An Approach to the Mystery of WaterBased on Grander® Technology

An Interim Report by Dr. Horst Felsch

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Published by: Uranus Verlagsges.m.b.H., Lange Gasse 48/5, A-1080 ViennaTel.: 0043(0)1 403 91 11, fax: 0043(0)1 403 91 11 33, e-mail:uranus@uranus.at

Responsible for the contents: Dr. Horst Felsch, Academically Qualified Engineer, Schlossberg 25, A-6391 FieberbrunnTel.: 0043(0)5354 56 050, fax: 0043(0)5354 52 248, e-mail:h.felsch@tirol.com

Production: message Medien & VerlagsGmbH, Diefenbachgasse 5, A-1150 ViennaLitho: Brüll & Schor, Pröllgasse 4, A-1130 Vienna

Printing: Druckerei Berger, A-3580 HornCopyright: All articles ©2000, Uranus Verlagsges.m.b.H.

May be reprinted only with express permission

Microbiological Effects of Grander® Technology

Pin Point Formssation

Biological Method for Proving the Positive Transformation of Heavy Metal Information in Water, Using Grander® Revitalisation

The Phosphorescent Bacteria Test

Grander® Technology Improves the

The Preservation Qualities of Drinking Water

How Long Is Original Grander® Revitalisation Equipment Effective?

The Active Life-Span of Grander® Revitalisers

Contents

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Preface

“Water is a cosmic thing!” That was one of the things Johann Grander pressed upon me in 1993, when he entrusted me with the task of testing his discovery with scientifically recognised methods. This responsibility was, for me, the beginning of a very exciting per-iod and for the first time I had the feeling that I was drawing closer to the phenom-enon of “water and life”. On the other hand, I was very sceptical, because the relationship between “water and the cosmos” meant little to me.

The work got underway with a series of laboratory tests, with the involvement of Austrian university institutes. As a matter of principal, the practical implementation of Grander®

Technology in the industrial field was undertaken only after water analysis had clearly defined the initial conditions. After the equipment was installed, chemical and bacteriological tests documented any changes. This resulted in an abundance of data, which demonstrated the following: the use of Grander®

Technology results in a lower demand for chemicals, the bac-teriological quality of the water improves, and drinking water is preserved for years.

So much for the technical applications. But what about the scientific side of things?

Occasionally, during discussions I was confronted with the argument: Grander® Technology may well be effective, but no one really understands the principal of how it works, and ulti-mately the whole matter simply has to be accepted on faith.

In order to invalidate this notion that “faith” is required and also to give the users trust and confidence in Grander® Technology, the results presented in this brochure have been obtained using scientifically recognised test methods. Thus they can be checked by any properly equipped laboratory .

In this regard, I would like to make the following important point: the studies described here were written primarily for the “interested layman” rather than for the scientist. Despite the complicated context it was intended to keep the text universally comprehensible. The “complete version” of these studies will be published next year in book form.

And the cosmic thing? Here as well, we have made con-siderable progress. In 1997, a research contract related to Grander® Technology was awarded to the vice president of the Russian Academy of Natural Sciences, Prof. Yuri Rachmanin.

In March 2000, Professor Rachmanin and his colleague Profes-sor Kondratov came to Austria in order to report personally on their findings at Jochberg in Tirol. And there it was again: the connection between water and the cosmos. The scientists confirmed that the structure of water undergoes lasting change during Grander® revitalisation. They supplied proof that there is vertical compression of the hexagonal units. Thus water that has undergone Grander® revitalisation has qualities that are comparable with those of “oscillating quartz”: the vertical structures act as antennas that pick up the special frequencies of cosmic energy and transfer it (like a transmitter) to water that has not been revitalised.

So Johann Grander is right: water is connected with the cosmos.

To me, it has been very interesting to observe the uncompli-cated relationship that so-called “natural researchers” such as Viktor Schauberger and Johann Grander have to the phenome-non of water. They succeed in discovering relationships that are initially incomprehensible to “qualified” scientists. This is also the reason why I want to dedicate the present work to Johann Grander. With his highly descriptive language he has helped me find an entirely new relationship to natural phenomena. Thus the investigation of water as a “cosmic thing” is a matter that will continue to fascinate me for a long time to come.

Dr. Horst Felsch, Academically Qualified EngineerApril 2000

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T he expression “pin points” is taken from practical life and refers to the size of the bacteria. It is applied to bacterial colonies that are particularly small,

and the size of a pin-head is a well-chosen comparison.

Until only a few years ago the expression “pin points” was relatively little known in water research. Pin points were described in a dissertation submitted in 1972 by an Iranian student, Khodaia Mohaled. The title of his dissertation was Oligocarbophile: Micro-organisms in Plus Lake. The literal transla-tion of the name oligocarbophile bacteria implies that they like only a small amount of carbon; thus they are typical water bacteria, which have adapted to the very low levels of nutrients found in water. In flowing waters (i.e. streams) it is quite usual to find pin points within the spectrum of the living colonies of bacteria. This is seldom the case in drinking water.

In the meantime, the expression “pin points” has been admitted to the textbooks on microbiology, such as Hans G. Schlegel’s General Microbiology, published by Georg Thieme

Verlag in Stuttgart, Germany. It contains an illustration that shows the difference in size between “wild” colonies and pin point colonies. In the present report, I would like to use bacteriological methods to demonstrate that the massive appearance of pin points provides clear evidence of Grander® revitalisation.

A page from the textbook General Microbiology, in which the expression

pin points is described. In the illustration one can see large white colonies

of the “wild” type – which is the original bacterium. The small white dots

are pin points. The difference in size is quite apparent.

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Microbiological Effects of Grander® Technology

Pin Point FormationFirst investigations have proved that Grander® Technology changes the cluster structure

of water. Above all, this should effect bacteria, which have maintained a living relationship (biocoenosis) with water from time immemorial. Changes in the

cluster structure should of necessity also lead to bacteriological changes. This was the basic premise for the investigations that are described below.

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The Ability of Water to Purify Itself

Polluted water has usually lost many of the qualities that unpolluted water still possesses, e.g. the ability to assimilate sufficient quantities of oxygen. Other qualities include freshness, taste and smell. Polluted water is often no longer capable of purifying itself. The bacteria in polluted waters are unable to break down the large quantity of nu-trients found in these waters. Usually the necessary oxygen is lacking, so that anaerobic processes are more likely to take place, creating unpleasant smells. Thus our wastewater treatment plants need huge quantities of oxygen from the air in order to stimulate micro-organisms in the “revitalisa-tion pool” to reduce the levels of pollution.

How Is the Bacterial Count of Water Determined?

For the determination of the bacterial count, a method is used in which the bacteria are cultured on an artificial culture medium. Individual bacteria create larger colonies that can be seen with the naked eye and can thus be counted without the use of a microscope. The method for determin-ing the bacterial count in drinking water has been standard-ised along with the composition of the culture media to be used. In principal there are two methods for determining the bacterial count: the plate culture method and the mem-brane filter method. All the investigations carried out by me were conducted using the membrane filter method. Thus I would like to explain the method in greater detail.

In determining the bacterial count, 1 ml of the drink-ing water being tested (or 1 ml of an appropriate dilution) is

Robert Koch in the year 1886. He was the first to succeed in growing

bacteria on an “artificial” culture medium. He used meat soup as a medium

in the petri dishes that can be seen in this photograph. This allowed him

to isolate and culture disease-causing agents (pathogens).

Pin points are created in other ways besides the use of Grander® Technology. Pin points can also form when a water sample is sub-jected to ultrasound over a longer period of time. Under the influence of very high frequency sound waves, bacteria “families” are fragmented and scattered. The individual colonies thus formed often take the form of extremely tiny colonies, thus becom-ing pin points.

A second possibility of producing pin points is the use of sub - lethal radiation with ultraviolet light. Also

in this case, the introduction of en-ergy can tear apart the aggregations of bacteria.

Grander® Technology uses a com-pletely different principle in induc-ing pin point formation. It does not depend on massive doses of energy. Rather, according to our present state of knowledge, by altering the cluster structure of the water the behaviour of the micro-organisms is fundamen-tally changed. Thus the Grander® pin points that form are a reaction to the structural change of the water and not the result of some process that leads

to the destruction of the bacterial aggregates.

For this reason the pin points that result from ultrasound influence are fundamentally different from Grand-er® pin points. If a water sample is subjected to ultrasound and then tested for bacteria, tiny colonies can be found almost immediately, just as soon as they have had time to multiply.

Following contact of a water sample with Grander® Technology it takes at least two to three days before pin points are formed.

What Causes Pin Points To Form?

drawn through a membrane filter under sterile conditions. The bacteria and fungi contained in the water are trapped by the filter, which is then laid onto an artificial culture me-dium. The filter draws nutrients from the culture medium so that the bacteria and fungi found on the surface of the filter easily grow and multiply. The bacteria multiply in a concen-tric pattern, ideally spreading from a single bacterium and forming colonies that are visible to the naked eye. The reason this happens is that some 100,000 bacteria can be generated from a single bacterium in a period of only 48 hours (depend-ing on the reproductive period). The formation of visible colonies makes it possible to take a bacteria count even with-out the assistance of a microscope. The great advantage of this method is that only those bacteria and fungi capable of reproducing are counted, i.e. the only germs that represent a possible danger in drinking water.

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The Pin Point Formation of Bacteria, Using Grander® Technology

The pin point formation that gives revitalised water com-pletely new qualities is one of the most fantastic phenom-ena related to Grander® Technology. With amazing speed these pin points break down the organic content dissolved in the water, thus purifying it. In this manner the water loses its perishability and regains all those qualities that it had in an unpolluted state.

The astonishing thing about this phenomenon is that water that has once been revitalised by the Grander® me-thod always retains these qualities. If a bottle of Grander® water is polluted, for example, by adding a drop of a sterile,

Membrane filter (diameter 50 mm, thickness

.01 mm) with a printed grid for counting. Be-

cause of their size, bacteria and fungi cannot

pass through the pores and are trapped by the

filter. After 24, 48 and 96 hours the colonies

that have formed are counted.

After the sample has been drawn through the

membrane filter, it is “rolled” onto the surface

of a sterile culture medium. During the incuba-

tion period the bacteria found on the filter are

nourished by the culture medium, enabling

them to reproduce and form colonies.

This procedure is described in DIN

38411-K5. The membrane filters consist

of chemically modified cellulose and

have a pore size of 0.45 µm. These pores

are thus too small for most bacteria to

pass through so that they are trapped

on the surface of the filter. In each test,

1 ml of sample liquid is drawn through

the membrane filter, which is then laid

onto a standard culture medium.

“Determination of Microbes Capable of

Reproduction, Using the Membrane Filter Method”

Distinguishing Features between Tiny Colonies, Created by the Effects of UV Radiation or Ultrasound, and Grander® Pin Points:

Pin point formation due to Pin point formation due to Grander®

ultrasound or UV radiation

Pin point formation induced by mechanical sound energy or heavy altered microbiological behaviour; no

radiation, causing splitting of splitting of bacteria aggregations into

aggregations into individual bacteria individual parts, but alteration into

harmless but very active new forms

Occurrence immediate not until 2 to 3 days after first

contact with Grander® Technology

Substrate utilisation same as “wild” bacteria different utilisation than

“wild” bacteria

Breakdown of carbon content no increased breakdown and/or increased breakdown and lowering

and lowering of AOC content no lowering of AOC content of AOC content, resulting in

(food content) purification and improved water

artificial culture medium, the number of pin points incre-ases within only a few hours. They quickly break down the source of carbon, thus purifying the water once again.

With regard to pin point formation Grander® Technolo-gy is highly specific. I have tested many of the water systems available on the market. They promise water activation or similar effects, but not a single one of these technologies was able to promote pin point formation in Johann Grander’s sense.

Because I continue to refer to the high number of pin

points, I should point out once again the harmlessness of these pin points. The changes they undergo makes pin points lose the qualities of resistance that the “wild” bacteria once had. It is hardly possible for pin points to

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After an incubation period of 96 hours, colonies

are formed that can be seen with the naked eye

and thus can be counted. This drinking water

had 30 CFU/ml (CFU = colony forming units).

The guideline that may not be exceeded under

Austrian regulations sets a limit of 100 CFU/ml.

Water revitalised using Grander® Technology

shows typical pin point formation (these are

the very small white colonies). The unrevitalised

colonies = “wild” or mother colonies are sub-

stantially larger and have been coloured red by

a dye in the culture medium.

Example of unrevitalised water with acceptable

bacteria levels. In order to create more colonies,

50 ml was drawn through the filter, which was

then incubated on standard nutrient agar for

72 hours at 22 degrees Celsius. The various

sizes and shapes of the colonies are typical.

Following revitalisation of water from St Ulrich

am Pillersee and two resting days, this typical

image was formed after 72 hours of incubation

at 22 degrees Celsius. Only a few “wild” colonies

remain. A large number of Grander® pin points

predominate.

When the water has been completely revital-

ised by Grander® Technology, only pin points

are found on the membrane filter. No “wild”

colonies are to be seen.

Proof of Effectiveness of Revitalisation

multiply at 37 degrees Celsius no matter how good the culture medium is. Thus we may be certain that drinking water can never have a harmful effect, no matter how ma-ny pin points it may contain, because both normal body temperature and stomach acid would kill these pin points immediately. In addition, an increase in the number of pin points does not imply an increase in disease germs (which are not permitted by law).

Details on Grander® Pin point Formation

As early as 1993 I was able to establish that bacteria contained in water form pin points as a result of Grander® Technology. If a sample of drinking water, for example,

comes into contact with Grander® Technology, one can observe the pin point formation taking place within about two to three days (that’s how long the bacteria take to change). The bacterial picture becomes uni-form: there are no longer any large “wild” colonies, but only small ones of pin-head size.

This pin point formation, which is typical of Grand-er® Technology, has meanwhile also become the most important method for demonstrating the effectiveness of Grander® Technology.

Since 1993 I have conducted tests on some 2,000 wa-ter samples and found confirmation time and time again of pin point formation caused by Grander® Technol-ogy. This pin point formation has also been confirmed

The completely different im-

ages shown here make the re-

markable nature of Grander®

Technology with respect to pin

point formation perfectly clear.

This discovery has made it pos-

sible to develop a comprehen-

sible scientific procedure for

determining the effectiveness

of Grander® Technology in

water systems.

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by other university institutes and by Prof. Dr. Rachmanin, the man responsible for the purity of Moscow’s city water supply.

What Qualities Are To Be Found in the Pin Points Resulting from

the Use of Grander® Technology?

It is truly fascinating how the number of pin points increases sharply within just a few days of revitalisation with Grander® Technology. One might easily assume that the pin points I describe are the result of some externally caused infection or contamination. But this assumption can be refuted in a very simple experiment:

A sealed bottle, made of glass or plastic and containing the water to be tested, is placed for two to three days next to a bottle of Original Grander® Water. Bacteriological examination after an appropriate period of incubation reveals the pin point formation described here. A blind test with a second bottle filled under the same conditions but with no contact with Grander® Technology demonstrates the difference: there is no formation of pin points. The only case in which pin points are not formed is if the water has been strongly chlorinated and thus was bacte-riologically dead when the sample was taken. In the mem-brane filter method, if the pin points form very slowly on the agar plate, i.e. only after 96 hours of incubation, this is always an indication of the presence of inhibiting chemicals in the water, such as disinfectants or unusually high concentrations of heavy metals.

Thus the formation of pin points using Grander® Tech-nology cannot be explained as a bacterial infection. Rather there is alteration of the micro - organisms, giving them completely new qualities with respect to their heightened ability to break down the bacterial nutrients contained in the water.

Alteration of the Qualities of Resistance of Pin Points

Evidently, the pin points resulting from Grander® Tech-nology also lose the qualities of resistance that characterised the original “wild” colony. I was able to establish this my-self in the case of an 80,000 - litre closed heating system in a hospital. The system operated at a primary temperature of 55 degrees Celsius and a return - flow temperature of 48 degrees Celsius. These are temperatures at which aquatic pseudomonads cannot survive. Nevertheless, a very high level of microbe contamination was found in the circulat-ing water. Apparently, high selection pressures had result-

5 ml of unrevitalised drinking water

was drawn through the membrane

filter and incubated for 96 hours at

22 degrees Celsius. One can see the

diverse nature of colony formation, the

varying colour (because of a colorant

added to the culture medium) and the

varying size of the colonies. This water

afterwards was revitalised with the

help of Grander® Technology.

Five days after the start of revitalisa-

tion, the bacteriological picture has

already changed. The size of the col-

ony formations no longer varies, and

the colours have become uniform.

Ten days after the start of revitalisa-

tion, countless pin points have formed.

The colonies are so close together

that only the outer edge of the thick

growth is capable of accepting dye.

There is very thick growth at the inte-

rior of the filter area.

Fifteen days after the start of revitalisa-

tion the number of pin points becomes

smaller. This is a sign that the food sup-

ply has almost been exhausted and the

bacteriological purification has for the

most part been completed.

Four weeks after the start of revitalisa-

tion using Grander® Technology the

food supplies have been completely

exhausted. The pin points have died.

The water is biologically pure. Because

of the sharply lower levels of assimila-

ble organic carbon (AOC), bacteria can

no longer reproduce (below 10 µg org.

Celsius/ml).

Alteration of the Colony Image by Grander® Revitalisation

First, unrevitalised drinking water was incubated for 96

hours at 22 degrees Celsius. After revitalisation, the colony

image clearly changed, as may be seen in the photographs

taken after 5, 10, 15 days and 4 weeks.

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ed in the development of temperature-resistant plasmids (small, circular DNA-molecules), permitting survival at temperatures of around 50 degrees Celsius. The system was revitalised using Grander® Technology. Six weeks later no bacteria were to be found in this closed heating system. (A second bacteriological institute, which was asked to become involved in the experiment as a result of insurance issues, arrived at the same results.) Evidently the temperature resistance of the “wild” bacteria was lost when they were transformed into pin points. Fundamentally, pin points formed as a result of Grander® Technology show a striking sensitivity to temperature. When water that has been revi-talised using this technology is examined and the culture medium has been incubated at 37 to 40 degrees Celsius, there is no colony formation. However, at room temperature pin points develop on the culture medium in the usual form after 24 hours.

The Utilisation of Substrates by Grander® Pin Points

Of particular interest is the ability of pin points created through the use of Grander® Technology to find new sources of nourishment. In one example, Grander® Technology has been used for many years to improve closed cooling systems that are operated either with pure water or with hydrocarbon coolants – mineral-oil products.

One user of such hydrocarbon coolants had the idea of revitalising a supply barrel of readymade hydrocarbon coolant in order to see what the effect would be. Grander® revitalisation equipment was suspended in a sealable 200-li-tre steel drum containing 180 litres of coolant emulsion, and the drum was closed. It was only opened, when necessary, to take samples for bacteriological examination. The revitali-sation process followed the expected bacteriological course: within only a short time after the Grander® Technology revitalisation began, there were a large number of pin points. A peak was reached after four weeks. After six weeks the number began to fall, reaching a minimum after eight weeks. I came to the conclusion that the easily utilised sources of carbon had been exhausted.

After 10 weeks, however, the number of pin points began rising again. From this point on, my laboratory tests provided the interesting information that the mineral-oil content of the emulsion, which had earlier been .8 per cent, was steadily decreasing. Evidently, the pin points, as their primary source of nourishment was becoming exhausted, had learned to crack the more complex carbon chain structures and use them as sub-strates. The explanation for this is that, in contrast to the usual circulatory cooling systems in which nutrients are constantly being added, in this drum there was a

limited food supply, and as it became exhausted, the pin points began using the oil as a food source.

For many years now in my laboratory I have been checking closed cooling systems equipped with Grander® Technology. In practice, pin point formation does not lead to an undesirable breakdown of the oily components. This is because the pump circulation of the cooling sys-tem provides sufficient sources of food that can easily be broken down.

The Nutrient Content of Water Is Lowered by

Grander® Pin Points

If bacteria is to remain alive in drinking water, the water must also contain nutrients. In the 1990s, Dutch scientists set out to determine the necessary nutrient supply in water (primarily in drinking water). This nutrient package is called assimilable organic carbon (AOC) and is expressed in µg carbon equivalent per litre.

The lower limit required for bacterial growth has been determined to be an AOC of 10 µg per litre. What does this mean in practice? If drinking water contains less than 10 µg AOC per litre, there are not enough nutrients. Over the course of time the bacteria living in the water will simply starve. In practice it has been determined that drinking water with an AOC content of 10 to around 50 µg/l may be classified as good, having only a small tendency to support bacterial growth.

At the Department of Hydraulic Engineering, Indus-trial Water Management and Prevention of Water Pollu-tion, Agricultural University, Vienna, a bacteriological examination of Original Grander® Water was recently conducted under the supervision of Dr. Franziska Zibus-chka. A water sample was bottled on January 17, 2000. This sample was tested on February 2, 2000. The exami-nation revealed an unheard-of AOC figure of only 1.8 µg carbon equivalent per litre, lower than ever before. Un-der these conditions, bacterial growth is impossible and, as long as the bottle is not opened, the water will keep virtually forever. The explanation for this extremely low AOC figure is pin point formation induced by revitalisa-tion. The pin points have consumed almost all the nutri-ents in the water, so that the bottled Original Grander® Water no longer tends towards bacterial growth. The stability tests are now continuing in their sixth year. Original Grander® water bottled in March 1994, although it has been kept at room temperature, remains bacterio-logically pure, meeting all the parameters of chapter B 17 of the codex, “Table Water”.

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products. Above all, bottling water using Grander® revi-talisation equipment is far more effective in its function of transmitting information across wide distances and large surfaces. This high revitalisation effect is also necessary, considering that tap-water often passes through the equip-ment in only a fraction of a second.

For test purposes, we have removed Grander® revi-talisation equipment from water lines, and with the help of the pin point method we tested how long it would take before the level of revitalisation dropped. Revitalisation slows down only after a period of one to two months. Evidently information is stored throughout the entire drinking-water system – a function of the materials used in the pipework.

This revitalisation effect of Original Grander® Technol-ogy, which also includes the surrounding area, sometimes creates difficulties in a research laboratory – especially if unrevitalised water is needed for purposes of comparison and much of the glass equipment being used has already been revitalised through contact.

In principle, a good source of drinking water is also characterised by a low water temperature, which ideally lies between five and eight degrees Celsius. Because of this low temperature the few bacteria found in this water almost appear to be dead. Of note is the sharp increase in their reproductive period, the time a mother cell takes to divide into two daughter cells. Under ideal circumstances – growing on the best culture medium and at room tem-perature – this reproductive period takes about 20 min-utes. In the case of drinking water at a temperature of six degrees Celsius the reproductive period increases to around 10 to 12 hours. The bacteria become more active only after the water has been warmed to room tempera-ture.

The uniqueness of Original Grander® Water lies in the fact that it is possible to store this bottled water without the addition of preservatives (i.e. carbonic acid) for many years at room temperature, and it remains fit for consump-tion.

Contact Revitalisation Using Grander® Technology

Earlier we talked about a bottle containing Original Grander® Water being placed next to a sealed bottle of water that had not been revitalised and the fact that this contact brought about revitalisation after only a few days. Pin point formation was found in the water that had origi-nally not been revitalised. Our experience is that contact with Original Grander® Water brings about revitalisation in a lessened, diluted form.

This contact-revitalisation represents the elemental and fundamental basic principle of the mode of action of Grander® revitalisation equipment and all other Grander®

It is still impossible to foresee the ultimate significance of Grander® pin point formation in its final conse-quences. In any case, this technology makes it possible to bottle drinking wa-ter without the addition of chemicals and to store it for years without loss of quality. Enormous drinking-water reservoirs, set aside for times of emer-gency, could be preserved in a bacter-iologically stable state without the use of chemical additives. The same ap-plies to drinking-water supplies that

are maintained for use aboard ships, planes, caravans or dining-cars.

The implementation of Grand-er® Technology at public swim-ming-pools has demonstrated that pin points show substantially more sensitivity to chlorine than do “wild” bacteria. Thus it is possible to safely reduce the chlorine content of pool water to the minimum limit of .3 mg chlorine per litre required by swim-ming-pool regulations.

The implementation of this tech-

nology in the field of rainwater-recov-ery systems has led to a reduction of offensive odours and to the settling of particles suspended in the water. Above all, Grander® pin points are highly capable of reducing unpleasant odours, the cause of which is usu-ally the activity of anaerobic bacteria. Evidently, oxygen conditions are im-proved by structural changes in the water following revitalisation with Grander® Technology, thus eliminat-ing anaerobic conditions.

The Significance of Grander® Pin Point Formation in Practical Application

Summary

The bacteriological tests that have been performed were in-

tended to demonstrate that Grander® Technology brings

about a bacteriological alteration in the water. Evidently,

the “wild” bacteria react to the changed conditions brought

about by Grander® Technology in the form of the pin

point formation described here and reduce the nutrient

content of the water. This leads to a comprehensive pal-

ette of new and interesting qualities in water that has been

revitalised with the help of Grander® Technology.

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I n searching for biological test models to explain the pos-itive alteration of pollutant information by Grander®

Technology, I concentrated primarily on investigative methods that are generally accessible or whose procedures have been standardised. It was important to find a method that could be duplicated in any laboratory furnished with the proper equipment. This test report is an excerpt from a more comprehensive investigation. Details of my testing methods may be obtained from me at any time.

The American researcher A. A. Bulich established that phosphorescent bacteria are extremely sensitive. Even in the presence of minute quantities of toxic material the phospho-rescent intensity is reduced. In 1979 it occurred to him that these bacteria might be used to determine the toxicity of pol-luted water. He succeeded in measuring the bioluminescence of the bacteria.

Summarised in a single paragraph, the principle of mea-surement seems very “enlightening”: the phosphorescent bacterium radiates with full intensity when it is doing well. But in the presence of even the smallest quantities of toxic material

it phosphoresces with diminished intensity. The difference in phosphorescent output can be measured.

The Phosphorescent Bacteria Test – a Standardised Procedure

In the meantime, the phosphorescent bacteria test has proved itself to such an extent that in 1991 the procedure was standardised. According to DIN 38412, Part 34, it is possible to test waste water using phosphorescent bacteria. Even though this norm was developed for pollutants in waste water, it is applicable to every other kind of water as well, including drinking water, and even drainage water from dumps.

Material and Method

The phosphorescent bacteria used for testing were obtained from the firm Dr. Lange (where they are called LUMISmini). They are stored at a temperature of -18 degrees Celsius. With

Biological Method for Proving the Positive Alteration of Heavy Metal Information in Water, Using Grander® Revitalisation

The Phosphorescent Bacteria Test

Johann Grander has said: “Grander® Technology works with natural energy. Because of this quality it is able to reverse the polarity of negative information so that it can no longer

have a negative effect. It is possible to neutralise negative information only by using energy and information that have come from nature itself.” The present work is an attempt to

provide microbiological proof of Johann Grander’s statement.

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the reactivation liquid, which is supplied at the same time, bacteria can be prepared for testing in only 15 minutes. Along with these reactivated phosphorescent bacteria, tests are also performed on bacterial suspensions that are prepared in the laboratory.

The luminescence measurements are conducted using a measuring device from the firm Dr. Lange called LUMISmini Type LPG 298. Two kinds of measurement can be recorded with this device: the actual phosphorescent output, or the variation (increase or reduction) in phosphorescent output relative to a pre-selected standard value. A single measure-ment takes about 10 seconds. In every experiment a standard sample consisting of unrevitalised natural drinking water containing 2 per cent sodium chloride is also tested.

Lab station for phosphorescent bacteria tests.

In the foreground are two LUMISmini photometers, behind them a

thermoblock for maintaining the suspension at a fixed temperature.

Measurements are taken in disposable glass bowls at

15 degrees Celsius and at 21 degrees Celsius.

Detail of a LUMISmeter with which the phosphorescent output

(bioluminescence) of a phosphorescent bacteria suspension can be

measured. The instrument records the reduction in phosphorescent

output as a percentage compared with a standard value.

Bioluminescence

Everyone is familiar with the female firefly, who sets out

at twilight on mild May evenings to attract male fire-

flies with her “lantern”. In some species, female fireflies

(glow-worms) are incapable of flight. For that reason they

climb onto blades of grass, sending out their characteristic

greenish-yellow light. They do so in hope of attracting an

airworthy male partner, who responds with a light signal

of his own.

The aquatic phosphorescent bacteria of the types called

Photobacterium phosphoreum and Vibrio fischeri are found in

the sea, where they broadcast a bluish fluorescent light

with a wavelength of 490 nanometres. This biolumines-

cence (light emission by an organism) is directly depend-

ent on the metabolic (variable) condition of the bacterial

cells. The emissions are particularly strong when they

are doing well.

The Special Qualities of Phosphorescent Bacteria

• Bacteria in general, and marine bacteria in particular, are the oldest known living creatures, and yet they are evi-dently particularly sensitive to pollutant substances. • Phosphorescent bacteria usually react sensitively and quickly. This means that a measurement taken after only 30 minutes often results in a definite conclusion. • Around one million bacteria are used in a single phos-phorescent bacteria test. This means the test examines the behaviour of one million individual cells.• The phosphorescent bacteria test can be standardised using the methods of quality control. This means that definite and comparable – and above all repeatable – re-sults can be produced.

This photograph of phosphorescent

bacteria under a microscope shows

that there is luminescence outside

the cell nucleus. The strength of

the bioluminescence is a measure

of the positive metabolic condition

of the bacteria. Toxic substances re-

duce the phosphorescent output.

In the dark, the phosphorescence

(bioluminescence) of the bacteria

can be observed particularly clearly

with the naked eye. With the addi-

tion of the appropriate amount of

salt, phosphorescent bacteria can

be cultured on laboratory plates.

That permits the production of

fresh bacteria suspensions that

show a particularly high sensitivity

to toxic substances.

12 13

Lead in Drinking Water30-minute duration of action – freshly cultured phosphorescent bacteria

The Test Method

In this series of tests, unpolluted, non-revitalised drink-ing water is contaminated with heavy metals. Since these phosphorescent bacteria are marine creatures, it is necessary to add 2 per cent sodium to the water being tested. This water sample is then divided into two equal parts. One part is revitalised by dipping a Grander® Energy Rod into it for about 10 seconds; the second part remains unrevitalised. Thus the composition of both water samples is completely identical. Revitalisation with a Grander® Energy Rod (filled with Grander® Information Water) neither adds anything to the water nor removes anything from it. Both water samples are then subjected to the phosphorescent bacteria test. The bacterial suspension used for the test is distributed in the same quantity and concentration in both test series, and the phosphorescent output is measured at the same temperature and after the same period of time. This is the basic concept for the test series.

Phosphorescent Bacteria Test of Water with Lead Concentrations

between .0625 and 1 mg/lNon-revitalised drinking water with a lead content below

the analytically detectable level of 0.01 mg/l l was mixed in the laboratory with lead salt (lead nitrate), so that the following concentrations were achieved: 0.0625 mg/l, 0.125 mg/l, 0.25 mg/l, 0.5 mg/l and 1.0 mg/l.

The highest concentration permitted in drinking water

lies at present at around 0.05 mg/l. Thus the water samples tested here would have been, with the exception of the first sample, considered unpotable.

Results

The beams below the 0%-line on the ordinate represent inhibition of the phosphorescent output, the beams above an increase in phosphorescent output – a result of Grander® revitalisation. These increases in phosphorescent output occur in concentrations below 0.25 mg lead per litre of drinking wa-ter. Evidently, this is a catalytic effect on the biological process responsible for phosphorescent output.

The chart indicates the effect on non-revitalised water after a period of 30 minutes. A concentration of 0.0625 mg lead per litre of drinking water experiences no significant reduction in phosphorescent output. At a concentration of 0.125 mg lead per litre the reduction is 6%, while at a concen-tration of 0.25 mg lead per litre there is a 10% reduction. At concentrations of 0.5 mg lead per litre the reduction is 33%, and at a concentration of 1.0 mg lead per litre it is 52%.

After a wait of 60 minutes, the comparative values are -3%, -5%, -19%, -43% and -62%.

The chart also indicates the effect on Grander® revital-ised water after a period of 30 minutes. At a concentration of 0.0625 mg per litre of drinking water, there is an actual increase in phosphorescent output of 32%. At a concen-tration of 0.125 mg/l the increase is 14%, and at 0.25 mg/l

40%

30%

20%

10%

0%

10%

20%

30%

40%

50%

60%

red

uct

ion

or

incr

ease

in %

0.0625 mg/l 0.125 mg/l 0.25 mg/l 0.5 mg/l 1.0 mg/l

Grander® revitalised unrevitalised

32%

0%

14%

6%1%

33%

52%

10%

7%

17%

Concentrations

14 15

around 7%. A small reduction occurs at a lead concentra-tion of 0.5 mg/l with -1%; at a concentration of 1.0 mg/l the reduction is -17%.

After a wait of 60 minutes, the comparative values are +10%, +1%, -5%, -15% and -44%

Significant Differences to Non-revitalised Water

These test results show significant differences between water revitalised with Grander® and non-revitalised water when both contain similar concentrations of lead.

The values reached after 30 minutes are particularly inter-esting, while those reached after 60 minutes do not represent as significant a difference. This, however, does not mean that Grander® Technology is less effective, but rather that the effect of the poison is time dependent.

In lead-containing water that has been revitalised using Grander® technology, after 30 minutes concentrations of up to 0.5 mg lead per litre of drinking water show practi-cally no reduction of phosphorescent output. Not until a value of 1.0 mg per litre is an effect of -17% recorded. In non-revitalised water a reduction is already recorded at a concentration of 0.125 mg per litre and reaches -52% at 1.0 mg per litre.

Phosphorescent Bacteria Tests of Water with Mercury Content between 0.0007 and 0.07 mg/l

Non-revitalised drinking water with a mercury content below the analytically detectable level of .0005 mg/l was mixed with a mercury salt (mercury II sulphate) so that the following concentrations were achieved: 0.0007 mg/l, 0.0035 mg/l, 0.007 mg/l, 0.035 mg/l and 0.07 mg/l. The highest con-centration of mercury currently permitted in drinking water

Results in Samples Containing Mercury

0.0007 mg Hg/l 0.0035 mg Hg/l 0.007 Hg/l 0.035 mg Hg/l 0.07 mg Hg/l

unrevitalised 0% –10% –18% –51% –89%

Grander® revitalised -42% +28% +17% +13% –35%

50-minute duration of action – freshly cultured phosphorescent bacteria (1 ml sample + 0.4 ml bacterial suspension).

Lead in Drinking Water60-minute duration of action – freshly cultured phosphorescent bacteria

20%

10%

0%

10%

20%

30%

40%

50%

60%

70%

red

uct

ion

or

incr

ease

in %

0.0625 mg/l 0.125 mg/l 0.25 mg/l 0.5 mg/l 1.0 mg/l

Grander® revitalised unrevitalised

10%

3%

1%

5% 5%

19%15%

43% 44%

62%

Concentrations

14 15

0.0007 mg/l 0.0035 mg/l 0.007 mg/l 0.035 mg/l 0.07 mg/l

Grander® revitalised unrevitalised

is 0.001 mg/l. Thus the water samples that were tested were, with the exception of the first sample, were nonpotable.

Results

An increase in the phosphorescent output of Grander®-revitalised water samples was found at low concentrations of lead and cadmium as well The chart shows the results after 50 minutes. Compared with lead, mercury is substantially more toxic to phosphorescent bacteria, or to reverse the statement: phosphorescent bacteria are substantially more sensitive to mercury in solution than they are to lead.

In the sample that had undergone Grander® revitalisa-tion, an increase in phosphorescent output was seen in con-centrations of up to .035 mg/l. Not until concentrations of 0.07 mg/l and higher was there a reduction in phosphorescent output of around -35% in the revitalised sample as well.

In the unrevitalised water containing mercury there

Results in Samples Containing Cadmium

0.005 mg Cd/l 0.01 mg Cd/l 0.05 Cd/l 0.1 mg Cd/l 0.25 mg Cd/l

unrevitalised 11% –20% –31% –38% –53%

Grander® revitalised +21% +11% –7% –20% –47%

60-minute duration of action – freshly cultured phosphorescent bacteria (1 ml sample + 0.4 ml bacterial suspension).

Mercury in Drinking Water50-minute duration of action – freshly cultured phosphorescent bacteria

60%

40%

20%

0%

20%

40%

60%

80%

100%

red

uct

ion

or

incr

ease

in %

Concentrations

42%

0%

28%

10%

17%

18%

13%

51%

35%

89%

was a small reduction already at 0.0035 mg/l. The biggest difference between the Grander® revitalised sample and the unrevitalised sample was at a concentration of 0.035 mg/l. An increase in phosphorescent output of around +13% was seen in the revitalised sample; in the unrevitalised sample the reduction was -51%.

Phosphorescent Bacteria Test of Water with Cadmium Concentrations between

0.005 and 0.25 mg/l

Analogously to the previous tests, cadmium-free drinking water was mixed with cadmium ions in the form of cadmium chloride monohydrate, so that the concentrations 0.005 mg/l, 0.01 mg/l, 0.05 mg/l, 0.1 mg/l and 0.25 mg/l were reached. The present maximum permissible concentration of cadmium in drinking water is 0.005 mg/l.

16 17

Reduction in Phosphorescent Output according to Koller-Kreiml and Rodinger, 1987

% of reduction after 30 minutes duration of action Assessment

under 10% insignificant reduction

10% - 40% moderately strong reduction

40% - 60% strong reduction

60% - 90% very strong reduction

over 90% complete elimination

Cadmium in Drinking Water60-minute duration of action – freshly cultured phosphorescent bacteria

30%

20%

10%

0%

10%

20%

30%

40%

50%

60%

red

uct

ion

or

incr

ease

in %

0.005 mg/l 0.01 mg/l 0.05 mg/l 0.1 mg/l 0.25 mg/l

Grander® revitalised unrevitalised

21%

11%

11%

20%

7%

31%

20%

38%

53%47%

Results

The phosphorescent bacteria display a sensitivity to cadmium ions that is greater than in the case of lead ions but not as high as in the case of mercury ions. The greatest difference between Grander®-revitalised and unrevitalised water containing cadmium is evident at concentrations of 0.005 mg/l, 0.01 mg/l and 0.05 mg/l. No significant differ-ence between the Grander®-revitalised and unrevitalised samples was measured at 0.25 mg/l. This concentration is too high for Grander® revitalisation to result in a reduc-tion in toxicity to phosphorescent bacteria.

The Statistics of the Analysis Method

Ring tests reveal that the coefficient of variation – depending on what is being measured – varies between

6% and 16%. In measuring the same heavy metals and in 10 individual measurements under completely identical condi-tions the deviation was on the order of ±5%.

In the phosphorescent bacteria test it has become estab-lished practice that reductions amounting to less than 20% are not the subject of further discussion. In such cases the test medium is considered to be of low toxicity. This is also taken into account in the test results presented here. Specifically: differences of less than 20% are not considered to be particu-larly meaningful.

What Is the Meaning of the Test Results and How Should

They Be Used?

The three “most hazardous” heavy metals in drinking water (lead, cadmium and mercury) were examined in the

Concentrations

16 17

phosphorescent bacteria test and significant reduction differ-ences between unrevitalised and Grander®-revitalised water were established. After elimination of all sources of error, these differences were attributed to a positive information change caused by Grander® Technology.

By no means should these first results be used to promote Grander® Technology as a method for lowering “heavy metal pollution”. Since Grander® revitalisation equipment is used in the field of drinking water only when the water is potable, there is no fundamental reason to even consider it for such a use.

The phosphorescent bacteria test is an interesting biolog-ical test model for establishing information changes. Many additional tests will be necessary in future. For when a posi-tive information change has been established, this change will apply not only to the heavy metal content of the drinking water but will be generally applicable to all other contents within a certain level of concentration.

For example, when nitrate concentrations of up to 500 mg/l were tested, the phosphorescent bacteria always reacted with an increase in phosphorescent output. Nitrates are evi-dently food sources for phosphorescent bacteria. Nitrates are also ubiquitous in seawater. Thus this result should come as no surprise.

Summary

With the help of phosphorescent bacteria tests standard-

ised according to DIN, this highly sensitive biological mod-

el was used for the first time to examine the question:

Can a positive alteration of pollutant information as a

direct result of using Grander® Technology be scientifi-

cally established?

In the case of drinking water samples that were artificially

polluted with lead, mercury and cadmium, the inhibition

was significantly smaller in the Grander®-revitalised water

at lower concentrations than in unrevitalised water.

Despite these results, however, Grander® Technology

should not be used in an attempt to remove toxins from

water containing heavy metals.

The experiment confirmed that phosphorescent bacteria

are well suited for use in such examinations. The research

work will be extended to a large number of other pollut-

ants found in drinking water in order to further confirm

the correctness of the findings.

Reference to Further Reading• Bulich, A. A. Use of Luminescent Bacteria for Determining Toxicity in Aquatic Environments, in: L. L. Marking, R. A. Kimerle: Aquatic Toxicology, ASTM STP, American Society for

Testing and Materials, vol. 667, pages 98-106, (1979)

• Dannenberg, R.: Water - Waste Water, 135 no. 8, 475-480 (1994)

• Kaiser, K. L., Palabrica, V. S., Photobacterium Phosphoreum Toxicity Data Index. Water Pollution Research Journal of Canada, 26, 361-431 (1991)

• Koller-Kreiml, V. and Rodinger, W., Aquatische Toxizität - ein wichtiges Kriterium zur Beurteilung von Substanzen in Abwässern (Emissionen) sowie zur Feststellung der toxischen

Beeinträchtigung von Oberflächengewässern (Immissionen). Wasser und Abwasser 31 413-432 (1987)

• Coleman R. M., Qureshi, A. A. Microtox: Reserved and Spirillum Volutans Tests for Accessing Toxicity and Environmental Samples. B. Env. Contam. and Tox. 35, 443-451 (1985)

• Nohava, M. “Der Leuchtbakterientest in der Umweltkontrolle.” Untersuchungsbericht des Umweltbundesamtes UBA-1994-0909, February 1994, page 21.

18 19

Chapter B17 of the Austrian codex of food regulations pertains anytime drinking water is bottled and of-fered for sale in Austria. It establishes that a bottle

of water has to meet the following guideline figures 12 hours after it has been freshly filled:

• max. 100 CFU/ml at 22° Celsius / 72 hours incubation time and

• max. 20 CFU/ml at 37° Celsius / 24 hours incubation time.

The most important requirement, however, is that in a 250 ml water sample there must be no microbes harmful to health (these are prohibited by law).This specifically relates to Escherichia coli, coliform bacteria, enterococcus bacteria, Pseudomonas aeruginosa and Staphylococcus aureus. In addition, the level of sulfite-reduced clostridia in 50 ml of water must be undetectable.

These regulations establish requirements for the qual-ity of bottled table water; there are separate requirements for mineral water. Austrian law was adapted to European Union law in the Federal Law Gazette 309 of 1999, which was published on January 13, 2000. A consumer, of course, almost never has a bottle that was filled only 12 hours earlier. Usually the water has been on the shelf for some time before it ar-rives on the consumer’s table. What guidelines apply to these

water samples, which are usually somewhat older? Chapter B17 of the codex has nothing to say about this. Publications that address this field permit guideline figures of up to 1000 CFU/ml if the microbes that are harmful to health, as men-tioned earlier, are absent. Here something clearly needs to be done to protect consumers.

The Problem of Later Germination

As a reminder: for micro-organisms to survive in drink-ing water, the water must contain nutrients. This nutrient package is called AOC (assimilable organic carbon) and is expressed in µg carbon equivalent per litre. The lower limit needed for bacterial growth has been determined to be an AOC of 10 µg per litre; this is the AOC value below which bacteria cannot reproduce.

In producing and bottling table water, the germination problem would be solved if only water samples with an AOC content of less than 10 µg per litre were used. However, such water samples are rather rare. Even very good drinking water usually has a higher AOC content.

What is done in practice to solve this problem? Carbonic acid is added, which lowers the pH to less than 5, thus inhib-

Grander® Technology Improves the

The Preservation Qualities of Drinking Water

My article “Water for Eternity”, which was published in the Grander Journal, vol. 1, 1999, (pages 42-47), provides additional information on this topic. Here I present the most important

findings of that report, along with the most recent test results.

Phot

os:

Dr.

Fel

sch

18 19

iting the reproduction of aquatic pseudomonads. Consumers often find that the addition of this preservative gives water a particularly tingling and refreshing taste. The actual taste of the water, however, is hardly discernible.

Water Is Less Perishable with Grander® Technology

Countless tests have shown that Grander® Technology, without the use of additives or other measures, is capable of stabilising and permanently preserving drinking water even at room temperature. Grander® revitalisation leads to a breakdown of organic nutrient sources in the water. One of the effects of Grander® Technology is that the AOC content in the water is quickly broken down, thus preventing the growth of microbes in the water.

How Can the Nutrient Breakdown Be Explained?

It would appear that the nutrients stored in non-revital-ised drinking water are less attractive to bacteria. Even if the temperature of the source water is raised from eight degrees Celsius to 25 degrees Celsius, the bacteria do not become substantially more active. A rise in the microbe count from 5 CFU/ml to 140 CFU/ml within a month had almost no effect on the AOC content.

The Grander® pin points, on the other hand, are substan-tially more active, and lower the AOC content by 90%. Follow-ing proximity revitalisation using Grander® Technology, micro-organisms contained in the water begin to alter their behaviour. Suddenly the food supply in the water appears to become very usable by the pin points that have developed in the interim. The pin points react by multiplying sharply and consuming all the available food. Thereafter, the pin points en-ter an inactive phase and assume the function of a “surveillance

Breakdown of Organic Food Sources

The following experiment serves to explain the phenom-enon of the rapid breakdown of AOC:

Three samples were taken from a drinking-water source known to be of high quality:• The first sample (unrevitalised) was immediately sent in

a cooled state to a health institute in order to determine its bacteriological quality.

• The second sample (unrevitalised) was sent to another university institute, which was asked to determine its AOC content.

• The third sample was divided and placed in two steril-ised glass bottles.

• One glass bottle was stored in such a way that it did not come into contact with Grander® Technol-ogy.

• The second bottle was placed next to a bottle of Orig-inal Grander® Water and contact-revitalised.

These two samples underwent bacteriological testing at one-week intervals and the number of colony forming units (CFU) was determined.

After one month all the test results were available: • First sample: the health institute found a total microbe

level of 5 CFU/ml after 72 hours at 22 degrees Celsius. At 37 degrees Celsius the level of CFU/ml was undetectable. The tests to determine the level of harmful microbes found none at all.

• Second sample: the AOC content was found to be 125 µg organic carbon per litre.

• The unrevitalised sample stored at room tempera-ture for one month showed a microbe increase from 5 CFU/ml (starting-point) to 140 CFU/ml.

• In the first week, massive pin point formation was found in the contact-revitalised Grander® sample. In the second week, 1,500 pin points per ml were counted. Starting in the third week, the number the pin points began to fall. After four weeks fewer than 10 pin points were detected.

AOC determinations were conducted again on the revitalised and the unrevitalised samples (both four weeks old) producing the following results: • Drinking water sample, unrevitalised, stored one

month at room temperature: AOC content of 120 µg/l – representing a drop of 4%.

• Drinking water, Grander®-revitalised, stored one month at room temperature: AOC content of 12 µg/l – repre-senting a drop of 90%.

Filled on March 13, 1994, this

bottle is now six years old.

Meanwhile, the date of manu-

facture is being printed on the

bottles, with the expiry date

given as two years later, but this

could be extended to six years.

The advice “store in a cool

place” is no longer necessary.

20 21

troop”. This troop immediately reactivates when nutrients appear. This is proved by the following experiment. A single drop of sterile bouillon solution is added to Grander® -revital-ised drinking water with an AOC content of 12 µg/l. This so-lution is stored for two days at room temperature. A microbe count will then reveal the following results: about 12,000 pin points have formed on the membrane filter after an incubation period of 72 hours at 22 degrees Celsius. Grander® revitalisa-tion and the high activity of these pin points thus guarantee complete natural purity of the water.

Mode of Action of Grander® Technology in the Bacteriological Stabilisation of Drinking Water

The previous experiment was based on a closed system: for example, drinking water that has been bottled so that there can be no further addition of nutrients or pollutants. Revitalisation of water samples in closed systems will result in the long-term stability, as described.

But what is the situation in the case of open systems, such as drinking-water pipes? Here fresh quantities of water are flowing through the revitalisation equipment, constantly bringing new nutrient materials with them.

Experience has shown that over the course of about two months after Grander® Technology has been installed, an equilibrium is reached. The number of pin points settles down to a value that is dependent on the AOC content of the drinking water. As was already mentioned in the previous chapter on pin point formation, within about two months of the installation of revitalisation equipment in a drinking-water pipe, the entire system of pipes will also have been revitalised. This is most likely a contributing factor to the

good revitalisation results achieved in practice. In stagnant water the activity is often even more intense. More pin points are usually found there than in samples of flowing drinking water.

Original Grander® Water - Found to Keep for Six Years

The sample bottle of Original Grander® Water concerned was bottled on March 13, 1994, and has been storedat room temperature for use in long-term stability tests.

Only four weeks after the bottle was filled – mid-April 1994 – the total microbe count of the water had fallen to an undetect-able level, and the microbe count has not changed to this day. Microbes harmful to health have never been present. Thus even after six years, this Original Grander® Water meets the standards of chapter B 17 of the Austrian codex of health regulations. In addition, samples have been taken since 1994 of every batch in order to test its state of preservation. This individual bottle is not unique in its capacity to keep for many years; this property has been confirmed by many other sample analyses.

The Course of Colony Formation in Revitalised and Unrevitalised Water

700

600

500

400

300

200

100

0

Days 0 3 5 13 20 30

650

390

100

0

25

160

200

180

190 210

14 10 5 0

mic

robe

cou

nt

mother colonies unrevitalised waterpin points

Let us look first at the red curve:

The drinking water used in the test had an initial value of 25 CFU/ml. After the water was

allowed to stand for three days at room temperature, the total microbe count was 160.

According to drinking-water regulations, this water would be only conditionally potable. The

microbe count rose in two additional days to 200 and after 30 days of storage had reached

210 CFU per ml. Thus in 30 days the total microbe count in the unrevitalised drinking water

rose by a factor of eight.

Let us look now at the blue and green curves:

Part of the water mentioned was subjected to revitalisation in a parallel revitalisation experi-

ment, in which the so-called Grander® Energy Rod was placed for about 10 seconds in a

volume of 500 ml water and then removed. (The rod, of course, had been sterilised.)

Five days after this revitalisation, the number the pin points had risen to 650. The “wild”

colonies had dropped to 10. After this maximum, both the pin points and the “wild”

colonies declined. After 20 days the number of “wild” colonies had dropped to zero;

after 30 days no pin points were detected.

Summary

This report is intended to demonstrate the significance

of Grander® Technology in the preservation of drinking

water. With the exception of Grander® Technology, I am

aware of no process that is capable of preserving drinking

water without the addition of chemicals or the implemen-

tation of physical measures. The preservation of drinking

water is probably the most important task facing us in the

new millennium.

350

20 21

Phot

os:

Dr.

Fel

sch

To answer this question, an effectiveness test was per-formed on Grander® revitalisation equipment, some of which was more than 10 years old. Pin point for-

mation was chosen as a method of establishing proof.

Up to now, there has been no method capable of mea-suring information transmission directly. Thus it has not been possible to determine the effectiveness of Grander®

revitalisation equipment using either electric or electronic instruments.

One method for determining the effectiveness of Grand-er® Technology is by monitoring pin point formation. The pin point method determines whether the water structure altered by Grander® Technology is capable of effecting “wild” colonies in such a way that their appearance changes to that of pin points. The advantages of this method are twofold: first, the revitalisation of living organisms – namely, bacte-

ria – can be measured; second, the pin point analysis can be conducted along the lines of standardised and generally recognised bacteriological methods.

The Developmental History ofGrander

® Revitalisation Equipment

The first equipment of this kind (1989-1993) consisted of bonded plastic pipes suitable for drinking water with external brass cartridges filled with Grander® Information Water.

Since 1994 Original Grander® revitalisation equipment has been manufactured in an integrated unit construction of stainless steel. There are no longer external brass cartridges but rather internal chambers filled with Original Grander® Information Water.

How Long Is Original Grander® Revitalisation Equipment Effective?

The Active Life-Span of Grander® Revitalisers

The product description of Grander® water revitalisation equipment states the following: Grand-er® revitalisation equipment operates with natural energy, without electricity,

without additives and is maintenance and service free. This raises the question of how long Grander® equipment, which has no replacement parts and is “only” filled with

Grander® Information Water, remains effective?

22 23

Description of the Effectiveness Test

All the equipment tested had been used in practical applications; that means that until its testing it was installed in drinking-water pipes. After being dismantled, the equip-ment was brought to the laboratory.

• The test began on March 22, 2000.

• The dismantled Grander® revitalisation equipment was first rinsed with scalding hot, non-revitalised water in order to cleanse it of any attached impurities and to remove any airborne microbes that might have entered the equipment pipes during dismantling or transport.

• After the equipment had been cooled, non-revitalised drinking water (from St Ulrich am Pillersee) was run through the equipment for 25 seconds at a rate of 1 l/s.

• Afterwards, the equipment that had been filled with this water was allowed to stand for 15 minutes. This method was chosen because it reflects household practice and simulates the typical practice of water usage.

Non-revitalised water from St Ulrich am Piller-

see. Sample of March 22, 2000. 3 CFU/ml.

After 48 hours of incubation, the “wild” colonies

have reached their full size. The water has not been

revitalised (no pin points).

Colony Forming Units in

Non-revitalised Water

1 ml of undiluted liquid was drawn through a membrane filter impermeable to bacteria (pore size .45 µm, with counting grid). This was then incubated on standard nutrient agar with added TTC at 22 degrees Cel-sius.

• When the 15 minutes were over, the water that remained in each revitaliser was poured into a sterilised 400-ml beaker where the volume was determined. The results were as follows:

Revitalisers made in 1989, blue plastic pipe: 223 ml

Revitalisers made in 1991, green plastic pipe: 225 ml

Revitalisers made in 1997, stainless steel: 387 ml

• These beakers were then sealed and stored in such a way that proximity revitalisation by Grander® equipment or Original Grander® drinking water was impossible.

• At the same time, in order to arrive at a base bacteriologi-cal value, the non-revitalised water from St Ulrich was sub-jected to bacteriological examination. This non-revitalised water was also poured into a 400-ml beaker and allowed to stand.

• The first bacteriological examination of the individual beak-ers was made after 24 hours and the second after 48 hours (these are depicted above).

Results of the Effective Period Tests

When the results of the pin point tests are compared, the following conclusions can be drawn:

• In this test, all three revitalisers completely revitalised the drinking water from St Ulrich am Pillersee. After an incubation period of 48 hours at 22 degrees Celsius no “wild” colonies (large mother colonies) were seen on the filter. There were only pin points.

22 23

Effectiveness Test of Three Different Grander® Models

The effectiveness of three older models of Grander®

water revitalisers was tested with regard to the formation

of pin points.

Grander® Water Revitaliser Made in 1989As the illustration shows, production

checks were made as early as 1989. The

revitalisation equipment was subjected to

a pressure of up to 12 bars and this was

noted on a sticker with the date (October

25, 1989) and a signature. This permitted

the age of the equipment to be deter-

mined exactly to the day.

After 48 hours

incubation at 22° C.

No mother colonies,

only pin point

Grander® Water Revitaliser Made in 1991The openings where the Information

Water was filled into the cartridges were

sealed, and these seals have not been

broken. The pressure-test sticker is miss-

ing from this equipment, but on the

brass cartridge itself a note has been

made with a felt-tip pen: “Installed on

April 8, 1991”.

After 48 hours:

only pin points

Grander® Water Revitaliser Made in 1997Current equipment made of stainless

steel has integrated chambers filled

with Grander® Information Water.

In flowing through the equipment,

the water to be revitalised comes into

indirect contact with the Information

Water in the chambers.

After 48 hours:

countless pin points

Final Assessment

Based on these results, the conclusion may be drawn that

Grander® revitalisation equipment keeps its powers of

revitalisation forever.

Since 1993 I have conducted about 2,000 water tests where

Grander® revitalisation equipment was installed. At

these installations, some of which were of an industrial

nature, there were demonstrable improvements in water

quality and reduction in the use of chemicals and envi-

ronmental damage. A positive pin point test always went

hand in hand with a measurable alleviation of existing

water problems.

In many of these examinations, both internal company

testing as well as external laboratories were involved.

The technical measurements confirmed both the exis-

tence of pin point formation as well as an improvement

in water quality.

• Comparing the revitalisation equipment of the first generation made in 1989 (with blue plastic pipe) with the modern integrated unit equipment of stainless steel (made in 1997) no significant difference in the number the pin points could be determined. It should be pointed out that in bacteriological tests the results can vary by plus or minus 15% to 20%.

• The blind test results: the non-revitalised drinking water from St Ulrich am Pillersee was filled into a 400ml container and after 48 hours the bacteria count determined. Result: two “wild colonies” and 13 pin points. This shows, that in a laboratory, where there is extensive work with revitalised water, it is impossible to rule out some minimal “proximity revitalisation”.

Blind test: Bacterial result of the non-revitalised water

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