS OLD IN FORTALEZA IN THE PERIOD OF 2001 TO 2010. Costa, M. A. R.¹*; Oliveira, Y. S.¹; Thé, M. F. D. S.¹; Carvalho, M. M. F.¹; Costa, W. S.¹; Assis, C. N.¹; Amaral, H. E. G.² ¹ Department of Pharmacy, Federal University of Ceará, Fortaleza/CE-Brazil ²Cell of Epidemiological Surveillance of Fortaleza (CEVEPI). *[email protected] Introduction: Leprosy is a chronic infectious disease, transmissible, has a slow evolution and has as etiologic agent Mycobacterium leprae. The detection of cases in minors with less than 15 years demonstrates that early exposure of this population to the bacillus, and has been used as an epidemiological indicator for signaling the dynamics of recent transmission and the magnitude of the problem. Objective: Analyze the situation of leprosy in children under 15 years old in the city of Fortaleza in the period 2001 to 2010. Materials and Methods: A retrospective descriptive study based on information generated by the National Notifiable Diseases System, covering the cases of Leprosy occurred in children younger than 15 years in Fortaleza in the period 2001 to 2010. Results and Discussion: The analysis results show a ascending behavior in the rate of detection of the disease from 2001 to 2005. From 2006 to 2010 there was a slight decline and in 2008 was observed a greater number of cases reported (94). Despite this, during the whole period the number of cases was considered high, featuring a hyperendemic (1,11/10,000 inhabitants). It may be related to early exposure of these minors to cases not treated yet, to the delayed diagnosis and treatment, to the incorrect diagnosis caused by errors in the differential approach with other dermatological diseases. Conclusion: It is extremely important the participation of health teams in the early diagnosis of leprosy in children with less than 15 years, with the development of preventive actions of control.



85

CODE 78.1 IN VITRO INHIBITION OF CLINICAL Pseudomonas aeruginosa

ADHESION BY XYLITOL Sousa, L.P. 1; Silva, A.F. 1; Calil, N.O. 1; Suzuki, E.Y. 1*; Silva, S.S.2; Raposo, N.R.B. 1,3 1Núcleo de Identificação e Quantificação Analítica, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF). 2Departamento de Biotecnologia, Escola de Engenharia de Lorena – Universidade de São Paulo (USP). 3Núcleo de Pesquisa e Inovação em Ciências da Saúde, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF) Author for correspondence: [email protected] Introduction. In the scientific literature, there are some reports on the anti-adherent action of xylitol, a five-carbon polyalcohol, on many bacteria. A very promising approach is based on the awareness of the microorganisms adherence to several surfaces, and of the mechanisms which may prevent such colonization. Objective. The present study aimed at evaluating the antimicrobial and the anti-adherent activities of xylitol on a clinical strain of Pseudomonas aeruginosa, responsible for biofilms development on implanted devices and hospital equipment. Material and Methods. The test of antimicrobial activity was realized to determinate a minimum inhibitory concentration (MIC), using chloramphenicol as reference antibiotic. It was performed two parameters to evaluate the adhesion test: 1) number of colony-forming units (CFUs) released and 2) evaluation of slides using scanning electron microscopy (SEM). Results and Discussion. It was found that 0.5%, 2.5% and 5.0% concentrations of xylitol do not have antimicrobial activity on the strain tested. However, the inhibition of adherence, not dose-dependent, was demonstrated both by counting the released bacilli and by SEM microphotographs. For 0.5%, 2.5% and 5.0% concentrations of xylitol were released, respectively,46 ± 35 x 106; 15 ± 13 x 106 and 69 ± 1 x 105 UFCs. Xylitol was able to prevent the bacilli adherence on the slides evaluated by SEM, in all concentrations tested. These results corroborate with previous studies that have shown the use of 5.0% xylitol in the prevention of Pseudomonas aeruginosa biofilm development. Conclusion. Xylitol could be an appropriate biomolecule to prevent Pseudomonas aeruginosa biofilms. Financial support from CAPES/Brazil

http://mail.uol.com.br/[email protected]



86

CODE 78.2 ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL FROM Baccharis

trimera (Less) DC Suzuki, E.Y.1*; Chaves, M.G.A.M2,3; Raposo, N.R.B.1,2

1Núcleo de Identificação e Quantificação Analítica, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF) 2Núcleo de Pesquisa e Inovação em Ciências da Saúde, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF) 3Faculdade de Odontologia, Universidade Federal de Juiz de Fora (UFJF) *Author for correspondence: [email protected] Introduction: Baccharis trimera (Asteraceae) is a medicinal Brazilian plant well-known as "carqueja". Natural from tropical regions, it is traditionally used in folk medicine as anti-inflammatory, hypoglycemiant and for the treatment of digestive problems. Objectives. The present study aimed to investigate the antimicrobial activity against four microorganisms and to establish the minimum inhibitory concentration (MIC) of the essential oil obtained from Baccharis trimera. Material and methods. The microorganisms tested were Corynebacterium xerosis IAL105, Staphylococcus epidermidis ATCC12228, Proteus vulgaris ATCC13315 and Micrococcus luteus ATCC7468. Antimicrobial activity was determined using the broth microdilution method and the MIC was then established by the turbidity in the growth medium after 24hs of incubation. The oil was prepared at a concentration ranging from 62.5 to 1000 μg/ml. In addition, chloramphenicol (0.025 – 250 μg/ml) was used as reference antibiotic. Tests were carried out in triplicate. Results. Bactericidal activity of essential oil was observed against P. vulgaris (MIC=1000μg/ml) and bacteriostatic activity was observed against M. luteus and C. xerosis with the same MIC values (MIC=500μg/ml). Chloramphenicol demonstrated bactericidal activity against all tested microorganisms with MIC values of 25 μg/ml for S. epidermidis, P. vulgaris and M. luteus and 250μg/ml for C. xerosis. It also showed bacteriostatic activity against S. epidermidis, P. vulgaris and M. luteus with values of 2.5μg/ml and 25μg/ml for C. xerosis. Conclusion. These findings provide basic information for the use of this species in infectious processes, but further studies are required. Financial support from CAPES/Brazil

http://mail.uol.com.br/[email protected]



87

CODE 80 PHYSICAL-CHEMICAL COMPATIBILITY BETWEEN ATTAPULGITE,

RIFAMPICIN AND ISONIAZID Soares, D.S1, Fernandes, C. S.1, Raffin, F.1, Acchar2, W., Moura, T. M.1. 1Drug Development Laboratory, Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil 2Physical Properties of Ceramic Materials Laboratory Department of Physics, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil Corresponding author: [email protected] Introduction: According to composition, clays can be employed as pharmaceutical excipients. Previous studies showed that attapulgite, one type of clay from northeastern of Brazil, has potential for use as lubricant. To employ a compound as excipient, it is necessary to evaluate its compatibility with the drugs. Rifampicin and isoniazid are the main drugs used in the treatment of tuberculosis. Objective: Evaluate the physical-chemical compatibility between Attapulgite, Rifampicin and Isoniazid mixtures by differential scanning calorimetry (DSC). Materials and Methods: Physical mixtures were prepared in the different proportions of Rifampicin, Isoniazid and Attapulgite. To evaluate physical-chemical incompatibilities between drugs and clay, the DSC curves were obtained on a DSC-60 cell (Shimadzu) using aluminum crucibles with about 2 mg of sample, under dynamic N2 atmosphere (50 mL min-1) and heating rate of 10 °C min-1 between 25 to 400 °C. Results: On the DSC curve of rifampicin, there is an endothermic event due to melting (Tpeak = 181,7 °C). The second event is exothermic and is attributed to a recrystallization (Tpeak = 198,7 °C) and corresponds to conversion of polymorphic form II into polymorphic form I. On the DSC curve of isoniazid, it is observed an endothermic event due to melting (Tpeak = 172,9 °C). On the DSC curve of attapulgite, no transition it was observed. Concerning to the thermal behavior of the mixtures comparing to the drugs alone, no significant changes could be observed. Conclusion: According to DSC, attapulgite

presents no incompatibility with the tested drugs. Financial support: FAPERN, UFRN.



88

CODE 81 NEW METHOD TO PRODUCE XYLAN MICROPARTICLES USING

SODIUM TRIMETAPHOSPHATE AS CROSS-LINKING AGENT Costa, S. C.1; Lucena, C. A. A.1; Gomes, A. A. S.1; Marcelino, H. R.2; Souza, B. S.2; Gomes, M. C. S.2; Eleamen, G. R. A.1; Silva, A. E.2; Egito, E. S. T.2; Oliveira, E. E1. 1Laboratório de Síntese e Vetorização de Moléculas (LSVM), Univ. Estadual da Paraíba (UEPB) 2Laboratório de Sistemas Dispersos (LASID), Univ. Federal do Rio Grande do Norte (UFRN) Corresponding author: [email protected], Introduction: Xylan is the second most abundant biopolymer in the plant kingdom. It is the most common hemicellulose component in cell wall of agricultural crops such as straw, sorghum, sugar cane and corn cobs. Such polysaccharide can be hydrolyzed in colonic environment in human body. This fact enables the use of xylan to produce colon specific drug carriers. Objective: The aim of this work was to develop a new method to produce xylan microparticles by emulsion cross-linking method using sodium trimetaphosphate as cross-linking agent. Materials and Methods: An alkaline solution containing xylan and sodium trimetaphosphate was emulsified in 150 mL of liquid paraffin contain 7.5g of Span 80. The cross-linking reaction took place at 50ºC with a constant stirring speed of 800rpm. After 6h of reaction, the microparticles were isolate and washed with acetone, petroleum ether, deionized water and absolute alcohol by means of centrifugation. Finally, the xylan microparticles were dried under vacuum for 24h and kept in closed containers. Results and Discussion: The results showed that xylan microparticles presented suitable characteristics, with a good homogeneity. Regarding the visual aspect, the sample appeared to be a yellowish powder. Microscopy analysis showed that microparticles were spherical in shape and no agglomerates were formed with a mean particle size of 7.7 ± 0.17µm. Conclusions: The results showed that xylan microparticles produced with sodium trimetaphosphate can represent an eligible colon specific drug delivery system. Financial Support from PROPESQ/UEPB and CAPES/Brazil



89

CODE 82 SYNTHESIS OF TIN (IV) COMPLEXES WITH POTENTIAL

ANTIMICROBIAL ACTIVITY Guedes, J. S.¹; Barbosa, A. S. L.¹; Meneghetti, M. R.¹ ¹ Grupo de Catálise e Reatividade Química, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas (UFAL) Corresponding author: [email protected] Introduction. The discovery of cisplatin was a revolution in medicinal chemistry. The formation of drugs containing metal elements in their structures allows the development of a new series of synthetic drugs. One potential candidate to such compounds, are organometallic tin complexes. The association of tin compounds with bioactive ligands can be a suitable strategy to the development of hybrid drugs, i.e. at least two pharmacofore groups in the same drug.Objective. Synthesize of new tin organometallic compounds bearing ligands with potential pharmacological activity. Materials and Methods. The complexes n-Bu2SnCl2, Me3SnCl, Me2SnCl2, n-Bu3SnCl, and t-Bu2SnCl2 were used as tin source compounds, and the sodium salt of the nalidixic acid as bioactive ligand precursor. The complexes were prepared from the reaction of the tin source and the sodium salt of the nalidixic acid (Nal.Na) in toluene at refluxed conditions for 24 hours under argon atmosphere. The mixture is cooled down and chloroform is added, the solid is removed out and the solution is concentrated under high vacuum. The desired products are normally isolated as white solids. Yields around 80%. Results and Discussion. Until now, all complexes were characterized by 1H NMR and FTIR, giving strong evidences of the formation of the complexes n-Bu2Sn(Nal)2, n-Bu3SnNal, Me3SnNal, and t-Bu2Sn(Nal)2. In the 1H NMR can recognize signs of the ligand and the organotin used in the synthesis. The antibacterial activity of these complexes are under investigation. Conclusion. The methodology was simple and effective, the potential antibacterial activity is under investigation.



90

CODE 83 ADVERSE DRUG REACTIONS AS A CAUSE OF HOSPITAL

ADMISSION Medeiros, J. W. T.1; Oliveira, R. A.1; Pinto, C. O.1; Irineu, M. M.1; Barbosa, M. M. S.2; Brito, A. E.2

1Academic of Pharmacy, Hospital Universitário Onofre Lopes (HUOL), Universidade Federal do Rio Grande do Norte (UFRN)

2Pharmacist, Hospital Universitário Onofre Lopes (HUOL), Universidade Federal do Rio Grande do Norte (UFRN) Corresponding author: [email protected] Introduction: The pharmacovigilance has the function to search and monitor adverse drug reactions (ADRs), which according to World Health Organization (WHO) is "a response to a drug that is noxious and unintended and occurs at doses normally used in man for the prophylaxis, d gnos s o he py of d se se”. One c n es m e h 3-6% of all hospital admissions are caused by ADRs and only 10% are reported. Objective: This study aims to quantify the suspected ADRs that led to hospitalization at Hospital Universitário Onofre Lopes (HUOL). Methodology: The suspected ADR as a cause of admission was evaluated through an active search of medical records of patients newly admitted to the HUOL, a teaching hospital, tertiary, with 189 beds in the period from January to October 2011. Adverse reactions were classified according to causality and severity. Results: 6 suspected adverse reactions were reported as cause of hospitalization, of which one was classified as definite, 2 were probable and 3 were possible. As for the gravity, one was lethal, one was severe and 4 were moderate. Conclusion: One can observe that there is an underreporting due to lack of diagnosis of ADRs at the time of hospitalization and that the ADRs reported responsible by hospitalization were severe, moderate and lethal. This is the first study in institution that will provide the basis to encourage voluntary reporting by the physician at the time of admission. Financial support: unfunded.



91

CODE 84.1 ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS LEAF EXTRACT OF

Jatropha gossypiifolia L. IN CARRAGEENIN-INDUCED PAW EDEMA IN RATS Félix-Silva, J.1; Barbosa, L. M. Q.2; Gomes, J. A. S.1; Pinheiro, I. T. M. G.1; Silva-Júnior, A. A.1,2; Zucolotto, S. M.2; Fernandes-Pedrosa, M. F.1,2* 1Laboratório de Tecnologia & Biotecnologia Farmacêutica (TecBioFar), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN) 2Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN) *Corresponding author: [email protected] Introduction: Jatropha gossypiifolia L. (Euphorbiaceae), popularly known in Brazil as “p nhão- oxo”, s vege spec e used n fo k med c ne s n anti-inflammatory, antiophidic, antipyretic, and healing drug. It is related in the National List of Medicinal Plants of Interest to the Brazilian Public Health System (RENISUS) due its recognized therapeutic potential. Objective: Evaluate the systemic (oral route) anti-inflammatory activity of aqueous leaf extract of Jatropha gossypiifolia (JG) and characterize its preliminary phytochemical constitution. Materials and Methods: JG, prepared by infusion, was first characterized by phytochemical screening and thin layer chromatography. Then, it anti-inflammatory activity (50-200 mg/kg) was evaluated against carrageenin-induced paw edema in rats. Extracts were administered orally one hour before carrageenin injection (1 mg/paw). Results and Discussion: Were detected alkaloids, phenols, flavonoids, gums, resins, and sulfur compounds in JG, being flavonoids (isoorientin and vitexin) identified as the possible major substances. At 100 and 200 mg/kg doses, JG were effective in reducing carrageenin-induced paw edema (p<0.05), with intensity similar to standard anti-inflammatory drug indomethacin. The peak effect occurred around the third hour after injection of carrageenin, which corresponds to the period in which there is principally production of prostaglandins in this model, suggesting an inhibitory effect on cyclooxygenase action, in accordance with studies showing that flavonoids have important inhibitory effect on arachidonic acid metabolism. Conclusions: These results suggest, for the first time, the potential of aqueous leaf extract of Jatropha gossypiifolia as a source of molecules with anti-inflammatory activity, and seem to justify one of its main popular uses. Financial support: CNPq/Brazil.



92

CODE 84.2 ANTI-INFLAMMATORY ACTIVITY OF BULLFROG (Rana catesbeiana)

OIL IN CARRAGEENININDUCED PAW EDEMA IN RATS Barbosa, L. M. Q.1; Félix-Silva, J.2; Gomes, J. A. S.2; Pinheiro, I. T. M. G.2; Rocha, K. B. P.3; Fernandes-Pedrosa, M. F.1,2; Egito, E. S. T.1* 1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte (UFRN) 2Laboratório de Tecnologia & Biotecnologia Farmacêutica (TecBioFar), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN) 3Departamento de Patologia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN) *Corresponding author: [email protected] Introduction: Bullfrog oil (BFO) is a mixture of lipid compounds extracted from fat tissue from American bullfrog (Rana catesbeiana) legs. It is largely used in Brazilian folk medicine as a topic or oral drug for treatment of inflammation and others related diseases. Objective: Evaluate the systemic (oral route) anti-inflammatory activity of BFO in rats. Materials and Methods: The anti-inflammatory activity of BFO was evaluated against carrageenin-induced paw edema in Wistar rats. One hour before carrageenin injection (1 mg/paw), BFO was administered orally at 50, 100, and 200 mg/kg, and progression of inflammation was measured by 4 hou s. H s op ho og c n ys s of ’s p ws w s pe fo med n o de o investigate tissue changes after four hours of experiment. Results and Discussion: BFO, at all doses tested, was effective in reducing carrageenin-induced paw edema (p<0.05 compared to control group), in the same magnitude of indomethacin (standard anti-inflammatory drug). Histopathological analysis showed that there was no edema and only minimal signals of inflammation in paws of BFO-treated rats. Higher doses of BFO exhibited greater inhibitory effects than smaller doses, suggesting a dose-dependent action. Conclusions: These results suggest, for the first time, the potential of BFO as a source of molecules with antiinflammatory activity, justifying one of its main popular uses. Financial support: CNPq/Brazil.

\



93

CODE 86 INCLUSION COMPLEXES OF FUROSEMIDE WITH β-CYCLODEXTRIN

AND PVP Campos, M. L. G (1), Silva-Júnior, A. A.(1,2)*.

1Laboratório de Tecnologia e Biotecnologia Farmacêutica – TecBioFar – Departamento de Farmácia – UFRN. 2Programa de Pós-Graduação em Ciências Farmacêuticas da UFRN. Corresponding author:[email protected] Introduction: Furosemide is a diuretic drug used in the treatment of hypertension. However, this drug is practically insoluble in water, and strategies that may modify its physico-chemical properties as aqueous solubility it is very important for production of the oral solid dosage forms. Objective: The mainly aim of the present study was to investigate the conditions for obtaining inclusion complexes of β-FZ with the CD and hydrophilic polymers such as PVP in solution. Materials and Methods: The phase solubility study was performed to evaluate the complexation in solution at 30 + 2ºC using the Higushi and Connors model. The quantitative analysis of drug was performed by UV-VIS spectrophotometry at 277nm (n=5). The analytical methodology was validated according to analytical parameters established by ANVISA (Brazilian Sanitary Vigilance Agency). The association of a third component, like PVP, was also investigated. Results and Discussion: The solubility diagrams showed the formation of inclusion complexes of FZ with B-CD with stoichiometry of 1:1 M with a stability constant of 166M-1. An increment in the FZ solubility about 4 times was obtained. The association of B-CD (0.015 M) and the PVP was increased solubility about 6 times compared to water. Conclusions: The results indicate that the potential use of ternary complexes and the effect of PVP on the increment of the solubility of the Financial support: Brazilian Agency CNPq, PROPESQ-UFRN



94

CODE 87.1 RATIONAL USE OF DRUGS IN HYPERTENSION: AN ACHIEVEMENT

OF PHARMACEUTICAL CARE

Malheiro, J. P. T.1; Silva, P. C. D.1; Falcão, R. A.1; Cartaxo, N. A. de O.1*; Silva, S. A. S.1; Portela, A. S.2; Queiroz, M. S. R.3; Simões, M. O. 3

1 Discentes de Farmácia, Universidade Estadual da Paraíba (UEPB) 2Programa de Desenvolvimento e Inovação Tecnológica de Medicamentos, Universidade Federal do Rio Grande do Norte (UFRN) 3 Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB) Corresponding author:* [email protected]

Introduction: The Pharmaceutical Care is a professional practice model that uses the rational pharmacotherapy to achieve concrete results in response to prescribed therapy. It requires of the pharmacist, the translation of scientific knowledge and the promotion of rational use of drugs, the main therapeutic tool. The irresponsible use of drugs may mask symptoms or to propitiate drug interactions and adverse effects. Objective: Based on this context, the study aimed to evaluate the use of medications for hypertension assisted by a group of pharmaceutical care. Materials and Methods: It was a cross-sectional study, whose sample consisted of 100 hypertensive patients assisted by the Program for Pharmaceutical Care (PROATENFAR) of UEPB. The information in this analysis were obtained by means of a questionnaire developed and validated previously, applied by the same interviewer. The data were treated with the Statistical Package for the Social Scienses (SPSS) version 17.0. Results and Discussion: The drugs used by the sample covered 29 medicines at doses as monotherapy or combined, the being drugs: hydrochlorothiazide (44%), captopril (33%), amlodipine (20%), Aspirin (26%), Atenolol (14%) and Propatilnitrato (11%), the most frequent. In the absence of any of the medicines available by the public service, over 90% of the sample said they did not discontinue therapy, using loans or purchases. And, 39.65% said the pharmacist or pharmacy students of PROATENFAR as information providers on the rational use of medicines. Conclusions: Thus, these results reinforce the importance and effectiveness of pharmaceutical care in patient adherence and in the promotion of rational use of medicines. Financial support from: Programa de Incentivo à Pós-Graduação e Pesquisa (PROPESQ), UEPB




95

CODE 87.2 EXTRACTION OF FLAVONOIDS AND POLYPHENOLS IN LEAVES OF

Mentha piperita L. PROCESSED IN FIXED BED CONVECTIVE DRYER Cartaxo, N. A. de O.1 Oliveira, A. R. 1 Apolinário, A. C. 1 Fialho, G. J. D. 1

Feitosa,V.A.1 Pachú, C. O.1,

1Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB); INTRODUCTION: Mentha x piperita L. is a species cultivated in semi-arid and traditionally used popular medicine. S nce ’s used by he SUS, should to observe the optimization of the drying process for preservation of bioactive compounds. OBJECTIVE: Study the drying process leaves Mentha x piperita L. and its effects on the extraction of polyphenols and flavonoids. METHODS: The study was conducted at the Laboratório de Meios Porosos e Sistemas Particulados at UFCG, Campina Grande – PB, from September/2009 to May/2010. Once collected, the samples were identified by the Herbário Lauro Pires Xavier (Nº 41899). The leaves were dried in a fixed bed convection dryer at temperature of 40, 50 e 60ºC and then macerated using 70% ethanol solution. In the resulting extracts were carried out determinations of the compounds, for spectrophotometry, total polyphenols and total flavonoids, using the Folin-Denis – standard tannic acid (760nm) and aluminum chloride solution - standard quercetin (425nm), respectively. RESULTS: The drying proved to be fast, being completed at intervals of 125, 110 and 100 minutes on average to temperatures of 40, 50 and 60ºC, respectively. Quantification of total polyphenols in average was 18.71, 13.36 and 9.43 µg/mL for 40, 50 and 60ºC, respectively. For total flavonoids quantified the mean was 9.80, 7.01 and 5.44 µg/mL for 40, 50 and 60ºC, respectively. CONCLUSIONS: The leaves of Mentha x piperita L. had higher concentrations of the compounds quantified in the temperature of 40ºC in fixed bed convection dryer, proving the best methodology. Work funded by the Programa de Iniciação Científica / UEPB.



96

CODE 90 DISTRIBUTION OF TRIATOMINES FAUNA AND NATURAL INFECTION

BY Trypanosoma cruzi IN THE SEMIARID OF THE STATE OF RIO GRANDE DO NORTE, BRAZIL Silva, A. N. B.1; Martins, K.2; Câmara, A. C. J.1, 2; Chiari, E.3; Galvão, L. M. C.1, 4; 1. Programa de Pós-Graduação em Ciências Farmacêuticas, CCS, UFRN 2. Departamento de Análises Clínicas e Toxicológicas, CCS, UFRN 3. Departamento de Parasitologia, ICB, UFMG 4. Programa de Pós-Graduação em Ciências da Saúde, CCS, UFRN Corresponding author:[email protected] Introduction: The predominant triatomines species with potential for transmitting Trypanosoma cruzi and ability for adapting to various ecotopes in the semiarid are Triatoma brasiliensis and T. pseudomaculata. Objective: To evaluate the triatomines fauna and natural infection by T. cruzi in different semiarid environments. Materials and methods: The insects were captured manually in peridomicile, intradomicile and wild environment of different municipalities in west and central mesoregions. The insects were identified and examined by the direct method and xenoculture for detecting T. cruzi. Results and discussion: Of the 779 captured triatomines, 43.4% were T. brasiliensis (59.8% adults and 40.2% nymphs), followed by 27.6% of T. pseudomaculata (83.7% adults and 16.3% nymphs), 26.2% of Panstrongylus lutzi (only adults) and 2.8% of Rhodnius nasutus (63.6% adults and 36.4% nymphs). The species captured in intradomicile were T. brasiliensis (95.5%) and T. pseudomaculata (4.5%). In peridomicile were found T. brasiliensis (52.3%), T. pseudomaculata (35.4%) and R. nasutus (12.3%). In wild environment were captured 36.4% of T. brasiliensis, 36.6% of P. lutzi and 27% of T. pseudomaculata. The rate of natural infection of triatomines by T. cruzi was 9.8%. The highest infection rate, 29.9%, was detected in P. lutzi, while T. brasiliensis was 4.1%, T. pseudomaculata 3.2% and R. nasutus 0.0%. Conclusion: T. brasiliensis and T. pseudomaculata were captured in different stages and in all environments. Infected nymphs in the peridomicile and adults inside the houses show the adaptability to anthropic environment and their potential as vector, which suggests the need for continuous epidemiological surveillance actions. Financial support from CNPq/MCT/CNPq; FAPERN/PPSUS; DCR/CNPq/FAPERN



97

CODE 91 DEVELOPMENT OF THIOPHENES DERIVATIVES BIOCOMPATIBLES

MICROEMULSIONS Pedrosa, M.O.1; Araújo, G.M. F.1; Silva, N.E.S2.; Patriota, Y.B.G.2; Pereira, R.C.2, Silveira, W.L.L.3. Ferreira, L.F.3, Silva-Filho, M.A.3, Egito, E.S.T.3, Mendonça-Jr, F.J.B.4, Silva, J.A.1, Damasceno, B. P. G. L.1

1Universidade Estadual da Paraíba (UEPB), Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Laboratório de Desenvolvimento e Caracterização de Produtos Farmacêuticos (LDCPF), 58429-600, Campina Grande-PB-Brazil, 2UEPB, LDCPF, Departamento de Farmácia, 58429-600, Campina Grande-PB-Brazil. 3Universidade Federal do Rio Grande do Norte (UFRN), Departamento de Farmácia, Laboratório de Sistemas Dispersos (LASID), 59010-180, Natal-RN-Brazil. 4UEPB, PPGCF, Laboratório de Síntese e Vetorização de Moléculas (LSVM), João Pessoa-PB-Brazil. Introduction: Thiophenes derivatives (TD) are strong insoluble molecules and have important structural fragment of many pharmaceutical compounds (aromatic heterocyclic group). They exhibit significant action against fungals, bacterias, viruses, worms and insects. Several TD were syn hes zed by UEPB’s ese ch g oup, keLSVM-5CN06 and LSVM-6CN10. Objective: To develop and to characterize a LSVM-5CN06oil-in-water microemulsion(LSVM-5CN06-ME) and LSVM-6CN10 oil-in-water microemulsion (LSVM-6CN10-ME). Materials and Methods: The ME was prepared by a direct mixture of four inactive ingredients: Lipoid® S 100, Tween® 80, Mygliol® 812 N and distilled water.The TD were previously dissolved in chloroform and added under stiring into the ME. The solvent was evaporated by a rotative evaporator, and the macroscopic aspect, pH and conductivity were analyzed. Results and Discussion: The solubility test has shown that LSVM-5CN06 was insoluble in acetronitrile and methanol, and soluble in chloroform; the LSVM-6CN10 was sparingly insoluble in acetronitrile and methanol, and soluble in chloroform. The pH values were 5,68±0,12 and 7,59±0,016 to LSVM-5CN06-ME and LSVM-6CN10-ME, respectively.To the electrical conductivity, the values were97,83±23,39 and 625,9±7,41 to LSVM-5CN06-ME and LSVM-6CN10-ME. Conclusions: These results allow us to conclude that ME is a good delivery system to LSVM-5CN06 and LSVM-6CN10. However, other future aspects need to be investigated for a better analysis of the system. Financial support from PROPESQ/UEPB.



98

CODE 92 INFLUENCE OF PLURONIC® F68 ON THE BENZATHINE PENICILLIN G

ANTIBACTERIAL ACTIVITY Silva, K. S.¹; Silva, K. G. H¹; Silva, K. C. H¹; Alexandrino –Junior, F¹; Godoy, J. S. R²; Svidzinski, T. I. E²; Egito, E. S. T¹#. 1, Laboratório de Sistemas Dispersos, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN) 2 Laboratório de Micologia Clínica, Departamento de Farmácia, Universidade Estadual de Maringá (UEM) Corresponding author:[email protected]#

Introduction: Polymer-based nanotechnology became one of the most attractive and fast growing areas of pharmaceutical research. In aqueous solutions at concentrations above critical their micelle concentration (CMC) these polymers self-assemble into micelles. Objective: The purpose of this study was to evaluate the antibacterial activity of Benzathine Penicillin G (BenGP) into a micellar solution (MS) of Pluronic®F68. Materials and Methods: Firstly the MS of Pluronic®F68 was prepared in water at its CMC of 1.23x10-4M at 37°C. Antimicrobial activity of BenGP (500µg/mL) into these Micellar Solution (MSBenGP) was evaluated by the determination of minimum inhibitory concentration (MIC) using such as blank the Pluronic®F68 MS and as a standard a BenPG solution at the same concentration against S. pyogenes ATCC19615, S. mutans ATCC25175, S. faecalis ATCC51299(1), S. faecalis ATCC29212(2) and S. aureus ATCC25923, cultivated as described in the Clinical and Laboratory Standards Institute. Results and Discussion: The measurement of the MICs for S. pyogenes was reduced from 1.953 to 0.488µg/mL, S. mutans from 1.953 to 0.122µg/mL, S. faecalis(1) from 0.977 to 0.244µg/mL, S. faecalis(2) from 0.977 to 0.061µg/mL and S. aureus from 7.813 to 0.976 µg/mL using MSBenPG. The MS without of BenPG did not show antimicrobial activity. These results showed that MS enhances antibacterial activity of BenGP for all tested strains. Conclusions: There are, MS could be useful for treatment of infectious diseases against these microorganisms, leading to a significant reduction of drug used during a treatment, which also may decrease the number of injections and improves the patient compliance to the treatment. Financial Support from CNPq/Brazil and master scholarship from CAPES/Brazil (Procad NF 2008)




99

CODE 93 EVALUATION OF THE ANTIFUNGAL ACTIVITY OF NOR-LAPACHONE

DERIVATIVE AGAINST ISOLATES OF CANDIDA spp. Thé, M. F. D. F.1 ; Santos Neta, M. A.1,2 ; Silva, C. R.1,2, ; Andrade Neto, J

.B.1 ; Macedo, D. S.2,3; Silva Júnior, E. N.4; Pinto, M. C. F. R.4; Macêdo, D.

S.3; Cavalcanti, B. C.3; Magalhães, H. I. F.3 ; Moraes, M. O.3; Nobre Júnior,

H. V.1,2,3

1Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection and Experiments in Yeast (LABEL). Federal University of Ceará, Fortaleza, CE, Brazil. 2Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, CE, Brazil. 3Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil. 4Department of Chemistry , Federal University of Minas Gerais, Belo Horizonte, MG, Brazil Corresponding author: [email protected]

Introduction: Nor-lapachones, the inferior homologue of the β-lapachones, have been showed important biological properties. In a program for obtaining naphthoquinones more potent against Candida spp., we prepared a new compound (NPPN 3223) from 2-Allyl-3-hydroxy-[1,4]naphthoquinone. In recent investigation, NPPN 3223 was active against Trypanosoma cruzi ( he e o og c gen of he Ch g s’ d se se) and Lymphoma T Murine EL-4 cells. Objectives: Herein, the antifungal potential of NPPN 3223 against 04 strains of Candida spp was evaluated. Methods: A total of 04 strains of Candida spp. belonging to the Laboratory of Bioprospecting and Experimentation in yeast (LABEL) from Federal University of Ceará were used. The antifungal susceptibility testing was performed by broth microdilution method, according to the protocol of CLSI M27-A3. NPPN 3223 was tested at concentration ranging from 0.25 to 128 mg / mL. Strains of C.parapsilosis (ATCC 22019) and C.krusei (ATCC 6258) were used as control strains. Results and Discussion: The NPPN 3223 showed antifungal activity against all strains of Candida spp. Conclusion: The obtained results showed, under our experimental conditions, a cytotoxity action of NPPN 3223 on all Candida strains evaluated. However, studies with large numbers of strains are needed. Financial Support from FUNCAP, CNPq.



100

CODE 94 QUALITATIVE PHYTOCHEMICAL PROFILE OF Jatropha gossypiifolia

L. EXTRACTS PREPARED BY DIFFERENT METHODS OF EXTRACTION Gomes, J. A. S.1; Félix-Silva, J.1; Pinheiro, I. T. M. G.1; Zucolotto, S. M.2; Fernandes-Pedrosa, M. F.1*

1Laboratório de Tecnologia & Biotecnologia Farmacêutica (TecBioFar), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN) 2Laboratório de Farmacognosia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN) Corresponding author: [email protected] Introduction: Jatropha gossypiifolia L. (Euphorbiaceae), popularly known n B z s “p nhão- oxo”, s vege spec e used n fo k med c ne s n -inflammatory, antimicrobial, antiophidian, antipyretic, and hypotensive drug. Substances such as flavonoids, terpenoids and lignans have been isolated from different parts of the plant, however, the literature lacks systematic studies that differentiate the chemical composition of each plant organ, and the majority of these reports does not discern or specify which method of extraction was employed. Objective: Determine and compare the phytochemical profile of leaf, stems, and roots extracts from J. gossypiifolia prepared by different methods of extraction aiming future studies in screening of bioactive compounds. Materials and Methods: Leaves, stems and roots were dried at 60ºC and pulverized. Extracts of each part, separately, were prepared at 10% w/v (dry plant: solvent extractor) by infusion, decoction, ultrasound bath (using water as solvent extractor) and maceration (ethanol). These extracts obtained (twelve extracts) were compared qualitatively by phytochemical screening and thin layer chromatography. Results and Discussion: Alkaloids, flavonoids, gums, lactones, quinones, resins, sulfur compounds, tannins, and terpenoids were identified in J. gossypiifolia extracts. In general, leaf extracts presented better results (greater number of substances identified) than stems and roots, and aqueous extracts (even if obtained at different methods) showed similar profiles. Flavonoids were identified as the possible major substances of leaf extracts. Conclusions: This study highlights the potential of the specie as a source of bioactive molecules, opening ways for the study of this plant in order to obtain new therapeutic alternatives. Financial support: CNPq/Brazil.



101

CODE 95.1 EVALUATION OF ANTIFUNGAL ACTIVITY OF CRUDE EXTRACT

FROM Caesalpinia ferrea Mart AGAINST CLINICAL ISOLATES FROM THE ORAL CAVITY OF RENAL TRANSPLANTATION Ferreira, M. R. A.1; Santiago, R. R.1; Diniz, M. G.1; Milan, E. P.1; Faria, M. G. I.2; Mello, J. C. P.3, Svidzinski, T. I. E.2; Soares, L. A. L.1,4* 1Programa de Pós graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte (UFRN). 2Laboratório de Micologia Médica, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá (UEM). 3Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá (UEM). 4Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco(UFPE). Corresponding author:[email protected] Introduction. The increases in the drug resistance as well as the several side effects from current antifungal therapy reinforce the importance and the potential of plants extracts and their secondary metabolites as alternatives for new, more effective and less toxic treatments. Objective. In this context, the aim of this study was to evaluate crude extracts of C. ferrea against clinical isolates from the oral cavity of renal transplantation. Materials and Methods. The extractive solutions were obtained by turbo extraction (water or acetone:water, 7:3) and then were filtered, concentrated and lyophilized to yield the crudes extracts (CE). The CE were evaluated against 91 isolates (Candida spp.) obtained from Natal/RN and Maringá/PR. The minimum inhibitory concentration (MIC) was determined according to CLSI with adaptations for natural products. The data were analyzed by the MIC, MIC50 and MIC90. Results and Discussion. The both CE (aqueous and acetone:water) of C. ferrea showed the same MIC (ranged from 3.9 to 1000µg/ml), MIC50 and MIC90 (125 and 500µg/ml, respectively), against C. albicans. Concerning the activity against C. non-albicans the CE extracts showed similar performance, and the MIC was ranged from 3.9 to 500µg/ml, while MIC50 and MIC90 were 31.25 and 500µg/ml, respectively. Conclusion. To conclude, the significant antifungal activity observed for both CE extracts, suggest that the plant species can play an important role as a potential antifungal agent. However, complementary studies are necessary to obtain fractions and isolated compounds. Financial support: CAPES and CNPq.



102

CODE 95.2 BIOASSAY-GUIDED PURIFICATION OF CRUDE EXTRACT FROM

STEM BARKS FROM Caesalpinia ferrea Mart (PAU FERRO) Ferreira, M. R. A.1; Mello, J. C. P.2; Svidzinski, T. I. E.3; Soares, L. A. L.1,4*

1Programa de Pós graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte (UFRN). 2Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá (UEM). 3Laboratório de Micologia Médica, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá (UEM). 4Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco(UFPE). Corresponding author:[email protected] Introduction. Caesalpinia ferrea (Caesalpiniaceae) is a tree native from Brazil, known as pau ferro. Because of its widespread use, research is needed to identify which are the active compounds of the species. Objective. The aim of this study was to investigate an antifungal activity by the bioassay-guided of a crude extract (CE) from C. ferrea. Materials and Methods. The steam barks of C. ferrea were submitted to turbo extraction with acetone:water (7:3), yielding the CE. The CE was partitioned with ethyl-acetate/water (1:1), resulting in aqueous (FW) and ethyl-acetate (FEtOAc) fractions. The FEtOAc was subjected to column chromatography (Sephadex®LH-20) with an eluent gradient EtOH (50%, 100%), MeOH (50%, 100%) and acetone:water (7:3), to yield 12 subfractions (F1-F12). The fractionation was monitored by thin layer chromatography (TLC) and the antifungal activity was evaluated by the CLSI using strains ATCC of Candida spp. Results and Discussion. The CE was partitioned yielding the FA and FEtOAc. The evaluation of the antifungal activity showed that the FEtOAc was more active, with MIC between 0.97–31.25µg/ml. Thus, the FEtOAc was selected for new purification process and 12 subfractions were obtained. The subfractions were monitored by TLC and antifungal activity, and the fraction from F8-F11 presented most intense spots on TLC, typical for polyphenolic compounds of high molecular weight, and even better antifungal activity, which MICs were ranged from 1.95-250µg/ml. Conclusion. The bioassay-guided purification of crude extract from C. ferrea suggested that the antifungal activity seem to be attributed to the polyphenolic composition of the drug material. Financial support: CAPES and CNPq.



103

CODE 96 IDENTIFICATION AND PHOSPHOLIPASE ACTIVITY OF FUNGI

ISOLATED FROM DERMATOMYCOSES OF CHILDREN ATTENDING MUNICIPAL CENTERS OF CHILDREN EDUCATION FROM NATAL-RN Silva, W.P1; Lemos, V.L.B1; Chaves, G.M1

1Laboratório de Micologia Médica e Molecular – Departamento de Análises Clínicas e Toxicológicas – Universidade Federal do Rio Grande do Norte (UFRN) Corresponding author:[email protected] Introduction: Dermatomycoses are superficial mycoses which damage the cutaneous tissue. They are extremely prevalent among agglomerated environment, where people share personal objects. Objective: The aims of the present study were to isolate and investigate phospholipase among fungi obtained from superficial infections from children who attend Municipal Centers of Children Education, at Natal-RN. Materials and Methods: Children from 0 to 6 years old were evaluated. They belonged to the foundations: Amor de Mãe, at Praia do Meio; Maria Eulália, at Passo da Pátria community and Professora Maria Itacira Bento, from Cidade da Esperança neighborhood. Clinical samples were collected from children showing lesions probably due to dermatomycoses. Samples were smeared on the surface of Sabouraud Dextrose Agar with Cloramphenicol (100 mg/L), and incubated at room temperature (26 + 1 °C), for up to 15 days. Yeasts identification was performed with the chromogenic medium CHROMagar Candida®, microculture slides and classic methods. Phospholipase activity was determined by growing yeast cells on the surface of phospholipase agar. Results and Discussion: We collected samples from 102 patients, and obtained 55 positive results (54%). Positive results are distributed as follows: 22 yeasts from the genus Trichosporon (40%), 10 yeasts from the genus Malassezia (18.2%), 10 C. parapsilosis (18.2%) 7 C. albicans (12.7%), 3 P. ohmeri (5.45%), 1 C. tropicalis (1.82%), 1 Cryptococcus sp. (1.82%) and 1 isolate of T. rubrum (1.82%). Conclusions: C. albicans isolates had high phospholipase activity. C. parapsilosis and P. ohmeri strains showed intermediate levels of phospholpase activity. C. tropicalis, Cryptococcus sp. and Trichosporon spp. Isolates showed low or none phospholipase activity. .



104

CODE 97 EVALUATION OF THE REPRODUCTIVE AND FETAL TOXICITY OF

CYLINDROSPERMOPSIN IN THE RAT Almeida, C.S.1; Arruda, A.C.C.1; Queiroz, E.C.1; Costa, H.T.M.L.1; Barbosa, P.F.1; Lemos, T.M.A.M.2; Oliveira, C.N.3; Schwarz, A.1; Pinto Ernani 4; Kujbida, P.1

1Laboratório de Toxicologia, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN). 2Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN). 3Departamento de Patologia, Universidade Federal do Rio Grande do Norte (UFRN). 4Laboratório de Toxinas e Produtos Naturais de Algas, Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo (USP). Corresponding author: [email protected] Introduction: Cylindrospermopsin (CYN) is a naturally occurring alkaloid produced by different genera of freshwater cyanobacteria, e.g. Cylindrospermopsis, Umezakia, Aphanizomenon, Raphidiopsis. The CYN toxicity is characterized by fatty degeneration of the liver, cholestasis, injury to renal tubules and thymic atrophy. Objective: In the present study, the potential of CYN to induce toxic effects and to impair gestation in rats exposed orally during pregnancy was investigated. Materials and Methods: The animals were exposed to 0.03, 0.30, 3.00 µg CYN/kg of body weight/day, for 21 days, by oral administration (gavage). These doses were lower than the no-observed-adverse effect level (NOAEL) used to establish the guideline for a safe level of drinking water for this toxin (1 µg/L) by World Health Organization (WHO). Females were sacrificed on day 21 of gestation, when maternal body and organ weights were recorded, serum and biochemical analyses were performed, reproductive parameters (number of pregnant rats, body and reproductive organ weight, number of implantation sites, viable fetuses, and resorption sites) were determined and skeleton and viscera of fetuses were evaluated. Results and Discussion: The reproductive performance and teratology results were similar between control and exposed group, however, cyanotoxin administration increased the size of the kidneys and the urea rates in pregnant rats. Conclusions: Our findings suggest that the consumption of CYN in the concentration recommended by WHO is not able to promote fetotoxicity or affect the reproductive performance of rats. On the other hand, orally administered CYN may induce initial nephrotoxicity in pregnant mammals. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil



105

CODE 98.1 DEVELOPMENT OF A LIQUID DETERGENT FORMULATION FOR USE

IN UFCG/CES AND EVALUATION OF VISCOSITY AND POWER FOAM Azevedo, M. G. B; Almeida, M. A. A. L. S.; Batista, M. T.; Souza, J. B. P.; Alencar, J. S. Farmácia Escola Manoel Casado de Almeida, Curso de Bacharelado em Farmácia, Universidade Federal de Campina Grande/Centro de Educação e S úde, O ho D’águ d B c , s/n, Cu é, PB, 58175-000. Corresponding author: [email protected] Introduction: The cleaning materials are sanitizing consumed in large scale, including in universities, in which the bidding processes for acquisition, takes time-consuming and often are products of low quality and yield. Among these articles, the liquid detergent is one of the most consumed products by UFCG/CES.Objective: Aiming this need, the objective of this study is to develop a formulation of liquid detergent to be used in UFCG/CES, evaluating viscosity and foaming power. Materials and Methods:Thus, twelve formulations were prepared asa pilot formulation.The first eight contain surfactants, stabilizers, hidrótopos, preservatives and additives, with the concentration of the agent variable viscosity. The remaining four used identical components to those used in the previous group, varying only the concentration of primary surfactant Sodium laureth-2 sulfate (LESS).Results and Discussion: The manipulation of the first formulations allowed the construction of the Critical Micelle Curve (CMC) and showed that regardless of the concentration of the thickener was impossible to obtain the ideal viscosity for samples of commercial reference. In the subsequent four, set the viscosity with increasing concentration of less, thus obtaining the optimal formulation consisting of sulfonic acid (5%), Sodium hidroxide (1%), Triethanolamine (0.4%), LESS (5%), amphotericbetaine (1%), Dietanolamida (0.5%), Viscopon (1.5%), urea (1%), isothiazolinone (0.25%), core (qs), essence (0,1%) and NaCl (0.8%), with medium viscosity of 413 cP and foam height of 5.5 cm, compatible with the benchmark. Conclusions:The success of this formulation meets the need of that educational institution, providing a product with high-quality in relation to the bid, seeking a better cost-effective.



106

CODE 98.2 RETROSPECTIVE STUDY OF ADVERSE DRUG REACTIONS IN THE

PEDIATRIC WARD OF THE UNIVERSITY HOSPITAL ALCIDES CARNEIRO, CAMPINA GRANDE / PB Almeida, M. A. A. L. S.1; Azevedo, M. G. B.1; Oliveira, H. F. A.1; Jácome, R. C.1; Neto, A. R. M.2; Lima, D. D. F. S.1; Farias, A. D.1; Porto, M. L. S.3 1 Universidade Federal de Campina Grande/Centro de Educação e Saúde, O ho D’águ d B c , s/n, Cu é, P íb , B s Em : 2 Acad. Enfermagem, Universidade Estadual da Paraíba, Campina Grande, Paraíba 3MSc Farmacêutica-Bioquímica - gerente de risco do Hospital Universitário Alcides Carneiro, Campina Grande, Paraíba Corresponding author: [email protected] Introduction: The limitation in pre-clinical and clinical studies on the use of medicines in children, often leads the profissional to interpret the prescribing drug therapy in a pediatric equivalent of what is practiced in adults. This fact compromises the result and safety of treatments, and can trigger Adverse Drug Reactions (ADRs). Objective: To describe the main ADRs n ped c Un ve s y Hosp ‘A c des C ne o’ membe of he Sentinel Network of National Health Surveillance Agency (ANVISA). Material and Methods: Retrospective and descriptive study, based on reporting forms developed by ANVISA and recorded through an active search on Sentinel Hospital during the period 2002 to 2010. Study was carried out with the aid of didactic literature and the electronics teaching one. Results and discussion: During that time, 52 notifications were made, these were 31.0% in pediatrics. In that sector it was noted that the ADRs hives were more frequent (46.6%), pruritus (20.0%), fever (13.3%), vomiting, edema, headache, tremors pyrogenic shock, and covered 6.6% of ADRs. The most common drugs responsible for that damage was the sodium dipyrone and ceftriaxone in 53.3% of total pediatrics ADRs. Children can vary pharmacokinetic and/or pharmacodynamic, that may lead to an increase or decrease in sensitivity to some drugs, whose causes are not always known. Conclusion: The study shows that such changes require the use of medications in children should be further studied and monitored by the pharmacist, during therapy.



107

CODE 99 IN VITRO RELEASE OF METHOTREXATE-LOADEDSPRAY DRIED

PLGA MICROPARTICLES Oliveira, A. R.1,2, Abrantes, J. A.1,2, Oliveira, A. G.3, Silva-Junior, A. A.1,2. 1Pharmaceutical Technology and Biotechnology Laboratory, Federal University of Rio Grande Norte (UFRN), Natal – Brazil; 2 Graduate Program in Pharmaceutical Sciences (UFRN), Natal – Brazil; 3 Graduate Program in Pharmaceutical Sciences (UNESP) – Araraquara – SP – Brazil. Corresponding author:[email protected] Introduction: Methotrexate is a chemotherapeutic that has a rapid plasmatic elimination. Biodegradable microparticles have been extensively investigated to prolong the drug release. The study of drug release profile is essential to ensure success and safety of these drug delivery systems. Objective: To assess the in vitro release profiles of different PLGA microparticles containing MTX obtained by spray drying.Materials and Methods: MTX-loaded PLGA microparticles were obtained in a mini spray dryer Buchi-191 at different ratios drug/polymer (10, 20, 30 and 50% w/w). The in vitro release studies were performed in phosphate buffer pH 7,4 (0.05M KH2PO4) medium thermostatized 37°C ± 0,2°C. At established intervals, the tubes were centrifuged and then the supernatant were analyzed by UV-Vis spectrophotometry. Cumulative drug released were fitted using different kinetic release models: zero order, fist order, Higuchi and Weibull treatments. Results and Discussion: The in vitro release studies performed for all microparticles systems shown a MTX sustained release. The time necessary to release all drug content was 1, 2, 8 hours for PLGA microparticles containing 50, 30 and 20% w/w MTX respectively. However, the time necessary for release about of 80% of drug content in the microparticles containing 10%w/w/ of drug was 1008 hours. The analysis of drug release revealed a prevalent Weibull model, which was evident by the correlation coefficient (r2 = 0.985).Conclusions: PLGA microparticles containing methotrexate were successfully obtained by spray drying technique, which demonstrating an extended release profile for all drug-polymer ratios used.

Financial support: Brazilian Agency CNPq, CAPES




108

CODE 101 BENZNIDAZOLE-LOADED SOYBEAN O/W EMULSION SYSTEM

Streck, L.1*; Araújo, M. M.1; Santos, S. R. A.2 ; Souza, I2; Oliveira, A G3; Silva-Júnior, A.A.1

1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte, UFRN, Natal, RN 2Curso de Farmácia, UFRN, Natal, RN 3Faculdade de Ciências Farmacêuticas de Araraquara, UNESP, Araraquara, SP Corresponding author:[email protected] Introduction: Benznidazole (BNZ) is not only the drug of choice for

Chag s’ d se se e men , bu now d ys s so he on y one d ug avaible in Brazil. However, BNZ is poorly soluble drug and it is available just as tablets. Objective: Developing a soybean o/w emulsion system (ES) containing BNZ. Materials and Methods: Soybean oil was use as oil phase and a mixture of span® 80 (Sigma Aldrich, EUA) and tween® 80 (Synth, Brazil) as surfactants, deionized water as aqueous phase and BNZ (Roche, Brazil). An excess of BNZ was loaded using the ready emulsions at room temperature or added on inversion phase moment at 70ºC. The effect of different co-surfactants such as ethyl alcohol, benzyl alcohol and methyl-1 pyrrolidone-2 and the proportion of the components were investigated, which was estimated using UV-Vis epectrophotometry, validated previously, and varying proportions of the components from ES were studied when modified the ratios of soybean oil and surfactant mixture. Results and Discussion: BNZ loaded in the O/W ES was about 0.04 and 0.039% for technique at room temperature and and 70°C, respectively. The different methods and co-surfactants used did not provoke statistical differences on drug loaded. Increasing the rate of soybean oil declined the BNZ loaded however, the rate surfactants studied did not induce changes in the BNZ loaded. Conclusions: The experimental results showed that the different methods and surfactants used did not change statistically the drug loading. The oil phase increment leads to decreasing of these levels. Financial support: CNPQ and CAPES



109

CODE 102 EVALUATION OF CITRONELLA (Cymbopogon winterianus JOWITT

EX BOR.) OIL COMPOSITION EXTRACTED FRESH AND DRY PLANT AND INFLUENCE OF HARVESTING PERIOD. Silva, M. E.1; Carbogim, L. G. S.1; Silva, T. M.2; Amaral, M. P. H.1; Cheider,

L. M.3

1Pharmacotechniques Laboratory, Pharmaceutical Department, Juiz de Fora Federal University (UFJF); 2Food and Toxicology Laboratory, Food and Toxicology Department , Juiz de Fora Federal University (UFJF). 3Phytochemistry Laboratory, Botany Department, ICB, Juiz de Fora Federal University (UFJF). Corresponding author:[email protected] Introduction: The essential oil extracted from leaves of citronella grass (Cymbopogon winterianus Jowitt ex Bor.) has aromatic and therapeutic features, and raw material for the industry in repellents and toilet preparations. Among the many factors that can influence the chemical composition of volatile constituents stand out from the time of harvest and drying process. Objective: Evaluation of the influence of harvest time and drying the composition of oil, compared with the fresh plant. Materials and Methods: Plant Origin: Medicinal Plants Garden, Pharmacy Faculty, UFJF. Harvesting periods: August 2010, January and April 2011. For this study, extractions were performed by hydrodistillation in Clevenger apparatus, of fresh leaves (FL) and dried leaves (DL) between 32-35ºC for 7 days. The essential oil samples were analyzed by GC/MS. Results and Discussion: During August 2010, it was observed in the essential oil of FL the presence of α-muroleno, γ-cadinene and farnesol and in April 2011, in the essential oil of DL, the presence of cis-2,6-dimethyl-2,6-octadieno-acetate and ge n o , h w sn’ found n o he pe ods. The g e e qu n es of found components in all samples were citronellal, geraniol and citronellol. Regarding the drying process, there was a reduction in the number of substances identified from the DL, when compared with FL. Conclusion: The different times of harvest influenced the number of components found in essential oils. The drying method affected the essential oils causing a reduction in the number of components detected in samples taken from the dried leaves. Financial support from: Pharmaceutical Department, Pharmacotechniques Laboratory - UFJF



110

CODE 103 SCREENING FOR ANTIBACTERIAL ACTIVITY ANTIMICROBIAL

ACTIVITY OF HYDROALCOHOLIC EXTRACT OF Anadenanthera macrocarpa Benth AND Myracodruom urundeuva All. AGAINST STRAIN OF Staphylococcus aureus Almeida, C. L. F.1; Nunes, L. E.1; Fernandes, A. F. C.1; Viana, A. P. P.1; Oliveira, A. S. C.1; Catão, R. M. R.2 ¹Pharmaceutical Sciences Graduate Program of State University of Paraiba (UEPB) 2Center of Biological and Health Sciences, Pharmacy Department, State University of Paraiba (UEPB) Corresponding author:[email protected] Introduction: Anadenanthera macrocarpa (AM) Benth - Fabaceae, known s “ ng co” e ex ens ve y used n fo k medicine for the treatment of coughs, whooping cough, bronchitis, inflammation, healing and antioxidant. Myracrodruon urundeuva (MU) All.-Anacardiaceae, popu y “ oe -do-se ão”, d ug use h s nc e sed due o he p esence of nn ns, used against upper respiratory tract, urinary tract, hemoptysis, and metrorrhagia. Objective: The aim of this study was to evaluate the in vitro antimicrobial activity of hydroalcoholic extract of AM and MU, compared to strains of Staphylococcus aureus outpatient source. Materials and Methods: Was used as test microorganisms the standard strain S. aureus ATCC 25923, and strains of S. aureus home care (UEPB). The study was performed by disk diffusion method, 20μL was added to extract discs with sterile filter paper 6 mm in diameter, previously identified. The plates were incubated at 37ºC/24h and read thereafter. Results and Discussion: The inhibition halos formed from the activity of the extract of AM and MU varied between 8 and 14mm. The results show that the use of medicinal plants such as antibacterial therapy, may be quite feasible for the discovery of substances with biological activity. Conclusion: All examined extracts showed antibacterial activity against the strains of S. aureus tested. Financial support: CAPES.


http://pt.wikipedia.org/wiki/Fabaceae



111

CODE 104 OUABAIN REDUCES THE PHAGOCYTOSIS OF PERITONEAL

MACROPHAGES Leite, J. A1; Alves, A.K.A. 1; JACOB, P. L.2; GALVÃO, J.G.F.N.1; 2Néris, P. L. N.; 2Oliveira, M. R.; Rodrigues-Mascarenhas, Sandra1

1Centro de Biotecnologia, Laboratório de Imunologia, Universidade Federal da Paraíba (UFPB) 2Departamento de Biologia Molecular e Celular, Laboratório de Leishmaniose, Universidade Federal da Paraíba (UFPB). Corresponding author:[email protected] Introduction: Ouabain, a potent Na+, K+-ATPase inhibitor, was identified as an endogenous substance of human plasma. Our group have demonstrated the ability of ouabain to modulate inflammatory processes, but little is known about the mechanisms involved. Phagocytosis is a complex process, important for immune responses against pathogens and resolution of inflammation. Objective: The aim of this work was to investigate, in vitro, the modulating effect of ouabain on phagocytosis. Materials and Methods: The cytotoxic effect of ouabain on peritoneal macrophages was determined by MTT. Macrophages (2x105 cells/well) were cultured in the presence of different ouabain concentrations (10nM, 100nM and 1000nM) for 24 hours. For the phagocytosis assay, cells were also exposed to zymosan A (5x106 per well). Cytokine concentration was assayed using an ELISA kit (Bio Science). Results and discussion: Ouabain showed no cytotoxicity in all concentrations studied. However, ouabain 100nM suppressed phagocytosis (20%), but did not affect phagocytosis index, as well as the levels of pro- and anti-inflammatory cytokines analyzed. Conclusions: Ouabain interferes with the phagocytic function of macrophages. This effect is independent of IL-1, IL-6, TNFα and IL-10 release. However, further studies are necessary to elucidate the mechanisms involved. Financial Support: The study was conducted with financial support from CNPq / Brazil.



112

CODE 105 EVALUATION OF THE RELAXING ACTIVITY OF ETHANOL EXTRATCT

FROM LEAVES ASPIDOSPERMA MACROCARPUM IN ISOLATED RAT MESENTERIC ARTERY.

Oliveira M. P.1; Paulino, J. V. S.1; Aquino, P. G. V.2; Sant'Ana, A. E. G.2;

Ribeiro, Ê. A. N.1; Araújo-Júnior, J. X.2

1Escola de Enfermagem e Farmácia, UFAL, Maceió/AL 2Instituto de Química e Biotecnologia, UFAL, Maceió/AL, Brazil. INTRODUCTION: Aspidosperma macrocarpum Mart. is a plant popularly known s “pe oba-gigante-do-ce do”. Seve k o ds h ve been isolated from the genus Aspidosperma. These alkaloids have several pharmacological properties, such as: α-adrenergic blocking activity, hypotensive and analgesic (J. Pharm. Biom. Ana. 12:10, 1994; J. Pharm. Sci. 62:11, 1973). In a previous work, our group has demonstrated hypotensive activity of Aspidosperma macrocarpum in non-anesthetized Wistar rats (Oliveira et al, 2011).OBJECTIVE: The aim of this study was to investigate the relaxing activity of ethanolic extract Aspedosperma macrocarpum (EEAM) in superior mesenteric artery isolated from rats Wistar.MATERIALS AND METHODS: The plant was collected at Planaltina (GO) in November 2007, and identified by Professor José E. de Paula. The voucher specimen is deposited in the herbarium of University of Brasilia (UnB), encoded as JEP 3767. The leaves extract was obtained by maceration in 90% ethanol by 48 hours, and the EEAM was obtained after removal of the solvent. From 1.6 Kg of the dry vegetal material were obtained 517 g of EEAM. Rat superior mesenteric rings (2-4 mm) were suspended by co on h e d fo some c ens on eco d ngs n Ty ode’s solution at 37ºC and gassed with a 95% O2 and 5% CO2, under a resting tension of 0.750 g. Statistical analyses were pe fo med by s uden “ ” es . The study was approved by the ethics committee of the Federal University of Alagoas (010151/2008-82). RESULTS AND DISCUSSION: In isolated rat superior mesenteric rings, increasing concentrations of EEAM (0.1, 0.3,

1, 3, 10, 30, 100 and 300 g.ml-1) were able to antagonize the effects of

phenylephrine (10 M) and KCl (80 mM) induced contractions induced

contractions (EC50= 273,30,84 g.ml-1 and 323,30,5 g.ml-1, respectively). However, the smooth muscle relaxant activity of EEAM was

not atenuated by endothelium removal (EC50= 116,60,48 g.ml-1). CONCLUSIONS: These results demonstrated that the non-specific vasorelaxante activity induced by EEAM can not be attributed to the participation of vascular endothelium. Further experiments are necessary to elucidate the mechanisms involved.

Financial support: FAPEAL, CAPES and CNPq.



113

CODE 106 EVALUATION OF TESTS PRE-TRANSFUSIONAL IMMUNO-

HEMATOLOGICAL IN RECEPTORS OF BLOOD Batista, M.T1; Ferino, L.P.P.2; Leal, K.D.B. 1,2,3 Academic Unit of Health, Education and Health Center, Federal University of Campina Grande (UFCG). Corresponding author: [email protected]

INTRODUCTION: The hemotherapy in Brazil, has been characterized by the development and adoption of new technologies with the objective at minimizing the risks of transfusion, especially with regard to preventing the spread of infectious and contagious agents (Carrazzone, 2004). With regard to blood receptors, the Ministry of Health (Decree 1376/93) determines the tests immune-hematological such as pre-transfusional ABO / Rh classification, research irregular antibody (RIA) and compatibility testing (crossmatch). OBJECTIVES: This study aimed to describe the importance of immune-hematological testing pre-transfusional performed in blood banks. MATERIAL AND METHODS: This study was compiled from a literature review, with research in books, scientifics magazines and scientific article. The method chosen to perform the blood type and Rh factor was the use of "Cards", the DiaMed-ID®, which is contained in a "card" the blood type, Rh factor and reverse tests, and other "Card" RIA's research and direct Coombs test. RESULTS AND DISCUSSION: The points discussed in this review show that the technique using "cards" for carrying out the immune-hematological testing has proven effective and fast with a high level of security (Ferreira, 2010), as it was not reported in no work the presence of immune-hematological reactions after transfusion, showing no incompatibility for the receivers. CONCLUSION: The points discussed in this study show that qualitative measure of immune-hematological tests pre-transfusion taken to a safe blood transfusion have been improved, making it more safe for the realization of the realization of hemotherapy.



114

CODE 107 DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE LIQUID

CHROMATOGRAPHY METHOD FOR THE EVALUATION OF RECOMBINANT HUMAN INTERLEUKIN-11. CORRELATION WITH THE BIOASSAY

Souto R. B. 1, Calegari G. Z. 1, Walter M. E. 1, Cardoso Junior C. D. A. 1, Stamm F. P. 2, Dalmora S. L. 1

1Department of Industrial Pharmacy, 2 Postgraduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, 97105-900 Santa Maria-RS, Brazil Corresponding author: [email protected] (Dalmora S. L.)

Introduction: Interleukin 11 is a multifunctional cytokine of the IL-6 type subfamily of long-chain helical cytokines, which modulates the proliferation, differentiation and maturation of various types of hematopoietic cells. Recombinant human interleukin-11 produced by DNA technology is currently being used worldwide for the prevention of thrombocytopenia and to reduce the need for platelet transfusions after myelosuppressive chemotherapy in patients with nonmyeloid malignancies. Objective: The aim of this study was to develop and validate a stability-indicating reversed-phase liquid chromatography (RP-LC) method for the content/potency assessment of rhIL-11 and correlate the results to the bioassay.Materials and Methods: The LC method was carried out on a Jupiter C4 column (250 mm x 4.6 mm i.d.), maintained at 25°C. The mobile phase A consisted of 0.1% trifluoroacetic acid (TFA) in water and mobile phase B consisted of 0.1% TFA in acetonitrile. The gradient elution was performed at a flow rate of 1 mL/min with UV detection at 214 nm. The biological potency was evaluated using the bioassay based on the proliferative activity of the TF-1 cells.Results and Discussion: The RP-LC method validation demonstrated acceptable results for the linearity, accuracy, precision, sensitivity, specificity and robustness, without any interference from the excipients. Biopharmaceutical batches of rhIL-11 were analysed by the RP-LC and by the bioassay, giving potencies between 93.23 and 103.83% of the stated potency, with significant

correlation (p0.05). Conclusion: The RP-LC method represents an alternative which can be used in combination with the bioassay, contributing to improve the quality control and to assure the therapeutic efficacy of the biotechnology-derived product. Financial support: CNPq




115

CODE 108 STUDY OF A RP-LC METHOD FOR THE ANALYSIS OF

RECOMBINANT PARATHYROID HORMONE IN BIOPHARMACEUTICAL FORMULATIONS

Stamm, F. P b, Butzge, C. S a, Chiossi, K. a, Calegari, G. Z a, Souto, R. B a, Dalmora, S. L. a

Department of Industrial Pharmacy a, Postgraduate Program in Pharmaceutical Sciences b, Federal University of Santa Maria, 97105-900 Santa Maria-RS, Brazil Corresponding author:[email protected] (Dalmora SL)

Introduction: Human parathyroid hormone, hPTH, is a single-chain polypeptide of 84 amino acids secreted by the cells of the parathyroid glands. It is a major regulator of blood calcium levels. Recombinant human parathyroid hormone produced by DNA technology in Escherichia coli, consists of a 34 amino acids with a molecular mass of 41 kDa. It is currently being used for the treatment of postmenopausal osteoporosis in women as well as primary or hypogonadal osteoporosis in men. Objective: The aim of this study was to develop a reversed-phase liquid chromatography (RP-LC) method for the evaluation of rhPTH (1-34) in biotechnology-derived products. Materials and Methods: The LC method was carried out on a Zorbax 300SB C18 column (150 mm x 4.6 mm i.d.), maintained at 45°C. The mobile phase consisted of 0.2 M sodium sulfate buffer pH 2.3 and acetonitrile (65:35, V/V). The gradient elution was performed at a flow rate of 1 mL/min with UV detection at 214 nm. Results and Discussion: The chromatographic separation was obtained with the retention time of 12 min and was linear over the concentration range of 10 - 300 µg/mL (r2=0.9995).The specificity was tested showing that there was no interference of the excipients of the formulation in the analysis. The method was applied for the analysis of rhPTH (1-34) giving potencies between 98.72% and 104.39%. Conclusions: The method was partially validated and applied for the analysis of rhPTH (1-34) in biopharmaceutical formulations, contributing to the research of alternative which improve the quality control assuring the therapeutic efficacy of the biological medicine.

Financial support: CNPq



116

CODE 109 EFFECT OF TRIETHANOLAMINE/METHYL-1-PYRROLIDONE-

2/BETACYCLODEXTRIN MULTICOMPONENT COMPLEXES SYSTEMS ON THE BENZNIDAZOLE APPARENT SOLUBILITY Melo, P. N.1; Rocha, H. C. S.2; Silva-Junior, A. A.1

1Graduate Program on Pharmaceutical Sciences, Federal University of Rio Grande do Norte (UFRN), Natal –RN, Brazil. 2Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, UFRN, Natal –RN, Brazil. Corresponding author:[email protected] Introduction: Benznidazole is a poor soluble drug and the only available for Chagas disease treatment in Brazil. Tthe increment on the its aqueous solubility is an interesting alternative. Objectives: The purpose of this study was to check the influence of betacyclodex n (β-CD), triethanolamine (TEA) and methyl-1-pyrrolidone-2 (MP), and to establish the cosolvency or complexant mechanism of both TEA and MP in change of BNZ aqueous solubility. Materials and methods: The solubility studies were performed using the phase solubility diagrams described by Higushi nd Conno s (1965). The nf uence of he ssoc on of β-CD (0-0,015 mol.l-1) with TEA (10%) and MP (10%) was also investigated. The cosolvency or complexing mechanism was elucidated using the Jain and Yalkowsky method (2007), in which the the cosolvency occurs when a semi log plot Sw (solubility in water)/Smix (solubility in the co-solvent solution) versus fraction of solubilizer (f) (TEA or MP) is linear. Results and discussion: The increment on the BZN solubility was about 1.72; 4.9 and 28 fold for β-CD, TEA, and MP, respectively. The association of TEA or MP with β-CD lead to increase of 1.56 (β-CD 0-0,015 mol.l-1 + TEA 10%) and 3 times (β-CD 0-0,015 mol.l-1 + MP 10%). It was identified a consolvency mechanism for TEA, while MP presented also a complexant effect. Conclusion : TEA and MP increase the BNZ solubility significantly. However, the association with TEA and MP did not show a synergic effect. Financial support from CNPq/Brazil CNPq



117

CODE 111.1 PHYSICAL PARAMETERS OF QUALITY ASSESSMENT OF

DIFFERENT LOTS OF HOMEOPATHIC GLOBULES IN USE IN NATAL /RN/BRAZIL Cabé, C. M.; Queiroz, A.; Navarro, M. V. M.; Aragão, C. F. S. Quality Control of Drugs Laboratory – LCQMed – Pharmacy Department – Federal University of Rio Grande do Norte - Brazil Introduction: Homeopathic globules are obtained industrially from sucrose or mixture of sucrose and lactose. The composition of them may be affected by factors such as temperature and humidity. Moreover, the method used for impregnation can have an effect on factors such as dose uniformity and weight of the globules. Objective: This study aims to evaluate the quality of globules used in the preparation of homeopathic products, used in Natal/RN/Brazil. Material and methods: Homeopathic globules, inert and impregnated with EtOH70%, in three different lots were assessed by determining the physical parameters. The friability test was carried out by friabilator and rotated 100 times (25 rpm for 4 min). The hardness test was carried out by using two different hardness tester (manual and automatic tester). The weight uniformity test was carried out as follows: 20, 50 e 100 globules from each brand were selected, dusted and weighed individually.Adequate globules hardness and resistance to powdering and friability are of paramount importance for consumer. Recent studies have explained relationship that exists between globules hardness and its disintegration and more significantly drug dissolution. Results and discussion: Regarding theweight, no significant changes were observed in inertlots, compared to impregnated withEtOH70%. No significant changes were observed for friability tests in inert and impregnated globules. The hardness test showed a decrease in values observed in impregnated as compared toinert globules, demonstrating a possible reduction of the hardness due to the presence of the vehicle.



118

CODE 111.2 SPECTRAL ANALYSIS (FT-IR, UV) OF RELEASE OF LEACHABLES

FROM NATURAL RUBBER BULBS Cabé, C. M.; Navarro, M. V. M.; Aragão, C. F. S. Quality Control of Drugs Laboratory – LCQMed – Pharmacy Department – Federal University of Rio Grande do Norte - Brazil Introduction: Chemical bonds vibrate at specific frequencies, which are determined by the mass of the constituent atoms, the shape of the molecule, the stiffness of the bonds, and the periods of the associated vibrational coupling. Objective: In this study, we realized spectral analysis (FT-IR, UV) of release of leachables from natural rubber bulbs by factorial design. Material and methods: Natural rubber bulbs were analyzed with infrared and UV spectrometry. Rubber bulbs from West® samples were prepared with the help of factorial design.90mgof the bulbs were extracted with 10ml of different solvents (chloroform, n-hexane, acetone and ethanol-30, 70 and 100%). Results and discussion: The extractive solutions showed characteristics transitions of possible transfer of materials: natural

rubber was characterized by the vibrations of2920 (m) and 2852(F), (CH),

3437(F), (OH), 1617 (m) (C=C) and 1384 (F), (C=O). The bands corresponding to the presence of the accelerator were found in 3557 (F)

and 3406 (F) (NH). The bands found in 3412 (F) (S=O) corresponds to the vulcanizing agent. Conclusions: According to data obtained by UV spectrometry, the samples followed the order of release of constituents of the rubber in the following order: CHCl3>acetone> n-hex>EtOH>70% EtOH>30% EtOH.



119

CODE 112 TOXICITY OF DENTAL VARNISH CHITOSAN

Cavalcante, H. M. M.1,3; Lidiane Pinto Correia1; Thayza Christina Montenegro Stamford1; Sergio Luiz Dalmora2; Rui Oliveira Macêdo1

1Department of Pharmaceutical Sciences, Unified Development Laboratories and Essays of Drugs, Federal University of Paraíba, 2Department of Industrial Pharmacy, Federal University of Santa Maria, 3

Nucleus of Health Research of Integrated Colleges of Patos- Brazil Corresponding author:[email protected] Introduction:Chitosan, a cationic amino polysacch de, essen y β-1,4 D-glucosamine (GlcNAc) linked to N-acetyl-D-glucosamine residues, is naturally present in the cell wall of certain fungi, and can also be obtained by chitin deacetylation. Objective: The objective of this study was to evaluate the toxicity of dental varnish chitosan in chorioalllantoic membrane, NCTCclone929 and CHO cell culture. Material and methods:The toxicity of the dental varnish chitosan was evaluated inchorioallantoic membrane of chick embryo at 10th day of incubation. The eggs were obtained from GuaravesGuarabira Aves Ltda and incubated at 37ºC. Thecytotoxicitystudieswere conductedbyneutral reduptake assayusingNCTCclone929 and CHO cell culture. Results and discussion:The dental varnish chitosan evaluated inchorioallantoic membrane promotedvasoconstriction, coagulation and bleedingwhich was

initiated at the following time (seconds) 133 7.35, 205 4.97 and 169 5.72,respectively.The DL50 values obtained for dental varnish chitosan in NCTCclone929 and CHO cell culture were: 0.2064 mg ml-1 and 0.3691, respectively.Conclusions:The dental varnish chitosan was shown to be irritating inchorioallantoic membrane. The results of cytotocity corroborate with the of chorioallantoic membrane. . Financial support from CNPq/Brazil.



120

CODE 113 NEW TALENT PROGRAM – EDUCATION FOR HEALTH – ANALYSIS

AND EDUCATION IN WATER USE IN THE OF TRAIPU – ALAGOAS. Silva Júnior, E. F.; Reys, J. R. M.; Trindade1, R. F. C; Nascimento1, T. G.; Silva, M. E. B2.; Silva, A. T.; Souza, R. S1.; Mbambi, S. J. T1.; Amado, A. P.2; Silva, I. M.1; Silva, P. H. V. M1. 1Escola de Enfermagem e Farmácia, Coordenador do Curso de Farmácia, Universidade Federal de Alagoas – UFAL 2Faculdade de Medicina, Universidade Federal de Alagoas – UFAL 3Centro de Tecnologia, Engenharia-Química, Universidade Federal de Alagoas – UFAL Corresponding author: [email protected] Introduction: With actual greats problems involve the Water in several parts of the world, become each time more essential to work the question of the conscience of people of one shape general. That work was realized in Traipú – AL, where it appears on second place on the raking of cities with worse IDH of country. Materials and Methods: The work was accomplished with 30 children and 30 teachers, both of public and municipal schools. Were used banners for exposition of pictures related w h he w e ’s w s e, po u on, d se ses nd o he s; compu e s fo presentation of documents pptx; books for to help on building of educational dramatiz on bou good p c ces fo vo d he w e ’s waste. Results and Discussion: Was clear the city have biggest needs w h e on he ques ons of po cs of w eness fo vo d he w e ’s waste and pollution. There was, after the realize works, had a major advance on question educative referent the water. Conclusions: The work was an awesome way of awareness for the target-public, leading a level of understands major about the discussions involving in Water. But, know there more for to make, because is a continuous work and can expand for other regions. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil.



121

CODE 114 EVALUATION OF THE CARDIOVASULAR RISK IN CONNECTION TO

C-REACTIVE PROTEIN Ramos, K. R. L. P.¹; Silva, J. R. S.2; Muniz, T. T. M.2; Sousa, A. P. B.2; Ramos, R. A.2; Rocha, W. R. V.2; Peixoto, J. B. S.3; Guimarães, N. E. S.4; Clementino, F. R. B.4; Cunha, M. A. L.5. ¹Pharmaceutical Sciences Graduate Program of State University of Paraiba (UEPB) 2 Pharmaceutical Degree of State University of Paraiba (UEPB) 3 Public health Graduate Program of State University of Paraiba (UEPB) 4 Lab technician of State University of Paraiba (UEPB) 5 Center of Biological and Health Sciences, Pharmacy Department, State University of Paraiba (UEPB) Corresponding author [email protected] Introduction: The C-Reactive Protein (CRP) is an acute phase protein, synthesized by the liver in answer to cytokines, that reflect active systemic inflammation constituting an inflammatory marker considered as a strong predictor independent of risk for a cardiovascular event. Objective: The analysis of the us-CRP in patients assisted in a Basic Unit of Family Health. Methodology: The study was of the transversal type, documental, descriptive and analytical whose data was analysed statistically through the Epi-Info version 3.4. Between august of 2010 and august of 2011. The biochemical determinations were made in the Metrolab 2300 and the us-CRP was dosed by chemiluminescence in automated equipment IMMULITE 1000. Results and Discussion: The sample was constituted of 104 patients, being 18.3% of the male genre and 81.7% female, of which 48.1% correspond to the age group of 60-80 years. It was observed that 35.5% of the patients were hypertensive and had type 2 diabetes mellitus, of these, 25% were classified in accordance to us-CRP as high risk (p< 0.009). After analysis it was observed that 42% presented elevated cholesterol with 24% of high risk for us-CRP. As for the HDL-cholesterol, 82.7% of the patients showed altered values, being 47.1% with high risk for us-CRP. Conclusion: A great relevance was observed of the research of us-CRP as a way of identifying the subclinical inflammation what will contribute to the preventive strategies aiming the reduction of risk factors that contribute to the development of Cardiovascular Diseases. Financial support from CNPq/Brazil.



122

CODE 115 CHEMICAL PROFILE VERIFICATION OF THE EXTRACTS AND SEMI-

PURIFIED FRACTIONS of Andira nitida Mart. ex Benth BY TLC Ferreira, A. V. D¹, Moraes, M. A.²; Fonseca, B. A. D.²; Guia, F. C.², Monteiro, D. K.²; Moreira, G. A.³, Santana, M. C.³; Machado, A. K. M. S.³; Silva, A. E. G.³; Simas, N. K.³, Zanetti, G. D.³, Konno, T. U.³; Leite, M. N.²; Rocha, F. D.2,3

1Mestrando em Ciências Farmacêuticas, UFJF, 2 Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade Federal de Juiz de Fora (UFJF). 3Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ-Macaé).

Corresponding author [email protected]. Introduction: Andira nitida (Fabaceae) is part of the plants popularly known as Angelins, which are species rich in isoflavones, flavanols, rotenoids. Objective: Evaluating the chemical profile of the A. nitida branches Hex / DCM 8:2 and EtOH: H2O 8:2 extracts, as well as semi-purified fractions. Material and Methods: The botanical material was collected at restinga of Jurubatiba in Quissamã-RJ, Brazil. After drying and grinding, 1.2 kg of material was extracted consecutively with Hex / DCM 8:2 and EtOH: H2O 8:2. The lyophilized obtained from hydroalcoholic extract was purified with solvents of increasing polarity (hexane, CHCl3, EtOAc, BuOH) on ultrasound. For CCD profiles verification, 10mg/mL solutions were prepared from dry crude extracts and semi-purified fractions. Solutions (5mg/mL) of eugenol, coumarin apigenin, standard steroid, quercetin, sparteine, terpene standard, rutin, tannic acid, coumaric acid and catechin were used as standards of comparison. 5μL were applied on silica gel plates. After development with several eluent systems, the dry plates were visualized under UV light (254 and 366nm), then they were reveled with ceric sulfate, anisaldehyde sulfuric acid, vanillin sulfuric acid, 10% FeCl3 in MeOH, Dragendorff, NP-PEG, KOH 1N, 0.2% DPPH in MeOH. Results and Discussion: Compounds as terpenes, steroids and phenolic derivatives (especially flavonoids and tannins) were identified. In addition, some substances with antioxidant potential in the hydroalcoholic extract and their fractions could be visualized. Conclusion: The conditions for analysis by TLC showed adequated for identification of secondary metabolites classes present in organic extracts and their fractions. These results are consistent with the literature. Financial support from FAPERJ, CNPq, PROPG



123

CODE 116 PRO-INFLAMMATORY AND CYTOTOXIC ACTION CARRAGEENAN OF

THE SEAWEED AMANSIA MULTIFIDA Souza, L. A. R.*; Cruz, A. K. M. *; Leite, E. L. * 1 Laboratory of vegetal Glicobiology, Department of Biochemistry, Federal University of Rio Grande North (UFRN) Introduction. Carrageenan are sulfated polysaccharides present in cell walls of red algae and well known in the food industry as gelling and to induce inflammation process in animal model. Objective. This work aims to evaluate the pro-inflammatory and cytotoxic effects of polysaccharide fraction extracted from red algae Amansia multifida. Materials and Methods. The pro-inflammatory effects in vitro were determined by the ability of fractions cause hemolysis by the alternative pathway of complement system (CS) and direct cell hemolysis (10, 25 and 50 µg of sample) and in vivo by testing acute peritonitis in swiss mice (n =5), determining the amount of leukocytes and the interleukin-6 (IL-6) concentration of the peritoneal cavity (dose of 25 and 50 mg/kg of body weight (BW)). The in vitro cytotoxicity was performed using mouse fibroblast cells (3T3), evaluated by MTT method, with incubation time of 24 and 48 h (10, 50 and 100 µg of sample). Results and Discussion The fraction had no directly lysis effect on red blood cell tested in the masses, however, caused stimulation of up to 42.6% (50 µg) (p<0.001) of hemolysis by the alternative pathway of the CS. The fraction promoted an increasing of 313.8% (25 mg/kg BW) and 670.1% (50 mg/kg BW) in cell migration when compared to group treated with saline (p<0.001). The dosage of IL-6 showed values of 45.8 pg/mL (25 mg/kg BW) and 120.2 pg/mL (50 mg/kg BW). Conclusions Wasn´t observed a significant cytotoxic effect of 3T3 cells treated with the fraction in 24 h of incubation. However, with 48h observed cell death of 79% (100 µg) (p<0.001). The results in question on the use of carbohydrates as additives in foods, cosmetics and medicines. Financial supported by CNPq/Brazil



124

CODE 117.1 ANTIFUNGAL ACTIVITY OF MEDICINAL PLANTS AGAINST CANDIDA

SPECIES Guimarães, G. P.1; Santos, R. L.2; Medeiros, A. C. D.2; Vieira, K. V. M.3. 1Universidade Estadual da Paraíba (UEPB), Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Laboratório de Desenvolvimento e Caracterização de Produtos Farmacêuticos (LDCPF), 58429-600, Campina Grande-PB-Brazil. 2UEPB, PPGCF, Laboratório de Desenvolvimento e Ensaios de Medicamentos (LABDEM), Campina Grande-PB-Brazil. 3UEPB, Departamento de Farmácia, Laboratório de Microbiologia, Campina Grande-PB-Brazil.

Corresponding author [email protected]

Introduction: The use of medicinal plants has been widely seen as basis for health maintenance. It is commonly found in female disorders such as oral and vulvovaginal candidiasis caused by Candida species. The increasing resistance to usual antifungal agents demands for research on new sources, such as medicinal plants. Objectives: To evaluate the antifungal activity of medicinal plant against the main Candida species related to oral and vulvovaginal candidiasis. Materials and Methods: Was evaluated the antifungal activity of hydroalcoholic extract (70%) made from med c n p n s used n B z n fo k med c ne: “ f v c -de-c boc o” (Hyptis Mutablis B q.), “ cum” (Annona coriacea L.), “ me x ” (Ximenia americana L.), “b ún ” (Schinopsis brasiliensis Eng .) nd “c p cho-de-c g no” (Acanthospermum hispidum DC.). The test was performed against the species Candida albicans (ATCC 18804), C. guilliermondii (ATCC 6260), C. krusei (ATCC 34135), C. parapsilosis (ATCC 22019) and C. tropicalis (ATCC 13803) using the agar diffusion method with cylinders. The results were expressed as the average diameter of the growth inhibition zone around the cylinders. The experiments were performed in triplicate. Results and Discussion: The extract of braúna (Schinopsis brasiliensis Engl.) was active against the tested species, and minimum inhibitory concentration (MIC) at a dilution of 25 and 50% to C.guilliermondii and C. parapsilosis respectively. The extracts from other plants has not shown activity against the yeasts. Conclusions: The leaf extract of braúna can be further studied and the extract or her (s) active (s) possibly can be used to obtaining alternative drugs against vulvovaginal and oral candidiasis caused by these Candida species. Financial support from CNPq-PIBIC/UEPB.




125

CODE 117.2 DEVELOPMENT OF A LSVM-5CN05 TRANSDERMIC

MICROEMULSION Guimarães, G.P.1; Mendonça-Jr, F.J.B.1,2, Damasceno, B. P. G. L.1, Silva, J.A.1

1Universidade Estadual da Paraíba (UEPB), Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Laboratório de Desenvolvimento e Caracterização de Produtos Farmacêuticos (LDCPF), 58429-600, Campina Grande-PB-Brazil. 2UEPB, PPGCF, Laboratório de Síntese e Vetorização de Moléculas (LSVM), João Pessoa-PB-Brazil. Corresponding author [email protected] Introduction:Thiophenes derivatives (TD) are strongly insoluble molecules with several biological activities, especially against fungal species of genus C nd d nd Aspe g us. Seve TD we e syn hes zed by UEPB’s research group, like 2-[(3,4-dichloro-benzylidene)-amino]-5.6-dihydro-4H-ciclopen-ta[b]thiophene-3-carbonitrile (LSVM-5CN05). Microemulsions (ME) are defined as systems thermodynamically stable and optically transparent formed from a mixture of two immiscible liquids stabilized by an interfacial film of surfactants. These systems can be aimed to develop therapeutic agents based on drugs hardly incorporated due to their high lipophilicity, such as LSVM-5CN05. Objective: To develop and to characterize a LSVM-5CN05-ME. Material and Methods: The microemulsion was prepared by a mixture of Labrasol® (S), Plurol Oleique® (CS), isopropyl myristate (O) and distilled water (W). The LSVM-5CN05-ME was obtained by adding the drug to the mix of components S, CS and O by stirring in Ultra-Turrax T25 at 9000 rpm for 3 minutes. Then, the distilled water was added to this mixture, followed by stirring and ultra-sound bath and the macroscopic aspect, refractive index and pH were analyzed after 48 hours. Results and Discussion: The preparation of the LSVM-5CN05-ME at 400µg mL-1 result in a system clear, isotropic and translucent, with the refractive index values of 1.410 (blank ME) and 1.412 (LSVM-5CN05-ME). The pH values were 4.10±0.04 and 4.18±0.02 to blank ME and LSVM-5CN05-ME, respectively. Conclusions: This ME system appears to be a good delivery system to theLSVM-5CN05 and other aspects will be investigated for better analysis of the system. Financial support from PROPESQ/UEPB.




126

CODE 118 ACUTE AND SUBACUTE TOXICITY OF Caesalpinia ferrea STEM

BARK EXTRACT Vasconcelos, C.F.B.1; Maranhão, H.M.L.1; Menezes, M.H.G.2; Limeira, M.M.F.2; Lima, C.R.1; Dimech, G.S.2; Gonçalves, E.S.1; Costa-Silva, J.H.3, Wanderley, A.G.1,2 . 1Department of Pharmaceutical Sciences, 2Department of Physiology and Pharmacology, 3Department of Physical Education and Sport Sciences, UFPE, Brazil. Introduction: Caesalpinia ferrea Martius (Leguminosae) has been used for the treatment of diabetes, however there are no toxicological studies about its safety use. Objectives: To evaluate acute and subacute toxicity of aqueous extract of stem bark of C. ferrea on hematological parameters of Wistar rats. Materials and Methods: In acute test, the extract was administered at dose of 2000 mg/kg for both sexes (n=5/group/sex) and changes in body weight, behavior, warning signs and mortality were observed for 14 days. In subacute test, animals were treated orally with vehicle (control group, C) or aqueous extract of stem bark of C. ferrea in doses of 300 (T1) or 1500 (T2) mg/kg/day for 30 days. At the end of the treatment, animals were anesthetized with Nembutal (35 mg/kg, ip) and blood samples were collected for hematological analysis. Results and Discussion: Acute dose of C. ferrea 2000 mg/kg produced no signs of toxicity or death in rats. In subacute test, no deaths or toxic signs were observed. In addition, there were no changes in hematological parameters: Eritrocytes (C=8.43±0.13, T1=8.52±0.19 and T2=8.42±0.20 106/mm3), hemoglobin(C=16.07±0.34,T1=16.29±0.43 and T2=15.88±0.39 g/dL), hematocrit (C=47.20±0.92, T1=47.47±1.12 and T2=46.11±1.04%), platelet count (C=750.40±28.71, T1=716.90±31.29 and T2=657.80±34.07 103/mm3), leucocytes (C=16.01±0.99, T1=11.46±0.78 and T2=15.41±1.16 10³/mm³), neutrophils (C=10.43±0.85,T1=10.47±0.83 and T2=11.15± 0.81%), eosinophils (C=0.89±0.31, T1=0.26±0.22 and T2=0.19±0.18 %), basophils (C=0.56±0.05,T1=0.44±0.08 and T2=0.35±0.04%), lynfocytes (C=84.85±1.13, T1=85.17±1.04 and T2=83.32±1.44 %) and monocytes (C=3,27±0.39, T1=3.66±0.35 and T2=4.99±0.87 %). Conclusions: The acute and subacute oral administration of aqueous extract of stem bark of C. ferrea presented no toxicity in Wistar rats.



127

CODE 119.1 ANTINOCICEPTIVE ACTIVITY OF CURINE ON THE ACETIC ACID

ABDOMINAL CONSTRICTION TEST (WRITHING TEST) IN MICE Salgado, P. R. R.1; Leite, F. C.1; Fonsêca, D. V.1; Piuvezam, M. R.1; Almeida, R. N.1

1Laboratório de Psicofarmacologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB) Introduction: Curine is the major structural bisbenzylisoquinoline alkaloids that is isolated from the root barks of Chondrondendron platyphyllum, a medicinal plant from the northwest region of Brazil belonging to the Menispermaceae family. It has been reported on literature that some species from this family exert activity on central nervous system (CNS). Objective: This study aims to investigate the possible antinociceptive effects of curine through the acetic acid abdominal constriction test (writhing test) in mice. Material and Methods: Swiss male mice were used, 25-35 grams, 3 months old (N=8). The negative control group received distilled water and tween 80; the experimental group was treated with curine (2.5 mg/kg) and positive control, morphine (10 mg/kg; i.p.). In the writhing test, the animals were treated with acetic acid 1.0%, and then observed the latency to the onset of the first contortion and the total number of contortions displayed by each animal. Results and Discussion: Treatment with curine (2.5 mg/kg) induced a significant increase in the latency to the onset of the first contortion compared to the control group (267.2±10.9 vs 217.7±10.8) and induced a significant reduction in the number of contortions (35.4±2.2) compared to the control group (47.8±1.3). These results show that curine (2.5 mg/kg) has, at least preliminarily, antinociceptive activity, demonstrated by the writhing test. Conclusions: These results suggest that the curine has antinociceptive activity. Therefore, more studies are needed to determine whether the antinociceptive activity of the type reported is central, peripheral or involving the two pathways. Financial support: CNPq.



128

CODE 119.2 EVALUATION OF THE ANXIOLYTIC EFFECT OF ETHANOLIC

EXTRACT OF Sidastrum micranthum (MALVACEAE) ON THE ELEVATED PLUS-MAZE TEST IN MICE. Salgado, P. R. R.1; Carvalho, F. L.1; Torres, P. A.1; Pereira, W. B.1; Gomes, R. A.1; Almeida, R. N.1; Morais, L.C.S.L.1

1Laboratório de Psicofarmacologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB) Introduction: It is estimated that about 25% of all modern medicines are directly or indirectly derived from plants. The object of this paper is the extract of Sidastrum micrathum, a species of the family Malvaceae, unprecedented with regard to pharmacological studies. Objective: Evaluate whether Sidastrum micranthum produces a pharmacological action similar to anxiolytic drugs using the elevated plus-maze method in mice. Matherials and Methods: Swiss male mice were used, 25-35 grams, 3 months old (N=8). The negative control group received distilled water and Tween 80, the experimental group were treated with ethanolic extract of the Sidastrum micranthum (250 mg/kg) and positive control, diazepam (0.5 mg/kg). All administrations were performed intraperitoneally. After treatments, the animals were submitted to the elevated plus maze apparatus for 5 minutes. Results and Discussion: The ethanolic extract of Sidastrum micranthum (250 mg/kg) showed no significant changes in compared to control group in the different parameters observed, such as: number of visits to the open and closed arms (3.1±0.9 vs 2.3±0.9; 6.6±0.9 vs 5.5±1.1, respectively) and time spent in open and closed arms (20.9±5.9 vs 18.5±7.6; 199.8±26.0 vs. 246.1±17.9, respectively). The results show that the extract in question, despite having CNS depressant effects demonstrated in previous studies, has not anxiolytic drug profile. Conclusions: These data suggest that the ethanolic extract of Sidastrum micranthum not change the exploratory ability of animals in the elevated plus-maze testing, might not have anxiolytic activity, which is still a previous report, requiring further studies to validate it. Financial support: FAPESQ.



129

CODE 122 SCANNING ELECTRON MICROSCOPY (SEM) / ENERGY-

DISPERSIVE X-RAY SPECTROSCOPY (EDS) AS AN METHOD FOR THE CHARACTERIZATION OF MICROPARTICLES. Lima Neto, S. A. de2, Batista, C. C. N.2, Vargas, M. R. W. de3, Torres, S. M.4, Souza, F. S. de2, Oliveira, E. de J.1

1Laboratório de Tecnologia Farmacêutica, Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB).

2Laboratórios Unificados de Desenvolvimento e Ensaios de Medicamentos, Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB).

3Laboratório de Desenvolvimento de Medicamentos. Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN).

4Departamento de Tecnologia Mecânica, Centro de Tecnologia, Universidade Federal da Paraíba (UFPB).

Corresponding author: [email protected] Introduction. Solid dispersions (SD) are of great current interest as a formulation strategy. This is because if can be used to increase the effective solubility and bioavailability of insoluble active pharmaceutical ingredients. This work was aimed to characterize SD of glibenclamide (GB) with Polyethylene glycol (PEG1500/6000) and Colloidal silicon dioxide (AER) obtained by spray drier (SDy). Objective. Characterization of microparticles obtained by spray drier by SEM / EDS. Materials and Methods. Solid dispersion (GB+AER+PEG1500/6000) were analyzed by SEM recorded in a LEO1430, using a voltage of 10 or 20 kV (backscattered electron (BSE) image), after they have been gold sputtered. EDS spectroscopy image (dot-mapping) analysis of SD, was done by compressing the SD into a cellulose matrix (120 mg cellulose : 80 mg SD) and pressed at 8,0 tons. The resulting tablet was polished with sandpaper of three different grades (down to 1 micron), after what it was gold sputtered and analyzed. Results and Discussion. A complete and homogeneous dispersion of the active pharmaceutical ingredient (GB), represented by the elements chlorine, nitrogen, sulfur and silicon in the tablet was observed by EDS-dot-mapping. The distribution of characteristic elements of the GB is evidently seen as homogenous. The same cannot be observed with respect to GB obtained by others techniques. Conclusions. SEM / EDS was found to be a valuable tool in the evaluation and characterization of microparticles. The resolution of images obtained by EDS, provide a detailed observation of the morphology of the inclusions, compositional qualitative in this type of sample preparation. Financial support from CAPES/CNPQ/Brazil.




130

CODE 123 CLINICAL MODELS AND EVALUATION MEASURES IN THE STUDY OF

EXPERIMENTAL ANXIETY Chaves neto, G1; Almeida, RN 3; 4; Aragão, RF1; Braga, JEF 2; Trajano, FMP1. 1Graduating in Nursing from the Federal University of Paraíba. 2Department of Public Health Nursing and Psychiatry of the Federal University of Paraíba. 3Departament of Physiology and Pathology of the Federal University of Paraíba. 4Lab of Pharmaceutical Technology of the Federal University of Paraíba. Corresponding author: [email protected] Introduction. Anxiety is a universal experience characterized as a human emotional state like love, hate and blame. It is composed by emotional/behavioral and physiological factors. The discovery of drugs with therapeutic potential for anxiety in its pathological manifestation involves the development of scientific researches that use animal and human models of anxiety. In order to evaluate elicited anxiety in the human models for anxiety induction, it is commonly used psychological and/or physiological measures. The psychological measures implicate the use of psychometric scales and the physiological ones the measurement of parameters that reflect the organic changes produced or resulting from the elicited state of anxiety. Objective.The following review aims to present accumulated evidence in the literature around the main human models for induction of anxiety and the measures frequently used for its evaluation. Materials and Methods It was made a bibliographic research having as source of research articles listed by Pubmed and Medline databases and research in specific books which have investigated the main human models for experimental anxiety induction. Both the psychological and chemical models for anxiety induction present ethical limits and are constituted of fundamental links between basic research and therapeutic tests done with the patients. The psychological and/or physiological measures for evaluating experimentally produced anxiety reflect body changes that express the level of the elicited anxiety. Conclusions.The use of clinical models and evaluation measures in the study of experimental anxiety makes possible, under the scientific research, understanding the neurobiological substrate of anxiety in its pathological manifestation, beyond evaluating drugs with anxyolitic potential.




131

CODE 125 EVALUATION OF THE TYPE ACTION ANTIDEPRESSANT OF THE

EXTRACT HYDROALCOHOLIC OF Eupatorium ayapana Vent.

MELO, A.S¹.; CARVALHO, S2.; SILVA, J. B2.;BAETAS3, A. C.;SOUZA, P.J.C3; MAIA, C.S.F3

1Student of the Post-Graduate Pharmaceutical Sciences, College of Pharmacy, University Federal of Pará (UFPA). [email protected] 2Student of the Post-Graduate Pharmaceutical Sciences, College of Pharmacy, University Federal of Pará (UFPA). 3Professor of program in Pharmaceutical Sciences, College of Pharmacy, University Federal of Pará (UFPA). Introduction: The Eupatorium ayapana Vent., popularly known as japana is used in infusions, decoctions, like sedative, to combat insomnia, headache, etc.. Is widely used by the Amazonian population. Objective: The objective of this study was to evaluate the behavioral effects in the SNC, the hydroalcoholic extract (EHAEA) of the leaves and stems of Eupatorium ayapana Vent. Materials and Methods: This study evaluated the action of different doses of EHAEA on the behavior of Wistar rats, aged 2 months. We used eight groups of rats (n = 7) were divided into control (saline 0.9% ml/kg), anxiolytic action of standard drug (diazepam 1 mg/kg), standard drug action antidepressant (fluoxetine 10 mg / Kg) and 5 doses of the extract (100, 200, 400, 600, 800 mg / kg) . The administration was by oral. The behavioral test was the open field, the elevated plus-maze and forced swimming. To perform the experiment, animals were keept 1 hour before testing at the Laboratory of Experimental Pharmacology / UFPA, where testing was performed to acclimatization of the animals. Results and Discussion: The results in the open field test showed decreased locomotor activity of animals when compared with the control group. In the elevated plus-maze, the extract did not show anxiolytic activity in the forced swim and there was a reduction in immobility time compared to control, characterizing a type of antidepressant action of the extract. Conclusions: With these results, this paper aims to contribute to future studies,that can strengthen the use of extract of Japana in folk medicine. Financial support from Fapespa /Brazil



132

CODE 126 EXTRACTION, MEASUREMENT AND ANALYSIS OF TOTAL PROTEIN

OF NATIVE PLANTS FROM RN Menezes, Y. A. S.1; Pinheiro, I. T. M. G.1; Silva-Júnior, A. A.1; Uchoa, A. F.2; Pedrosa, M. F. F.1* 1 Laboratório de Tecnologia e Biotecnologia Farmacêutica, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN) 2 Laboratório de Química de Proteínas, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: In recent years the discovery of bioactive peptides increased exponentially. Noting the great potential that the plant kingdom presents as a source for new drugs, the extraction of compounds in plants has been an important source of identification of molecules with pharmaceutical properties. Objective: This study aims to isolate and characterize plant proteins with antimicrobial, anti-venom and anti-inflammatory activities. Materials and Methods: Samples of plant tissue Acmella uliginosa, Chenopodium ambrosioides, Euphorbia tirucalli, Jatropha molissima and Cnidoscolus urens L. were fragmented and macerated in liquid nitrogen. The extraction was performed in Tris-HCl 0.05 M NaCl 0.15 M, pH 7.5, at ratio of 1:2 (w / v) for 2 hours at 4 ° C while stirring. The extracts were evaluated by UV spectrophotometry at 280nm, measured by the BCA method, and evaluated by SDS-PAGE denaturing gel to 12%. The crude extract was fractionated by precipitation with acetone at 0.5, 1.0 and 2.0 volumes. Results and discussion: In the evaluation of protein content by spectrophotometry and measurement by BCA, we found that samples of leaves of C. urens (UV/280nm: 29605.44μg/mL and BCA: 15369.7μg/mL) and J. molissima (UV/280nm: 18775.51μg/mL and BCA: 9384.52μg/mL) stood out in the two methods applied. When analyzed by SDS-PAGE, the profile has changed, where the samples C. ambrosioides (sheet: 15 bands; root: 14 bands) and C. urens (sheet: 6 bands) stood out. Conclusion: Samples of C. ambrosioides and C. urens reported excellent results for the presence of proteins. The next stage of this work is testing of biological activity. Financial Support: CNPq/Brazil.



133

CODE 128 REPEAT-DOSE TOXICITY OF THE AQUEOUS EXTRACT OF

Caesalpinia ferrea STEM BARK Vasconcelos, C.F.B.1; Maranhão, H.M.L.1; Limeira, M.M.F.2; Menezes, M.H.G.2; Lima, C.R.1; Dimech, G.S.2; Gonçalves, E.S.1; Costa-Silva, J.H.3, Wanderley, A.G.1,2 1 Department of Pharmaceutical Sciences, 2 Department of Physiology and Pharmacology,

3 Department of Physical Education and Sport Sciences, UFPE, Brazil. Introduction: Caesalpinia ferrea Martius(Leguminosae) has been used in Brazilian traditional medicine, especially to treat diabetes. Objective: To evaluate the subacute toxicity (30days) of the aqueous extract of the stem bark of C. ferrea on biochemical parameters of rats. Materials and Methods: Male Wistar rats (280–300g) were divided into three groups (n=10/group). Animals were orally treated with vehicle (control group, C) and the aqueous extract of stem bark of C. ferrea at doses of 300(T1) and 1500(T2) mg/kg/day for 30 days. At the end of the treatment, animals were anesthetized with Nembutal® (35 mg/kg,i.p.), and blood samples were obtained for biochemical studies. Results and Discussion: No toxicity signs or deaths were recorded during 30 days of oral treatment with C. ferrea 300 and 1500 mg/kg. In biochemical analysis, it was observed an

increase (*p0.05) in amylase levels (C=498.20±33.22; T1=607.40±22.36*; T2=664.30±39.55* U/L), but no differences were observed in glucose (C=92.89±6.12; T1=98.78±3.52; T2=92.90±2.04 mg/dL); blood urea nitrogen (C=33.44±1.16; T1=29.56±1.65; T2=31.40±1.45 mg/dL); creatinine (C=0.71±0.03; T1=0.70±0.02; T2=0.65±0.02 mg/dL); aspartate aminotransferase (C=108.20±5.93; T1=106.20±6.67; T2=108.00±6.13 U/L); alanina aminotransferase (C=50.22±1.86; T1=51.89±1.70; T2=52.10±1.49 U/L); total bilirubin (C=0.100±0.005; T1=0.110±0.002; T2=0.120±0.006* mg/dL); direct bilirubin (C=0.07±0.01; T1=0.06±0.01; T2=0.10±0.01* mg/dL); indirect bilirubin (C=0.040±0.007; T1=0.050±0.006; T2=0.030±0.004 mg/dL); alkaline phosphatase (C=138.90±12.72; T1=130.70±5.16; T2=121.80±6.77 U/L); gamma-glutamyl transpeptidase (C=6.00±0.37; T1=5.56±0.18; T2=6.00±0.21 U/L); total cholesterol (C=99.56±11.13; T1=83.00±5.91; T2=78.20±3.35 mg/dL); uric acid (C=1.03±0.06; T1=0.96±0.06; T2=1.03±0.05 mg/dL). Conclusions: Oral treatment with aqueous extract of stem bark of C. ferrea for 30 days presented low toxicity in rats. However, the increase in serum amylase levels may be investigated in details.



134

CODE 129 PRELIMINARY STUDIES FOR THE USE OF ARTIFICIAL NEURAL

NETWORKS (ANN) IN THE STUDY OF RHEOLOGIC PROPERTIES OF TUBERCULOSTATICS AND SOLID EXCIPIENTS Navarro, M. V. M.1; Moura, T. F. A. L.1; Nóbrega, G. A. S.2; Lavor, E. P.3; Soares, D. S.3

1Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos, Universidade Federal do Rio Grande do Norte (PPgDITM/UFRN). 2 Programa de Pós-graduação em Engenharia Elétrica e Computação, Universidade Federal do Rio Grande do Norte (PPgEEC/UFRN). 3 Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte (PPgDITM/UFRN). Corresponding author: [email protected] Introduction: Artificial neural networks (ANN) are mathematical constructions able to learn the relationships between data. The ability of the interpretation and decision in complex systems make ANN as promising tools to optimize pharmaceutical formulations. Studies for the development of a solid formulation containing isoniazid, rifampsin, pyrazinamide and ethambutol showed that these drugs have serious flow problems. Objectives: This work aims at the development and use of optimizing ANN properties for optimization of flow properties of a powder mixture of tuberculostatic drugs. Materials and Methods: Backpropagation ANNs with a single hidden layer and two hidden layers were developed using Levenberg-Marquardt algorithm to adjust the synaptic weights. Carr index (IC), Hausner factor (FH) and repose angle (α) we e used s ou pu s. Results and Discussion: The fit between experimental data and those provided by ANN were quite satisfactory just after the first training cycles, with percentage error ranging from 2% (FH) to 5% (IC) and no anomalous behavior observed in the error decay curve. Data fitting for α was poor; error percentage was close to 45% with abnormalities in the error decay curve denouncing suffering in learning process. Subsequent adjust of the network resulted in an adjustment of data better than those obtained for IC and FH, with percentage error close to 0%. Conclusions: Low complexity networks with good predictive capacity were obtained. The predictive capacity and the error evaluation parameters indicate an appropriate learning curve for classification of rheological parameters of powders. Financial Support: CAPES/CNPq.



135

CODE 130 GASTROPROTECTIVE EFFECTS OF ETHANOLIC EXTRACT FROM

SPONDIA PURPUREA ON EXPERIMENTAL GASTRIC ULCER MODELS IN RODENTS Dantas, A.M.1*; Silva, K.M.A.1; Coutinho, A.C.1; Santos, V.L.1; Silva Júnior, E.D.2 1 Department of Pharmacy, State University of Paraíba, Brazil. 2 Department of Pharmacology, Federal University of São Paulo, Brazil. Corresponding author: [email protected] Introduction: The Spondia purpurea, Anacardiacea, is a Brazilian plant commonly known as Siriguela. The leaf of this herb has pharmacological properties against several diseases such as gastric ulcer. Objective: Therefore, the aim of this study was to evaluate the effect of ethanolic extract from S. purpurea (EESP) on animal models of human gastric ulcer. Materials and Methods: Adult male Wistar rats and Swiss mice were used. Gastric ulcer was induced in rats by Ethanol (99.6%) and in mice by Piroxican 20mg.Kg-1. The animals were pretreated orally with drug-free vehicle 10mL.Kg-1 (negative control), EESP at doses of 250, 500 and 750mg.Kg-1, Lanzoprazole 30mg.Kg-1 (for Ethanol-induced gastric ulcer in rats) or Cimetidine 20mg.Kg-1 (for Piroxican-induced gastric ulcer in mice). Results and Discussion: Both models of gastric ulcer showed a significant inhibition of the lesions when compared to negative control, expressed as inhibition percentage. Ethanol-induced gastric ulcer in rats was inhibited at about 34.21% by Lanzoprazole. However, EESP was able to inhibit the lesions in 47.04, 53.93 and 74.07%, at doses of 250, 500 and 750mg.Kg-1, respectively. On the other hand, Piroxican-induced gastric ulcer in mice was diminished in 60.45% by pretreatment with Cimetidine. In this model, EESP decrease the gastric lesion levels in 24.29, 20.9 and 60.45%, at doses of 250, 500 and 750mg.Kg-1, respectively. Conclusion: From our data we suppose that ethanolic extract from S. purpurea has a similar antiulcerogenic activity to Lanzoprazole and Cimetidine. Accordingly, we concluded that plant species studied has a gastroprotective effect. Financial support from CNPq/UEPB.



136

CODE 131 IMPACT ON HEMATOLOGICAL PARAMETERS OF L

ARGININE SUPPLEMENTATION IN PATIENTS WITH SICKLE CELL ANEMIA IN USE OF HYDROXYUREA Elias, D.B.D.1; Barbosa, M.C.1; Bandeira, I.C.J.1; Santos, T.E.J.1; Dutra, L.L.A.1; Pedrosa, A.M.1; Silva, H.F.2; Machado, R.P.G.2; Martins, A.M.C.1; Gonçalves, R.P.1

1Laboratory of Hematology, Department of Clinical and Toxicological Analyses, Federal University of Ceará (UFC). 2 Hematology and Hemotherapy Center of Ceará (HEMOCE). Corresponding author: [email protected] Introduction: The sickle cell anemia (SCA) is a chronic hemolytic anemia, characterized by vascular damage, mainly attributed to lower availability of nitric oxide (NO). Hydroxyurea (HU), used in the treatment of AF, induces the synthesis of HbF and acts as NO donor. The erythrocyte arginase released during hemolysis consumes L-arginine, substrate for NO production, compromising the action of HU in inducing HbF. Objective: We assessed the impact of the association of HU with L-arginine supplementation in blood parameters of patients with SCA treated at University Hospital Walter Cantídio. Materials and Methods: A total of 13 patients with SCA in the use of HU were analyzed, as follows: Group I, patients with SCA in the use of HU (> 1g/day) (n = 6) and Group II, patients with SCA in the use of HU (> 1g/day) with L-arginine supplementation (250mg/day) for 12 weeks, which were followed in four stages (0, 4, 8 and 12 weeks) (n = 7). Results and Discussion:We analyzed the mean and standard deviation of Hb, MCV, HbF, WBC,Reticulocytes and lactate dehydrogenase (LDH) of patients in Group II (at 0, 4, 8 and 12 weeks of combined use of L-arginine + HU) n = 7, and we observed a significant increase in the reticulocyte count is a non-significant increase of Hb and HbF. There were no differences in other parameters. Conclusion: The L-arginine supplementation in patients receiving HU induced increase in erythropoiesis, being related to the levels of HbF, suggesting that HU increases HbF levels using nitric oxide pathway.



137

CODE 132 ALTERAÇÕES HEMATOLÓGICAS EM CRIANÇAS COM TETRALOGIA

DE FALLOT SUBMETIDAS À CIRURGIA CARDÍACA COM CIRCULAÇÃO EXTRACORPÓREA Arruda, A.B.L.1; Souza, I.P.1; Gonçalves, R.P.1; Mota, A.M.1; Estevães, B.S.1; Lima, D.M.1; Bandeira, J.A.1; Barbosa, M.C.1, Pedrosa, A.M.1; Bandeira, I.C.J.1

1Laboratory of Hematology, Department of Clinical and Toxicological Analyses, Federal University of Ceará (UFC). Corresponding author: [email protected] Introduction: Tetralogy of Fallot (T4F) is most common form of cyanotic congenital heart disease. During cardiac surgery is used cardiopulmonary bypass (CPB) with heart-lung machine, which can produce haematological changes. Objective: To characterize the main hematological changes induced by cardiopulmonary bypass (CPB) in children with T4F who underwent cardiac surgery at the Hospital de Messejana, in Fortaleza. Materials and Methods: We studied 175 children in pre-and post-operative (PO) immediate heart surgery and 1, 2 and 7 days after surgery. Count of red blood cells were analyzed (He), hemoglobin (Hb), hematocrit (Ht), prothrombin time (PT), activated partial thromboplastin time(APTT) and platelet count. Results and Discussion: Of the 34 children with T4F, 65.0% were female, aged between 5 and 10 years and weighing between 10 and 20 kg. The data analysis showed a decrease in He, Hb and Ht in post-operative as a result of surgical correction of T4F, which caused a reduction of cyanosis and polycythemia and improves the functional capacity of patients. Significant changes were observed in TAP (sec), Activity (%), INR and platelets. As for the APTT (sec), an increase in value after the surgery (POI) and, over time, there was a decrease. Conclusion: The significant decrease in platelet count after surgery demonstrates the ability to differentiate groups of children with cyanotic more or less likely the risk of thrombocytopenia and bleeding in the times studied.



138

CODE 133 PROFILE OF PATIENTS WITH CHRONIC DISEASE NON-

TRANSMISSIBLE PARTICIPANTS OF A PHARMACEUTICAL CARE PROGRAM Rodrigues, T. P.1; Cartaxo, N. A. O.1; Oliveira, A. R.1; Brandão, D. O.1; Silva, P. C. D.1; Silva, F. R.1; Queiroz, M. do S. R.2

1Discente do curso de Farmácia, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 2 Docente da disciplina de Assistência Farmacêutica, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). Corresponding author: [email protected] Introduction: Hypertension and diabetes mellitus are public health problems responsible for cardiovascular events that cause early disability, death and for the public sector increased hospital costs. Objective: Tracing the profile of people with chronic disease non- transmissible. Materials and Methods: This study was conducted from March to June 2009, in the Municipal Health Service, Campina Grande-PB.It was evaluated blood pressure and glucose levels, so the patients were oriented about the disease appears, pharmacotherapy and they received educational folders. All data collection was done through the records of the Pharmaceutical Care Program.The data were evaluated using the Epi-info 3.5. Results and Discussion: Of the 62 participants, most of them were female (64%) and 69% just had hypertension. In the evaluation of metabolic disorders, 65% of the participants had metabolic syndrome, and the blood pressure (BP), central obesity and HDL cholesterol were the main components changed. As for the dyslipidemia types, it were more prevalent hypercholesterolemia associated with hypertriglyceridemia and low HDL. The most used pharmacological groups were diurects and inhibitors of angiotensin I converting enzyme. Conclusions: The practice of pharmaceutical care contributes to the control of blood pressure, bood glucose and metabolic disorders, however its necessary to intensify the actions encouraging the practice of healthy habits that correspond to proper diet and physical activity. Financial support from UEPB/Brazil.



139

CODE 134 PREPARATION AND CHARACTERIZATION OF INCLUSION

COMPLEXES BETWEEN CHLORPROPAMIDE AND β-CYCLODEXTRIN

Dasmasceno, G. A. B.1; Freire, F. D.2; Raffin, F. N.1

1Depto. de Farmácia – CCS - Univ. Federal do Rio Grande do Norte - UFRN, R. General Gustavo Cordeiro de Farias, S/N CEP: 59010 180 Natal (RN) Brasil. 2Pós-graduação em desenvolvimento e inovação tecnológica em medicamentos – PGDITM – UFRN. Campus Universitário – Av. Senador Salgado Filho, 3000 – Lagoa Nova CEP 59.072-970 – Natal (RN) Brasil. Corresponding author: [email protected]

Introduction: Chlropropamide (CLP) is a sulfonylurea acting on pancreas beta cells that are still in activity. On Biopharmaceutics Classification System, CLP is placed in class II because of its high permeability and poor solubility which can lead to decreased efficacy of oral treatment. Inclusion complexes (IC) with cyclodextrins are an interesting strategy to increase the solubility of hydrophobic molecules as CLP. Objective: The aim of this work was to ev u e he effec s of βCD on he pp en so ub y of CLP by the phase solubility technique, comparing IC obtained by different techniques. Materials and Methods: Phase solubility studies were performed in accordance to Higushi and Connors, CLP-βCD complexes were prepared by physical mixture (MP), kneading (KD) and freeze-drying (FD) and analyzed through differential scanning calorimetry (DSC). Solubility was evaluated by the addition of an excess amount of each IC to 5mL of water and the concentration of CLP has been determined by spech opho ome y UV (λ=232nm). Results and Discussion: Phase solubility diagram showed an AL complex (K=175.665M-1). MP, KD and FD showed an increase on the CLP apparent solubility and DSC curves, different levels of in e c on be ween CLP nd βCD fo MP nd KD nd the formation of a true CI for FD. Conclusions: The results showed that ICs are an interesting alternative to the poor CLP solubility which can improve effectiveness of dosage forms used in the treatment of Diabetes mellitus.



140

CODE 135 TOXICOLOGICAL PROFILE OF EMERGENCY ATTENDED AT VIÇOSA-

AL HOSPITAL FERREIRA, D. G.1; MIRANDA, N.1; GOMES, N. S.1; DE MOURA, M. A. B. F.1

1 Laboratório de Biociência Farmacêuticas, Núcleo de Toxicologia. Escola de Enfermagem e Farmácia – UFAL. Corresponding author: [email protected] Introduction: This research aimed to identify cases of toxicological emergencies, reported in 2010 the municipality of Viçosa-AL, for this it is a representative of the central municipality of the state. Materials and Methods: The survey was conducted in Viçosa Municipal Hospital, between January and March 2011 on the period from February to December 2011 were examined medical records of emergency, after evaluation of each record, if it were found some poisoning case, information was collected as sex, age, date, diagnosis and therapy employed, then this information was compiled. Results and Discussions: At the end of the study were identified two hundred fifty-five poisoning cases, among them cases of alcohol poisoning, food, medicinal, poisonous animals typical of the region, and cases of poisoning by disinfectants, pesticides, poisoning volunteers, without apparent cause poisoning, among others. It was noted that monthly were around two thousand to two thousand and five hundred patients. Typically, cases of poisoning were in the hospital care, only in really severe cases, is that the patient was referred to the capital. Conclusion: Through this survey it was concluded that the cases are the higher incidence of food poisoning, alcohol poisoning and scorpion bites, respectively. It was also observed that there was no specific toxicological analysis to identify the possible toxicant involved in poisoning. Was also suggested an underreporting of cases of poisoning. Financial support from FAPEAL.



141

CODE 136 DEVELOPMENT AND VALIDATION OF HPLC FAST METHOD FOR

SIMULTANEOUS DETERMINATION OF NINE POLYPHENOLS COMPOUNDS IN CRUDE EXTRACTS OF PLANTS Araujo-Silva, G.1; Solon, L.G.S.2; Santos, R. N. P.1; Morais, L. V. F.1; López, J. A. R.1; Almeida, M. G.1

1LABMULT, Department of Clinical and Toxicological Analyses - UFRN/Natal-RN/Brazil. 2Quality Control of Drugs Laboratory, Department of Pharmacy- UFRN/Natal-RN/Brazil.

Corresponding author: [email protected] Introduction: A development and validation assay methods is an important part of the quality control of natural products, and such assays are required by Brazilian health authorities for registration of phytomedicines. Objective: The aim of this work was describe a fast chromatographic method for determination of polyphenolic compounds in crude extract of plants from Northeast of Brazil. Methods: A reversed-phase mode in a C18 column (100 x 4.6 mm id, particle size 2.6 µm) and UV absorption spectroscopy. The elution gradient was generated from 0.1% formic acid aqueous solution and acetonitrile as an organic modifier. Experimental conditions including pH, percentage of organic modifier and elution gradient profile have been thoroughly optimized. Linearity ranges, precisions, detection limits and stability have been established under selected experimental conditions using synthetic standards and extract solutions. Results and discussion: The use of an analytical column packed with 2.6 mm particles has contributed to achieve an efficient separation of analytes in less than 20 min. The use of the experimental design and the multicriteria approach has facilitated the optimization of the separation to reach the best compromise among resolution of close peaks and analysis time in a limited number of assays. Conclusion: The method has been applied to analyze crude extracts of plants from Northeast of Brazil, according to Brazilian legislation (RE 899); this seems to be a significant advantage with respect to other methods described in the literature for similar analyses. Finnantial supported from CNPq/Brazil.



142

CODE 137.1

APPLICATION OF DIFFERENTIAL THERMAL ANALYSIS (DTA) TO DETERMINE OF TRIOXSALEN-EXCIPIENT COMPATIBILITY Barros, D. M. C.2, Lima, N. G. P. B.1,2; Lima, I. P. B.2; Oliveira T. S.2; Medeiros, A. C. D.1; Gomes, A. P. B.2; Aragão, C. F. S.2

1 Department of Pharmacy, Stadual University of Paraiba – UEPB. 2Laboratory of Quality Control , Department of Pharmacy, Federal University of Rio Grande do Norte UFRN, Brazil. Corresponding author: [email protected]

Introduction: Trioxsalen plus ultraviolet (UVA) and photochemotherapy (PUVA) is frequently used in psoriasis and other dermatoses (Edward; Jeffrey, Laskin, 1991). Objective: The purpose of the present work was to study the compatibility of trioxsalen (TX) with different pharmaceutical excipients using differential thermal analysis (DTA). The samples were analyzed by TG/DTA. Materials and Methods: The curves were obtained on a SHIMADZU thermobalance, using an alumina crucible, at heating speed of 10ºC.min-1, on a temperature interval of 35 – 900ºC, under an atmosphere of nitrogen at 50 mL.min-1. Sample weight was around 8 mg. The mixtures in a 1:1 (w/w) were prepared by mixing the drug:excipient. The excipients used were magnesium stearate (MS), colloidal silicon dioxide

(CSD - aerosil®), lactose monohydrate (-lactose), microcrystalline cellulose

PH 102 (MC – 102), pregelatinized starch (starch), mannitol, sodium lauryl sulfate (SLS), sodium starch glycolate (SSG) and croscarmellose sodium (CCS). Results and Discussions: The DTA curve of trioxsalen present a

sharp endothermic event at 234oC indicating the melting and which corresponds to the values indicated in literature (234-235oC). The adjuvant that showed interactions was SLS used in a 1:1 ratio. These results indicated the occurrence of a strong interaction in the solid state with temperature, but not necessarily corresponding to incompatibility. Conclusion: The compatibility of trioxsalen with various pharmaceutical excipients could be tracked by DTA measurements. The SLS presented thermal interactions with the TX. Financial Support from PROPESQ-REUNI-UFRN, UEPB, CNPq, CAPES and FAPERN.



143

CODE 137.2 COMPATIBILITY STUDY OF VITAMIN D3 AND MANNITOL IN

PHYSICAL MIXTURES Barros, D. M. C.2; Lima, N. G. P. B.1,2; Lima, I. P. B.2; Fernandes, F. H. A.1; Correia, L. P.1; Medeiros, A. C. D.1; Aragão, C. F. S.2

1 Department of Pharmacy, Stadual University of Paraiba – UEPB. 2 Laboratory of Quality Control, Department of Pharmacy, Federal University of Rio Grande do Norte UFRN, Brazil. Corresponding author: [email protected] Introduction: Vitamin D, the sunshine vitamin, is now recognized not only for its importance in promoting bone health in children and adults but also for other health benefits, including reducing the risk of chronic diseases such as autoimmune diseases, common cancer, and cardiovascular disease (Edward; Jeffrey, Laskin, 1991). Objective: The purpose of the

present work was to study the compatibility of Vitamin D3 with mannitol using differential scanning calorimetry (DSC). Materials and Methods: The

DSC curves were obtained using a DSC Q20 (TA instrument) under dynamic nitrogen atmosphere with the flow rate of 50 mLmin-1. 2 mg of samples were weighted out and placed in a sealed aluminum pan. The analysis was carried out from 25 - 450 ºC at a heating rate of 10ºC min-1. The mixtures in a 1:1 (w/w) were prepared by mixing the drug:excipient. Results and Discussions: The DSC curve of vitamin D3 present a sharp endo he m c even 85 ◦C nd c ng he me ng nd wh ch co esponds o the values indicated in literature (84-85 ◦C). A sharp melting endotherm was observed at 167 ◦C in the DSC of mannitol. DSC curves of vitamin D3-mannitol mixture showed an endothermic peak of drug at 85 °C, indicating that there was no interaction. DSC data showed compatibility of vitamin D3 with diluent mannitol. Conclusion: However, further studies should be

performed such as FTIR and SEM to confirm the findings. Financial Support from PROPESQ-REUNI-UFRN, UEPB, CNPq, CAPES and FAPERN .



144

CODE 138 A QUANTUM STUDY OF DRUG-RECEPTOR INTERACTION:

RECONHECENDO AS BINDING ENERGIES BETWEEN 16S rRNA RESIDUES AND HYGROMYCIN B Oliveira, J. I. N.1; Fulco, U. L.1; Albuquerque, E. L.1; Freire, V. N.2; Neto, J. X. L.1 1 Laboratory of Biophysics and Computational Simulation, Department of Biophysics and Pharmacology, at Federal University of Rio Grande do Norte, 59072-970 Natal, RN, Brazil. 2 Department of Physics at Federal University of Fortaleza, 60455-760, Fortaleza, CE, Brazil. Corresponding author: [email protected] Introduction: The Hygromycin B (HygB) is an aminoglycoside initially isolated from Streptomyces hygroscopicus, and as such inhibits the bacterial protein synthesis through irreversible binding to the 16S ribosomal RNA (16S rRNA) of the bacterial 30S ribosomal subunit (30S). Objective: Due to strong electrostatic character of this connection, we propose an energetic investigation of binding mechanism of this complex using dielectric constants, which have been widely used to study the electrostatic properties of biomolecules. Materials and Methods: For this, we calculated the drug-binding energy of each nucleotide inside the Hygromycin B recognition site on 30S bacteriam ribossome by using the molecular fractionation with conjugate caps (MFCC) strategy. Results and Discussion: We saw that high E values underestimate the individual interaction energy between HygB and each 16SrRNA nucleotide of binding site, and quickly enable the convergence state of the total binding energy. From an efficient process of convergence, we define a suitable binding pocket encompassing all major residues able to stabilize the Hyg B in the 30S ribosome. Thus, identify that C1403, C1404, G1405, A1493, G1494, U1495, C1496 and U1498 are the residues that interact more strongly with the drug. These residues probavly lead to a state of drug resistance in bacteria when they suffer single base substitutuions, whereas those with low interaction energy probably will not effectively influence the affinity of the binding pocket Hygromycin B and therefore would not generate resistance when mutated. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil).



145

CODE 140 STUDY OF 599 C>T POLYMORPHISM OF THE GPX1 GENE IN TYPE 1

DIABETES: IMPLICATIONS IN ANTIOXIDANT DEFENSE AND DIABETIC NEPHROPATHY Oliveira, Y. M. C.1; Souza, K. S. C.1; Silva, H.P.V.1; Ururahy, M. A. G.1; Loureiro, M. B.1; Oliveira, G. H. M.1; Morais, L. V. F.1; Arrais, R. F.2; Abdalla, D. S. P.3; Hirata, R. D. C.3; Hirata, M. H.3; Rezende, A. A.1; Almeida, M. G.1

1 Department of Clinical and Toxicology Analyses - UFRN/Natal-RN/Brazil. 2 Department of Pediatrics- UFRN/Natal-RN/Brazil. 3 Department of Clinical and Toxicology Analyses- USP/São Paulo-SP-Brazil. Corresponding author: [email protected] Introduction: Relationship between reactive oxygen species (ROS) and diabetes is well established in literature. Genetic variations in antioxidants enzymes such glutathione peroxidase (GPX) can result in reduction of their antioxidant potential and predispose to development of diabetic nephropathy (DN). Objective: Evaluate the influence of GPX1 599 C>T polymorphism in erythrocyte activity of GPX and GSH content and their implications in diabetic nephropathy in DM1 patients assisted at a pediatric hospital (HOSPED/UFRN) in Natal-RN/Brazil. Methods: 77 DM1 patients and 91 normoglycemic subjects (NG) between 6 and 20 years were recruited. GPX1 (599 C>T) polymorphism was determined by allelic discrimination technique in real-time PCR. GSH content was quantified as described by Beutler. Erythrocity GPX activity was determined using commercial kits by Randox®. Albumin/creatinine (RAC) ratio was determined using kits by Biosystems®. Results and discussion: The content of GSH was significantly lower in DM1 patients comparing NG subjects (p=0,024). The red cell GPX activity was significantly higher in DM1 group (p= 0,001). Eleven DM1 patients showed RAC> 30 µg/mg characterizing microalbuminuria recognized as early sign for detection of DN. The presence of the 599T allele was associated with a decrease GSH content (p= 0,002) and lower erythrocyte GPX activity (p=0,001) in DM1 patients, leaving them more vulnerable to oxidative damage. Conclusion: These findings suggest a deleterious role of the 599T allele (GPX1) in antioxidant defense of patients with DM1 leading to accumulation of ROS which could contribute to the onset of diabetic nephropathy evidenced by elevated RAC in patients studieds. Financial supported from CNPq/Brazil.




146

CODE 141.1 PRELIMINARY STUDY FOR THERMAL CHARACTERIZATION OF

BIOIDENTICAL HORMONE ESTRADIOL Pereira, T. M. M.2; Silva, M. M. C.1; Penha, S. L. T.1; Gomes, A. P. B.3; Aragão, C. F. S.3 1Undergraduate, Quality Control of Drugs Laboratory (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Posgraduate LCQMed, DFAR, UFRN. 3 Professor, LCQMed, DFAR, UFRN, Brazil. Corresponding author: [email protected] Introduction: Bioidentical hormones are compounds that have exactly the same chemical and molecular structure as endogenous human hormones. Health care providers often prescribe hormones for menopausal women symptoms. Studies about thermal behavior of Bioidentical Hormones using thermal techniques are scarce in the literature. Objective: This study aims

to thermal characterization, through curves TG / DTA, to get the best event for the quantification Estradiol. Materials and Methods: Bioidentical Hormone Estradiol was analyzed by TG/DTA. The curves were obtained on a SHIMADZU thermobalance, model TG/DTA, using an alumina crucible, at heating speed of 20ºC/ min–1, on a temperature interval of 25 – 900ºC, under an atmosphere of nitrogen at 50 mL/ min–1. Sample weight were around 0.1; 0.2; 0.5; 1; 2; 4; 6; 8; and 10 mg. Results and Discussion: DTA curves of Bioidentical Hormone Estradiol, present four

events and TG curve only one thermo decomposition processes. Thermal data of the Bioidentical Hormone Estradiol supply important information on evaluation of stability of the materials, becoming an important tool on quality control and pharmaceutical technology of on the development of products. The Thermal event B showed the best Linearity correlation (R= -0,99984) in DTA method between four events. Conclusions: TG/DTA study of Bioidentical Hormone Estradiol show your thermal stability, and this parameters can be used in the development and characterization of pharmaceutical products. Financial Support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN.



147

CODE 141.2 LINEARITY IN DTA METHOD: A COMPARISON OF TWO

QUANTIFICATION STRATEGIES OF BIOIDENTICAL HORMONE Pereira, T. M. M.2; Silva, M. M. C.1; Penha, S. L. T. 1; Gomes, A. P. B.3; Aragão, C. F. S.3 1Undergraduate, Quality Control of Drugs Laboratory (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Posgraduate LCQMed, DFAR, UFRN. 3 Professor, LCQMed, DFAR, UFRN, Brazil. Corresponding author: [email protected] Introduction: Bioidentical Hormone Replacement Therapy describes supplementation of hormones that are molecularly identical to those hormones produced in the human body such as Estriol Bioidentical. TG–DTA/DSC is the quick and convenient techniques and has been widely used to characterize the changes of mass and enthalpy of materials during the thermal decomposition. Objective: Conduct the study of thermal characterization through DTA curves in order to get the best event for quantification of Bioidentical Estriol. Materials and Methods: It was used Estriol drug. Non-isothermal thermogravimetric curves of Estriol drug were obtained using a Shimadzu model DTG-60 thermobalance at 20°C.min–1 heating rates up to 900°C in a nitrogen (flow rate: 50 mL.min–1). The curves were obtained on several of initial sample mass (0.1, 0.2, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and 10.0 mg) in alumina crucible. Results and Discussion: In DTA curves was observed two events, fusion (from 280 to 315ºC) and degradation (from 350 to 430ºC). The Thermal event A (fusion) showed the best Linearity correlation (R= -0,99965) in DTA method that the event B (degradation). Conclusions: The linearity in DTA method showed endothermic event A could be used between 0.142 and 10.041 mg to quantification of pharmaceutics products with Bioidentical Hormone Estriol. Financial Support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN



148

CODE 142 INTERFERENCE ON ADHERENCE OF Candida ssp IN CONTACT

LENSES BY MICROEMULSION SYSTEMS Silveira, W. L. L.1; Silva, K. S.1; Ferreira, L. F.1; Farias, M. G. I.2; Shinobu, C. S.2; Svidzinski, T. I. E.2; Egito, E. S. T.1*

1 Laboratório de Sistemas Dispersos, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 2 Laboratório de Micologia, Universidade Estadual de Maringá (UEM). Corresponding author: [email protected] Introduction: Biofilms represents the most prevalent type of microbial growth in nature. Investigations of fungal biofilms associated with contact lenses are virtually nonexistent and the lack of robust in vitro models has resulted in scant information about the pathogenesis of Candida keratitis. Objectives: The aim of this work was to study the interference on the adherence, first step for biofilm shaping by Candida strains, in contact lenses, submitted to the exposition of a microemulsion system (ME). Materials and Methods: Isolated and ATCC yeast of Candida albicans and Candida tropicalis were incubated at 25°C ± 2.5 over 24 hours. Fungal cells were suspended, after the growth, and prepared at the concentration of 102 cfu in saline solution (0.85%) or ME samples to guarantee their viability. Four hours later the cells were inserted in 24 well plates containing the contact lenses. To evaluate the fungal adherence capacity, the Shin´s method was adapted and used. Results and Discussion: Compared to the control, the ME completely impeached the adherence of the strains. The interference induced by the ME on the biofilms formation was probably due to the surfactants on the formulation, which disturbers the cell membrane causing changes in the biofilms shaping. Conclusions: The biofilm formation by Candida spp strains were inhibited by the influence of the ME. The surfactants of the ME composition may be the main reason of this interference because of they are able to compromise the stability of the lipids contained in the membrane cells, turning them unstable. Financial Support from CNPq/Brazil and Procad NF 2008/ Capes.



149

CODE 143.1 CHARACTERIZATION OF THERMAL EVENTS OF HYDROQUINONE

DEPIGMENTANT BY DTA Oliveira, T.S.1; Barros, D.M.C.1; Lima, I.P.B.2; Lima, N.G.P.B.2; FERRARI, M.3; Aragão, C.F.S.3* 1 Undergraduate, 2Postgraduate and 3Professor, Federal University of Rio Grande do Norte – UFRN, Brazil. Corresponding author: [email protected] Introduction: The depigmentant drug mostly used in the treatment of melasma is hydroquinone (HQ)[1]. Materials and Methods: Differential thermal analysis (DTA) has been used for characterization of the materials[2]. The aim of this work was to characterize the thermal events of hydroquinone by DTA. The HQ was analyzed by TG/DTA, on a SHIMADZU thermobalance, in the 25-900°C temperature range, under an atmosphere of nitrogen, at heating speed of 10ºC min-1 with different sample weights of 3, 5 and 7 mg. The DTA curves were used to identify thermal events as well as the temperatures and energies involved in these events. Results and Discussions: DTA curves present two thermal decomposition events and shows that for the sample weight of 3mg the first event happens at Tonset of 172°C, Tpeak 175°C, Tendset 182°C, 1180 J/g, 282 Cal/g and the second at Tonset 192°C, Tpeak 205°C, Tendset 212°C, 1307 J/g, 312 Cal/g. For the weight of 5mg the first event at Tonset of 172°C, Tpeak 175°C, Tendset 183°C, 1162 J/g, 277 Cal/g and the second at Tonset 195°C, Tpeak 212°C, Tendset 221°C, 1664 J/g, 397 Cal/g. For the weight of 7mg the first event at Tonset of 173°C, Tpeak

176°C, Tendset 185°C, 1270 J/g, 303 Cal/g and the second at Tonset 196°C, Tpeak 219°C, Tendset 229°C, 1984 J/g, 473 Cal/g. The results show good correlation between the thermal events and the change in sample weights. Conclusion: DTA can be useful in the characterization of thermal events

of pharmaceutical materials. FAPERN, CNPq and CAPES by financial support. .



150

CODE 143.2 COMPATIBILITY STUDY OF TRETINOIN AND EMULSIFYING AGENT

IN PHYSICAL MIXTURES Oliveira, T. S.; Barros, D. M. C.; Lima, I. P. B.; Lima, N. G. P. B.; Ferrari, M.; Aragão, C. F. S. Laboratory of Quality Control, Department of Pharmacy, Federal University of Rio Grande do Norte UFRN, Brazil. Corresponding author: [email protected] Introduction: Tretinoin has been used in the management of skin disorder

to improve the appearance and texture of skin. Topically, tretinoin has been used for treatment of ichtyosis, psoriasis, acne vulgaris, neoplasia and other skin disease at various dosage forms, such as solutions, lotions ointments and creams. (Shin et al., 2005). Objective: The aim of the present work was to perform the compatibility study between tretinoin and cetostearyl alcohol using differential thermal analysis (DTA). Materials and Methods: The samples were analyzed by TG/DTA. The curves were

obtained on a SHIMADZU thermobalance, using an alumina crucible, at heating speed of 10ºC.min-1, on a temperature interval of 35 – 900ºC, under an atmosphere of nitrogen at 50 mL.min-1. The mixtures in a 1:1 (w/w) were prepared by mixing the drug:excipient. Results and Discussions: The DTA curve of tretinoin present a sharp endothermic event at 184oC indicating the melting. A sharp melting endotherm was observed at 54oC in the DTA of cetostearyl alcohol. The result of tretinoin in the presence of cetostearyl alcohol was shifting in tretinoin-cetostearyl alcohol mixture, which suggested incompatibility. DTA data showed incompatibility of tretinoin with emulsifying agent. Conclusion: In this case, further studies will be

performed with other tecnhiques, in order to gain insight into the nature of the interaction and to estabilsh an actual pharmaceutical. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN



151

CODE 144 EVALUATION OF THE ANTINOCICEPTIVE EFFECT OF

HYDROETANOLIC EXTRACT OF Portulaca pilosa L (PORTULACACEAE) in mice Alexopulos, F. F.1; Melo, A. S.1; Dolabela, M. F.1; Sousa, P. J. C.1

1 Laboratory of Pharmacodynamics, Faculty of Pharmacy/ Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, UFPA. Introduction: The specie Portulaca pilosa belong the family Portulacaceae and genus Portulaca. Has wide distribution in northern Brazil and is popu y c ed “ mo -c esc do”. Objective: To evaluate the antinociceptive effects of the of hydroetanolic extract of Portulaca pilosa L.( HEPp) in mice. Materials and Methods: In the writhing test the animals were pre-treated orally 1 h before to the acetic acid 0,6% with doses of 50, 100, 200 and 400 mg / kg, vehicle and indometacin (5 mg/Kg).The numbers of contortions was recorded for a period of 20 min. The Hot plate test was used to evaluate the effect of the extract (200 e 400 mg/Kg) and morphine (10 mg/Kg) on nociception centrally mediated.The formalin test was assayed to study the effects of the extract (100, 200 and 400 mg/Kg) on the nociception of neurogenic and inflammatory origin. To investigate the opiod receptors involvement was administered the opioid antagonist naloxone before the extract and morphine. Results: The doses of 100, 200 and 400 mg/kg inhibited significantly the abdominals contortions when compared to control group. In hot plate test, the extract no change the latency when compared to control, unlike morphine. In formalin test, the doses of 200 e 400 mg/kg orally significantly inhibited the 1st and 2nd phase of the assay. In addition, the opioid antagonist naloxone reversed the effect of the HEPp in 1st and 2nd phases. Conclusions: The results show that the HEPp orally exerts antinociceptive effect on nociception of neurogenic and inflammatory origin and opioids receptors are involved in this effects. Finnacial Support from FAPESPA/Brazil and master scholarship from CAPES /Brazil.



152

CODE 145 EVALUATION OF PHYSICOCHEMICAL PROPERTIES OF POORLY

WATER SOLUBLE DRUG (SIMVASTATIN) OBTAINED BY RECRYSTALLIZATION Vargas, M. R. W.1, Lima Neto, S. A.2, Torres, S. M.3, Moura, T. F. A. L.1

1 Laboratory of Medicine Development – Health Sciences Center /UFRN, Natal, Brazil. 2 Unified Laboratory of Development and Testing Medicines - Health Sciences Center/ UFPB, João Pessoa, Brazil. 3 Department of Mechanical Technology, Technology Center/UFPB, João Pessoa, Brazil. Corresponding author: [email protected] Introduction: The recrystallization of drugs is one of several strategies to increase the solubility of Drug Class II (Biopharmaceutics Classification System). Simvastatin is one this type of drug of such class that have low water solubility as well as high intestinal permeability. However, the formation of one or more simvastatin´s polymorph during the process of its production can consequently change both physical and chemical properties. Objective: To evaluate the physical and chemical properties of simvastatin obtained by recrystallization with organic solvents. Materials and Methods: Simvastatin was solubilized in different solvents (dichloromethane, ethyl acetate, acetone, methanol). The samples were dried in a rotary evaporator under reduced pressure, placed in a desiccator and the new crystalline forms were evaluated by Infrared Spectroscopy and X-ray powder diffraction (XRD). The results were compared with simvastatin raw material (negative control). Results and Discussion: The infrared spectra of the samples showed bands similar to the controls, suggesting that there was not physical-chemical alteration of simvastatin during the recrystallization. The XRD patterns of the same samples presented similar peaks then the raw material, demonstrating that there was no change in crystallinity and, consequently, no formation of polymorphs. Conclusions: After recrystallization of the drug, the formation of amorphous material was not observed. This observation might lead to an improved solubility. Also, there was no change in the physicochemical characteristics of the drug which gives evidence to its stability. Financial support from CAPES/Brazil.



153

CODE 146 ANALYSIS AND CHARACTERIZATION OF OILS AND FATS

Silva, E. I. L.1; Valença, R. C. A.1; Gurgel, R. K. C.1; Oliveira, R. A.1; Pagnoncelli, M. G. B.1; Assis, C. F.1

1 Laboratory Bromatology, Department of Pharmacy, Universidade Federal do Rio Grande do Norte. Corresponding author: [email protected] Introduction: Lipids are macromolecules of biological importance that can be synthesized in the organism except the essential fatty acids. They can be found in solid form (fat) or liquid (oil). Transformations that can occur in lipids affect its organoleptic qualities, it may cause toxic effects due to intake of these foods. Objective: Check the quality of samples of oils marketed by checking if they are within the standards of identity and quality of Brazilian legislation. Methods: In the analysis we used five samples of soybean oil called AO1, AO2, AO3, AO4 and AO5, these were subjected to four tests: index of saponification, index iodine, index acid and index peroxide. Results and Discussion: The tests were performed for five samples and the values above the reference results were: 40% for the index of saponification, 20% for iodine, 20% for the index of free acidity and 20% for the peroxide. Two tests showed results below the reference values: 20% for iodine and 20% for the rate of saponification. The results show that the changes can be found from the improper storage causing changes in reference values. Moreover, the difference in average weight of fatty acids can occur due to tampering with other types of oil. Conclusions: The samples showed mostly favorable results being adequate for consumption. However some samples need further investigation, due to the unsatisfactory results obtained, for this new analysis can be performed with oils from the same batch for a proof.



154

CODE 148 EVALUATION OF ANTIOXIDANT ACTIVITY IN SEASONAL STUDY OF

PHASE ETHYL ACETATE Bauhinia cheilantha (Bong.) Steudel Oliveira, A. M. F.1; Nóbrega, F. F. F.2; Pereira, C. K. S.1; Penha, A. R. S.1; Almeida, R. N.1,2; Assis, T.S.3

1Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba (UFPB). 2 Programa de Pós-Graduação em Biotecnologia – Rede Nordeste de Biotecnologia (RENORBIO). 3 Departamento de Fisiologia e Patologia, Universidade Federal da Paraíba (UFPB). Corresponding author: [email protected] Introduction: Reactive oxygen species (ROS), derived from the metabolism of oxygen, molecules react with circulating molecules, being essential in many biological systems. Excess ROS may contribute to various diseases such as diabetes, cancer. Natural antioxidants can increase the body's natural resistance, are important as defense mechanisms of cells. The seasonal variation interferes with the production of antioxidants substance. The leaves, stems and roots of Bauhinia species are used for the treatment of diseases such as diabetes. Objective: To determine the total phenolic content and evaluate the antioxidant activity of Bauhinia cheilantha (Bong.) Steudel, collected in four different seasons (col1, col2, col3, col4). Materials and Methods: The total phenolic content was determined using the Folin Ciocalteau. The antioxidant activity was determined using the DPPH radical, according to the method of Silva et al. (2006). Results and discussion: The col4 had the highest content of phenolic compounds (78.22 ± 0.847 mg / EAG g), as well as the best antioxidant activity (3.253 ± 0.231 mg / mL) than the other collections of the plant, with a good correlation between phenolic content and antioxidant activity. Conclusion: The total phenolic content may be responsible for the antioxidant activity of this species, moreover, that the seasonal variation interferes with the production of secondary metabolites such as phenolic substances. The antioxidant activity of plants depends on growing conditions, soil conditions and water. Financial support from CNPq/Brazil.



155

CODE 149.1 PHARMACEUTICAL ASSISTANCE THE ELDERLY: SHARES OF

COMMUNITY INTERVENTION IN BRASILIA TEIMOSA Valença, R. C. A.1; Lourenço, E. I. L.1; Gurgel, R. K. C.1; Oliveira, R. A.1;

Santos, K. C. L.1; Oliveira, A. R. A.2; Rezende, A. A.2 1Graduate Student of Pharmacy, Universidade Federal do Rio Grande do Norte (UFRN). 2Laboratory of Biochemistry Clinical, Department of Clinical and Toxicological, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: The pharmaceutical assistance aims to help ensure that patients derive maximum benefit in the use of their drugs. Objective: Develop pharmaceutical assistance activities in the community of Brasilia Teimosa, municipality of Natal, in order to improve the response to drug treatment and quality of life of elderly hypertensives and diabetics. Materials and Methods: We analyzed 24 elderly for a period of six months. They underwent a questionnaire relating to patient health, the weekly visits to check blood pressure and monitoring of medication, as well as lectures on generic drugs and tools to improve adherence to treatment. Results and Discussion: With the analysis of questionnaires, it was determined that 24 of the patients, 50% (12/24) have diabetes and hypertension, 45.83% (11/24) hypertension and 4.17% (1 / 24) diabetes. After the intervention was a significant improvement in blood pressure of 33.3% (8 / 24) elderly. One of the tools used to improve adherence to treatment was the distribution of kits for the correct storage of medication, 45.83% (11/24) of patients received 41.66% (10/24) already had and 12.5 % (3 / 24) will receive it. Through weekly visits it was found that 75% used medication correctly and 21% joined after the intervention, moreover, about 33.3% (8 / 24) came to use generic drugs after the intervention. Conclusions: The patients treated had a significant increase from the therapeutic response, as detected by checking blood pressure. It is observed that the improvement in quality of life of elderly may be related to pharmaceutical assistance.



156

CODE 149.2 PROTEIN ANALYSIS BY BRADFORD METHOD IN SAMPLES OF

CEREAL Valença, R. C. A.1; Gurgel, R. K. C.1; Silva, E. I. L.1; Oliveira, R. A.1; Pagnoncelli, M. G. B.1; Assis, C. F.1

1 Laboratory Bromatology, Department of Pharmacy, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Proteins are essential for the proper functioning of the organism, exercising the most varied functions. There are many methods for the determination of proteins, among them there is the method of Bradford, based on the interaction of the dye BG-250 with high molecular weight proteins with basic or aromatic side chains. Objective: To determine the total protein concentration in samples of cereals. Methodology: A total of five samples, two of wheat bran (A1 and A2), an oat (A3) and two of corn flour (A4 and A5). In the first part of the method was performed chemical extraction of proteins. In the second step the samples were diluted in four different concentrations. In third was added the Bradford reagent, with the subsequent reading of samples in the UV visible spectrophotometer at a wavelength of 595 nm. To find the concentration of protein was used a standard curve of albumin. Results and Discussion: For samples A1 and A2 was obtained a value of 0,6 g of proteins for 100 g of wheat bran. The sample A3, presented 0,3g proteins/100g of oats. Sample A4 0,4 g proteins/100g presented corn flour. Already the sample A5 showed a result of 0,2g proteins/100g of the sample. Conclusion: In the observed data, it is verified that the cereals are not direct sources of proteins, but when coupled with a balanced diet can contribute to the daily intake of this nutrient, mainly wheat bran with the highest protein concentration.



157

CODE 150 ANTIMICROBIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF

Stryphnodendron adstringens Mart. AGAINST STRAIN OF Staphylococcus aureus Araújo, G. M. F.1; Almeida, C. L. F.1; Araújo, J. S. C.1; Ramos, K. R. L. P.1; Eleamen, G. R. A.1; Catão, R. M. R.2

1 Pharmaceutical Sciences Graduate Program of State University of Paraiba (UEPB). 2 Center of Biological and Health Sciences, Pharmacy Department, State University of Paraiba (UEPB). Corresponding author: [email protected] Introduçao: Stryphnodendron adstringens Mart. Leguminosae-Mimosaceae, conhecido como barbatimão são amplamente utilizadas na medicina popular para o tratamento de diarréias, problemas ginecológicos e como a cicatrização de feridas. Estudos anteriores mostraram significativas propriedades cicatrizante, atividades anti-inflamatórias e antimicrobianas. Objetivo: Os objetivos deste es udo fo m v “ n v o” v d de n m c ob n e Concen ção In b ó Mín m (MIC) do extrato hidroalcóolico de S. adstringens Mart. frente a cepas padrões. Materiais e Métodos: Os microorganismos testados foram Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853). O estudo foi realizado pelo método de difusão em disco (CLSI, 2005) d c on do 20μL do extrato em discos de papel filtro estéril (6mm de diâmetro), previamente identificados. As placas foram incubadas a 37 º C por 24h e posteriormente a leitura foi realizada. Foi considerado ativo os halos de inibição do crescimento com diâmetro superior a 8 mm. Resultados e Discussão: a atividade antibacteriana do barbatimão foi observada frente a S. aureus, e para as demais cepas não foi observado a atividade. Para a cepa de S. aureus, o barbatimão foi ativo até a concentração de 12,5%. Conclusões: O barbatimão apresentou-se ativo nas condições analisadas apenas frente a cepa de S. aureus. Financial support from CAPES / Brasil.



158

CODE 151 STUDIES OF MAGNETIC PARTICLES PREPARED BY CO-

PRECIPITATION UNDER MAGNETIC FIELD FOR DRUG DELIVERY Silva, K. L.1; Freitas, E. L. S.1; Pontes, T. R. F.1; Araujo Neto, R. P.1; Carvalho, J. F.1; Egito, E. S. T.1; Torres, M. M.2; Carriço, A. S.3

1 Laboratório de Sistemas Dispersos (LASID), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 2 Departamento de Ciências exatas e Naturais, Universidade Federal Rural do Semiárido. 3 Departamento de Física Teórica e Experimental, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Magnetic particles have been increasingly used in biotechnology. Most applications, such as hyperthermia and magnetic drug targeting, benefit from large values of the initial susceptibility and saturation magnetization. Growing magnetite micro-particles by co-precipitation involves the formation of small nano-crystallites and their aggregation. It is thus possible to interfere in the magnetic structure of the micro-particles. Objective: Tailoring the nano-structure of magnetite micro-particles, particularly the relative orientation and size of nano-crystals, to obtain particles with improved properties for drug targeting. Materials and Methods: Magnetite particles were prepared by chemical co-precipitation method of ferrous chloride and ferrous sulphate (2:1) with NaOH in both the presence and absence of external magnetic field (MF), generated from a pair of Nd-magnets. Moreover, the samples were analyzed by Mossbauer Spectroscopy (MS) and Vibrating Sample Magnetometry (VSM). Results and Discussion: MS and VSM revealed that the samples are superparamagnetic. Also, the synthesis in the presence of an external MF of 172mT favored an increase in the initial susceptibility of around 25%. The saturation magnetization showed a larger increase (44%), from 40.27emu/g, for the control sample, to 59emu/g for the one grown in the presence of a 172mT MF. Conclusions: It is possible to tailor the initial susceptibility and the saturation magnetization of magnetite micro-particles grown by co-precipitation by using an external MF during the synthesis. The simple and easy MF assisted co-precipitation method may turn out to be useful for preparing magnetic micro-particles for a wide range of biomedical applications. Financial support from CNPq/Brazil and CAPES/Brazil.



159

CODE 152 A STRUCTURAL AND ENERGETIC INVESTIGATION OF

PAROMOMYCIN-16S rRNA COMPLEX Azevedo, J. L. C.1; Fulco, U. L.1; Albuquerque, E. L.1; Oliveira, J. I. N.1; Costa, A. H. L.1

1 Laboratory of Biophysics and Computational Simulation, Department of Biophysics and Pharmacology, at Federal University of Rio Grande do Norte, 59072-970 Natal, RN, Brazil. Corresponding author: [email protected] Introduction: The Paromomycin is an aminoglycoside antibiotic, which binds to the 30S ribosomal subunit and promotes the inhibition of protein synthesis by reducing the dissociation rate of tRNA site. From the binding energy of the drug in the ribosome, it is possible to see which nucleotides have the biggest interaction and which of them influence the effectiveness of this connection. Objective: In this work, we implement a full quantum mechanical calculation in the three-dimensional structure of 30S ribossome subunit from T. thermophilus in contact with Paromomycin, and calculate the 30S–Paromomycin binding energy to understand the details of the drug interaction with their macromolecular target. Materials and Methods: Using the Molecular Fractionation with Conjugate Caps (MFCC) method, we calculate the binding energies of each individual nucleotide encompassed within 16Å sphere centered on the drug centroid. Results and Discussion: We found A1408 (-18,3 Kcal/mol), G1491 (-16,9 Kcal/mol), G1405 (-15,4 Kcal/mol), U1495 (-14,7 Kcal/mol), G1494 (-11,8 Kcal/mol), C1407 (-11,2 Kcal/mol) and G1405 (-9,8 Kcal/mol) nucleotides interacting strongly with the drug and, consequently, are indispensable for drug-30S stability. Experimental data show the phenotype of drug resistance of bacteria with single point mutations in these residues. The present quantum s udy p ov des mpo n ns gh on spec fic bosome–drug interaction and should be quite useful in the design of new m nog ycos des h h ve “supe s ong” b nd ngs o he 30S. Financial support from CNPq/Brazil.



160

CODE 153 SYNTHESIS AND CHARACTERIZATION OF AMOXICILLIN AND

METRONIDAZOLE-BASED CHITOSAN MICROPARTICLES Carvalho, J.R.P.1; Freire, F.D.1,2, Morais, W.A.3; Raffin, F. N.1,2

1Universidade Federal do Rio Grande do Norte, Departamento de Farmácia, Laboratório de Desenvolvimento de Medicamentos (LDM) – Natal, RN. 2Programa de Pós Graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos (PPgDITM) – UFRN. 3Univesidade Federal do Rio Grande do Norte, Departamento de Farmácia, Laboratório de Farmacotécnica – Natal, RN. Corresponding author: [email protected] Introduction: Helicobacter pylori is a bacteria associated with diseases such as peptic ulcer and gastric cancer. Amoxicillin and metronidazole are antimicrobials of choice to treat H. pylori infections. The current treatment, however, is made difficult mostly because of the insufficient gastric-residence time of these drugs, in a way that effective concentrations are not reached. Polymeric microparticulated systems are advantageous because they are able to deliver drugs in a proper site of absorption and in an appropriate amount. The use of natural polymers such as chitosan is of great interest, because of its biocompatibility, degradability, non-toxicity and mucoadhesion. Objetive: The aim of this study is to incorporate amoxicillin and metronidazole in chitosan microparticles to be delivered to the gastric mucosa, and also to evaluate drug entrapment efficiency and granulometry. Methods: Simple coacervation method was used to prepare the particles. Samples were prepared in different drug-polymer ratios (1:1 and 1:2) and in different concentrations (0,25 and 0,5%). Drug loading studies were carried out by UV/Vis spectrophotometry and size distribution was done by optical microscopy (FERRET). Results: The samples showed irregular surfaces and homogeneity in size distribution (sizes varying from 5 to 20 µm between all samples). Results for drug entrapment efficiency showed that about 23% of amoxicillin was successfully loaded into the particles (1:1 0,5%). For the metronidazole-containing samples, the loading was about 16% (1:2 0,25%). Conclusions: Results showed that higher proportions of the polymer as well as higher concentrations led to better drug loading. Financial support: CNPq/Brazil.



161

CODE 154 VISCERAL LEISHMANIASIS: SURVEY OF NOTIFIED CASES OF

DISEASE IN THE CITY OF ASSU / RN Salgado, T. M. M.1,3; Silva, A. M. S.1,3; Oliveira, H. F. A.1,3; Dantas, M. G.1,3; Barbosa, V. S. A.2,3 1 Acadêmico, 2 Docente 3 Curso de Bacharelado em Farmácia, Unidade Acadêmica da Saúde, Centro de Educação e Saúde, Universidade Federal de Campina Grande (UFCG). Corresponding author: [email protected] Introduction: Visceral leishmaniasis (VL) is a serious chronic disease

potentially fatal to humans in which mortality may reach 10% when no proper treatment is instituted. In Brazil, the importance of LV occurs not only in its high incidence and wide distribution, but also the ability to take severe and lethal when combined with the picture of malnutrition and concurrent infections. Objective: This study aimed to make an

epidemiological survey of cases of visceral leishmaniasis in the period February 2007 to July 2011, the city of Assu, situated in the State of Rio Grande do Norte. Materials and Methods: We analyzed clinical and

epidemiological information recorded in databases of the Information System for Notifiable Diseases (SINAN), the Health Department of the Municipality and investigation forms for patients. Results and Discussion:

We confirmed by serology by ELISA and RIFI, 25 cases of the disease (71.42%) of 35 reported, equating to an average of 5.5 cases / year. Most affected were children (n = 15, 60%), predominantly male (n = 8; 53.33%). The drug of choice for treatment was the pentavalent antimony. All patients lived on the outskirts of the urban area. Between 2010 and 2011 samples were collected from 260 dogs and 109 of these were positive (41.9%). Were removed 83 dogs with positive serology and 72 without confirmation. Conclusions: The combined data suggest better attention to the control of

disease, environmental sanitation, vector control, the reservoir hosts and treatment of those infected.




162

CODE 156 OZAWA CALCULATION APPLIED TO THE KINETIC STUDY OF

DECOMPOSITION OF FLUCONAZOLE BY THERMOGRAVIMETRY Azevedo, W. M. de1; Almeida, V. D. de1; Silva, E. P. da1; Souza, F. C. de1; Aragão, C. F. S.2; Gomes, A. P. B.2

1Undergraduate, Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Professor, LCQMed, DFAR, UFRN, Brazil. Corresponding author: [email protected] Introduction: Several studies have shown that thermogravimetry is a rapid and convenient technique for the determination kinetic study of decomposition reactions. Arrhenius equation and model Ozawa have been used to calculate energy of activation and order of reaction from thermogravimetric curves. Objectives: In this study, fluconazole raw material and capsules were analysed by Ozawa model for dynamic TG curves. Materials and Methods: Fluconazole raw material obtained from Galena Química e Farmacêutica Ltda was used in this study. Dynamic thermogravimetric curves of fluconazole raw material were obtained using a Shimadzu model DTG-60 thermobalance at 10, 20, 40, 60 and 80°C.min–

1 heating rates up to 900°C (n=3) in a nitrogen (flow rate: 50 mL.min–1). It was weighted 7.0±0.5 mg in alumina crucible. The curves TG were analyzed using TASYS program from Shimadzu, TGA Kinetic Analysis Program (Ozawa Model). The following kinetic parameters by Ozawa model kinetic energy, order and frequency factor. Discussion and Results: The fluconazole showed the following kinetic parameters: order(0.0), kinetic energy (84.88 KJ/mol) and frequency factor(1.78 x 107 min-1) fo α – 0.1, as the fluconazole capsules presented the following kinetic parameters: order(0.1), kinetic energy (94.40 KJ/mol) and frequency factor(3.16 x 109 min-1) fo α – 0.1. Fluconazole raw material has greater thermal stability than the fluconazole capsules due has greater activation energy. This shows that the excipients of the formulation may interfere with the stability. Conclusions: Ozawa model can be used to determine the parameters kinetics and stability of raw material and formulations. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN



163

CODE 157.1 THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE014 BY

TG/DTA AND DSC Silva, E. P.1; Galvão, F. P.2; de Araújo Júnior, J. X.4; França, P. H. B.4; S n ’An , A. E. G.4; Aragão, C. F. S.3; Gomes, A. P. B.3

1Undergraduate, Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Posgraduate, LCQMed, DFAR, UFRN. 3 Professor, LCQMed, DFAR, UFRN, Brazil. 4 Professor, LPqRN/UFAL,ESENFAR, UFAL, Brazil. Introduction: Thermal analytical methods are precise and accurate techniques with low sample requirements, and can provide detailed information about new chemical entities even at the very earliest stages of discovery and development of the new compositions and drugs. Objective: In this work, the alkaloid synthetic WE014 was investigated by means of differential scanning calorimetry (DSC) and simultaneous thermogravimetry/differential thermal analysis (TG/DTA). The results allowed us to acquire information concerning these compounds in the solid state, including their thermal stability and thermal decomposition. Materials and Methods: The alkaloid WE014 was analyzed by TG/DTA on a SHIMADZU thermobalance, model DTG-60, using an alumina crucible, at 10 ºC/ min–1 heating rate up to 900ºC, under an atmosphere of nitrogen at 50 mL/ min–1. It was weighted 7.0±0.5 mg. The DSC curves were obtained for SHIMADZU calorimeter, model DSC-60, using an aluminum crucible, at 10ºC/min-1 heating rate up 450ºC. The curves were analyzed using TASYS program from Shimadzu. Results and Discussion: The alkaloid WE014 showed four steps of decomposition. The first step corresponds the weight loss the humidy, with 6.35%. The second step corresponds the decomposition and onset temperature was 215.82, with weight loss about 15.83%. The percentage of ash corresponds to 3.26% at 900 ° C.The DSC data showed a melting peak at 210.64 ºC, with onset at 207.69 ºC, Heat at 175.47 J/g and purity 97.22%. Conclusions: The TG/DTA and DSC can be used in the determination of the parameters stability and purity for pharmaceuticals. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN.



164

CODE 157.2 DETERMINATION OF KINETIC PARAMETERS BY OZAWA OF

ALKALOID SYNTETIC WE014 Galvão, F. P.2; Silva, E. P.1; Júnior, J. X. de Araújo4; França, P. H. B.4; S n ’An , A. E. G.4; Aragão, C. F. S.3; Gomes, A. P. B.3

1Undergraduate, Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Posgraduate, LCQMed, DFAR, UFRN. 3 Professor, LCQMed, DFAR, UFRN, Brazil. 4 Professor, LPqRN/UFAL,ESENFAR, UFAL, Brazil. Introduction: Thermal analytical techniques can provide important information regarding storage and stability of pharmaceuticals. Also, kinetic parameters obtained from thermoanalytical data are highly useful for making predictions of performance parameters of drugs, for example, ‘‘she f fe”. The samples used in the analysis are the type guanilhidrazonas aromatic alkaloids that reports of several guanilhidrazonas studied as antihypertensives (Foye, 1990; Hoffman, 2003), antidiabetic (Larsen, 2001, Edmonton, 2000), antineoplastic (Andreani, 2000; Foye, 1990; Brzozowski, 2007). Objective: The aim of this study was to determinate by Ozawa model the kinetic parameters for WE014. Materials and Methods: The curves TG alkaloid WE014 were obtained in the thermobalance SHIMADZU, model DTG-60, using an alumina crucible, at 10, 20, 40, 60 and 80 ºC/ min–1 heating rate up to 900ºC, under an atmosphere of nitrogen at 50 mL/ min–1. It was weighted 7.0±0.5 mg. The curves TG were analyzed using TASYS program from Shimadzu, TGA Kinetic Analysis Program (Ozawa Model). Results and Discussion: The alkaloid WE014 was analysed by TGA kinetic analysis in the heating rate 10, 20, 40, 60 e 80 ºC min-1.The following kinetic parameters were found: kinetic energy (110.51 KJ/mo ), SD (4.17), CV(3.77%) fo α – 0.1, in the analysis range: 213.92-225.95ºC, in the calculation range: 91.11-86.30%. Conclusions: The thermal analysis obtain results quickly and realible from non-emprical data, meanly kinetic parameters. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN



165

CODE 158 DEVELOPMENT OF GOLD NANOPARTICLES CONTAINING

DOCETAXEL Oliveira, R.1,2*; Barratt, G.1; Moine, L.1; Santa Maria, L.C.2

1Université Paris Sud, UMR CNRS 8612, Faculté de Pharmacie, Châtenay-Malabry, France. 2 Universidade do Estado do Rio de Janeiro, Instituto de Química, Brazil. Corresponding author: [email protected] Introduction: Prostate cancer is the third most important cancer in terms of incidence and the sixth in terms of mortality for men throughout the world. At the moment, it is treated with hormone therapy but this is only effective for 24-36 months before the patients develop resistance. One of the few active cytotoxic molecules is docetaxel (DOC) or Taxotere. The encapsulation of this molecule into organo-metallic nanoparticles which could target the cancer cells directly would be an original therapeutic approach. Objective: The aims of this project are to formulate gold nanoparticles carrying DOC, PEG and folic acid are a targeting ligand for PSMA and to evaluate them in vitro and in vivo. Materials and Methods: The gold nanoparticles were made by the Institut des Sciences Moléculaires in Bordeaux. Nanoparticle characterization Measurement of size and zeta potential by quasi-elastic light scattering Measurement of size and morphology by TEM Evaluation of the ability of the folic acid on the particle surface to bind to PMSA using the « Biacore » technique available at Châtenay-Malabry. Evaluation in vitro: The MTT was used to measure cell viability. Results: Particles size (nm) Zeta Potencial (mV)

nanoAu1 nanoAu2 nanoAu2 filtered*

nanoAu3 nanoAu3 filtered*

296,0 -22,2

121,8 -15,9

84,7 - 12,5

28,7 -6,1

25,0 -7,4

* The suspension containing the nanoparticles was filtered with a sterile syringe filter 0.2 mM VWR cellulose acetate. Conclusions: The results obtained so far indicate that gold nanoparticles are promising as drug carriers for prostate cancer treatment. Financial support from CAPES Brazil



166

CODE 160 EVALUATION IN VITRO OF SCHINUS TEREBINTHIFOLIUS

ANTIOXIDANT ACTIVITY BY CAPTURE OF FREE RADICAL 2,2-DIFENIL-1-PICRILIDRAZIL (DPPH) Silva, J. B. R.1; Amorim, A. F. C. 1;Caldas, G. F. R.1; Nóbrega, R. F.1; Silva, J. L. 1; Wanderley, A. G. W. 1,2; Lafayette, S. S. L.1,2

1Departament of Pharmaceutical Sciences, Federal University of Pernambuco (UFPE). 2 Department of Physiology and Pharmacology, Federal University of Pernambuco (UFPE). Introduction: Schinus terebinthifolius Raddi (Anacardiaceae) is used in traditional Brazilian medicine due its properties adstringent, antidiarrheal, diuretic, antibacterial, antipyretic, anti-inflammatory and antioxidant. Many of these properties are associated to the presence of polyphenols. Objective: Evaluate antioxidant activity in vitro of hydroalcoholic extract of stem bark of Schinus terebinthifolius (ST) by capture of free radical 2,2-difenil-1-picrilidrazil (DPPH). Materials and Methods: The antioxidant activity was determined by the hydrogen-donating ability of ST. Aliquots of 1 mL ethanolic solution of DPPH (30 mmol/L) was mixed with 2.5 mL of different concentrations of ST (2.5-250 μg/mL). The absorbance was measured after 10 min at 517 nm (Brand-Williams et al., 1995). The IC50 (concentration of sample required to inhibit 50% of the radical DPPH) was calculated. Data were express as mean±s.e.m. Differences between samples were analyzed by ANOVA nd Tukey’s es (p < 0.05). The synthetic antioxidant butilaldehyde hydroxytoluene (BHT) was used as positive control. Results and discussion: Significant DPPH radical scavenging activity was evident in all concentrations tested. ST was able to reduce the stable free radical DPPH with an IC50 8.5 μg/mL. The BHT, in similar conditions showed IC50 73.8 μg/mL. Oxidative stress due to an increase in free radical generation or an impaired endogenous antioxidant mechanism is an important factor that has been implicated in various diseases. Conclusions: Schinus terebinthifolius showed significant activity in the capture of DPPH radical, showing a high antioxidant activity, probably related to its content of flavonoids. The clinical relevance of these findings in requires further studies. Financial Support from FACEPE/Brazil.



167

CODE 161.1 ANTIMICROBIAL ACTIVITY OF Annona coriacea L. AGAINST ORAL

CAVITY BACTERIA Silva, K. M. A.1; Alencar, L. C. B.1; Clementino, E. L. C.1; Santana, C. P.1; Fernandes, F. H. A1; Brito, E. M. C.1,2; Medeiros, A. C. D.1,2*

1 Laboratório de Desenvolvimentos e Ensaios de Medicamentos, Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande – PB. 2 Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual da Paraíba, Campina Grande – PB. Corresponding author: [email protected] Introduction: Annona coriacea L. is a plant popularly used in the treatment of inflammatory processes, its leaves, seeds and roots are used for this purpose. Although known its effect during inflammatory processes, there are few studies of other medicinal properties of this plant. Objective: To investigate the antimicrobial properties of this plant on cariogenic and oral biofilm-forming bacteria. Methods: The hydroalcoholic extract of the A. coriacea bark was tested by the broth microdilution method, against the strains of oral biofilm: Streptococcus mutans (ATCC 25175), S. oralis (ATCC 10557), S. salivarius (ATCC 7073) and Enterococcus faecalis (ATCC 29212). The tests were performed in triplicate. Results and discussion: The screening carried out showed that the concentration of 100% of the A. coriaecea L. extract was able to inhibit the growth of S. oralis, S. salivarus and E. faecalis with the same concentration, which showed bactericidal effect. The organism S. mutans, in turn, showed a greater sensitivity to the extract, which was inhibitory at the concentration of 25% and bactericidal at the concentration of 50%. Conclusion: A. coriacea L. may interest to dentistry, since, in addition to its known anti-inflammatory properties, also proved its antimicrobial activity. Thus, other tests would allow the use of this plant in the treatment of oral cavity injuries, optimizing its effectiveness and ensuring the safety of treatment. Financial support from FAPESQ/MCT/CNPQ and PROPESQ/UEPB



168

CODE 161.2 MINIMUM INHIBITORY CONCENTRATION OF Acanthospermum

hispidum DC FRONT OF Streptococcus oralis BYTHE MICRODILUTION AND AGAR DIFFUSION METHODS Silva, K. M. A.1; Santos, R. L.1,2; Chaves, T. P.1; Santana, C. P.1; Fernandes, F. H. A.1; Brito, E. M. C.1,2; Medeiros, A. C. D.1,2*

1 Laboratório de Desenvolvimentos e Ensaios de Medicamentos, Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande – PB. 2 Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual da Paraíba, Campina Grande – PB. Corresponding author: [email protected]

Introduction: Acanthospermum hispidum DC popu y known s “Esp nho de C g no” s w de y used n northeastern Brazil, in the treatment of asthma, bronchitis, as antipyretic, expectorant, vermifuge and against intestinal aches.Objective: To determine the minimum inhibitory concentration (MIC) of Acanthospermum hispidum DC extract by agar diffusion method using the technique of the cylinder and microdilution in broth front of Streptococcus oralis. Materials and Methods: Extracts were obtained by maceration process followed by rotative evaporation. From these extracts we obtained ten dilutions of the starting concentration of 1mg/ml, diluted in DMSO (10%). These were tested against Streptococcus oralis (ATCC 10557) by the agar diffusion method and dilution in microplates. Results and Discussion: The extract of Acanthospermum hispidum DC showed no antimicrobial activity in any of the concentrations on Streptococcus oralis by microdilution method, however, by the agar diffusion method it was active with inhibition halos above 10 mm in all dilutions tested, since the concentration of 1 mg / ml to 0,05 mg/ml. Conclusions: The extract of Acanthospermum hispidum DC, when tested against strains of Streptococcus oralis, showed inhibition of bacterial growth only by agar diffusion method. Therefore, this method obeys Fick's Law, and proved to be more efficient. Financial support from FAPESQ/MCT/CNPQ and PROPESQ/UEPB.



169

CODE 162.1 ANTIMICROBIAL ACTIVITY OF Acanthospermum hispidum DC. BY

MICRODILUTION Alencar, L.C.B.1; Chaves, T. P.1; Nóbrega, F. P.1; Brandão, D. O.1; Barbosa, A. S.1; Araújo, R. M.1; Medeiros, A. C. D.1,2*

1 Laboratório de Desenvolvimentos e Ensaios de Medicamentos, Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande – PB. 2 Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual da Paraíba, Campina Grande – PB. Corresponding author: [email protected] Introduction: Acanthospermum hispidum is a widely used plant in traditional medicine around the world to combat various types of infections. Since there are differences in relation to parts of the plant to be used, it is necessary to investigate if there are differences in the antimicrobial potential of these parts. Objective: To evaluate and compare the antimicrobial activity of root extract of A. hispidum, in relation to the entire plant extract. Methods: The hydroalcoholic extracts (70%) of the root and the whole A. hispidum, obtained by maceration, were tested by broth microdilution method, in front of microbial strains: Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853) and Klesbisiela pneumoniae (ATCC 4352). Three repetitions were performed. Results and discussion: The screening carried out showed that both extracts were effective against S. aureus, P. aeruginosa and E. coli. The root extract showed bactericidal effect against P. aeruginosa and inhibitory against E. coli in a concentration less than the entire plant extract, while the inhibitory concentrations and bactericidal against S. aureus were the same in both extracts. There was no activity of the extracts against K. pneumoniae. Conclusion: Considering the results obtained, we can affirm that the root extract of A. hispidum was more effective against the strains tested. It is recommended to carry out further tests with this plant, to ensure its safe use and most effective way possible. Financial support from FAPESQ/MCT/CNPQ and PROPESQ/UEPB



170

CODE 162.2 MICROBIOLOGICAL COMPARISON OF Hyptis mutabilis Briq ACTION,

BY THE MICRODILUTION AND AGAR DIFFUSION METHODS Alencar, L. C. B.1; Chaves, T. P.1; Fernandes, F. H. A.1; Araújo, R. M.1; Santos, R. L.1,2; Barbosa, A. S.1; Medeiros, A. C. D.1,2*

1Laboratório de Desenvolvimentos e Ensaios de Medicamentos, Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande – PB. 2Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual da Paraíba, Campina Grande – PB. Corresponding author: [email protected] Introduction: Hyptis mutabilis Briq belongs to Lamiaceae family, is common y known s “A f v c -de-C boc o” nd popu y used s n -inflammatory, antiseptic, antipyretic, antispasmodic and antiflatulent. Objective: To compare the antimicrobial action of H. mutabilis Briq by the method of agar diffusion and microdilution in broth. Materials and Methods: The hydroalcoholic extract (70%) of the whole plant was obtained by maceration. This extract was tested against the strains Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853) e Klesbisiela pneumoniae (ATCC 4352), by agar diffusion (technique of cylinder) and microdilution in broth. Results and Discussion: The hydroalcoholic extract of H. mutabilis showed no microbiological activity against any of the strains tested by agar diffusion, but was active against all strains by the method of dilution in microplates, with a minimum inhibitory concentration (MIC) of 6.25% and a minimum bactericidal concentration (MBC) of 12.50% to S. aureus, 50.00 % and 100.00% of MIC and MBC respectively for Klesbisiela pneumoniae and Pseudomonas aeruginosa. To Escherichia coli MIC was 25.00 % and MBC 50.00%. Conclusions: Although the agar diffusion method, using the technique of the cylinder, is considered more analytical, this method did not show satisfactory results against the strains mentioned, while the microdilution tests showed a higher sensitivity. Financial support from FAPESQ/MCT/CNPQ and PROPESQ/UEPB



171

CODE 163.1 DIAGNOSIS OF HYGIENIC-SANITARY CONDITIONS AND

COMPLIANCE WITH GOOD MANUFACTURING PRACTICES IN FOOD SERVICE AT PUBLIC DAY CARE CENTERS IN CAMPINA GRANDE-PB Ramos Júnior, F. J. L.1; Ribeiro, J. L.2; Brandão, D. O.3 1 Discente de graduação, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 2 Discente de graduação, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 3 Discente de graduação, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB).

Corresponding author: [email protected] Introduction: Food borne Diseases (FBD) are clinical events resulting from ingestion of foods that may be contaminated, being the main sites of outbreaks of FBD the day care centers and the schools. Objective: Diagnose the sanitary-hygienic conditions in the services of food of day care centers and public schools of Campina Grande-PB. Materials and Methods: The study was carried out in a sample of some day care centers and schools. It was elaborate a check-list based on the Resolution RDC/ANVISA N. 275 and RDC/ANVISA N. 216. Besides, it was analyzed the microbial contamination of the water used in the preparation of foods, using the technique of the multiple tubes. The determination of mesophilic aerobic bacteria, recommended by Ordinance No. 518, of the Ministry of Health, was carried out according to standard methods by APHA (1955). Results and Discussion: Only 16,66 % of the institutions attended more than 75 % of the evaluated items being classified like Group 1; other 50 % obtained service between 51-75 % being classified like Group 2 and 33,33 % attended less than 50 % fits into Group 3. It was noticed that the number of samples in accordance with the microbiological criteria was higher in the institutions that had better service to the checklist of checking of the conditions sanitary-hygienic. Conclusion: The institutions reached a low level of service regarding the Good Practices of Manufacture, according to directives of the legislation, which demonstrates unsatisfactory sanitary-hygienic conditions for food production in these institutions.. Financial support from UEPB/Brazil.



172

CODE 163.2 PREVALENCE OF INTESTINAL PARASITES AMONG STUDENTS OF

PUBLIC DAY CARE CENTER IN THE CITY OF CAMPINA GRANDE-PB Ramos Júnior, F. J. L.1; Rodrigues, T. P.2; Medeiros, J. S.3

1 Discente de graduação, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 2 Discente de graduação, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 3 Professor Doutor, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). Corresponding author: [email protected] Introduction: Intestinal parasitic diseases are caused by helminths and protozoa that attack the intestinal tract of living things and, of the child, interfere with the proper development. Objectives: Identify intestinal parasites in children from a public day care center in the city of Campina Grande-PB e verify the presence of parasitic helminth structures in soil samples of the day care center. Materials and Methods: The study was descriptive and experimental, conducted through the application form and laboratory analisys on a sample of 79 students. The examinations were performed at the Laboratory of Parasitology of State University of Paraíba, using direct methods, of Hoffman, Pons and Janer, Rugai, Mattos e Brisola and the method of Graham. The soil was analyzed according to the method of Willis, adapted for research into soil. The survey of nematode larvae was performed using the technique of Rugai, Mattos and Brisola, with the adjustment proposed by Carvalho et al. The data were submitted to the Epi Info version 3.4. Results: Of the samples analyzed, 62% had some type of parasite, where the male gender the highest positivity (65%). The most prevalent species were Giardia lamblia, Blastocystis hominis, Ascaris lumbricoides, Entamoeba histolytica/Entamoeba dispar, Entamoeba coli and Trichuris trichiura. The soil samples had larvae that were classified as larvae of nematodes. Conclusion: Due to the epidemiological and strategic importance of this research, we suggest a larger number of such studies, aimed to standardize methodologies for gathering and analysis, targeting other areas at risk of environmental contamination. Financial support from UEPB/Brazil.



173

CODE 164 FORMULATION AND EVALUATION OF SUNSCREEN EMULSIONS

USING EXPERIMENTAL DESIGN Neves, J. K. O. 2; Apolinário, A. C.1; Silva, D. T. C.1; Soares, B. S. A.1; Santos, D. P. G.1; Véras, G.3; Silva, J. A.1,2

1 Departamento de Farmácia /Universidade Estadual da Paraíba/UEPB, Campina Grande, PB. 2 Programa de Pós-Graduação em Ciências Farmacêuticas/Universidade Estadual da Paraíba/UEPB, Campina Grande, PB. 3 Programa de Pós-Graduação em Ciências Agrárias/Universidade Estadual da Paraíba/UEPB, Campina Grande,PB. Corresponding author: [email protected] Introduction: Exposure to sunlight provides beneficial and essential effects, but excessive exposure to ultraviolet radiation can cause skin photo aging, erythema and different skin cancer. However, those effects can be prevented by the use of sunscreen emulsions. Objective: The aim of this work was to apply factorial design methods to reduce the number and time of the various steps required to formulate and evaluate stable sunscreen emulsions. Materials and Methods: Eight formulations were prepared according to a 23 factorial design employing the following factors and levels: Emulsifying agent type (Polawax® and Cosmowax®), emulsifying agent concentration (10 and 12 %) and emollient type (isopropyl myristate and decyl oloeate). The emulsion stability was estimated by the average size of the droplets, the emulsion spreadability and the emulsion conductivity and pH. The emulsions were prepared by the phase inversion technique. Results and Discussion: The average size of the droplets was influenced by factors emulsifying agent concentration and emollient type. According to the fact that minimum droplet diameter corresponds to the most stable emulsion, the increase in the amount of emulsifying agent and the use of emollient decyl oleate resulted smaller droplets. The emulsion conductivity was influencied by emulsifying agent type, emulsions made with Polawax® showed a higher value. In case of the emulsion spreadability and pH, the factorial design cannot be used for pharmacotechnique optimization of formulations because the values found were not altered by the factorial matrix. Conclusion: Experimental design allowed to optimize important parameters involved in the formulation of sunscreen emulsions.



174

CODE 165.1 INTRINSIC DISSOLUTION: THE INFLUENCE OF THE TECHNIQUE ON

THE KINETIC PARAMETERS EVALUATION OF ANTIRETROVIRAL EFAVIRENZ Moura, M. L. C1; Vilarinho, A. C. S. G.2; Nunes, L. C. C. 2; Alves, L. D. S.1; Soares, M. F. R.1,2; Sobrinho, J. L. S.1; Rolim-Neto, P. J.1 1Federal University of Pernambuco. Recife, PE, Brazil. 2 Federal University of Piaui. Terezina, PI, Brazil. Corresponding author: [email protected] Introdution: The intrinsic dissolution rate (IDR) is defined as the dissolution rate of pure substances under the condition of constant surface area. This dissolution can be done by two ways: rotating and stationary disk. Objective: This research aimed evaluates the difference between the two forms of intrinsic dissolution in the evaluation of the kinetic parameters of the antiretroviral drug efavirenz (EFV). Materials and Methods: Weighed 20mg of EFV, which was inserted in the cavity of the die (intrinsic dissolution apparatus) to be compressed for a hydraulic press. The intrinsic dissolution were performed in different times and constant pressure. All used the medium sodium lauryl sulfate (SLS) 0.5% and speed of 50 rpm. Results and Discussion: From the results obtained it was noted that the drug had a better dissolution profile in the method with stationary disk. We can correlate the results with the possibility of air bubbles formation and the differences on the hydrodynamic. Conclusion: Based on the above can be said that the intrinsic dissolution by stationary disk measure more accurately the drug dissolution of the EFV.



175

CODE 165.2 INTRINSIC versus APPARENT DISSOLUTION: INFLUENCE OF

COMPRESSION ON THE KINETIC EVALUATION OF ANTIRETROVIRAL EFAVIRENZ Moura, M. L. C.1; Vilarinho, A. C. S. G.2; Nunes, L. C. C. 2; Alves, L. D. S.1;

Soares, M. F. R.1,2; Sobrinho, J. L. S.1; Rolim-Neto, P. J.1 1 Federal University of Pernambuco. Recife - Brazil. 2 Federal University of Piaui. Terezina - Brazil. Corresponding author: [email protected]

Introduction The dissolution test is a in vitro predictive method to measure the drug availability in vivo. Objective To compare the effectiveness between the apparent and intrinsic dissolution test using the antiretroviral drug efavirenz (EFZ). Materials and Methods The EFV apparent dissolution was realized with apparatus 2 (paddle) and 10 mg EFV capsules. The intrinsic dissolution was realized with 20 mg EFZ tablets by a hydraulic press, in a stationary die. Before and after of compression was done x-ray diffraction (RXD) to evaluate the integrity of the EFV crystal form, drug that has polymorphism, and with bioavailability variant due to this. Both dissolution were done in the following conditions: 37°C, 50 rpm, medium with sodium lauryl sulfate 0.5%, sextuplicate and collect time of 15 and 60 minutes. Results and Discussion The results obtained showed that the intrinsic dissolution evaluated the drug performance more accurately when compared to the apparent dissolution (Qap=60.21% and Qint=14.68%, 60 min), however from the XDR can be seen that the compression interfered in the crystallinity of the drug, which could generate polymorphic forms of pressure, which affects the outcome in vitro and, consequently, in vivo of the EFV.



176

CODE 167 INVESTIGATION OF THE SPASMOLYTIC ACTION OF Solanum

stramonifolium Jacq. ON RAT AORTA AND GUINEA-PIG ILEUM Silva, M. C. C.1; Pereira, J. C.1; Correia, A. C. C.2; Martins, I. R. R.2; Souza, I. L. L.1; Oliveira, G. A.2; Travassos, R. A.2; Silva, T. M. S.3; Agra, M. F.4; Silva, B. A.4

1 L bo ó o de Tecno og F m cêu c “P of. De by Fe n ndes de Mede os”, Un ve s d de Fede d P íb , João Pesso /PB, B azil. 2 Programa de Pós-graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, João Pessoa/PB, Brazil. 3 Departamento de Química, Universidade Federal Rural de Pernambuco, Recife/PE, Brazil. 4 Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa/PB, Brazil. Introduction: Solanum genus (Solanaceae) is constituted of several species, including Solanum stramonifolium Jacq. which was chosen based on chemotaxonomic criterion since other species already showed spasmolytic action. Objective: perform a comparative study of crude ethanolic extract of green (SS-EtOHFV) and ripe (SS-EtOHFM) fruits of S. stramonifolium for a possible spasmolytic activity on rat aorta and guinea-pig ileum. Methodology: organs were suspended in organ baths in appropriate conditions. Isotonic and isometric contractions were monitored and recorded. All the experimental protocols were approved by Ethical Committee in Animal Research of LTF/UFPB (Protocol 0506/05). Results: SS-EtOHFV and SS-EtOHFM (243 and 729 µg/mL) showed no significant spasmolytic activity on rat aorta pre-contracted by phenylephrine in both absence and presence of functional endothelium. On guinea-pig ileum, SS-EtOHFV and SS-EtOHFM (243 and 729 µg/mL) inhibited significantly the phasic contractions induced by histamine (Emax = 64.3 ± 1.0 and 61.1 ± 4.7%, respectively). Interestingly, when the agonist used was carbachol, a different effect was observed: whereas SS-EtOHFV (243 and 729 µg/mL) inhibited significantly the phasic contractions (Emax = 67.2 ± 10.5%), in the other hand SS-EtOHFM inhibited the phasic contractions in a significant and concentration dependent manner (IC50 = 217.5 ± 31.6 μg/mL). Conclusions: Both extracts have not vasorelaxant effect in rat aorta. However, SS-EtOHFM showed a better spasmolytic activity than SS-EtOHFV on guinea-pig ileum. Thus, it is suggested that the fruit ripeness degree affected the pharmacological effect of the tested extracts, possibly due to qualitative and quantitative changes of S. stramonifolium secondary metabolites. Financial Support from CNPq/CAPES.



177

CODE 168 KINETIC STUDIES FOR ITRACONAZOLE USING OZAWA MODEL BY

DYNAMIC THERMOGRAVIMETRY Almeida, V. D. de1; Azevedo, W. M. de1; Silva, E. P. da1; Souza, F. C. de1; Aragão, C. F. S.2; Gomes, A. P. B.2 1Undergraduate, Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Professor, LCQMed, DFAR, UFRN, Brazil. Corresponding author: [email protected] Introduction: Thermogravimetry (TG) is a thermoanalytical technique used in pharmaceutical studies for the characterization, purity, compatibility of formulations, the identification of polymorphism, stability assessment. In previous studies to characterize the kinetics of thermal decomposition were used the Ozawa model for curves non-isothermal and Arrhenius equation for isothermals. Objective: Evaluate application of the Ozawa model for characterization of thermal decomposition kinetic of Itraconazole at dynamic TG curves. Materials and Methods: The Itraconazole raw material coated pellets analyzed was obtained from Farmafórmula Manipulation Pharmacy. For kinetic determination, non-isothermal TG curves were obtained using a Shimadzu thermobalance, model DTG–60. Alumina crucible with 10.5 mg (±0.5) of sample under a nitrogen flow at 50 mL.min–1 and heating rates of 10, 20, 40, 60 and 80°C.min–1 up to 900°C (n=3) was used for the process. The kinetic parameters were determined via Ozawa model and Arrhenius equation. Discussion and Results: The itraconazole showed the following kinetic parameters: order(0.3), kinetic energy (99.09 KJ/mol), SD (1,27), CV(1,35%) and frequency factor(1.02 x 1010 min-1) fo α – 0.1, as the itraconazole capsules presented the following kinetic parameters: order(0.3), kinetic energy (95.56 KJ/mol), SD (2,08), CV(2,18%) and frequency factor(1.02 x 1010 min-1) fo α – 0.1. Itraconazole raw material has greater thermal stability than the itraconazole capsules due has greater activation energy. This may indicate interactions with excipients in the formulation since they should improve stability. Conclusions: Through the application of the Ozawa model can determine the stability of pharmaceuticals and raw materials. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN



178

CODE 169 PHYTOCHEMICAL STUDY AND ANTI-INFLAMMATORY ACTIVITY OF

AQUEUS EXTRACTS AND FRACIONS OF MIMOSA TENUIFLORA (JUREMA PRETA) AND HANCORNIA SPECIOSA (MANGABEIRA) Bitencourt, M. A. O.1; Lima, M. C.1; Torres, M.1; Furtado, A.1; Langassner, S. M. Z.2; Fernandes-Pedrosa, M. F.1*

1 Laboratório de Tecnologia e Biotecnologia Farmacêutica, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 2 Laboratório de Farmacognosia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] INTRODUCTION: The species Mimosa tenuiflora and Hancornia speciosa, are plants used in traditional medicine for various diseases. Due to lack of scientific studies, it is necessary to conduct studies to characterize the active compounds of the species. OBJECTIVE: Within this context, the objective of this work was the preliminary analysis of the majority compounds and anti-inflammatory activity of aqueous extracts and fractions of H. speciosa and M. tenuiflora. The decoction was subjected to a liquid-liquid partition with solvents of increasing polarity: dichloromethane (3x300mL), ethyl acetate (3x300mL) and n-butanol (3x300mL). MATERIALS AND METHODS: The phytochemical fractions were performed by Thin-Layer Chromatography (TLC). To evaluate the anti-inflammatory activity, we essayed the model of against carrageenin-induced paw edema and peritonitis. RESULTS AND DISCUSSION: Analysis by TLC was observed flavonoid fractions of ethyl acetate and n-butanol in H. speciosa, and dichloromethane and n-butanol in M. tenuiflora. In the paw edema model were tested three doses (4, 20 and 40mg/kg), revealing a significant inhibition of edema in the fourth hour. For the bioassay-guided fractionation is used the model of peritonitis where he e w s s gn f c n educ on eukocy e’s m g on n n m s e ed w h the aqueous extracts at all three doses tested (20, 30 and 40mg/kg), and fractions dichloromethane, and ethyl acetate. CONCLUSION: In parallel, the bioassay-guided fractionation is being developing to characterize the active compounds of the aqueous extract of H. speciosa and M. tenuiflora. Financial Support from CNPq and CAPES.



179

CODE 170 THERMAL ANALYSIS MULTCOMPONET COMPLEX WITH Β-CD AND

METHOTREXATE Barbosa, J. A. A.1; Oliveira, A. R.1; Melo, P. N.1; Rocha, H. C. S.2; Oliveira, A. G.3; Silva-Júnior, A. A.1

1 Programa de Pós-Graduação em Ciências Farmacêuticas. Universidade Federal do Rio Grande do Norte, UFRN, Natal- RN. 2 Curso de Farmácia, UFRN, Natal- RN. 3 Faculdade de Ciências Farmacêuticas de Araraquara, UNESP, Araraquara-SP. Introduction: Methotrexate (MTX) is a drug widely used in psoriasis, but its systemic use may cause several side effects. An available alternative is a topical drug delivery, which lead to increase the drug permeation and its bioavailability in the region of damaged skin. However, topical formulation requires good drug solubility.Thus, cyclodextrins are excipients that can form soluble complexes and in association with hydrophilic polymers or hydrophilic substances may present a synergistic effect in the icrement of drug solubility. Objective: This study aimed to characterize the inclusion complexes with MTX obtained by freeze-drying. Materials and Methods: The TG curves were obtained using thermal balance in the range 0 to 450 °C with a heating rate of 10 °C min-1, submitted under an atmosphere of N2 (50 ml.min-1) with aluminum pan. The samples were pure β-CD, pure MTX, physical mixture the compounds and the ternary complexes containing polymers PEG 10000, Polaxamer 407, PVA, PVP and the hydrophilic compound TEA. Results and Discussion: Acco d ng o TG of β-CD occur a first event at about 61- 103ºC with mass loss 10%, corresponding to evaporation of adsorbed water and crystallization, and second event took place at about 314-350 °C with mass loss 66% , representing of decomposition temperature. The curve of MTX the first event occurred at about 75-117 °C with mass loss 4%, characteristic of phase transition of MTX due to loss of water of crystallization of its structure, and a second event at 236-286 °C with mass loss of 31%, characteristic of decomposition of the drug. To suggest the formation of inclusion complexes using a TG its necessary to compare the temperature of the beginning of degradation of the drug alone and with the complex, and complexes degradation occurs in higher temperatures. The SEM images showed wo c ys ypes fo MTX nd egu fo m o β-CD. The inclusion complexes presented different forms, suggesting the obtention of inclusion complexes. The XRD results demonstrated the different pattern of the pure compounds suggesting the obtaining of binary and ternary complexes with drug in amorphous phase. Conclusion: The events can suggest complex form, however, it is important to complement various other techniques that confirm the formation of complexes. Financial support: CAPES and CNPq



180

CODE 171 PSEUDO-TERNARY PHASE DIAGRAMS AS A TOOL TO PRODUCE

EMULSION E MICROEMULSION Silva, D.T.C.1; Apolinario, A.C.1; Souza, M.S.R.1; Fernandes, J.J.G.1, Damasceno, B.P.G. L.2; Silva, J.A.2 1Universidade Estadual da Paraíba (UEPB), Laboratório de Desenvolvimento e Caracterização de Produtos Farmacêuticos (LDCPF), 58429-600, Campina Grande-PB-Brazil. 2UEPB, Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), LDCPF, 58429-600, Campina Grande-PB-Brazil, Corresponding author: [email protected] Introduction: The pseudo-ternary phase diagram construction (PTPD) is an essential step to emulsions (E) and microemulsions (ME) production. The PTPD describes the experimental condition in which it is possible to obtain microemulsions and transition boundary regions between emulsions, phases separated and microemulsions O/ A and A / O. ME is isotropic, transparent and thermodynamically stable systems. They are usually produced with 4 ingredients: water, oil, surfactant and co-surfactant, in the PTPD, so the aqueous phase, oil phase and mixture of surfactant / co-surfactant are represented at the vertices of the triangle. This type of diagram also supports other mixtures, such as oil, co-surfactant and water /surfactant. Objectives: Apply the PTPD as a tool to obtain stable E and ME. Materials and Methods: The PTPD were produced by the mixture of Plurol Oleique® (CS) and Labrasol® (S) [2:8; 4:6; and 6:4, respectively], with the Captex® 355 [oil phase (O)] in the range from 1:9 to 9:1. To this S/CS/O mixture, the aqueous phase (water Milli-Q) was titrate with an automatic pipette, and shaken with magnetic stirrer. Results and Discussion: Two regions of cosmetic and pharmaceutical interest were found: ME and semisolid E. The optically transparent liquid systems were considered ME, on the other hand, the viscous and opaque systems were called semisolid E. Conclusion: These results allow us to conclude that PTPD is a good, simple and reliable technique to characterize the area of E and ME regions.



181

CODE 172 DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF

HYDROALCOHOLIC EXTRACT PLANT ON CANDIDA SPECIES OF MEDICAL INTEREST Batista, R. S. A.1, Silva, G. S.2, Machado, S. E. F.², Feitosa, I. L. F. F.², Vieira, K. V. M.3, Dantas, I. C.4, Felismino, D. C.5

¹ Laboratório de Microbiologia, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB); ² Laboratório de Microbiologia, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB); ³ Departamento de Farmácia, Laboratório de Microbiologia, Universidade Estadual da Paraíba (UEPB); 4 Departamento de Farmácia, Laboratório de Botânica, Universidade Estadual da Paraíba (UEPB); 5 Departamento de Biologia, Laboratório de Botânica, Universidade Estadual da Paraíba (UEPB). Corresponding author: e-mail: [email protected] Introduction: The use of medicinal plants has been widely seen as a normative basis for the maintenance of health. Objective: To determine the minimum inhibitory concentration (MIC) of hydroalcoholic extracts of Lippia sidoides Cham, Punica granatum L. and Syzigium aromaticum L. front of the Candida species related to vulvovaginal candidiasis (VVC) and oral candidiasis (OC), Candida albicans (ATCC 18804), C. parapsilosis (ATCC 22019), C. krusei (ATCC 34135), C. tropicalis (ATCC 13803), C. guilhermondii (ATCC 6260). Materials and Methods: First, the strains were grown; then, they were suspended in saline on the standard scale of 0.5 Mac Farland. The MIC was determined by agar diffusion technique, cavity method. From the crude extract (100%), there were serially diluted in sterile saline to 50%, 25%, 12.5%, 6.25%. In each hole, it was deposited 50μL of e ch d u on. They we e ncub ed 37˚C/24-48h. The extracts were considered active when showed growth inhibition halos with diameters bigger than 10mm. Results and Discussion: Of the extracts tested, dilutions of the extract of Lippia sidoides Cham showed the inhibition against strains of C. krusei and C. tropicalis, determining the 25% dilution and the MIC (11mm halo), and the extract of Syzigium aromaticum L. which showed inhibition against the strain of C. guilhermondii, dilution 50% (10.5 mm halo). Conclusion: It was observed that the plant species Lippia sidoides Cham showed antifungal activity, prompting further research on it, using preliminary data in determining the future of herbal medicine against Candida species causing VVC and OC. Financial support from PIBIC/UEPB



182

CODE 174 THE CURINE ANTIINFLAMMATORY EFFECT IS A CYTOKINE

DEPENDENT MECHANISM. Carvalho, F. L.¹ (PG); Alves, A. F¹*.; Ferreira, L. K. D. P.¹; Costa, H. F.¹, Piuvezam, M. R.¹ (PQ). ¹Departamento de Fisiologia e Patologia, Centro de Ciências da Saúde. UFPB/PB Corresponding author: *[email protected] Introduction: Curine is a bisbenzylisoquinoline alkaloid from Chondrodendron platyphyllum (Menispermaceae) and inhibited cell migration to inflammatory site. Objective: The goal of this study was to demonstrate the antiinflammatory mechanism of curine. Material and Methods: Swiss mice (n=6-7) were treated with saline, curine (2.5 mg/kg) or dexamethasone (2.0 mg/kg) and one hour later was administered carrageenan or bradykinin in the left paw and the edema was measured by paquimeter. The vascular leakage was evaluated by injection (i.p.) of acetic acid solution into the peritoneum and amount of protein was measured by spectrophotometer. The inflammatory cell migration and cytokine/chemokine production (TNF-α, IL-1β, IL-6, CXCL-1 and MCP-1) was analyzed into the pleural fluid of animals previously challenge with LPS by citometric assay. Statistical analyses were performed by one-way ANOVA. Results and Discussion: The curine treatment inhibited edema formation induced by carrageenan but did not by bradikinin. Also curine inhibited (p<0.05) vascular leakage and LPS-induced polymorphonuclear migration (P<0.05) as compared with the control group. In addition the secretion of TNF-α, IL-6 and MCP-1 but not IL-1 was inhibited (P<0.05) by curine. Dexamethasone reduced edema formation as well as inhibited the cytokine production as expected. Therefore the present study demonstrated that curine treatment regulates inflammation by inhibiting cytokine and chemokine secretion as well as neutrophil migration to the site of inflammation. Conclusion: The data suggest that curine is a potent antiinflammatory drug by inhibiting the cell migration through a mechanism dependent of MCP-1, TNF-α and IL-6 production. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil



183

CODE 175.1 DTA/TGA/DTG ANALYSIS OF Euterpe oleracea (AÇAÍ) GLYCOLIC

EXTRACT Rodrigues, R. O. [1]; Daher, C. C. [2]; Fontes, I. S. [1]; Soares, D. S. [1]; Silva, E.P. [3]; Gomes, A. P. B. [3]; Ferrari, M.[2]; Aragão, C. F. S. [3] 1 Laboratory of Research and Development of Cosmetic Products - Faculty of Pharmacy, Federal University of Rio Grande do Norte. 2 Faculty of Pharmacy - Potiguar University 3 Laboratory of Quality Control – Faculty of Pharmacy, Federal University of Rio Grande do Norte. Corresponding author: [email protected] Introduction: Euterpe oleracea is a specimen of Amazon composed of polyphenols whose antioxidant activity is used in cosmetics preparations with benefits for skin. Thermal methods and equipment related have been improved in the past decade and logically their application is currently much wider than previously. The thermals methods are tecnics used in cosmetics for to charaterize actives, pre-formulation studies, thermal stability studies and purity. Objectives: The aim of this work was to evaluate the thermal behavior of açaí glycolic extract. Materials and Methods: The açaí glycolic extract was analyzed by TG/DTA. The TG curves were obtained with a Shimadzu thermobalance (DTG-60), under a N2 flow 50 mL.min-1, at heating rate 10oC.min-1, up to temperature of 900ºC. Sample mass about 8mg were packed in an alumina cell. TG/DTA curves was analyzed with the aid of SHIMADZU software TASYS. Results and Discussion: TGA curve reveled two stages in range of 45 - 73°C (-35%) and second at 119 - 138°C (-62%). With 3% of the residue. DTG data showed -0,85 mg and -3.34 mg. DTA curve show two endothermic event (degradations) happens to acaí glycolic extract at temperatures (Tonset, peak, Tendset) in (33, 71, 99°C) with an energy of -3.90 kJ/g, and second at (117, 135, 149°C) and of -1.65 kJ/g energy. Conclusions: A thermal analysis study on decomposition processes of açaí glycolic extract show your thermal instability and these parameters can be used in the development and characterization of cosmetics products. Financial support from CNPq/Brazil and scholarship from UFRN/Brazil.



184

CODE 175.2 KINETICS STUDIES OF UVB SUNSCREENS: ETHYLHEXYL TRIAZONE

AND ETHYLHEXYL METHOXYCINNAMATE Rodrigues, R. O. [1]; Daher, C. C. [2]; Fontes, I. S. [1]; Soares, D. S. [1]; Aragão, C. F. S. [3]; Gomes, A. P. B. [3]; Ferrari, M.[2] 1 Laboratory of Research and Development of Cosmetic Products - Faculty of Pharmacy, Federal University of Rio Grande do Norte. 2 Faculty of Pharmacy - Potiguar University. 3 Laboratory of Quality Control – Faculty of Pharmacy, Federal University of Rio Grande do Norte. Corresponding author: [email protected] Introduction: Ultraviolet (UV) radiation in sunlight is divided in UVC, UVB and UVA. This investigation focused on ethylhexyl triazone and ethylhexyl methoxycinnamate used for protection against UVB radiation. Objectives: The aim this work was avaliable and compares the kinetics behavior of sunscreens. Materials and Methods: The TG curves were obtained with a Shimadzu thermobalance (DTG-60), under a N2 flow 50mL.min-1, at heating rate 10oC.min-1, up to temperature of 900ºC. Sample mass about 10mg were packed in an alumina cell. For the decomposition, the activation energy (Ea), the order of reaction (n) and the frequency factor (Z) were obtained from first step of TG curves. The curves obtained were analyzed for software Shimadzu TA60 TASYS. The kinetic parameters

were calculated with values of 0.1–0.9 by utilizing the mathematical equations of Coats-Redfern (CR), Madhusudanan (MD), Horowitz-Metzger (HM), and Van-Krevelen (VK). The kinetic data were calculated by four mathematics models. Results and Discussion: Presents the values of the order of reaction (n), the Ea and the Z for ethylhexyl triazone and ethylhexyl methoxycinnamate, with the best linear correlation coefficients (r): ethylhexyl triazone (n / Ea - kJ.mol-1 / Z/s-1): CR (0.55/211.42/6.75x1013); MD (0.66/220.57/3.90x1014); HM (0.69/242.02/1.93x1016); VK (0.64/241.31/7.24x1021); ethylhexyl methoxycinnamate – CR (0.31/91.46/3.80x106); MD (0.07/82.71/4.66x105); HM (0.29/107.56/1.36x108); VK (0.29/100,55/9.28x1012). TGA data allowed calculating kinetic parameters. The Ea by used methods have shown good results correlation. Conclusions: Thermal data of the sunscreens supply important information on evaluation of stability of UVB protection, becoming an important tool on quality control and development of cosmetics products. Financial support from CNPq/Brazil and undergraduate scholarship from UFRN/Brazil.



185

CODE 176 LECTIN ANTINOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY

EXTRACTION, PURIFICATION AND EVALUATION OF Canna limbata ROSCOE SEEDS. Tenório, A. P. M.2; Araújo, T. S.1; Falcão, M. A. P.2; Maleski, A. L. A.2; Melo, S. M. S.2; Da Silva, F. C.2; Silva, D. F.1; Correia, I. A. L.1; Machado, S. S.1; Martins, J. L.3; Cavada, B. S.4; Da Rocha, B. A. M4; Moreira, M. S. A.5; Mella, E. A. C.5

1 Instituto de Química e Biotecnologia, Universidade Federal de Alagoas (UFAL); 2 Universidade Federal de Alagoas (UFAL); 3 Instituto de Química e Geociências, Universidade Federal de Pelotas; 4 Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará (UFC); 5 Instituto de Ciências da Saúde, Universidade Federal de Alagoas (UFAL) Corresponding author: [email protected] Introduction: Lectins are proteins that are capable of specific recognition of carbohydrate and because of that have several actions, like in immunology, cell recognition, histology, pharmacology and others. Objective: Extract e purify lectin of the Canna limbata seed, followed by the evaluation of the antinociceptive and antiinflammatory activity. Materials and Methods: Proteics extracts were obtained by seeds milling e through saline extraction followed by precipitation with ammonium sulfate, as methodology adaptation described by OITA et al, 2000. Affinity chromatology using chitin was used in isolation and purification of lectin molecules. The purification accompaniment was made by hemagglutinating activity and electrophoresis techniques (sds-page). The experiments were made using swiss mice (20-30g) approved by Etic Commyte – UFAL (nº: 015102/2010-51). The nociception and inflamation experiments were: abdominal writhing, nociception e peritonitis, induced by, respectively, acetic acid, formalin and carrageenan. The results were analyzed by t test in prisma®(*p<0.05,*<0.01) tutorial. Results and Discussion: The e’s hint of a tetrameric protein (~50kda) consisting of subunit of about 12kda. In writhing experiment, lectin presented significant inhibitory efect (10mg/kg=76%, 5mg/kg=70% and 1mg/kg=53%). In formalin experiment, the lower lectin concentration has the best nociceptive answer in fase 1 (1mg/kg=50%, 5mg/kg=41% and 10mg/kg=30%), and in fase 2 (1mg/kg=75%, 5mg/kg=60% and 10mg/kg=63%) resembling indomethacin. The peritonitis experiment was able to inhibit in a static way the cell migration. Conclusion: The Canna limbata seeds lectin structural aspects resembling with the others recognizers lectins of monocotiledone purified mannose, besides the properties antiinflammatory and peripheral antinociceptive activity was observed. Financial Support from CNP and FAPEAL




186

CODE 177.1 RHEOLOGICAL ANALYSIS IN PRELIMINARY STABILITY STUDIES OF

SUNSCREEN EMULSIONS CONTAINING AÇAI EXTRACT (Euterpe oleraceae). Fontes, I. S. 1; Daher, C. C.2; Rodrigues, R. O. 1; Soares, D. S. 1; Ferrari, M.1 ¹Laboratory of Research and Development of Cosmetics Product, Department of Pharmacy, Federal University of Rio Grande do Norte. ² Faculty of Pharmacy - University Potiguar. Corresponding author: [email protected] Introduction: The rheological properties study can be used to select different formulations in the development of cosmetic emulsions. Rheological measurements of the formulations play an important role in the physical stability. Objective: The aim of this work was to evaluate the influence of the both emulsifying agent and viscosity modifiers on the rheological properties in the development of cosmetic emulsions. Materials and Methods: Emulsions were prepared with different concentrations of Acrylates/C10-30 Alkyl Acrylates Crosspolymer (0.1% - P1, 0.2% - P2, 0.3% - P3). The stable emulsion was added of Carbomer (0.1% - PC1, 0.2% - PC2, 0.3% - PC3). The organoleptic characteristics, centrifugation test, freezing-thawing cycle (FTC) and thermal stress (TS) were used to investigative the preliminary stability tests. The physical preliminary stability was assessed through rheological determinations. The minimal apparent viscosities and flow index values were evaluated. Results and Discussion: The P1 and P2 formulations were unstable in the centrifugation test. The addition of rheological modifier improved preliminary stability. The PC2 and PC3, formulations have been found to be preliminary stables. The PC1 formulation showed statistical differences after TS and FTC tests. The viscosity modifier concentration used influenced the rheological behavior of the formulations. The rheological measurements showed that the samples exhibited non-Newtonian pseudoplastic flow behavior. Conclusions: The P3, PC2 and PC3, formulations were stables in the standard conditions and should be conducted to accelerated stability tests. The authors thank Basf, Croda, ISP and Mapric for the gift of sample materials. Financial support from CNPq/Brazil and undergraduate scholarship from UFRN/Brazil.



187

CODE 177.2 EVALUATION OF SUNSCREENS THERMAL ANALYSIS BY

THERMOANALYTIC TECHNIQUES Fontes, I. S. 1; Daher, C. C. 2; Rodrigues, R. O. 1; Soares, D. S. 1; Aragão, C.F.S. 3; Gomes, A.P.B. 3; Ferrari, M.1

¹ Laboratory of Research and Development of Cosmetic Products, Faculty of Pharmacy, Federal University of Rio Grande do Norte (UFRN). ² Faculty of Pharmacy - Potiguar University ³ Laboratory of Quality Control, Faculty of Pharmacy, Federal University of Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Exposure to solar radiation has a variety of harmful effects on human health. Some of these effects include the induction of photosensitization, immunosuppression, photocarcinogenesis and photoaging. However, the ultraviolet (UV) dose absorbed by the skin can be reduced by limiting exposure to the sun through the use of photoprotective agents such as sunscreens. Consequently a high chemical stability is an important requirement for an effective UV filter. The thermal analysis techniques have been used for the characterization of raw materials and formulation studies. Objective: The aim of this work was to evaluate the sunscreens thermal stability by thermoanalytic techniques: thermogravimetry (TG) and differential thermal analysis (DTA). Materials and Methods: The TG curves were obtained with a Shimadzu thermobalance, model DTG-60, under a N2 flow 50 mL.min-1, at heating rate 10 oC.min-1, up to temperature of 900 ºC. Sample mass about 10 mg were packed in an alumina cell. The curves obtained were analyzed for software Shimadzu TA60 TASYS. Results and Discussion: The following results in descending order of the sunscreens studied and their heats of reactions by DTA and onset temperature by TG: Bis-ethylhexyloxyphenol methoxyphenyl triazine (-12, 02 KJ/g; 431,55ºC); Ethylhexyl triazone (-12,89 KJ/g; 397,2ºC); Diethylamino hydroxybenzoyl hexyl benzoate (-19,94 KJ/g; 309ºC). Conclusions: According to the analyses we found that Bis-ethylhexyloxyphenol methoxyphenyl triazine decomposed at temperatures above and is more stable that the other sunscreens. Financial support from CNPq/Brazil and undergraduate scholarship from UFRN/Brazil.



188

CODE 179 STUDY OF ASSOCIATION BETWEEN OPG POLYMORPHISMS

(- 163A>G; – 1181G>C) AND mRNA EXPRESSION IN TYPE 1 DIABETES PATIENTS Loureiro,M.B.1; Ururahy,M.A.G.1; Oliveira, Y.M.C. 1; Souza, K.S.C.1; FREIRE NETO, F. P.1; BEZERRA, J. F. 1; SILVA, H. P. V. 1; OLIVEIRA, G.H.M 1; Hirata,R.D.C.2; Arrais,R.F.3; Almeida,M.G.1; Hirata,M.H.2; Rezende,A.A.1

1 Department of Clinical and Toxicological Analyses-UFRN/Natal-RN/Brazil; 2 Department of Clinical and Toxicological Analyses-USP/São Paulo-SP/Brazil; 3 Department of Pediatrics-UFRN/Natal-RN/Brazil Introduction: Several studies suggest that children and adolescents with type 1 DM present the risk in long term to suffer a reduction in bone mass, which could limit the bone growth and increase the probability of developing osteoporosis and its complications. Objectives: The objective of present study was to investigate the association between OPG (- 163A>G; – 1181G>C) polymorphisms and mRNA expression of OPG in type 1 diabetic children and adolescents assisted at a pediatric hospital (HOSPED/UFRN) in Natal-RN/Brazil. Materials and Methods: This study included 100 type 1 diabetic patients (DM1 group) and 106 normoglycemic subjects (NG group) aged between 6 and 20 years. Metabolic control was evaluated by glucose and glycated hemoglobin. Expression of mRNA was performed by PCR real-time. Polymorphism was determined by allelic discrimination technique in real-time PCR using TaqMan® pre-designed SNP assays from Applied Biosystems. Results and Discussion: Glucose and glycated hemoglobin were significantly increased in diabetic individuals compared to NG, indicating a poor metabolic control of these patients. OPG polymorphism (- 163A>G) (NG: TT-71%, CT-28% and CC-1%; DM1: TT-73%; CT-26% and CC-1,56%) was associated to increased OPG mRNA expression in type 1 diabetics. No association was found between OPG polymorphism (– 1181G>C) (NG: CC-38%, CG-47% and GG-15%; DM1:CC-36%; CG-47% and GG-17%) and OPG mRNA expression. Conclusions: The increased OPG mRNA expression in the presence of the OPG (- 163A>G) polymorphism could reflect a possible protection against bone resorption associated with type 1 diabetes. Finnantial Support from CNPq, CAPES.

http://lattes.cnpq.br/7464620984028550



189

CODE 180 THIRD ORDER EXPERIMENTAL APPROACH FOR HUMIDITY DETERMINATION IN

THE LEAVES OF Azadirachta indica A. Juss

Emerenciano, D. P.1*

, Rodrigues, C. J. F.1, Oliveira, W. A.

2, Maciel M. A. M.

1, Moura M. F.

V.1, LIMA, K. M. G.

1.

1Institute of Chemistry, Center of Exact Sciences and Earth - CCET, Federal University of

Rio Grande do Norte, UFRN, Natal / RN, Brazil. 2Department of Pharmacy, Health Sciences Center - CCS, Federal University of Rio Grande

do Norte, UFRN, Natal / RN, Brazil. Corresponding author: *[email protected]

Introduction: The medicinal species Azadirachta indica A. Juss (Meliaceae) is widely cultivated in Asia, Africa and other tropical parts of the world. Scientific studies developed throughout the world, led to the isolation and structural elucidation of more than 100 special metabolites, among them terpenoid-types. In this work a third order experimental approach was used to leaves obtained from A. indica, aiming both of them punctual and effectiveness results to determine its humidity. The applied experimental method revealed a succsseful and practical methodology that could be utilized to any plant species work aiming subsequent analysis, such as bromatologicals ones. Objective: Development of a third order experimental approach to determine humidity in the leaves of Azadirachta indica A. Juss for subsequent bromatologicals analysis. Material and Methods: The leaves of Azadirachta indica A. Juss were daily collected (in May 2011) during four days on the UFRN/Natal Campus University. The third order experimental approach was realized according to ranging parameters such as: vegetal weight (5 and 10 g), temperature (95 and 105°C) and drying period (10 and 15 min.). For that, the reference method Analytical Standards of the Institute Adolfo Lutz (1985) was applied. In this method, the humidity of the tested vegetal sample (10 g utilized in triplicate) was dried at 105°C for 24 h. After this period, heating and cooling (at room temperature in desiccator) were performed at intervals of 1 h, followed by weighing (until constant weight measurement). In this, present work the adapted analysis were carried out with plant mass, temperature and drying period variants on OHAUS-MB200 balance apparatus coupled with infrared. Results and discussion: Determination humidity analysis following the reference methodology (Normas Analíticas do Instituto Adolfo Lutz, 1985) afforded 68.03% (+/- 0.02) data. The obtained results by the third order experimental approach realized according to specifically ranging parameters, monitored via statistical analysis, afforded variance average around 3.33 with a Tcritical (Tc) value of 5.43%, which was adopted as the reference data for the investigation of those used parameters). According to the obtained results it was proved that the best conditions for the modified method involves the following parameters: lower plant mass (5 g), drying period of 15 min. at the higher temperature (105ºC). For the whole daily analysis it was possible to have significant drying results ranging from 63.29% to 66.72%. Specifying, 66.72% (+/- 1.53) for the first day vegetal collection, 63.29% (+/- 0.26) for the second one, 64.27% (+/- 0.36) and 66.39% (+/-1.51) for those ones (values are the average of daily triplicates). Conclusions: Based on the development of a third order experimental approach aiming to determine humidity in the leaves of Azadirachta indica A. Juss for subsequent bromatologicals analysis, it was proved that the reference result (68%) and the obtained data from the approached method (63.29% to 66.72% observed in four days experimental time) were similar. In that, it was concluded that the modified method showed significant efficacy with a drastic reduction of analysis time consumption, since the reference method involves analysis within 10 days time. Financial support from CNPq / Brazil and CAPES / Brazil References:



190

CODE 181 HUMAN LEUCOCYTE ANTIGEN REGION AND DIABETIC

NEPHROPATHY IN CHILDREN AND ADOLESCENTS WITH TYPE I DIABETES OF RIO GRANDE DO NORTE, BRAZIL Silva, H.P.V.1, Ururahy, M.A.G.1, Loureiro, M.B.1, Souza, K.S.C.1, Oliveira, Y.M.C.1,Freire-Neto, F.P.1,Bezerra, J.F.1, Oliveira, G.H.M.1,Hirata, R.D.C.2, de QuateliDoi, S.3, Donadi, E.A.4, Arrais, R.F.1, Almeida, M.G.1, Hirata, M.H.2, Rezende, A.A.1

1 Universidade Federal do Rio Grande do Norte (UFRN) - RN 2 Universidade Estadual de São Paulo (USP) – São Paulo - SP 3 Uniformed Services University of the Health Sciences(USU) -Maryland - USA 4 Universidade Estadual de São Paulo (USP) – Ribeirão Preto - SP Corresponding author: [email protected] Introducion. The human leucocyte antigen (HLA) region has a major influence on susceptibility to Type I diabetes (TDM1) and is possibly implicated in susceptibility to diabetic complications as the diabetic nephropathy. Objective. The purpose of this study was to examine the role of human leucocyte antigen genes (HLA-DRB1,-DQA1, and -DQB1) in susceptibility to Type 1 diabetes and diabetic nephropathy. Materials and Methods. The study was conducted on 29 children and adolescents with type 1 diabetes under treatment with insulin assisted at the pediatric hospital (HOSPED/UFRN) in Natal/RN, Brazil, and 71 healthy volunteers representing control group (CG), with mean 6 to 20 years old in the groups. DNA was extracted from the peripheral blood mononuclear leukocytes of patients and controls by use of the Qiagen Kit, Quiagen. The HLA-DRB1 and DQB1 gene alleles were examined by the polymerase chain reaction (PCR – SSO reverse). Results and discussion. The most significant alleles associated with T1DM were DRB1*03:01 (p>0001; OR=11,79), DQA1*05:01 (p>0001; OR=9,03) and DQB1*02:01 (p>0001; OR=4,81)). In addition, the genotypes DRB1*03:01-DRB1*04:05 (p=0,0061; OR=25,24), DQB1*02:01-DQB1*03:02, (p<0,0001; OR=88,89) and DQA1*03:01-DQA1*05:01 (p<0,0001; OR=47,67) were positively associated with T1DM. The haplotypes DRB1*03:01-DQA1*05:01-DQB1*02:01 (p<0,0001; OR=11,79) and DRB1*04:05-DQA1*03:02-DQB1*03:02 (p=0,0084; OR=13,30) had a stronger association with susceptibility to T1DM. 7 of 29 T1DM patients had microalbuminuria and presented the following alleles: DRB1*04:05 (p=0,0095; OR=0,0094) and DQA1*03:02 (p=ns) and genotypes: DRB1*03:01-DRB1*0405 (p=0,0419; OR=0,070) and DQA1*03:02-DQA1*05:01 (p=0,0419; OR=0,070) but no significant association with diabetic nephropathy was seen. Conclusions These results strengthen the effect of HLA-Class II on susceptibility to T1DM in children and adolescents of Rio Grande do Norte. No demonstrate the major role of HLA in the susceptibility to nephropathy.. Financial support from CNPq/Brazil.

http://www.usuhs.mil/



191

CODE 182 PREPARATION AND CHARACTERIZATION OF INCLUSION

COMPLEXES FORMED OF ESSENTIAL OILS OF Lippia sidoides Cham AND Lippia origanoides HBK WITH Β-CYCLODEXTRIN. Medeiros, M.G.F.1, Mourão, L.C.S.2, Silva, A.C.3, Figueiredo, R.C.B.Q.3 Moura, T.F.A.L.2, Lopes, J.A.D.4. 1 Department of Biochemistry and Pharmacology, Federal University of Piauí (UFPI) – Teresina – Brazil. 2 Laboratory of Drug Development, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN) – Natal – Brazil 3 Department of Microbiology, Research Center Aggeu Magalhães, FIOCRUZ-PE. – Recife – Brazil. 4 Department of Chemistry, Federal University of Piauí (UFPI) – Teresina – Brazil. Corresponding author: [email protected] Introduction: Lippia sidoides Cham and Lippia origanoides HBK species belong to the Verbenaceae family, being widely cultivated in Brazil. They present biological activities, such as antimicrobial and antiparasitic properties. The formation of inclusion complexes of CDs with essential oils increases the stability of the oil and reduces its volatilization which allows its handling and application. Objectives: The aim of this study was to prepare and characterize inclusion complexes of essential oils of Lippia sidoides and Lippia origanoides in β-cyclodextrin aiming their application in the treatment of leishmaniasis. Material and Methods: Inclusion complexes were obtained by the technique of kneading. About 2.0 g of

essential oil was added to 10 g of a moist paste of -cyclodextrin and a solution of ethanol-water (1:2). During 30 minutes, the mixture was mixed in a mortar until obtaining a solid product which was submitted to drying (70 ° C, 16 hours) and sprayed. The samples were characterized by DSC, SEM and H1 NMR and the profiles of the oils in natura, the complexed preparations and the patterns (carvacrol and thymol) were compared. Results and Discussion: The DSC curves showed disappearance of the characteristic peak of the essential oils in natura. SEM revealed changes in the characteristic p e n of β-CD in the presence of essential oils. The NMR presented chemical shifts to high field for the carbons 3 and 5 in the p esence of β-CD complexed with the standards and essential oils. Conclusion: The physical-chemical characterization of preparations suggests a probable formation of inclusion complexes between the essen o s nd β-CD. Master scholarship from CNPq – CAPES/Brazil.



192

CODE 183 ASSOCIATION OF POLYMORPHISMS IN THE IGF-1, TGF-Β1 AND

LRP5 GENES WITH TYPE 1 DIABETES Souza, K.S.C.1; Ururahy, M. A. G.1; Oliveira, Y. M. C. 1; Loureiro, M. B.1; Silva, H. P. V. 1; Freire-Neto, F. P.1; Bezerra, J. F. 1; Santos, F. M. 1; Morais, L. V. F.; Hirata, R. D. C.2; Arrais, R. F.3; Almeida, M. G.1; Hirata, M. H.2; Rezende, A.A.1

1 Department of Clinical and Toxicological Analyses-UFRN/Natal-RN/Brazil; ² Department of Clinical and Toxicological Analyses-USP/São Paulo-SP/Brazil; ³ Department of Pediatrics-UFRN/Natal-RN/Brazil Corresponding author: Rezende, A.A.¹: [email protected] Introduction: Type 1 diabetes (T1DM) is a multifactorial disease with an e y ge of onse , n wh ch he nsu n p oduc ng β ce of he p nc e s e destroyed because of autoimmunity. Polymorphisms in genes, like IGF-1, TGF-β1 nd LRP5, cou d be ssoc ed o ype 1 D be es (T1DM) nd s complications. Objectives: The objective of present study was to investigate the association between IGF-1 (-1245G>A), TGF-β1 (-509C>T) and LRP5 (126188 C>T) polymorphisms in the development T1DM in patients assisted at a pediatric hospital (HOSPED/UFRN) in Natal-RN/Brazil. Materials and Methods: This study included 134 type 1 diabetic patients (T1DM group) and 181 normoglycemic subjects (NG group) aged between 6 and 20 years. Metabolic control was evaluated by glucose and glycated hemoglobin. Polymorphism was determined by allelic discrimination technique in real-time PCR using TaqMan® pre-designed SNP assays from Applied Biosystems. Results and Discussion: Glucose and glycated hemoglobin were significantly increased in diabetic individuals compared to NG, indicating a poor metabolic control of these patients. LRP5 (126188 C>T) polymorphism was associated to T1DM (NG: CC-83.4%, CT-16% and TT-0.6%; T1DM: CC-70.7%, CT-27.1% and TT-2.2% - p=0.020). No association was found between IGF-1 (-1245G>A) (NG: GG-45.52%, GA-47.59% and AA-6,90%; T1DM:GG-53.66%; GA-39.02% and AA-7.32% - p=0.626) and TGF-β1 (-509C>T) (NG: GG-46.90%, GA-40% and AA-13.10%; T1DM:GG-41.46%; GA-45.12% and AA-13.41% - p=0.998) polymorphisms and T1DM. Conclusions: These results revealed an association between LRP5 (126188 C>T) polymorphism on susceptibility to T1DM in children and adolescents, showing their possible relationship in the pathophysiology of the disease. Financial Support from CNPq/Brazil.

http://lattes.cnpq.br/7464620984028550



193

CODE 184 VALIDATION OF UV – SPECTROPHOTOMETRIC ANALYTICAL

METHOD FOR THE DETERMINATION OF PRAZIQUANTEL IN INCLUSION COMPLEXES WITH Β-CYCLODEXTRIN IN PRESENCE OF HYDROXYPROPYLMETHYLCELLULOSE. Mourão, L.C.S.1, Batista, D.R.M.R.1, Morais, W.A.1, Moura, T.F.A.L.1

1 Laboratory of Drug Development, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN).

Corresponding author: [email protected] Introduction: Praziquantel (PZQ) is the drug of choice for the treatment of schistosomiasis, however, is classified as Class II in the Biopharmaceutics Classification System due to its low solubility. The use of cyclodextrins is a useful tool for increasing the solubility and bioavailability of class II drugs. Objective: The aim of this study was to develop and validate a quantitative UV spectrophotometric method for determination of praziquantel in inclusion complexes with β - cyclodextrin and hydroxypropylmethylcellulose. Material and Methods: The validation parameters were assessed according to The International Conference on Harmonization (ICH) and American Pharmacopoeia guidelines. To numerically evaluate the quality of fit of the models, we used analysis of variance (one-tailed test, p <0.05). The means were evaluated by Student's t test (two-tailed test, p <0.05). Results and Discussion: The linearity range was 120-1020 μg.mL-1, regression equation: absorbance = 0.00111 x PZQ concentration (μg.mL-1) + 0.00508 and r = 0.9998. For the other parameters were analyzed triplicate concentrations of 120, 570 and 1020 μg.mL-1. The repeatability of the method was 2.66, 1.41 and 0.56% and intermediate precision was obtained: 3.41, 1.70 and 0.30%. The method proved to be selective, robust against changes in temperature, the solvent and the wavelength used. The accuracy indicated that the difference among mean percentages of recovery were no statistically significant (p <0.05). The detection and quantification limits of praziquantel were found to be 0.22 and 0.667 μg.mL-1 respectively. Conclusion: The proposed method is accurate, precise and reproducible for estimation of praziquantel as raw material and in pharmaceuticals dosage forms such as inclusion complexes. Financial support from Master scholarship from CNPq – CAPES/Brazil.



194

CODE 185 EVALUATION OF COMPONENTS OF METABOLIC SYNDROME IN

ADOLESCENTS WHO ARE OVERWEIGHT ACCORDING TO THE NATIONAL CHOLESTEROL EDUCATION PROGRAM - ADULT TREATMENT PANEL III Melo, M.M.A.¹; Ramos, A.T.R.¹; Medeiros, C.C.M.²; Farias, A.A.L¹; Félix, B.F.¹ ¹ Department of Pharmacy, State University of Paraíba (UEPB) ² Center for Studies and Research and Epidemiology (NEPE), State University of Paraíba (UEPB) Corresponding author: [email protected] Introduction: The Metabolic Syndrome (MS) features by a set of metabolic disorders that if no treated and/or prevented can be initiate related diseases to cardiovascular risk (CVR). Objective: Evaluate the components of MS among overweight adolescents in order to raise awareness of the current world problems. Materials and Methods: Cross sectional study of 146 overweight adolescents conducted between April 2009 and March 2010 at the Center for Childhood Obesity, Campina Grande-PB. To obtain the MS we used NCEP-ATPIII values. Has been physical examination, biochemical evaluation and body composition. In the proportions associated analysis of the diagnostic criteria with the patient gender, we used the Qui-square, has been adopted a significance level less than or equal to 5%. Results and Discussion: 81.5% of the sample we e obese (pe cen e ≥95) nd 18.5% ove we gh (pe cen e 85 ≥ 95). The MS has been detected in 39 (26.7%) of patients, most of obese girls. Low HDL-c was the most common component, in 127(87%) of the patients. There wasn´t significant difference between the genders (p>0.05) for the values of triglycerides, glucose and blood pressure. However, the results of abdominal circumference and HDL-c showed discrepancies between the normal and abnormal proportion. For the first, 16% of boys and 55.2% of girls presented abnormal values (x2=20.77; p<0.001). For the HDL-c 92.7% of girls and 76% of boys(x2=8.10; p=0.004) presented changes. Conclusion: The obesity degree influenced in the appearance of changes that make up the MS, suggesting more predisposition of CVR in overweight adolescents.



195

CODE 186 ENERGETIC ASPECTS OF CHEMICAL INTERACTION BETWEEN THE

VIOMICYN ANTIBIOTIC AND BACTERIAL RIBOSOME Vianna, J. F¹; Fulco, U. L¹; Albuquerque, E. L¹; Oliveira, J. I. N¹; Neto, J. X. L¹ 1 Laboratory of Biophysics and Computational Simulation, Department of Biophysics and Pharmacology, at Federal University of Rio Grande do Norte, 59072-970, Natal, RN, Brazil. Corresponding author: [email protected] Introduction: The Mycobacterium tuberculosis is the causative agent of tuberculosis, a disease that provoked 2 million deaths in 2007, and estimates of 9 milion new recurrences. This bacterium has become resistant to antibiotics commonly used, and thus triggered a major public health problem. The Viomycin is a new anti-tuberculosis aminoglycoside that inhibits protein synthesis by preventing the translocation step in translation, due to stabilization of tRNA in site A. Objective: In this study, we measure the energy of interaction of this drug on bacterial ribosome in order to understand its pharmacological action. Materials and Methods: From the viomycin-70S ribosome crystal, the energy calculations were carried out by DFT-MFCC method. Calculations were made for each nucleotide present at the binding site, considering a radius of 20 Å from the center of mass of the ligand. Results and Discussion Thus, identify these C1491 (-12,954Kcal/mol), C518 (-9,762Kcal/mol), G1494 (-8,38Kcal/mol), A1408 (-8,085Kcal/mol) and C1409 (-4,886Kcal/mol) are residues that interact more strongly with the drug. The calculated interaction energies p ov de ns gh on spec fic d ug b nd ng nd e ve y usefu o ou understanding of drug binding to ribosomes. Financial support from CNPq/Brazil.



196

CODE 187 VALIDATION OF A HPLC METHOD FOR THE QUANTIFICATION OF

DIBUCAINE IN SOLUTION AND ENCAPSULATED IN SOLID LIPID NANOPARTICLES OR NANOSTRUCTURED LIPID CARRIERS Barbosa, R. M1, Marcato, P.2, Franz-Montan, M1, Bella, T.1, de Paula, E.1.

1 Department of Biochemistry, State University of Campinas (UNICAMP). C.P. 6109, CEP 13083–970, Campinas, SP, Brazil. ² Chemistry Institute, Biological Chemistry Laboratory, State University of Campinas (UNICAMP), C.P. 6154, Campinas, CEP 13083-970, S.P., Brazil. Corresponding author: [email protected] Introduction: Pain is the focus of many studies in the scientific community. Local anesthetics (LA) relieve pain by reversibly blocking the process of excitation-conduction in peripheral nerves. Dibucaine (DBC), used mainly in topical formulations, is one of the most potent long-acting local anesthetics, but is also one of the more toxic. Recently, Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) have attracted attention as a promising carrier for drug delivery. Objective: The purpose of the present study was to develop and to validate an analytical methodology for quantifying DBC into SLN and NLC formulations, prepared through high pressure with different lipid matrixes (myristyl myristate, MM or cetyl palmitate, CP) by HPLC. Materials and Methods: The quantification was done using the reversed-phase C18 column, a mobile phase consisting of a mixture of acetonitrile and triethylamine phosphate buffer (pH 2.3, 0.04M, v/v) (55:45 v/v), a flow rate of 1.0 mL/min and UV-Vis detector at 247 nm. Results and Discussion: The results show that

the analytical methodology is accurate, reproducible and robust; the method was linear in the concentration range 1.5–correlation coefficient (r =0.999). The limit of quantification and detection

dibucaine was over 78% for SLN and NLC samples. Conclusions: The

developed method was applied in the analysis of DBC encapsulated in SLN and NCL yielding good results. It will be used in future studies with different nanoparticle formulations. Financial support from Fapesp and Capes.



197

CODE 188.1 EVALUATION OF ANTIBACTERIAL POTENTIAL OF THE LEAVES OF

Senna occidentalis (L.) LINK Feitosa, I. L. F.¹; Machado, S. E. F.1; Batista, R. S. A1; Felismino, D. C.²; Dantas, I. C.³ ¹ Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). ² Departamento de Biologia, Universidade Estadual da Paraíba (UEPB). Corresponding author: [email protected] Introduction: The use of natural resources as an auxiliary mechanism in the treatment of different diseases has risen, with an emphasis in recent times. Objective: This study aimed investigate the antimicrobial activity of Senna occidentalis (L.) Link leaves in the form of fluid extract and lushness in the face of standardized strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Materials and Methods: The evaluation of the antimicrobial activities of extract and lushness were performed by disk diffusion technique on solid space. In the screening, the disks were sterile filter paper, which were impregnated with 20μL fluid extract or lushness at concentrations of 100%. As a positive control, antibiotics Gentamicin disks were used for the strain of S. aureus and Aztreonam disks for the other strains; as a negative control, paper disks of sterile filter, impregnated in water-alcohol solution at 70°GL were used. The assays were performed in triplicate and incubated at 37°C/48 hours. Halos of growth inhibition equal or bigger than 8 mm in diameter would prove the antibacterial effect of plant species. Results and Discussion: The results showed that the leaves of Senna occidentalis showed no antimicrobial activity against the strains tested, as opposed to literature. Conclusions: We suggest using the fractionated extraction and other methods of incubation in order to prove the effectiveness of this plant.



198

CODE 188.2 EVALUATE OF THE ANTIFUNGAL ACTIVITY OF Senna occidentalis

(L.) LINK.’S LEAVES FORWARD TO STRAINS OF Candida spp Feitosa, I. L. F.¹; Machado, S. E. F.1; Batista, R. S. A1; Felismino, D. C.²; Dantas, I. C.² ¹ Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). ² Departamento de Biologia, Universidade Estadual da Paraíba (UEPB) Corresponding author: [email protected] Introduction: Recently the resistance to antimicrobial drugs prove its lower efficiency, due to increased microbial resistance, overuse of these. The research of new substances were intensified because of this fact. The use of herbal extracts and forms of natural remedies has gained importance in current therapy. The Senna occidentalis (Mangerioba) has therapeutic effects as anti-inflammatory and antimicrobial. Objective: The s udy ev u ed he n fung of he Senn occ den s’s e ves n concentrated forms of fluid extract and lushness in the face of standardized strains of Candida tropicalis, C. guillermondi, C. krusei, C. parapsilosis and C. albicans. Materials and Methods: The antifungal activity of the extract and the lushness were evaluated separately by the disk diffusion technique on solid space. Paper discs impregnated were used with 20μL sterile filter and extract lushness. As a positive control, we used the Nystatin disks, and the negative control were made using discs with water-alcohol solution at 70°GL. The tests were made in triplicate and incubated at 37°C/48 hours. The halos of growth inhibition equal or bigger than 10 mm diameter confirming the effect of antifungal Mangerioba. Results and Discussion: There was no antimicrobial activity of this plant, being contrary to the literature. The solvent (ethanol 70ºL) may not have been ideal to extract the active ingredients in fungicidal effect against the strains. Conclusions: It need to repeat the study using new methods such as fractionated extraction and other alternative objective evidence of incubation activity.



199

CODE 189 CHITOSAN NANOPARTICLES FOR PROTEIN DELIVERY OBTAINED

BY IONIC GELATION Soares, K. S. R1; Fonseca, J. L. C2; Santos, K. S. R1; Silva-Júnior, A. A1; Fernandes-Pedrosa, M.F.1

¹ Graduate Program on Pharmaceutical Sciences, Federal University of Rio Grande do Norte, UFRN, Natal-RN, Brazil. ² Chemistry Departament, UFRN, Natal, RN. Introduction: Anti-sera against the scorpions are routinely produced by

various institutions and despite its effectiveness depend on the quality and speed with which treatment is started. Several studies have been developed in the research for appropriate technologies to encapsulate and release recombinant proteins capable of inducing antibody production. Much attention has been given to chitosan to be a bioactive natural, non-toxic, biocompatible and biodegradable. It has been extensively investigated for formulating a nanocarrier for therapeutic macrosolutes, particularly genes and protein molecules. Objective: This study aims to obtaining nanoparticles based on chitosan by ionic gelation, to delivery peptides / proteins of the venom of the scorpion T. serrulatus, able to provide a new system of immunization in animals, in order to obtain a new serum polyclonal anti T. serrulatus venom. Material and Methods: Chitosan nanoparticles were prepared by ionic gelation methodology using the crosslinker agent polyanion tripolyphosphate (TPP). The evaluation of the size distribution of particles was performed using the Zeta Sizer. Different parameters such as volume and concentration of reagents, pH and zeta potential of the solutions were evaluated with aim to optimize the methdodlogy. Results and Discussion: Chitosan nanoparticles were successfully obtained using established parameters for ionic gelation method. The best conditions optimized lead to obtaining nanoparticles with size about 187,13+-5,11. Conclusions: The methodology was optimized successfully. Chitosan nanoparticles prepared by ionic gelation technique presented a very suitable particle size distribution. A direct correlation among the parameters of the reagents concentration and zeta potential was identified cleary. Financial support from CNPq/Brazil



200

CODE 190 CLINICAL-EPIDEMIOLOGICAL PROFILE OF PATIENTS WITH BREAST

CANCER IN TO THE COMBINATION CANCER CHEMOTHERAPY 5-FLUOROURACIL, EPIRUBICIN AND CYCLOPHOSPHAMIDE Falcão, A.H.P.B.M.1, Cunha, G. H.,Leite, A.L.A.S. ,* Costa, M.A.R 2

,Jamacaru, F.V.F.3; Moraes,M.E.A.

¹ Unidade de Farmacologia Clínica, Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará (UFC) ² Acadêmica de Farmácia, Faculdade de Farmácia, Universidade Federal do Ceará (UFC) ³ Unidade de Farmacologia Clínica, Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará (UFC) Corresponding author: [email protected] Introduction: Breast cancer is one of the most frequent among women. Risk factors include personal and family history of breast cancer, early onset of menstruation and ending late, no pregnancies or pregnancies late, among others. Objective: To identify the profile of patients with breast cancer undergoing chemotherapy combination with 5-fluorouracil, epirubicin and cyclophosphamide (FEC) in a clinic in Teresina-PI. Materials and Methods: The research was through medical records. The project was submitted to the Ethics Committee of the Federal University of Ceará, and approved on 14/05/2009 under protocol No. 127/09 COMEP. Results and Discussion: Only 23 patients underwent chemotherapy with FEC regimen. The age ranged from 39 to 76 years (mean: 54.3 years, SD: 9.29). With regard to reproductive life, the average age of menarche was 13.7 years and 47.2 years of menopause. The average number of pregnancies and deliveries were 2.2 and 1.8, respectively. Regarding marital status, 12 patients were married and 11 unmarried (single, separated and widowed). Regarding lifestyle, 8.7% were smokers and consumed alcoholic beverages. The study population, 10 women (43.4%) reported family history of cancer. In addition, 21.7% said they breastfed and 13.0% had a history of oral contraceptive use as a con traceptive method. Conclusions: The study showed that the sample had some risk factors which increase the possibility of a woman developing breast cancer.



201

CODE 191.1 ATIMICROBIAL ACTIVITY OF BIOACTIVITY METABOLITES ISOLATED

FROM Streptomyces spp. FRONT OF PATOGENIC FUNGI YEAST OLIVEIRA, G. N.1; OLIVEIRA, T. L. 2; LIMA, E. O. 2; FREITAS, F. I. S.2

1 Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde (CCS), Universidade Federal da Paraíba (UFPB). 2 Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde (CCS), Universidade Federal da Paraíba (UFPB). Introduction: Due to the prevalence of fungal infections with strains multiresistant to antibiotics used in therapy, we seek to further treatment as an alternative to the bioactive metabolites from natural sources. Among the Actinobacteria is the genus Streptomyces are bacteria isolated from soils with high potential for production of antibiotics. Objective: The objective of the search for native strains of Streptomyces spp order to characterize the antimicrobial activity against pathogenic fungi from yeast extracts of Streptomyces spp. Materials and Methods: 03 strains were isolated from soil of the city of Joao Pessoa-Pb, obtained by suspension of the soil sample and streaked into a Petri dish. The identification was performed by macro-morphological, micromorphological, and physiological tests. The antimicrobial screening was determined by the agar block technique, and the dry ethanolic extract was prepared from the species with the best results in screening. The antagonism was determined using the disk diffusion testing on solid medium plates and microdilution Elisa. Results and Discussion: The inhibition halos obtained from the extract JPA and JPA-S1-S2 showed an antagonistic effect with values between 10-20 mm above the halos of inhibition promoted by the drug control. Conclusions: The results of the microdilution minimum inhibitory concentrations were significant between 10 mg and 5 mg. The results characterize an antimicrobial potential of the extract against the Candida spp species of clinical origin. It is appropriate and worthwhile to conduct further sampling of soil, revealing study of Streptomyces spp. biotechnology as an important source for obtaining new bioactive compounds. Financial support CNPq/Brazil.



202

CODE 191.2 ACUTE TOXICITY OF CARVACROL

Lima, I. O¹; Oliveira, G. N2; Nóbrega, F. M.2; Dias, G. E. N.3; Oliveira; Thompson L.4; Diniz, M. F. F. M.4. 1 Programa de pós-graduação em Produtos Naturais e Sintéticos Biativos / Centro de Ciências da Saúde (CCS) / Universidade Federal da Paraíba (UFPB) 2 Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde (CCS), Universidade Federal da Paraíba (UFPB). 3Programa de pós-graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos / UFPB/ Universidade Federal do Rio Grande do Norte (UFRN) / Universidade Federal do Ceará (UFC) / Universidade Federal Rural de Pernambuco (UFRPE) 4 Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde (CCS), Universidade Federal da Paraíba (UFPB). Introduction: Carvacrol is a monoterpene with antibacterial, antifungal, antiinflamantory, antioxidant actions. The acute toxicity of mix of carvacrol (93%) and thymol (7%) was studied in mice at 225mg/kg (v.o.), but there is not registers about its effect on biochemical and hematological paramenters (Fitoterapia, v. 75, p.801-3, 2004). Objective: The aim of this work was to evaluate the toxicity of carvacrol (98%) in mice with a simple dose. Material and methods: Mice of either sex (six females and males, weight of 25-35g) received 250 mg/kg of carvacrol (v.o.) and another group received the vehicle (control). The animals were observed during 14 days and the last day, the mice were euthanized. The biochemical and hematological paramenters was evaluated, the weight of organs and any alterations were observed (Psicofarmacologia, p.131-7, 2006; RE 90/2004). Results and Discussion: The carvacrol caused the lost of corneal reflex at 30 minutes after its administration. This phytoconstituent decreased the water consumption in male and female mice. The monoterpene increased the aspartate amino transferase (AST) in male mice. There were not died mice. Conclusions: These results showed the low toxicity of carvacrol, however it is necessary more studies with phytoconstituent. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil



203

CODE 192 EVALUATION OF THE TOXICITY OF Pradosia huberi

HYDROALCOHOLIC EXTRACT ON THE HYPOPHYSIS-GONADAL AXIS OF PREGNANT FEMALE RATS Rocha, A. O. B1; Souza, L. Q. S.1; Sousa, T. M1; Figueiredo, R. R.1; Potiguara, R. K. S.1; Pita, J. C. R1; Santos, E. C. S.1; Medeiros, A. A. N.2;Diniz, M. F. F. M.3; Sá, R. C. S.4

1. Program of Post graduation in Natural and Syntactic Bioactive Products, Center of Health Sciences – LTF/UFPB, Brazil 2 .Institute of Technological Research of Amapá - IEPA, Brazil 3. Department of Pharmaceutical Sciences, Center of Health Sciences - UFPB, Brazil 4. Department of Physiology and Pathology, Center of Health Sciences - UFPB, Brazil Corresponding author: [email protected] Introduction: Pradosia huberi Ducke (Sapotaceae) is a medicinal plant commonly found in the Amazonian forest and popularly used to treat gastritis and gastric ulcer. The presence of saponins, flavonoids, steroids and triterpenes has been identified in the stem bark of this species. Objectives: In this study, the toxicological effect of the hydroalcoholic extract (HAE) obtained from P. huberi barks was investigated on the organs of the reproductive axis (hypophysis, ovaries and uterus) of pregnant female rats. Materials and Methods: Female Wistar rats were distributed into four groups (containing 15 or 16 animals each), in which three of them were treated orally with HAE of P. huberi (T1 = 1,22; T2 = 6,1; T3 = 30,5 mg/kg) and one received distilled water, once daily, from the first to the seventh day of gestation (preimplantation phase). The animals were killed by an overdose of ketamine on the 8th day of gestation. After laparatomy, the following organs were removed and weighed (absolute and relative weight): hypophysis, ovaries and uterus. Results and Discussion: The relative weight of the hypophysis of the treated groups (T1 = 0,049 ± 0,002*; T2 = 0,054 ± 0,003* and T3 = 0,051 ± 0,003*) increased significantly, when compared to the group control (C =0,040 ± 0,001) on the 8th day on gestation. Conclusions: The results indicate a possible toxic effect of P. huberi HAE on the hypophysis, which could interfere with the normal functioning of the hypophysis-gonadal axis. Financial support from CAPES/Brazil.



204

CODE 193.2 DRUG PROFILE OF ELDERLY DIABETES AND DYSLIPIDEMIA

ATTENDED BY PHARMACEUTICAL CARE PROGRAM IN A COMMUNITY PHARMACY. Brandão, D. O. 1; Palmeira, A. C.2, Fernandes, F. H. A. 1; Clementino, E. L. C. ; Simões, M. O. S. 1,2,3, Medeiros, A. C. D. 1,3*

1Departamento de Farmácia, Universidade Estadual da Paraíba , Campina Grande – PB, Brasil, 2Programa de Pós-graduação em Saúde Pública, Universidade Estadual da Paraíba, Campina Grande – PB 3Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual da Paraíba, Campina Grande – PB * Corresponding author: [email protected] Introduction: The knowledge of the profile of drug use of the geriatric population is fundamental to the design of strategies for rational prescription of drugs for the elderly. Research of population-based can subsidize the professional development of health actions for improving drug therapy in these patients. Objectives: To describe the pharmacological profile of a population of diabetic elderly and dyslipidemic served in a Community Pharmacy in Campina Grande-PB. Materials and Methods: This was a cross-sectional study where 450 patients, consumers of drugs for chronic diseases in a Community Pharmacy answered a questionnaire based on the methodology Dáder and in sheet reporting of adverse reactions ANVISA. It was used Epi Info (version 6.0) for statistical analysis. Results and Discussion: Of the accompanieds patients, 12.0% used hypoglycemic and 8.0% used statins. Regarding to the antidiabetics, 68.0% used metformin (biguanide) and 38.0% used glibenclamide (Sulfoniluréia). Of the drugs used for dyslipidemia, the only available at the pharmacy, the object of this research was simvastatin. The average number of medications used was 4.0 drugs per patient. With relation to the treatment´s adherence, it found that most patients had a regular membership. It was observed that of the interviewed, 27.0% made use of self-medication. It also observed that the average of RNMs found in patients was 2.8 per patient. Conclusions: There was a high polypharmacy in patients interviewed, as well as possibles drug interactions in prescriptions, highlighting the importance of using some monitoring tools in care pharmaceutical that make possible a constant evaluation of these patients. Financial support from CNPq/Brazil and master scholarship from CAPES/Brazil.



205

CODE 194 EVALUATION OF HEMATOPOIESIS IN MALNOURISHED MOUSE

MODEL Pereira, PRS1; Silva, BCO1, Avelino, CUR1; Borelli, P2; Schimieguel, DM1 1 Laboratório de Hematologia Clínica; Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal do Rio grande do Norte (UFRN). 2 Faculdade de Ciências Farmacêuticas, Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo (USP). Corresponding author: [email protected] Introduction: Protein-energy malnutrition (PEM) is defined by WHO as a set of pathological conditions that result from a lower intake of protein and calories. The impact of PEM on hematopoiesis is associated with decreased cell production, leukopenia, anemia and immune system abnormalities. Objective: Evaluate parameters related to maintenance of hematopoiesis in a murine model of malnutrition. Materials and Methods: We studied 26 animals, 7 adult males and 19 females divided into two groups: malnourished M (n = 12) which was fed with low protein diet and control-C (n = 14), fed with normal diets. Samples of peripheral blood (SP) were used for assessment of blood counts and serum total protein and albumin. Samples of bone marrow (BM) were obtained by washing the cavity of femurs and tibias and used in clonogenic assays for enumeration of G-CFU and GM- CFU stimulated with growth factors and cytokines, and cell proliferation in 24h, 48h and 72h. Results and Discussion: Weight loss was observed significantly in group M compared to group C (p=0,0002). Hematological parameters showed lower hematocrit in group D (p=0,0025), hemoglobin (p=0,0086) and erythrocytes (p=0,0405). Clonogenic assays showed a significant decrease in scores of G-CFU (p=0,0386) of group D. Cell proliferation assays showed no differences between two groups in the three periods studied. Conclusion: Malnourished animals showed parameters that characterize malnutrition process, which corroborates current literature showed that hematopoietic progenitor cells was lower quantitatively and functionally due to delayed maturation of colony forming unit in vitro, affecting balance of hematopoiesis .



206

CODE 195 REPORTING OF DRUGS INDICATORS OF ADVERSE DRUG

REACTIONS: STUDY IN A UNIVERSITY HOSPITAL Oliveira, R. A.1; Pinto, C. O.1; Medeiros, J. W. T.1; Irineu, M. M.1; Barbosa, M. M. S.2; Brito, A. E.2 1 Academic of Pharmacy, University Hospital Onofre Lopes (HUOL), Federal University of Rio Grande do Norte (UFRN)

2 Pharmacist, University Hospital Onofre Lopes (HUOL), Federal University of Rio Grande do Norte (UFRN) Corresponding author: [email protected] Introduction: World Health Organization (WHO) defines adverse drug reaction (ADR) as "a response to a drug that is noxious and unintended and occurs at doses normally used in man for the prophylaxis, diagnosis or he py of d se se”. Abou 5% of hosp dm ss ons e due o ADR nd at least 15% of inpatients have adverse reactions. One can check if there are suspected ADRs by observing the prescription drugs used to treat them. Objective: Evaluate the importance of reporting of drugs indicators of ADRs by pharmacists. Materials and Methods: Data was obtained from the reporting of suspected ADRs from patients as well as the forms used for the investigation of them at University Hospital Onofre Lopes, a teaching hospital, tertiary, with 189 beds, in the period from October 2010 to July 2011. The ADRs were classified according to the type of notification (voluntary, active search and notification of drugs indicators) and according to the causality and severity. Results and Discussion: 45 adverse drug reactions were reported, of which 15 (33.4%) were reports of drugs indicators, 18 (40%) volunteers and 12 (26.6%) by active search. As for causality, 34 (75.5%) were probable, 9 (20%) possible, one (2.25%) was defined and one (2.25%) was doubtful. In severity, 31 (68.8%) were moderate, 9 (20%) mild, 3 (6.7%) severe and 2 (4.5%) lethal. Conclusion: Through the observation of drug indicators of ADRs, one can find a greater number of adverse reactions, which could often be imperceptible. Thus, there was a significant contribution to the increase in ADRs reporting.



207

CODE 196 STUDY OF TAQI/TGFΑ POLYMORPHISM IN NON-SYNDROMIC CLEFT

LIP AND/OR PALATE. Oliveira, G. H. M.1, Cardoso, M. L.1; Bezerra, J .F.1; Soares, C. D. 1; Silva, H. P. V.1; Silbiger, V. N. 1; Luchessi, A. D.1; Oliveira, A. M. L.1; Barbosa, A. C. S. 1; Fajardo, C. M.2; Hirata, R. D. C. 2; Almeida, M. G. 1; Rezende, A. A.1; Hirata, M. H. 2

1 Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil. 2 University of Sao Paulo (USP), São Paulo, SP, Brazil. Introduction: Nonsyndromic cleft lip patients with or without cleft palate (NSCL/P) are congenitally developmental disorders resulting from defects in growth and patterning of the facial primordial. TGFα is one of candidates genes suggested to be associated with NSCL/P. Objectives: The aim of this study was to standardize the methodology for High Resolution Melting – Real Time PCR for search the polymorphism TaqI/TGFα and evaluate allelic distribution in order to assess the association of alleles with development of oral clefts. Materials and Methods: 140 patients NSCL/P participated in this study. Blood samples were collected and genomic DNA was extracted and screened by HRM® - Real Time PCR (Applied Biosystems) and confirmed by sequencing the ABI 3730 DNA Analyser (Applied Biosystems), USP/SP. Results and Discussion: Most of the patients studied showed a high frequency of C1C1 genotype independent of the type of cleft. 65 individuals with Cleft Lip and Palate (CLP) (79.2%), 29 individuals with Cleft Palate (CP) (87%) and 23 individuals (92%) with Cleft Lip (CL) revealed this genotype C1C1. The heterozygote genotype C1C2 was present in 13 CLP individuals (15.5%), 4 CP individuals (5.3%) and only 2 CL individuals (8%). The C2C2 genotype was less frequent and only 4 patients with CLP had this genotype. Analyzing the alleles C1C1, C1C2 and C2C2 no significant difference statistically significant (P=0,375) associated with the type of cleft. Conclusions: The results identified the p esence of po ymo ph sm T qI n TGFα by HRM-Real Time in our patients although there is no association with the development of oral clefts. This study need be deepened for a better understanding of the genetic factors associated to NSCL/P in the population of Rio Grande do Norte. Financial support from FAPESP/Brazil and CAPES/Brazil



208

CODE 197 Phyllantus niruri L. REDUCE DAMAGE IN THE MUCOSAL IN MODEL

OF COLITIS Melo, M. N. 1; Soares, L. A. L.2 ; Júnior, R. F. A.3; Ferreira, A. A. A4; Teixeira, H. G. A.4; Camillo, C. S.3; Guerra, G. C. B.4 1Graduate Program on Pharmaceutical Sciences, Federal University of Pernambuco (UFPE); 2Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Pernambuco (UFPE); 3 Laboratory of Histology, Department of Morphology, Federal University of Rio Grande do Norte (UFRN); 4Laboratory of Pharmacology, Department of Biophysics and Pharmacology, Federal University of Rio Grande do Norte (UFRN); Corresponding author: [email protected] Introduction: The Inflammatory Bowel Disease (IBD) are chronic diseases, which manifest as Crohn's Disease and Ulcerative Colitis, and are characterized by alternating periods of intense intestinal inflammation and periods of remission. The etiology remains unknown, although it is believed that an alteration in the intestinal immune system contributes to the inflammation that occurs. As in other inflammatory processes, inflammatory bowel disease is characterized by an up-regulation in the synthesis and release of different pro-inflammatory mediators, including reactive oxygen and nitrogen metabolites, eicosanoids, platelet-activanting factor and cytokines. The classical treatments for IBD are based on drugs that act by inhibiting the production of prostaglandins and leukotrienes, such as aminosalicylates, and the use of immunosuppressive agents. Objective: To assess the activity of standardized dry extract of Phyllanthus niruri (SDEPn) in model of acute ulcerative colitis. Materials and Methods: Rats of the Wistar strain were used, which were divided into five groups: colitis (vehicle), group I (SDEPn 100mg/kg), group II (SDEPn 200 mg / kg), sulfasalazine group (500mg/kg) and non-colitis group (vehicle). Method was used to induce acute colitis with acetic acid 10%. The results were evaluated on macroscopic and histopathological findings. Results and Discussion: The rate of macroscopic damage and histological analysis have shown that preventive treatment with SDEPn significantly reduced the intensity of intestinal inflammation caused by acetic acid compared with the colitis group. Conclusions: The SDEPn exerts beneficial effects because reduce damage in the intestinal mucosal in model of acute colitis.



209

CODE 198 COMPATIBILITY BETWEEN CAPTOPRIL AND EXCIPIENTS THROUGH

THERMAL ANALYSIS Goes, J. S.1; Freire, F. D.1; Moura, T. F. A. L.1; Raffin, F.1 1 Laboratório de Desenvolvimento de Medicamentos, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Captopril was the first orally active potent and specific

inhibitor of the enzyme (ACE I), marketed in tablet form, which is adapted to the liquid form in order to be administered to children causing the loss of efficacy and safety of the drug. Objective: The objective of this study was

to evaluate the compatibility between the captopril and possible excipients (citric acid anhydrous, carboxymethylcellulose, hydroxypropylmethylcellulose, EDTA, potassium phosphate, methylparaben, propylparaben and saccharin) by thermal analysis to obtain a formulation captopril powder for reconstitution to be used as a pediatric dosage form. Materials and Methods: About 4 mg of isolates

and 1:1 binary mixtures were placed in alumina pans and analyzed on a Shimadzu DTG-60 apparatus, under nitrogen flow of 50 mL. min-1 at a heating rate of 10 °C. min-1 and the temperature range between 25 and 900°C. Results and Discussion: Through the DTA curve of captopril, we

determined an endothermic event at 110°C, corresponding to melting point of captopril. Between 170 and 440°C occurs the decomposition of captopril accompanied by weight loss. According to the curves TG, DTG and DTA, the excipients present in binary mixtures, for the most part, were compatible with captopril. The DTA curves of mixtures containing methylparaben and propylparaben suggest the presence of interaction between the captopril and preservatives such as the melting point occurred at 84°C and 76°C, respectively. Conclusions: Thus, we conclude these

excipients can be used in the formulation, but special attention should be taken to the performance of the formulations containing the preservative during the accelerated stability study.



210

CODE 199 INTESTINAL PARASITES IN CHILDREN AND FOOD MANIPULATORS

OF SCHOOL AND RESIDENTIAL OF A MUNICIPAL NURSERY IN JOÃO PESSOA-PB Pontes, Z. B. V.S.¹; Araújo, J.I. R.² Branco U.V.C.³; Júnior F.S.F.³; Vidal, E.P.4; Dantas I.F.4; Filho, W.L.N.²;Leão A.D.²; Freitas F.A.S.¹ ¹ Department of Pharmaceutical Sciences, Center for Health Sciences, Federal University of Paraíba (UFPB) ² Undergraduate in Pharmacy, Department of Pharmaceutical Sciences, Center for Health Sciences, Federal University of Paraíba (UFPB) ³ Pharmaceutical-Chemistry, Department of Pharmaceutical Sciences, Center for Health Sciences, Federal University of Paraíba (UFPB) 4 Basic Health Unit Nova Conquista(4), the Municipal Health Secretariat of Joao Pessoa-PB Introduction: Children still are the most damage beings by intestinal parasites, mainly because of their lack of hygiene, immunological development, and, depending on the location, they are submitted to a low basic sanitation range, and last but not least social and educational measures. Objective: To diagnose intestinal parasites on children, households, and school food of the municipal nursery Maria de Lourdes Gomes at Alto do Mateus in João Pessoa – PB. That way, promoting educational attitudes to the community, contributing to reduction of the prevalence of intestinal parasites, were the aims of this study. Materials and Methods: The parasitological analysis (methods of Hoffman and Faust) of 52 children between 3 and six years old, and 34 food manipulators were made; after an exempt and clarified approval, in the parasitological laboratory at the Universidade Federal da Paraíba. Results and Discussion: The fecal examinations of 52 children and 29 residential manipulators showed 71.2% and 62% respectively were parasitized. The existing school food manipulators not found to be infected. In children were isolated (multi replication) protozoa: Endolimax nana (41%), Giardia lamblia (25.5%), Entamoeba coli (17.6%), Entamoeba histolytica (14%) and Iodamoeba butschlii (1.9%) and / or helminths: trichiura trichiura (55.6%), Ascaris lumbricoides (33.3%), Enterobius vermicularis (5.6%) and Schistossoma mansoni (5.6%).The residential manipulators were parasitized, diagnosed with the following protozoa: : E. nana (45%), E. coli (20%), E. histolytica (12.5%), G. lamblia (12.5%) and I. butschlii (10%) and / or helminths, T. trichiura (40%), A. lumbricoides (30%), Ancylostomatidae (20%) and Trichostrongylus (10%).The frequency of intestinal parasites was high in children and manipulator show the need for public policies. Conclusions: The subjects are in a social context whose economic and sanitary conditions are precarious, necessitating the continuation of educational activities, diagnostic and treatment monitoring. Financial support from UFPB



211

CODE 200.1 CHARACTERIZATION OF SPRAY-DRIED PRODUCT AND

LIOPHILIZED DRIED EXTRACT OF Bauhinia forficata LINK BY INFRARED SPECTROSCOPY Lyra, M.A.M.1; Peixoto, M.S.1; Angelos, M.A.1*; Monteiro, R.P.M.; Costa, S. P.M1; Pina, E. M. L1; Santos, F. L. A1; Alves, L. D. S1; Souza, F.S.2; Rolim-Neto, P.J1. 1Universidade Federal de Pernambuco, Departamento de Ciências Farmacêuticas, Laboratório de Tecnologia dos Medicamentos, Cidade Universitária, Recife/PE, Brasil 2Universidade Federal da Paraíba, Departamento de Ciências Farmacêuticas, Brasil. Introduction: The plant species Bauhinia forficata Link (paw-to-cow) is widely used in the treatment of diabetes mellitus. Infrared spectroscopy (IR) is a technique of inestimable importance in qualitative organic analysis and is widely used in the area of chemistry of natural products, organic synthesis and organic transformations. Objective: Characterized by IR spectroscopy the spray-dried product and liophilized dried extract. Material and Methods: The IR spectrum of B. forficata samples was obtained in FT-IR spectrometer Spectrum 400 from PerkinElmer ® using an average of 10 sweeps from 650 to 4000 cm-1 with a resolution of 4 cm. Results and Discussion: The analysis of the IV absorption spectra of the raw vegetable material, spray-dried product and freeze-dried extract showed similar bands, indicating the presence of these constituents and that there were no changes in the spectra after drying the fluid extracts by different drying techniques . Conclusion: Therefore, we can infer that the drying processes are suitable for obtaining dried extracts for the development of solid dosage forms, however, it is necessary to analyze the samples by complementary analytical techniques and selective for this type of sample which has a mixture of constituents. Financial supporting from CNPq/FACEPE



212

CODE 200.2 EVALUATION OF INTERFERING LIPOPHILIC ON DETERMINATION OF

CONTENT OF TOTAL FLAVONOIDS BY SOLID PHASE EXTRACTION Peixoto, M.S1*; Lyra, M.A.M.1; Pina, E.M.L.1; Angelos, M.A.1; Ferraz, L.R.M.¹; Monteiro, R.P.M.1; Marques, G.S.1; Costa, S.P.M1.; Santos, F.L.A1; Rolim-Neto, P.J1. 1 Laboratório de Tecnologia dos Medicamentos, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE), Recife/PE, Brasil. Corresponding author: [email protected] Introduction: Bauhinia forficata Link is a native of South America, used in the treatment of diabetes mellitus due to antidiabetic activity attributed to flavonoid compounds present in the leaves. Objectives: Evaluate the presence of lipophilic interfering in determination of content of total flavonoids (CTF). Material and Methods: The effect of interfering lipophilic in the CTF of the extractive solutions was evaluated according to the alcohol content of liquid extractor, comparing the contents of the preparations at 50 and 70% (v/v) ethanol:water. O pretreatment of the sample aiming the retention of interfering lipophilic, was done by solid phase extraction (C18E 80mg/mL) and activated with 3 mL of hydro-alcoholic solution. The samples were analyzed by spectrophotometry according to the methodology developed and validated. The same procedure was repeated to obtain both fractions, without the addition of AlCl3, to obtaining of the compensationsolutions. The absorption spectra of the fractions in the range 200 to 500 nm were obtained 30 min after the addition of AlCl3.Results and Discussion:The extractive solutions of B. forficata with 50 and 70% ethanol showed a proportional increase of metabolites with higher alcohol content. However, this increase in the CTF is not sufficient to explain the lack of linearity obtained from the quantification of flavonoids by UV-Vis spectrometry and your marker, kaempferitrin, quantified by HPLC. Conclusions: However, it was evident that the extraction solutions analyzed showed no statistical differences in relation to CTF after the treatment on extraction column on solid phase, with no significant interfering lipophilic. Financial supporting from CNPq/FACEPE



213

CODE 201.1 PHYSICAL CARACTERIZATION OF SPRAY-DRIED PRODUCT AND

LIOPHILIZED DRIED EXTRACT OF Bauhinia forficata LINK BY SCANNING ELECTRON MICROSCOPY (SEM) AND X-RAY DIFFRACTION. Peixoto, M. S1; Lyra, M. A. M.1; Angelos, M. A.1; Monteiro, R. P. M.1; Marques, G. S.1; Pina, E. M. L.1; Lima, A. A. N1.; Souza, F. S.2; Rolim-Neto, P. J1

1 Laboratório de Tecnologia dos Medicamentos, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). 2Departamento de Ciências Farmacêuticas Universidade Federal da Paraíba (UFPB). Corresponding author: [email protected] Introduction: The plant species Bauhinia forficata Link (cow’s p w) s

widely used in the treatment of diabetes mellitus. Dried extracts have been used to obtain different dosage forms, in order to obtain products with higher concentrations of chemical constituents and with better technological properties. Objective: Caracterize spray-dried product and freeze-dried extract with analytical techniques. Material and Methods: It

was used a microscope Joel® JSM-5900 and a SIEMENS diffractometer with copper anode, with angle from 1,5 to 90cm with an interval of 2Ɵ and scan speed 0,02° 2Ɵ / s. Results and Discussion: SEM showed that the

extracts dried by lyophilization showed the presence of resinous aspect of continuous and irregular clusters. The spray-dried product had porous spherical particles with sizes between 1 and 10 micrometers. In X-ray diffraction, both dried materials showed to be typically amorphous, with the cen e of he mo phous pe k 2θ = 21,51º. Conclusion: Herbal dried

extracts are essentially amorphous and only spray dried product showed better defined particles, allowing a better flow during pharmacotechnical industrial development of solid dosage forms. Financial support from CNPq, FACEPE/ Brazil.



214

CODE 201.2 EVALUATION OF HYGROSCOPIC IN SPRAY-DRIED PRODUCT (SDP)

and LYOPHILISATE DRIED EXTRACT (LDE) OF LEAVES OF Bauhinia forficata Link Peixoto, M. S.1,2; Lyra, M. A. M.1; Angelos, M. A.1; Costa, R. P. M.1; Pina, E. M. L.1; Monteiro, R. P. M.1,3; Albuquerque, M. M.2; Rolim-Neto, P. J.1

1 Laboratório de Tecnologia dos Medicamentos, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). 2Núcleo de Controle de Qualidade de Medicamentos e Correlatos, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). 3Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). Corresponding author: [email protected] Introduction: The determination of the hygroscopicity in raw plant material

is an essay of great technological relevance for the production of herbal medicines. The high water content promotes the growth of microorganisms and influence pharmacotecnic quality of solid materials, which can affect the rheological characteristics and physical and chemical stability. Objective: To assess the hygroscopicity of SDP and LDE from Bauhinia forficata Link. Material and Methods: The extraction solution was

prepared in the proportion of 1:5 mass plant: volume of solvent, with 50% of hydroethanolic solution, heating at 40°C and stirring at 200 rpm for 1 hour. The SDP were obtained with 20 and 30% of colloidal silicon dioxide, while there was no addition of filler to the LDE. The hygroscopicity of the products was evaluated in a desiccator saturated with appropriate solutions (NaCl for RH 69% and BaCl2.2H20 for 88% RH) every 2 days for 14 days. Results and discussion: The SDP had lower moisture sorption that the

LDE. At RH 69% the moisture sorption was stabilized after 4 days, LDE sipped 20.06% and SDP 20% sipped 26.64%. After 14 days at RH 88% LDP drank 64.3% of moisture, SDP at 20% drank 47.66% and SDP at 30% drank 43.73%, illustrating a difference in performance of products when subjected to different humidity conditions . Conclusion: Among the

products analyzed, the SDP had lower hygroscopicity than the LDE, the latter requiring the addition of adjuvant drying to reduce moisture adsorption in plant materials.



215

CODE 202 SYNTHESIS, CHARACTERIZATION AND EVALUATION OF THE

LEISHMANICIDAL ACTIVITY OF MEGLUMINE ANTIMONIATE IN PROMASTIGOTE FORMS OF Leishmania major Omena, R. J. M.¹; Meneghetti, M. R.¹; Melo, G. M. de A. ¹; Cavalcante, A. Q.² ¹Grupo de Catálise e Reatividade Química, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas (UFAL). ²Laboratório de Farmacologia e Imunologia, Instituto de Ciências Biológicas e da Sáude, Universidade Federal de Alagoas (UFAL). Corresponding author: [email protected] Introduction: Leishmaniasis anthropozoonosis are considered a major public health problem. Ranked among the top five global parasitic diseases, leishmaniasis is present in 88 countries on five continents. For over six decades meglumine antimoniate (Glucantime®) is used as a drug of first choice for the treatment of leishmaniasis, despite its toxicity. Objectives: This work aims to determine our ability to synthesize, and evaluate the leishmanicidal and immunomodulating properties of organometallic compounds of antimony. Materials and Methods: For the syntheses of meglumina antimoniate, the methodology proposed by Demicheli et al (2003). The characterization was made by analysis of 1H NMR and infrared spectroscopies. To evaluate the leishmanicidal activity of meglumine antimoniate we have carried out feasibility testes with L. major promastigotes parasites in vitro. Results and Discussion: The data obtained from the 1H NMR and infrared analysis confirm the formation of meglumine antimoniate. The meglumine antimoniate failed to inhibit the growth of forms of L. major promastigotes in any of the concentrations tested. Conclusion: The synthesis methodology proposed by Demicheli et al (2003) was quite satisfactory, as well as being simple and rapid characterization demonstrated the formation of meglumine antimoniate. As already expected, the product obtained was not able to inhibit the growth of the parasite, but other biological essays are in progress.



216

CODE 203 INTERACTIONS OF ANTI-HYPERTENSIVE THERAPY DISPENSED BY

THE MUNICIPAL HEALTH NETWORK OF MACEIO / AL Nascimento, N. M.¹; Magalhães, Y. M.¹; Filho, A. D. O.1; Vasconcelos, H. A. L. M.1

1School of Nursing and Pharmacy, Federal University of Alagoas Corresponding author: [email protected] Introduction: The prescription of multiple medicines is a practice commonly used in therapeutic regimens. Interactions are special types of pharmacological responses, in which the effects of one or more medicines are altered by concurrent administration of other or earlier, or by concurrent administration with food. Several antihypertensive treatments using more than one medication, and there may be associations with other therapies, where patients may suffer the consequences of possible interactions between these medicines. Objective:To identify possible interactions of antihypertensive medicines present in the Municipal Register of Essential Medicines (REMUME), provided by the public health service of Maceió - AL. Materials and Methods: It was a literature review about the possible drug interactions between anti-hypertensive medications dispensed by the Basic Health Unit Graciliano Ramos-Maceió / AL to 198 hypertensive patients.Results and Discussion: The combined use of atenolol / propranolol and verapamil may depress myocardial contractility, causing severe hypotension. Non steroidal Anti-inflammatory medicines reduce the effects of thiazides, captopril, furosemide, and losartan. Captopril increases the risk of lithium toxicity. Enalapril increases in serum potassium. Furosemide causes kidney failure especially when associated with enalapril, captopril and losartan, and increased toxicity of salicylate. Thiazides affect on insulin requirements in diabetics, reduce effect of oral hypoglycemic agents. Methyldopa can lead to manifestations of idiosyncratic drug and verapamil increases serum digoxin levels and may result in digitalis intoxication, among others.Conclusions: Should be given greater attention as the prescription of combination therapy, because the resulting interactions can be numerous, both positive and potentially harmful and fatal to the patient.



217

CODE 204.1 USE OF STATISTICAL PROCESS CONTROL AS A TOOL IN

OPTIMIZING THE QUALITY OF THE PRODUCTION OF DIPYRONE 500 MG/ML ORAL SOLUTION DROPS Malheiro, J. P. T.2; Souza, E. de J.3; Oliveira, A. D. de1; Oliveira, A. R.2; Farias, S. R. Q de.1; Ramos, S. V. V.1; Oliveira, M. A. C. de1 ; Araújo, E. S. de.1; Vasconcelos, F. V. C. de1 1 Laboratório Farmacêutico do Estado de Pernambuco Governador Miguel Arraes (LAFEPE). 2 Universidade Estadual da Paraíba (UEPB). 3 Universidade Estadual de Feira de Santana (UEFS). Corresponding author: [email protected] Introduction: The search for quality assurance and a good performance of a production process, has fostered the need to use statistical methods as tools to optimize the preceding steps in the manufacture and establish measures to reduce losses, thus leading to improved processes productive. The Statistical Process Control (SPC) has been used for this purpose. Objective: Optimize the production process of the oral solution Dipyrone 500mg/mL drops. Materials and methods: Were analyzed 21 batches of Dipyrone 500mg/mL produced in the LAFEPE in 2009/2010, using the computer package WinCEP® plus 5, to establish the upper and lower control limit (UCL and LCL, respectively), for the parameters: content, pH, density and filling volume. Next, were analyzed three consecutive batches produced in 2010, comparing these results with the limits established by retrospective analysis. Results and discussion: The analysis of samples collected in the production process, all met the limits for content and density, 8.5% of samples (n = 4) were out of the LIC for pH, although 100% meet the specifications, and 50% remained above the LSC for the average volume filling, not characterizing a deviation from the requirements of the legislation. Conclusion: The study ensured that the production process of Dipyrone meets the quality attributes. The tools of the SPC revealed sensitivity to detect unusual events demonstrating that the variables pH and volume filling may be better adjusted to produce more uniform results.



218

CODE 204.2 ENVIRONMENTAL MONITORING IN THE FILLING LINE DROPS: AN

ANALYSIS OF THE ENVIRONMENT, PRODUCTIN PROCESS AND FINISHED PRODUCT DIPYRONE 500MG Malheiro, J. P.T.2; Souza, E. de J.3; Oliveira, A. D. de.1; Oliveira, A. R.2; Farias, S. R. Q de.1; Ramos, S. V. V.1; Oliveira, M. A. C. de1 ; Araújo, E. S. de.1; Vasconcelos, F. V. C. de1 1 Laboratório Farmacêutico do Estado de Pernambuco Governador Miguel Arraes (LAFEPE). 2 Universidade Estadual da Paraíba (UEPB). 3 Universidade Estadual de Feira de Santana (UEFS). Corresponding author: [email protected] Introduction: The quality and stability of a dosage form depends on their physical and chemical characteristics as well as the quality control of its manufacturing process. The presence of microorganisms in the product, may compromise the final quality or patient safety. In non-sterile, it admits the presence of microbial load, although limited, given the characteristics of its use. Objective: Evaluate the environmental conditions in the manufacture of Dipyrone 500mg/mL oral solution drops. Materials and methods: We analyzed 03 batches of Dipyrone 500mg/mL sequence drops produced in LAFEPE in October / November 2010, using as control variables of temperature, moisture, and microbial counts. This was done through Rodac Plates plates, enriched, broths, Petri dishes and air sampling, to the environment, and products in process and finished. Those who were considered critical points identified and monitored. Results and discussion: The variables temperature and humidity remained within specifications during handling and filling of the three lots in the study. The counting of environmental microbiological load in place of filling in the UFC, showed levels of contamination above the specified. However, analysis of the product in process and finished product, quantified in less than 10 UFC / mL micro-organisms, fungi and yeast, and identified the absence of pathogens in three batches. Conclusion: The liquid filling line control to maintain a good temperature and humidity. The microbial load in the environment, did not compromise the final product quality drops Dipyrone oral solution.



219

CODE 205.1 PRELIMINARY EVALUATION OF THE ANTINOCICEPTIVE ACTIVITY

OF THE SESQUITERPENE NEROLIDOL Carvalho, F. L.1; Fonsêca, D. V.1; Salgado, P. R. R.1; Leite, F. C.1; Sousa, D. P.2; Morais, L. C. S. L.1; Almeida, R.N.1 1Laboratório de Psicofarmacologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB); 2 Departamento de Fisiologia, Universidade Federal de Sergipe (UFS). Introduction: Medicinal plants are widely studied due to its therapeutic properties, attributed to the various chemical constituents synthesized by these plants, among which stands out the essential oils, as nerolidol for example. Nerolidol is a sesquiterpene with some pharmacological properties already described, such as antimicrobial, antifungal and anti-leishmania. Objective: This study aims to evaluate the antinociceptive activity of nerolidol using the acetic acid-induced writhing test. Material and Methods: Swiss male mice weighing 25-30 g were divided into groups of eight animals each and were treated orally (p.o.) with nerolidol (125, 250 and 500 mg/Kg), morphine (10 mg/Kg) and vehicle (Tween 80 and distilled water). After one hour of initial treatment, the animals received an intraperitoneal injection of acetic acid 1% to induce the abdominal contortions, characterized by abdominal constriction followed by extension of hind legs. During a period of 15 minutes were recorded the number of abdominal constrictions and latency to the onset of the first twist. The results were statistically analyzed by ANOVA followed by Dunn's multiple comparisons test, and the results were considered significant when p<0.05. Results and Discussion: Nerolidol was able to reduce significantly the number of abdominal contortions at doses of 250 (21.1 ± 3.1) and 500 (18.1 ± 2.0) mg/kg in the control group (47.8 ± 1.3). The latency to the first contortion was not different between the experimental and control groups. Conclusion: These results suggest that nerolidol has a possible antinociceptive activity. Further tests are needed to confirm this effect and to elucidate the antinociceptive mechanism. Financial support from CAPES/Brazil.



220

CODE 205.2 SEROLOGICAL STUDY OF CHAGAS DISEASE IN THE CITY OF

SALGADINHO/PB Carvalho, F. L.1; Fonsêca, D. V.1; Vilar, A. C. Q.2; Vilar, A. P. 2; Lima, C. M. B. L. 2; Freitas, F. I. S. 2 1 Laboratório de Psicofarmacologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB). 2 Laboratório de Parasitologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB). Introduction: Chagas disease was first described and discovered by the Brazilian physician Carlos Chagas in 1909. This infection is caused by the protozoan parasite Trypanosoma cruzi, whose most important vectors are the species Triatoma infestans, Rhodnius prolixus and Triatoma dimidata. Almost a century after its discovery, Chagas disease remains a serious public health problem in Brazil and Latin America. Objective: Given the lack of data on the number of seropositive individuals for Chagas disease in the city of Salgadinho, this study aimed to identify these patients and refer them for treatment. Material and Methods: The study had the participation of 215 volunteers, 74 from urban areas and 141 rural. Initially, educational lectures on various topics in health care were performed. After this step, blood was collected from these participants and submitted to the indirect hemagglutination and ELISA tests. Results and Discussion: From 215 volunteers, only 3 (1.4%) presented positive serology for both techniques applied. Women were more affected with the disease. All positive cases were aged between 51 to 60 years. The main occupation of patients with Chagas was agriculture. In relation to schooling, two patients had elementary school and one high school incomplete. Conclusion: The identification of infected patients for Chagas disease is of vital importance to no spread of this illness, making necessary the application of public measures to prevent new cases. Financial support from CAPES/Brazil.



221

CODE 206 EVALUATION OF PHYSICAL AND CHEMICAL COMPOSITION OF

THREE CULTIVARS OF PAPAYA SEEDS PRODUCED IN THE NORTHEAST OF BRAZIL FOR POSSIBLE RECOVERY IN FOOD INDUST Moreira, A. L.1; Carvalho, L. M.2; Costa, M. H3; Santos, N. A.4; Melo, M. L. S.1

1Department of Pharmaceutical Sciences, Federal University of Paraiba (UFPB). 2 Department of Food Engineering, Federal University of Paraiba (UFPB). 3Department of Industrial Chemistry, Federal University of Paraiba (UFPB). 4 Master in Chemistry from UFPB; Corresponding author: [email protected] Introduction: Brazil is the second largest producer of papaya and Paraíba occupies the fifth place in national production. Utilization of seeds to obtain a vegetable oil can use food and/or your pie for animal feed. Materials and Methods: We investigated the physical and chemical composition of seeds of three cultivars of papaya: Sunrise soil (Hawaii), Golden and Formosa. Consideration was also to oils obtained, as the fatty acid composition, physico-chemical and thermal stability. Results and Discussion: Cultivars Golden and Sunrise solo show in their physical composition a higher seed yield of 2.54 and 1.43% respectively against 0.615 cultivar Formosa. The chemical composition of the seeds in g/100 g showed a high protein content (23.58 to 25.46) fibers (16.07 to 19.19) and minerals (5.12 to 6.48) among the three cultivars, especially minerals Fe and P, with the greatest values, respectively, from 104.18 mg / g and 634.12 mg / g for cultivars Golden and Solo Sunrise. The oil content of three cultivars was ± 28% majority being oleic fatty acids with percentages of 69.78, 70.13, and 72.04% and palmitic acid with percentages of 18.95, 18.89 and 18.20, respectively for the Golden cultivar, soil and Formosa Sunrise. Indices in the same order of acidity: 1.0, 0.98 and 1.03, Iodine 71.30, 7.26 and 70.45 and saponification: 190,2,190,14 and 189.98. The test of thermal stability of the oil in an oven (65 ° C) for a period of 25 days showed a variation of peroxide value between (1 and 47.8 meq / kg). Conclusions: The results indicate the high nutritional potential of the pie and oil, whose chemical composition resembles qualitatively and quantitatively the olive oil, considered ideal for health maintenance.




222

CODE 207.1 EXTRACTION AND CHARACTERIZATION OF CHITIN FROM SHRIMP

WASTE OF RIO GRANDE DO NORTE - BRAZIL Santos, S. R. A.1; De Souza, I.1; Pedrosa, M. F. F.1; Fonseca, J. L.3; Silva-Júnior, A. A.1 1 Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN). 2 Department of Chemistry, Federal University of Rio Grande do Norte (UFRN). Introduction: Rio Grande do Norte (RN) is the both first and largest shrimp producer of Brazil. The main component of waste from shrimp is its shell, which is rich in composition of the polymer-β (1-4)-N-acetyl-D-glucosamine, usually called by chitin. Chitin may be used as flocculating agent in wastewater treatment, as adsorbent in clarification of oils and mainly for the production of chitosan, a biodegradable copolymer which has many industrial applications. Objectives: This study aimed to obtain and characterizing chitin in a laboratory scale from waste of shrimps produced in RN. Materials and methods: The extraction procedure of chitin was conducted in several steps: material collection, pretreatment, demineralization, deproteinization, deodorizing and drying. After obtaining the biopolymer, it was characterized by thermal analysis (Thermogravimetry - TG), X-ray diffraction, absorption spectroscopy in the infrared (IR) and scanning electron microscopy (SEM). Results and discussion: The thermogravimetric analysis curves showed three stages of degradation. An inorganic residual material content about 10.19% was identified. SEM micrographs of chitin showed a homogeneous, compact, fibrous and is consistent particles The X-ray analysis demonstrated the obtaining of a crystalline domains polymer. The characteristic stretching bonds at 3444cm-1 (OH axial stretching OH); 3270cm-1 e 3107cm-1 (NH axial stretching) were observed in IR spectrum. Conclusion: The physicochemical characterizing confirmed the obtaining of the polymer-β (1-4)-N-acetyl-D-glucosamine. Financial support from NEPGN, CNPq and PROPESQ-UFRN



223

CODE 207.2 EVALUATION OF THE AUTHENTICITY OF A SAMPLE OF SENNA

MARKETED IN NATAL-RN. Santos, S. R. A.1; Carvalho, J. R. P.1; Mesquita, P. C.1; Souza, I.1; Zucolotto, S. M.1 1 Laboratório de Farmacognosia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Senna alexandrina Mill (Leguminosae), popularly known as Senna, is widely used as a carminative, laxative, purgative and vermifuge mainly because of the presence of anthraquinones. Frequently, S. alexandrina is adulterated with other Senna species such as S. auriculata. Objetive: Then, the aim of this study was to verify the authenticity of a Senna sample marketed in the city of Natal, Rio Grande do Norte. Materials and Methods: The quality control analysis (identification, macroscopic and microscopic tests) of the Senna leaves were done according with 4th edition of the Brazilian Pharmacopoeia (1988). Chemical identification of anthraquinones was carried out by three methods: Bornträger Reaction, micro-sublimation test and Thin Layer Chromatography (TLC). Additionally, reactions to detect flavonoids and saponins were performed. Results and Discussion: In relation to the purity tests, the sample showed 1,2% of foreign materials (Pharmacopoeial standard: 5%). Macro and microscopic analysis showed similar results to the ones described in the Brazilian Pharmacopoeia, only the size of the leaves and the presence of essential oils was not in accordance. In the results obtained by chemical reactions, it was not detected the presence of tannins and flavonoids, only the presence of saponins and anthraquinones. In the TLC analysis, it was identified the senoside A and B, according with the color spots (brown-red) and Rfs (0.57 and 0.65, respectively) observed. Conclusion: According to our results, the commercial sample of Senna showed satisfactory results regarding the parameters established for identification of the drug plant in the Brazilian Pharmacopoeia, which contributes to the safe use of the drug plant.



224

CODE 208.1 EVALUATION OF QUALITY OF SAMPLES OF Baccharis trimera

MARKETED IN DIFFERENT CAPITALS OF THE BRAZILIAN NORTHEAST THROUGH HPLC ANALYSIS Angelos, M. A.1; Ferreira, P. A.1; Santos, F. L. A.1; Rolim, L. A.1; Alves, L. D. S.1; Lyra, M. A. M.1; Ferraz, L. R. M.1; Peixoto, M. S.1; Rolim-Neto, P. J.1

1 Laboratório de Tecnologia dos Medicamentos, Departamento de Ciências Farmacêutica, Universidade Federal de Pernambuco (UFPE). Corresponding author: [email protected] Introduction: Baccharis trimera, popularly known as Broom is a native

plant of South and Southeast regions of Brazil widely used in folk medicine against gastrointestinal problems. Among various secondary metabolites existent in the plant, flavonoids derivatives of caffeic acid stand out as they serve as chemical markers of the species. This study aims to determine the amount of caffeic acid by High Performance Liquid Chromatography (HPLC) of three commercial samples of "broom" from Recife, João Pessoa and Fortaleza. Objectives: To verify the legitimacy of commercial samples

obtained from "Broom" through the determination of caffeic acid by HPLC. Materials and Methods: The sample solutions were obtained from 1 g of

dried and milled drug performing extracts with ethanol: water (50:50) at a temperature of 40°C under stirring. The extracts were filtered through cotton. A rate of 1.2 mL was taken and diluted to 10 mL of an eluent system. The final solutions were injected into the equipment and the chromatograms were obtained with UV detector at 325 nm. Results and Discussion: The chromatograms obtained were similar to the profiles of

caffeic acid in the samples and pattern, differing only in the concentration of each one. Conclusions: The analysis of samples sold in different

markets demonstrated the legitimacy of products obtained from Baccharis trimera through the determination of caffeic acid, demonstrating the popular use of the correct species. The analytical methods used were appropriate for the determination of caffeic acid in the raw plant material. Financial support from CNPq, FACEPE/Brazil.



225

CODE 208.2 ANALYSIS OF EXTENDED RELEASE TABLETS BENZNIDAZOLE BY

RAMAN SPECTROSCOPY AND MICROSCOPY Angelos, M. A.¹; Lima, A. A. N.¹ ²; Almeida, E. C. L.³; Silva, C. C. A. R.¹; Lyra, M. M. L.¹; Santos, F. L. A.¹; Rolim Neto, P. J.¹ ¹ Laboratório de Tecnologia dos Medicamentos, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). ² Laboratório de Tecnologia Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas (UFAM). ³ Laboratório de Microscopia, Centro de Tecnologias Estratégicas do Nordeste (CETENE). Corresponding author: [email protected] Introduction: In drug development, the characterization of the raw material is needed, especially to know the physicochemical properties of the drug to optimize the development of the pharmaceutical form. The benznidazole (BNZ) is the only drug used to treat Chagas disease. Objective: To analyze the drug and tablets by Raman BNZ to check the influence of the drug and excipients in solid dosage form. Methodology: Was analyzed by Raman spectroscopy and microscopy, the BNZ, raw material and extended-release tablets of the drug, two lots of tablets, B1 (25% HPMC) and B2 (35% HPMC). Results and Discussion: In the analysis of the Raman spectrum of BNZ, is observed a broad band covering the whole spectrum, because the fluorescence of molecules in the compound that absorbs the laser excitation (514 nm). Two characteristic peaks are observed at 1130 cm -1 and 1360 cm-1. We observed the same peaks in the spectrum of tablet B1, noting a characteristic fluorescence, since there are other ingredients in the tablet that showed that fluorescence in the Raman spectra of each isolated component. This high fluorescence is more evident still in the Raman spectrum of compressed B2, where the highest concentration of this polymer further contributed to the high fluorescence observed in the spectrum. Conclusion: This procedure allowed obtaining micrometer-resolution spectra, important to evaluate the behavior of the drug alone and in front of the tablet excipients at different concentrations, demonstrating a promising technique in the evaluation of solid dosage forms. Financial support from CNPq, FACEPE/Brazil.



226

CODE 209.1 DETERMINATION OF KINETIC PARAMETERS BY OZAWA FOR

ALKALOID SYNTETIC WE010 Galvão, F. P.1; Silva, E. P.1; Júnior, J. X. de Araújo2; França, P. H. B.2; S n ’An , A. E. G.2; Aragão, C. F. S.1; Gomes, A. P. B.1

1 Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Universidade Federal de Alagoas (UFAL). Introduction: Thermal analysis is applied in the pharmaceutical industry for determination of the stability of a drug and formulations. The sample used in this analysis is the type guanilhidrazonas aromatic alkaloids. This alkaloids were prepared by direct reaction of the respective aldehydes with aminoguanidine hydrochloride in 95% ethanol reflux using an adaptation of the method described by Ulrich and Cerami (1984). Objective: The purpose of this study was to determinate by Ozawa model the following kinetics parameters: order, kinetic energy and frequency factor for WE010. Materials and Methods: The curves TG alkaloid WE010 were obtained in the thermobalance SHIMADZU, model DTG-60, using an alumina crucible, at 10, 20, 40, 60 and 80 ºC/ min–1 heating rate up to 900ºC, under an atmosphere of nitrogen at 50 mL/ min–1. It was weighted 7.0±0.5 mg. The curves TG were analyzed using TASYS program from Shimadzu, TGA Kinetic Analysis Program (Ozawa Model). Results and Discussion: The alkaloid WE010 was analysed by TGA kinetic analysis in the heating rate 10, 20, 40, 60 e 80 ºC min-1.The following kinetic parameters were found: order(0.4), kinetic energy (90.54 KJ/mol) and frequency factor(1.01 x 109 min-1) fo α – 0.2. The following kinetic parameters were found: order(0.3), kinetic energy (90.54 KJ/mol) and frequency factor(9.56 x 108 min-1) fo α – 0.3. Conclusions: Ozawa model equation can be used a suitable method for evaluate the stability of pharmaceutical, in this case in solid state. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN/Brazil.



227

CODE 209.2 THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE010 BY

TG/DTA AND DSC Galvão, F. P.1; Silva, E. P.1; Júnior, J. X. de Araújo2; França, P. H. B.2; S n ’An , A. E. G.2; Aragão, C. F. S.1; Gomes, A. P. B.1

1 Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN). 2 Universidade Federal de Alagoas (UFAL). Introduction: The application of thermal analysis, especially DSC

(Differential Scanning Calorimetry) and TG (Thermogravimetry), has been of fundamental importance in the study of characterization of the raw materials and pharmaceutical products, the purity determination and testing of stability and kinetics of decomposition. Objective: This study aims to thermal characterization, through curves TG/DTA and DSC of the alkaloid WE010. Materials and Methods: The alkaloid WE010 was analyzed by TG/DTA on a SHIMADZU thermobalance, model DTG-60, using an alumina crucible, at 10 ºC/ min–1 heating rate up to 900ºC, under an atmosphere of nitrogen at 50 mL/ min–1. It was weighted 7.0±0.5 mg. The DSC curves were obtained for SHIMADZU calorimeter, model DSC-60, using an aluminum crucible, at 10ºC/min-1 heating rate up 450ºC. The curves were analyzed using TASYS program from Shimadzu. Results and Discussion: The alkaloid WE010 showed four steps of decomposition. The first step corresponds the weight loss the humidy. The second step corresponds the decomposition and onset temperature was 211.36, with weight loss about 18%. The DSC data showed a melting peak, with onset 189.46 ºC, Heat 70.08 J/g and purity 97.97%. Conclusions: The thermal

techniques can be used in the characterization and purity determination of pharmaceuticals. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN/Brazil.



228

CODE 210.1 PARTICIPATION IN CLINICAL PAIN, FOLLOWING THE TREATMENT

OF ANTIINFLAMMATORY NON-STEROIDAL ONCOLOGY WARD ASSISTANCE FOUDATION OF PARAIBA – FAP Januário, M. D. A.1,2; Freitas, D. E.1,2; Morais, F. L. L.1,2; Quirino, T. F.1,2;

Santos, A. M. F.1,2; Sette, I. M. F.1; Belém, L. F1. 1 Department of Pharmacy, State University of Paraiba (UEPB). 2 Center of drug information, Campina Grande – PB. Corresponding author: [email protected] Introduction: The oncology develops, so very dynamic, and pharmacists are challenged to keep informed about new therapies for the development of an appropriate pharmaceutical care. It is important to the monitoring of hospitalized patients who make use of therapy with nonsteroidal anti-inflammatory drugs (NSAIDs), to be a pharmacological class of drugs widely used in hospitalized patients in order to provide greater patient comfort by relieving symptoms and especially pain and inflammation. Objective: To evaluate the use of NSAIDs in patients hospitalized in the oncology center promoting the rational use of medicines. Materials and Methods: Cross-sectional and quantitative approach in patients hospitalized in the oncology ward through the analysis of medical records. Results and Discussion: We analyzed 134 patients in the ward at the Hospital da Fundação Assistencial da Paraíba - FAP during the period January to July 2011. There are diverse types of diagnoses, the most prevalent cancer of the prostate (17%) and cervical cancer (14.8%). Of the 800 drugs used, distributed across 18 therapeutic categories, NSAIDs appear to 17.4% between these Dipyrone appears as the most prescribed NSAIDs most prescribed on the oncology ward with 95%. 9.7% of the patients studied had at least one RAM. Conclusions: The monitoring and drug safety are essential to the effective use of medicines and pharmaceutical care provision, particularly also in cancer patients, who due to their compromised physical state sets as a patient in risk to predispose Adverse Reactions Medicines (RAM). Financial support from CNPq/Brazil.



229

CODE 211 THE QUEST FOR ION CHANNEL MEMORY USING A LIPID

MEMBRANE Egito, E. M. N.1, 2; Escorel-Neto, A. C.2; Andrade, C. A. S.3; Cassia-Moura, R.1, 2, 4

1 Escola Politécnica, Universidade de Pernambuco (UFPE). 2 Laboratório de Modelagem Biológica, Instituto de Ciências Biológicas, Universidade de Pernambuco (UFPE). 3 Centro Acadêmico de Vitória, Universidade Federal de Pernambuco (UFPE). 4 International Centre for Theoretical Physics, Trieste, Italy. Corresponding author: [email protected] Introduction: Dynamic activation of ion channels formed by colicin Ia incorporated into a planar bilayer lipid membrane (BLM) was investigated by the voltage clamp technique using different step voltage stimuli. Objective: To control the dynamic activation of the ion channels by a specific sequence of pulses used for electrical stimulation. Materials and Methods: Lipid monolayers were formed using azolectin (L- -phosphatidylcholine type II - Sigma Chemical Co.) dissolved in hexane, so that solutions of 1% concentration were obtained. The aqueous solution consisted of 500mM KCl + 5mM CaCl2 + 5mM HEPES + 1mM EDTA (final pH 7.00). Colicin Ia was added directly to the aqueous solution in one half of the device attaining a final concentration of 1-electrodes were used to connect the equipment to the solutions (one electrode in each half) via salt bridges (2.5% agar in the chamber medium, electrodes immersed in 3M KCl). Results and Discussion: We have

c, between two identical

c, it produces a predictable current response. On the contrary, if the second

c, the current response cannot be reliably predicted. Conclusions: The behavior of the ion channels, when the

c, may be interpreted as a transient gain or loss or resetting of memory, as revealed by a specific sequence of electrical pulses used for stimulation. Financial support from CNPq/Brazil, FACEPE/Brazil; master scholarship from CAPES/Brazil.



230

CODE 213 PREPARATION OF DAPSONE-SILICATE NANOCOMPOSITES

Silva,T. A.1; Grillo, L. A. M.2; Dornelas, C. B.1

1Laboratório de Tecnologia e Controle de Medicamentos (LabTCoM), Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas (UFAL). 2Laboratório de Biociências Farmacêuticas (LabBFar), Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas (UFAL). Corresponding author: [email protected] Introduction: According to OMS, Brazil is the second country with the ges numbe of H nsen ’s d se se c ses. D psone s used as a drug of choice for its treatment, having a high permeability and low solubility in aqueous media, so currently formulations to increase its oral solubility are being sought. In this context, nanotechnology is already being successfully used. The aim of this work is the use of nanocarrier (silicate) associated with dapsone, aiming the improvement of the formulation. Objective: To prepare dapsone-silicate nanocomposites. Materials and Methods: The preparation of the nanocomposites followed the solvent method. Throughout the process, were evaluated: the type of silicate (organophilic or sodium clay), the dapsone-silicate relation (2:1,1:1,1:2) and the time of agitation (2, 24, 48 and 72hs) to establish the best condition. For that, the intercalation/adsorption efficiency indirect was calculated by spectrophotometry. Results and Discussion: In the studied conditions, the sodium clay yielded better results for dapsone than the organophilic clay. The result suggests that the used solvent, destilated water ph 2.0, leads to the drug ionization, with consequent increase in its affinity for the nanocarrier. Still, the best dapsone-silicate proportion was 1:2 by agitation for 24hrs, showing a 77.44% ± 2.85 intercalation/adsorption efficiency. After this time, in h s p opo on, he eff c ency doesn’ show ny s gn f c n increase. Conclusions: The results indicate the formation of sodium dapsone-clay composites with a relevant value of intercalation/adsorption efficiency. As a perspective, we intend to evaluate the type of the obtained nanocomposite by techniques such as XRD and TEM. Financial support from CNPq/Brazil.



231

CODE 214 INFLUENCE OF HIGH GLUCOSE CONCENTRATION ON BONE

METABOLISM IN MICE C57BL/6 INDUCED WITH HIGH-FAT DIET Bortolin, R. H.1; Santos, F. M.1; Oliveira, A. M. L.1; Freire-Neto, F. P.1; Bezerra, J. F.1; Morais, L. V. F.1; Ururahy, M. A. G.1; Barbosa, A. C. S.1; Lemos, B. S.1; Brito, F. A. P.1; Almeida, M. G.1; Himelfarb, S. T.2; Hirata, R. D. C.2; Hirata, M. H.2; Rezende, A. A.1

1 Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte (UFRN). 2 Departament of Clinical and Toxicological Analyses, University of São Paulo (USP). Corresponding author: [email protected] Introduction: T2DM is a progressive disease caused by both impaired insulin secretion and by insulin resistance. The diet-induced obesity in C57BL/6 has common characteristics of the human metabolic syndrome. Epidemiological studies have suggested that skeletal fragility is increased in T2DM. Objective: Evaluation of bone profile in male C57BL/6 mice induced to develop high glucose concentrations in a period of 32 weeks. Materials and Methods: Adults male C57BL/6 mice were divided in two groups: control (C, n=4) and diabetic induced by high-fat diet (DH,n=4). We evaluated body weight, capillary glucose concentration, activity of ALP total serum, and gene expression of RANK,RANKL,OPG PPAR-γ,IL-6,TNF-α and PTH in animal´s femurs. Results and Discussion: The result showed that body weight was increased for DH (p=1.0e-3) compared C and similarly capillary glucose concentration was increase in DH (p=3.0e-2) compared to C, indicating a possible induction of insulin resistance characteristic of T2DM. In bone metabolism evaluation the ALP activity for DH was increased when compared to C (p=2.4e-3) and genes expression of RANK,RANKL,IL-6 and TNF-α showed e ev ed o DH comp ed w h C (p=4.0e-2;p=3.0e-4;p=1.0e-2;p=1.0e-2 respectively), suggesting an onset of inflammatory process and unbalance on bone metabolism in favor of bone reabsorption. The PTH, OPG and PPAR-γ exp ess on d d not show significant difference. Conclusions: These results suggest that high fat-diet may increase the concentrations of glucose leading to an inflammatory process and consequently to possibly increasing bone reabsorption. Financial support from FAPESP and CNPq; Scholarship from CNPq and CAPES/Brazil.



232

CODE 215.1 CLINICAL AND EPIDEMIOLOGICAL ANALYSIS OF SCORPION

STINGS IN CAMPINA GRANDE: PART II

Costa, D. B.1; Melo, M. M. A.1; Cartaxo, N. A. O.1; Soares, N. S. C.1; Maris, S. R.2; Fook, S. M. L.1

¹ Toxicological information Service Center of Campina Grande (Ceatox – CG)/ Departamento of Pharmacy, State University of Paraíba (UEPB).

2 Ceatox – CG/ Health Sciences Academic Unit, Federal University of Campina Grande (UFCG). Corresponding author: [email protected] Introduction: Scorpionism is a public health problem in Brazil due to its high incidence rate in certain regions, and its ability to cause severe injury especially in children. Objective: Outline the epidemiological and clinical profile of scorpion stings notified at Ceatox-CG in 2009 and 2010. Methodology: Cross sectional retrospective study following a qualitative and quantitative approach. Data were collected through records provided by the Sistema Nacional de Notificação de Agravos (Sinan). Results: The sample considered 1168 patients, 61% (n=712) females. Prevalent age group consisted of individuals between 20 and 29 years old (20,5%). Most patients (88,8%) live in Campina Grande, 89,2% belonging to urban areas whilst 9,3% were in the countryside. The time elapsed between the bite and medical care was registered between 0 to 1 hour in 54,9% of the cases, being the foot the most frequent anatomic area affected (30,9% of the cases). A total of 97,3% of the patients presented local manifestations, 55,5% pain and 22,6% swelling. An amount of only 11,1% of the cases presented systemic reactions like headache (30%), vomiting (26,9%) and diarrhea (10%). Serotherapy was prescribed in eleven cases, nine being moderated and two being severe. All cases evolved to cure. Conclusion: Treatment in the first hours is critical in determining the severity of the cases. It is necessary to effectively combat the areas that enable the proliferation of the scorpion, especially the urban area, by raising public awareness through educational campaings.



233

CODE 215.2 SCORPIONIC AND OPHIDIAN ACCIDENT EPIDEMIOLOGIC PROFILE

NOTIFIED IN CAMPINA GRANDE CITY. Costa, D. B.1; Santos, R. L.¹; Fook, S. M. L.¹; Mariz, S. R.²; Melo, M. M. A.1; Costa, M. M. C.¹ ¹ Toxicological information Service Center of Campina Grande (Ceatox – CG)/ Departamento of Pharmacy, State University of Paraíba (UEPB).

2 Ceatox – CG/ Health Sciences Academic Unit, Federal University of Campina Grande (UFCG). Corresponding author: [email protected] Introduction: Accidents by snakes and scorpions are problems of Epidemiological Surveillance, Health Monitoring and Health Care. In Brazil, these accidents are mostly seen and reported by the Centers and Poison Information Service. Objective: To determine the epidemiological profile accidents between the years 2006 to 2008, in the Ceatox-CG. Methodology: Transversal retrospective study, quantitative and descriptive of all cases of snakebites and scorpion in Ceatox-CG, between the years 2006 to 2008. Data were collected through records provided by the Sistema Nacional de Notificação de Agravos (Sinan). The variables evaluated were: gender, age, area of occurrence, time between the event and the service and causing the specimens. Results: In the Analysed period, had notified 723 ophidian accidents cases and 948 scorpion stings cases. The ophidian accidents are prevalent in the rural area with 77% male gender habitants and 55% female gender. About the scorpionics accidents, it´s events that happen so often in the urban area being 93.7% male gender and 58.7% female gender cases. The ophidian and scorpions accidents it´s often in persons in the age of 20 to 29 years old. With respect to gravity are most lightweight type and, the patients are attended in the hospital unity until 3 hours. The responsible specimens for the big occurrence, it´s Bothropoides erythromelas (jararaca) e o Tityus stigmurus (scorpion). Conclusion: The obtained results in this research it´s tools that make possible a strategic formulation of prevention, improvement in the Health Assistance Services and notified systems of evaluation.



234

CODE 216 HYPERTENSION: EDUCATIONAL ACTIVITY IN PUBLIC HEALTH

Bastos, M. L. C1; Silva, M. C. M. da1; Campos, D. M.1; Cardoso, E. T. C.1; Luz, D. A. da Pinheiro, A. M1; Pontes, A. C. A. A1; Santos, C. C. dos1; Sarmento, R. M1; Silva, E. D. V; Silva, J. V. S.1; Silva, L. C. e1; Silva, M. L. da; Dolabela, M. F.2 1 Pharmacodinamic Laboratory, Federal University of Pará (UFPA). Corresponding author: [email protected] Introduction: The World Health Organization defined hypertension as "a disease characterized by a chronic elevation of systolic blood pressure and / or diastolic blood pressure"¹ The Ministry of Health of Brazil show that the cardiovascular disease are the first cause of mortality in the country² Because it is a public health problem of high prevalence and low control rates, and has risk factors (RF)³ the PET-Pharmacy bothered to do educational action with hypertension topic. Objective: Promove educational activities about Hypertension and its prevention. Materials and Methods: The activity in UBS in a neighborhood on the outskirts of Belém city of was held with formulary designated to population for check your knowlege on topic and guidance with brochure explanatory delivering. Results and Discussion: Of 36 patients, 72,2% c m o know “wh is hype ens on”, 33.3% are unaware of their symptoms and only 22.7% know more than three symptoms. Related to RF, 75% are unaware and 15% "somewhat knowledgeable" less than 35% are aware of more than three factors. About controlling h gh b ood p essu e, “ e know edge” were aware, because of the 58.33% who said they know how to keep constant, the majority cited the reduction of salt as a factor in improving treatment. Conclusion: It was apparent the need for knowledge about hypertension, which is worrying, as 66.6%respondents have relatives with hypertension, which worsens when only 25% say they receive guidance from health professionals. Was obtained orientation and collaboration of population by generating discussion questions on the subject. Financial support from PROEG,UFPA/Brazil.



235

CODE 218.1 ANALYSIS OF DIFFERENT BATCHES OF BENZIDAZOLE BY HPLC

AND FTIR Ferraz, L. R. M.1; Lima, A. A. N.1,2; Santos, F. L. A.1; Lyra, M. A. M.1; Soares-Sobrinho, J. L.1; Rolim-Neto, P. J.1 1 Laboratório de Tecnologia dos Medicamentos, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). 2Laboratório de Tecnologia Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas (UFAM). Corresponding author: [email protected] Introduction. The benznidazole (BNZ) is an antiparasitic drug used in the treatment of Chagas disease. The characterization of the active ingredient should be the first step in obtaining pharmaceutical forms. One of the echno og c bo enecks n h s ob n ng ’s he d ffe ence be ween batches of physical-chemical properties inherent to the drug. Objective. The aim was to characterize the different batches and investigate possible differences between them, using the techniques of Fourier Transformed Infra-Red (FTIR) and High Performance Liquid Cromatography (HPLC) as identification methods. Materials and Methods. Was analyzed, by FTIR and HPLC, the BNZ three different batches. The LC method involved a C18 column and a mobile phase comprising of ACN:water (1:1 v / v) at 1mL.min-1 (321m). Results and Discussion. The spectrogram was analyzed relating the characteristic peaks, particularly the typical bands of the amide (N-H stretching vibration), carbonyl stretching (amide I band) and N-H deformation (amide II band), and the vibrations due to the benzyl group and imidazole. The results of the HPLC chromatograms showed a characteristic chromatographic peak of BNZ in a retention time around 4.1 minutes, for the 3 lots analyzed. Was also determined the purity of the three lots, which was 99.99%. Conclusions. The analysis of FTIR served as a test for identification of the lots, proving that it is the same substance, due to all the bands was placed on the same wavelength. The results obtained by HPLC showed that each lot has the quality assured and are ready for the development of pharmaceutical forms. Financial support from CNPq and FACEPE/Brazil.



236

CODE 218.2 UTILIZAÇÃO DE TÉCNICAS ESPECTROMÉTRICAS PARA A

CARACTERIZAÇÃO DO 3-(4-CLORO-BENZIL)-5-(4-NITRO-BENZILIDENO)-IMIDAZOLIDINA-2,4-DIONA (LFPS/FZ4) Ferraz, L. R. M1; Costa, S. P. M.1; Medeiros, G. C. R1; Maia-Junior, R. T1; Rolim, L. A1; Rolim-Neto P. J1; Silva K. E. R. 1; Lima M. C. A. 3, Pitta I. R.3; Galdino S. L.3

1 Laboratório de Tecnologia dos Medicamentos (LTM), Departamento de Farmácia, Universidade federal de Pernambuco (UFPE). 2 Faculdade de Farmácia da Universidade Federal do Pará (UFPA). 3Laboratório de Planejamento e Síntese de Fármacos (LPSF), Departamento de Antibióticos, Universidade federal de Pernambuco (UFPE). Corresponding author: [email protected] Introduction. The prototype 3-(4-chloro-benzyl)-5-(4-nitro-benzylidene)-imidazolidine-2 ,4-d one (LPSF/FZ4) ’s n m d zo d ne de v ve nd s a part of a huge class of bioactive compounds that presents an important activity schistosomicidal. Objectives. The objective of study was to characterize by spectrometric techniques a novel molecule (LPSF/FZ4) aiming at the standardization of raw materials. Materials and Methods. The LPSF/FZ4 (Batch: 1, 98.00%) was provided by Laboratório de Síntese de Fármacos - UFPE. Spectrometric analysis were performed in the infrared (IR), in the ultraviolet (UV), hydrogen nuclear magnetic resonance (1H NMR) and mass spectrometry (MS / IT-TOF). Results and Discussion. The IR data identified the presence of major functional groups: the typical bands of amides (1685 cm-1 and 1661 cm-1) representing the imidazole ring present in the molecule that gives the anthelmintic activity in addition to the nitro group, considered parasitophorous, observed at positions between 1543 cm-1 and 1342 cm-1. The structural elucidation by analysis of 1H NMR was complementary to IR, identifying the hydrogens of the aromatic groups that presents chemical shifts δ in the range of 7.3 to 8.2, which confirms the position para of the substituents to the molecule. The molecule showed a specific pattern of absorption in the UV-Vis at 346nm. The mass spectrum was peculiar, and can detect the gross mass of the prototype in [M-1] = 356.0250 m/z. Conclusions. Therefore, the characterization was of fundamental importance, because it allowed the structural elucidation of LPSF/FZ4, providing relevant information for rational drug design. Financial support from CNPq and FACEPE/Brazil.



237

CODE 220 IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY OF

ENTEROCOCCI ISOLATED IN THE CITY OF NATAL / RN, BRAZIL Costa, M. K. C. M.1; Melo, M. C. N.2; Cidral, T. A.2 ; Silva, S. S. P.2 ; Merquior, V. L. C.1

1 Laboratório de Bacteriologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de janeiro (UERJ). 2 Laboratório de Bacteriologia Médica, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Enterococci are ubiquitous organisms, common gastrointestinal and genitourinary tract. However, they are opportunistic pathogens causing mainly urinary tract infections, bacteremia and endocarditis.Objective: Identify isolates of enterococci at the species level and evaluate the susceptibility to antimicrobial agents commonly used in clinical practice. Materials and Methods: Were analyzed 68 clinical samples, collected from 04 hospitals in the city of Natal/RN, Brazil, from October 2010 to June 2011. The identification at the species was made by the conventional tests. Evaluation of susceptibility to most antimicrobials was performed by disk diffusion method (CLSI, 2011) and vancomycin resistance by E-test. The antimicrobials tested were: vancomycin, teicoplanin, ampicillin, tetracycline, fosfomycin, ciprofloxacin, and high levels of aminoglycosides (gentamicin/HLR and streptomycin/HLR). Results and discussion: Of the 68 enterococci isolates, 63 (92.65%) were identified as Enterococcus faecalis and 05 (7.35%) Enterococcus faecium. In relation to antimicrobials was detected low percentage of resistance to ampicillin (1.47%), fosfomycin (1.47%) and gentamicin/HLR (8.82%) and the higher percentage for ciprofloxacin (66.17%), tetracycline (54.41%) and streptomycin/HLR (32.35%). For vancomycin there was resistance from 11,76%. However, by E-test were sensitive. The high antimicrobial sensibility observed is due to the fact that E. faecalis is still a very sensitive species, but it has a high potential of virulence. Conclusions: The identification at the species levels and resistance pattern of these isolates are important for establishing the epidemiology of infections this microorganism in hospitals surveyed well as, to assist possible preventive measures to control infections clinically important. Financial support from master scholarship from CAPES/Brazil.



238

CODE 222 HUMAN LEUKOCYTE ANTIGEN-G AND -E EXPRESSION IN GASTRIC

SPECIMENS IN PATIENTS WITH Helicobacter pylori Souza, D. M. B. O.1; Reginaldo, F. P. S.1; Giabardo, T. 2; Soares, C. P.2; Rocha, K. B. F.3; Oliveira, C. N.3; Jatobá, C. A. N.3; Donadi, E. A.4; Crispim, J. C. O.1

1 Department of Toxicology and Clinical Analysis, School of Pharmaceutical Sciences, (UFRN). 2 Department of Clinical Analysis, School of Pharmaceutical Sciences, University of São Paulo State (UNESP). 3 Department of Pathology, School of Medicine of University Federal Rio Grande do Norte State (UFRN). 4 Division of Clinical Immunology, School of Medicine of Ribeirão Preto, University of São Paulo (FMRP-USP). Corresponding author: [email protected] Introduction: Human leukocyte antigen-G and -E (HLA-G and HLA-E) are a non-classical major Histocompatibility complex class Ib molecules that acts as a specific immunosuppressor. Some studies have demonstrated which HLA-G and HLA-E seems to be involved in inflammatory diseases, cancer and viral infections. However, yet nothing is known about the relationship of these molecules in bacterial infections. The present study is the first to explore the HLA-G and HLA-E expression profile in gastric tissue of H. pylori -infected patients. Objective: Determine if HLA-G and HLA-E are expressed in gastric specimens and if the expression is associated with clinicopathological data involved in H. pylori infection. Materials and Methods: We performed a cross-sectional study, systematically comparing the qualitative expression of the HLA-G and HLA-E in individuals harboring H. pylori correlating with clinical variables. Fifty biopsies of the patients with a diagnosis of infection by H. pylori were evaluated by immunohistochemistry using the primary monoclonal antibody anti-HLA-G and anti-HLA-E (EXBIO). Results and Discussion: We detected HLA-G molecules in 46 cases (92%) of 50 patients with H. pylori. HLA-E molecules were detected in 42 cases (84%). 50 (100%) specimens presented chronicity, 49 out of 50 (98%) presented changes in repair, 45 out of 50 (90%) presented inflammatory activity. Conclusions: HLA -G and -E status plays an important role in the clinical outcome of H. pylori infection. These findings are important; however, additional studies are necessary to investigate the possible involvement of the molecules HLA-G and HLA-E in the pathogenesis of infection with H. pylori.



239

CODE 223 COST-EFFECTIVENESS OF ANTIHYPERTENSIVE THERAPIES IN

BASIC HEALTH SYSTEM Silva, J. P. R.1; Félix, B. F.1; Lacerda, D. P1; Batista, R. S. de A.1; Ferreira, E. V. G.1; Barros, A. R. A.1; Barbosa, F. O.1; Viana, M. de S.1; Soares, R. A.1; Silva, P. H. A. da 1; Alves, H. da S.1; Queiroz, I. R. de 3; Oliveira, M. F. de3. 1 State University of Paraiba (UFPB). 2 Federal University of Campina Grande (UFCG). 3 Nurses, Basic Health System, City of Campina Grande-PB Corresponding author: [email protected] Introduction: Systemic Arterial Hypertension (SAH) is a multifactorial clinical condition characterized for high and sustained levels of Blood Pressure (BP). It is commonly associated to functional alterations and/or structural alterations in specific organs (heart, brain, kidneys and blood vessel) and metabolic alterations, with a bigger cardiovascular risk, fatal or not. SAH has a high prevalence, reaching 22, 3% to 43, 9% of Brazilian population and it is responsible for high medical and socioeconomic coasts. Objective: To evaluate cost-effectiveness of antihypertensive therapies in basic health system. Materials and Methods: Transversal study, realized from July to September, 2011, in two public basic health unities, in Campina Grande, Paraíba. 359 hypertensive patients were evaluated. The Blood Pressure was obtained and evaluated by Brazilian guidelines. Data was typed twice and analyzed by the use of software Epi Info 3.5.2. Results and Discussion: After analyzing data, 72% patients were in polytherapy and 56% had BP controlled satisfactorily. The most used therapeutic diagram were association between diuretic and angiotensin-converting enzyme inhibitors (ACEI) and monotherapy with diuretic or ACEI. The mensal coast with therapy was R$ 757,00. Conclusions: As the patients are in the third age and use polytherapy, analyzing the farmacotherapy is necessary and correcting some problems related to medications, wich can result in collateral reactions and bad control of BP levels, increasing the risk of a cardiovascular disease.



240

CODE 224.1 SURVEY OF RISK FACTORS FOR PREVALENCE OF CASES OF TYPE

2 DIABETES MELITTA IN THE CITY OF ARARA-PB Freitas, W. S.1; Alves, R. D.1; Alves, L. D.2; Abreu, V. M.1; Pedrosa, A. R. S.1; Pedrosa, R. S.1; Venceslau, E. M.3; Filho, G. V. P.4

1 Departamento de Farmácia, Universidade Federal de Campina Grande (UFCG). 2 Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 3 Departamento de Enfermagem, Universidade Federal de Campina Grande (UFCG). 4 Graduado em Farmácia. Corresponding author: [email protected] Introduction: Diabetes mellitus (DM) is a chronic metabolic disease characterized by high blood glucose (hyperglycaemia) resulting from defects in secretion and or insulin action, affecting much of the population worldwide and is associated with morbidity and responsible for acute and chronic complications. It is worth noting that most of the subjects with DM, unaware of their condition, and thus do not perform any type of treatment. Objective: This study aimed to evaluate the quality of life of patients with diabetes who live in the city of Arara, PB, listing the key risk factors. Materials and Methods: According to data from the municipality HIPERDIA there are about 221 diabetic patients enrolled in the program, they receive monthly monitoring by primary care through medical and nursing, and made periodic visits by Community Health Agents (CHA). It a study of exploratory and descriptive approach with qualitative and quantitative, which were made periodic visits to health clinics and watched closely as they were made the calls from patients. Data were collected from January to March 2011, using the free and systematic observation. Results and Discussion: The results showed that the prevalence of the disease is directly related to risk factors, where the eating habits and physical activity exerts a powerful influence on the energy balance. Conclusions: Diets with high energy density associated with a sedentary lifestyle are appointed as the main etiological factors of the increasing prevalence of obesity in the world.



241

CODE 224.2 EPIDEMIOLOGICAL SURVEY IN CASES OF HEPATITIS TOWN OF

CUITE - PB, BRAZIL, 2011 Freitas, W. S.1; Alves, R. D.1; Alves, L. D.2; Abreu, V. M.1; Pedrosa, A. R. S.1; Pedrosa, R. S.1; Venceslau, E. M.3; Filho, G. V. P.4

1 Departamento de Farmácia, Universidade Federal de Campina Grande (UFCG). 2 Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 3Departamento de Enfermagem, Universidade Federal de Campina Grande (UFCG). 4 Graduado em Farmácia e Secretário Municipal de Saúde de Cuité-PB Corresponding author: [email protected]

Introduction: Viral hepatitis comprises a serious public health problem, both at the level of Brazil, the world and is thus considered an important public health issue. There are at least six virus, named A through E, G, and a newly discovered virus. Primary care teams have an important role in the diagnosis and monitoring of people with hepatitis, the major route of transmission of the virus is fecal-oral, inter-human contact or through contaminated food and water. The spread is related to the socioeconomic status of the population, there are regional variations in endemicity according to the degree of sanitation, hygiene education and hygiene conditions of the population. Objective: The study aimed to survey a given unit of health of the city of Cuite-PB approached a profile of incidence of this problem in this region, being an enabling environment for expression of this virus. Materials and Methods: The methodology was based on quantitative research, conducted from January to June 2011. Results and Discussion: The Municipal Health observed that this region had only two cases of viral hepatitis, although the region is little developed and people are more exposed the virus. Conclusions: Epidemiological surveillance of viral hepatitis in Brazil uses the universal system and liabilities, based on the reporting of suspected cases of confirmed cases and outbreaks of viral hepatitis, so it should be noted that this reporting is being done correctly to see whether the data collected is in fact the index profile of hepatitis in the city of Cuite.



242

CODE 225 USE OF BABASSU IN THE ADSORPTION OF CHEMICALS FOR

WASTE TREATMENT IN INDUSTRY CHEMICAL AND PHARMACEUTICAL Silva, G. M. S1; Lima, H. R. S.1; Cruz, E. T. L.1; Nunes, L. C. C.2 1Laboratory of pharmaceutical technology, Department of Pharmacy, Federal University of Piauí (UFPI). 2 Nucleus of Pharmaceutical Technology, Department of Pharmacy, Federal University of Piauí (UFPI). Introduction: The simple presence of residual chemicals/pharmaceuticals in the aquatic environment can cause noticeable adverse physiological effects in humans and animals. In view of this, needs some methods to try control its elimination, among these methods highlight the adsorption. Objective: Due to the adsorption capacity, as reported, of the derivatives of babassu and its geographical abundance, this study was carried out in order to verify the adsorption capacity of the mesocarp, coal and activated charcoal from babassu. Materials and methods: For preparation of activated carbon, took a sample of crushed coal, added phosphoric acid 0,1 N later the system was neutralized with potassium hydroxide 0.1 N and for full activation, took it to the hothouse at 85 ° C. Each procedure lasted 24 hours and always under agitation and changing a procedure for the other held a filtration and a wash to stabilize the pH. To check the adsorption of activated charcoal compared to the crushed coal and babassu, took an aliquot of each component and added a standard solution of methylene blue, agitating the new systems for 24 hours. Then, filtered systems and performed the spectrophotometric scan. Results and discussion: The potential for adsorption of the three systems was growing. According to the spectrophotometric reading, the system with babassu mesocarp resulted in a percentage adsorption equal to 89%, the system containing crushed coal, 94% and containing activated charcoal, 97%. Conclusion: The activated charcoal from babassu is a powerful adsorbent and may be useful in cleaning up industrial waste. Financial support from CNPq/Brazil.



243

CODE 226 HUMAN LEUKOCYTE ANTIGEN-G AND TH17 EXPRESSION IN

CERVICAL INTRAEPITHELIAL NEOPLASIA Miranda, L. N.1; Soares, E. G.2; Souza, D. M. B. O.1; Oliveira, A. E. F.1; Rocha, K. B. F.3; Soares, C. P.4; Silva, T. G. A.4; Donadi, E. A.5; Crispim, J. C. O.1 1 Department of Toxicology and Clinical Analysis, School of Pharmaceutical Sciences, University Federal Rio Grande do Norte (UFRN). 2Department of Pathology, School of Medicine of Ribeirão Preto, University of São Paulo, (FMRP-USP). 3 Department of Pathology, School of Medicine of University Federal Rio Grande do Norte (UFRN). 4 Department of Clinical Analysis, School of Pharmaceutical Sciences, University of São Paulo (UNESP). 5 Division of Clinical Immunology, School of Medicine of Ribeirão Preto, University of São Paulo (FMRP-USP) Corresponding author: [email protected] Introduction: Cervical intraepithelial lesions are one of the most frequent causes of cancer among women worldwide. The discovery of early diagnostic markers has been a central subject. Human leukocyte antigen-G (HLA-G) is a non-classical major histocompatibility complex class Ib molecule that acts as a specific immunosuppressor. A new class of T cells production IL-17 has been identified which plays a critical role in numerous inflammatory conditions and autoimmune diseases. The present study is the first to explore the HLA-G and IL-17 expression profile in cervical biopsies. Objective: Determine if HLA-G and IL-17 are expressed in cervical biopsies with intraepithelial cervical neoplasia. Materials and methods: We have evaluated the expression of the HLA-G and IL-17 by immunohistochemistry in 71 cervical histological samples including 38 low-grade cervical intraepithelial neoplasia (NIC I), 4 high-grade cervical intraepithelial neoplasia (NIC II and NIC III) and 29 only with inflammation. Results and discussion: HLA-G molecules were detected in 38 cases (53,5%). Among specimens that presented HLA-G expression, 24 (33,8%) was classified as NIC I, 2 (2,8%) classified as NIC II and NIC III and 12 (16,9%) classified as inflammation. In relation to IL-17 cytokine were detected in 55 cases (77,3%); 31 (43,6%) was classified as NIC I, 2 (2,8%) was classified as NIC II and NIC III and 22 (30,9%) classified as inflammation. Conclusions: In this review we focus utility of specific biomarkers in identifying true cancerous precursor lesions, additional studies are necessary to identify the influence of HLA-G and IL-17 expression on cervical neoplasia.



244

CODE 227.1 POSSIBLE ANTINOCICEPTIVE EFFECT OF Lippia microphylla

(VERBENACEAE) ESSENTIAL OIL IN MICE Penha, A. R. S1; Mota, V. G.1; Monte, L. O.1; Carvalho, F. L.1; Tavares, J. F.1; Morais, L. C. S. L.1; Alencar, J. L.2; Almeida, R. N.1 1 Laboratory of Pharmaceutical Technology, Center of Health Sciences, Federal University of Paraiba (UFPB). 2 Department of Internal Medicine, Federal University of Paraiba (UFPB). Corresponding author: [email protected] Introduction: The natural products including medicinal plants have been established as a main source for obtaining new drugs with potential therapeutic effect. Species of the Lippia genus are widely used in folk medicine for treating pain and other ailments. Objective: This study investigated the antinociceptive effects of the Lippia microphylla essential oil (LMEO) in animal models. Materials and Methods: All experimental protocols were approved by the local Ethics Committee for Animal Research (CEPA Nº: 0304/08). Swiss mice, albino, male, weighing between 30-40g, 3-month-old, divided into groups (n= 8 animals) were treated intraperitoneally (i.p.) to perform: 1) formalin test1 and 2) hot plate test1: LMEO (50, 100, 150 mg/kg), vehicle, 10 mg/kg morphine. Results and Discussion: In the formalin test, mice treated with LMEO in the three doses tested (50 mg/kg: 44.3 ± 5.8; 100 mg/kg: 26.6 ± 2.2; 150 mg/ kg: 12.7 ± 4.6 s.) decreased the licking-time in the first phase of the test, compared to control animals (65.2 ± 5.5 s.). In the second phase, doses of 50 mg/kg (64.7 ± 25.2 s.), 100 mg/kg (30.3 ± 18.1 s.) and 150 mg/kg (7.3 ± 7.3 s.) reduced the licking-time compared to control (139.3 ± 17,4 s.). The time of response to the thermoceptive stimulus in hot plate test was longer in LMEO-treated animals (150 mg/kg) (10.2 ± 2.0 s.) than control group (4.1 ± 0,8 s.) at 30 minutes. Conclusions: LMEO presents psychopharmacological effects suggestive of psycholeptics drug with promising antinociceptive activity, indicating that this activity may probably be mediated in the level of CNS. Financial support from LTF, CNPq/Brazil.



245

CODE 227.2 INVESTIGATION OF ACUTE TOXICITY AND MIORRELAXANT EFFECT

OF Lippia microphylla (VERBENACEAE) ESSENTIAL OIL IN MICE

Penha, A. R. S1; Mota, V. G.1; Monte, L. O.1; Carvalho, F. L.1; Tavares, J. F.1; Morais, L. C. S. L.1; Alencar, J. L.2; Almeida, R. N.1 1 Laboratory of Pharmaceutical Technology, Center of Health Sciences, Federal University of Paraiba (UFPB). 2 Department of Internal Medicine, Federal University of Paraiba (UFPB). Corresponding author: [email protected] Introduction: The genus Lippia has yielded a great number of medicinal and economically important species that are frequently used in folk medicine. Psychopharmacological effects, such as sedative and myorelaxant, have already been characterized for some species3,4. Objectives: This study investigated the acute toxicity and the myorelaxant effect of the Lippia microphylla essential oil (LMEO) in animal models. Matherial and Methods: Swiss male mice (30-40g, 3 months old) were divided into groups (n=8-10 animals) and treated intraperitoneally (i.p.) to perform: 1) LD50

1: 100, 200, 300, 400, 500, 1000 mg/kg LMEO (used as reference for the choice of relatively safe doses for the subsequent tests) and 2) Rota rod test2 : vehicle, 50, 100 and 150 mg/kg of the LMEO and diazepam (2 mg/kg). All experimental protocols were approved by the local Ethics Committee for Animal Research (CEPA Nº: 0304/08). Results and Discussion: The LMEO lethal dose 50% was calculated by nonlinear regression and this was estimated at 385.3 mg/kg with confidence limits from 298.2 to 497.8 mg/kg. Thirty minutes after treatment with LMEO, animals treated with a dose of 150 mg/kg showed a significant decrease in time spent in the revolving bar at rota rod test (113.8 ± 19.8 seconds versus 179.1 ± 0.8 seconds of control group). The other tested doses did not promote significant changes in this parameter. Conclusions: These results show that LMEO present low DL50, which may be an indicative of toxicity, but it did not affect muscular coordination in mice, except in the first minutes after treatment with higher doses. Financial support from CNPq/Brazil.



246

CODE 228 CHARACTERIZATION OF THE OPUNTIA FICUS-INDICA (L.) MILL

DRIED EXTRACT BY THERMAL ANALYSIS AND INFRARED SPECTROSCOPY Souza, C. M. P.1,2; Soares, B. S. A.2; Santos, D. P. G.2; Polinario, A. C.2; Silva, J. A.3; Freitas Neto, J. L.1; Leite, R. S.1; Santana, D. P.1

1 Universidade Federal de Pernambuco (UFPE). 2 Grupo de Pesquisa Sistemas de Liberação de Fármacos e Biofarmácia, Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). 3 Universidade Estadual da Paraíba (UEPB). Corresponding author: [email protected] Introduction: The technique of spray drying has been widely applied in obtaining dry extracts with better technology characteristics and greater concentration of constituents with biological activities important for the pharmaceutical area. Objectives: To characterize physico-chemically the dried extract of Opuntia ficus-indica (L.) Miller, using thermoanalytical techniques and infrared spectroscopy (IR). Materials and Methods: The hydroalcoholic extract of cladodes of Opuntia ficus-indica was used to obtain the nebulized extract in a Spray Dryer Lab Plant SD-05. The drying temperature was 160°C and used up 20% of aerosil®. This dried extract was thermally characterized by DSC, TG and DTA, using a sample of 2 mg and a heating rate of 10°C/min in thermoanalyticals equipments (Schimadzu). It also applies to the IV spectroscopy (Perkin Elmer Spectrum 400). Results and Discussion: The thermograms observed indicate that there is no defined stages of transition from matter (DSC), thermal degradation (TG) and physical transformations (DTA). This is due to the complexity of the sample, which is a plant extract. The IR spectrum showed absorptions in the region of 3200cm-1 and 1650cm-1 suggesting the presence respectively of hydroxyl and carbonyl. The strong absorption in the region of 1100cm-1 (ethers), suggests the presence of a large class of secondary metabolites, including flavonoids. Conclusions: The thermoanalytical techniques and IR spectroscopy allowed to characterize the dry extract of Opuntia ficus-indica, for subsequent use of this plant material in the pharmaceutical area. Financial support from Capes and CNPq



247

CODE 229 EXPRESSION STUDY OF THE GENES INVOLVED ON FOLIC ACID

METABOLISM IN ASSOCIATION WITH ORAL CLEFT DEVOLOPMENT

Soares, C. D.1; Oliveira, G. H. M.1; Cardoso, M. L.1; Bezerra, J. F.1; Souza, Santos, F. M1. K. S.1; Morais, L. V. F. 1; Oliveira; S. R.1; Farjado, C. M.2; Silbiger, V. N.1; Luchessi, A. D.2; Hirata, R. D. C. 2; Almeida, M. G1; Rezende, A. A.1; Hirata, M. H.2

1 Federal University of Rio Grande do Norte (UFRN). 2 Molecular Biology Laboratory, Toxical and Clinical Analisys Departament,

State University of Sao Paulo. Introduction: There is considerable evidence suggesting that folate-related genes play a role in the etiology of oral facial clefts. Clefts are known to have a strong genetic component. The expression profile of genes involved on the pathway and folic acid metabolism which is linked to oral clefts requires appropriate environmental causes in combination with genetic factors that culminate in the failure of fusion of facial processes. Objective: The aim of the present study was to analyze the gene expression profile of MTHFR, MTR, MTRR, and RFC1, in association with the occurrence of oral clefts. Materials and Methods: Were studied 80 subjects, 40 nonsyndromic oral cleft individuals, and 40 control subjects without oral cleft. Serum biochemical measurements were performed of folic acid, vitamin B12 and homocysteine. A questionnaire was given to mothers about the use of cigarettes, alcohol and family history. Total RNA was extracted from peripheral blood leukocytes. We perform the qPCR with applied inventoried gene expression assays. Results and Discussion: Individuals with oral cleft had reduced serum folic acid. The alcohol use was reported as significant by mothers of affected individuals. Syndromic oral cleft individuals had a significant reduction in the expression of mRNA for the genes of MTR, p = 0.013, and MTRR, p = 0.003. The same result was not observed in MTHFR and RFC1 genes, p = 0.143 and 0.490, respectively. Conclusions: The significant reduction in mRNA expression of enzymes MTR and MTRR in children with oral clefts associated with the reduction of folic acid and alcohol use by mothers, suggests that these patients have a genetic predisposition and the environmental factors influence on the appearance of malformations such as oral clefts. Financial support from FAPESP/Brazil and master scholarship from CNPq/Brazil.



248

CODE 230 PARAMETERS KINETICS BY OZAWA MODEL FOR NICOTINAMIDE BY

DYNAMIC TG Souza, F. C. de1; Silva, E. P. da1; Almeida, V. D. de1; Azevedo, W. M. de1; Aragão, C. F. Soares1; Gomes, A. P. B.1

1Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR), Federal University of Rio Grande do Norte (UFRN) Introduction: Thermal analysis methods are well-established techniques in research laboratories of pharmaceutical industry, and thermogravimetry (TG) has also been used to elucidate the kinetics of decomposition reactions. Arrhenius equation and model Ozawa have been used to calculate energy of activation and order of reaction from thermogravimetric and volumetric curves. Applies the method of Ozawa for dynamic TG curves and Arrhenius equation for the isotherms. Objective: Comparative study between the use of the method of Ozawa and Arrhenius equation for dynamic thermogravimetric curves of nicotinamide. Materials and Methods: Nicotinamide raw material and capsules were obtained from Farmafórmula Manipulation Pharmacy. It was weighted 10.5±0.5 mg in alumina crucible. For kinetic determination, dynamic TG curves were obtained using a Shimadzu thermobalance, model DTG–60, under a nitrogen flow at 50 mL.min–1 and heating rates of 10, 20, 40, 60 and 80°C.min–1 up to 900°C (n=3). The following kinetic parameters by Ozawa model kinetic energy, order and frequency factor. Results and Discussion: The nicotinamide showed the following kinetic parameters: order(0.0), kinetic energy (80.95 KJ/mol) and frequency factor(3.56 x 107 min-1) fo α – 0.1, as the nicotinamide capsules presented the following kinetic parameters: order(0.5), kinetic energy (63.93 KJ/mol) and frequency factor(2.93 x 106 min-1) fo α – 0.1. Conclusions: Nicotinamide capsules have a lower thermal stability than the raw material due to nicotinamide raw material has lower activation energy, which shows that there may be some interaction in the formulation. Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and FAPERN/Brazil.

http://oai.dtic.mil/oai/oai?verb=getRecord&metadataPrefix=html&identifier=AD0646562



249

CODE 231 DEVELOPMENT AND VALIDATION OF A STABILITY INDICATIVE

METHOD OF ASCORBIC ACID (VIT C) FOR 500 MG TABLETS Oliveira, A. R.2,4; Santana, A. K. M.1; Santos, B. A. dos1; Medeiros, F. P. M. de1; Silva, M. J. da1; Dias, M. E. de S.3 1 Laboratório Farmacêutico do Estado de Pernambuco Governador Miguel Arraes (LAFEPE). 2 Universidade Estadual da Paraíba (UEPB). 3 Universidade Federal de Pernambuco (UFPE). Corresponding author: [email protected] Introduction: Official compendia do not comprise a method for the concentration quantification of active pharmaceutical input (API) Vit C by HPLC, which allows evaluating related substances and potential synthesis impurities.Objective: Develop an indicative analytical methodology for stability study of ascorbic acid. Materials and Methods: The degradation studies were conducted with API, utilizing H2O and solutions of HCl 1,0M, NaOH 1,0M and H2O2 at 3,0%, at temperature (between 15 and 30ºC), protected from light, during 15 days. The sample-solutions were diluted with the degradation solution. At each analysis time, an aliquot was diluted to final concentration of 500 ppm, with mobile phase acetonitrile:ammonium acetate buffer. The developed quantification method by HPLC was validated to the API and co-validated to the Vit C 500 mg tablet according to the RE 899/2003 – ANVISA. Results and Discussion: When submitted to HCl the active degraded 99,3% in 15 days. With NaOH was of 93,16% in 24 hours of exposition. In H2O2 did not present a peak of the active at time zero, that is, it was degraded immediately. With H2O, the reduction after 15 days was of 46,73%. The utilized method was evaluated, showing to be linear, selective, precise, exact and robust. The method for the concentration quantification of Vit C in tablet was co-validated, thus presented linear, specific and precise. Conclusion: The forced degradation study indicated a new method to Vit C quantification by HPLC. The utilized method has been sensitive to detection of possible degradation products, it was selected as an indicative method of stability.



250

CODE 232 DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD

FOR QUANTIFICATION OF SIMVASTATIN IN MICROEMULSION BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Ferreira, L. F.1; Silveira, W. L. L.1; Xavier Júnior, F. H.1; Damasceno, B. P. G. L.2; Egito, E. S. T.1

1 Laboratório de Sistemas Dispersos, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). 2 Departamento de Farmácia, Universidade Estadual da Paraíba (UEPB). Corresponding author: [email protected]

Introduction: Unreliable analytical data can lead to incorrect decisions and irreparable financial damage. Validation is a method that ensures the specificity, accuracy and precision of an analytical test and thus ensures reliable and reproducible results of a sample. Objectives: To develop and validate an analytical method for quantification of simvastatin (SIV) in microemulsion (ME) by high performance liquid chromatography (HPLC), in accordance with the regulation 899/2003 of the National Sanitary Surveillance Agency. Materials and Methods: The HPLC column was a BDS Hypersil® C18, measuring 150 x 4.6 mm i.d and the mobile phase consisting of mixture acetonitrile and methanol (90:10, v/v), at a flow rate of 0.9 mL min-1. Analytes were measured by a UV detector set at 238 nm. The calibration curve was obtained from solutions of SIV in acetonitrile at predefined concentrations. The SIV was incorporated at a concentration of 10 mg.mL-1 into the ME. The samples SIV-ME were subsequently diluted in acetonitrile for the test assay. Results and Discussion: After the chromatografphic analysis, the results obtained for precision measurements (coefficient of variation) did not exceed 5%. The average percentage recovery was between 98% and 102%. The coefficient of regression linear (R2) and the values of limit of detection and quantitation obtained proved the linearity and sensitivity of the method, respectively. Conclusions: The results revealed that the method was specific, precise, accurate, robust and linear in the range from 4 to 18 µg.mL-1. Therefore, it can be safely used to quantitation of SIV in microemulsion. Financial Support from CNPq/Brazil.



251

CODE 232.2 RETROSPECTIVE AND CONCURRENT VALIDATION OF THE

MANUFACTURING PROCESS OF ZIDOVUDINE 300MG + LAMIVUDINE RETROSPECTIVE AND CONCURRENT VALIDATION OF THE MANUFACTURING PROCESS OF ZIDOVUDINE 300MG + LAMIVUDINE 150MG Oliveira, A. R.2,4; Magalhães, L. C. de3; Oliveira, A. D. P. de1; Malheiro, J. P. T.2; Monteiro, D. B.1; Granjeiro Jr, S1; Vasconcelos, F. V. C. de1; Araújo, E. S. de1 1 Laboratório Farmacêutico do Estado de Pernambuco Governador Miguel Arraes (LAFEPE). 2 Universidade Estadual da Paraíba (UEPB). 3 Universidade Estadual de Feira de Santana (UEFS). Corresponding author: [email protected] INTRODUCTION: In the pharmaceutical industry, validation is a practice directly related to Good Manufacturing Practices (GMP), and a component of quality assurance associated with a particular product or process. The Statistical Process Control is a tool whose main purpose optimize production, bringing higher quality products and cost reduction. OBJECTIVE: Validate the manufacturing process of Zidovudine (AZT) 300mg + Lamivudine (3TC) 150mg. MATERIALS AND METHODS: The study was conducted on the production line of the antiretroviral of the LAFEPE. First, the records using batch produced in 2010, were developed control letters and histograms, using the software WinCEP® plus 5, with data on weight, friability, hardness, disintegration, content and uniformity of dosage units. The second part consisted of a concurrent validation of all steps of the production process of thee lots, as is recommended in the RDC 17/2010 – ANVISA, and compared against the limits established in the retrospective validation. RESULTS AND DISCUSSION: The retrospective validation process characterized as capable in all parameters. The results presented for samples collected in the production process were mostly within the limits established in the letters controls, minor deviations in the uniformity and content of some samples, but not running away from the specifications laid down by law. CONCLUSIONS: The production process of AZT + 3TC met the requirements of GMP, and it can be said that the drug provides security in the final product. The statistical analysis of historical data proved to be important because the values were significantly validation alert, which diverted the limits, even meeting the specifications.



252

CODE 233 A STUDY OF CYTOTOXICITY BY BIOASSAY TECHNIQUES OF THE

HOMEOPATHIC PRODUCTS CONTAINING Momordica charantia Espósito, R. C.1; Andrade, M. G. M.1; Micali, I. A.3; Navarro, M. V. M.3; Rocha, L. G.2; Aragão, C. F. S.1

1 Quality Control of Drugs Laboratory, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN). 2 Laboratory of Antiparasitic Testing, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte (UFRN). 3 Laboratory of Homeopathic Pharmaceuticals, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN).

Corresponding author: [email protected] Introduction: The quality control of homeopathic preparations using common analytical techniques is challenging because its characteristic of high dilution. Biological assays in vitro have been shown positive results to evaluate the mechanism of action of homeopathics, able to act on a specific system such as homeopathic equalizers (BELLAVITE et al 2006). Objective: This study aims evaluated the quality of homeopathic products containing Momordica charantia leaf and fruit of 3DH and 6CH with biological assay in vitro. Materials and Methods: The cytotoxic activity of homeopathic products containing Momordica charantia leaf and fruit of 3DH and 6CH potencies have been dinamized with milli-q water and their mother tinctures (TM) according to the Brazilian Homeopathic Pharmacopoeia (FHB) against procyclic promastigotes cells of Leishmania (L) chagasi. The samples (n=5) were prepared and incubated for 24 hours in a Qualxtron biological camera at 27°C and the viable cells was quantified in a Neubauer chamber through the optical microscope. Results and Discussion: Mother tinctures showed cytotoxicity to cells during incubation and TM leaf showed higher cytotoxic activity than homeopathic products. The dynamized medicines 6CH showed more cytotoxicity than 3DH. Conclusions: These results indicate that homeopathy products containing Momordica charantia have cytotoxic properties. Financial support from CAPES/Brazil and PROPESQ-REUNI-UFRN/Brazil.



253

CODE 234 UV-VIS ANALYSIS OF CHEMICAL MARKERS OF THE LEAVES OF

Passiflora edulis Sims VARIETY edulis Soares, T. C¹; Siqueira, E. M. S.1; Reginatto, F. H.²; Ramos, F. A.³; Castellanos, L.³; Duque, C.³; Schenkel, E. P.²; Zucolotto, S. M.1

1Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). ²Universidade Federal de Santa Catarina (UFSC), Florianópolis-SC, Brasil. ³Departamento de Química, Universidad Nacional de Colombia (UNAL). Introduction: Brazil has a large variety of species of Passiflora genus. Known as maracujás, the species Passiflora edulis variety flavicarpa Degener (yellow passion fruit/maracujá-amarelo) and Passiflora edulis Sims variety edulis (purple passion fruit/maracujá-roxo) occur widely in the South America and are popularly used in the treatment of anxiety, migraine, nervousness and insomnia. The glycosilated flavonoids are the main compounds related of these species. Objective: The aim of this study was to isolate the main compounds of the leaf extract from Passiflora edulis Sims variety edulis to differentiate the varieties of P.edulis. Materials and Methods: The leaves of P. edulis Sims variety edulis were collected in Colombians Andes. The aqueous extract of leaves was made by infusion and partitioned with ethyl acetate (EtOAc) and n-butanol (BuOH). The thin layer chromatography (TLC) analysis was performed using different systems, NP reagent A as detector and observation on UV 254/365 nm. The BuOH fraction was submitted to many chromatographic processes as flash column, chromatographic column using silica gel, and Sephadex LH 20. These processes resulted in a purified fraction, that was submitted to UV analysis. Results and discussion: The compound showed a Rf 0.85 and a spot deep purple (UV 254 nm), but no developing color after revelated with NP reagent A. In the UV spectrum was observed UV maximum absorption at 261 nm (band II) and lacks the band I, characteristic in the flavonoids Conclusion: These results showed that the comparison between UV data of the literature and the characteristics observed by TLC suggested that the compound present similarity UV absorption of a chrysin.



254

CODE 236 ANTICONVULSIVANT AND ANXIOLITIC ACTION EVALUATION OF

INDIGOFERA SUFFRUTICOSA MILLS AND INDIGO CARMINE IN MICE. Soares, P. H. S.1; Almeida, E. R.2; Chaves, T. M.3; Silva, A. R.2; Aragão-Neto, A. C.2; Couto, G. B. L.2; Luna, R. L. A.2, Silva, R. P.2

1Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco (UFPE). 2Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE). 3Departamento de Odontologia, Universidade Federal de Pernambuco (UFPE). Corresponding author: [email protected]

Introduction: The use of plants in he c ed “fo k med c ne” s characteristic of Brazilian cultural heritage. Among the numerous plants popularly used Indigofera suffruticosa M s found. Known s “ n e ” nd indigo field, is used in the treatment of renal and prostate diseases. Objective: The present study had as an objective the evaluation of the anticonvulsant and anxiolytic action of Indigofera suffruticosa Mill and indigo carmine molecule. Materials and Methods: Swiss albino mice (Mus musculus) aged between 45-60 days and weighing 22-32 g were used. The mice had been submitted to doses of 100, 300 and 500mg/kg of the methanol extract and 30 mg/kg of indigo carmine dissolved in saline solution. To anticonvulsivant action evaluation, after one hour, the groups of animals had suffered the reaction from following convulsivants,: Pentylenetetrazole, Picrotoxin, Strycnine, Pilocarpine and Lidocaine. To anxiolytic study, the mice had been submitted to the Plus-Maze and Marble-Burying tests. As standard drug, Diazepam was used in all the experimental models. Results and Discussion: The methanol extract presented excellent anticonvulsant action, increasing the time of latency for the beginning of the first convulsion in all the tests, an effect similar to that presented by the indigo carmine. Conclusions: The results of this study demonstrate that this is a promising plant for future studies in the development of new anticonvulsants drugs, and indigo carmine as an isolated molecule.



255

CODE 237 DEVELOPMENT OF NEW METHODS OF ANALYSIS AND CONTROL

OF METRONIDAZOLE TABLETS USING NEAR INFRARED AZEVEDO, L. F. 1*; Dias, S. B. T.1; Rocha, V. N.2; França, D. M. M.2; Brito, K. G. S. 1; Silva, E. L. 1; Nascimento, T. G. 1 1Laboratório de Análises e Controle de qualidade de Fármacos e Medicamentos, Grupo de Pesquisa em Tecnologia e Controle de Qualidade de Medicamentos,Curso de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas(ESENFAR/UFAL) – Av. Lourival Melo Motta, s/n, Campus A.C. Simões, Tabuleiro dos Martins, Maceió – Alagoas- CEP: 57072-970. 2Laboratório Industrial Farmacêutico do Estado de Alagoas, Setor de Control de Qualidade de Medicamentos(LIFAL) Corresponding author: [email protected] Introduction: The development of new methods of quality control with low-cost and reproducible for drugs, bulks and medicines are essential to establish new criteria and quality specifications. Assays of medicines using near-infrared (NIR) can be a viable alternative in the development stage of new products LIFAL. Objective: The work aimed to evaluate the compatibility of metronidazole and excipients used in the manufacture of tablets and identify possible excipients used in a standardized composition of metronidazole using the near infrared technique. Materials and Methods: The compatibility study was conducted based on the analysis of binary mixtures, which consisting in physical mixtures of metronidazole (active ingredient) and pharmaceutical excipients (lactose, cellulose MC 101, talc, magnesium stearate and aerosil) in pre-determined proportions. The raw materials, binary mixtures and standardized tablets of metronidazole were subjected to analysis of near-infrared. Results and Discussion: Using NIR analysis, it observed that binary mixtures metronidazole-excipients do not present pharmacotechnical incompatibilities, because the NIR bands of the raw material of metronidazole are maintained. Comparison of infrared spectrum of binary mixtures and the standardized tablet of metronidazole revealed the appearance of stretch at 4750cm-1, 5150 cm-1and 6850 cm-1 which are derived from cellulose MC 101 without possibility of these bands, mainly (5150 cm-1), to occur in the lactose excipient. Conclusion: The infrared spectrum was very useful to analyze the compatibility of excipients in formulations of metronidazole tablets, besides to provide technological information to detect the presence of specific composition in standardized formulation of metronidazole. Financial support from CNPq/Brazil.




256

CODE 238.1 CARACTERIZATION OF RIFAMPICIN AND THEIR DEGRADATION

PRODUCTS BY CHEMICAL STRESS Amorim, M.V.P.¹; Assis, W.R.S¹; Costa, A. F. O¹; Araújo, A. A²; Aragão, C.F.S.¹ ¹Laboratório de Controle de Qualidade, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). ²Laboratório de Farmacologia, Departamento de Farmacologia, Universidade Federal do Rio Grande do Norte (UFRN). e-mail: [email protected] Introduction: The drugs have been more and more researched. According to ANVISA, the procedures have to present identification and quantification results of degradation products, followed by analytical method correspondent. For this, the procedures have to present initial studies using the drugs in some stress conditions. Rifampicin is one of the main drugs chose to treat the tuberculosis, an illness considered negligible in many countries. Objective: The objective was to characterize the Rifampicin and its degradation products after a chemical stress. Materials and Methods: A chemical stress was carried out using 0.1N HCl and 0.1N NaCl. Furthermore, they were analyzed using a C8 (125mm x 4.6mm) column at a flow rate of 1.6 mL.min-1, with a run time of 15 min at 254nm. The mobile phase was a mixture of phosphate buffer, water, acetonitrile, 1.0 M citric acid and 0.5 M sodium perchlorate (100:350:510:20:20 v/v). Results and Discussion:Two samples were prepared consisting of Rifampicin and their degradation products, separately, containing about 0.2 mg of each per mL in a mixture of water and acetonitrile (50:50 v/v). Injections of equal volumes (50 µL) of both solutions were inserted at the chromatograph and the peaks could be compared. Both chemical stress (acid and basic) were successful, due a complete degradation, once compared to the solution containing Rifampicin. Also, the visual aspect of the both degradation products solutions and the Rifampicin were different. Conclusion:Further studies will be conducted in order to carry out others stress tests, as photolytic, heat, moisture and oxidative. Financial Support from Master scholarship from CAPES/Brazil.




257

CODE 238.2 COMPARISON STUDY OF TWO ANALYTICALS METHODS OF 4-FDC

USING A SINGLE COLUMN Amorim, M.V.P.¹; Costa, A. F. O¹; Araújo, A. A²; Aragão, C.F.S.¹ ¹Laboratório de Controle de Qualidade, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN). ²Laboratório de Farmacologia, Departamento de Farmacologia, Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author: [email protected] Introduction: Tuberculosis is one of the illnesses most dangerous, but can be curable in practically 100% of new cases, since the principles of modern chemotherapy are obeyed. The drugs considered first class in the treatment are: Isoniazid, Rifampicin, Pirazinamide and Ethambutol (4-Fixed Dose Combination or FDC). Objective: The aim is a comparison of two screenings involving acetonitrile and methanol to obtain the 4-FDC in a single column. Materials and Methods:Two screenings were done with a C18 (250 mm x 4.6 mm) column at a flow rate of 1,5 mL.min-1 with a run time of 60 min and DAD range 200 – 800 nm. The buffer solution (ammonium acetate and copper II acetate monohydrate in water) was used on the mobile phase with acetonitrile in the first screening and with methanol in the second. A sample containing the 4 drugs and 4 samples containing the drugs separately were prepared into volumetric flasks. Both screenings obtained the 4 drugs into the same analyze. Results and Discussion: The retention time with acetonitrile presented 7.34, 7.91, 10.59 and 32.30 min, while methanol 8.11, 8.98, 14.33, 43.86 min of Isoniazid, Pirazinamide, Ethambutol and Rifampicin, respectively. However, the screening with acetonitrile showed system suitability (tailing factors, theoretical plates, resolutions and widths) better than that consisted of methanol. Many wavelengths were analyzed and the best was 270 nm. Conclusion: Further studies will be conducted in order to improve this analytical method. Financial Support from Master scholarship from CAPES/Brazil.




258

CODE 239 EVALUATION OF ANALYTICAL METHODS APPLIED TO

QUANTIFICATION OF LIPID PEROXIDATION IN LIVER HOMOGENATE OF RATS Morais, L.1, Araújo-Silva, G.1, Solon, L.G.S.2, Almeida, MG.1

1LABMULT, Department of Clinical and Toxicological Analyses - UFRN/Natal-RN/Brazil 2Quality Control of Drugs Laboratory, Department of Pharmacy- UFRN/Natal-RN/Brazil;

Corresponding author: [email protected] Introduction: The lipid peroxidation (LP) is one of the main results of oxidative stress. It occurs in polyunsaturated fat acids, starting with the d c •OH, leading to formation of alcooxil and peroxyl radicals. The malondialdehyde (MDA), which is the most abundant product in LP, induces structural modifications in biological molecules. This aldehyde is the main marker of LP. It is commonly evaluated by spectrophotometry through reaction with Thiobarbituric Acid (TBA) thus due to numerous interfering it is evaluate as reactive substances of this chromogenic reagent (TBARS). Objective: The purpose of this work was to set a comparison between analytical methods enshrined in the scientific literature about determination of lipid peroxidation marks in liver homogenate of rats (control and intoxicated groups) by CCl4. Materials and Methods: At first was used reaction TBARs, N-metil-2-fenilindol (MDA-MFL) reaction, and quantificacion of complex TBA-MDA by HPLC-DAD. Results: In control animals group the parameters of lipid peroxidation (TBARS = 23,72 ± 1,4 µmol/L ; MDA-MFL = 19,63 ± 2,6 µmol/L) were decreased in comparison with intoxicated animals by CCl4 (TBARS = 78,83 ± 12,4 µmol/L ; MDA-MFL = 65,54 ± 8,6 µmol/L ). The results MFL-MDA were lower than TBARS in the same group, suggesting absence of interfering. Conclusion: The comparison of TBARS and MDA-MFL methods showed that the second one was more specific than the first one. Other results will be obtained by HPLC-DAD analysis, also for comparison, due its specific methodology for quantification of MDA. Finnantial supported from CNPq/Brazil.



259

CODE 240 VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR

DETERMINATION OF PROPRANOLOL IN CAPSULES USING QUITOSAN AS EXCIPIENT Fonseca, G. D.1,*; Medeiros, A. S. A.1; Fernandes-Pedrosa, M. F. 1; Silva Junior, A. A. 1; Santos, K. S. C. R.1 1Laboratório de Tecnologia e Biotecnologia Farmacêutica (TecBioFar), Departamento de Farmácia (DFAR),Universidade Federal do Rio Grande do Norte (UFRN). Corresponding author *[email protected] Introduction: Chitosan is a linear polysaccharide composed by copolymers of glucosamine and N-acetyl glucosamine obtained from partial deacetylation of chitin. Objective: To study chitosan as excipient in solid dosage forms, propranolol was used as probe drug to evaluate the chitosan performance as diluent and disintegrant in capsules. Materials and Methods: A UV spectrophotometric method for determination of propranolol in capsules containing quitosan was validated. The parameters specificity, linearity, precision and accuracy were studied according to validation guidelines. Results and Discussion: The method showed specificity and linearity in the concentration range of 32 to 48 mg.mL-1, presenting a good correlation coefficient (r = 0,9994). The intra and interday precision data demonstrated the method has good reproducibility (relative standard deviation < 2,0 %). The accuracy determined from recovery test presented satisfactory recoveries percentual of propranolol (103,4 - 109,4 %), which indicates there was no interference of the excipients in the determination of the active pharmaceutical ingredient. Conclusions:These results show that the method can be applied to assay propranolol in capsules using chitosan as excipient. Financial support from CNPq and FAPERN/Brazil



260

CODE 241 CHROMATOGRAPHIC PROFILE OF THE CRUDE EXTRACT AND

FRACTIONS FROM K. brasiliensis Cambess (SAIÃO) LEAVES Fernandes, J. M.¹; Negreiros Neto, T. C.¹; Oliveira, A. C.¹; Araújo-Silva, G.²; Morais, L. V. F.²; Soares, L. A. L³; Zucolotto, S. M.¹ ¹Laboratório de Farmacognosia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil. ²LABMULT, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil. ³Departamento de Farmácia, Universidade Federal de Pernambuco, Recife, PE, Brasil. Corresponding author: [email protected] Introduction: The specie Kalanchoe brasiliensis Cambess, (Crassulaceae), known popularly as saião, is widely used in folk medicine as anti-inflammatory and healing. There are not works about the chromatographic profile of the chemical markers of this species. Objectives: The aim of this study was to evaluate the chromatographic profile of the crude extract and fractions from the leaves of K. brasiliensis by High Performance Liquid Chromatography (HPLC) with Diode Array Detector (DAD) to characterize the chemical markers of this species. Materials and Methods: The hydroethanolic extract (HE) 50% was obtained by turboextraction from fresh leaves. The HE was submitted a partition, resulting in the CHCl2 and AcOEt fractions. The HPLC analysis was performed using a Lichrospher RP 18-5 column, in a gradient system- water:formic acid (99.7:0.3, v/v) and MeOH:formic acid (99.7:0.3, v/v), flow (0.8 mL/min) and UV detector (254 and 370 nm). Results and Discussion: The chromatogram of the HE showed six main peaks. All peaks had similar UV spectrum with three maximum absorption (259 nm and 271 nm- band II and 355 nm- band I), characteristic of the flavonoids nucleous. The chromatogram obtained of the AcOEt showed six main peaks, with had similar UV spectrum with two maximum absorption at 262 nm (band II) and 358 nm (band I). The chromatogram of CH2Cl2 fraction showed five main peaks with two different spectrum profiles (1. 262 nm- band II and 358 nm- band I; 2. 232 and 268 nm- band II and 324 and 362 nm- band I). According with literature data, suggests that the main compounds of the HE are patuletin-3-O-glycoside derivatives. Conclusions: This method showed a chromatographic profile satisfactory, with good resolution and symmetrical peaks. The fraction AcOEt fraction showed the main compounds of the HE. Further studies are been conducted to characterize these patuletin-3-O-glycoside derivatives.



261

CODE 242.1 KINETCS STUDIES OF Euterpe oleracea (AÇAÍ) EXTRACTS

Daher, C. C. 1; Rodrigues, R. O.2; Fontes, I. S. 2; Soares, D. S. 2; Ferrari, M.2; Aragão, C. F. S.3. Gomes, A. P. B. 3

1 Faculty of Pharmacy - Potiguar University 2 Laboratory of Research and Development of Cosmetic Products - Faculty of Pharmacy, Federal University of Rio Grande do Norte. 3 Laboratory of Quality Control – Faculty of Pharmacy, Federal University of Rio Grande do Norte. Corresponding author: [email protected] Introduction: Euterpe oleracea is an Amazon palm tree. Their extract can be used in cosmetics preparations. The thermals methods are used to characterize actives and excipients and kinetics studies. Objectives: The aim of this work was to evaluate and compare the kinetics behavior of glycolic extract and dried extract as raw materials. Materials and Methods: The TG curves were obtained with a Shimadzu thermobalance (DTG-60), under a N2 flow 50mL.min-1, at heating rate 10oC.min-1, up to temperature of 900ºC. Sample mass about 10mg were packed in an alumina cell. For the decomposition, the activation energy (Ea), the order of reaction (n) and the frequency factor (Z) were obtained from first step of TG curves. The curves obtained were analyzed for software Shimadzu

TA60 TASYS. The kinetic parameters were calculated with values of 0.1–0.9 by utilizing the mathematical equations of Coats-Redfern (CR), Madhusudanan (MD), Horowitz-Metzger (HM), and Van-Krevelen (VK). The kinetic data were calculated by four mathematics models. Results and Discussion: Presents the values of the order of reaction (n), the activation energy (Ea) and the frequency factor (Z) for glycolic extract and dried extract, with the best linear correlation coefficients (r). Glycolic extract (n / Ea - kJ.mol-1 / Z/s-1): CR (1.57/56.98/4.29x106); MD (1.61/57.93/6.77x106); HM (1.91/71.77/1.03x109); VK (1.79/ 64.73/9.48x1012); Dried extract: CR (4.82/73.73/6.52x109); MD (4.62/72.15/3.64x109); HM (4.88/99.26/6.04x1012); VK (4.80/85.95/7.31x1016). Conclusions: It was found that the integral methods (CR and MD) and approximation methods (HM and VK) yielded kinetic data that were reasonably comparable with each other. Financial support from CNPq/Brazil and undergraduate scholarship from UFRN/Brazil.



262

CODE 242.2 SUNSCREENS O/W EMULSIONS STABILIZED BY RHEOLOGICAL

MODIFIERS: PRELIMINARY STABILITY TESTS Daher, C. C. 1; Fontes, I. S. 2; Rodrigues, R. O. 2; Soares, D. S. 2; Ferrari, M.2. 1 Faculty of Pharmacy - Potiguar University 2 Laboratory of Research and Development of Cosmetic Products - Faculty of Pharmacy, Federal University of Rio Grande do Norte. Corresponding author: [email protected] Introduction: The emulsions are systems thermodynamically instable. The effect of some additives to increase the viscosity can be used to improve the stability. Objective: The aims of this work were to development and evaluate the preliminary stability of sunscreens formulations containing rheological modifier. Materials and Methods: Emulsions were prepared with Acrylates/C10-30 Alkyl Acrylates Crosspolymer (AAAC) (0.3%). To improve the stability the different concentration of Polyacrylamide and C13-14 Isoparaffin and Laureth 7 (PCIL) (0.5% PS1, 1.0% PS2 and 1.5% PS3) were used. The centrifugation test, freezing-thawing cycle (FTC) and thermal stress (TS) were used to investigative the preliminary stability tests. The formulations were assessed through stability was assessed through the organoleptic characteristics, pH and electrical conductivity values, macroscopic analysis and rheological behavior determinations. Statistical analyses were performed (p<0.05). Results and Discussion: The O/W emulsions were prepared. The samples did not show any changes in aspect after the centrifugation test. The PS1 was unstable in the preliminary stability tests. The PS3 formulation showed statistical differences in the electrical conductivity values after TS and FTC tests. An improvement in the stability was shown when the emulsion was formulated with PCIL (1.0% - PS2). Rheological measurements showed that these emulsions exhibited non-Newtonian pseudoplastic flow behavior. Conclusions: O/W emulsions containing 0.3% of AAAC and PCIL (1.0%) have been found to be preliminary stables and should be conducted to accelerated stability tests. The authors thank Basf, Chemyunion, Croda, ISP and Mapric for the gift of sample materials. Financial support from CNPq/Brazil and undergraduate scholarship from UFRN/Brazil.



263

CODE 243 STUDY BY QUANTUM MECHANICAL OF INTERACTIONS BETWEEN

AMPA RECEPTORS AND FLUORINE WILLARDIINE Lima Neto, J. X.1, Ourique, G. S. 2, Oliveira, J. I. N. 1, Albuquerque, E.L. 1, Freire, V. N.3, Fulco, U. L. 1 1Department of Biophysics e Pharmacology (DBF), Federal University of Rio Grande North (UFRN). 2Department of Biochemistry (DBQ), Federal University of Rio Grande North (UFRN). 3 Department of Physics, Federal University of Ceará (UFC). Corresponding author: [email protected] Introduction: AMPA receptors are essentials in the development and homeostasis of in the nervous system, the deficiencies in normal functions of that kind of receptors are the major causes of damages in CNS. However, the responsible mechanism for this function aren't completely well knowledge. Objective: To elucidate this question, four willardiine partials agonists were obtained with fluorine willardiine (FW) if the major efficiency. Recent researches have indicated the QM methods like an effective way in the achievement of the receptor-ligand interaction values. In this work, we aim, the achievement of the AMPA-FW interaction energy, with the major importance residues. Materials and Methods: Using the crystallographic dates and the QM techniques named Molecular Fractionation with Conjugate Caps (MFCC), based in the proteic system fractionation, in which all of this amino acids fragment passed by quantum treatment. Results and Discussion: By the complexed crystallographic structure of the GluA2-FW we identified the amino acids Glu705 and Arg485 with the larger attraction (-68 kcal/mol and -50 kcal/mol) and Lys656 and Lys449 (10 and 12 kcal/mol) with the major repulsion. The residues Thr655, Leu650, Thr480, Leu479 were showed as important residues responsible for the maintenance of the receptor functions, the total energy of the system is -330 kcal/mol. Conclusions: With the obtainment of this dates, is possible a detailed understanding of AMPA and the creation of the new drugs against neurodegenerative diseases. Financial support from CNPq/Brazil




264

CODE 244

EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL ETHANOLIC EXTRACT FROM VERNONIA POLYANTHES Temponi, V.S.1; Silva, J. B.1; Fernandes, F. V.1; Alves, G. A. D1; Matos, D. M.1; Gasparetto, C. M.1; Pinho, J. J. R. G., Ribeiro, A.2; Alves, M. S.1; Sousa, O. V.1

1Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora 2Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora Corresponding author: [email protected] Introduction: Vernonia polyanthes, found in South America, is traditionally used as anti-rheumatic, healing and anti-inflammatory. Objective: The aim of this study was to investigate the anti-inflammatory activity of ethanolic extract from V. polyanthes. Materials and Methods: Dried and powdered leaves were extracted with ethanol by static maceration. The anti-inflammatory activity was assessed by paw edema and pleurisy methods. Statistical significance was determined by analysis of variance and Student Newman-Keuls test (p < 0.05). Results and Discussion: 1 and 2 h after the application of carrageenan, the paw edema was reduced by 19.05 and 22.03% at a dose of 400 mg/kg, respectively. The inhibition of edema was observed 3 and 4 h after carrageenan injection at the doses of 200 mg/kg (16.41 and 27.87% respectively) and 400 mg/kg (25.78 and 31.97%). The volume of exudate was reduced at the doses of 200 and 400 mg/kg in 13.83 and 43.08%, respectively. The total number of leukocytes was also inhibited at the doses of 200 (17.87%) and 400 mg/kg (28.39%). Based on these results, the anti-inflammatory effect of ethanol extract may be mediated by inhibition of prostaglandin synthesis. Conclusion: the data suggest that V. polyanthes can be an active source of substances with anti-inflammatory activity, which justifies the popular use. Financial support from CAPES/Brazil, FAPEMIG/Brazil and UFJF/Brazil.



265

CODE 245 EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL

ETHANOLIC EXTRACT FROM Vernonia condensata Silva, J. B.1; Temponi, V. S.1; Fernandes, F. V.1; Alves, G. A. D1; Matos, D. M.1; Gasparetto, C. M.1; Pinho, J. J. R. G.1; Ribeiro, A.2; Alves, M. S.1; Sousa, O. V.1 1Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora 2Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora Corresponding author: [email protected] Introduction: Vernonia condensata has been used in folk medicine as analgesic agent. Objective: The present study investigated the anti-inflammatory activity of ethanolic extract from V. condensata. Materials and Methods: Dried and powdered leaves were extracted with ethanol by macerating static. The anti-inflammatory activity was assessed by paw edema and pleurisy in rats. Statistical significance was determined by analysis of variance and Student Newman-Keuls test (p < 0.05). Results and Discussion: The inhibition of edema was observed from 2 hours after

administration of carrageenan at doses of 200 and 400 mg/kg (0.46 0.04

and 0.45 0.07, respectively). 3 hours after injection, the doses of 100, 200 and 400 mg/kg reduced paw edema in 21.50, 22.58 and 32.26 %,

respectively. Doses of 100, 200 and 400 mg/kg (0.85 0.05, 0.70 0.09

and 0, 74 0.06) after 4 hours of application also produced inhibition of edema in 22.72, 36.36 and 32.72%, respectively. The effects on pleurisy showed that the doses of 200 and 400 mg/kg reduced the volume of exudate (17.30 and 35.13%, respectively) and the inhibition of leukocyte

migration occurred at doses of 100, 200 and 400 mg/kg (10.18 0.24, 9.47

0.10 and 8.17 0.14, respectively). Based on these results, the anti-inflammatory effect of ethanol extract from V. condensata leaves may be mediated by inhibition of prostaglandin synthesis. Conclusion: this study showed that the crude ethanolic extract of the plant has anti-inflammatory activity, which justifies the popular use. Financial support from CAPES/Brazil, FAPEMIG/Brazil and UFJF/Brazil.



266

CODE 245 EVALUATION OF MUTAGENICITY AND ACUTE TOXICITY IN MICE OF

Spondias purpurea (Siriguela) ETHANOLIC EXTRACT.

Dantas, AM1; Coutinho, AC1; Luz, SMD1; Lima, MS1; Lira, WM2; Santos,

VL1*

1Laboratory of Pharmacological Assays, Department of Pharmacy, State University of Paraiba (UEPB), 2Laboratory of Genetics, Department of Biological Sciences, State University of Paraiba (UEPB). Corresponding author: [email protected] Introduction: The Spondias species, belonging to Anacardiaceae family,

is the second most important gender in Brazil and many species are

scattered throughout the country, some with wide occurrence in the

Northeast. Many of these species are used as medicines. The species

Spondias purpurea, known as Siriguela, is employed against renal pain,

such as antidiarrheal, antispasmodic, diuretic, analgesic and antianemic.

Objective: Evaluate the acute toxicity of S. purpurea ethanolic extrat and

its potential mutagenic effects using the standard micronucleus. Materials

and Methods: To determine the toxicity were used adult mice of both

sexes who received through the oral route 2000 mg/kg of the extract and

they were observed after 30 min., 1, 2, 3 and 4 hours daily until 14 days. In

the model for mutagenicity evaluation of the extract was administered at

doses: 500, 1000 and 2000 mg / kg orally. 24 hours after treatment blood

was taken from tails of mice. Results and Discussion: Acute

administration of S. purpurea extract (2000mg/Kg) did not promote

behavioral changes, mortality, and no difference in body weight gain and

vital organs and no mutagenic effect was found in the micronucleus test in

peripheral blood cells of mice. The micronucleus test evaluates in vivo

chromosome damage in rodent hematopoietic cells. Conclusion: The

ethanolic extract, at the doses used, did not show acute toxicity nor

promote change or genetic damage or damage to the DNA repair

mechanism which would result in an altered cell.

Financial support from CNPq/UEPB/Brazil


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